AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0189950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0189950 MtrunA17_Chr2g0279481 98.978 685 6 1 1 684 1 685 0.0 1411
Msa0189950 MtrunA17_Chr4g0050021 80.904 686 112 9 1 684 1 669 0.0 1110
Msa0189950 MtrunA17_Chr8g0382231 41.888 678 300 12 3 663 13 613 2.61e-180 527
Msa0189950 MtrunA17_Chr2g0303541 38.889 666 357 11 23 677 5 631 1.87e-151 453
Msa0189950 MtrunA17_Chr2g0285511 38.202 623 325 11 19 635 1 569 5.81e-137 415
Msa0189950 MtrunA17_Chr2g0320691 37.157 619 332 12 27 632 6 580 1.21e-131 402
Msa0189950 MtrunA17_Chr5g0401341 41.231 536 248 11 23 548 5 483 4.66e-129 395
Msa0189950 MtrunA17_Chr5g0401451 36.780 590 298 7 32 612 28 551 9.04e-126 387
Msa0189950 MtrunA17_Chr8g0374011 35.945 651 331 12 34 639 10 619 1.31e-125 387
Msa0189950 MtrunA17_Chr4g0010091 36.084 618 332 9 33 645 24 583 4.26e-125 384
Msa0189950 MtrunA17_Chr1g0207461 38.716 545 276 11 23 558 5 500 2.13e-121 375
Msa0189950 MtrunA17_Chr1g0150191 38.806 536 267 10 19 547 6 487 3.95e-121 374
Msa0189950 MtrunA17_Chr1g0181181 37.430 537 277 8 23 555 5 486 3.40e-119 367
Msa0189950 MtrunA17_Chr8g0367891 40.217 552 240 10 23 552 5 488 4.23e-119 368
Msa0189950 MtrunA17_Chr1g0191711 34.171 597 286 13 33 622 47 543 1.29e-116 360
Msa0189950 MtrunA17_Chr4g0053841 36.227 599 300 8 32 620 1 527 2.67e-115 358
Msa0189950 MtrunA17_Chr4g0076521 37.665 531 274 8 23 552 5 479 5.30e-111 347
Msa0189950 MtrunA17_Chr5g0412591 38.156 553 255 11 23 552 5 493 1.02e-109 344
Msa0189950 MtrunA17_Chr1g0195911 32.609 644 355 12 20 647 13 593 1.28e-109 344
Msa0189950 MtrunA17_Chr1g0199631 36.007 586 315 11 62 641 8 539 2.30e-109 342
Msa0189950 MtrunA17_Chr7g0245201 35.605 587 308 15 36 614 53 577 2.66e-109 344
Msa0189950 MtrunA17_Chr7g0268781 31.523 663 382 13 20 666 13 619 6.93e-108 340
Msa0189950 MtrunA17_Chr3g0108331 32.848 618 324 9 32 646 12 541 7.19e-102 322
Msa0189950 MtrunA17_Chr5g0432861 34.165 521 284 7 32 552 13 474 7.87e-102 323
Msa0189950 MtrunA17_Chr2g0290741 35.370 540 278 8 23 559 5 476 1.21e-100 320
Msa0189950 MtrunA17_Chr4g0003891 37.250 400 194 7 233 629 46 391 3.11e-80 262
Msa0189950 MtrunA17_Chr2g0332281 35.232 474 239 7 219 684 10 423 8.94e-79 257
Msa0189950 MtrunA17_Chr4g0037661 28.453 601 333 17 47 626 6 530 2.38e-55 198
Msa0189950 MtrunA17_Chr1g0163311 27.725 523 269 14 54 546 14 457 8.86e-52 187
Msa0189950 MtrunA17_Chr4g0023801 27.243 602 356 17 53 644 14 543 2.26e-50 184
Msa0189950 MtrunA17_Chr1g0206621 45.255 137 73 1 51 185 75 211 1.19e-31 123
Msa0189950 MtrunA17_Chr2g0280541 26.258 457 259 11 110 560 1 385 1.17e-30 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0189950 AT5G64330.1 68.138 725 154 15 1 654 2 720 0.0 944
Msa0189950 AT5G64330.2 68.252 652 133 14 1 584 2 647 0.0 844
Msa0189950 AT5G64330.3 68.430 586 124 11 57 584 6 588 0.0 759
Msa0189950 AT5G10250.2 39.124 639 278 12 30 643 36 588 1.65e-152 456
Msa0189950 AT5G10250.1 39.110 629 277 12 30 643 36 573 3.30e-149 447
Msa0189950 AT1G30440.1 38.148 637 348 10 23 643 5 611 2.56e-145 439
Msa0189950 AT5G03250.1 37.739 628 321 14 25 642 7 574 1.65e-131 400
Msa0189950 AT5G13600.1 37.500 616 319 12 19 629 1 555 1.78e-126 387
Msa0189950 AT5G67440.3 40.797 527 239 9 39 556 22 484 4.19e-124 381
Msa0189950 AT5G67440.1 40.797 527 239 9 39 556 22 484 4.19e-124 381
Msa0189950 AT3G44820.1 35.738 610 348 9 27 624 6 583 1.90e-121 377
Msa0189950 AT5G67385.1 39.205 528 262 7 32 557 13 483 3.74e-121 374
Msa0189950 AT1G67900.5 38.995 577 284 8 23 578 5 534 2.77e-120 373
Msa0189950 AT1G67900.1 38.995 577 284 8 23 578 5 534 2.77e-120 373
Msa0189950 AT1G67900.3 38.995 577 284 8 23 578 5 534 2.77e-120 373
Msa0189950 AT1G67900.6 38.995 577 284 8 23 578 5 534 2.77e-120 373
Msa0189950 AT1G67900.2 38.995 577 284 8 23 578 5 534 2.77e-120 373
Msa0189950 AT1G67900.4 38.995 577 284 8 23 578 5 534 2.77e-120 373
Msa0189950 AT2G14820.2 38.095 546 278 11 23 558 5 500 6.77e-116 362
Msa0189950 AT2G14820.1 38.095 546 278 11 23 558 5 500 6.77e-116 362
Msa0189950 AT4G31820.1 37.782 532 257 12 23 548 5 468 6.97e-116 359
Msa0189950 AT5G48800.1 35.410 610 327 7 37 642 34 580 1.67e-114 357
Msa0189950 AT5G67440.2 40.237 507 230 9 59 556 5 447 3.34e-114 354
Msa0189950 AT4G37590.1 36.348 597 280 13 23 605 5 515 1.34e-111 348
Msa0189950 AT1G03010.1 32.428 663 339 14 21 647 14 603 4.45e-111 349
