Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0556080 | XP_039689605.1 | 98.895 | 181 | 2 | 0 | 1 | 181 | 1 | 181 | 3.52e-128 | 367 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0556080 | sp|P42856|ZB14_MAIZE | 69.291 | 127 | 39 | 0 | 55 | 181 | 2 | 128 | 1.54e-64 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0556080 | Q2MGQ9 | 98.333 | 180 | 3 | 0 | 2 | 181 | 3 | 182 | 2.27e-127 | 364 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000180 | Msa0556080 | 0.821510 | 3.885086e-53 | -8.615850e-47 |
| Msa0005690 | Msa0556080 | 0.801709 | 7.923134e-49 | -8.615850e-47 |
| Msa0024900 | Msa0556080 | 0.804444 | 2.154369e-49 | -8.615850e-47 |
| Msa0029280 | Msa0556080 | 0.814463 | 1.521053e-51 | -8.615850e-47 |
| Msa0030400 | Msa0556080 | 0.815123 | 1.086147e-51 | -8.615850e-47 |
| Msa0033930 | Msa0556080 | 0.810400 | 1.174578e-50 | -8.615850e-47 |
| Msa0034050 | Msa0556080 | 0.821607 | 3.690972e-53 | -8.615850e-47 |
| Msa0040550 | Msa0556080 | -0.801556 | 8.515528e-49 | -8.615850e-47 |
| Msa0044250 | Msa0556080 | 0.850603 | 1.525934e-60 | -8.615850e-47 |
| Msa0044320 | Msa0556080 | 0.818487 | 1.910586e-52 | -8.615850e-47 |
| Msa0072390 | Msa0556080 | 0.815014 | 1.148491e-51 | -8.615850e-47 |
| Msa0072710 | Msa0556080 | 0.822897 | 1.852119e-53 | -8.615850e-47 |
| Msa0078080 | Msa0556080 | 0.848926 | 4.487562e-60 | -8.615850e-47 |
| Msa0081840 | Msa0556080 | 0.841148 | 5.662647e-58 | -8.615850e-47 |
| Msa0090760 | Msa0556080 | 0.812692 | 3.730013e-51 | -8.615850e-47 |
| Msa0096510 | Msa0556080 | 0.804448 | 2.149750e-49 | -8.615850e-47 |
| Msa0114700 | Msa0556080 | 0.830781 | 2.420895e-55 | -8.615850e-47 |
| Msa0116200 | Msa0556080 | 0.803435 | 3.490483e-49 | -8.615850e-47 |
| Msa0127060 | Msa0556080 | 0.826572 | 2.521064e-54 | -8.615850e-47 |
| Msa0128070 | Msa0556080 | 0.843356 | 1.473354e-58 | -8.615850e-47 |
| Msa0138340 | Msa0556080 | 0.800239 | 1.582283e-48 | -8.615850e-47 |
| Msa0143280 | Msa0556080 | 0.809646 | 1.707494e-50 | -8.615850e-47 |
| Msa0147960 | Msa0556080 | 0.802909 | 4.485602e-49 | -8.615850e-47 |
| Msa0152640 | Msa0556080 | -0.805767 | 1.138926e-49 | -8.615850e-47 |
| Msa0164580 | Msa0556080 | 0.806865 | 6.682799e-50 | -8.615850e-47 |
| Msa0164630 | Msa0556080 | 0.804349 | 2.254783e-49 | -8.615850e-47 |
| Msa0169320 | Msa0556080 | 0.821687 | 3.535962e-53 | -8.615850e-47 |
| Msa0173760 | Msa0556080 | 0.817774 | 2.769475e-52 | -8.615850e-47 |
| Msa0184850 | Msa0556080 | 0.820870 | 5.458337e-53 | -8.615850e-47 |
| Msa0199740 | Msa0556080 | 0.821648 | 3.611358e-53 | -8.615850e-47 |
| Msa0227960 | Msa0556080 | 0.803979 | 2.691964e-49 | -8.615850e-47 |
| Msa0367310 | Msa0556080 | 0.803228 | 3.852646e-49 | -8.615850e-47 |
| Msa0373220 | Msa0556080 | 0.813941 | 1.983566e-51 | -8.615850e-47 |
| Msa0375570 | Msa0556080 | 0.800632 | 1.315698e-48 | -8.615850e-47 |
| Msa0376480 | Msa0556080 | 0.828561 | 8.393638e-55 | -8.615850e-47 |
| Msa0389570 | Msa0556080 | 0.802273 | 6.067227e-49 | -8.615850e-47 |
| Msa0400060 | Msa0556080 | -0.832601 | 8.615303e-56 | -8.615850e-47 |
| Msa0400900 | Msa0556080 | 0.807222 | 5.615290e-50 | -8.615850e-47 |
| Msa0416040 | Msa0556080 | -0.803608 | 3.214475e-49 | -8.615850e-47 |
| Msa0447830 | Msa0556080 | 0.801684 | 8.015231e-49 | -8.615850e-47 |
| Msa0459920 | Msa0556080 | 0.808462 | 3.061346e-50 | -8.615850e-47 |
| Msa0468020 | Msa0556080 | 0.811086 | 8.348335e-51 | -8.615850e-47 |
| Msa0471890 | Msa0556080 | 0.810211 | 1.290141e-50 | -8.615850e-47 |
| Msa0242290 | Msa0556080 | 0.802331 | 5.901363e-49 | -8.615850e-47 |
| Msa0269430 | Msa0556080 | 0.806151 | 9.456206e-50 | -8.615850e-47 |
