AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0589600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589600 MtrunA17_Chr4g0006851 94.488 381 20 1 1 381 1 380 0.0 701
Msa0589600 MtrunA17_Chr7g0242311 76.115 381 82 3 1 378 1 375 0.0 567
Msa0589600 MtrunA17_Chr4g0063561 35.211 284 166 6 90 363 123 398 9.78e-52 177
Msa0589600 MtrunA17_Chr4g0063561 30.857 175 114 4 189 357 122 295 1.16e-15 77.8
Msa0589600 MtrunA17_Chr7g0240341 33.898 295 175 7 91 365 26 320 1.20e-48 167
Msa0589600 MtrunA17_Chr1g0149111 35.915 284 156 7 90 359 139 410 2.27e-48 169
Msa0589600 MtrunA17_Chr1g0149111 27.011 174 123 1 189 358 138 311 1.51e-16 80.9
Msa0589600 MtrunA17_Chr3g0137941 35.315 286 163 7 92 365 124 399 3.37e-47 165
Msa0589600 MtrunA17_Chr3g0137941 34.031 191 105 6 88 268 214 393 8.77e-19 87.0
Msa0589600 MtrunA17_Chr3g0137941 26.012 173 124 1 189 357 121 293 1.09e-15 78.2
Msa0589600 MtrunA17_Chr8g0347451 31.715 309 168 6 90 360 51 354 9.83e-46 160
Msa0589600 MtrunA17_Chr3g0135861 32.803 314 174 5 83 365 28 335 1.56e-45 159
Msa0589600 MtrunA17_Chr3g0135861 26.190 210 133 4 90 278 131 339 1.28e-15 77.4
Msa0589600 MtrunA17_Chr5g0396801 32.248 307 167 6 90 360 38 339 8.79e-45 157
Msa0589600 MtrunA17_Chr6g0488081 31.746 315 161 7 78 356 7 303 1.35e-42 151
Msa0589600 MtrunA17_Chr3g0131501 30.921 304 177 8 77 357 185 478 3.28e-42 154
Msa0589600 MtrunA17_Chr3g0131501 31.053 190 118 3 87 268 296 480 5.91e-18 85.1
Msa0589600 MtrunA17_Chr8g0385571 32.155 283 168 7 90 357 227 500 2.73e-40 149
Msa0589600 MtrunA17_Chr8g0385571 29.474 190 121 3 87 268 318 502 1.56e-18 87.0
Msa0589600 MtrunA17_Chr2g0296061 28.713 303 176 6 92 354 17 319 1.67e-39 143
Msa0589600 MtrunA17_Chr2g0296061 27.273 209 126 4 191 373 16 224 1.30e-16 80.1
Msa0589600 MtrunA17_Chr2g0296061 24.510 204 122 4 90 268 128 324 1.94e-13 70.9
Msa0589600 MtrunA17_Chr5g0436041 34.483 290 170 7 90 360 34 322 8.80e-39 141
Msa0589600 MtrunA17_Chr5g0436041 30.890 191 114 6 90 270 141 323 7.17e-11 62.8
Msa0589600 MtrunA17_Chr4g0067231 28.065 310 181 6 92 359 16 325 1.83e-38 140
Msa0589600 MtrunA17_Chr4g0067231 28.302 212 123 5 191 373 15 226 1.87e-18 85.5
Msa0589600 MtrunA17_Chr4g0067231 26.214 206 116 6 90 268 129 325 1.89e-14 73.6
Msa0589600 MtrunA17_Chr7g0252401 32.270 282 166 9 90 357 207 477 3.07e-38 143
Msa0589600 MtrunA17_Chr7g0252401 30.769 182 118 2 90 268 303 479 1.59e-21 95.9
Msa0589600 MtrunA17_Chr7g0252401 26.404 178 122 3 190 358 207 384 1.63e-11 65.5
Msa0589600 MtrunA17_Chr3g0135801 32.646 291 172 8 90 359 34 321 2.11e-37 137
Msa0589600 MtrunA17_Chr3g0115801 35.034 294 151 10 92 356 118 400 6.12e-33 127
Msa0589600 MtrunA17_Chr3g0115801 27.083 192 134 3 189 375 115 305 2.81e-14 73.9
Msa0589600 MtrunA17_Chr8g0393031 30.420 286 175 10 95 358 20 303 7.61e-32 122
Msa0589600 MtrunA17_Chr8g0382201 32.313 294 159 9 92 356 197 479 4.90e-31 123
Msa0589600 MtrunA17_Chr8g0382201 27.041 196 131 3 173 356 170 365 7.27e-13 69.7
Msa0589600 MtrunA17_Chr7g0269701 30.000 290 178 10 92 361 24 308 9.37e-27 108
Msa0589600 MtrunA17_Chr7g0269701 29.032 186 115 5 191 360 23 207 2.57e-15 76.3
Msa0589600 MtrunA17_Chr7g0269701 28.177 181 111 6 104 272 137 310 5.24e-11 63.2
Msa0589600 MtrunA17_Chr8g0352031 31.894 301 179 9 76 353 52 349 3.51e-26 107
Msa0589600 MtrunA17_Chr6g0488341 28.854 253 139 5 135 365 4 237 4.57e-26 105
Msa0589600 MtrunA17_Chr4g0000261 26.263 297 183 8 90 359 66 353 5.18e-26 107
Msa0589600 MtrunA17_Chr4g0000261 25.521 192 122 2 187 357 63 254 7.42e-15 75.1
Msa0589600 MtrunA17_Chr3g0088301 31.379 290 176 8 90 359 93 379 1.71e-25 106
Msa0589600 MtrunA17_Chr4g0040341 32.746 284 168 9 90 353 75 355 2.14e-24 103
Msa0589600 MtrunA17_Chr2g0328891 30.530 321 191 11 59 359 44 352 8.34e-23 98.6
Msa0589600 MtrunA17_Chr1g0195101 26.882 279 181 9 102 361 31 305 2.12e-21 93.6
Msa0589600 MtrunA17_Chr4g0008061 24.399 291 197 7 90 366 15 296 1.54e-20 90.9
Msa0589600 MtrunA17_Chr5g0431531 26.855 283 189 6 89 359 38 314 2.01e-20 91.3
Msa0589600 MtrunA17_Chr7g0241521 23.711 291 199 7 90 366 17 298 2.41e-20 90.5
Msa0589600 MtrunA17_Chr8g0372531 26.667 285 179 9 106 364 30 310 6.07e-20 89.4
Msa0589600 MtrunA17_Chr5g0423111 25.168 298 190 8 102 378 33 318 8.66e-20 89.7
Msa0589600 MtrunA17_Chr8g0386871 25.714 280 186 7 91 360 321 588 1.56e-18 87.4
Msa0589600 MtrunA17_Chr8g0386871 26.842 190 120 7 192 369 322 504 7.87e-13 69.7
Msa0589600 MtrunA17_Chr8g0383321 24.910 277 178 7 91 360 53 306 5.36e-18 84.0
Msa0589600 MtrunA17_Chr8g0383321 29.787 188 110 5 92 271 135 308 6.19e-15 75.1
Msa0589600 MtrunA17_Chr6g0463041 27.753 227 108 5 190 365 25 246 8.29e-18 82.0
Msa0589600 MtrunA17_Chr6g0463041 26.897 145 98 3 131 268 97 240 9.28e-12 64.7
Msa0589600 MtrunA17_Chr1g0201361 24.910 277 178 7 91 360 52 305 2.45e-17 82.0
Msa0589600 MtrunA17_Chr1g0201361 30.108 186 114 5 92 272 134 308 1.38e-13 70.9
Msa0589600 MtrunA17_Chr3g0086811 23.948 309 187 8 90 357 28 329 1.26e-16 80.5
Msa0589600 MtrunA17_Chr4g0028531 22.034 295 201 8 91 366 23 307 8.60e-16 77.4
Msa0589600 MtrunA17_Chr3g0103681 24.621 264 181 8 90 337 15 276 1.05e-15 77.0
Msa0589600 MtrunA17_Chr3g0133111 24.262 305 196 7 90 359 6 310 2.69e-15 75.9
Msa0589600 MtrunA17_Chr3g0133111 26.596 188 128 4 92 270 126 312 3.44e-12 66.6
Msa0589600 MtrunA17_Chr5g0411411 25.517 290 193 8 92 360 11 298 4.49e-15 75.1
Msa0589600 MtrunA17_Chr5g0411411 26.667 210 138 5 70 268 93 297 3.05e-12 66.6
Msa0589600 MtrunA17_Chr8g0343381 27.083 192 113 4 190 359 35 221 5.75e-14 72.4
Msa0589600 MtrunA17_Chr8g0366791 29.630 189 123 3 90 268 109 297 1.39e-13 70.9
Msa0589600 MtrunA17_Chr8g0366791 25.743 303 191 11 83 360 5 298 1.46e-13 70.9
Msa0589600 MtrunA17_Chr6g0463351 25.758 330 207 10 28 341 16 323 5.19e-13 69.7
Msa0589600 MtrunA17_Chr1g0196921 19.863 292 202 7 94 362 18 300 9.98e-13 68.2
Msa0589600 MtrunA17_Chr1g0196921 23.370 184 133 2 91 266 112 295 2.95e-12 67.0
Msa0589600 MtrunA17_Chr2g0293621 25.850 294 183 10 93 358 32 318 1.44e-12 68.2
Msa0589600 MtrunA17_Chr5g0412401 22.930 314 198 8 90 359 6 319 3.74e-12 66.6
Msa0589600 MtrunA17_Chr3g0136041 26.531 196 119 3 190 362 34 227 4.47e-12 66.6
Msa0589600 MtrunA17_Chr4g0004571 23.973 292 193 8 90 363 16 296 5.08e-12 66.2
Msa0589600 MtrunA17_Chr1g0200171 28.276 145 102 2 134 276 648 792 6.00e-12 67.4
Msa0589600 MtrunA17_Chr1g0200171 21.384 318 219 10 61 364 489 789 5.73e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589600 AT3G51870.1 68.267 375 114 2 6 376 4 377 0.0 511
Msa0589600 AT5G01500.1 77.301 326 74 0 51 376 80 405 1.05e-174 493
Msa0589600 AT4G26180.1 32.822 326 185 5 78 375 9 328 3.21e-48 166