Msa0189950 AT3G08570.1 34.740 616 324 12 40 646 30 576 8.08e-111 348
Msa0189950 AT1G03010.2 33.022 642 322 13 42 647 7 576 5.09e-110 345
Msa0189950 AT5G66560.1 33.333 687 343 15 28 646 3 642 6.88e-110 347
Msa0189950 AT1G03010.3 33.022 642 322 13 42 647 33 602 1.62e-109 345
Msa0189950 AT2G30520.1 35.163 583 303 8 32 614 18 525 3.25e-109 343
Msa0189950 AT5G67385.4 38.788 495 244 7 65 557 1 438 6.06e-108 338
Msa0189950 AT5G67385.5 38.788 495 244 7 65 557 1 438 6.06e-108 338
Msa0189950 AT5G67385.2 38.788 495 244 7 65 557 1 438 6.06e-108 338
Msa0189950 AT5G67385.3 38.788 495 244 7 65 557 1 438 6.06e-108 338
Msa0189950 AT5G47800.3 36.571 525 277 7 23 546 5 474 4.95e-105 331
Msa0189950 AT5G47800.1 36.571 525 277 7 23 546 5 474 4.95e-105 331
Msa0189950 AT5G47800.4 36.571 525 277 7 23 546 5 474 4.95e-105 331
Msa0189950 AT5G47800.2 36.571 525 277 7 23 546 118 587 1.90e-103 330
Msa0189950 AT5G66560.3 33.687 659 322 15 56 646 2 613 1.31e-102 327
Msa0189950 AT2G47860.1 32.558 645 324 16 17 624 2 572 1.34e-102 327
Msa0189950 AT3G26490.1 35.856 555 279 12 25 552 7 511 8.44e-102 323
Msa0189950 AT3G26490.3 35.856 555 279 12 25 552 7 511 8.44e-102 323
Msa0189950 AT3G26490.4 35.856 555 279 12 25 552 7 511 8.44e-102 323
Msa0189950 AT2G23050.1 36.704 534 242 10 23 548 5 450 1.11e-101 320
Msa0189950 AT3G08660.1 33.929 616 302 16 40 646 30 549 1.27e-101 323
Msa0189950 AT4G31820.2 37.474 483 229 11 71 548 1 415 1.69e-101 320
Msa0189950 AT5G66560.4 35.214 585 308 13 86 646 66 603 5.19e-99 317
Msa0189950 AT2G47860.3 31.437 668 324 17 17 624 2 595 1.95e-97 314
Msa0189950 AT2G30520.2 34.122 592 308 10 64 649 2 517 1.19e-95 306
Msa0189950 AT2G30520.3 34.122 592 308 10 64 649 2 517 1.19e-95 306
Msa0189950 AT3G50840.1 33.225 614 326 13 34 639 5 542 1.52e-94 304
Msa0189950 AT3G08660.3 34.862 545 257 13 86 621 15 470 1.79e-91 294
Msa0189950 AT5G03250.2 35.057 522 271 13 129 642 1 462 7.66e-89 286
Msa0189950 AT3G49970.1 33.214 560 260 10 56 614 6 452 4.70e-88 285
Msa0189950 AT3G49970.2 33.214 560 260 10 56 614 6 452 4.70e-88 285
Msa0189950 AT3G50840.2 32.990 582 307 12 65 639 1 506 3.38e-83 273
Msa0189950 AT3G50840.3 32.990 582 307 12 65 639 1 506 3.38e-83 273
Msa0189950 AT5G66560.2 34.170 518 240 12 34 497 9 479 3.46e-79 261
Msa0189950 AT3G15570.1 33.546 471 247 9 233 684 29 452 2.39e-78 258
Msa0189950 AT3G26490.2 33.533 501 256 12 25 498 7 457 1.35e-76 254
Msa0189950 AT2G47860.4 33.140 516 236 14 17 497 2 443 1.65e-76 252
Msa0189950 AT1G52770.1 33.532 504 258 13 206 684 3 454 2.88e-75 249
Msa0189950 AT3G08660.2 34.115 469 219 11 40 499 30 417 7.32e-75 248
Msa0189950 AT2G47860.6 31.540 539 237 15 17 497 2 466 2.33e-71 239
Msa0189950 AT2G23050.2 34.679 421 187 8 129 548 1 334 4.23e-69 230
Msa0189950 AT2G47860.5 30.153 524 268 12 129 624 1 454 1.69e-65 225
Msa0189950 AT2G47860.2 30.153 524 268 12 129 624 1 454 1.69e-65 225
Msa0189950 AT1G50280.1 28.770 504 289 10 53 542 13 460 1.60e-49 181
Msa0189950 AT1G50280.2 29.399 466 264 9 86 542 18 427 1.01e-48 179
Msa0189950 AT5G17580.2 27.451 510 291 11 46 549 22 458 2.37e-41 159
Msa0189950 AT5G17580.1 27.255 510 292 10 46 549 7 443 4.95e-41 158
Msa0189950 AT3G03510.1 26.344 558 276 16 98 648 8 437 1.31e-39 152
Msa0189950 AT3G22104.1 26.869 495 275 13 46 533 6 420 1.52e-39 153
Msa0189950 AT3G19850.1 35.115 262 152 3 341 602 273 516 3.27e-38 150
Msa0189950 AT3G19850.1 32.143 168 98 2 86 240 52 216 8.63e-17 84.7
Msa0189950 AT5G48130.1 33.333 240 143 5 37 272 32 258 5.68e-36 144
Msa0189950 AT5G48130.1 34.921 126 79 1 432 557 356 478 1.41e-18 90.5
Msa0189950 AT3G22104.4 25.532 423 231 10 115 533 3 345 3.46e-27 115
Msa0189950 AT3G22104.2 25.532 423 231 10 115 533 3 345 3.46e-27 115
Msa0189950 AT3G22104.3 25.532 423 231 10 115 533 3 345 3.46e-27 115
Msa0189950 AT3G49900.2 36.306 157 88 5 34 184 55 205 8.92e-16 81.3
Msa0189950 AT3G49900.1 36.306 157 88 5 34 184 55 205 8.99e-16 81.3

Find 131 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAACAGCAGGGAACATTTC+TGG 0.175923 2_1:+5391983 Msa0189950:CDS
CCACTCTTGCTTTAGCATTA+AGG 0.194475 2_1:-5392972 None:intergenic
GAGAATGGCAATTATGTTAA+AGG 0.217758 2_1:+5392723 Msa0189950:CDS
TAGAGTCATTACTGCTATTA+AGG 0.220033 2_1:+5392387 Msa0189950:CDS
TCTTTCATTGACAAAGTTTC+AGG 0.239657 2_1:+5393041 Msa0189950:CDS
AAAATGAAAGGCTTCCATTA+AGG 0.246972 2_1:+5393809 Msa0189950:CDS
ATGCATCAATTGAGAGTTTC+TGG 0.254350 2_1:-5393771 None:intergenic
