AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92305


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92305.t1 MTR_5g006130 97.143 210 5 1 1 210 1 209 1.21e-149 414
MS.gene92305.t1 MTR_1g104630 67.281 217 40 3 1 210 1 193 7.26e-95 275
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92305.t1 AT1G03130 73.684 209 49 3 3 210 1 204 2.45e-105 302
MS.gene92305.t1 AT4G02770 72.770 213 48 4 3 210 1 208 7.42e-102 293

Find 60 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATTGAAGTTAAGTTTAC+TGG 0.207430 5.1:+1045714 MS.gene92305:CDS
AAGGAGTTGGTCAGAATTTC+AGG 0.217942 5.1:+1045670 MS.gene92305:CDS
AAAGAACAGTGTTTGGCTTT+AGG 0.250321 5.1:+1045525 MS.gene92305:CDS
GATGGTTGTTTCCATGGTTT+AGG 0.251347 5.1:-1045174 None:intergenic
CCAGTAAACTTAACTTCAAT+TGG 0.254455 5.1:-1045714 None:intergenic
TGGCTTTAGGAAATAGATTA+AGG 0.256198 5.1:+1045538 MS.gene92305:CDS
AACACACCTTCACCAATCTT+TGG 0.314938 5.1:+1045354 MS.gene92305:CDS
GTTAGGACCTTCTCTCATTA+TGG 0.318870 5.1:-1045484 None:intergenic
CGACCAGGGTTAACCTTCTC+AGG 0.322720 5.1:-1045645 None:intergenic
AAATATTGAACCTCACCATT+AGG 0.330180 5.1:-1045600 None:intergenic
ATGGTTGTTTCCATGGTTTA+GGG 0.335226 5.1:-1045173 None:intergenic
GGTCAGAATTTCAGGTCTAT+TGG 0.346075 5.1:+1045678 MS.gene92305:CDS
CTTGCTAACTTAAGAAGGTT+AGG 0.358826 5.1:-1045501 None:intergenic
TGTTCTTGTTGTGAACTTGA+TGG 0.369555 5.1:-1045223 None:intergenic
AAGATTGGTGAAGGTGTGTT+TGG 0.388416 5.1:-1045351 None:intergenic
AATATTGAACCTCACCATTA+GGG 0.389009 5.1:-1045599 None:intergenic
AGCAAGGAAAGAACAGTGTT+TGG 0.399243 5.1:+1045518 MS.gene92305:CDS
GAGATGAAGGATAAAGTTGA+TGG 0.402382 5.1:-1045192 None:intergenic
CTTTCCTTGCTAACTTAAGA+AGG 0.421239 5.1:-1045506 None:intergenic
GGTGTGTTTGGGTCTAGTTC+AGG 0.421350 5.1:-1045339 None:intergenic
CAGTAAACTTAACTTCAATT+GGG 0.432988 5.1:-1045713 None:intergenic
AAAGTTGATGGTTGTTTCCA+TGG 0.433796 5.1:-1045180 None:intergenic
GTTCTTGTTGTGAACTTGAT+GGG 0.443234 5.1:-1045222 None:intergenic
ATCTCAAAGATTTGTTCCTT+AGG 0.450732 5.1:-1045444 None:intergenic
GATGGGTTTGAGAGAGATGA+AGG 0.471436 5.1:-1045205 None:intergenic
TCAGGGTACACACCATCCTT+AGG 0.473133 5.1:-1045627 None:intergenic
CAAATCTTTGAGATGCCAAC+TGG 0.474824 5.1:+1045453 MS.gene92305:CDS
GTGAAACCAACTGGTGCAGC+AGG 0.478227 5.1:-1045312 None:intergenic
AGATTGGTGAAGGTGTGTTT+GGG 0.480790 5.1:-1045350 None:intergenic
GTCAACCCTTACACATCAAA+AGG 0.481319 5.1:-1045744 None:intergenic
CCAATCTTTGGAGGAAGCAC+TGG 0.483310 5.1:+1045366 MS.gene92305:CDS
CACTGGTGGTTTGTTACGTA+AGG 0.494140 5.1:+1045383 MS.gene92305:CDS
CCAGTGCTTCCTCCAAAGAT+TGG 0.498438 5.1:-1045366 None:intergenic
TCAGGTGGAGTGAAACCAAC+TGG 0.512231 5.1:-1045321 None:intergenic
TGACCAACTCCTTCACGACC+AGG 0.519461 5.1:-1045660 None:intergenic
GGTTCAATATTTGCATCCTA+AGG 0.527977 5.1:+1045611 MS.gene92305:CDS
CTAACCTTCTTAAGTTAGCA+AGG 0.531725 5.1:+1045502 MS.gene92305:CDS
GAGAGGTGGAGTGAAGAGAG+AGG 0.532656 5.1:-1045145 None:intergenic
ACACCTTCACCAATCTTTGG+AGG 0.538603 5.1:+1045357 MS.gene92305:CDS
GAAGCTCCTGCTGCACCAGT+TGG 0.541120 5.1:+1045306 MS.gene92305:CDS
ATCTTTGGAGGAAGCACTGG+TGG 0.547257 5.1:+1045369 MS.gene92305:CDS