| Msa0274890 | Msa0556080 | 0.812819 | 3.498494e-51 | -8.615850e-47 |
| Msa0292150 | Msa0556080 | 0.801042 | 1.084940e-48 | -8.615850e-47 |
| Msa0306160 | Msa0556080 | 0.812775 | 3.576977e-51 | -8.615850e-47 |
| Msa0312570 | Msa0556080 | 0.808860 | 2.515988e-50 | -8.615850e-47 |
| Msa0313800 | Msa0556080 | 0.821854 | 3.236285e-53 | -8.615850e-47 |
| Msa0314980 | Msa0556080 | 0.824577 | 7.485810e-54 | -8.615850e-47 |
| Msa0315010 | Msa0556080 | 0.812353 | 4.424720e-51 | -8.615850e-47 |
| Msa0319710 | Msa0556080 | 0.813435 | 2.564054e-51 | -8.615850e-47 |
| Msa0327870 | Msa0556080 | 0.813707 | 2.233044e-51 | -8.615850e-47 |
| Msa0327900 | Msa0556080 | 0.820941 | 5.255241e-53 | -8.615850e-47 |
| Msa0347060 | Msa0556080 | 0.835300 | 1.817793e-56 | -8.615850e-47 |
| Msa0353040 | Msa0556080 | -0.809785 | 1.593329e-50 | -8.615850e-47 |
| Msa0493430 | Msa0556080 | 0.805668 | 1.194710e-49 | -8.615850e-47 |
| Msa0516330 | Msa0556080 | 0.810614 | 1.055934e-50 | -8.615850e-47 |
| Msa0516480 | Msa0556080 | 0.801328 | 9.482365e-49 | -8.615850e-47 |
| Msa0524830 | Msa0556080 | 0.822230 | 2.647153e-53 | -8.615850e-47 |
| Msa0524960 | Msa0556080 | 0.803663 | 3.130492e-49 | -8.615850e-47 |
| Msa0530340 | Msa0556080 | 0.800527 | 1.382349e-48 | -8.615850e-47 |
| Msa0538100 | Msa0556080 | 0.800147 | 1.651914e-48 | -8.615850e-47 |
| Msa0546020 | Msa0556080 | 0.805311 | 1.419225e-49 | -8.615850e-47 |
| Msa0549940 | Msa0556080 | 0.805636 | 1.213384e-49 | -8.615850e-47 |
| Msa0553350 | Msa0556080 | 0.803001 | 4.293790e-49 | -8.615850e-47 |
| Msa0556070 | Msa0556080 | 0.821571 | 3.761333e-53 | -8.615850e-47 |
| Msa0556080 | Msa0589600 | 0.803039 | 4.215514e-49 | -8.615850e-47 |
| Msa0556080 | Msa0618260 | 0.804869 | 1.756210e-49 | -8.615850e-47 |
| Msa0556080 | Msa0618490 | 0.822186 | 2.709819e-53 | -8.615850e-47 |
| Msa0556080 | Msa0625440 | 0.838657 | 2.524289e-57 | -8.615850e-47 |
| Msa0556080 | Msa0633720 | 0.815677 | 8.180026e-52 | -8.615850e-47 |
| Msa0556080 | Msa0644630 | 0.801832 | 7.476027e-49 | -8.615850e-47 |
| Msa0556080 | Msa0651410 | 0.994718 | 1.334689e-209 | -8.615850e-47 |
| Msa0556080 | Msa0677840 | 0.801333 | 9.459870e-49 | -8.615850e-47 |
| Msa0556080 | Msa0690530 | 0.812217 | 4.738153e-51 | -8.615850e-47 |
| Msa0556080 | Msa0703150 | 0.804697 | 1.907796e-49 | -8.615850e-47 |
| Msa0556080 | Msa0707270 | 0.826263 | 2.985655e-54 | -8.615850e-47 |
| Msa0556080 | Msa0750940 | 0.818430 | 1.967937e-52 | -8.615850e-47 |
| Msa0556080 | Msa0791120 | 0.801884 | 7.294825e-49 | -8.615850e-47 |
| Msa0556080 | Msa0821030 | 0.805799 | 1.121422e-49 | -8.615850e-47 |
| Msa0556080 | Msa0832270 | 0.809194 | 2.134962e-50 | -8.615850e-47 |
| Msa0556080 | Msa0846070 | 0.813974 | 1.950136e-51 | -8.615850e-47 |
| Msa0556080 | Msa0884840 | 0.806469 | 8.101564e-50 | -8.615850e-47 |
| Msa0556080 | Msa0917630 | 0.805190 | 1.504351e-49 | -8.615850e-47 |
| Msa0556080 | Msa0967670 | 0.822195 | 2.697491e-53 | -8.615850e-47 |
| Msa0556080 | Msa1014700 | 0.809803 | 1.579392e-50 | -8.615850e-47 |
| Msa0556080 | Msa1024120 | 0.822790 | 1.961594e-53 | -8.615850e-47 |
| Msa0556080 | Msa1029430 | 0.800922 | 1.148204e-48 | -8.615850e-47 |
| Msa0556080 | Msa1038230 | 0.831811 | 1.350774e-55 | -8.615850e-47 |
| Msa0556080 | Msa1044790 | 0.805347 | 1.394928e-49 | -8.615850e-47 |
| Msa0556080 | Msa1056590 | 0.818149 | 2.278670e-52 | -8.615850e-47 |
| Msa0556080 | Msa1067710 | 0.814263 | 1.684256e-51 | -8.615850e-47 |
| Msa0556080 | Msa1068290 | 0.806803 | 6.889068e-50 | -8.615850e-47 |
| Msa0556080 | Msa1073140 | 0.806699 | 7.247610e-50 | -8.615850e-47 |
