AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25856


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25856.t1 MTR_3g079520 94.009 217 13 0 1 217 1 217 8.36e-147 408
MS.gene25856.t1 MTR_3g479600 94.009 217 13 0 1 217 1 217 8.36e-147 408
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25856.t1 AT2G21530 59.231 130 52 1 82 211 81 209 9.38e-51 164

Find 40 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCTTCTTGTTATAGATT+TGG 0.263843 3.2:-69956417 MS.gene25856:CDS
TCTGGGAGTTATATTACTTT+TGG 0.301923 3.2:-69956329 MS.gene25856:intron
AATGCCAGGGGACACTAATT+TGG 0.313943 3.2:-69956102 MS.gene25856:intron
ACTTCTATACCTGTTGCAAC+TGG 0.344018 3.2:+69956634 None:intergenic
CCATTGTCTAGTTCACCTTC+TGG 0.345009 3.2:+69956026 None:intergenic
GCAGAACATGTTTGAGACTT+TGG 0.358567 3.2:+69957310 None:intergenic
GTAGTTACCACTGTATCTTC+TGG 0.366490 3.2:-69956347 MS.gene25856:CDS
CTTTGTGTAGCATTAGTTGT+TGG 0.401469 3.2:+69957112 None:intergenic
GCATTGGAGTGTAATAAAAT+TGG 0.429187 3.2:+69957271 None:intergenic
ATCACTAAATCAGCTTTCTC+TGG 0.443107 3.2:+69956658 None:intergenic
TTGATGCATGTATAGCTCCT+AGG 0.449624 3.2:+69957146 None:intergenic
TTAGTGATCCCAGTTGCAAC+AGG 0.462387 3.2:-69956643 MS.gene25856:intron
TTCAAGGCATATAGGCTTCA+AGG 0.462486 3.2:-69956813 MS.gene25856:CDS
GATGGTGATTCAAGGCATAT+AGG 0.467110 3.2:-69956821 MS.gene25856:CDS
AGCACCAATGGAACATTCAT+TGG 0.469041 3.2:-69956392 MS.gene25856:CDS
GAACATTCATTGGTGACAAA+AGG 0.471831 3.2:-69956382 MS.gene25856:CDS
TAGTTACCACTGTATCTTCT+GGG 0.476442 3.2:-69956346 MS.gene25856:CDS
CAACTAATGCTACACAAAGA+TGG 0.490326 3.2:-69957108 MS.gene25856:CDS
TGATAGGAGATGGTGATTCA+AGG 0.491582 3.2:-69956829 MS.gene25856:intron
GAAAGTTACTGAAGTGGCAT+TGG 0.511446 3.2:+69957255 None:intergenic
GTCACCAATGAATGTTCCAT+TGG 0.522297 3.2:+69956388 None:intergenic
GAGAAGGCTGCAGATACAGT+TGG 0.528528 3.2:-69956053 MS.gene25856:CDS
GGTGACAAAAGGCTGAAACC+TGG 0.545814 3.2:-69956371 MS.gene25856:CDS
AGATGGATTCTTGAACCAGT+TGG 0.550406 3.2:-69957091 MS.gene25856:intron
ATAGATTTGGATAGCACCAA+TGG 0.560750 3.2:-69956404 MS.gene25856:CDS
GGCTTCAAGGTTGAGAGACC+AGG 0.565164 3.2:-69956800 MS.gene25856:CDS
GATACAGTGGTAACTACTCC+AGG 0.567838 3.2:+69956353 None:intergenic
TGTTATACATCCCAAATCAC+AGG 0.568456 3.2:-69957179 MS.gene25856:CDS
CTTCTATACCTGTTGCAACT+GGG 0.577256 3.2:+69956635 None:intergenic
CAGGTGTGCATGCACGCATT+CGG 0.581165 3.2:-69956451 MS.gene25856:CDS