GTGTTTGGGTCTAGTTCAGG+TGG 0.547713 5.1:-1045336 None:intergenic
CAATATTTGCATCCTAAGGA+TGG 0.551649 5.1:+1045615 MS.gene92305:CDS
GGTTTAGGGCTTGAGAGAGG+TGG 0.569137 5.1:-1045159 None:intergenic
AAGGTTAACCCTGGTCGTGA+AGG 0.571068 5.1:+1045651 MS.gene92305:CDS
CATGGTTTAGGGCTTGAGAG+AGG 0.581991 5.1:-1045162 None:intergenic
CTCTCTCAAGCCCTAAACCA+TGG 0.587901 5.1:+1045163 MS.gene92305:CDS
GATCACATGGGAGTCACCTA+AGG 0.589857 5.1:+1045428 MS.gene92305:CDS
GGATGGTGTGTACCCTGAGA+AGG 0.597067 5.1:+1045632 MS.gene92305:CDS
AAGAATTCTATGTGATCACA+TGG 0.598132 5.1:+1045415 MS.gene92305:CDS
AACCCTGGTCGTGAAGGAGT+TGG 0.600436 5.1:+1045657 MS.gene92305:CDS
CTTCCTCCAAAGATTGGTGA+AGG 0.600903 5.1:-1045360 None:intergenic
ATTATGGCTGCACCACCAGT+TGG 0.609110 5.1:-1045468 None:intergenic
GACCAACTCCTTCACGACCA+GGG 0.644619 5.1:-1045659 None:intergenic
GGTGCAGCCATAATGAGAGA+AGG 0.648728 5.1:+1045477 MS.gene92305:CDS
TACCCTGAGAAGGTTAACCC+TGG 0.651425 5.1:+1045642 MS.gene92305:CDS
ATTCATCAACAACATGGCAA+TGG 0.658103 5.1:+1045113 None:intergenic
GACCAGGGTTAACCTTCTCA+GGG 0.658939 5.1:-1045644 None:intergenic
ATCTTTGAGATGCCAACTGG+TGG 0.674480 5.1:+1045456 MS.gene92305:CDS
AGAATTCTATGTGATCACAT+GGG 0.727784 5.1:+1045416 MS.gene92305:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAGTAAACTTAACTTCAATT+GGG - chr5.1:1045716-1045735 None:intergenic 25.0%
!! AGATTAAGTATCAGTTTTAC+AGG + chr5.1:1045571-1045590 MS.gene92305:CDS 25.0%
!! GATTAAGTATCAGTTTTACA+GGG + chr5.1:1045572-1045591 MS.gene92305:CDS 25.0%
AAATATTGAACCTCACCATT+AGG - chr5.1:1045603-1045622 None:intergenic 30.0%
AAGAATTCTATGTGATCACA+TGG + chr5.1:1045415-1045434 MS.gene92305:CDS 30.0%
AATATTGAACCTCACCATTA+GGG - chr5.1:1045602-1045621 None:intergenic 30.0%
AGAATTCTATGTGATCACAT+GGG + chr5.1:1045416-1045435 MS.gene92305:CDS 30.0%
CCAATTGAAGTTAAGTTTAC+TGG + chr5.1:1045714-1045733 MS.gene92305:CDS 30.0%
CCAGTAAACTTAACTTCAAT+TGG - chr5.1:1045717-1045736 None:intergenic 30.0%
! ATCTCAAAGATTTGTTCCTT+AGG - chr5.1:1045447-1045466 None:intergenic 30.0%
!! TGGCTTTAGGAAATAGATTA+AGG + chr5.1:1045538-1045557 MS.gene92305:CDS 30.0%
CAATATTTGCATCCTAAGGA+TGG + chr5.1:1045615-1045634 MS.gene92305:CDS 35.0%
CTAACCTTCTTAAGTTAGCA+AGG + chr5.1:1045502-1045521 MS.gene92305:CDS 35.0%
CTTGCTAACTTAAGAAGGTT+AGG - chr5.1:1045504-1045523 None:intergenic 35.0%
CTTTCCTTGCTAACTTAAGA+AGG - chr5.1:1045509-1045528 None:intergenic 35.0%
GAGATGAAGGATAAAGTTGA+TGG - chr5.1:1045195-1045214 None:intergenic 35.0%
GGTTCAATATTTGCATCCTA+AGG + chr5.1:1045611-1045630 MS.gene92305:CDS 35.0%
! ATGGTTGTTTCCATGGTTTA+GGG - chr5.1:1045176-1045195 None:intergenic 35.0%
! GTTCTTGTTGTGAACTTGAT+GGG - chr5.1:1045225-1045244 None:intergenic 35.0%
! TGTTCTTGTTGTGAACTTGA+TGG - chr5.1:1045226-1045245 None:intergenic 35.0%
! TTTTACAGGGTTTTCCCTAA+TGG + chr5.1:1045585-1045604 MS.gene92305:CDS 35.0%
!! AAAGAACAGTGTTTGGCTTT+AGG + chr5.1:1045525-1045544 MS.gene92305:CDS 35.0%
!! AAAGTTGATGGTTGTTTCCA+TGG - chr5.1:1045183-1045202 None:intergenic 35.0%
AACACACCTTCACCAATCTT+TGG + chr5.1:1045354-1045373 MS.gene92305:CDS 40.0%
AGCAAGGAAAGAACAGTGTT+TGG + chr5.1:1045518-1045537 MS.gene92305:CDS 40.0%