| Msa0556080 | Msa1107950 | 0.845543 | 3.803215e-59 | -8.615850e-47 |
| Msa0556080 | Msa1120660 | 0.829149 | 6.049451e-55 | -8.615850e-47 |
| Msa0556080 | Msa1144420 | 0.812714 | 3.689093e-51 | -8.615850e-47 |
| Msa0556080 | Msa1145370 | 0.803196 | 3.912393e-49 | -8.615850e-47 |
| Msa0556080 | Msa1157030 | 0.830177 | 3.400358e-55 | -8.615850e-47 |
| Msa0556080 | Msa1164570 | 0.804576 | 2.021318e-49 | -8.615850e-47 |
| Msa0556080 | Msa1173380 | 0.801549 | 8.544918e-49 | -8.615850e-47 |
| Msa0556080 | Msa1179410 | 0.809129 | 2.203704e-50 | -8.615850e-47 |
| Msa0556080 | Msa1181730 | 0.813454 | 2.539298e-51 | -8.615850e-47 |
| Msa0556080 | Msa1183730 | 0.805677 | 1.189251e-49 | -8.615850e-47 |
| Msa0556080 | Msa1191960 | 0.813367 | 2.653738e-51 | -8.615850e-47 |
| Msa0556080 | Msa1193430 | 0.805708 | 1.171678e-49 | -8.615850e-47 |
| Msa0556080 | Msa1205560 | 0.803996 | 2.670426e-49 | -8.615850e-47 |
| Msa0556080 | Msa1221540 | 0.820675 | 6.051289e-53 | -8.615850e-47 |
| Msa0556080 | Msa1223600 | 0.806423 | 8.286037e-50 | -8.615850e-47 |
| Msa0556080 | Msa1226880 | 0.804100 | 2.540500e-49 | -8.615850e-47 |
| Msa0556080 | Msa1230190 | 0.809316 | 2.010011e-50 | -8.615850e-47 |
| Msa0556080 | Msa1264960 | 0.806263 | 8.954052e-50 | -8.615850e-47 |
| Msa0556080 | Msa1272770 | 0.816190 | 6.284003e-52 | -8.615850e-47 |
| Msa0556080 | Msa1327690 | 0.802537 | 5.351834e-49 | -8.615850e-47 |
| Msa0556080 | Msa1334020 | 0.826719 | 2.325285e-54 | -8.615850e-47 |
| Msa0556080 | Msa1341790 | 0.842028 | 3.321075e-58 | -8.615850e-47 |
| Msa0556080 | Msa1342080 | 0.820100 | 8.197102e-53 | -8.615850e-47 |
| Msa0556080 | Msa1349490 | 0.801445 | 8.972772e-49 | -8.615850e-47 |
| Msa0556080 | Msa1351370 | 0.801618 | 8.270945e-49 | -8.615850e-47 |
| Msa0556080 | Msa1356200 | 0.800642 | 1.309730e-48 | -8.615850e-47 |
| Msa0556080 | Msa1372680 | 0.806240 | 9.054965e-50 | -8.615850e-47 |
| Msa0556080 | Msa1386250 | 0.801352 | 9.375982e-49 | -8.615850e-47 |
| Msa0556080 | Msa1393550 | 0.843098 | 1.726647e-58 | -8.615850e-47 |
| Msa0556080 | Msa1395640 | 0.813992 | 1.932753e-51 | -8.615850e-47 |
| Msa0556080 | Msa1400240 | 0.824641 | 7.231362e-54 | -8.615850e-47 |
| Msa0556080 | Msa1413360 | 0.802946 | 4.406475e-49 | -8.615850e-47 |
| Msa0556080 | Msa1418780 | 0.804515 | 2.082122e-49 | -8.615850e-47 |
| Msa0556080 | Msa1426310 | 0.842595 | 2.348773e-58 | -8.615850e-47 |
| Msa0556080 | Msa1431240 | 0.800507 | 1.395091e-48 | -8.615850e-47 |
| Msa0556080 | Msa1432250 | 0.805639 | 1.211170e-49 | -8.615850e-47 |
| Msa0556080 | Msa1438780 | 0.831904 | 1.281233e-55 | -8.615850e-47 |
| Msa0556080 | Msa1449510 | 0.803007 | 4.280218e-49 | -8.615850e-47 |
| Msa0556080 | Msa1450850 | 0.813493 | 2.488891e-51 | -8.615850e-47 |
| Msa0556080 | Msa1451780 | 0.830294 | 3.185131e-55 | -8.615850e-47 |
| Msa0556080 | Msa1462600 | 0.801463 | 8.896689e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0556080 | MtrunA17_Chr4g0043791 | 98.333 | 180 | 3 | 0 | 2 | 181 | 3 | 182 | 4.37e-131 | 364 |
| Msa0556080 | MtrunA17_Chr6g0460311 | 67.647 | 136 | 42 | 1 | 46 | 181 | 20 | 153 | 2.67e-67 | 202 |
| Msa0556080 | MtrunA17_Chr7g0255021 | 53.521 | 71 | 31 | 2 | 53 | 121 | 4 | 74 | 3.24e-17 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0556080 | AT1G31160.1 | 70.225 | 178 | 48 | 2 | 9 | 181 | 10 | 187 | 5.19e-74 | 221 |
| Msa0556080 | AT3G56490.1 | 65.942 | 138 | 43 | 2 | 44 | 181 | 14 | 147 | 6.54e-65 | 196 |
| Msa0556080 | AT1G31160.2 | 64.103 | 117 | 37 | 2 | 9 | 120 | 10 | 126 | 8.68e-31 | 109 |
Find 61 sgRNAs with CRISPR-Local
Find 128 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAATCCATCTCTGAATTT+TGG | 0.116683 | 4_1:-54127959 | None:intergenic |