TGTGTCGAAAATAGAAGAGA+AGG 0.593642 3.2:-69956069 MS.gene25856:CDS
TGTAGCATTAGTTGTTGGAG+TGG 0.594055 3.2:+69957117 None:intergenic
ACTCTAGCAACAATAATGGA+AGG 0.614859 3.2:-69957364 None:intergenic
ATCTAGAATGAAGTCACTGT+AGG 0.636581 3.2:-69956688 MS.gene25856:intron
TGAACTGAAAGTTACTGAAG+TGG 0.650521 3.2:+69957249 None:intergenic
GATACAGTTGGAGAACCAGA+AGG 0.670779 3.2:-69956041 MS.gene25856:CDS
GAAGTAATTTCATAAGCACC+TGG 0.674584 3.2:+69956782 None:intergenic
TTGGGATGTATAACACGACC+TGG 0.687791 3.2:+69957187 None:intergenic
ATAACTCCCAGAAGATACAG+TGG 0.690459 3.2:+69956340 None:intergenic
CCAGAAGGTGAACTAGACAA+TGG 0.701962 3.2:-69956026 MS.gene25856:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAGTATTTGTTTCAATA+TGG - chr3.2:69956431-69956450 MS.gene25856:CDS 15.0%
!!! GATTTTAACAATTTTTCAAT+TGG + chr3.2:69956302-69956321 None:intergenic 15.0%
!! ACATAATTATTCAACAGAAA+AGG + chr3.2:69957066-69957085 None:intergenic 20.0%
!! TATTTGTAGTAATATCATGA+AGG + chr3.2:69956389-69956408 None:intergenic 20.0%
!! TGAAACAAATACTTAAAAAG+TGG + chr3.2:69956428-69956447 None:intergenic 20.0%
!! TTCATGATATTACTACAAAT+AGG - chr3.2:69956388-69956407 MS.gene25856:CDS 20.0%
!! TTTGTAACTGTAAGAAAAAT+AGG + chr3.2:69956755-69956774 None:intergenic 20.0%
!!! TATCATTTTGAAATTCTCTT+AGG + chr3.2:69957158-69957177 None:intergenic 20.0%
! ACTTAAAAAGTGGTTAATTG+AGG + chr3.2:69956418-69956437 None:intergenic 25.0%
! AGCATAGTACATTAAGATTA+AGG + chr3.2:69956801-69956820 None:intergenic 25.0%
! ATAATAGCAGAGGTTAAATA+TGG + chr3.2:69957209-69957228 None:intergenic 25.0%
! CAAATTATATTGTGATTCTC+TGG + chr3.2:69956835-69956854 None:intergenic 25.0%
! CAATATGGTCAATATTAACA+AGG - chr3.2:69956446-69956465 MS.gene25856:CDS 25.0%
! CTCAATCAAGATAAGAAAAA+TGG + chr3.2:69956348-69956367 None:intergenic 25.0%
! CTTAAAAAGTGGTTAATTGA+GGG + chr3.2:69956417-69956436 None:intergenic 25.0%
! TTGAAATTCTCTTAGGAATA+TGG + chr3.2:69957151-69957170 None:intergenic 25.0%
!! CAATCTTCTTGTTATAGATT+TGG - chr3.2:69956915-69956934 MS.gene25856:intron 25.0%
!!! ACAAAACCAATCATTTTGAT+AGG - chr3.2:69956487-69956506 MS.gene25856:intron 25.0%
CAGAGGTTAAATATGGAATA+GGG + chr3.2:69957202-69957221 None:intergenic 30.0%
GCATTGGAGTGTAATAAAAT+TGG + chr3.2:69956064-69956083 None:intergenic 30.0%
TATATAACCCTGTTTGTAGA+AGG - chr3.2:69957112-69957131 MS.gene25856:CDS 30.0%
TTAAGATCCTTCTACAAACA+GGG + chr3.2:69957122-69957141 None:intergenic 30.0%
