AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54561


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54561.t1 MTR_4g067150 97.727 264 6 0 1 264 1 264 0.0 529
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54561.t1 AT3G59400 60.847 189 67 3 79 264 81 265 2.05e-75 230

Find 64 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGTTAAATAATTTCTTT+TGG 0.283862 4.3:-37229508 None:intergenic
GGTTATGTTGGTGGGGTTGA+TGG 0.305251 4.3:-37229183 None:intergenic
TTGTCTCTTCATCGGCTTCT+CGG 0.317241 4.3:-37229337 None:intergenic
TTGGTGGGGTTGATGGTAGT+TGG 0.324435 4.3:-37229176 None:intergenic
GTCAGAGGTAAATGACCTTC+TGG 0.364131 4.3:-37229656 None:intergenic
ATGGTGTGGATGATGTTGTT+TGG 0.368599 4.3:-37229253 None:intergenic
GACCATAGTGACAACAAGTT+TGG 0.377134 4.3:+37229476 MS.gene54561:CDS
GACGAAGTTGAAGTTTCATT+TGG 0.378588 4.3:+37229746 MS.gene54561:CDS
AGAAAATGTGACAGAGGAGT+TGG 0.387476 4.3:-37229225 None:intergenic
TTGAATTGAGTGAAGAGAAT+TGG 0.413727 4.3:-37229072 None:intergenic
TTTGGTGGCACTGAGGGAAA+TGG 0.415272 4.3:+37229764 MS.gene54561:CDS
TGGTGTGGATGATGTTGTTT+GGG 0.423170 4.3:-37229252 None:intergenic
TGGAAGAGGAAGAGTGGGAA+AGG 0.433263 4.3:-37229205 None:intergenic
AGGAGTTGGAAGAGGAAGAG+TGG 0.435674 4.3:-37229211 None:intergenic
TCAAAACAATTGACCAGCTC+TGG 0.440096 4.3:+37229450 MS.gene54561:CDS
ATCCAAACTTGTTGTCACTA+TGG 0.441920 4.3:-37229478 None:intergenic
GGTTGATGGTAGTTGGTTTG+AGG 0.455669 4.3:-37229169 None:intergenic
TCTGAGCTGCTTCGCCTGCT+AGG 0.457028 4.3:-37229376 None:intergenic
GGAGTTGGAAGAGGAAGAGT+GGG 0.459383 4.3:-37229210 None:intergenic
CTGGAGGAGTTCAAAGGAGC+TGG 0.460854 4.3:-37229291 None:intergenic
ATGAAACTTCAACTTCGTCT+TGG 0.463961 4.3:-37229742 None:intergenic
CGTCTTCTCATCGTCCTAGC+AGG 0.467340 4.3:+37229362 MS.gene54561:CDS
CACTATGGTCTCTCCAGAGC+TGG 0.482449 4.3:-37229463 None:intergenic
CTTCACCAAGTTCTTCATCA+AGG 0.483845 4.3:+37229541 MS.gene54561:CDS
GTGGGAAAGGGTTATGTTGG+TGG 0.485083 4.3:-37229192 None:intergenic
GTTTCATTTGGTGGCACTGA+GGG 0.487844 4.3:+37229758 MS.gene54561:CDS
TGTGACAGAGGAGTTGGAAG+AGG 0.488011 4.3:-37229219 None:intergenic
ACCAAGTTCTTCATCAAGGT+TGG 0.494609 4.3:+37229545 MS.gene54561:CDS
AAAAGATTCATCAAAACCAT+TGG 0.496372 4.3:+37229802 MS.gene54561:CDS
AGTTCTTCATCAAGGTTGGT+TGG 0.506590 4.3:+37229549 MS.gene54561:CDS