AGCAATTCCATAGCAGAATT+TGG 0.262431 2_1:-5391954 None:intergenic
TCTTTACCTGTTCTGCAAAT+AGG 0.263165 2_1:-5393846 None:intergenic
AGTTATGAGTTTATTAGATT+TGG 0.263352 2_1:-5390955 None:intergenic
TAGCAGTTGTCCATCCTTCT+TGG 0.266447 2_1:-5393972 None:intergenic
TTAATTCCTTGTTATAATAA+AGG 0.275835 2_1:+5392820 Msa0189950:CDS
AAAATTGTTGGAGGTGTTAC+AGG 0.276384 2_1:-5391029 None:intergenic
ATTACTGCTATTAAGGTAAA+AGG 0.277571 2_1:+5392394 Msa0189950:CDS
ACTTGCACAACTACCCTTAA+TGG 0.282327 2_1:-5393823 None:intergenic
GGAATGAGATTTGAGTTAAT+TGG 0.282402 2_1:+5392415 Msa0189950:CDS
ACAAAATGTACTGTTCTAAA+AGG 0.286994 2_1:-5391185 None:intergenic
CTAATCCAAAAGGGATAAAA+TGG 0.293832 2_1:+5392218 Msa0189950:CDS
TTTGGAGCTTCAAAATGATA+TGG 0.295839 2_1:+5394047 Msa0189950:CDS
TAATGCATTATGCAACTAAA+TGG 0.300901 2_1:+5392446 Msa0189950:CDS
TTTCATGCACTTTCCTTCTT+AGG 0.307187 2_1:+5392695 Msa0189950:CDS
CTGTTCTGTTCTGGAGCTGC+TGG 0.323542 2_1:-5394219 None:intergenic
GGTAGCGGTAGTTGGATTAA+AGG 0.337161 2_1:+5392547 Msa0189950:CDS
GAGTGTGAAAGCTAAGTATT+TGG 0.342226 2_1:+5394029 Msa0189950:CDS
GTAGTAGTGGTAGCGGTAGT+TGG 0.346625 2_1:+5392539 Msa0189950:CDS
AGCTATTGATTCCTATCTTA+AGG 0.367444 2_1:+5393119 Msa0189950:CDS
CCTTCTTAGGCTTCTGAGAA+TGG 0.374018 2_1:+5392708 Msa0189950:CDS
AAATGAAAGGCTTCCATTAA+GGG 0.389974 2_1:+5393810 Msa0189950:CDS
TCACAGGTTCTAGCTGATTC+AGG 0.390652 2_1:-5393081 None:intergenic
TTCTGAGAAACATATGGGAT+TGG 0.397986 2_1:+5392942 Msa0189950:CDS
GCAAGTCCTATTTGCAGAAC+AGG 0.399215 2_1:+5393840 Msa0189950:CDS
TTGAAGATGTTTCAATCCTT+AGG 0.401499 2_1:+5392350 Msa0189950:CDS
TCTGAGAAACATATGGGATT+GGG 0.407788 2_1:+5392943 Msa0189950:CDS
CATATATGAATCACGCGAAC+CGG 0.412776 2_1:+5391871 Msa0189950:CDS
TGAGTTTATTAGATTTGGAT+TGG 0.415940 2_1:-5390950 None:intergenic
GGAAGTAATTACCTTAAGAT+AGG 0.421004 2_1:-5393130 None:intergenic
ATATCATTATTATTCCATCT+TGG 0.423799 2_1:-5392277 None:intergenic
GATTCAACATCTTTCAAAGA+AGG 0.425349 2_1:-5393889 None:intergenic
TGTTACAGGCTGTGATGAAA+TGG 0.431885 2_1:-5391015 None:intergenic
CTTTCATGCTCAGAAAGTGT+TGG 0.432520 2_1:-5393721 None:intergenic
TAAATAAGTTGAGTGAGAAT+AGG 0.435374 2_1:-5391129 None:intergenic
TTTCCACTTCGAGATATAAG+AGG 0.442402 2_1:-5391836 None:intergenic
TTCTTCAAAGAGGTCACTCT+TGG 0.443338 2_1:+5391324 Msa0189950:CDS
AACATCTTTCAAAGAAGGGT+TGG 0.446054 2_1:-5393884 None:intergenic
GGTTACTGAATTAGAGAAAA+GGG 0.454667 2_1:+5392759 Msa0189950:CDS
ATAACTATCTTATTCACATC+CGG 0.457064 2_1:-5391890 None:intergenic
AGGCATAAAGACACTGAAAC+TGG 0.458972 2_1:+5390882 Msa0189950:five_prime_UTR
AAAGCTTGTGCTAATCCAAA+AGG 0.459789 2_1:+5392208 Msa0189950:CDS
ATTGCTGTTGATTTAACAGC+AGG 0.465524 2_1:+5391971 Msa0189950:CDS
ATCAACTAGTTCCTCCTGAT+TGG 0.465695 2_1:+5392323 Msa0189950:CDS
GAAGCGACTCTGCCGCGTAA+TGG 0.466966 2_1:+5393744 Msa0189950:CDS
TCAACTGCAAGACACAAGTA+TGG 0.473817 2_1:+5391254 Msa0189950:CDS
ATTCAACATCTTTCAAAGAA+GGG 0.475157 2_1:-5393888 None:intergenic
AACTAGTTCCTCCTGATTGG+TGG 0.478674 2_1:+5392326 Msa0189950:CDS
AGCTAACTTCCACTTGCATA+AGG 0.482491 2_1:+5391520 Msa0189950:CDS
ACATCTTCAAACCACCAATC+AGG 0.482701 2_1:-5392337 None:intergenic
GAAGAAACACATGGAATGGT+TGG 0.485434 2_1:-5391107 None:intergenic
TTACAGAGGTTTCAAGAGAC+AGG 0.493241 2_1:+5393016 Msa0189950:CDS
ATGACAGAAGATTTAGAAGA+AGG 0.494776 2_1:+5392034 Msa0189950:CDS
TGGTTACTGAATTAGAGAAA+AGG 0.496556 2_1:+5392758 Msa0189950:CDS
TGATGATGTTCTTCCTGTGT+AGG 0.497579 2_1:-5392242 None:intergenic
TGTAACCATTGGTTGAGTAA+AGG 0.498751 2_1:-5393911 None:intergenic
TCAGCTAGAACCTGTGATGA+TGG 0.501666 2_1:+5393087 Msa0189950:CDS
TATGTGTAACTGTATTAGTA+AGG 0.501677 2_1:-5390911 None:intergenic
GGAACTAGTTGATTCCTACT+TGG 0.503593 2_1:-5392313 None:intergenic
CATACTTCAGCTTGGAGTAG+TGG 0.506325 2_1:+5394111 Msa0189950:CDS
TCTAATTCAGTAACCAATGC+AGG 0.508323 2_1:-5392751 None:intergenic
GGATGTGAATAAGATAGTTA+TGG 0.510860 2_1:+5391892 Msa0189950:CDS
GTTAAAGGTTGCACCTGCAT+TGG 0.512074 2_1:+5392738 Msa0189950:CDS
AACTAAATGGCTACAAGGGT+TGG 0.513326 2_1:+5392459 Msa0189950:CDS
AATAGTAATAGTAGTAGTAG+TGG 0.515974 2_1:+5392526 Msa0189950:CDS
AGCAGAAGCATACTTCAGCT+TGG 0.516992 2_1:+5394103 Msa0189950:CDS
AATAGTAGTAGTAGTGGTAG+CGG 0.517826 2_1:+5392532 Msa0189950:CDS
AGTTGGATTAAAGGCGGCGG+TGG 0.518651 2_1:+5392556 Msa0189950:CDS
GTCTCTCCTTTATTATAACA+AGG 0.523236 2_1:-5392826 None:intergenic
CTTCAGCTTGGAGTAGTGGT+TGG 0.523301 2_1:+5394115 Msa0189950:CDS
TTCGATGAAGCGAGCAATTG+TGG 0.530246 2_1:+5392502 Msa0189950:CDS