! ATCAGAATCAATGATTTGTG+TGG - chr3.2:69956583-69956602 MS.gene25856:intron 30.0%
! TCTGGGAGTTATATTACTTT+TGG - chr3.2:69957003-69957022 MS.gene25856:intron 30.0%
! TGTTTTGTATTTACAGTGTC+AGG - chr3.2:69956862-69956881 MS.gene25856:intron 30.0%
! TTTAAGATCCTTCTACAAAC+AGG + chr3.2:69957123-69957142 None:intergenic 30.0%
!! AACAAGGTGTTTTATCACTT+TGG - chr3.2:69956462-69956481 MS.gene25856:intron 30.0%
!!! GAGACTTTGGAATTTTTGTT+AGG + chr3.2:69956012-69956031 None:intergenic 30.0%
!!! TTCACTTTTTCCTGTGATTT+GGG + chr3.2:69956166-69956185 None:intergenic 30.0%
ATAGATTTGGATAGCACCAA+TGG - chr3.2:69956928-69956947 MS.gene25856:intron 35.0%
ATCACTAAATCAGCTTTCTC+TGG + chr3.2:69956677-69956696 None:intergenic 35.0%
CAACTAATGCTACACAAAGA+TGG - chr3.2:69956224-69956243 MS.gene25856:intron 35.0%
CCATCTCCTATCAAAATGAT+TGG + chr3.2:69956496-69956515 None:intergenic 35.0%
CTTTGTGTAGCATTAGTTGT+TGG + chr3.2:69956223-69956242 None:intergenic 35.0%
GAAGTAATTTCATAAGCACC+TGG + chr3.2:69956553-69956572 None:intergenic 35.0%
GATTAAGGAGCTTATTTCAG+TGG + chr3.2:69956786-69956805 None:intergenic 35.0%
GCAGAGGTTAAATATGGAAT+AGG + chr3.2:69957203-69957222 None:intergenic 35.0%
TAGTTACCACTGTATCTTCT+GGG - chr3.2:69956986-69957005 MS.gene25856:intron 35.0%
TGAACTGAAAGTTACTGAAG+TGG + chr3.2:69956086-69956105 None:intergenic 35.0%
TGTGTCGAAAATAGAAGAGA+AGG - chr3.2:69957263-69957282 MS.gene25856:CDS 35.0%
TGTTATACATCCCAAATCAC+AGG - chr3.2:69956153-69956172 MS.gene25856:intron 35.0%
! AGAAAGTGTAACTAACCAAC+TGG + chr3.2:69956259-69956278 None:intergenic 35.0%
! ATCTAGAATGAAGTCACTGT+AGG - chr3.2:69956644-69956663 MS.gene25856:CDS 35.0%
! GAACATTCATTGGTGACAAA+AGG - chr3.2:69956950-69956969 MS.gene25856:intron 35.0%
! TCTCTTCTATTTTCGACACA+CGG + chr3.2:69957263-69957282 None:intergenic 35.0%
!! CCAATCATTTTGATAGGAGA+TGG - chr3.2:69956493-69956512 MS.gene25856:intron 35.0%
!!! GTTCACTTTTTCCTGTGATT+TGG + chr3.2:69956167-69956186 None:intergenic 35.0%
ACTTCTATACCTGTTGCAAC+TGG + chr3.2:69956701-69956720 None:intergenic 40.0%
AGCACCAATGGAACATTCAT+TGG - chr3.2:69956940-69956959 MS.gene25856:intron 40.0%
ATAACTCCCAGAAGATACAG+TGG + chr3.2:69956995-69957014 None:intergenic 40.0%
CTCTGCTATTATGAATGCCA+GGG - chr3.2:69957217-69957236 MS.gene25856:CDS 40.0%
CTTCTATACCTGTTGCAACT+GGG + chr3.2:69956700-69956719 None:intergenic 40.0%
GAAAGTTACTGAAGTGGCAT+TGG + chr3.2:69956080-69956099 None:intergenic 40.0%