TCTTGGACTGCATCAAAAGC+TGG 0.506697 4.3:-37229725 None:intergenic
GGAAGAGGAAGAGTGGGAAA+GGG 0.507715 4.3:-37229204 None:intergenic
GGCGAAGCAGCTCAGAAGCG+TGG 0.511684 4.3:+37229383 MS.gene54561:CDS
CTGGTTTGAGAGGTTGATGA+TGG 0.511778 4.3:-37229272 None:intergenic
AAGACGTCTTGTCTCTTCAT+CGG 0.512265 4.3:-37229345 None:intergenic
AGATGGAACAATATAACTAT+AGG 0.512780 4.3:+37229591 MS.gene54561:CDS
TTGAAGATCTTGGTAGCCAA+TGG 0.513830 4.3:-37229818 None:intergenic
TGGGAAAGGGTTATGTTGGT+GGG 0.515839 4.3:-37229191 None:intergenic
AGAGTGGGAAAGGGTTATGT+TGG 0.523394 4.3:-37229195 None:intergenic
TTCAAAGGAGCTGGTTTGAG+AGG 0.533319 4.3:-37229282 None:intergenic
GAAGTTGAAGTTTCATTTGG+TGG 0.538306 4.3:+37229749 MS.gene54561:CDS
ACCAACCTTGATGAAGAACT+TGG 0.545583 4.3:-37229546 None:intergenic
CCAAAAGAAATTATTTAACA+AGG 0.554192 4.3:+37229508 MS.gene54561:CDS
TTGAGAGGTTGATGATGGTG+TGG 0.562770 4.3:-37229267 None:intergenic
CAGTTTCAGAAGGGTAGTAG+TGG 0.564931 4.3:-37229115 None:intergenic
GGGAAAGGGTTATGTTGGTG+GGG 0.565442 4.3:-37229190 None:intergenic
AGTGAGGATGAAAGTGTGGA+TGG 0.569347 4.3:-37229143 None:intergenic
TGGCTGAGAGATGCTGCTGG+AGG 0.581059 4.3:-37229307 None:intergenic
GAAGAAGCTTGACACTGAGA+TGG 0.583534 4.3:+37229574 MS.gene54561:CDS
GGAGATAGAAAATGTGACAG+AGG 0.591660 4.3:-37229231 None:intergenic
GATGGAGGAGCAGTTTCAGA+AGG 0.592373 4.3:-37229125 None:intergenic
GAGGGAAATGGAGCACTCAA+AGG 0.594924 4.3:+37229776 MS.gene54561:CDS
AATTGAGTGAAGAGAATTGG+TGG 0.601375 4.3:-37229069 None:intergenic
GAGGATGAAAGTGTGGATGG+AGG 0.603905 4.3:-37229140 None:intergenic
CCTCTGAGTGCATTTGTCAG+AGG 0.605940 4.3:-37229671 None:intergenic
ATGCTGCTGGAGGAGTTCAA+AGG 0.611446 4.3:-37229297 None:intergenic
GGACTGCATCAAAAGCTGGA+TGG 0.636421 4.3:-37229721 None:intergenic
GAAAAGTGAGGATGAAAGTG+TGG 0.636789 4.3:-37229147 None:intergenic
AGCTCCCATATAAACTCTGT+AGG 0.639673 4.3:-37229620 None:intergenic
CTCAATGATGACACTCCAGA+AGG 0.645146 4.3:+37229641 MS.gene54561:CDS
ATGGAGGAGCAGTTTCAGAA+GGG 0.648881 4.3:-37229124 None:intergenic
AGTTGGTTTGAGGAAAAGTG+AGG 0.684896 4.3:-37229159 None:intergenic
AGTTTCATTTGGTGGCACTG+AGG 0.701247 4.3:+37229757 MS.gene54561:CDS
CCTCTGACAAATGCACTCAG+AGG 0.721539 4.3:+37229671 MS.gene54561:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCAAAAGAAATTATTTAACA+AGG + chr4.3:37229508-37229527 MS.gene54561:CDS 20.0%