TGATATCCCTGGTGGATTTG+AGG 0.532058 2_1:+5391916 Msa0189950:CDS
TAACAAAGTGATCAATCCTA+AGG 0.539725 2_1:-5392366 None:intergenic
CTTGCAGCCAAATTCTGCTA+TGG 0.542630 2_1:+5391947 Msa0189950:CDS
CAACACTTTCTGAGCATGAA+AGG 0.546369 2_1:+5393722 Msa0189950:CDS
CCATTCTCAGAAGCCTAAGA+AGG 0.546533 2_1:-5392708 None:intergenic
TATGCAACTAAATGGCTACA+AGG 0.547334 2_1:+5392454 Msa0189950:CDS
GGACTTCACATGATTGTAGC+AGG 0.547821 2_1:+5392577 Msa0189950:CDS
ACTGAATTAGAGAAAAGGGT+TGG 0.548384 2_1:+5392763 Msa0189950:CDS
TCAGATTCCCACATGTTGGA+TGG 0.549036 2_1:-5391224 None:intergenic
TCTATAAAGTCCATCATCAC+AGG 0.549509 2_1:-5393097 None:intergenic
CAACACACTAAAGTTTGAGC+TGG 0.551730 2_1:+5394005 Msa0189950:CDS
CATCCTTCTTGGAATGATTG+TGG 0.555236 2_1:-5393961 None:intergenic
AGATAGCTACCTTATGCAAG+TGG 0.556587 2_1:-5391529 None:intergenic
ATTTGAAGATTCTCTGACCA+AGG 0.556867 2_1:-5392154 None:intergenic
ACTGAAGGATTTCTTCAAAG+AGG 0.557934 2_1:+5391314 Msa0189950:CDS
CCTTAATGCTAAAGCAAGAG+TGG 0.561105 2_1:+5392972 Msa0189950:CDS
CACAATCATTCCAAGAAGGA+TGG 0.563573 2_1:+5393962 Msa0189950:CDS
ATAGGAAGAAACACATGGAA+TGG 0.563622 2_1:-5391111 None:intergenic
GCTGCTGGAATATTACTTTG+AGG 0.565988 2_1:-5394204 None:intergenic
AAGCTTGTGCTAATCCAAAA+GGG 0.570071 2_1:+5392209 Msa0189950:CDS
ATTTGAACAAGAAAATCACT+TGG 0.572128 2_1:-5391491 None:intergenic
ACTCTCAGATTCCCACATGT+TGG 0.572140 2_1:-5391228 None:intergenic
TGAATCCTTTACTCAACCAA+TGG 0.578454 2_1:+5393906 Msa0189950:CDS
GTTACAGGCTGTGATGAAAT+GGG 0.583692 2_1:-5391014 None:intergenic
AAACATGGTGTCAAGACTGA+AGG 0.585042 2_1:+5391299 Msa0189950:CDS
GCAAGACACAAGTATGGTGA+TGG 0.587211 2_1:+5391260 Msa0189950:CDS
ATAGTTATGGATGATATCCC+TGG 0.588860 2_1:+5391905 Msa0189950:CDS
GATGCAGCGAGTCTATAGCT+TGG 0.589137 2_1:+5392185 Msa0189950:CDS
TCAAATGCCTCAAATCCACC+AGG 0.589531 2_1:-5391923 None:intergenic
TATCCTCTTATATCTCGAAG+TGG 0.591595 2_1:+5391833 Msa0189950:CDS
TGAAAATGAAAAGACATGTA+TGG 0.594971 2_1:+5390857 Msa0189950:five_prime_UTR
ATGCTAAAGCAAGAGTGGCA+AGG 0.595719 2_1:+5392977 Msa0189950:CDS
CATGAAACAGTATCTTTCTG+AGG 0.597213 2_1:-5392679 None:intergenic
TGCATGCTGCACAAAATGAA+AGG 0.608887 2_1:+5393797 Msa0189950:CDS
GGGATAAAATGGTCCTACAC+AGG 0.610206 2_1:+5392229 Msa0189950:CDS
TGAAAGATACAAGTCCAAGT+AGG 0.614508 2_1:+5392299 Msa0189950:CDS
TGTTGACAGTTATCTTACAG+AGG 0.625756 2_1:+5393002 Msa0189950:CDS
ATGAAAAGACATGTATGGTG+AGG 0.631369 2_1:+5390862 Msa0189950:five_prime_UTR
GAAACAGTATCTTTCTGAGG+AGG 0.634508 2_1:-5392676 None:intergenic
ATGCAACTAAATGGCTACAA+GGG 0.636403 2_1:+5392455 Msa0189950:CDS
ACACCACAATCATTCCAAGA+AGG 0.638741 2_1:+5393958 Msa0189950:CDS
TTGCTGTTGATTTAACAGCA+GGG 0.649818 2_1:+5391972 Msa0189950:CDS
GTCTTCAAGCTAAAGAACAA+AGG 0.650300 2_1:+5392623 Msa0189950:CDS
CTTCACATGATTGTAGCAGG+TGG 0.651151 2_1:+5392580 Msa0189950:CDS
GCTGTGAGAAGCTTTCACCT+TGG 0.658908 2_1:+5392137 Msa0189950:CDS
TCTTCAAACCACCAATCAGG+AGG 0.664130 2_1:-5392334 None:intergenic
ATTCTCACTTCAAACAAACA+TGG 0.669333 2_1:+5391284 Msa0189950:CDS
TGAGAATAGGAAGAAACACA+TGG 0.672730 2_1:-5391116 None:intergenic
GTTATGGATGATATCCCTGG+TGG 0.677226 2_1:+5391908 Msa0189950:CDS
ATGATGTTGATCTTGTTATG+CGG 0.690885 2_1:+5392857 Msa0189950:CDS
GTTTCTGGCAATCCATTACG+CGG 0.691677 2_1:-5393756 None:intergenic
GGTAGTTGGATTAAAGGCGG+CGG 0.693718 2_1:+5392553 Msa0189950:CDS
AGCGGTAGTTGGATTAAAGG+CGG 0.709972 2_1:+5392550 Msa0189950:CDS
CAAATGCCTCAAATCCACCA+GGG 0.743591 2_1:-5391922 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTATCTTAAAAAATTGT+CGG - chr2_1:5391078-5391097 None:intergenic 15.0%
!!! AGAATCTAGTATTTTTTTAA+TGG + chr2_1:5391404-5391423 Msa0189950:intron 15.0%
!!! TTAATTCCTTGTTATAATAA+AGG + chr2_1:5392820-5392839 Msa0189950:CDS 15.0%
!!! ATGTTTTATCTTATTTTAAG+TGG + chr2_1:5393231-5393250 Msa0189950:intron 15.0%
!! TTCAAAATAATTCTTGAAAT+TGG + chr2_1:5393469-5393488 Msa0189950:intron 15.0%
!! AGTTATGAGTTTATTAGATT+TGG - chr2_1:5390958-5390977 None:intergenic 20.0%
!! AACAAAGAACAAAAAATTGT+TGG - chr2_1:5391044-5391063 None:intergenic 20.0%
!!! TCACTCAACTTATTTATTTT+TGG + chr2_1:5391136-5391155 Msa0189950:five_prime_UTR 20.0%
!!! ATTCTTAAAAGCATTTTCAA+AGG - chr2_1:5391389-5391408 None:intergenic 20.0%
!! ACCTATGAGTTTATAATTTA+TGG + chr2_1:5391592-5391611 Msa0189950:intron 20.0%
!! TCCATAAATTATAAACTCAT+AGG - chr2_1:5391596-5391615 None:intergenic 20.0%
!!! AAGTTGAACAAAATTTTGTA+CGG + chr2_1:5391701-5391720 Msa0189950:intron 20.0%