Msa0589600 AT4G32400.1 33.798 287 170 6 90 365 114 391 6.25e-48 167
Msa0589600 AT4G32400.1 31.000 200 120 5 83 274 202 391 1.51e-19 89.7
Msa0589600 AT4G32400.1 28.177 181 126 1 189 365 113 293 3.81e-18 85.5
Msa0589600 AT4G01100.1 32.039 309 167 6 90 360 42 345 5.57e-47 164
Msa0589600 AT4G01100.1 31.100 209 111 4 92 273 147 349 3.87e-21 94.0
Msa0589600 AT2G37890.1 33.910 289 171 6 90 359 45 332 5.91e-47 163
Msa0589600 AT2G37890.1 33.161 193 110 7 90 270 152 337 4.96e-16 79.0
Msa0589600 AT3G55640.1 35.906 298 157 8 85 356 33 322 1.77e-46 162
Msa0589600 AT3G55640.4 35.906 298 157 8 85 356 33 322 1.77e-46 162
Msa0589600 AT4G01100.2 30.650 323 167 6 90 360 42 359 8.21e-45 158
Msa0589600 AT4G01100.2 31.100 209 111 4 92 273 161 363 4.88e-21 94.0
Msa0589600 AT4G01100.3 31.438 299 162 6 100 360 2 295 8.52e-44 154
Msa0589600 AT4G01100.3 31.100 209 111 4 92 273 97 299 1.46e-21 94.4
Msa0589600 AT3G53940.1 32.990 291 173 7 90 360 73 361 1.70e-43 155
Msa0589600 AT4G26180.2 33.333 288 161 4 78 340 9 290 1.17e-42 150
Msa0589600 AT1G14560.1 32.203 295 164 4 101 365 38 326 8.61e-42 149
Msa0589600 AT5G61810.2 32.155 283 168 5 89 360 64 333 7.31e-41 147
Msa0589600 AT5G61810.1 31.930 285 170 5 87 360 205 476 1.28e-39 147
Msa0589600 AT5G48970.1 28.852 305 174 6 92 353 24 328 3.54e-39 143
Msa0589600 AT5G48970.1 30.928 194 109 4 191 359 23 216 1.68e-20 92.0
Msa0589600 AT5G48970.1 23.558 208 122 5 90 268 135 334 3.06e-12 67.4
Msa0589600 AT1G14560.2 32.963 270 145 4 101 340 38 301 2.19e-38 140
Msa0589600 AT3G21390.1 29.524 315 176 7 92 362 21 333 2.81e-37 137
Msa0589600 AT3G21390.1 28.500 200 120 5 191 367 20 219 8.90e-18 84.0
Msa0589600 AT3G21390.1 27.228 202 113 4 90 263 130 325 9.20e-13 68.9
Msa0589600 AT5G51050.1 33.108 296 155 11 85 357 218 493 4.33e-37 140
Msa0589600 AT5G51050.1 30.769 182 119 3 90 268 318 495 7.38e-19 88.6
Msa0589600 AT3G21390.2 29.524 315 176 7 92 362 43 355 6.38e-37 137
Msa0589600 AT3G21390.2 28.500 200 120 5 191 367 42 241 1.70e-17 83.6
Msa0589600 AT3G21390.2 27.228 202 113 4 90 263 152 347 1.15e-12 68.9
Msa0589600 AT3G20240.1 31.169 308 176 8 82 362 44 342 9.84e-36 134
Msa0589600 AT3G20240.1 24.510 204 128 3 174 356 41 239 7.34e-12 66.6
Msa0589600 AT5G07320.1 31.250 272 163 6 100 360 219 477 7.22e-34 131
Msa0589600 AT5G56450.1 30.508 295 177 8 90 359 34 325 1.42e-33 127
Msa0589600 AT5G56450.1 28.395 162 105 4 88 242 143 300 6.44e-11 63.5
Msa0589600 AT3G55640.2 37.629 194 105 4 85 265 33 223 2.49e-33 125
Msa0589600 AT3G55640.3 37.629 194 105 4 85 265 33 223 2.49e-33 125
Msa0589600 AT5G48970.2 27.626 257 151 5 133 354 2 258 5.55e-31 119
Msa0589600 AT5G48970.2 31.724 145 91 3 223 359 1 145 6.51e-16 77.4
Msa0589600 AT5G48970.2 23.558 208 122 5 90 268 64 263 2.68e-12 67.0
Msa0589600 AT5G64970.1 31.419 296 159 10 92 356 137 419 7.55e-31 122
Msa0589600 AT5G64970.1 28.652 178 121 3 184 356 129 305 3.08e-12 68.2
Msa0589600 AT3G53940.2 35.106 188 108 4 90 265 73 258 2.69e-30 118
Msa0589600 AT3G53940.2 28.780 205 121 6 174 356 57 258 6.60e-13 68.9
Msa0589600 AT5G64970.2 30.303 297 163 10 91 356 12 295 5.45e-30 117
Msa0589600 AT5G64970.3 31.429 280 148 10 92 340 137 403 2.78e-27 112
Msa0589600 AT5G64970.3 28.652 178 121 3 184 356 129 305 2.73e-12 68.2
Msa0589600 AT1G78180.1 31.633 294 161 9 92 356 130 412 2.44e-26 109
Msa0589600 AT1G78180.1 29.651 172 117 2 189 356 127 298 2.81e-12 68.2
Msa0589600 AT5G17400.1 29.773 309 180 13 77 358 1 299 1.30e-25 105
Msa0589600 AT1G14560.3 30.088 226 128 3 170 365 3 228 1.62e-25 103
Msa0589600 AT1G14560.3 31.500 200 114 5 90 268 25 222 4.45e-18 83.2
Msa0589600 AT2G30160.1 27.900 319 203 8 61 359 10 321 1.66e-25 105
Msa0589600 AT2G30160.1 22.816 206 155 1 176 377 28 233 1.59e-14 74.3
Msa0589600 AT3G08580.2 28.793 323 200 9 64 359 52 371 6.77e-25 105
Msa0589600 AT3G08580.1 28.793 323 200 9 64 359 52 371 6.77e-25 105
Msa0589600 AT1G25380.1 27.636 275 179 5 102 358 33 305 8.78e-25 104
Msa0589600 AT1G25380.1 29.545 176 105 6 201 360 33 205 1.33e-13 72.0
Msa0589600 AT1G25380.2 27.636 275 179 5 102 358 33 305 8.78e-25 104
Msa0589600 AT1G25380.2 29.545 176 105 6 201 360 33 205 1.33e-13 72.0
Msa0589600 AT5G13490.1 29.655 290 182 7 90 359 88 375 1.84e-24 103
Msa0589600 AT5G13490.2 29.655 290 182 7 90 359 88 375 1.84e-24 103
Msa0589600 AT1G07030.1 27.744 328 207 9 45 359 6 316 1.02e-23 100
Msa0589600 AT4G28390.1 30.450 289 180 8 90 359 83 369 1.45e-23 101
Msa0589600 AT4G28390.2 30.450 289 180 8 90 359 83 369 1.45e-23 101
Msa0589600 AT2G47490.1 29.455 275 174 8 102 359 29 300 7.54e-23 98.2
Msa0589600 AT2G47490.1 28.000 175 109 6 201 360 29 201 8.84e-11 62.8
Msa0589600 AT3G54110.1 24.904 261 183 5 90 337 15 275 7.07e-21 92.4
Msa0589600 AT4G39460.2 27.957 279 169 8 90 360 58 312 3.45e-20 90.9
Msa0589600 AT4G39460.2 29.817 218 121 7 65 271 118 314 7.17e-16 78.2
Msa0589600 AT4G39460.1 27.957 279 169 8 90 360 58 312 3.45e-20 90.9
Msa0589600 AT4G39460.1 29.817 218 121 7 65 271 118 314 7.17e-16 78.2
Msa0589600 AT4G39460.3 27.957 279 169 8 90 360 58 312 3.45e-20 90.9
Msa0589600 AT4G39460.3 29.817 218 121 7 65 271 118 314 7.17e-16 78.2
Msa0589600 AT5G66380.1 29.010 293 179 11 92 359 12 300 1.23e-19 89.0
Msa0589600 AT1G34065.1 25.779 353 216 11 27 365 21 341 1.25e-19 89.4
Msa0589600 AT4G03115.3 26.119 268 186 6 73 333 53 315 1.88e-19 89.0
Msa0589600 AT4G03115.1 26.119 268 186 6 73 333 21 283 1.94e-19 88.6
Msa0589600 AT1G34065.2 27.660 282 176 8 90 365 75 334 2.86e-19 88.2
Msa0589600 AT5G58970.1 24.382 283 199 6 90 358 16 297 3.74e-19 87.4
Msa0589600 AT4G11440.1 25.614 285 186 9 91 360 331 604 1.67e-16 81.6
Msa0589600 AT4G11440.2 25.614 285 186 9 91 360 331 604 1.67e-16 81.6
Msa0589600 AT4G03115.5 26.852 216 146 5 125 333 2 212 1.97e-16 78.6
Msa0589600 AT1G14140.1 24.407 295 192 10 91 362 17 303 1.18e-15 77.4
Msa0589600 AT4G03115.6 26.066 211 148 4 130 333 11 220 1.40e-15 76.3
Msa0589600 AT5G46800.2 26.298 289 187 9 92 356 10 296 1.82e-15 76.6
Msa0589600 AT5G46800.2 27.979 193 124 4 90 268 108 299 4.31e-13 69.7
Msa0589600 AT5G46800.3 26.298 289 187 9 92 356 10 296 1.82e-15 76.6
Msa0589600 AT5G46800.3 27.979 193 124 4 90 268 108 299 4.31e-13 69.7
Msa0589600 AT5G46800.1 26.298 289 187 9 92 356 10 296 1.82e-15 76.6
Msa0589600 AT5G46800.1 27.979 193 124 4 90 268 108 299 4.31e-13 69.7
Msa0589600 AT4G03115.4 28.141 199 138 3 73 271 21 214 3.82e-15 74.7
Msa0589600 AT4G03115.2 28.877 187 128 3 85 271 33 214 4.56e-15 74.3
Msa0589600 AT5G01340.1 23.611 288 193 8 91 358 18 298 7.15e-15 75.1
Msa0589600 AT5G26200.1 25.379 264 159 7 139 364 79 342 1.08e-13 72.0
Msa0589600 AT5G26200.1 26.263 198 122 5 190 365 41 236 2.64e-11 64.7