!!! CCTTGTTAAATAATTTCTTT+TGG - chr4.3:37229511-37229530 None:intergenic 20.0%
! AAAAGATTCATCAAAACCAT+TGG + chr4.3:37229802-37229821 MS.gene54561:CDS 25.0%
! AGATGGAACAATATAACTAT+AGG + chr4.3:37229591-37229610 MS.gene54561:CDS 25.0%
!! TTTTCCTACAGAGTTTATAT+GGG + chr4.3:37229616-37229635 MS.gene54561:CDS 25.0%
TTGAATTGAGTGAAGAGAAT+TGG - chr4.3:37229075-37229094 None:intergenic 30.0%
! CTTTTCCTACAGAGTTTATA+TGG + chr4.3:37229615-37229634 MS.gene54561:CDS 30.0%
!!! ATAGTCTGTTTTGAAGATCT+TGG - chr4.3:37229831-37229850 None:intergenic 30.0%
AATTGAGTGAAGAGAATTGG+TGG - chr4.3:37229072-37229091 None:intergenic 35.0%
ATGAAACTTCAACTTCGTCT+TGG - chr4.3:37229745-37229764 None:intergenic 35.0%
GAAGTTGAAGTTTCATTTGG+TGG + chr4.3:37229749-37229768 MS.gene54561:CDS 35.0%
GACGAAGTTGAAGTTTCATT+TGG + chr4.3:37229746-37229765 MS.gene54561:CDS 35.0%
!! ATCCAAACTTGTTGTCACTA+TGG - chr4.3:37229481-37229500 None:intergenic 35.0%
AAGACGTCTTGTCTCTTCAT+CGG - chr4.3:37229348-37229367 None:intergenic 40.0%
ACCAACCTTGATGAAGAACT+TGG - chr4.3:37229549-37229568 None:intergenic 40.0%
ACCAAGTTCTTCATCAAGGT+TGG + chr4.3:37229545-37229564 MS.gene54561:CDS 40.0%
AGAAAATGTGACAGAGGAGT+TGG - chr4.3:37229228-37229247 None:intergenic 40.0%
AGCTCCCATATAAACTCTGT+AGG - chr4.3:37229623-37229642 None:intergenic 40.0%
ATGGTGTGGATGATGTTGTT+TGG - chr4.3:37229256-37229275 None:intergenic 40.0%
CTTCACCAAGTTCTTCATCA+AGG + chr4.3:37229541-37229560 MS.gene54561:CDS 40.0%
GAAAAGTGAGGATGAAAGTG+TGG - chr4.3:37229150-37229169 None:intergenic 40.0%
GACCATAGTGACAACAAGTT+TGG + chr4.3:37229476-37229495 MS.gene54561:CDS 40.0%
GGAGATAGAAAATGTGACAG+AGG - chr4.3:37229234-37229253 None:intergenic 40.0%
TCAAAACAATTGACCAGCTC+TGG + chr4.3:37229450-37229469 MS.gene54561:CDS 40.0%
! AGTTCTTCATCAAGGTTGGT+TGG + chr4.3:37229549-37229568 MS.gene54561:CDS 40.0%
! TGGCTATGTTTTCTTCTCAG+AGG + chr4.3:37229403-37229422 MS.gene54561:CDS 40.0%
! TGGTGTGGATGATGTTGTTT+GGG - chr4.3:37229255-37229274 None:intergenic 40.0%
! TTGAAGATCTTGGTAGCCAA+TGG - chr4.3:37229821-37229840 None:intergenic 40.0%
!! AGAGCTGGTCAATTGTTTTG+AGG - chr4.3:37229451-37229470 None:intergenic 40.0%
!! AGTTGGTTTGAGGAAAAGTG+AGG - chr4.3:37229162-37229181 None:intergenic 40.0%
