AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0240600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0240600 MtrunA17_Chr2g0292021 83.607 122 20 0 1 122 1 122 2.14e-63 201
Msa0240600 MtrunA17_Chr2g0292011 82.787 122 21 0 1 122 1 122 6.98e-63 199
Msa0240600 MtrunA17_Chr7g0237461 81.967 122 22 0 1 122 1 122 2.37e-62 198
Msa0240600 MtrunA17_Chr8g0347561 66.393 122 41 0 1 122 22 143 1.30e-50 167
Msa0240600 MtrunA17_Chr7g0262201 55.462 119 53 0 4 122 1 119 1.41e-42 145
Msa0240600 MtrunA17_Chr7g0262211 54.098 122 56 0 1 122 24 145 1.44e-42 146
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0240600 AT2G20340.2 47.541 122 64 0 1 122 9 130 3.45e-39 136
Msa0240600 AT2G20340.1 47.541 122 64 0 1 122 9 130 7.20e-39 136
Msa0240600 AT4G28680.6 50.000 126 59 1 1 122 48 173 2.58e-37 130
Msa0240600 AT4G28680.7 50.000 126 59 1 1 122 48 173 4.52e-37 130
Msa0240600 AT4G28680.4 50.000 126 59 1 1 122 48 173 4.74e-37 132
Msa0240600 AT4G28680.3 50.000 126 59 1 1 122 57 182 5.39e-37 132
Msa0240600 AT4G28680.2 50.000 126 59 1 1 122 57 182 5.84e-37 132
Msa0240600 AT4G28680.1 50.000 126 59 1 1 122 57 182 6.56e-37 131
Msa0240600 AT4G28680.5 50.000 126 59 1 1 122 48 173 9.30e-37 131