!!! TTTTTATACTAAGAGAGATT+AGG + chr2_1:5391747-5391766 Msa0189950:intron 20.0%
!! ATATCATTATTATTCCATCT+TGG - chr2_1:5392280-5392299 None:intergenic 20.0%
!!! TTACTTATTTACCTTTTCAA+TGG + chr2_1:5393165-5393184 Msa0189950:intron 20.0%
!!! TCAATTCAAAGACTATTTTA+GGG + chr2_1:5393576-5393595 Msa0189950:intron 20.0%
!!! TTGAGATTTTCTTATCTTAT+AGG - chr2_1:5394284-5394303 None:intergenic 20.0%
! TGAAAATGAAAAGACATGTA+TGG + chr2_1:5390857-5390876 Msa0189950:five_prime_UTR 25.0%
! TATGTGTAACTGTATTAGTA+AGG - chr2_1:5390914-5390933 None:intergenic 25.0%
! TGAGTTTATTAGATTTGGAT+TGG - chr2_1:5390953-5390972 None:intergenic 25.0%
! AAAGAACAAAAAATTGTTGG+AGG - chr2_1:5391041-5391060 None:intergenic 25.0%
!! TAAATAAGTTGAGTGAGAAT+AGG - chr2_1:5391132-5391151 None:intergenic 25.0%
!! ACAAAATGTACTGTTCTAAA+AGG - chr2_1:5391188-5391207 None:intergenic 25.0%
! ATCTAGTATTATTCTAATGC+AGG + chr2_1:5391450-5391469 Msa0189950:intron 25.0%
! ATTTGAACAAGAAAATCACT+TGG - chr2_1:5391494-5391513 None:intergenic 25.0%
!! AGTGATTTTCTTGTTCAAAT+TGG + chr2_1:5391494-5391513 Msa0189950:CDS 25.0%
! AACACAAATAAATTCATCAG+TGG - chr2_1:5391789-5391808 None:intergenic 25.0%
! ATAACTATCTTATTCACATC+CGG - chr2_1:5391893-5391912 None:intergenic 25.0%
! ATTACTGCTATTAAGGTAAA+AGG + chr2_1:5392394-5392413 Msa0189950:CDS 25.0%
! TAATGCATTATGCAACTAAA+TGG + chr2_1:5392446-5392465 Msa0189950:CDS 25.0%
! AATAGTAATAGTAGTAGTAG+TGG + chr2_1:5392526-5392545 Msa0189950:CDS 25.0%
!!! TCATTTTCTGAGAAACATAT+GGG + chr2_1:5392937-5392956 Msa0189950:CDS 25.0%
! AATAAGTAAATCCTAGAAAC+AGG - chr2_1:5393154-5393173 None:intergenic 25.0%
! ATGACATAAAACCATTGAAA+AGG - chr2_1:5393179-5393198 None:intergenic 25.0%
! AGTGGATAAATATACATAGA+AGG + chr2_1:5393249-5393268 Msa0189950:intron 25.0%
!! CTCAATTCAAAGACTATTTT+AGG + chr2_1:5393575-5393594 Msa0189950:intron 25.0%
!!! GTATATTGATGATGAATGTA+TGG + chr2_1:5393636-5393655 Msa0189950:intron 25.0%
! ATTCAACATCTTTCAAAGAA+GGG - chr2_1:5393891-5393910 None:intergenic 25.0%
ATTCTCACTTCAAACAAACA+TGG + chr2_1:5391284-5391303 Msa0189950:CDS 30.0%
!! GCCTTTACTTTGCTTTTATA+CGG + chr2_1:5391632-5391651 Msa0189950:intron 30.0%
TCCGTATAAAAGCAAAGTAA+AGG - chr2_1:5391636-5391655 None:intergenic 30.0%
!! GGACTTAAATTCGTTTTTGT+AGG + chr2_1:5391653-5391672 Msa0189950:intron 30.0%
GGATGTGAATAAGATAGTTA+TGG + chr2_1:5391892-5391911 Msa0189950:CDS 30.0%
ATGACAGAAGATTTAGAAGA+AGG + chr2_1:5392034-5392053 Msa0189950:CDS 30.0%
!!! GCTATGTTGTTTTATCATCA+TGG + chr2_1:5392089-5392108 Msa0189950:CDS 30.0%
CTAATCCAAAAGGGATAAAA+TGG + chr2_1:5392218-5392237 Msa0189950:CDS 30.0%
! CAAAAATTTCAAGTCCAAGA+TGG + chr2_1:5392263-5392282 Msa0189950:CDS 30.0%
TTGAAGATGTTTCAATCCTT+AGG + chr2_1:5392350-5392369 Msa0189950:CDS 30.0%
TAACAAAGTGATCAATCCTA+AGG - chr2_1:5392369-5392388 None:intergenic 30.0%
TAGAGTCATTACTGCTATTA+AGG + chr2_1:5392387-5392406 Msa0189950:CDS 30.0%
! GGAATGAGATTTGAGTTAAT+TGG + chr2_1:5392415-5392434 Msa0189950:CDS 30.0%
GAGAATGGCAATTATGTTAA+AGG + chr2_1:5392723-5392742 Msa0189950:CDS 30.0%
TGGTTACTGAATTAGAGAAA+AGG + chr2_1:5392758-5392777 Msa0189950:CDS 30.0%
GGTTACTGAATTAGAGAAAA+GGG + chr2_1:5392759-5392778 Msa0189950:CDS 30.0%
GTCTCTCCTTTATTATAACA+AGG - chr2_1:5392829-5392848 None:intergenic 30.0%
! ATGATGTTGATCTTGTTATG+CGG + chr2_1:5392857-5392876 Msa0189950:CDS 30.0%
! TCAGAAAATGACTGTCTTTT+CGG - chr2_1:5392928-5392947 None:intergenic 30.0%
!! GTCATTTTCTGAGAAACATA+TGG + chr2_1:5392936-5392955 Msa0189950:CDS 30.0%
TCTTTCATTGACAAAGTTTC+AGG + chr2_1:5393041-5393060 Msa0189950:CDS 30.0%
!! AGCTATTGATTCCTATCTTA+AGG + chr2_1:5393119-5393138 Msa0189950:CDS 30.0%
GGAAGTAATTACCTTAAGAT+AGG - chr2_1:5393133-5393152 None:intergenic 30.0%
!! TTTGCAAAAGTACTGCAAAT+TGG - chr2_1:5393329-5393348 None:intergenic 30.0%
! TTTGCAGTACTTTTGCAAAT+TGG + chr2_1:5393330-5393349 Msa0189950:intron 30.0%
GCACTTAATATCCATTAGTA+TGG + chr2_1:5393356-5393375 Msa0189950:intron 30.0%
TCTACTCATTTCCATACTAA+TGG - chr2_1:5393370-5393389 None:intergenic 30.0%
CTACAATTTCAAAGGCAAAT+CGG - chr2_1:5393414-5393433 None:intergenic 30.0%
TCAGAACTCTACAATTTCAA+AGG - chr2_1:5393422-5393441 None:intergenic 30.0%
! ACGAAATTGACTGACTTAAA+AGG + chr2_1:5393533-5393552 Msa0189950:intron 30.0%
TGGTGATTCACTCAAATATT+AGG + chr2_1:5393606-5393625 Msa0189950:intron 30.0%
TTCACTAGTAAGAAATCCAA+TGG - chr2_1:5393683-5393702 None:intergenic 30.0%
TACTAGTGAAGTAAACTAAC+AGG + chr2_1:5393693-5393712 Msa0189950:intron 30.0%