AGAGTGGGAAAGGGTTATGT+TGG - chr4.3:37229198-37229217 None:intergenic 45.0%
ATGGAGGAGCAGTTTCAGAA+GGG - chr4.3:37229127-37229146 None:intergenic 45.0%
CAGTTTCAGAAGGGTAGTAG+TGG - chr4.3:37229118-37229137 None:intergenic 45.0%
CTCAATGATGACACTCCAGA+AGG + chr4.3:37229641-37229660 MS.gene54561:CDS 45.0%
GAAGAAGCTTGACACTGAGA+TGG + chr4.3:37229574-37229593 MS.gene54561:CDS 45.0%
GTCAGAGGTAAATGACCTTC+TGG - chr4.3:37229659-37229678 None:intergenic 45.0%
TCTTGGACTGCATCAAAAGC+TGG - chr4.3:37229728-37229747 None:intergenic 45.0%
TGGGAAAGGGTTATGTTGGT+GGG - chr4.3:37229194-37229213 None:intergenic 45.0%
! AGTGAGGATGAAAGTGTGGA+TGG - chr4.3:37229146-37229165 None:intergenic 45.0%
! AGTTTCATTTGGTGGCACTG+AGG + chr4.3:37229757-37229776 MS.gene54561:CDS 45.0%
! GTTTCATTTGGTGGCACTGA+GGG + chr4.3:37229758-37229777 MS.gene54561:CDS 45.0%
!! ATCGGCTTCTCGGAAGTTTT+TGG - chr4.3:37229330-37229349 None:intergenic 45.0%
!! CTGGTTTGAGAGGTTGATGA+TGG - chr4.3:37229275-37229294 None:intergenic 45.0%
!! GGTTGATGGTAGTTGGTTTG+AGG - chr4.3:37229172-37229191 None:intergenic 45.0%
!! TTCAAAGGAGCTGGTTTGAG+AGG - chr4.3:37229285-37229304 None:intergenic 45.0%
!! TTGAGAGGTTGATGATGGTG+TGG - chr4.3:37229270-37229289 None:intergenic 45.0%
!! TTGTCTCTTCATCGGCTTCT+CGG - chr4.3:37229340-37229359 None:intergenic 45.0%
AGGAGTTGGAAGAGGAAGAG+TGG - chr4.3:37229214-37229233 None:intergenic 50.0%
ATGCTGCTGGAGGAGTTCAA+AGG - chr4.3:37229300-37229319 None:intergenic 50.0%
CCTCTGACAAATGCACTCAG+AGG + chr4.3:37229671-37229690 MS.gene54561:CDS 50.0%
CCTCTGAGTGCATTTGTCAG+AGG - chr4.3:37229674-37229693 None:intergenic 50.0%
GATGGAGGAGCAGTTTCAGA+AGG - chr4.3:37229128-37229147 None:intergenic 50.0%
GGAAGAGGAAGAGTGGGAAA+GGG - chr4.3:37229207-37229226 None:intergenic 50.0%
GGACTGCATCAAAAGCTGGA+TGG - chr4.3:37229724-37229743 None:intergenic 50.0%
GGAGTTGGAAGAGGAAGAGT+GGG - chr4.3:37229213-37229232 None:intergenic 50.0%
GGGAAAGGGTTATGTTGGTG+GGG - chr4.3:37229193-37229212 None:intergenic 50.0%
GTGGGAAAGGGTTATGTTGG+TGG - chr4.3:37229195-37229214 None:intergenic 50.0%
TGGAAGAGGAAGAGTGGGAA+AGG - chr4.3:37229208-37229227 None:intergenic 50.0%
TGTGACAGAGGAGTTGGAAG+AGG - chr4.3:37229222-37229241 None:intergenic 50.0%
! GAGGATGAAAGTGTGGATGG+AGG - chr4.3:37229143-37229162 None:intergenic 50.0%
