AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0195150


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195150 MtrunA17_Chr2g0292021 99.265 136 1 0 1 136 1 136 1.45e-95 285
Msa0195150 MtrunA17_Chr2g0292011 98.529 136 2 0 1 136 1 136 5.68e-95 283
Msa0195150 MtrunA17_Chr7g0237461 97.059 136 4 0 1 136 1 136 5.84e-94 280
Msa0195150 MtrunA17_Chr8g0347561 75.735 136 33 0 1 136 22 157 3.13e-73 227
Msa0195150 MtrunA17_Chr7g0262211 62.044 137 52 0 1 137 24 160 2.98e-61 196
Msa0195150 MtrunA17_Chr7g0262201 63.433 134 49 0 4 137 1 134 4.71e-61 194
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195150 AT2G20340.2 56.204 137 60 0 1 137 9 145 2.35e-59 190
Msa0195150 AT2G20340.1 56.204 137 60 0 1 137 9 145 5.89e-59 189
Msa0195150 AT4G28680.6 57.447 141 56 1 1 137 48 188 5.49e-57 182
Msa0195150 AT4G28680.7 57.447 141 56 1 1 137 48 188 1.07e-56 182
Msa0195150 AT4G28680.4 57.447 141 56 1 1 137 48 188 4.40e-56 183
Msa0195150 AT4G28680.1 57.447 141 56 1 1 137 57 197 5.82e-56 183
Msa0195150 AT4G28680.2 57.447 141 56 1 1 137 57 197 6.15e-56 183
Msa0195150 AT4G28680.3 57.447 141 56 1 1 137 57 197 6.28e-56 183
Msa0195150 AT4G28680.5 57.447 141 56 1 1 137 48 188 1.10e-55 182