AAAATGAAAGGCTTCCATTA+AGG + chr2_1:5393809-5393828 Msa0189950:CDS 30.0%
AAATGAAAGGCTTCCATTAA+GGG + chr2_1:5393810-5393829 Msa0189950:CDS 30.0%
GATTCAACATCTTTCAAAGA+AGG - chr2_1:5393892-5393911 None:intergenic 30.0%
! TTTGGAGCTTCAAAATGATA+TGG + chr2_1:5394047-5394066 Msa0189950:CDS 30.0%
ACAGAAAAACTACTAGAAGA+TGG + chr2_1:5394238-5394257 Msa0189950:CDS 30.0%
CTTGAAAAAAGTGATTGACT+TGG - chr2_1:5394335-5394354 None:intergenic 30.0%
ATGAAAAGACATGTATGGTG+AGG + chr2_1:5390862-5390881 Msa0189950:five_prime_UTR 35.0%
!! AAAATTGTTGGAGGTGTTAC+AGG - chr2_1:5391032-5391051 None:intergenic 35.0%
ATAGGAAGAAACACATGGAA+TGG - chr2_1:5391114-5391133 None:intergenic 35.0%
TGAGAATAGGAAGAAACACA+TGG - chr2_1:5391119-5391138 None:intergenic 35.0%
!!! AGTTTTTTCCATCCAACATG+TGG + chr2_1:5391216-5391235 Msa0189950:exon 35.0%
!!! GTTTTTTCCATCCAACATGT+GGG + chr2_1:5391217-5391236 Msa0189950:exon 35.0%
ACTGAAGGATTTCTTCAAAG+AGG + chr2_1:5391314-5391333 Msa0189950:CDS 35.0%
GAGGATACTTTCACAACAAA+GGG - chr2_1:5391820-5391839 None:intergenic 35.0%
AGAGGATACTTTCACAACAA+AGG - chr2_1:5391821-5391840 None:intergenic 35.0%
TATCCTCTTATATCTCGAAG+TGG + chr2_1:5391833-5391852 Msa0189950:CDS 35.0%
TTTCCACTTCGAGATATAAG+AGG - chr2_1:5391839-5391858 None:intergenic 35.0%
ATAGTTATGGATGATATCCC+TGG + chr2_1:5391905-5391924 Msa0189950:CDS 35.0%
AGCAATTCCATAGCAGAATT+TGG - chr2_1:5391957-5391976 None:intergenic 35.0%
!! ATTGCTGTTGATTTAACAGC+AGG + chr2_1:5391971-5391990 Msa0189950:CDS 35.0%
!! TTGCTGTTGATTTAACAGCA+GGG + chr2_1:5391972-5391991 Msa0189950:CDS 35.0%
ATTTGAAGATTCTCTGACCA+AGG - chr2_1:5392157-5392176 None:intergenic 35.0%
AAAGCTTGTGCTAATCCAAA+AGG + chr2_1:5392208-5392227 Msa0189950:CDS 35.0%
AAGCTTGTGCTAATCCAAAA+GGG + chr2_1:5392209-5392228 Msa0189950:CDS 35.0%
! TAGGACCATTTTATCCCTTT+TGG - chr2_1:5392226-5392245 None:intergenic 35.0%
TGAAAGATACAAGTCCAAGT+AGG + chr2_1:5392299-5392318 Msa0189950:CDS 35.0%
TATGCAACTAAATGGCTACA+AGG + chr2_1:5392454-5392473 Msa0189950:CDS 35.0%
ATGCAACTAAATGGCTACAA+GGG + chr2_1:5392455-5392474 Msa0189950:CDS 35.0%
AATAGTAGTAGTAGTGGTAG+CGG + chr2_1:5392532-5392551 Msa0189950:CDS 35.0%
GTCTTCAAGCTAAAGAACAA+AGG + chr2_1:5392623-5392642 Msa0189950:CDS 35.0%
CATGAAACAGTATCTTTCTG+AGG - chr2_1:5392682-5392701 None:intergenic 35.0%
TTTCATGCACTTTCCTTCTT+AGG + chr2_1:5392695-5392714 Msa0189950:CDS 35.0%
TCTAATTCAGTAACCAATGC+AGG - chr2_1:5392754-5392773 None:intergenic 35.0%
ACTGAATTAGAGAAAAGGGT+TGG + chr2_1:5392763-5392782 Msa0189950:CDS 35.0%
! TTCTGAGAAACATATGGGAT+TGG + chr2_1:5392942-5392961 Msa0189950:CDS 35.0%
TCTGAGAAACATATGGGATT+GGG + chr2_1:5392943-5392962 Msa0189950:CDS 35.0%
TGTTGACAGTTATCTTACAG+AGG + chr2_1:5393002-5393021 Msa0189950:CDS 35.0%
TCTATAAAGTCCATCATCAC+AGG - chr2_1:5393100-5393119 None:intergenic 35.0%
! GGTAATTACTTCCTGTTTCT+AGG + chr2_1:5393140-5393159 Msa0189950:intron 35.0%
! GTCATCTAGAGTTACACATT+TGG + chr2_1:5393194-5393213 Msa0189950:intron 35.0%
CTAGAGTTACACATTTGGTT+TGG + chr2_1:5393199-5393218 Msa0189950:intron 35.0%
CTGACTTAAAAGGAATAACG+TGG + chr2_1:5393543-5393562 Msa0189950:intron 35.0%
!! GACTATTTTAGGGACAACTT+TGG + chr2_1:5393586-5393605 Msa0189950:intron 35.0%
CAGCTTAACTGTTATTCCAT+TGG + chr2_1:5393664-5393683 Msa0189950:intron 35.0%
ATGCATCAATTGAGAGTTTC+TGG - chr2_1:5393774-5393793 None:intergenic 35.0%
TCTTTACCTGTTCTGCAAAT+AGG - chr2_1:5393849-5393868 None:intergenic 35.0%
AACATCTTTCAAAGAAGGGT+TGG - chr2_1:5393887-5393906 None:intergenic 35.0%
TGAATCCTTTACTCAACCAA+TGG + chr2_1:5393906-5393925 Msa0189950:CDS 35.0%
! TGTAACCATTGGTTGAGTAA+AGG - chr2_1:5393914-5393933 None:intergenic 35.0%
!! GTTTTTCGGTTTGTAACCAT+TGG - chr2_1:5393925-5393944 None:intergenic 35.0%
GAGTGTGAAAGCTAAGTATT+TGG + chr2_1:5394029-5394048 Msa0189950:CDS 35.0%
! GTAGTTTTTCTGTTCTGTTC+TGG - chr2_1:5394231-5394250 None:intergenic 35.0%
AGGCATAAAGACACTGAAAC+TGG + chr2_1:5390882-5390901 Msa0189950:five_prime_UTR 40.0%
GTTACAGGCTGTGATGAAAT+GGG - chr2_1:5391017-5391036 None:intergenic 40.0%
TGTTACAGGCTGTGATGAAA+TGG - chr2_1:5391018-5391037 None:intergenic 40.0%
GAAGAAACACATGGAATGGT+TGG - chr2_1:5391110-5391129 None:intergenic 40.0%
TCAACTGCAAGACACAAGTA+TGG + chr2_1:5391254-5391273 Msa0189950:CDS 40.0%
! AAACATGGTGTCAAGACTGA+AGG + chr2_1:5391299-5391318 Msa0189950:CDS 40.0%
TTCTTCAAAGAGGTCACTCT+TGG + chr2_1:5391324-5391343 Msa0189950:CDS 40.0%