| TTCTTTCTCGGCTACTATTT+TGG | 0.146644 | 4_1:-54128261 | None:intergenic |
| CGGTTCCTTTCTCTCTTCTT+AGG | 0.195364 | 4_1:+54126358 | Msa0556080:CDS |
| TTACAACCTCACTCGTTTCT+AGG | 0.197311 | 4_1:+54126321 | Msa0556080:five_prime_UTR |
| ATTTATCTTGTCTCTGAAAT+AGG | 0.206802 | 4_1:+54128685 | Msa0556080:three_prime_UTR |
| TTGTTCATTGTTTGCAGCGC+TGG | 0.260695 | 4_1:-54127529 | None:intergenic |
| TGCTAGACATGGGGAGATAC+TGG | 0.286862 | 4_1:+54128220 | Msa0556080:CDS |
| TTTGCAGCGCTGGCGCTAAA+TGG | 0.329280 | 4_1:-54127519 | None:intergenic |
| TGTCAATCCTTGCTGATTCC+TGG | 0.330188 | 4_1:+54128500 | Msa0556080:three_prime_UTR |
| AACCTAAACTTATTCTTTGT+GGG | 0.346148 | 4_1:+54128575 | Msa0556080:three_prime_UTR |
| AGCATTAACAGCAGCTGCTT+TGG | 0.346160 | 4_1:-54127559 | None:intergenic |
| ATTTGACAAGATCATAAATA+AGG | 0.353101 | 4_1:+54127847 | Msa0556080:CDS |
| AAACCTAAACTTATTCTTTG+TGG | 0.382517 | 4_1:+54128574 | Msa0556080:three_prime_UTR |
| ATCAGCGATACCTTCTTTCT+CGG | 0.398686 | 4_1:-54128273 | None:intergenic |
| AACCCACAAAGAATAAGTTT+AGG | 0.405609 | 4_1:-54128577 | None:intergenic |
| ACAGGCTCCGGTTCATGTTC+TGG | 0.408262 | 4_1:+54127931 | Msa0556080:CDS |
| AGAGATGGATTAACAGAACT+TGG | 0.427247 | 4_1:+54127968 | Msa0556080:CDS |
| CATAAAACGAGAGCGAAAAC+TGG | 0.435458 | 4_1:+54128658 | Msa0556080:three_prime_UTR |
| TAGGTGGGAGACAGATGAAT+TGG | 0.451270 | 4_1:+54128464 | Msa0556080:CDS |
| CAGCTATTTCTAAGATCTCC+AGG | 0.451382 | 4_1:-54128518 | None:intergenic |
| TCTTCATATACAATGCTTGA+AGG | 0.456414 | 4_1:-54127875 | None:intergenic |
| TGTCAGGAACTACTCACAAT+TGG | 0.465151 | 4_1:+54126451 | Msa0556080:intron |
| AACTACCTGCGACATTGAAT+AGG | 0.476144 | 4_1:-54126533 | None:intergenic |
| GGAATGACCAGAACATGAAC+CGG | 0.476627 | 4_1:-54127938 | None:intergenic |
| TTGAATAGGAAGAACATAGT+TGG | 0.488856 | 4_1:-54126519 | None:intergenic |
| CTTAAGCAGATAGCTCAGTT+AGG | 0.498510 | 4_1:+54128723 | Msa0556080:three_prime_UTR |
| GTTTAATGTTGATACATTGT+TGG | 0.500439 | 4_1:-54127594 | None:intergenic |
| GGACATCAGTCCACAGGCTC+CGG | 0.500682 | 4_1:+54127919 | Msa0556080:CDS |
| CATTGTATATGAAGATGAAA+AGG | 0.504283 | 4_1:+54127883 | Msa0556080:CDS |
| TTCTTCCTATTCAATGTCGC+AGG | 0.509055 | 4_1:+54126528 | Msa0556080:CDS |
| CATCTGCACTTGCATGTCCT+AGG | 0.526980 | 4_1:+54128445 | Msa0556080:CDS |
| CTTCATATACAATGCTTGAA+GGG | 0.529749 | 4_1:-54127874 | None:intergenic |
| GTCATTCCAAAATTCAGAGA+TGG | 0.530580 | 4_1:+54127953 | Msa0556080:CDS |
| GCTGCTGTTAATGCTGATAG+TGG | 0.535440 | 4_1:+54127567 | Msa0556080:CDS |
| TCTAGGAACCATGGCAGCTA+CGG | 0.537131 | 4_1:+54126338 | Msa0556080:exon |
| CTGCACTTGCATGTCCTAGG+TGG | 0.538200 | 4_1:+54128448 | Msa0556080:CDS |
| TGTTACCTAAGAAGAGAGAA+AGG | 0.545840 | 4_1:-54126363 | None:intergenic |
| AGCTCAGTTAGGTGAGCTGA+GGG | 0.546466 | 4_1:+54128734 | Msa0556080:three_prime_UTR |
| AAAATAGTAGCCGAGAAAGA+AGG | 0.558191 | 4_1:+54128263 | Msa0556080:CDS |
| GAGAAAGAAGGTATCGCTGA+TGG | 0.560682 | 4_1:+54128275 | Msa0556080:CDS |
| GCTAGACATGGGGAGATACT+GGG | 0.563721 | 4_1:+54128221 | Msa0556080:CDS |
| GAAAGGAACCGTAGCTGCCA+TGG | 0.566043 | 4_1:-54126346 | None:intergenic |
| TTTCAGGCTGATGCTAGACA+TGG | 0.567488 | 4_1:+54128209 | Msa0556080:intron |
| AGTGCAGATGATACACACTT+TGG | 0.574867 | 4_1:-54128432 | None:intergenic |
| AGATCTTAGAAATAGCTGAA+TGG | 0.578452 | 4_1:+54128523 | Msa0556080:three_prime_UTR |
| AGACAGATGAATTGGCCACC+TGG | 0.582532 | 4_1:+54128472 | Msa0556080:CDS |
| TAGCTCAGTTAGGTGAGCTG+AGG | 0.584634 | 4_1:+54128733 | Msa0556080:three_prime_UTR |
| GAGATGGATTAACAGAACTT+GGG | 0.585175 | 4_1:+54127969 | Msa0556080:CDS |