Msa0589600 AT4G24570.1 23.607 305 198 5 90 359 6 310 7.06e-13 69.3
Msa0589600 AT5G58970.2 24.242 231 161 5 90 306 16 246 4.38e-12 66.2
Msa0589600 AT2G22500.1 23.841 302 197 7 90 358 6 307 7.52e-12 66.2
Msa0589600 AT2G35800.1 21.223 278 200 7 91 363 546 809 9.67e-12 67.0
Msa0589600 AT1G72820.1 27.273 187 117 4 190 359 33 217 1.06e-11 65.9
Msa0589600 AT1G72820.2 27.273 187 117 4 190 359 33 217 1.06e-11 65.9
Msa0589600 AT1G79900.1 22.422 223 166 2 119 334 36 258 6.38e-11 63.2
Msa0589600 AT5G42130.1 26.623 154 100 2 139 279 251 404 9.16e-11 63.5

Find 125 sgRNAs with CRISPR-Local

Find 390 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGATTTGTGTCAACTTAT+TGG 0.153600 4_2:-12700573 Msa0589600:three_prime_UTR
AAGTTCACCATCATTGATTA+TGG 0.166015 4_2:+12702264 None:intergenic
GTTAGAGGTGGTGGTTAAAT+TGG 0.220101 4_2:+12706639 None:intergenic
GGTGGAGACGGTGGCGAATT+TGG 0.220482 4_2:+12706540 None:intergenic
TTCCTTCCCTATCATTGATA+TGG 0.235134 4_2:+12704055 None:intergenic
GTGGCACAAGATGGAGTTAT+TGG 0.246853 4_2:-12702721 Msa0589600:CDS
TAGGATAAGTTTGATGGTTT+TGG 0.254529 4_2:+12706713 None:intergenic
ACAGTATTAGATGCCATTTC+AGG 0.261132 4_2:-12702994 Msa0589600:intron
AGACTTCTCCTATGGCTATT+AGG 0.261404 4_2:-12700543 Msa0589600:three_prime_UTR
GTAGGACTCAAGTTTGTTCT+AGG 0.268017 4_2:-12700678 Msa0589600:three_prime_UTR
GAGAAACAATGCAGCATCTT+TGG 0.271353 4_2:+12706435 None:intergenic
GGCCTTGGTCCTTCTCTTAT+CGG 0.280665 4_2:-12703302 Msa0589600:CDS
GGGAAGGAAGAAGGCATTAA+AGG 0.294715 4_2:-12704041 Msa0589600:CDS
GCATCTAATACTGTCTTATA+AGG 0.297227 4_2:+12703003 None:intergenic
CTTTGCTTATGAAATTTATA+AGG 0.302156 4_2:-12703874 Msa0589600:intron
TTCGTTGTTCGGTTTGGTTT+GGG 0.312231 4_2:+12706516 None:intergenic
CGCGAATTCGTTGTTCGGTT+TGG 0.326585 4_2:+12706510 None:intergenic
ATTCGTTGTTCGGTTTGGTT+TGG 0.334224 4_2:+12706515 None:intergenic
AAGAAGGCATTAAAGGTTAC+TGG 0.339670 4_2:-12704034 Msa0589600:CDS
TTAGAGGTGGTGGTTAAATT+GGG 0.348256 4_2:+12706640 None:intergenic
CAAAAGTTAACTGCAATATA+TGG 0.353568 4_2:+12703272 None:intergenic
GCTTCTTTCTACTACGGCCT+TGG 0.361517 4_2:-12703317 Msa0589600:CDS
AGAAACAATGCAGCATCTTT+GGG 0.370149 4_2:+12706436 None:intergenic
AAAGCAATTCCCACAACTAA+TGG 0.376764 4_2:+12700749 None:intergenic
CAGACTCGTGGAGTGCGAAT+TGG 0.377216 4_2:-12706257 Msa0589600:CDS
TAAGGATAGGATAAGTTTGA+TGG 0.379841 4_2:+12706707 None:intergenic
TTGCAGGCCATATCAATGAT+AGG 0.383329 4_2:-12704062 Msa0589600:intron
AAGAGCTGAACAGCACTATA+AGG 0.384778 4_2:+12703894 None:intergenic
TTTCTCGCCGGTGCTGTTGC+CGG 0.386588 4_2:-12706418 Msa0589600:CDS
ATATCTCACTGCTAGCAAAT+TGG 0.395642 4_2:+12700724 None:intergenic
AACGATCAGCTCTCTGTTGT+CGG 0.407429 4_2:-12703753 Msa0589600:CDS
GAAAGTGCTAAGAAGACAAT+TGG 0.415198 4_2:-12706230 Msa0589600:CDS
GAGTTGTTGAAGAGGTGGTT+GGG 0.416337 4_2:+12706604 None:intergenic
TTCGTTCTACTGCGTGTGTT+TGG 0.418357 4_2:+12706740 None:intergenic
CAATGCCAACTAACCTGAAA+TGG 0.419802 4_2:+12702981 None:intergenic
GGAGTTGTTGAAGAGGTGGT+TGG 0.421605 4_2:+12706603 None:intergenic
AGCAGCTCCGGCAACAGCAC+CGG 0.424640 4_2:+12706411 None:intergenic
GTTCGGTTTGGTTTGGGAGG+TGG 0.426742 4_2:+12706522 None:intergenic
CGTAATCTCAGGACATCTAA+AGG 0.428560 4_2:+12703484 None:intergenic
TTACGATTAGCTGTTGAACT+TGG 0.435908 4_2:-12703466 Msa0589600:CDS
ATTACAGCTAAAATACATTC+AGG 0.438813 4_2:+12700630 None:intergenic
AGTTCACCATCATTGATTAT+GGG 0.439080 4_2:+12702265 None:intergenic
CCTTCTCACTAGCAGCAATC+AGG 0.448175 4_2:+12702334 None:intergenic
GTCGGCGCGAATTCGTTGTT+CGG 0.453231 4_2:+12706505 None:intergenic
TAAAATTATCCATTAGTTGT+GGG 0.456350 4_2:-12700758 Msa0589600:three_prime_UTR
ACAAAAGCGACGACGGCGAT+CGG 0.456914 4_2:+12706460 None:intergenic
GATCACAAGTACCTGCTGTT+TGG 0.457445 4_2:+12702658 None:intergenic
TTTGGTTTGGGAGGTGGAGA+CGG 0.464583 4_2:+12706528 None:intergenic
CAAAAGCGACGACGGCGATC+GGG 0.464792 4_2:+12706461 None:intergenic
GGGTGAAACGACGTCGTTAG+AGG 0.465059 4_2:+12706624 None:intergenic
GTTGAGTTTGTCATAAGGAT+AGG 0.477976 4_2:+12706694 None:intergenic
GGCATTTCTTTAGTTTGTTG+CGG 0.498494 4_2:+12702286 None:intergenic
TGTTCGTTGAGTTTGTCATA+AGG 0.498708 4_2:+12706689 None:intergenic
GTAATCTCAGGACATCTAAA+GGG 0.501170 4_2:+12703485 None:intergenic
AAATTTACAGAAAATATTCA+AGG 0.503522 4_2:-12703781 Msa0589600:intron
ATAAAATTATCCATTAGTTG+TGG 0.515167 4_2:-12700759 Msa0589600:three_prime_UTR
TTTGGAAGGAATGGTGGTGG+TGG 0.522252 4_2:+12706558 None:intergenic
GTATGAACAGACTTCTCCTA+TGG 0.522328 4_2:-12700551 Msa0589600:three_prime_UTR
CGCCTTGAGCATGGTAAGAG+AGG 0.526243 4_2:-12703348 Msa0589600:CDS
TAATCAATGATGGTGAACTT+AGG 0.527251 4_2:-12702261 Msa0589600:three_prime_UTR
AACGACGTCGTTAGAGGTGG+TGG 0.529351 4_2:+12706630 None:intergenic
TACGATTAGCTGTTGAACTT+GGG 0.529803 4_2:-12703465 Msa0589600:CDS
GAAGACAATTGGTTTCATTG+AGG 0.535119 4_2:-12706219 Msa0589600:intron
TCGAATCAAACTTCTCATGC+AGG 0.539543 4_2:-12706362 Msa0589600:intron
GAAATGCCCATAATCAATGA+TGG 0.541338 4_2:-12702271 Msa0589600:exon
AGAAGTTTGATTCGATCAAG+TGG 0.545093 4_2:+12706370 None:intergenic
AAGCATTGGGCACAAATCCT+CGG 0.546969 4_2:+12702692 None:intergenic
ACAAGTACCTGCTGTTTGGT+AGG 0.550522 4_2:+12702662 None:intergenic
GATATTGTGCCACGTGGCGA+CGG 0.551954 4_2:+12706663 None:intergenic
CAACAGCTAATCGTAATCTC+AGG 0.552872 4_2:+12703473 None:intergenic
TCGATCAAGTGGAGCAGTGA+CGG 0.556672 4_2:+12706381 None:intergenic
TGCTATTCACACATTAGAGA+AGG 0.557012 4_2:+12706785 None:intergenic
ATTCACACATTAGAGAAGGC+TGG 0.558582 4_2:+12706789 None:intergenic
GGCATTAAAGGTTACTGGAA+GGG 0.558590 4_2:-12704029 Msa0589600:CDS
GGGTGGTTAAGAAGCTGAGC+AGG 0.560734 4_2:+12706481 None:intergenic
CACACTTACATGCTATCCTC+TGG 0.561081 4_2:-12703061 Msa0589600:CDS
TTTATAGGTCGCCTTGAGCA+TGG 0.566530 4_2:-12703357 Msa0589600:intron
TAGAGGTGGTGGTTAAATTG+GGG 0.567609 4_2:+12706641 None:intergenic
TTGAGCATGGTAAGAGAGGA+AGG 0.567910 4_2:-12703344 Msa0589600:CDS
AATTTACAGAAAATATTCAA+GGG 0.571080 4_2:-12703780 Msa0589600:intron
GGTGGCGAATTTGGAAGGAA+TGG 0.574574 4_2:+12706549 None:intergenic
GGCAAGAGATGCTGAAAGCA+CGG 0.575833 4_2:+12703083 None:intergenic
TGAAGGTGGAGTTGTTGAAG+AGG 0.577683 4_2:+12706596 None:intergenic
GGTTTGGGAGGTGGAGACGG+TGG 0.578467 4_2:+12706531 None:intergenic
AATGGTGGTGGTGGTTGCGT+CGG 0.578955 4_2:+12706567 None:intergenic
GGATAAAACCTAATAGCCAT+AGG 0.580175 4_2:+12700535 None:intergenic
TGCAGGCCATATCAATGATA+GGG 0.580409 4_2:-12704061 Msa0589600:intron
GAGACGGTGGCGAATTTGGA+AGG 0.581708 4_2:+12706544 None:intergenic
GGAGTTATTGGCTTGTACCG+AGG 0.582134 4_2:-12702709 Msa0589600:CDS
GTTGTCGGAAGACTTGCAGC+TGG 0.582635 4_2:-12703738 Msa0589600:CDS