AGCTAACTTCCACTTGCATA+AGG + chr2_1:5391520-5391539 Msa0189950:CDS 40.0%
AGATAGCTACCTTATGCAAG+TGG - chr2_1:5391532-5391551 None:intergenic 40.0%
CATATATGAATCACGCGAAC+CGG + chr2_1:5391871-5391890 Msa0189950:CDS 40.0%
TTAACAGCAGGGAACATTTC+TGG + chr2_1:5391983-5392002 Msa0189950:CDS 40.0%
! TGATGATGTTCTTCCTGTGT+AGG - chr2_1:5392245-5392264 None:intergenic 40.0%
! GGAACTAGTTGATTCCTACT+TGG - chr2_1:5392316-5392335 None:intergenic 40.0%
ATCAACTAGTTCCTCCTGAT+TGG + chr2_1:5392323-5392342 Msa0189950:CDS 40.0%
ACATCTTCAAACCACCAATC+AGG - chr2_1:5392340-5392359 None:intergenic 40.0%
AACTAAATGGCTACAAGGGT+TGG + chr2_1:5392459-5392478 Msa0189950:CDS 40.0%
! GAAACAGTATCTTTCTGAGG+AGG - chr2_1:5392679-5392698 None:intergenic 40.0%
CCACTCTTGCTTTAGCATTA+AGG - chr2_1:5392975-5392994 None:intergenic 40.0%
CCTTAATGCTAAAGCAAGAG+TGG + chr2_1:5392972-5392991 Msa0189950:CDS 40.0%
TTACAGAGGTTTCAAGAGAC+AGG + chr2_1:5393016-5393035 Msa0189950:CDS 40.0%
!! CACACTCGCATAATTTGCTA+CGG + chr2_1:5393278-5393297 Msa0189950:intron 40.0%
TTCGCAAACCAATTCACGTT+TGG - chr2_1:5393304-5393323 None:intergenic 40.0%
TAAAAGGAATAACGTGGTCG+AGG + chr2_1:5393549-5393568 Msa0189950:intron 40.0%
! CTTTCATGCTCAGAAAGTGT+TGG - chr2_1:5393724-5393743 None:intergenic 40.0%
CAACACTTTCTGAGCATGAA+AGG + chr2_1:5393722-5393741 Msa0189950:CDS 40.0%
TGCATGCTGCACAAAATGAA+AGG + chr2_1:5393797-5393816 Msa0189950:CDS 40.0%
ACTTGCACAACTACCCTTAA+TGG - chr2_1:5393826-5393845 None:intergenic 40.0%
AAACCGAAAAACACTTCTCG+AGG + chr2_1:5393933-5393952 Msa0189950:CDS 40.0%
ACACCACAATCATTCCAAGA+AGG + chr2_1:5393958-5393977 Msa0189950:CDS 40.0%
CATCCTTCTTGGAATGATTG+TGG - chr2_1:5393964-5393983 None:intergenic 40.0%
CACAATCATTCCAAGAAGGA+TGG + chr2_1:5393962-5393981 Msa0189950:CDS 40.0%
CAACACACTAAAGTTTGAGC+TGG + chr2_1:5394005-5394024 Msa0189950:CDS 40.0%
GCTGCTGGAATATTACTTTG+AGG - chr2_1:5394207-5394226 None:intergenic 40.0%
TCAGATTCCCACATGTTGGA+TGG - chr2_1:5391227-5391246 None:intergenic 45.0%
ACTCTCAGATTCCCACATGT+TGG - chr2_1:5391231-5391250 None:intergenic 45.0%
GCAAGACACAAGTATGGTGA+TGG + chr2_1:5391260-5391279 Msa0189950:CDS 45.0%
GTTATGGATGATATCCCTGG+TGG + chr2_1:5391908-5391927 Msa0189950:CDS 45.0%
!! TGATATCCCTGGTGGATTTG+AGG + chr2_1:5391916-5391935 Msa0189950:CDS 45.0%
CAAATGCCTCAAATCCACCA+GGG - chr2_1:5391925-5391944 None:intergenic 45.0%
TCAAATGCCTCAAATCCACC+AGG - chr2_1:5391926-5391945 None:intergenic 45.0%
CTTGCAGCCAAATTCTGCTA+TGG + chr2_1:5391947-5391966 Msa0189950:CDS 45.0%
GGGATAAAATGGTCCTACAC+AGG + chr2_1:5392229-5392248 Msa0189950:CDS 45.0%
AACTAGTTCCTCCTGATTGG+TGG + chr2_1:5392326-5392345 Msa0189950:CDS 45.0%
TCTTCAAACCACCAATCAGG+AGG - chr2_1:5392337-5392356 None:intergenic 45.0%
TTCGATGAAGCGAGCAATTG+TGG + chr2_1:5392502-5392521 Msa0189950:CDS 45.0%
! GGTAGCGGTAGTTGGATTAA+AGG + chr2_1:5392547-5392566 Msa0189950:CDS 45.0%
AGCGGTAGTTGGATTAAAGG+CGG + chr2_1:5392550-5392569 Msa0189950:CDS 45.0%
GGACTTCACATGATTGTAGC+AGG + chr2_1:5392577-5392596 Msa0189950:CDS 45.0%
CTTCACATGATTGTAGCAGG+TGG + chr2_1:5392580-5392599 Msa0189950:CDS 45.0%
CCATTCTCAGAAGCCTAAGA+AGG - chr2_1:5392711-5392730 None:intergenic 45.0%
! CCTTCTTAGGCTTCTGAGAA+TGG + chr2_1:5392708-5392727 Msa0189950:CDS 45.0%
GTTAAAGGTTGCACCTGCAT+TGG + chr2_1:5392738-5392757 Msa0189950:CDS 45.0%
ATGCTAAAGCAAGAGTGGCA+AGG + chr2_1:5392977-5392996 Msa0189950:CDS 45.0%
! TCACAGGTTCTAGCTGATTC+AGG - chr2_1:5393084-5393103 None:intergenic 45.0%
TCAGCTAGAACCTGTGATGA+TGG + chr2_1:5393087-5393106 Msa0189950:CDS 45.0%
!! GTTTCTGGCAATCCATTACG+CGG - chr2_1:5393759-5393778 None:intergenic 45.0%
GCAAGTCCTATTTGCAGAAC+AGG + chr2_1:5393840-5393859 Msa0189950:CDS 45.0%
!!! TCGCCTCGAGAAGTGTTTTT+CGG - chr2_1:5393939-5393958 None:intergenic 45.0%
TAGCAGTTGTCCATCCTTCT+TGG - chr2_1:5393975-5393994 None:intergenic 45.0%
! AGCAGAAGCATACTTCAGCT+TGG + chr2_1:5394103-5394122 Msa0189950:CDS 45.0%
CATACTTCAGCTTGGAGTAG+TGG + chr2_1:5394111-5394130 Msa0189950:CDS 45.0%
GCTGTGAGAAGCTTTCACCT+TGG + chr2_1:5392137-5392156 Msa0189950:CDS 50.0%
GATGCAGCGAGTCTATAGCT+TGG + chr2_1:5392185-5392204 Msa0189950:CDS 50.0%
!! GTAGTAGTGGTAGCGGTAGT+TGG + chr2_1:5392539-5392558 Msa0189950:CDS 50.0%
GGTAGTTGGATTAAAGGCGG+CGG + chr2_1:5392553-5392572 Msa0189950:CDS 50.0%
TGCTACGGCCAAACGTGAAT+TGG + chr2_1:5393293-5393312 Msa0189950:intron 50.0%
CTTCAGCTTGGAGTAGTGGT+TGG + chr2_1:5394115-5394134 Msa0189950:CDS 50.0%
AGTTGGATTAAAGGCGGCGG+TGG + chr2_1:5392556-5392575 Msa0189950:CDS 55.0%