| TGCACTTGCATGTCCTAGGT+GGG | 0.590386 | 4_1:+54128449 | Msa0556080:CDS |
| TTCAGGCTGATGCTAGACAT+GGG | 0.598386 | 4_1:+54128210 | Msa0556080:intron |
| GCAAGGATTGACATTCAACC+AGG | 0.602800 | 4_1:-54128490 | None:intergenic |
| GACTGATGTCCCGAAAAGCT+AGG | 0.605770 | 4_1:-54127907 | None:intergenic |
| AATTCATCTGTCTCCCACCT+AGG | 0.607223 | 4_1:-54128462 | None:intergenic |
| AGAACATGAACCGGAGCCTG+TGG | 0.626518 | 4_1:-54127929 | None:intergenic |
| TGGATTAACAGAACTTGGGA+AGG | 0.640825 | 4_1:+54127973 | Msa0556080:CDS |
| TCAGGCTGATGCTAGACATG+GGG | 0.647934 | 4_1:+54128211 | Msa0556080:intron |
| TTTCGTGTTGTCATCAACAG+TGG | 0.675033 | 4_1:+54128299 | Msa0556080:CDS |
| AAGATCTCCAGGAATCAGCA+AGG | 0.677777 | 4_1:-54128507 | None:intergenic |
| TCACTCGTTTCTAGGAACCA+TGG | 0.697647 | 4_1:+54126329 | Msa0556080:exon |
| AGGATTGACATTCAACCAGG+TGG | 0.710391 | 4_1:-54128487 | None:intergenic |
| ATGGTTCCTAGAAACGAGTG+AGG | 0.718741 | 4_1:-54126327 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACACTAATTTATTATATAA+GGG | + | chr4_1:54128053-54128072 | Msa0556080:intron | 10.0% |
| !! | GTACACTAATTTATTATATA+AGG | + | chr4_1:54128052-54128071 | Msa0556080:intron | 15.0% |
| !! | ACACTAATTTATTATATAAG+GGG | + | chr4_1:54128054-54128073 | Msa0556080:intron | 15.0% |
| !!! | TGAATTTTTTATGATCTGTA+AGG | + | chr4_1:54126571-54126590 | Msa0556080:intron | 20.0% |
| !!! | TTTCCAACTTTTATTGTTTT+AGG | - | chr4_1:54126629-54126648 | None:intergenic | 20.0% |
| !! | ATGAAGACAAAAAAGATAAT+GGG | - | chr4_1:54127425-54127444 | None:intergenic | 20.0% |
| !!! | AGATTTAAAGTTTAAATGCA+CGG | + | chr4_1:54127712-54127731 | Msa0556080:intron | 20.0% |
| !! | AATATTGACATTGTAATTCT+AGG | - | chr4_1:54127753-54127772 | None:intergenic | 20.0% |
| !! | ACGAATAAAATAGAAACATT+GGG | - | chr4_1:54127819-54127838 | None:intergenic | 20.0% |
| !! | ATTTGACAAGATCATAAATA+AGG | + | chr4_1:54127847-54127866 | Msa0556080:CDS | 20.0% |
| ! | CTCCAAGTTAAAATAAGTTA+TGG | + | chr4_1:54126854-54126873 | Msa0556080:intron | 25.0% |
| !!! | CTCCATAACTTATTTTAACT+TGG | - | chr4_1:54126859-54126878 | None:intergenic | 25.0% |
| !! | AAAGTAGCTAGAATTGATTT+CGG | - | chr4_1:54126939-54126958 | None:intergenic | 25.0% |
| ! | TTAGTTCAAATGCGAAAAAT+TGG | + | chr4_1:54127006-54127025 | Msa0556080:intron | 25.0% |
| !!! | TGGATAACCTTGTTTTTTAT+TGG | + | chr4_1:54127053-54127072 | Msa0556080:intron | 25.0% |
| ! | AACAAGTTAACAACTTACTT+CGG | - | chr4_1:54127120-54127139 | None:intergenic | 25.0% |
| !! | GTTGAACTTGAACAAATTTT+TGG | - | chr4_1:54127195-54127214 | None:intergenic | 25.0% |
| !!! | CTATTTCTAAAAATGTTGCT+TGG | + | chr4_1:54127250-54127269 | Msa0556080:intron | 25.0% |
| !!! | TAACAAGCTCTAAAAAAACT+TGG | - | chr4_1:54127363-54127382 | None:intergenic | 25.0% |
| ! | CATGAAGACAAAAAAGATAA+TGG | - | chr4_1:54127426-54127445 | None:intergenic | 25.0% |
| !!! | ATCTTTTTTGTCTTCATGTT+TGG | + | chr4_1:54127428-54127447 | Msa0556080:intron | 25.0% |
| ! | GTTTAATGTTGATACATTGT+TGG | - | chr4_1:54127597-54127616 | None:intergenic | 25.0% |
| ! | GACGAATAAAATAGAAACAT+TGG | - | chr4_1:54127820-54127839 | None:intergenic | 25.0% |
| ! | CATTGTATATGAAGATGAAA+AGG | + | chr4_1:54127883-54127902 | Msa0556080:CDS | 25.0% |
| ! | TCTATATGTACATTAACTCA+AGG | + | chr4_1:54128009-54128028 | Msa0556080:intron | 25.0% |
| !!! | TGTTAGCTTTGAGTTATTAT+TGG | + | chr4_1:54128127-54128146 | Msa0556080:intron | 25.0% |
| !!! | GTTAGCTTTGAGTTATTATT+GGG | + | chr4_1:54128128-54128147 | Msa0556080:intron | 25.0% |
| !! | AAAAGAAAATCTGCATTTTC+AGG | + | chr4_1:54128193-54128212 | Msa0556080:intron | 25.0% |