GATGCTGCATTGTTTCTCGC+CGG 0.586437 4_2:-12706430 Msa0589600:CDS
GCAGGTATTGTGGCACAAGA+TGG 0.586976 4_2:-12702730 Msa0589600:intron
TCAATGATAGGGAAGGAAGA+AGG 0.587858 4_2:-12704050 Msa0589600:CDS
AAGCGACGACGGCGATCGGG+TGG 0.589692 4_2:+12706464 None:intergenic
AGGTGGAGTTGTTGAAGAGG+TGG 0.589863 4_2:+12706599 None:intergenic
GGCCATATCAATGATAGGGA+AGG 0.590764 4_2:-12704057 Msa0589600:CDS
AGGCATTAAAGGTTACTGGA+AGG 0.593539 4_2:-12704030 Msa0589600:CDS
CCTGATTGCTGCTAGTGAGA+AGG 0.594558 4_2:-12702334 Msa0589600:CDS
TATGATTGATGCAGGTATTG+TGG 0.595736 4_2:-12702740 Msa0589600:intron
TAGCAGTGAGATATCTCAGA+AGG 0.596118 4_2:-12700715 Msa0589600:three_prime_UTR
GGAGTTTCAAACAATTGCAG+AGG 0.597624 4_2:-12702313 Msa0589600:CDS
TCGCAGACAGATGCAACTGA+AGG 0.601278 4_2:-12703031 Msa0589600:CDS
TTCCTCTCTTACCATGCTCA+AGG 0.602242 4_2:+12703346 None:intergenic
GTTGTTCGGTTTGGTTTGGG+AGG 0.603199 4_2:+12706519 None:intergenic
TTAAGAAGCTGAGCAGGCGT+CGG 0.604877 4_2:+12706487 None:intergenic
CGCAGACAGATGCAACTGAA+GGG 0.605798 4_2:-12703030 Msa0589600:CDS
TAGTGATGCTCACCTGTGGT+AGG 0.606895 4_2:+12704003 None:intergenic
GGAGCTATTCCGATAAGAGA+AGG 0.610783 4_2:+12703293 None:intergenic
GGCGAATTTGGAAGGAATGG+TGG 0.612416 4_2:+12706552 None:intergenic
AGCAGTGAGATATCTCAGAA+GGG 0.619251 4_2:-12700714 Msa0589600:three_prime_UTR
GTTTGGTTATGCTCACATGA+AGG 0.623240 4_2:+12706757 None:intergenic
GAATTTGGAAGGAATGGTGG+TGG 0.624286 4_2:+12706555 None:intergenic
CTGGAAGGGTAACCTACCAC+AGG 0.641606 4_2:-12704015 Msa0589600:intron
CAGAGGATAGCATGTAAGTG+TGG 0.643661 4_2:+12703062 None:intergenic
GCAGTGAGATATCTCAGAAG+GGG 0.651708 4_2:-12700713 Msa0589600:three_prime_UTR
TTTGGGCACAAAAGCGACGA+CGG 0.653114 4_2:+12706453 None:intergenic
TGAAACGACGTCGTTAGAGG+TGG 0.660799 4_2:+12706627 None:intergenic
GGTTGCGTCGGTGAAAGTGA+AGG 0.665657 4_2:+12706579 None:intergenic
TGCGTCGGTGAAAGTGAAGG+TGG 0.665833 4_2:+12706582 None:intergenic
TTCCGATAAGAGAAGGACCA+AGG 0.667681 4_2:+12703300 None:intergenic
CGAACAAAACCGTCGCCACG+TGG 0.668933 4_2:-12706672 Msa0589600:CDS
TGTCTGCGAACTGTGTCCAG+AGG 0.695566 4_2:+12703045 None:intergenic
ATTGGGGATATTGTGCCACG+TGG 0.728063 4_2:+12706657 None:intergenic
TGGGTGTCGAACAATGACAG+AGG 0.760125 4_2:-12703446 Msa0589600:intron
AAGAGATGCTGAAAGCACGG+CGG 0.775581 4_2:+12703086 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATATTTTATAATAATTA+TGG - chr4_2:12705554-12705573 Msa0589600:intron 0.0%
!! TTTAATAATATAATATGATG+TGG + chr4_2:12705278-12705297 None:intergenic 10.0%
!!! GATTTTTTTAAAAAAAAATC+TGG - chr4_2:12705461-12705480 Msa0589600:intron 10.0%
!! TTTATACAAAGAAAAAAATT+TGG + chr4_2:12705502-12705521 None:intergenic 10.0%
!! ATTAAAATATTGTAAAAAGT+TGG + chr4_2:12705529-12705548 None:intergenic 10.0%
!!! TTTTTAGAAAAATAAATTTC+TGG + chr4_2:12705622-12705641 None:intergenic 10.0%
!! TAAAAAGATTTAAAAAATTC+CGG - chr4_2:12705728-12705747 Msa0589600:intron 10.0%
!!! AATTTACTTTTTTTGTATTT+TGG + chr4_2:12705764-12705783 None:intergenic 10.0%
!!! ATTTTTTTTATCAAAAATTC+TGG - chr4_2:12705805-12705824 Msa0589600:intron 10.0%
!!! AAATTTAATTTTTGAAAATC+TGG - chr4_2:12705833-12705852 Msa0589600:intron 10.0%
!!! TTTTCGATTTTTAAATTTTT+TGG - chr4_2:12706139-12706158 Msa0589600:intron 10.0%
!!! TTTTCGATTTTTTTAATTTT+TGG - chr4_2:12706224-12706243 Msa0589600:CDS 10.0%
!!! TGAATATTTTTATGATTTTT+TGG - chr4_2:12706255-12706274 Msa0589600:CDS 10.0%
!! TTTATTGCTAAAATAATTAA+GGG + chr4_2:12706544-12706563 None:intergenic 10.0%
!!! TTTTATTGCTAAAATAATTA+AGG + chr4_2:12706545-12706564 None:intergenic 10.0%
!!! TTTTTTTTTTGATTACAAAG+AGG - chr4_2:12701419-12701438 Msa0589600:intron 15.0%
!!! TTTTTTTTTGATTACAAAGA+GGG - chr4_2:12701420-12701439 Msa0589600:intron 15.0%
!! ACTTAATATTATGAGTTTAA+TGG - chr4_2:12701901-12701920 Msa0589600:intron 15.0%
!!! AATAAATTGATTTTTGAGTA+GGG + chr4_2:12702838-12702857 None:intergenic 15.0%
!!! AATAAATTGATTTTTGAGTA+GGG - chr4_2:12702838-12702857 Msa0589600:intron 15.0%
!! TTAAAATAAAACATGTGAAT+AGG + chr4_2:12702864-12702883 None:intergenic 15.0%
!!! AATTATTTTAGTTCTGTAAT+TGG - chr4_2:12702992-12703011 Msa0589600:intron 15.0%
!!! GGATTTTATATTTTTTTTGT+TGG - chr4_2:12703100-12703119 Msa0589600:CDS 15.0%
!! AACATTACCTATAAATTTAT+TGG - chr4_2:12703215-12703234 Msa0589600:intron 15.0%
!! AAATTTACAGAAAATATTCA+AGG - chr4_2:12703537-12703556 Msa0589600:intron 15.0%
!! AATTTACAGAAAATATTCAA+GGG - chr4_2:12703538-12703557 Msa0589600:intron 15.0%
!!! TTATTTTTTTGCAAATTTAC+TGG + chr4_2:12704778-12704797 None:intergenic 15.0%
!! TAAATTTGCAAAAAAATAAG+TGG - chr4_2:12704779-12704798 Msa0589600:intron 15.0%
!!! ATGATATATTTAGTTGATAA+TGG - chr4_2:12704875-12704894 Msa0589600:intron 15.0%
!! TTAGGATTCATATTAATTAT+TGG - chr4_2:12705075-12705094 Msa0589600:intron 15.0%
!! TAGGATTCATATTAATTATT+GGG - chr4_2:12705076-12705095 Msa0589600:intron 15.0%
!!! GAAAAAAAATTTTTTTACGA+GGG + chr4_2:12705178-12705197 None:intergenic 15.0%
!!! TCGTAAAAAAATTTTTTTTC+CGG - chr4_2:12705178-12705197 Msa0589600:intron 15.0%
!!! TAAATTTCTGGAAAAATTTT+AGG + chr4_2:12705610-12705629 None:intergenic 15.0%
!!! AATTTTCAAAATTACAAAAG+AGG + chr4_2:12706026-12706045 None:intergenic 15.0%
!!! AAAAAGATTTTAAAAGATTC+TGG + chr4_2:12706054-12706073 None:intergenic 15.0%
!! ATATGATATCATGTCAATTA+AGG + chr4_2:12701366-12701385 None:intergenic 20.0%
!! AAAACAAATATTTGTATGCA+AGG - chr4_2:12701552-12701571 Msa0589600:intron 20.0%
!!! AGACAATGAAAAACTATTTT+GGG + chr4_2:12701866-12701885 None:intergenic 20.0%
!! AAATGAATATGTCAACTTAT+CGG + chr4_2:12701971-12701990 None:intergenic 20.0%
!! AAGTTGACATATTCATTTAA+TGG - chr4_2:12701973-12701992 Msa0589600:intron 20.0%
!!! TAATGATTATTTTACAGTGT+CGG - chr4_2:12702003-12702022 Msa0589600:intron 20.0%
!!! TAAAAAGAACATTTTCATCA+TGG - chr4_2:12702286-12702305 Msa0589600:CDS 20.0%
!!! AAAAAGAACATTTTCATCAT+GGG - chr4_2:12702287-12702306 Msa0589600:CDS 20.0%
!!! CAATAAATTGATTTTTGAGT+AGG + chr4_2:12702837-12702856 None:intergenic 20.0%
!!! CAATAAATTGATTTTTGAGT+AGG - chr4_2:12702837-12702856 Msa0589600:intron 20.0%
!! AAATATAAAATCCTTGATCA+GGG + chr4_2:12703093-12703112 None:intergenic 20.0%
!! AAAATATAAAATCCTTGATC+AGG + chr4_2:12703094-12703113 None:intergenic 20.0%
!! CTTTGCTTATGAAATTTATA+AGG - chr4_2:12703444-12703463 Msa0589600:intron 20.0%
!! AAATATTTGTAAACCATACA+AGG + chr4_2:12703662-12703681 None:intergenic 20.0%
!! ATTAATATTATGATTGATGC+AGG - chr4_2:12704570-12704589 Msa0589600:intron 20.0%
!! ATCAGCAAAATTAGAAATTA+AGG + chr4_2:12704730-12704749 None:intergenic 20.0%
!!! GGAAAAAAAATTTTTTTACG+AGG + chr4_2:12705179-12705198 None:intergenic 20.0%
!! TAAAAATTAGCAAAAAGTTC+TGG - chr4_2:12705423-12705442 Msa0589600:intron 20.0%