GATGGTGATTCAAGGCATAT+AGG - chr3.2:69956511-69956530 MS.gene25856:intron 40.0%
GTAGTTACCACTGTATCTTC+TGG - chr3.2:69956985-69957004 MS.gene25856:intron 40.0%
GTCACCAATGAATGTTCCAT+TGG + chr3.2:69956947-69956966 None:intergenic 40.0%
TCACAGGAAAAAGTGAACCT+AGG - chr3.2:69956169-69956188 MS.gene25856:intron 40.0%
TCTGCTATTATGAATGCCAG+GGG - chr3.2:69957218-69957237 MS.gene25856:CDS 40.0%
TGATAGGAGATGGTGATTCA+AGG - chr3.2:69956503-69956522 MS.gene25856:intron 40.0%
TGTAGCATTAGTTGTTGGAG+TGG + chr3.2:69956218-69956237 None:intergenic 40.0%
TTCAAGGCATATAGGCTTCA+AGG - chr3.2:69956519-69956538 MS.gene25856:intron 40.0%
TTGATGCATGTATAGCTCCT+AGG + chr3.2:69956189-69956208 None:intergenic 40.0%
! AGATGGATTCTTGAACCAGT+TGG - chr3.2:69956241-69956260 MS.gene25856:intron 40.0%
! GCAGAACATGTTTGAGACTT+TGG + chr3.2:69956025-69956044 None:intergenic 40.0%
!!! TGCTTTCTTTTTCAGCAACC+AGG - chr3.2:69956127-69956146 MS.gene25856:intron 40.0%
AATGCCAGGGGACACTAATT+TGG - chr3.2:69957230-69957249 MS.gene25856:CDS 45.0%
ATAGCCAAATTAGTGTCCCC+TGG + chr3.2:69957237-69957256 None:intergenic 45.0%
CCAGAAGGTGAACTAGACAA+TGG - chr3.2:69957306-69957325 MS.gene25856:CDS 45.0%
CCTCTGCTATTATGAATGCC+AGG - chr3.2:69957216-69957235 MS.gene25856:CDS 45.0%
CCTGGCATTCATAATAGCAG+AGG + chr3.2:69957219-69957238 None:intergenic 45.0%
GATACAGTGGTAACTACTCC+AGG + chr3.2:69956982-69957001 None:intergenic 45.0%
GATACAGTTGGAGAACCAGA+AGG - chr3.2:69957291-69957310 MS.gene25856:CDS 45.0%
TTAGTGATCCCAGTTGCAAC+AGG - chr3.2:69956689-69956708 MS.gene25856:intron 45.0%
TTGGGATGTATAACACGACC+TGG + chr3.2:69956148-69956167 None:intergenic 45.0%
! CCATTGTCTAGTTCACCTTC+TGG + chr3.2:69957309-69957328 None:intergenic 45.0%
GAGAAGGCTGCAGATACAGT+TGG - chr3.2:69957279-69957298 MS.gene25856:CDS 50.0%
GGTGACAAAAGGCTGAAACC+TGG - chr3.2:69956961-69956980 MS.gene25856:intron 50.0%
CAGGTGTGCATGCACGCATT+CGG - chr3.2:69956881-69956900 MS.gene25856:intron 55.0%
GGCTTCAAGGTTGAGAGACC+AGG - chr3.2:69956532-69956551 MS.gene25856:intron 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 69955983 69957371 69955983 ID=MS.gene25856
chr3.2 mRNA 69955983 69957371 69955983 ID=MS.gene25856.t1;Parent=MS.gene25856
chr3.2 exon 69957092 69957371 69957092 ID=MS.gene25856.t1.exon1;Parent=MS.gene25856.t1
chr3.2 CDS 69957092 69957371 69957092 ID=cds.MS.gene25856.t1;Parent=MS.gene25856.t1