| ! | TGATTAGAAAAAAGACATAC+AGG | - | chr4_1:54128333-54128352 | None:intergenic | 25.0% |
| ! | ACAATTTCCATTGCTAATAA+TGG | + | chr4_1:54128355-54128374 | Msa0556080:intron | 25.0% |
| ! | AATAAGAAAAACGAGATTAG+TGG | - | chr4_1:54128404-54128423 | None:intergenic | 25.0% |
| ! | AAACCTAAACTTATTCTTTG+TGG | + | chr4_1:54128574-54128593 | Msa0556080:three_prime_UTR | 25.0% |
| ! | AACCTAAACTTATTCTTTGT+GGG | + | chr4_1:54128575-54128594 | Msa0556080:three_prime_UTR | 25.0% |
| !!! | ATTTATCTTGTCTCTGAAAT+AGG | + | chr4_1:54128685-54128704 | Msa0556080:three_prime_UTR | 25.0% |
| ! | TCTTGCTTTGAATTATTGTC+AGG | + | chr4_1:54126435-54126454 | Msa0556080:intron | 30.0% |
| TTGAATAGGAAGAACATAGT+TGG | - | chr4_1:54126522-54126541 | None:intergenic | 30.0% | |
| GTGCCTAAAACAATAAAAGT+TGG | + | chr4_1:54126623-54126642 | Msa0556080:intron | 30.0% | |
| AGGTTATCCAAACATGAATT+AGG | - | chr4_1:54127043-54127062 | None:intergenic | 30.0% | |
| ! | AAATTTGTTCAAGTTCAACC+AGG | + | chr4_1:54127196-54127215 | Msa0556080:intron | 30.0% |
| ! | TCTAAAAATGTTGCTTGGAT+CGG | + | chr4_1:54127255-54127274 | Msa0556080:intron | 30.0% |
| ! | ACTCAAAAGATCCTTTTCTT+AGG | - | chr4_1:54127284-54127303 | None:intergenic | 30.0% |
| GTGTGTAACTGCAAATATAA+AGG | + | chr4_1:54127320-54127339 | Msa0556080:intron | 30.0% | |
| !! | TTTAGAGCTTGTTAATAACC+AGG | + | chr4_1:54127369-54127388 | Msa0556080:intron | 30.0% |
| TATAACTTCAAAACAGATCC+TGG | - | chr4_1:54127390-54127409 | None:intergenic | 30.0% | |
| !! | AGTAGCTTTAGTTATTGACT+TGG | + | chr4_1:54127451-54127470 | Msa0556080:intron | 30.0% |
| TGGTGTGAATTCATGTTTAT+TGG | + | chr4_1:54127471-54127490 | Msa0556080:intron | 30.0% | |
| CTGTTAATACAATGCTTGAT+TGG | - | chr4_1:54127789-54127808 | None:intergenic | 30.0% | |
| CTTCATATACAATGCTTGAA+GGG | - | chr4_1:54127877-54127896 | None:intergenic | 30.0% | |
| TCTTCATATACAATGCTTGA+AGG | - | chr4_1:54127878-54127897 | None:intergenic | 30.0% | |
| ! | GTTAATCCATCTCTGAATTT+TGG | - | chr4_1:54127962-54127981 | None:intergenic | 30.0% |
| TTTCCTTACATAGTAGTAAC+AGG | + | chr4_1:54128154-54128173 | Msa0556080:intron | 30.0% | |
| TTCCTTACATAGTAGTAACA+GGG | + | chr4_1:54128155-54128174 | Msa0556080:intron | 30.0% | |
| TTCCATTGCTAATAATGGTA+TGG | + | chr4_1:54128360-54128379 | Msa0556080:intron | 30.0% | |
| TACCATACCATTATTAGCAA+TGG | - | chr4_1:54128365-54128384 | None:intergenic | 30.0% | |
| AGATCTTAGAAATAGCTGAA+TGG | + | chr4_1:54128523-54128542 | Msa0556080:three_prime_UTR | 30.0% | |
| AACCCACAAAGAATAAGTTT+AGG | - | chr4_1:54128580-54128599 | None:intergenic | 30.0% | |
| TGTTACCTAAGAAGAGAGAA+AGG | - | chr4_1:54126366-54126385 | None:intergenic | 35.0% | |
| AATCTGCGTTGTTAATGAGA+TGG | + | chr4_1:54126597-54126616 | Msa0556080:intron | 35.0% | |
| ATCTGCGTTGTTAATGAGAT+GGG | + | chr4_1:54126598-54126617 | Msa0556080:intron | 35.0% | |
| ATTGCATTTCAGTGCATGTT+TGG | + | chr4_1:54126695-54126714 | Msa0556080:intron | 35.0% | |
| !! | CGGAGGTGTAAAATTGATTT+TGG | - | chr4_1:54126919-54126938 | None:intergenic | 35.0% |
| !! | CACATTTCTGAGACTATGTT+TGG | - | chr4_1:54126972-54126991 | None:intergenic | 35.0% |
| TCTGCAACCTAATTCATGTT+TGG | + | chr4_1:54127033-54127052 | Msa0556080:intron | 35.0% | |
| TCAGCTGCCAATAAAAAACA+AGG | - | chr4_1:54127063-54127082 | None:intergenic | 35.0% | |
| AACTTACTTCGGAGAAACAA+AGG | - | chr4_1:54127109-54127128 | None:intergenic | 35.0% | |
| ATTAGACACGATGTAACTAC+TGG | + | chr4_1:54127140-54127159 | Msa0556080:intron | 35.0% | |
| !! | GCTCTAAAAAAACTTGGTGT+TGG | - | chr4_1:54127357-54127376 | None:intergenic | 35.0% |
| ! | GCATTTTGCTTACTTGTTTC+AGG | + | chr4_1:54127493-54127512 | Msa0556080:intron | 35.0% |