!!! GTTCTGGAATTTTTTTTTTA+TGG - chr4_2:12705439-12705458 Msa0589600:intron 20.0%
!!! GTATTTTGGAAATTAAATTC+CGG + chr4_2:12705750-12705769 None:intergenic 20.0%
!! ATAAAATTATCCATTAGTTG+TGG - chr4_2:12706559-12706578 Msa0589600:CDS 20.0%
!! TAAAATTATCCATTAGTTGT+GGG - chr4_2:12706560-12706579 Msa0589600:CDS 20.0%
! GTTACACATACATATAATTG+AGG - chr4_2:12701292-12701311 Msa0589600:intron 25.0%
! TTGACATGATATCATATCAT+TGG - chr4_2:12701369-12701388 Msa0589600:intron 25.0%
!!! AATAAGATTGGAAGTATCAT+GGG + chr4_2:12701694-12701713 None:intergenic 25.0%
!!! AATGAAAAACTATTTTGGGA+AGG + chr4_2:12701862-12701881 None:intergenic 25.0%
!! CAGACAATGAAAAACTATTT+TGG + chr4_2:12701867-12701886 None:intergenic 25.0%
!! GGAATTTGTTTATACCATTT+CGG - chr4_2:12702119-12702138 Msa0589600:intron 25.0%
!!! GTGTTCTAAAATGAATGTAA+TGG + chr4_2:12702197-12702216 None:intergenic 25.0%
! AATTGAAGACTTCATTTGAT+CGG + chr4_2:12702389-12702408 None:intergenic 25.0%
!! ATTCTAAAAGACACAATCAT+TGG - chr4_2:12702679-12702698 Msa0589600:CDS 25.0%
! TGTACACTAAACAGTTAAAT+TGG - chr4_2:12702733-12702752 Msa0589600:intron 25.0%
!!! ACATCTATTTTTATCTCAAC+CGG + chr4_2:12702781-12702800 None:intergenic 25.0%
! CTAAAATAATTACTAACCAG+AGG + chr4_2:12702983-12703002 None:intergenic 25.0%
! GAAAACTACATGAAAATTGA+TGG + chr4_2:12703050-12703069 None:intergenic 25.0%
! AATATAAAATCCTTGATCAG+GGG + chr4_2:12703092-12703111 None:intergenic 25.0%
! GTACTAAATGTGTAAGTTAA+TGG - chr4_2:12703122-12703141 Msa0589600:CDS 25.0%
!!! TAGTTTTTAGCGATATTAGA+TGG - chr4_2:12703166-12703185 Msa0589600:intron 25.0%
! GTAAGCTCCAATAAATTTAT+AGG + chr4_2:12703225-12703244 None:intergenic 25.0%
! AAAGACTACTCAACATTATA+AGG + chr4_2:12703362-12703381 None:intergenic 25.0%
!!! TTGTTTTAGTGTTTCTTTTC+AGG - chr4_2:12703387-12703406 Msa0589600:intron 25.0%
! AATTCTTGAAAGATAGTTAG+CGG + chr4_2:12703474-12703493 None:intergenic 25.0%
! ATTAAAACGGGAAAGATATA+AGG + chr4_2:12703925-12703944 None:intergenic 25.0%
! ACAATTCTGTTCATTAAAAC+GGG + chr4_2:12703937-12703956 None:intergenic 25.0%
! CACAATTCTGTTCATTAAAA+CGG + chr4_2:12703938-12703957 None:intergenic 25.0%
! CAAAAGTTAACTGCAATATA+TGG + chr4_2:12704049-12704068 None:intergenic 25.0%
!!! CTTTATCTTTTTAAGTGCAT+TGG - chr4_2:12704100-12704119 Msa0589600:intron 25.0%
! TTTAATCTATTACCCATACA+AGG + chr4_2:12704162-12704181 None:intergenic 25.0%
! AGCAAAATTAGAAATTAAGG+AGG + chr4_2:12704727-12704746 None:intergenic 25.0%
!! TCAAACCGAAAATTTTACAA+GGG + chr4_2:12706471-12706490 None:intergenic 25.0%
!! ATCAAACCGAAAATTTTACA+AGG + chr4_2:12706470-12706489 None:intergenic 25.0%
! AAATCTGGATACAAATTTAG+AGG - chr4_2:12705848-12705867 Msa0589600:intron 25.0%
! AATCTGGATACAAATTTAGA+GGG - chr4_2:12705849-12705868 Msa0589600:intron 25.0%
!! ATCAAACCGAAAATTTTACA+AGG - chr4_2:12706470-12706489 Msa0589600:CDS 25.0%
!! TCAAACCGAAAATTTTACAA+GGG - chr4_2:12706471-12706490 Msa0589600:CDS 25.0%
! ATTACAGCTAAAATACATTC+AGG + chr4_2:12706691-12706710 None:intergenic 25.0%
!! AATGATTTGTGTCAACTTAT+TGG - chr4_2:12706745-12706764 Msa0589600:five_prime_UTR 25.0%
!! TAGGATAAGTTTGATGGTTT+TGG + chr4_2:12700608-12700627 None:intergenic 30.0%
TAAGGATAGGATAAGTTTGA+TGG + chr4_2:12700614-12700633 None:intergenic 30.0%
AGTTTATTGATATACACCGT+CGG - chr4_2:12701136-12701155 Msa0589600:intron 30.0%
TTCAACCACATTAAATGATG+TGG + chr4_2:12701323-12701342 None:intergenic 30.0%
ACTCTTATCGAGAAACAAAT+CGG + chr4_2:12701609-12701628 None:intergenic 30.0%
!! CAATAAGATTGGAAGTATCA+TGG + chr4_2:12701695-12701714 None:intergenic 30.0%
GTTTAATGGAAGTAAAGTGA+CGG - chr4_2:12701915-12701934 Msa0589600:intron 30.0%
!! ATCGGTTTTACTTCAATATG+TGG + chr4_2:12701953-12701972 None:intergenic 30.0%
! TAACAACTGTTTCTCATTAG+TGG - chr4_2:12702084-12702103 Msa0589600:intron 30.0%
GTTTCTCATTAGTGGTTAAT+TGG - chr4_2:12702092-12702111 Msa0589600:intron 30.0%
TTGAATAGGAAATACCGAAA+TGG + chr4_2:12702136-12702155 None:intergenic 30.0%
! TCAAGTAACGCATTTTGAAT+AGG + chr4_2:12702150-12702169 None:intergenic 30.0%
! CTCTTTCTTTTCTATTACTC+AGG + chr4_2:12702458-12702477 None:intergenic 30.0%
TGGAGTTACAATAATACACA+AGG - chr4_2:12702753-12702772 Msa0589600:intron 30.0%
GGAGTTACAATAATACACAA+GGG - chr4_2:12702754-12702773 Msa0589600:intron 30.0%
GGTTGAGATAAAAATAGATG+TGG - chr4_2:12702780-12702799 Msa0589600:intron 30.0%
AAACTATTGTCAAGGAGTTT+GGG + chr4_2:12703152-12703171 None:intergenic 30.0%
AAAACTATTGTCAAGGAGTT+TGG + chr4_2:12703153-12703172 None:intergenic 30.0%
ATCGCTAAAAACTATTGTCA+AGG + chr4_2:12703160-12703179 None:intergenic 30.0%
! TAGCGATATTAGATGGTTTT+TGG - chr4_2:12703173-12703192 Msa0589600:intron 30.0%
!!! TGTTTCTTTTCAGGTGATTA+GGG - chr4_2:12703396-12703415 Msa0589600:intron 30.0%
TTTGTAAACCATACAAGGAT+AGG + chr4_2:12703657-12703676 None:intergenic 30.0%
AGTAAGATACATTGACATCA+AGG + chr4_2:12703787-12703806 None:intergenic 30.0%
!! CGTTTTAATGAACAGAATTG+TGG - chr4_2:12703936-12703955 Msa0589600:intron 30.0%
! AACAGAATTGTGGCTTTTAT+AGG - chr4_2:12703946-12703965 Msa0589600:intron 30.0%
!!! TTTTTAAGTGCATTGGTGTT+TGG - chr4_2:12704107-12704126 Msa0589600:intron 30.0%
! GTTCTCTTTTGATATTTCTC+TGG + chr4_2:12704195-12704214 None:intergenic 30.0%
GCATCTAATACTGTCTTATA+AGG + chr4_2:12704318-12704337 None:intergenic 30.0%
GCACAGAAAATGATTCTAAA+AGG + chr4_2:12704373-12704392 None:intergenic 30.0%
TTGTTGCTATAACAGCTAAA+AGG + chr4_2:12704467-12704486 None:intergenic 30.0%
TATATGTAAGCACACATTGA+TGG - chr4_2:12704501-12704520 Msa0589600:intron 30.0%
!!! GGTAGGTTTTTTAAAGCATT+GGG + chr4_2:12704642-12704661 None:intergenic 30.0%
!!! TGGTAGGTTTTTTAAAGCAT+TGG + chr4_2:12704643-12704662 None:intergenic 30.0%
AATTAGAAATTAAGGAGGCA+TGG + chr4_2:12704722-12704741 None:intergenic 30.0%
GGATAAAATCCTGTTGAAAT+CGG - chr4_2:12704800-12704819 Msa0589600:intron 30.0%
! AACAAAAAACCGATTTCAAC+AGG + chr4_2:12704812-12704831 None:intergenic 30.0%
TTGTGTGTTCTTAGTAAGTT+AGG + chr4_2:12704848-12704867 None:intergenic 30.0%
! AGTTCACCATCATTGATTAT+GGG + chr4_2:12705056-12705075 None:intergenic 30.0%
! AAGTTCACCATCATTGATTA+TGG + chr4_2:12705057-12705076 None:intergenic 30.0%
! TAATCAATGATGGTGAACTT+AGG - chr4_2:12705057-12705076 Msa0589600:intron 30.0%
! TTTCGCCCTTGTAAAATTTT+CGG - chr4_2:12705141-12705160 Msa0589600:intron 30.0%
! GTGCTTAATTCTAGGAAAAA+TGG + chr4_2:12705897-12705916 None:intergenic 30.0%
!!! TGTGAAATTTTTTTGACACG+TGG - chr4_2:12706332-12706351 Msa0589600:intron 30.0%
!!! TCCGGAATTTTTTTTTTACG+AGG - chr4_2:12706441-12706460 Msa0589600:CDS 30.0%
CCCTCGTAAAAAAAAAATTC+CGG + chr4_2:12706445-12706464 None:intergenic 30.0%
!!! CCGGAATTTTTTTTTTACGA+GGG - chr4_2:12706442-12706461 Msa0589600:CDS 30.0%