GTTAGGACCTTCTCTCATTA+TGG - chr5.1:1045487-1045506 None:intergenic 40.0%
! AAGATTGGTGAAGGTGTGTT+TGG - chr5.1:1045354-1045373 None:intergenic 40.0%
! AAGGAGTTGGTCAGAATTTC+AGG + chr5.1:1045670-1045689 MS.gene92305:CDS 40.0%
! AGATTGGTGAAGGTGTGTTT+GGG - chr5.1:1045353-1045372 None:intergenic 40.0%
! GATGGTTGTTTCCATGGTTT+AGG - chr5.1:1045177-1045196 None:intergenic 40.0%
! GCTTCTTCTTTTGTTGCAAC+TGG - chr5.1:1045291-1045310 None:intergenic 40.0%
! GGTCAGAATTTCAGGTCTAT+TGG + chr5.1:1045678-1045697 MS.gene92305:CDS 40.0%
!! CAAATCTTTGAGATGCCAAC+TGG + chr5.1:1045453-1045472 MS.gene92305:CDS 40.0%
ACACCTTCACCAATCTTTGG+AGG + chr5.1:1045357-1045376 MS.gene92305:CDS 45.0%
CTTCCTCCAAAGATTGGTGA+AGG - chr5.1:1045363-1045382 None:intergenic 45.0%
! GATGGGTTTGAGAGAGATGA+AGG - chr5.1:1045208-1045227 None:intergenic 45.0%
!! ATCTTTGAGATGCCAACTGG+TGG + chr5.1:1045456-1045475 MS.gene92305:CDS 45.0%
!! CACTGGTGGTTTGTTACGTA+AGG + chr5.1:1045383-1045402 MS.gene92305:CDS 45.0%
AAGGTTAACCCTGGTCGTGA+AGG + chr5.1:1045651-1045670 MS.gene92305:CDS 50.0%
ATTATGGCTGCACCACCAGT+TGG - chr5.1:1045471-1045490 None:intergenic 50.0%
CCAGTGCTTCCTCCAAAGAT+TGG - chr5.1:1045369-1045388 None:intergenic 50.0%
CTCTCTCAAGCCCTAAACCA+TGG + chr5.1:1045163-1045182 MS.gene92305:CDS 50.0%
GACCAGGGTTAACCTTCTCA+GGG - chr5.1:1045647-1045666 None:intergenic 50.0%
GATCACATGGGAGTCACCTA+AGG + chr5.1:1045428-1045447 MS.gene92305:CDS 50.0%
GGTGCAGCCATAATGAGAGA+AGG + chr5.1:1045477-1045496 MS.gene92305:CDS 50.0%
TACCCTGAGAAGGTTAACCC+TGG + chr5.1:1045642-1045661 MS.gene92305:CDS 50.0%
TCAGGGTACACACCATCCTT+AGG - chr5.1:1045630-1045649 None:intergenic 50.0%
TCAGGTGGAGTGAAACCAAC+TGG - chr5.1:1045324-1045343 None:intergenic 50.0%
! CAGGGTTTTCCCTAATGGTG+AGG + chr5.1:1045590-1045609 MS.gene92305:CDS 50.0%
! CATGGTTTAGGGCTTGAGAG+AGG - chr5.1:1045165-1045184 None:intergenic 50.0%
! CCAATCTTTGGAGGAAGCAC+TGG + chr5.1:1045366-1045385 MS.gene92305:CDS 50.0%
! GGTGTGTTTGGGTCTAGTTC+AGG - chr5.1:1045342-1045361 None:intergenic 50.0%
! GTGTTTGGGTCTAGTTCAGG+TGG - chr5.1:1045339-1045358 None:intergenic 50.0%
!! ATCTTTGGAGGAAGCACTGG+TGG + chr5.1:1045369-1045388 MS.gene92305:CDS 50.0%
AACCCTGGTCGTGAAGGAGT+TGG + chr5.1:1045657-1045676 MS.gene92305:CDS 55.0%
CGACCAGGGTTAACCTTCTC+AGG - chr5.1:1045648-1045667 None:intergenic 55.0%
GACCAACTCCTTCACGACCA+GGG - chr5.1:1045662-1045681 None:intergenic 55.0%
GAGAGGTGGAGTGAAGAGAG+AGG - chr5.1:1045148-1045167 None:intergenic 55.0%
GGATGGTGTGTACCCTGAGA+AGG + chr5.1:1045632-1045651 MS.gene92305:CDS 55.0%
GTGAAACCAACTGGTGCAGC+AGG - chr5.1:1045315-1045334 None:intergenic 55.0%
TGACCAACTCCTTCACGACC+AGG - chr5.1:1045663-1045682 None:intergenic 55.0%
! GGTTTAGGGCTTGAGAGAGG+TGG - chr5.1:1045162-1045181 None:intergenic 55.0%
GAAGCTCCTGCTGCACCAGT+TGG + chr5.1:1045306-1045325 MS.gene92305:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 1045126 1045758 1045126 ID=MS.gene92305
chr5.1 mRNA 1045126 1045758 1045126 ID=MS.gene92305.t1;Parent=MS.gene92305
chr5.1 exon 1045126 1045758 1045126 ID=MS.gene92305.t1.exon1;Parent=MS.gene92305.t1
chr5.1 CDS 1045126 1045758 1045126 ID=cds.MS.gene92305.t1;Parent=MS.gene92305.t1
Gene Sequence