| !! | ATGAAAAGGTCCTAGCTTTT+CGG | + | chr4_1:54127897-54127916 | Msa0556080:CDS | 35.0% |
| GTCATTCCAAAATTCAGAGA+TGG | + | chr4_1:54127953-54127972 | Msa0556080:CDS | 35.0% | |
| !! | AGAGATGGATTAACAGAACT+TGG | + | chr4_1:54127968-54127987 | Msa0556080:CDS | 35.0% |
| !! | GAGATGGATTAACAGAACTT+GGG | + | chr4_1:54127969-54127988 | Msa0556080:CDS | 35.0% |
| ! | TTCTTTCTCGGCTACTATTT+TGG | - | chr4_1:54128264-54128283 | None:intergenic | 35.0% |
| AAAATAGTAGCCGAGAAAGA+AGG | + | chr4_1:54128263-54128282 | Msa0556080:CDS | 35.0% | |
| !! | CTCGTTTTTCTTATTCTGCT+AGG | + | chr4_1:54128409-54128428 | Msa0556080:intron | 35.0% |
| TTACAACCTCACTCGTTTCT+AGG | + | chr4_1:54126321-54126340 | Msa0556080:five_prime_UTR | 40.0% | |
| TGTCAGGAACTACTCACAAT+TGG | + | chr4_1:54126451-54126470 | Msa0556080:intron | 40.0% | |
| TTCTTCCTATTCAATGTCGC+AGG | + | chr4_1:54126528-54126547 | Msa0556080:CDS | 40.0% | |
| AACTACCTGCGACATTGAAT+AGG | - | chr4_1:54126536-54126555 | None:intergenic | 40.0% | |
| !!! | TCAGTGCATGTTTGGTTTTG+AGG | + | chr4_1:54126703-54126722 | Msa0556080:intron | 40.0% |
| ! | GTAGCTAGAATTGATTTCGG+AGG | - | chr4_1:54126936-54126955 | None:intergenic | 40.0% |
| TGGATCGGATACCTAAGAAA+AGG | + | chr4_1:54127270-54127289 | Msa0556080:intron | 40.0% | |
| !! | TGAAAAGGTCCTAGCTTTTC+GGG | + | chr4_1:54127898-54127917 | Msa0556080:CDS | 40.0% |
| TGGATTAACAGAACTTGGGA+AGG | + | chr4_1:54127973-54127992 | Msa0556080:CDS | 40.0% | |
| CTCCCTGTTACTACTATGTA+AGG | - | chr4_1:54128160-54128179 | None:intergenic | 40.0% | |
| !! | TTTCTCGGCTACTATTTTGG+CGG | - | chr4_1:54128261-54128280 | None:intergenic | 40.0% |
| ATCAGCGATACCTTCTTTCT+CGG | - | chr4_1:54128276-54128295 | None:intergenic | 40.0% | |
| TTTCGTGTTGTCATCAACAG+TGG | + | chr4_1:54128299-54128318 | Msa0556080:CDS | 40.0% | |
| GAAAAAAGACATACAGGCAC+TGG | - | chr4_1:54128327-54128346 | None:intergenic | 40.0% | |
| AGTGCAGATGATACACACTT+TGG | - | chr4_1:54128435-54128454 | None:intergenic | 40.0% | |
| !! | CAGCTATTTCTAAGATCTCC+AGG | - | chr4_1:54128521-54128540 | None:intergenic | 40.0% |
| ! | CAGTTTTCGCTCTCGTTTTA+TGG | - | chr4_1:54128660-54128679 | None:intergenic | 40.0% |
| CATAAAACGAGAGCGAAAAC+TGG | + | chr4_1:54128658-54128677 | Msa0556080:three_prime_UTR | 40.0% | |
| CTTAAGCAGATAGCTCAGTT+AGG | + | chr4_1:54128723-54128742 | Msa0556080:three_prime_UTR | 40.0% | |
| ATGGTTCCTAGAAACGAGTG+AGG | - | chr4_1:54126330-54126349 | None:intergenic | 45.0% | |
| ! | TCACTCGTTTCTAGGAACCA+TGG | + | chr4_1:54126329-54126348 | Msa0556080:exon | 45.0% |
| CGGTTCCTTTCTCTCTTCTT+AGG | + | chr4_1:54126358-54126377 | Msa0556080:CDS | 45.0% | |
| TTGAGTGTGTCTGCACTGTT+AGG | + | chr4_1:54127298-54127317 | Msa0556080:intron | 45.0% | |
| TTGTTCATTGTTTGCAGCGC+TGG | - | chr4_1:54127532-54127551 | None:intergenic | 45.0% | |
| AGCATTAACAGCAGCTGCTT+TGG | - | chr4_1:54127562-54127581 | None:intergenic | 45.0% | |
| GCTGCTGTTAATGCTGATAG+TGG | + | chr4_1:54127567-54127586 | Msa0556080:CDS | 45.0% | |
| GGAATGACCAGAACATGAAC+CGG | - | chr4_1:54127941-54127960 | None:intergenic | 45.0% | |
| TTTCAGGCTGATGCTAGACA+TGG | + | chr4_1:54128209-54128228 | Msa0556080:intron | 45.0% | |
| TTCAGGCTGATGCTAGACAT+GGG | + | chr4_1:54128210-54128229 | Msa0556080:intron | 45.0% | |
| !! | GAGAAAGAAGGTATCGCTGA+TGG | + | chr4_1:54128275-54128294 | Msa0556080:CDS | 45.0% |
| AATTCATCTGTCTCCCACCT+AGG | - | chr4_1:54128465-54128484 | None:intergenic | 45.0% | |
| TAGGTGGGAGACAGATGAAT+TGG | + | chr4_1:54128464-54128483 | Msa0556080:CDS | 45.0% | |
| AGGATTGACATTCAACCAGG+TGG | - | chr4_1:54128490-54128509 | None:intergenic | 45.0% | |