! TTTCGCCCTTGTAAAATTTT+CGG + chr4_2:12705141-12705160 None:intergenic 30.0%
!! CTATTAGGTTTTATCCCTTT+GGG - chr4_2:12706790-12706809 Msa0589600:five_prime_UTR 30.0%
TGCTATTCACACATTAGAGA+AGG + chr4_2:12700536-12700555 None:intergenic 35.0%
GTTGAGTTTGTCATAAGGAT+AGG + chr4_2:12700627-12700646 None:intergenic 35.0%
TGTTCGTTGAGTTTGTCATA+AGG + chr4_2:12700632-12700651 None:intergenic 35.0%
! TTAGAGGTGGTGGTTAAATT+GGG + chr4_2:12700681-12700700 None:intergenic 35.0%
AGAAACAATGCAGCATCTTT+GGG + chr4_2:12700885-12700904 None:intergenic 35.0%
! AGAAGTTTGATTCGATCAAG+TGG + chr4_2:12700951-12700970 None:intergenic 35.0%
!! GAAAGTGCTAAGAAGACAAT+TGG - chr4_2:12701088-12701107 Msa0589600:intron 35.0%
! GAAGACAATTGGTTTCATTG+AGG - chr4_2:12701099-12701118 Msa0589600:intron 35.0%
TGATGCCACATCATTTAATG+TGG - chr4_2:12701315-12701334 Msa0589600:intron 35.0%
TTGGTTATAGCTGATGCTTT+GGG + chr4_2:12701502-12701521 None:intergenic 35.0%
TTTGGTTATAGCTGATGCTT+TGG + chr4_2:12701503-12701522 None:intergenic 35.0%
ACCAAATGACTAAACTGAGT+CGG - chr4_2:12701517-12701536 Msa0589600:intron 35.0%
! TTCCAATCTTATTGCTGTTG+AGG - chr4_2:12701701-12701720 Msa0589600:intron 35.0%
ATCCTCAACAGCAATAAGAT+TGG + chr4_2:12701706-12701725 None:intergenic 35.0%
! AAGGTTTTGCGAAGAGAATT+GGG + chr4_2:12701843-12701862 None:intergenic 35.0%
!!! TATTTTACAGTGTCGGTGTT+TGG - chr4_2:12702010-12702029 Msa0589600:intron 35.0%
!!! ATTTTACAGTGTCGGTGTTT+GGG - chr4_2:12702011-12702030 Msa0589600:intron 35.0%
CATTAGTGGTTAATTGGTTG+TGG - chr4_2:12702098-12702117 Msa0589600:intron 35.0%
TGGTTACATGATGTCAAAGA+TGG + chr4_2:12702330-12702349 None:intergenic 35.0%
! GCAAGTTGAAGAGATCAAAA+TGG - chr4_2:12702588-12702607 Msa0589600:intron 35.0%
TTGAGTCAGCTATTATCTGA+CGG + chr4_2:12702630-12702649 None:intergenic 35.0%
AGACACAATCATTGGATGTT+AGG - chr4_2:12702687-12702706 Msa0589600:CDS 35.0%
TACAATAATACACAAGGGTC+CGG - chr4_2:12702759-12702778 Msa0589600:intron 35.0%
!! AAAATAGATGTGGCTCTAGA+AGG - chr4_2:12702790-12702809 Msa0589600:intron 35.0%
TAATCCAGTTTGCAGTCTAA+AGG - chr4_2:12703015-12703034 Msa0589600:CDS 35.0%
AATCCAGTTTGCAGTCTAAA+GGG - chr4_2:12703016-12703035 Msa0589600:CDS 35.0%
AACTATTGTCAAGGAGTTTG+GGG + chr4_2:12703151-12703170 None:intergenic 35.0%
TTCCTTCCCTATCATTGATA+TGG + chr4_2:12703266-12703285 None:intergenic 35.0%
!! AAGAAGGCATTAAAGGTTAC+TGG - chr4_2:12703284-12703303 Msa0589600:CDS 35.0%
!!! GTGTTTCTTTTCAGGTGATT+AGG - chr4_2:12703395-12703414 Msa0589600:intron 35.0%
TTATGAGACCTATCCTTGTA+TGG - chr4_2:12703646-12703665 Msa0589600:intron 35.0%
GTAATCTCAGGACATCTAAA+GGG + chr4_2:12703836-12703855 None:intergenic 35.0%
! TTACGATTAGCTGTTGAACT+TGG - chr4_2:12703852-12703871 Msa0589600:intron 35.0%
! TACGATTAGCTGTTGAACTT+GGG - chr4_2:12703853-12703872 Msa0589600:intron 35.0%
! AACAATGACAGAGGTATATG+TGG - chr4_2:12703881-12703900 Msa0589600:CDS 35.0%
! ACAATGACAGAGGTATATGT+GGG - chr4_2:12703882-12703901 Msa0589600:CDS 35.0%
! GGTTTTGCTTCTTTCTACTA+CGG - chr4_2:12703995-12704014 Msa0589600:intron 35.0%
TAAGACATCCTAACCTTGTA+TGG - chr4_2:12704146-12704165 Msa0589600:intron 35.0%
AAGACATCCTAACCTTGTAT+GGG - chr4_2:12704147-12704166 Msa0589600:intron 35.0%
TCTATTACCCATACAAGGTT+AGG + chr4_2:12704157-12704176 None:intergenic 35.0%
! ACAGTATTAGATGCCATTTC+AGG - chr4_2:12704324-12704343 Msa0589600:intron 35.0%
! GCAAGTTTTGCACAGATAAA+GGG + chr4_2:12704438-12704457 None:intergenic 35.0%
! TATGATTGATGCAGGTATTG+TGG - chr4_2:12704578-12704597 Msa0589600:intron 35.0%
TAAAAAACCTACCAAACAGC+AGG - chr4_2:12704649-12704668 Msa0589600:intron 35.0%
!! GGCATTTCTTTAGTTTGTTG+CGG + chr4_2:12705035-12705054 None:intergenic 35.0%
GAAATGCCCATAATCAATGA+TGG - chr4_2:12705047-12705066 Msa0589600:intron 35.0%
! GTTTTTCACCCCTGTAATAT+AGG - chr4_2:12705107-12705126 Msa0589600:intron 35.0%
! AATTTTACAAGGGCGAAAAC+CGG + chr4_2:12706481-12706500 None:intergenic 35.0%
!! AGAGGGTTTTATAAAAGTCG+TGG - chr4_2:12705866-12705885 Msa0589600:intron 35.0%
!! CTAAGGAAGTGCTTAATTCT+AGG + chr4_2:12705905-12705924 None:intergenic 35.0%
AGAATTAAGCACTTCCTTAG+AGG - chr4_2:12705905-12705924 Msa0589600:intron 35.0%
TATGATTTCTACCTCCTCTA+AGG + chr4_2:12705922-12705941 None:intergenic 35.0%
! AATTTTACAAGGGCGAAAAC+CGG - chr4_2:12706481-12706500 Msa0589600:CDS 35.0%
CCTGTAATATAGGTCATTTC+CGG + chr4_2:12706503-12706522 None:intergenic 35.0%
CGGAAATGACCTATATTACA+GGG - chr4_2:12706501-12706520 Msa0589600:CDS 35.0%
GGAAATGACCTATATTACAG+GGG - chr4_2:12706502-12706521 Msa0589600:CDS 35.0%
! GTTTTTCACCCCTGTAATAT+AGG + chr4_2:12705107-12705126 None:intergenic 35.0%
AAAGCAATTCCCACAACTAA+TGG + chr4_2:12706572-12706591 None:intergenic 35.0%
ATATCTCACTGCTAGCAAAT+TGG + chr4_2:12706597-12706616 None:intergenic 35.0%
! AAGGGGACTGTAAATTTTGT+AGG - chr4_2:12706622-12706641 Msa0589600:CDS 35.0%
GGATAAAACCTAATAGCCAT+AGG + chr4_2:12706786-12706805 None:intergenic 35.0%
!! GCTATTAGGTTTTATCCCTT+TGG - chr4_2:12706789-12706808 Msa0589600:five_prime_UTR 35.0%
ATTCACACATTAGAGAAGGC+TGG + chr4_2:12700532-12700551 None:intergenic 40.0%
GTTTGGTTATGCTCACATGA+AGG + chr4_2:12700564-12700583 None:intergenic 40.0%
! TAGAGGTGGTGGTTAAATTG+GGG + chr4_2:12700680-12700699 None:intergenic 40.0%
! GTTAGAGGTGGTGGTTAAAT+TGG + chr4_2:12700682-12700701 None:intergenic 40.0%
!! TTCGTTGTTCGGTTTGGTTT+GGG + chr4_2:12700805-12700824 None:intergenic 40.0%
!! ATTCGTTGTTCGGTTTGGTT+TGG + chr4_2:12700806-12700825 None:intergenic 40.0%
GAGAAACAATGCAGCATCTT+TGG + chr4_2:12700886-12700905 None:intergenic 40.0%
TCGAATCAAACTTCTCATGC+AGG - chr4_2:12700956-12700975 Msa0589600:intron 40.0%
!!! TGTAGTTTTTTGCAGACTCG+TGG - chr4_2:12701049-12701068 Msa0589600:intron 40.0%
! GTGTAACTCTTTTACACCGA+CGG + chr4_2:12701155-12701174 None:intergenic 40.0%
!!! GTGTAGTTTTTTCTCCTTCC+GGG + chr4_2:12701454-12701473 None:intergenic 40.0%
!!! GGTGTAGTTTTTTCTCCTTC+CGG + chr4_2:12701455-12701474 None:intergenic 40.0%
AACTACACCGAGATTAAACG+AGG - chr4_2:12701466-12701485 Msa0589600:intron 40.0%
ACTACACCGAGATTAAACGA+GGG - chr4_2:12701467-12701486 Msa0589600:intron 40.0%
TTCTGTCCCTCGTTTAATCT+CGG + chr4_2:12701476-12701495 None:intergenic 40.0%
TGGTTATAGCTGATGCTTTG+GGG + chr4_2:12701501-12701520 None:intergenic 40.0%
TCCGACTCAGTTTAGTCATT+TGG + chr4_2:12701521-12701540 None:intergenic 40.0%
!! GCAGATGCTTTAGCTGATTT+TGG + chr4_2:12701583-12701602 None:intergenic 40.0%
!! TCTTCTTCAGATGGATTGTG+AGG + chr4_2:12701644-12701663 None:intergenic 40.0%
! GGAGATTGATTCTTCTTTGG+TGG + chr4_2:12701734-12701753 None:intergenic 40.0%
! GGTGGAGATTGATTCTTCTT+TGG + chr4_2:12701737-12701756 None:intergenic 40.0%