Find 20 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGCACAGCAGGGTTCTT+AGG 0.266911 2_2:-15951766 Msa0240600:CDS
GGAAAATAAGCAAAGTAATT+AGG 0.271671 2_2:+15951796 None:intergenic
CAAGGATGGTTTCAATATAT+AGG 0.339574 2_2:+15951866 None:intergenic
CCTGCTGTGCTACCACTTGA+TGG 0.350739 2_2:+15951775 None:intergenic
TTGAATTCTGTAGTATCTAG+TGG 0.355450 2_2:+15952022 None:intergenic
TGAATGTTGTTGGATTTAAC+TGG 0.365518 2_2:-15951723 Msa0240600:CDS
TGGTTTCAATATATAGGTTC+AGG 0.384217 2_2:+15951872 None:intergenic
CCATCAAGTGGTAGCACAGC+AGG 0.386882 2_2:-15951775 Msa0240600:CDS
AGCACTGGATTGAATGTTGT+TGG 0.421179 2_2:-15951733 Msa0240600:CDS
CTTTGCCAATGTATTATACC+TGG 0.424206 2_2:+15951820 None:intergenic
TTAGGAGAAATGTTAAGCAC+TGG 0.472185 2_2:-15951748 Msa0240600:CDS
AGTTTGGTTCTACTTGACTT+AGG 0.478980 2_2:+15951933 None:intergenic
TATATAGGTTCAGGATTTGA+AGG 0.481064 2_2:+15951881 None:intergenic
TGAATTCTGTAGTATCTAGT+GGG 0.505218 2_2:+15952023 None:intergenic
GGATTTGAAGGTGCAAAAGA+AGG 0.506622 2_2:+15951893 None:intergenic
GGTTCTACTTGACTTAGGAC+AGG 0.523508 2_2:+15951938 None:intergenic
TTTGCCAATGTATTATACCT+GGG 0.574268 2_2:+15951821 None:intergenic
TAATCCCAGGTATAATACAT+TGG 0.593258 2_2:-15951825 Msa0240600:CDS
CATCAAGTGGTAGCACAGCA+GGG 0.607312 2_2:-15951774 Msa0240600:CDS
ACTACAGAATTCAAGAAACA+AGG 0.665050 2_2:-15952013 Msa0240600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTTCATAATAATCAGCT+AGG + chr2_2:15951731-15951750 None:intergenic 25.0%
!!! TTTTTGATAATCTTCAAGGA+TGG + chr2_2:15951851-15951870 None:intergenic 25.0%
!!! TGTGTTTTTGATAATCTTCA+AGG + chr2_2:15951855-15951874 None:intergenic 25.0%
! GGAAAATAAGCAAAGTAATT+AGG + chr2_2:15951907-15951926 None:intergenic 25.0%
TGAATTCTGTAGTATCTAGT+GGG + chr2_2:15951680-15951699 None:intergenic 30.0%
TTGAATTCTGTAGTATCTAG+TGG + chr2_2:15951681-15951700 None:intergenic 30.0%
ACTACAGAATTCAAGAAACA+AGG - chr2_2:15951687-15951706 Msa0240600:CDS 30.0%
!!! AACAGTTTTTTGAGGTAGTT+TGG + chr2_2:15951786-15951805 None:intergenic 30.0%
!!! AAGAAGGTAACAGTTTTTTG+AGG + chr2_2:15951794-15951813 None:intergenic 30.0%
TATATAGGTTCAGGATTTGA+AGG + chr2_2:15951822-15951841 None:intergenic 30.0%
TGGTTTCAATATATAGGTTC+AGG + chr2_2:15951831-15951850 None:intergenic 30.0%
CAAGGATGGTTTCAATATAT+AGG + chr2_2:15951837-15951856 None:intergenic 30.0%
TATCAAAAACACATAATCCC+AGG - chr2_2:15951862-15951881 Msa0240600:CDS 30.0%
TAATCCCAGGTATAATACAT+TGG - chr2_2:15951875-15951894 Msa0240600:CDS 30.0%
TTTGCCAATGTATTATACCT+GGG + chr2_2:15951882-15951901 None:intergenic 30.0%
!! TTTGCTTATTTTCCATCAAG+TGG - chr2_2:15951913-15951932 Msa0240600:CDS 30.0%
TGAATGTTGTTGGATTTAAC+TGG - chr2_2:15951977-15951996 Msa0240600:CDS 30.0%
!! AGTTTGGTTCTACTTGACTT+AGG + chr2_2:15951770-15951789 None:intergenic 35.0%
CTTTGCCAATGTATTATACC+TGG + chr2_2:15951883-15951902 None:intergenic 35.0%
TTAGGAGAAATGTTAAGCAC+TGG - chr2_2:15951952-15951971 Msa0240600:CDS 35.0%
! GGATTTGAAGGTGCAAAAGA+AGG + chr2_2:15951810-15951829 None:intergenic 40.0%
! AGCACTGGATTGAATGTTGT+TGG - chr2_2:15951967-15951986 Msa0240600:CDS 40.0%
!!! TTTTCTAGTTCTGTTGCAGC+TGG + chr2_2:15952012-15952031 None:intergenic 40.0%
GGTTCTACTTGACTTAGGAC+AGG + chr2_2:15951765-15951784 None:intergenic 45.0%
CATCAAGTGGTAGCACAGCA+GGG - chr2_2:15951926-15951945 Msa0240600:intron 50.0%
CCTGCTGTGCTACCACTTGA+TGG + chr2_2:15951928-15951947 None:intergenic 55.0%
CCATCAAGTGGTAGCACAGC+AGG - chr2_2:15951925-15951944 Msa0240600:intron 55.0%
GGTAGCACAGCAGGGTTCTT+AGG - chr2_2:15951934-15951953 Msa0240600:intron 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 15951672 15952050 15951672 ID=Msa0240600;Name=Msa0240600
chr2_2 mRNA 15951672 15952050 15951672 ID=Msa0240600-mRNA-1;Parent=Msa0240600;Name=Msa0240600-mRNA-1;_AED=0.45;_eAED=0.54;_QI=0|0|0|1|0|0|2|0|124
chr2_2 exon 15951946 15952050 15951946 ID=Msa0240600-mRNA-1:exon:6518;Parent=Msa0240600-mRNA-1
chr2_2 exon 15951672 15951941 15951672 ID=Msa0240600-mRNA-1:exon:6517;Parent=Msa0240600-mRNA-1
chr2_2 CDS 15951946 15952050 15951946 ID=Msa0240600-mRNA-1:cds;Parent=Msa0240600-mRNA-1
chr2_2 CDS 15951672 15951941 15951672 ID=Msa0240600-mRNA-1:cds;Parent=Msa0240600-mRNA-1
Gene Sequence

>Msa0240600

ATGAACCCACTAGATACTACAGAATTCAAGAAACAAGGTCACATGATCATTGATTTCCTAGCTGATTATTATGAAAACATTTCAAACTATCCTGTCCTAAGTCAAAACCAAACTACCTCAAAAAACTGTTACCTTCTTTTGCACCTTCAAATCCTGAACCTATATATTGAAACCATCCTTGAAGATTATCAAAAACACATAATCCCAGGTATAATACATTGGCAAAGTCCTAATTACTTTGCTTATTTTCCATCAAGTGGTAGCACAGCAGGGTTCTTAGGAGAAATGTTAAGCACTGGATTGAATGTTGTTGGATTTAACTGGATTTCATCACCAGCTGCAACAGAACTAGAAAATGTTGTTATAATAAACTAA

Protein sequence

>Msa0240600

MNPLDTTEFKKQGHMIIDFLADYYENISNYPVLSQNQTTSKNCYLLLHLQILNLYIETILEDYQKHIIPGIIHWQSPNYFAYFPSSGSTAGFLGEMLSTGLNVVGFNWISSPAATELENVVIIN*