!! CTGTTCTGTTCTGGAGCTGC+TGG - chr2_1:5394222-5394241 None:intergenic 55.0%
GAAGCGACTCTGCCGCGTAA+TGG + chr2_1:5393744-5393763 Msa0189950:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 5390845 5394501 5390845 ID=Msa0189950;Name=Msa0189950
chr2_1 mRNA 5390845 5394501 5390845 ID=Msa0189950-mRNA-1;Parent=Msa0189950;Name=Msa0189950-mRNA-1;_AED=0.14;_eAED=0.14;_QI=364|1|1|1|0.75|0.6|5|223|684
chr2_1 exon 5390845 5390968 5390845 ID=Msa0189950-mRNA-1:exon:2436;Parent=Msa0189950-mRNA-1
chr2_1 exon 5390993 5391345 5390993 ID=Msa0189950-mRNA-1:exon:2437;Parent=Msa0189950-mRNA-1
chr2_1 exon 5391472 5391541 5391472 ID=Msa0189950-mRNA-1:exon:2438;Parent=Msa0189950-mRNA-1
chr2_1 exon 5391833 5393140 5391833 ID=Msa0189950-mRNA-1:exon:2439;Parent=Msa0189950-mRNA-1
chr2_1 exon 5393715 5394501 5393715 ID=Msa0189950-mRNA-1:exon:2440;Parent=Msa0189950-mRNA-1
chr2_1 five_prime_UTR 5390845 5390968 5390845 ID=Msa0189950-mRNA-1:five_prime_utr;Parent=Msa0189950-mRNA-1
chr2_1 five_prime_UTR 5390993 5391232 5390993 ID=Msa0189950-mRNA-1:five_prime_utr;Parent=Msa0189950-mRNA-1
chr2_1 CDS 5391233 5391345 5391233 ID=Msa0189950-mRNA-1:cds;Parent=Msa0189950-mRNA-1
chr2_1 CDS 5391472 5391541 5391472 ID=Msa0189950-mRNA-1:cds;Parent=Msa0189950-mRNA-1
chr2_1 CDS 5391833 5393140 5391833 ID=Msa0189950-mRNA-1:cds;Parent=Msa0189950-mRNA-1
chr2_1 CDS 5393715 5394278 5393715 ID=Msa0189950-mRNA-1:cds;Parent=Msa0189950-mRNA-1
chr2_1 three_prime_UTR 5394279 5394501 5394279 ID=Msa0189950-mRNA-1:three_prime_utr;Parent=Msa0189950-mRNA-1
Gene Sequence

>Msa0189950

ATGTGGGAATCTGAGAGTGATTCAACTGCAAGACACAAGTATGGTGATGGAATTCTCACTTCAAACAAACATGGTGTCAAGACTGAAGGATTTCTTCAAAGAGGTCACTCTTGGTATGTTGCAACTGACGTTCCAAGTGATTTTCTTGTTCAAATTGGAGAAGCTAACTTCCACTTGCATAAGTATCCTCTTATATCTCGAAGTGGAAAGCTGAGTAGAATCATATATGAATCACGCGAACCGGATGTGAATAAGATAGTTATGGATGATATCCCTGGTGGATTTGAGGCATTTGAACTTGCAGCCAAATTCTGCTATGGAATTGCTGTTGATTTAACAGCAGGGAACATTTCTGGACTAAGATGTGCTGCTGAGTATCTAGAAATGACAGAAGATTTAGAAGAAGGCAATCTTATATTCAAAACAGAAGCATTTCTTAGCTATGTTGTTTTATCATCATGGAGAGACTCTATAGTAGTTTTGAAAAGCTGTGAGAAGCTTTCACCTTGGTCAGAGAATCTTCAAATCGTTCGAAGATGCAGCGAGTCTATAGCTTGGAAAGCTTGTGCTAATCCAAAAGGGATAAAATGGTCCTACACAGGAAGAACATCATCAAAAATTTCAAGTCCAAGATGGAATAATAATGATATGAAAGATACAAGTCCAAGTAGGAATCAACTAGTTCCTCCTGATTGGTGGTTTGAAGATGTTTCAATCCTTAGGATTGATCACTTTGTTAGAGTCATTACTGCTATTAAGGTAAAAGGAATGAGATTTGAGTTAATTGGTGCTGCAATAATGCATTATGCAACTAAATGGCTACAAGGGTTGGTTAGTGAAACATCAATCACATTCGATGAAGCGAGCAATTGTGGTAATAGTAATAGTAGTAGTAGTGGTAGCGGTAGTTGGATTAAAGGCGGCGGTGGACTTCACATGATTGTAGCAGGTGGAACTAGAGATGAAACTTCAAGTCTTCAAGCTAAAGAACAAAGGATGATAATTGAGAGTCTTATAAGCATTATTCCTCCTCAGAAAGATACTGTTTCATGCACTTTCCTTCTTAGGCTTCTGAGAATGGCAATTATGTTAAAGGTTGCACCTGCATTGGTTACTGAATTAGAGAAAAGGGTTGGAATGCAATTCGAGCAAGCTACACTTGCTGATCTGTTAATTCCTTGTTATAATAAAGGAGAGACAATGATGTATGATGTTGATCTTGTTATGCGGTTGCTTGAGCATTTTCTTGTACAAGAAGTGACTGAAAATTCTAGTCCGAAAAGACAGTCATTTTCTGAGAAACATATGGGATTGGGATGTAACCTTAATGCTAAAGCAAGAGTGGCAAGGCTTGTTGACAGTTATCTTACAGAGGTTTCAAGAGACAGGAATCTTTCATTGACAAAGTTTCAGGTACTTGCTGAAGCTTTGCCTGAATCAGCTAGAACCTGTGATGATGGACTTTATAGAGCTATTGATTCCTATCTTAAGGCACATCCAACACTTTCTGAGCATGAAAGGAAGCGACTCTGCCGCGTAATGGATTGCCAGAAACTCTCAATTGATGCATGCATGCATGCTGCACAAAATGAAAGGCTTCCATTAAGGGTAGTTGTGCAAGTCCTATTTGCAGAACAGGTAAAGATAAGTACTGCATTATCCAACCCTTCTTTGAAAGATGTTGAATCCTTTACTCAACCAATGGTTACAAACCGAAAAACACTTCTCGAGGCGACACCACAATCATTCCAAGAAGGATGGACAACTGCTAAAAAAGATGTCAACACACTAAAGTTTGAGCTGGAGAGTGTGAAAGCTAAGTATTTGGAGCTTCAAAATGATATGGAAAATTTGCAGAAACAATTTGATAAGATGTTAAAGCAGAAGCATACTTCAGCTTGGAGTAGTGGTTGGAAGAAATTAAGCAAACTTACAAAGATGACAAATGTACAAAATCATGATCATGATCATGATAATTCACCTCAAAGTAATATTCCAGCAGCTCCAGAACAGAACAGAAAAACTACTAGAAGATGGAGAAATTCAATATCTTGA
Protein sequence

>Msa0189950

MWESESDSTARHKYGDGILTSNKHGVKTEGFLQRGHSWYVATDVPSDFLVQIGEANFHLHKYPLISRSGKLSRIIYESREPDVNKIVMDDIPGGFEAFELAAKFCYGIAVDLTAGNISGLRCAAEYLEMTEDLEEGNLIFKTEAFLSYVVLSSWRDSIVVLKSCEKLSPWSENLQIVRRCSESIAWKACANPKGIKWSYTGRTSSKISSPRWNNNDMKDTSPSRNQLVPPDWWFEDVSILRIDHFVRVITAIKVKGMRFELIGAAIMHYATKWLQGLVSETSITFDEASNCGNSNSSSSGSGSWIKGGGGLHMIVAGGTRDETSSLQAKEQRMIIESLISIIPPQKDTVSCTFLLRLLRMAIMLKVAPALVTELEKRVGMQFEQATLADLLIPCYNKGETMMYDVDLVMRLLEHFLVQEVTENSSPKRQSFSEKHMGLGCNLNAKARVARLVDSYLTEVSRDRNLSLTKFQVLAEALPESARTCDDGLYRAIDSYLKAHPTLSEHERKRLCRVMDCQKLSIDACMHAAQNERLPLRVVVQVLFAEQVKISTALSNPSLKDVESFTQPMVTNRKTLLEATPQSFQEGWTTAKKDVNTLKFELESVKAKYLELQNDMENLQKQFDKMLKQKHTSAWSSGWKKLSKLTKMTNVQNHDHDHDNSPQSNIPAAPEQNRKTTRRWRNSIS*