>MS.gene92305

ATGGCAATGGCAACTCAAGCCTCTCTCTTCACTCCACCTCTCTCAAGCCCTAAACCATGGAAACAACCATCAACTTTATCCTTCATCTCTCTCAAACCCATCAAGTTCACAACAAGAACAACAAAAATCTCAGCTGCAGATGACAAAACAGAAGCAGCAGCACCAGTTGCAACAAAAGAAGAAGCTCCTGCTGCACCAGTTGGTTTCACTCCACCTGAACTAGACCCAAACACACCTTCACCAATCTTTGGAGGAAGCACTGGTGGTTTGTTACGTAAGGCACAAGTTGAAGAATTCTATGTGATCACATGGGAGTCACCTAAGGAACAAATCTTTGAGATGCCAACTGGTGGTGCAGCCATAATGAGAGAAGGTCCTAACCTTCTTAAGTTAGCAAGGAAAGAACAGTGTTTGGCTTTAGGAAATAGATTAAGGTCTAAGTACAAGATTAAGTATCAGTTTTACAGGGTTTTCCCTAATGGTGAGGTTCAATATTTGCATCCTAAGGATGGTGTGTACCCTGAGAAGGTTAACCCTGGTCGTGAAGGAGTTGGTCAGAATTTCAGGTCTATTGGAAAGAATGTTAGCCCAATTGAAGTTAAGTTTACTGGTAAGCAACCTTTTGATGTGTAA

Protein sequence

>MS.gene92305.t1

MAMATQASLFTPPLSSPKPWKQPSTLSFISLKPIKFTTRTTKISAADDKTEAAAPVATKEEAPAAPVGFTPPELDPNTPSPIFGGSTGGLLRKAQVEEFYVITWESPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGNRLRSKYKIKYQFYRVFPNGEVQYLHPKDGVYPEKVNPGREGVGQNFRSIGKNVSPIEVKFTGKQPFDV