! AGTTATGGGGAGTGTATGAA+GGG + chr4_2:12701798-12701817 None:intergenic 40.0%
! GAAGGTTTTGCGAAGAGAAT+TGG + chr4_2:12701844-12701863 None:intergenic 40.0%
AGAGCTATTTGAGGAACTCT+TGG + chr4_2:12702350-12702369 None:intergenic 40.0%
AAGAGTTCCTCAAATAGCTC+TGG - chr4_2:12702349-12702368 Msa0589600:CDS 40.0%
ACAAGTTCCAGAGCTATTTG+AGG + chr4_2:12702359-12702378 None:intergenic 40.0%
AATGCAAGTTGTGCATGAGA+TGG + chr4_2:12702930-12702949 None:intergenic 40.0%
ATACCCTTTAGACTGCAAAC+TGG + chr4_2:12703022-12703041 None:intergenic 40.0%
GTAATTAACTCCCCTGATCA+AGG - chr4_2:12703079-12703098 Msa0589600:CDS 40.0%
CTTACGTTGACAAGATTTGC+AGG - chr4_2:12703240-12703259 Msa0589600:intron 40.0%
TTGCAGGCCATATCAATGAT+AGG - chr4_2:12703256-12703275 Msa0589600:intron 40.0%
TGCAGGCCATATCAATGATA+GGG - chr4_2:12703257-12703276 Msa0589600:intron 40.0%
TCAATGATAGGGAAGGAAGA+AGG - chr4_2:12703268-12703287 Msa0589600:CDS 40.0%
AGGCATTAAAGGTTACTGGA+AGG - chr4_2:12703288-12703307 Msa0589600:CDS 40.0%
GGCATTAAAGGTTACTGGAA+GGG - chr4_2:12703289-12703308 Msa0589600:CDS 40.0%
AAGAGCTGAACAGCACTATA+AGG + chr4_2:12703427-12703446 None:intergenic 40.0%
GTACCAAACATTGCATGTTC+AGG - chr4_2:12703498-12703517 Msa0589600:intron 40.0%
ACACCTGAACATGCAATGTT+TGG + chr4_2:12703504-12703523 None:intergenic 40.0%
CGTAATCTCAGGACATCTAA+AGG + chr4_2:12703837-12703856 None:intergenic 40.0%
CAACAGCTAATCGTAATCTC+AGG + chr4_2:12703848-12703867 None:intergenic 40.0%
!! AGATGCCATTTCAGGTTAGT+TGG - chr4_2:12704332-12704351 Msa0589600:intron 40.0%
CAATGCCAACTAACCTGAAA+TGG + chr4_2:12704340-12704359 None:intergenic 40.0%
! CGCAAGTTTTGCACAGATAA+AGG + chr4_2:12704439-12704458 None:intergenic 40.0%
!! ACTTTGAAGCAGCTTGTGAT+TGG - chr4_2:12704909-12704928 Msa0589600:intron 40.0%
GGAGTTTCAAACAATTGCAG+AGG - chr4_2:12705005-12705024 Msa0589600:intron 40.0%
GGAAATGGCCTATATTACAG+GGG + chr4_2:12705118-12705137 None:intergenic 40.0%
CGGAAATGGCCTATATTACA+GGG + chr4_2:12705119-12705138 None:intergenic 40.0%
CCTGTAATATAGGCCATTTC+CGG - chr4_2:12705117-12705136 Msa0589600:intron 40.0%
ATTAAGCACTTCCTTAGAGG+AGG - chr4_2:12705908-12705927 Msa0589600:intron 40.0%
CCGGAAATGACCTATATTAC+AGG - chr4_2:12706500-12706519 Msa0589600:CDS 40.0%
TAGCAGTGAGATATCTCAGA+AGG - chr4_2:12706603-12706622 Msa0589600:CDS 40.0%
AGCAGTGAGATATCTCAGAA+GGG - chr4_2:12706604-12706623 Msa0589600:CDS 40.0%
! GTAGGACTCAAGTTTGTTCT+AGG - chr4_2:12706640-12706659 Msa0589600:CDS 40.0%
GTATGAACAGACTTCTCCTA+TGG - chr4_2:12706767-12706786 Msa0589600:five_prime_UTR 40.0%
AGACTTCTCCTATGGCTATT+AGG - chr4_2:12706775-12706794 Msa0589600:five_prime_UTR 40.0%
TTCGTTCTACTGCGTGTGTT+TGG + chr4_2:12700581-12700600 None:intergenic 45.0%
GAGTTGTTGAAGAGGTGGTT+GGG + chr4_2:12700717-12700736 None:intergenic 45.0%
TGAAGGTGGAGTTGTTGAAG+AGG + chr4_2:12700725-12700744 None:intergenic 45.0%
GAATTTGGAAGGAATGGTGG+TGG + chr4_2:12700766-12700785 None:intergenic 45.0%
ACAAAGAGGGCTAAAAAGCC+CGG - chr4_2:12701433-12701452 Msa0589600:intron 45.0%
GGTTATAGCTGATGCTTTGG+GGG + chr4_2:12701500-12701519 None:intergenic 45.0%
!! TCTTCAGATGGATTGTGAGG+TGG + chr4_2:12701641-12701660 None:intergenic 45.0%
GATTCGTCGTCTTCTTCAGA+TGG + chr4_2:12701653-12701672 None:intergenic 45.0%
GGGATTTCAAGCTATAGAGG+TGG + chr4_2:12701755-12701774 None:intergenic 45.0%
GCTGGGATTTCAAGCTATAG+AGG + chr4_2:12701758-12701777 None:intergenic 45.0%
TTGAGGTATGTCAGAAGGCT+GGG + chr4_2:12701775-12701794 None:intergenic 45.0%
AAGGGTTGAGGTATGTCAGA+AGG + chr4_2:12701780-12701799 None:intergenic 45.0%
! GAGTTATGGGGAGTGTATGA+AGG + chr4_2:12701799-12701818 None:intergenic 45.0%
ACTCAGGAATGCTCTAGCAT+AGG + chr4_2:12702442-12702461 None:intergenic 45.0%
CTGCCGATGGAATTGTGAAT+AGG - chr4_2:12702509-12702528 Msa0589600:intron 45.0%
GGGCCTATTCACAATTCCAT+CGG + chr4_2:12702515-12702534 None:intergenic 45.0%
!! ATAGATGTGGCTCTAGAAGG+AGG - chr4_2:12702793-12702812 Msa0589600:intron 45.0%
!! TAGATGTGGCTCTAGAAGGA+GGG - chr4_2:12702794-12702813 Msa0589600:intron 45.0%
TCTAGAAGGAGGGGAACATT+TGG - chr4_2:12702804-12702823 Msa0589600:intron 45.0%
CTATATGTACGACTTGCCTC+TGG - chr4_2:12702964-12702983 Msa0589600:intron 45.0%
TATTGTCAAGGAGTTTGGGG+AGG + chr4_2:12703148-12703167 None:intergenic 45.0%
GGCCATATCAATGATAGGGA+AGG - chr4_2:12703261-12703280 Msa0589600:CDS 45.0%
!! GGGAAGGAAGAAGGCATTAA+AGG - chr4_2:12703277-12703296 Msa0589600:CDS 45.0%
TATCTAGTGATGCTCACCTG+TGG + chr4_2:12703322-12703341 None:intergenic 45.0%
AACGATCAGCTCTCTGTTGT+CGG - chr4_2:12703565-12703584 Msa0589600:intron 45.0%
TTTATAGGTCGCCTTGAGCA+TGG - chr4_2:12703961-12703980 Msa0589600:intron 45.0%
TTCCTCTCTTACCATGCTCA+AGG + chr4_2:12703975-12703994 None:intergenic 45.0%
TTGAGCATGGTAAGAGAGGA+AGG - chr4_2:12703974-12703993 Msa0589600:intron 45.0%
TTCCGATAAGAGAAGGACCA+AGG + chr4_2:12704021-12704040 None:intergenic 45.0%
GGAGCTATTCCGATAAGAGA+AGG + chr4_2:12704028-12704047 None:intergenic 45.0%
CAGAGGATAGCATGTAAGTG+TGG + chr4_2:12704259-12704278 None:intergenic 45.0%
CACACTTACATGCTATCCTC+TGG - chr4_2:12704257-12704276 Msa0589600:intron 45.0%
GTGGCACAAGATGGAGTTAT+TGG - chr4_2:12704597-12704616 Msa0589600:intron 45.0%
!! AAGCATTGGGCACAAATCCT+CGG + chr4_2:12704629-12704648 None:intergenic 45.0%
! ACAAGTACCTGCTGTTTGGT+AGG + chr4_2:12704659-12704678 None:intergenic 45.0%
GATCACAAGTACCTGCTGTT+TGG + chr4_2:12704663-12704682 None:intergenic 45.0%
CCTGATGCTGCAAAAACACA+AGG + chr4_2:12704938-12704957 None:intergenic 45.0%
!! CCTTGTGTTTTTGCAGCATC+AGG - chr4_2:12704935-12704954 Msa0589600:intron 45.0%
CCGGAAATGGCCTATATTAC+AGG + chr4_2:12705120-12705139 None:intergenic 45.0%
CATAAGCGAATCTGCTGAGT+TGG + chr4_2:12705222-12705241 None:intergenic 45.0%
ACTCAGCAGATTCGCTTATG+TGG - chr4_2:12705222-12705241 Msa0589600:intron 45.0%
GCAGTGAGATATCTCAGAAG+GGG - chr4_2:12706605-12706624 Msa0589600:CDS 45.0%
!!! TAAATGAATTTAAATTTTTT+TGG + chr4_2:12705337-12705356 None:intergenic 5.0%
!!! TTTTTAATTTTAAAAAAATC+TGG + chr4_2:12705714-12705733 None:intergenic 5.0%
!!! TCATTTTTTATTTTTTTAAA+TGG + chr4_2:12706358-12706377 None:intergenic 5.0%
ATTGGGGATATTGTGCCACG+TGG + chr4_2:12700664-12700683 None:intergenic 50.0%
TGAAACGACGTCGTTAGAGG+TGG + chr4_2:12700694-12700713 None:intergenic 50.0%
GGAGTTGTTGAAGAGGTGGT+TGG + chr4_2:12700718-12700737 None:intergenic 50.0%
AGGTGGAGTTGTTGAAGAGG+TGG + chr4_2:12700722-12700741 None:intergenic 50.0%
!! TTTGGAAGGAATGGTGGTGG+TGG + chr4_2:12700763-12700782 None:intergenic 50.0%
GGCGAATTTGGAAGGAATGG+TGG + chr4_2:12700769-12700788 None:intergenic 50.0%
GGTGGCGAATTTGGAAGGAA+TGG + chr4_2:12700772-12700791 None:intergenic 50.0%
!! TTTGGTTTGGGAGGTGGAGA+CGG + chr4_2:12700793-12700812 None:intergenic 50.0%