! GAGGGAAATGGAGCACTCAA+AGG + chr4.3:37229776-37229795 MS.gene54561:CDS 50.0%
! GGTTATGTTGGTGGGGTTGA+TGG - chr4.3:37229186-37229205 None:intergenic 50.0%
! TTTGGTGGCACTGAGGGAAA+TGG + chr4.3:37229764-37229783 MS.gene54561:CDS 50.0%
!! TTGGTGGGGTTGATGGTAGT+TGG - chr4.3:37229179-37229198 None:intergenic 50.0%
!! TTTTGGCTGAGAGATGCTGC+TGG - chr4.3:37229313-37229332 None:intergenic 50.0%
CACTATGGTCTCTCCAGAGC+TGG - chr4.3:37229466-37229485 None:intergenic 55.0%
CGTCTTCTCATCGTCCTAGC+AGG + chr4.3:37229362-37229381 MS.gene54561:CDS 55.0%
CTGGAGGAGTTCAAAGGAGC+TGG - chr4.3:37229294-37229313 None:intergenic 55.0%
TCTGAGCTGCTTCGCCTGCT+AGG - chr4.3:37229379-37229398 None:intergenic 60.0%
TGGCTGAGAGATGCTGCTGG+AGG - chr4.3:37229310-37229329 None:intergenic 60.0%
GGCGAAGCAGCTCAGAAGCG+TGG + chr4.3:37229383-37229402 MS.gene54561:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 37229065 37229859 37229065 ID=MS.gene54561
chr4.3 mRNA 37229065 37229859 37229065 ID=MS.gene54561.t1;Parent=MS.gene54561
chr4.3 exon 37229065 37229859 37229065 ID=MS.gene54561.t1.exon1;Parent=MS.gene54561.t1
chr4.3 CDS 37229065 37229859 37229065 ID=cds.MS.gene54561.t1;Parent=MS.gene54561.t1
Gene Sequence

>MS.gene54561

ATGGCCACCAATTCTCTTCACTCAATTCAACATCATTCTCTCAGCAAACGCCACTACTACCCTTCTGAAACTGCTCCTCCATCCACACTTTCATCCTCACTTTTCCTCAAACCAACTACCATCAACCCCACCAACATAACCCTTTCCCACTCTTCCTCTTCCAACTCCTCTGTCACATTTTCTATCTCCCAAACAACATCATCCACACCATCATCAACCTCTCAAACCAGCTCCTTTGAACTCCTCCAGCAGCATCTCTCAGCCAAAAACTTCCGAGAAGCCGATGAAGAGACAAGACGTCTTCTCATCGTCCTAGCAGGCGAAGCAGCTCAGAAGCGTGGCTATGTTTTCTTCTCAGAGGTTCAGTTCATCTCAGAAGCAGACCTCAAAACAATTGACCAGCTCTGGAGAGACCATAGTGACAACAAGTTTGGATACAGTGTCCAAAAGAAATTATTTAACAAGGCTAAAAAAGACTTCACCAAGTTCTTCATCAAGGTTGGTTGGATGAAGAAGCTTGACACTGAGATGGAACAATATAACTATAGGTCTTTTCCTACAGAGTTTATATGGGAGCTCAATGATGACACTCCAGAAGGTCATTTACCTCTGACAAATGCACTCAGAGGAACACAGTTGCTAAGTTATATTTTTAACCATCCAGCTTTTGATGCAGTCCAAGACGAAGTTGAAGTTTCATTTGGTGGCACTGAGGGAAATGGAGCACTCAAAGGATTAAAAGATTCATCAAAACCATTGGCTACCAAGATCTTCAAAACAGACTATAGTTTCTGA

Protein sequence

>MS.gene54561.t1

MATNSLHSIQHHSLSKRHYYPSETAPPSTLSSSLFLKPTTINPTNITLSHSSSSNSSVTFSISQTTSSTPSSTSQTSSFELLQQHLSAKNFREADEETRRLLIVLAGEAAQKRGYVFFSEVQFISEADLKTIDQLWRDHSDNKFGYSVQKKLFNKAKKDFTKFFIKVGWMKKLDTEMEQYNYRSFPTEFIWELNDDTPEGHLPLTNALRGTQLLSYIFNHPAFDAVQDEVEVSFGGTEGNGALKGLKDSSKPLATKIFKTDYSF