AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90216


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90216.t1 MTR_4g093560 98.864 176 2 0 1 176 1 176 1.58e-131 365
MS.gene90216.t1 MTR_2g009600 67.630 173 55 1 2 173 3 175 2.94e-86 252
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90216.t1 AT2G14110 66.860 172 56 1 5 175 4 175 2.87e-86 251
MS.gene90216.t1 AT2G14110 68.421 133 42 0 5 137 4 136 4.96e-69 206

Find 35 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAATAACAGGTTTCAAATT+TGG 0.132575 4.2:+19839067 MS.gene90216:intron
TTTGGGAGTTACAAGTATTT+TGG 0.197480 4.2:+19839085 MS.gene90216:CDS
CAATAACAGGTTTCAAATTT+GGG 0.308384 4.2:+19839068 MS.gene90216:intron
GTTAACAATGGCTTAAATCT+AGG 0.314102 4.2:+19839107 MS.gene90216:CDS
TCGATTTAGATTATACTCTT+TGG 0.338666 4.2:+19838407 MS.gene90216:CDS
CAGAAAATTCATTCAAAGAC+CGG 0.400088 4.2:+19838883 MS.gene90216:CDS
CACCTCAATGTTTGTCGCCC+AGG 0.413707 4.2:+19838693 MS.gene90216:CDS
GAAAGAAGAGATGGAGGAGG+TGG 0.415362 4.2:+19838318 None:intergenic
ATACTCTTTGGCCTTTCTAC+TGG 0.426381 4.2:+19838419 MS.gene90216:CDS
AGAGATGGAGGAGGTGGAGA+AGG 0.429450 4.2:+19838324 None:intergenic
GCTGAGGCAGTGCAAATGAT+AGG 0.438625 4.2:+19838352 MS.gene90216:CDS
CCTCAAAGAACAAGCAGAAA+AGG 0.469406 4.2:+19839174 MS.gene90216:CDS
TGATAACAATAACATGAAAC+CGG 0.472254 4.2:+19838939 MS.gene90216:CDS
GAGTGCACTCAAGATGCCTC+TGG 0.487612 4.2:-19838587 None:intergenic
CAAAGAAGGTGTGTCGTGCT+TGG 0.497441 4.2:-19838554 None:intergenic
TATGTGTGGAGTTGGTGATT+TGG 0.498178 4.2:-19838641 None:intergenic
AGAGAAAGAAGAGATGGAGG+AGG 0.524195 4.2:+19838315 None:intergenic
CTCAAGATGCCTCTGGAATG+TGG 0.524198 4.2:-19838580 None:intergenic
TTGAGTGCACTCAAAGATGA+AGG 0.526991 4.2:+19838598 MS.gene90216:CDS
AAATCGAACACTACGAGCCT+TGG 0.527042 4.2:-19838391 None:intergenic
AATCTAGGAGTATTTAGAGA+AGG 0.528974 4.2:+19839122 MS.gene90216:CDS
GTACAAATTTACCAGTAGAA+AGG 0.530754 4.2:-19838430 None:intergenic
GGAGGAGGTGGAGAAGGTGA+AGG 0.537062 4.2:+19838330 MS.gene90216:CDS
ATCTAGGAGTATTTAGAGAA+GGG 0.541922 4.2:+19839123 MS.gene90216:CDS
GGTGGAGAAGGTGAAGGCTG+AGG 0.552861 4.2:+19838336 MS.gene90216:CDS
CTGGAATGTGGAAACAAAGA+AGG 0.568363 4.2:-19838568 None:intergenic
GTTGTTGCTATGTGTGGAGT+TGG 0.571433 4.2:-19838649 None:intergenic
CATAGAGTTGTAGGGGACAC+CGG 0.577518 4.2:-19838902 None:intergenic
AGTTGGTGATTTGGAAGCAA+TGG 0.603835 4.2:-19838632 None:intergenic
AGATACGTTGTTGCTATGTG+TGG 0.622048 4.2:-19838655 None:intergenic
AACCTGGGCGACAAACATTG+AGG 0.630045 4.2:-19838695 None:intergenic
TCTTTGTTTCCACATTCCAG+AGG 0.646314 4.2:+19838571 MS.gene90216:CDS
AAAAGAGCATAGAGTTGTAG+GGG 0.649290 4.2:-19838909 None:intergenic
AAGGTGTGTCGTGCTTGGAG+CGG 0.652306 4.2:-19838549 None:intergenic
GAATGTTTCAAGTACTACCA+AGG 0.677669 4.2:+19838374 MS.gene90216:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGAGAAATTAAATTT+GGG - chr4.2:19838815-19838834 None:intergenic 10.0%
!! TAAAAAAGAGAAATTAAATT+TGG - chr4.2:19838816-19838835 None:intergenic 10.0%
!!! TTGTATGTATATTTATTTTA+CGG + chr4.2:19838774-19838793 MS.gene90216:intron 10.0%
!! AACAAAATAAAATGATATAC+CGG - chr4.2:19838961-19838980 None:intergenic 15.0%
!!! ATTTCTCTTTTTTATTGTTT+AGG + chr4.2:19838822-19838841 MS.gene90216:intron 15.0%
! AAATAAGCGCAATAAAAAAG+CGG - chr4.2:19838756-19838775 None:intergenic 25.0%
! CAATAACAGGTTTCAAATTT+GGG + chr4.2:19839068-19839087 MS.gene90216:intron 25.0%
! TCGATTTAGATTATACTCTT+TGG + chr4.2:19838407-19838426 MS.gene90216:CDS 25.0%
! TGATAACAATAACATGAAAC+CGG + chr4.2:19838939-19838958 MS.gene90216:CDS 25.0%
!! AAATTCTCTTATTTGTTTGG+AGG + chr4.2:19839001-19839020 MS.gene90216:intron 25.0%
!! CTCAAATTCTCTTATTTGTT+TGG + chr4.2:19838998-19839017 MS.gene90216:intron 25.0%
!! TTTTCATCTTTCCTATTTTC+TGG + chr4.2:19838463-19838482 MS.gene90216:intron 25.0%
!!! ACAAGTATTTTGGTTAACAA+TGG + chr4.2:19839095-19839114 MS.gene90216:CDS 25.0%
CAGAAAATTCATTCAAAGAC+CGG + chr4.2:19838883-19838902 MS.gene90216:CDS 30.0%