Find 23 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGCACAGCAGGGTTCTT+AGG 0.266911 2_1:-11893078 Msa0195150:CDS
GGAAAATAAGCAAAGTAATT+AGG 0.271671 2_1:+11893108 None:intergenic
TGAACGTTGTTGGATTTAAC+TGG 0.304439 2_1:-11893035 Msa0195150:CDS
TAGAAAATGTTGTTATAGAT+TGG 0.319360 2_1:-11892987 Msa0195150:CDS
CCTGCTGTGCTACCACTTGA+TGG 0.350739 2_1:+11893087 None:intergenic
TTGAATTCTGTAGTATCTAG+TGG 0.355450 2_1:+11893330 None:intergenic
CCATCAAGTGGTAGCACAGC+AGG 0.386882 2_1:-11893087 Msa0195150:CDS
AGGATGGTTTCAATAGGTTC+AGG 0.403995 2_1:+11893180 None:intergenic
AGCACTGGATTGAACGTTGT+TGG 0.408006 2_1:-11893045 Msa0195150:CDS
TCTTCAAGGATGGTTTCAAT+AGG 0.409255 2_1:+11893174 None:intergenic
CTTTGCCAATGTGTTATACC+TGG 0.443144 2_1:+11893132 None:intergenic
TTAGGAGAAATGTTAAGCAC+TGG 0.472185 2_1:-11893060 Msa0195150:CDS
AGTTTGGTTCTACTTGACTT+AGG 0.478980 2_1:+11893241 None:intergenic
TGAATTCTGTAGTATCTAGT+GGG 0.505218 2_1:+11893331 None:intergenic
GGATTTGAAGGTGCAAAAGA+AGG 0.506622 2_1:+11893201 None:intergenic
GGTTCTACTTGACTTAGGAC+AGG 0.513633 2_1:+11893246 None:intergenic
TCAATAGGTTCAGGATTTGA+AGG 0.516728 2_1:+11893189 None:intergenic
AATGTTGTTATAGATTGGCT+TGG 0.556855 2_1:-11892982 Msa0195150:CDS
CTACTTGACTTAGGACAGGA+TGG 0.557815 2_1:+11893250 None:intergenic
TTTGCCAATGTGTTATACCT+GGG 0.567911 2_1:+11893133 None:intergenic
CATCAAGTGGTAGCACAGCA+GGG 0.607312 2_1:-11893086 Msa0195150:CDS
TAATCCCAGGTATAACACAT+TGG 0.657713 2_1:-11893137 Msa0195150:CDS
ACTACAGAATTCAAGAAACA+AGG 0.665050 2_1:-11893321 Msa0195150:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGAAATGTTTTCATAATAAT+CGG + chr2_1:11893009-11893028 None:intergenic 15.0%
!! TAGAAAATGTTGTTATAGAT+TGG - chr2_1:11893294-11893313 Msa0195150:CDS 20.0%
!!! TTTTTGATAATCTTCAAGGA+TGG + chr2_1:11893120-11893139 None:intergenic 25.0%
!!! TGTGTTTTTGATAATCTTCA+AGG + chr2_1:11893124-11893143 None:intergenic 25.0%
! GGAAAATAAGCAAAGTAATT+AGG + chr2_1:11893176-11893195 None:intergenic 25.0%
TGAATTCTGTAGTATCTAGT+GGG + chr2_1:11892953-11892972 None:intergenic 30.0%
TTGAATTCTGTAGTATCTAG+TGG + chr2_1:11892954-11892973 None:intergenic 30.0%
ACTACAGAATTCAAGAAACA+AGG - chr2_1:11892960-11892979 Msa0195150:CDS 30.0%
! TGTTTTCATAATAATCGGCT+AGG + chr2_1:11893004-11893023 None:intergenic 30.0%
!!! AACAGTTTTTTGAGGTAGTT+TGG + chr2_1:11893059-11893078 None:intergenic 30.0%
!!! AAGAAGGTAACAGTTTTTTG+AGG + chr2_1:11893067-11893086 None:intergenic 30.0%
TATCAAAAACACATAATCCC+AGG - chr2_1:11893131-11893150 Msa0195150:CDS 30.0%
!! TTTGCTTATTTTCCATCAAG+TGG - chr2_1:11893182-11893201 Msa0195150:CDS 30.0%
! AATGTTGTTATAGATTGGCT+TGG - chr2_1:11893299-11893318 Msa0195150:CDS 30.0%
!! AGTTTGGTTCTACTTGACTT+AGG + chr2_1:11893043-11893062 None:intergenic 35.0%
TCAATAGGTTCAGGATTTGA+AGG + chr2_1:11893095-11893114 None:intergenic 35.0%
TCTTCAAGGATGGTTTCAAT+AGG + chr2_1:11893110-11893129 None:intergenic 35.0%
TAATCCCAGGTATAACACAT+TGG - chr2_1:11893144-11893163 Msa0195150:CDS 35.0%
TTTGCCAATGTGTTATACCT+GGG + chr2_1:11893151-11893170 None:intergenic 35.0%
TTAGGAGAAATGTTAAGCAC+TGG - chr2_1:11893221-11893240 Msa0195150:CDS 35.0%
TGAACGTTGTTGGATTTAAC+TGG - chr2_1:11893246-11893265 Msa0195150:CDS 35.0%
! GGATTTGAAGGTGCAAAAGA+AGG + chr2_1:11893083-11893102 None:intergenic 40.0%
AGGATGGTTTCAATAGGTTC+AGG + chr2_1:11893104-11893123 None:intergenic 40.0%
CTTTGCCAATGTGTTATACC+TGG + chr2_1:11893152-11893171 None:intergenic 40.0%
!!! TTTTCTAGTTCTGTAGCAGC+TGG + chr2_1:11893281-11893300 None:intergenic 40.0%
CTACTTGACTTAGGACAGGA+TGG + chr2_1:11893034-11893053 None:intergenic 45.0%
GGTTCTACTTGACTTAGGAC+AGG + chr2_1:11893038-11893057 None:intergenic 45.0%
! AGCACTGGATTGAACGTTGT+TGG - chr2_1:11893236-11893255 Msa0195150:CDS 45.0%
CATCAAGTGGTAGCACAGCA+GGG - chr2_1:11893195-11893214 Msa0195150:CDS 50.0%
CCTGCTGTGCTACCACTTGA+TGG + chr2_1:11893197-11893216 None:intergenic 55.0%
CCATCAAGTGGTAGCACAGC+AGG - chr2_1:11893194-11893213 Msa0195150:CDS 55.0%
GGTAGCACAGCAGGGTTCTT+AGG - chr2_1:11893203-11893222 Msa0195150:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 11892945 11893358 11892945 ID=Msa0195150;Name=Msa0195150
chr2_1 mRNA 11892945 11893358 11892945 ID=Msa0195150-mRNA-1;Parent=Msa0195150;Name=Msa0195150-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|137
chr2_1 exon 11892945 11893358 11892945 ID=Msa0195150-mRNA-1:exon:5226;Parent=Msa0195150-mRNA-1
chr2_1 CDS 11892945 11893358 11892945 ID=Msa0195150-mRNA-1:cds;Parent=Msa0195150-mRNA-1
Gene Sequence

>Msa0195150

ATGAACCCACTAGATACTACAGAATTCAAGAAACAAGGTCACATGATCATTGATTTCCTAGCCGATTATTATGAAAACATTTCAAACCATCCTGTCCTAAGTCAAGTAGAACCAAACTACCTCAAAAAACTGTTACCTTCTTTTGCACCTTCAAATCCTGAACCTATTGAAACCATCCTTGAAGATTATCAAAAACACATAATCCCAGGTATAACACATTGGCAAAGTCCTAATTACTTTGCTTATTTTCCATCAAGTGGTAGCACAGCAGGGTTCTTAGGAGAAATGTTAAGCACTGGATTGAACGTTGTTGGATTTAACTGGATTTCATCACCAGCTGCTACAGAACTAGAAAATGTTGTTATAGATTGGCTTGGTGAAATTTTGAATCTTCCTAAGAGTTTTCTTTCGTAA

Protein sequence

>Msa0195150

MNPLDTTEFKKQGHMIIDFLADYYENISNHPVLSQVEPNYLKKLLPSFAPSNPEPIETILEDYQKHIIPGITHWQSPNYFAYFPSSGSTAGFLGEMLSTGLNVVGFNWISSPAATELENVVIDWLGEILNLPKSFLS*