chr3.2 exon 69956781 69956845 69956781 ID=MS.gene25856.t1.exon2;Parent=MS.gene25856.t1
chr3.2 CDS 69956781 69956845 69956781 ID=cds.MS.gene25856.t1;Parent=MS.gene25856.t1
chr3.2 exon 69956644 69956704 69956644 ID=MS.gene25856.t1.exon3;Parent=MS.gene25856.t1
chr3.2 CDS 69956644 69956704 69956644 ID=cds.MS.gene25856.t1;Parent=MS.gene25856.t1
chr3.2 exon 69956330 69956476 69956330 ID=MS.gene25856.t1.exon4;Parent=MS.gene25856.t1
chr3.2 CDS 69956330 69956476 69956330 ID=cds.MS.gene25856.t1;Parent=MS.gene25856.t1
chr3.2 exon 69955983 69956116 69955983 ID=MS.gene25856.t1.exon5;Parent=MS.gene25856.t1
chr3.2 CDS 69955983 69956116 69955983 ID=cds.MS.gene25856.t1;Parent=MS.gene25856.t1
Gene Sequence

>MS.gene25856

ATGGAAGGCATTGTAAAATCTCTCTACCTAACAAAAATTCCAAAGTCTCAAACATGTTCTGCTTCTCTGTTGAACTCACCAATTTTATTACACTCCAATGCCACTTCAGTAACTTTCAGTTCAGCAACAAATCATCTGCACAAGTGCTTTCTTTTTCAGCAACCAGGTCGTGTTATACATCCCAAATCACAGGAAAAAGTGAACCTAGGAGCTATACATGCATCAAAAGCTACCACTCCAACAACTAATGCTACACAAAGATGGATTCTTGAACCAGTTGGTTAGTTACACTTTCTTCTTCAATCATATCTAGAAACCAATTGAAAAATTGTTAAAATCAATATCTACAATAGCAAACTTTACCATTTTTCTTATCTTGATTGAGTAGATAAATGAGCTCAATCCTTCATGATATTACTACAAATAGGACTCCCTCAATTAACCACTTTTTAAGTATTTGTTTCAATATGGTCAATATTAACAAGGTGTTTTATCACTTTGGAAACAAAACCAATCATTTTGATAGGAGATGGTGATTCAAGGCATATAGGCTTCAAGGTTGAGAGACCAGGTGCTTATGAAATTACTTCTGTAAGTAATATCAGAATCAATGATTTGTGTGGCTGAGTATGCTTCAGACTAACTTTCTTGTTTTTGAATTATCTAGAATGAAGTCACTGTAGGACGTGTTCCAGAGAAAGCTGATTTAGTGATCCCAGTTGCAACAGGTATAGAAGTTCAAGTTAATAAACTGTTGTGTATTTCGATTCCTATTTTTCTTACAGTTACAAACATTTTTTCCACTGAAATAAGCTCCTTAATCTTAATGTACTATGCTTTGTTTATGTTCCAGAGAATCACAATATAATTTGAATAAATTGTTTTGTATTTACAGTGTCAGGTGTGCATGCACGCATTCGGATGAAACAAGACAATCTTCTTGTTATAGATTTGGATAGCACCAATGGAACATTCATTGGTGACAAAAGGCTGAAACCTGGAGTAGTTACCACTGTATCTTCTGGGAGTTATATTACTTTTGGTACTACTCTAACTCTCTTTTTATTTATTAGTTGCTTACCTTTTCTGTTGAATAATTATGTTAGTTTTTTTTCATTATACACATAATTATATAACCCTGTTTGTAGAAGGATCTTAAAAGACTCCATATTCCTAAGAGAATTTCAAAATGATAAACTAATCATCACTACAAGTACCCTATTCCATATTTAACCTCTGCTATTATGAATGCCAGGGGACACTAATTTGGCTATGTTCCGTGTGTCGAAAATAGAAGAGAAGGCTGCAGATACAGTTGGAGAACCAGAAGGTGAACTAGACAATGGTGACAAATCTGATAGTACTGAAACAAGTTCAACTTCTAGTTGA

Protein sequence

>MS.gene25856.t1

MEGIVKSLYLTKIPKSQTCSASLLNSPILLHSNATSVTFSSATNHLHKCFLFQQPGRVIHPKSQEKVNLGAIHASKATTPTTNATQRWILEPVGDGDSRHIGFKVERPGAYEITSNEVTVGRVPEKADLVIPVATVSGVHARIRMKQDNLLVIDLDSTNGTFIGDKRLKPGVVTTVSSGSYITFGDTNLAMFRVSKIEEKAADTVGEPEGELDNGDKSDSTETSSTSS