CCAATAACAGGTTTCAAATT+TGG + chr4.2:19839067-19839086 MS.gene90216:intron 30.0%
GTACAAATTTACCAGTAGAA+AGG - chr4.2:19838433-19838452 None:intergenic 30.0%
GTTAACAATGGCTTAAATCT+AGG + chr4.2:19839107-19839126 MS.gene90216:CDS 30.0%
! AAAAAGAGCATAGAGTTGTA+GGG - chr4.2:19838913-19838932 None:intergenic 30.0%
! CCAAATTTGAAACCTGTTAT+TGG - chr4.2:19839070-19839089 None:intergenic 30.0%
! TTTGGGAGTTACAAGTATTT+TGG + chr4.2:19839085-19839104 MS.gene90216:CDS 30.0%
!! AATCTAGGAGTATTTAGAGA+AGG + chr4.2:19839122-19839141 MS.gene90216:CDS 30.0%
!! ATCTAGGAGTATTTAGAGAA+GGG + chr4.2:19839123-19839142 MS.gene90216:CDS 30.0%
TAGTGAGTACTCCAGAAAAT+AGG - chr4.2:19838477-19838496 None:intergenic 35.0%
! AAAAGAGCATAGAGTTGTAG+GGG - chr4.2:19838912-19838931 None:intergenic 35.0%
! CACAAGATTTTCGCAAAACT+GGG + chr4.2:19839148-19839167 MS.gene90216:CDS 35.0%
! GAAAAAGAGCATAGAGTTGT+AGG - chr4.2:19838914-19838933 None:intergenic 35.0%
! GAATGTTTCAAGTACTACCA+AGG + chr4.2:19838374-19838393 MS.gene90216:CDS 35.0%
! TCACAAGATTTTCGCAAAAC+TGG + chr4.2:19839147-19839166 MS.gene90216:CDS 35.0%
!! TGATTTGTTGTGCCAATAAC+AGG + chr4.2:19839055-19839074 MS.gene90216:intron 35.0%
ACTGAGGAACAAACAAACCT+GGG - chr4.2:19838713-19838732 None:intergenic 40.0%
AGATACGTTGTTGCTATGTG+TGG - chr4.2:19838658-19838677 None:intergenic 40.0%
CCTCAAAGAACAAGCAGAAA+AGG + chr4.2:19839174-19839193 MS.gene90216:CDS 40.0%
CTGGAATGTGGAAACAAAGA+AGG - chr4.2:19838571-19838590 None:intergenic 40.0%
TTGAGTGCACTCAAAGATGA+AGG + chr4.2:19838598-19838617 MS.gene90216:CDS 40.0%
! ATACTCTTTGGCCTTTCTAC+TGG + chr4.2:19838419-19838438 MS.gene90216:CDS 40.0%
! CCTTTTCTGCTTGTTCTTTG+AGG - chr4.2:19839177-19839196 None:intergenic 40.0%
! TCTTTGTTTCCACATTCCAG+AGG + chr4.2:19838571-19838590 MS.gene90216:CDS 40.0%
!! AGTTGGTGATTTGGAAGCAA+TGG - chr4.2:19838635-19838654 None:intergenic 40.0%
!! TATGTGTGGAGTTGGTGATT+TGG - chr4.2:19838644-19838663 None:intergenic 40.0%
AAATCGAACACTACGAGCCT+TGG - chr4.2:19838394-19838413 None:intergenic 45.0%
AAGTGTTCGTCTTTCCACTG+AGG - chr4.2:19838729-19838748 None:intergenic 45.0%
CACTGAGGAACAAACAAACC+TGG - chr4.2:19838714-19838733 None:intergenic 45.0%
GTTGTTGCTATGTGTGGAGT+TGG - chr4.2:19838652-19838671 None:intergenic 45.0%
! CAGGTTTGTTTGTTCCTCAG+TGG + chr4.2:19838712-19838731 MS.gene90216:intron 45.0%
AACCTGGGCGACAAACATTG+AGG - chr4.2:19838698-19838717 None:intergenic 50.0%
CATAGAGTTGTAGGGGACAC+CGG - chr4.2:19838905-19838924 None:intergenic 50.0%
GCTGAGGCAGTGCAAATGAT+AGG + chr4.2:19838352-19838371 MS.gene90216:CDS 50.0%
! CAAAGAAGGTGTGTCGTGCT+TGG - chr4.2:19838557-19838576 None:intergenic 50.0%
!! CTCAAGATGCCTCTGGAATG+TGG - chr4.2:19838583-19838602 None:intergenic 50.0%
AAGGTGTGTCGTGCTTGGAG+CGG - chr4.2:19838552-19838571 None:intergenic 55.0%
CACCTCAATGTTTGTCGCCC+AGG + chr4.2:19838693-19838712 MS.gene90216:CDS 55.0%
GAGTGCACTCAAGATGCCTC+TGG - chr4.2:19838590-19838609 None:intergenic 55.0%
GGAGGAGGTGGAGAAGGTGA+AGG + chr4.2:19838330-19838349 MS.gene90216:CDS 60.0%
GGTGGAGAAGGTGAAGGCTG+AGG + chr4.2:19838336-19838355 MS.gene90216:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 19838328 19839205 19838328 ID=MS.gene90216
chr4.2 mRNA 19838328 19839205 19838328 ID=MS.gene90216.t1;Parent=MS.gene90216
chr4.2 exon 19838328 19838440 19838328 ID=MS.gene90216.t1.exon1;Parent=MS.gene90216.t1
chr4.2 CDS 19838328 19838440 19838328 ID=cds.MS.gene90216.t1;Parent=MS.gene90216.t1
chr4.2 exon 19838543 19838714 19838543 ID=MS.gene90216.t1.exon2;Parent=MS.gene90216.t1
chr4.2 CDS 19838543 19838714 19838543 ID=cds.MS.gene90216.t1;Parent=MS.gene90216.t1
chr4.2 exon 19838844 19838960 19838844 ID=MS.gene90216.t1.exon3;Parent=MS.gene90216.t1
chr4.2 CDS 19838844 19838960 19838844 ID=cds.MS.gene90216.t1;Parent=MS.gene90216.t1
chr4.2 exon 19839077 19839205 19839077 ID=MS.gene90216.t1.exon4;Parent=MS.gene90216.t1
chr4.2 CDS 19839077 19839205 19839077 ID=cds.MS.gene90216.t1;Parent=MS.gene90216.t1
Gene Sequence