| GCAAGGATTGACATTCAACC+AGG | - | chr4_1:54128493-54128512 | None:intergenic | 45.0% | |
| !! | TGTCAATCCTTGCTGATTCC+TGG | + | chr4_1:54128500-54128519 | Msa0556080:three_prime_UTR | 45.0% |
| AAGATCTCCAGGAATCAGCA+AGG | - | chr4_1:54128510-54128529 | None:intergenic | 45.0% | |
| TCTAGGAACCATGGCAGCTA+CGG | + | chr4_1:54126338-54126357 | Msa0556080:exon | 50.0% | |
| TCACAGTCAACAACAGCACC+TGG | - | chr4_1:54127217-54127236 | None:intergenic | 50.0% | |
| GACTGATGTCCCGAAAAGCT+AGG | - | chr4_1:54127910-54127929 | None:intergenic | 50.0% | |
| !! | TTTTCGGGACATCAGTCCAC+AGG | + | chr4_1:54127913-54127932 | Msa0556080:CDS | 50.0% |
| TCAGGCTGATGCTAGACATG+GGG | + | chr4_1:54128211-54128230 | Msa0556080:intron | 50.0% | |
| TGCTAGACATGGGGAGATAC+TGG | + | chr4_1:54128220-54128239 | Msa0556080:CDS | 50.0% | |
| GCTAGACATGGGGAGATACT+GGG | + | chr4_1:54128221-54128240 | Msa0556080:CDS | 50.0% | |
| ! | TTTTGGCGGCATAGAGAAGC+TGG | - | chr4_1:54128247-54128266 | None:intergenic | 50.0% |
| CATCTGCACTTGCATGTCCT+AGG | + | chr4_1:54128445-54128464 | Msa0556080:CDS | 50.0% | |
| TGCACTTGCATGTCCTAGGT+GGG | + | chr4_1:54128449-54128468 | Msa0556080:CDS | 50.0% | |
| AGACAGATGAATTGGCCACC+TGG | + | chr4_1:54128472-54128491 | Msa0556080:CDS | 50.0% | |
| GAAAGGAACCGTAGCTGCCA+TGG | - | chr4_1:54126349-54126368 | None:intergenic | 55.0% | |
| !! | TTTGCAGCGCTGGCGCTAAA+TGG | - | chr4_1:54127522-54127541 | None:intergenic | 55.0% |
| AGAACATGAACCGGAGCCTG+TGG | - | chr4_1:54127932-54127951 | None:intergenic | 55.0% | |
| ACAGGCTCCGGTTCATGTTC+TGG | + | chr4_1:54127931-54127950 | Msa0556080:CDS | 55.0% | |
| CTGCACTTGCATGTCCTAGG+TGG | + | chr4_1:54128448-54128467 | Msa0556080:CDS | 55.0% | |
| GGACATCAGTCCACAGGCTC+CGG | + | chr4_1:54127919-54127938 | Msa0556080:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 54126290 | 54128754 | 54126290 | ID=Msa0556080;Name=Msa0556080 |
| chr4_1 | mRNA | 54126290 | 54128754 | 54126290 | ID=Msa0556080-mRNA-1;Parent=Msa0556080;Name=Msa0556080-mRNA-1;_AED=0.03;_eAED=0.03;_QI=58|1|1|1|1|1|6|256|181 |
| chr4_1 | exon | 54126290 | 54126379 | 54126290 | ID=Msa0556080-mRNA-1:exon:15874;Parent=Msa0556080-mRNA-1 |
| chr4_1 | exon | 54126457 | 54126549 | 54126457 | ID=Msa0556080-mRNA-1:exon:15875;Parent=Msa0556080-mRNA-1 |
| chr4_1 | exon | 54127515 | 54127601 | 54127515 | ID=Msa0556080-mRNA-1:exon:15876;Parent=Msa0556080-mRNA-1 |
| chr4_1 | exon | 54127847 | 54127994 | 54127847 | ID=Msa0556080-mRNA-1:exon:15877;Parent=Msa0556080-mRNA-1 |
| chr4_1 | exon | 54128215 | 54128332 | 54128215 | ID=Msa0556080-mRNA-1:exon:15878;Parent=Msa0556080-mRNA-1 |
| chr4_1 | exon | 54128431 | 54128754 | 54128431 | ID=Msa0556080-mRNA-1:exon:15879;Parent=Msa0556080-mRNA-1 |
| chr4_1 | five_prime_UTR | 54126290 | 54126347 | 54126290 | ID=Msa0556080-mRNA-1:five_prime_utr;Parent=Msa0556080-mRNA-1 |
| chr4_1 | CDS | 54126348 | 54126379 | 54126348 | ID=Msa0556080-mRNA-1:cds;Parent=Msa0556080-mRNA-1 |
| chr4_1 | CDS | 54126457 | 54126549 | 54126457 | ID=Msa0556080-mRNA-1:cds;Parent=Msa0556080-mRNA-1 |
| chr4_1 | CDS | 54127515 | 54127601 | 54127515 | ID=Msa0556080-mRNA-1:cds;Parent=Msa0556080-mRNA-1 |
| chr4_1 | CDS | 54127847 | 54127994 | 54127847 | ID=Msa0556080-mRNA-1:cds;Parent=Msa0556080-mRNA-1 |
| chr4_1 | CDS | 54128215 | 54128332 | 54128215 | ID=Msa0556080-mRNA-1:cds;Parent=Msa0556080-mRNA-1 |
| chr4_1 | CDS | 54128431 | 54128498 | 54128431 | ID=Msa0556080-mRNA-1:cds;Parent=Msa0556080-mRNA-1 |
| chr4_1 | three_prime_UTR | 54128499 | 54128754 | 54128499 | ID=Msa0556080-mRNA-1:three_prime_utr;Parent=Msa0556080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