>MS.gene90216

ATGGAGGAGGTGGAGAAGGTGAAGGCTGAGGCAGTGCAAATGATAGGAATGTTTCAAGTACTACCAAGGCTCGTAGTGTTCGATTTAGATTATACTCTTTGGCCTTTCTACTGGTAAATTTGTACATGATACATCTTTTCATCTTTCCTATTTTCTGGAGTACTCACTAAATATTCTTTCTTATTTTACTACACAAAATGAATCTCAAATTTCAGTGAGTGCCGCTCCAAGCACGACACACCTTCTTTGTTTCCACATTCCAGAGGCATCTTGAGTGCACTCAAAGATGAAGGAATTGATGCTGCCATTGCTTCCAAATCACCAACTCCACACATAGCAACAACGTATCTTGACAAACTCAAAATCACCTCAATGTTTGTCGCCCAGGTTTGTTTGTTCCTCAGTGGAAAGACGAACACTTCTGTCCGCTTTTTTATTGCGCTTATTTGTATGTATATTTATTTTACGGCACGTTCTTGTTAGACCCAAATTTAATTTCTCTTTTTTATTGTTTAGGAAATATTTTACAGCTCGACACACAAAACAGAACATTTTCAGAAAATTCATTCAAAGACCGGTGTCCCCTACAACTCTATGCTCTTTTTCGACGATGATAACAATAACATGAAACCGGTATATCATTTTATTTTGTTTTTTAATTAATTAAGTTCTCAAATTCTCTTATTTGTTTGGAGGAGTGATTATCAATTTGTCATATCATGTAACATGATTTGTTGTGCCAATAACAGGTTTCAAATTTGGGAGTTACAAGTATTTTGGTTAACAATGGCTTAAATCTAGGAGTATTTAGAGAAGGGCTCACAAGATTTTCGCAAAACTGGGATGCCTCAAAGAACAAGCAGAAAAGGCGCAAGTAA

Protein sequence

>MS.gene90216.t1

MEEVEKVKAEAVQMIGMFQVLPRLVVFDLDYTLWPFYCECRSKHDTPSLFPHSRGILSALKDEGIDAAIASKSPTPHIATTYLDKLKITSMFVAQEIFYSSTHKTEHFQKIHSKTGVPYNSMLFFDDDNNNMKPVSNLGVTSILVNNGLNLGVFREGLTRFSQNWDASKNKQKRRK