!! GTTGTTCGGTTTGGTTTGGG+AGG + chr4_2:12700802-12700821 None:intergenic 50.0%
CGCGAATTCGTTGTTCGGTT+TGG + chr4_2:12700811-12700830 None:intergenic 50.0%
TTAAGAAGCTGAGCAGGCGT+CGG + chr4_2:12700834-12700853 None:intergenic 50.0%
TTTGGGCACAAAAGCGACGA+CGG + chr4_2:12700868-12700887 None:intergenic 50.0%
! GATGCTGCATTGTTTCTCGC+CGG - chr4_2:12700888-12700907 Msa0589600:intron 50.0%
TCGATCAAGTGGAGCAGTGA+CGG + chr4_2:12700940-12700959 None:intergenic 50.0%
!! TCAGATGGATTGTGAGGTGG+TGG + chr4_2:12701638-12701657 None:intergenic 50.0%
GTTGAGGTATGTCAGAAGGC+TGG + chr4_2:12701776-12701795 None:intergenic 50.0%
TCCCCATAACTCTGCCATGT+AGG - chr4_2:12701807-12701826 Msa0589600:intron 50.0%
GCCTACATGGCAGAGTTATG+GGG + chr4_2:12701811-12701830 None:intergenic 50.0%
CGCCTACATGGCAGAGTTAT+GGG + chr4_2:12701812-12701831 None:intergenic 50.0%
AGTAGCTGCAGAGCTTGCAA+AGG + chr4_2:12702412-12702431 None:intergenic 50.0%
CAAGCTCTGCAGCTACTGAT+AGG - chr4_2:12702415-12702434 Msa0589600:intron 50.0%
!! AGATGTGGCTCTAGAAGGAG+GGG - chr4_2:12702795-12702814 Msa0589600:intron 50.0%
TAGTGATGCTCACCTGTGGT+AGG + chr4_2:12703318-12703337 None:intergenic 50.0%
!! TGGGTGTCGAACAATGACAG+AGG - chr4_2:12703872-12703891 Msa0589600:intron 50.0%
GCTTCTTTCTACTACGGCCT+TGG - chr4_2:12704001-12704020 Msa0589600:intron 50.0%
GGCCTTGGTCCTTCTCTTAT+CGG - chr4_2:12704016-12704035 Msa0589600:CDS 50.0%
! AAGAGATGCTGAAAGCACGG+CGG + chr4_2:12704235-12704254 None:intergenic 50.0%
GGCAAGAGATGCTGAAAGCA+CGG + chr4_2:12704238-12704257 None:intergenic 50.0%
TCGCAGACAGATGCAACTGA+AGG - chr4_2:12704287-12704306 Msa0589600:intron 50.0%
CGCAGACAGATGCAACTGAA+GGG - chr4_2:12704288-12704307 Msa0589600:intron 50.0%
! GCAGGTATTGTGGCACAAGA+TGG - chr4_2:12704588-12704607 Msa0589600:intron 50.0%
!! GGAGTTATTGGCTTGTACCG+AGG - chr4_2:12704609-12704628 Msa0589600:intron 50.0%
CCTTCTCACTAGCAGCAATC+AGG + chr4_2:12704987-12705006 None:intergenic 50.0%
CCTGATTGCTGCTAGTGAGA+AGG - chr4_2:12704984-12705003 Msa0589600:intron 50.0%
ACAAGGGCGAAAACCGGAAA+TGG + chr4_2:12706487-12706506 None:intergenic 50.0%
! AGCGAATCTGCTGAGTTGGA+GGG + chr4_2:12706405-12706424 None:intergenic 50.0%
! AAGCGAATCTGCTGAGTTGG+AGG + chr4_2:12706404-12706423 None:intergenic 50.0%
ACTCAGCAGATTCGCTTACG+TGG + chr4_2:12706401-12706420 None:intergenic 50.0%
CGTAAGCGAATCTGCTGAGT+TGG - chr4_2:12706401-12706420 Msa0589600:CDS 50.0%
! AAGCGAATCTGCTGAGTTGG+AGG - chr4_2:12706404-12706423 Msa0589600:CDS 50.0%
! AGCGAATCTGCTGAGTTGGA+GGG - chr4_2:12706405-12706424 Msa0589600:CDS 50.0%
GATATTGTGCCACGTGGCGA+CGG + chr4_2:12700658-12700677 None:intergenic 55.0%
AACGACGTCGTTAGAGGTGG+TGG + chr4_2:12700691-12700710 None:intergenic 55.0%
GGGTGAAACGACGTCGTTAG+AGG + chr4_2:12700697-12700716 None:intergenic 55.0%
! TGCGTCGGTGAAAGTGAAGG+TGG + chr4_2:12700739-12700758 None:intergenic 55.0%
! GGTTGCGTCGGTGAAAGTGA+AGG + chr4_2:12700742-12700761 None:intergenic 55.0%
!! AATGGTGGTGGTGGTTGCGT+CGG + chr4_2:12700754-12700773 None:intergenic 55.0%
GAGACGGTGGCGAATTTGGA+AGG + chr4_2:12700777-12700796 None:intergenic 55.0%
!! GTTCGGTTTGGTTTGGGAGG+TGG + chr4_2:12700799-12700818 None:intergenic 55.0%
GTCGGCGCGAATTCGTTGTT+CGG + chr4_2:12700816-12700835 None:intergenic 55.0%
GGGTGGTTAAGAAGCTGAGC+AGG + chr4_2:12700840-12700859 None:intergenic 55.0%
ACAAAAGCGACGACGGCGAT+CGG + chr4_2:12700861-12700880 None:intergenic 55.0%
!! GACGGTTTTAGCAGCAGCTC+CGG + chr4_2:12700922-12700941 None:intergenic 55.0%
CAGACTCGTGGAGTGCGAAT+TGG - chr4_2:12701061-12701080 Msa0589600:intron 55.0%
AGAGGGCTAAAAAGCCCGGA+AGG - chr4_2:12701437-12701456 Msa0589600:intron 55.0%
! GGGGAGTGTATGAAGGGTTG+AGG + chr4_2:12701792-12701811 None:intergenic 55.0%
GCGCCTACATGGCAGAGTTA+TGG + chr4_2:12701813-12701832 None:intergenic 55.0%
! TGGGAAGTGTAGCGCCTACA+TGG + chr4_2:12701824-12701843 None:intergenic 55.0%
TTCAGAGCATGCTCTGCCGA+TGG - chr4_2:12702496-12702515 Msa0589600:intron 55.0%
CTGGAAGGGTAACCTACCAC+AGG - chr4_2:12703303-12703322 Msa0589600:CDS 55.0%
GTTGTCGGAAGACTTGCAGC+TGG - chr4_2:12703580-12703599 Msa0589600:intron 55.0%
! CTTGCAGCTGGTGCTTTTGC+TGG - chr4_2:12703592-12703611 Msa0589600:intron 55.0%
CGCCTTGAGCATGGTAAGAG+AGG - chr4_2:12703970-12703989 Msa0589600:intron 55.0%
TGTCTGCGAACTGTGTCCAG+AGG + chr4_2:12704276-12704295 None:intergenic 55.0%
! GAATCTGCTGAGTTGGAGGG+GGG + chr4_2:12706408-12706427 None:intergenic 55.0%
! CGAATCTGCTGAGTTGGAGG+GGG + chr4_2:12706407-12706426 None:intergenic 55.0%
! GCGAATCTGCTGAGTTGGAG+GGG + chr4_2:12706406-12706425 None:intergenic 55.0%
! TCGCTTATGTGGCGCTGAGT+TGG - chr4_2:12705233-12705252 Msa0589600:intron 55.0%
! GCGAATCTGCTGAGTTGGAG+GGG - chr4_2:12706406-12706425 Msa0589600:CDS 55.0%
! CGAATCTGCTGAGTTGGAGG+GGG - chr4_2:12706407-12706426 Msa0589600:CDS 55.0%
! GAATCTGCTGAGTTGGAGGG+GGG - chr4_2:12706408-12706427 Msa0589600:CDS 55.0%
CGAACAAAACCGTCGCCACG+TGG - chr4_2:12700646-12700665 Msa0589600:three_prime_UTR 60.0%
GGTGGAGACGGTGGCGAATT+TGG + chr4_2:12700781-12700800 None:intergenic 60.0%
CAAAAGCGACGACGGCGATC+GGG + chr4_2:12700860-12700879 None:intergenic 60.0%
!! TTTCTCGCCGGTGCTGTTGC+CGG - chr4_2:12700900-12700919 Msa0589600:intron 60.0%
! TCGCTTACGTGGCGCTGAGT+TGG + chr4_2:12706390-12706409 None:intergenic 60.0%
GGTTTGGGAGGTGGAGACGG+TGG + chr4_2:12700790-12700809 None:intergenic 65.0%
AGCAGCTCCGGCAACAGCAC+CGG + chr4_2:12700910-12700929 None:intergenic 65.0%
!!! GAGGGGGGAGGGGTGTTTTC+CGG + chr4_2:12706423-12706442 None:intergenic 65.0%
! TGCTGAGTTGGAGGGGGGAG+GGG + chr4_2:12706413-12706432 None:intergenic 65.0%
! CTGCTGAGTTGGAGGGGGGA+GGG + chr4_2:12706412-12706431 None:intergenic 65.0%
! TCTGCTGAGTTGGAGGGGGG+AGG + chr4_2:12706411-12706430 None:intergenic 65.0%
! TCTGCTGAGTTGGAGGGGGG+AGG - chr4_2:12706411-12706430 Msa0589600:CDS 65.0%
! CTGCTGAGTTGGAGGGGGGA+GGG - chr4_2:12706412-12706431 Msa0589600:CDS 65.0%
! TGCTGAGTTGGAGGGGGGAG+GGG - chr4_2:12706413-12706432 Msa0589600:CDS 65.0%
!!! GAGGGGGGAGGGGTGTTTTC+CGG - chr4_2:12706423-12706442 Msa0589600:CDS 65.0%
AAGCGACGACGGCGATCGGG+TGG + chr4_2:12700857-12700876 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 12700525 12706815 12700525 ID=Msa0589600;Name=Msa0589600
chr4_2 mRNA 12700525 12706815 12700525 ID=Msa0589600-mRNA-1;Parent=Msa0589600;Name=Msa0589600-mRNA-1;_AED=0.19;_eAED=0.19;_QI=108|1|1|1|0.9|0.81|11|299|381
chr4_2 exon 12700525 12700792 12700525 ID=Msa0589600-mRNA-1:exon:3698;Parent=Msa0589600-mRNA-1
chr4_2 exon 12702250 12702389 12702250 ID=Msa0589600-mRNA-1:exon:3697;Parent=Msa0589600-mRNA-1
chr4_2 exon 12702670 12702748 12702670 ID=Msa0589600-mRNA-1:exon:3696;Parent=Msa0589600-mRNA-1
chr4_2 exon 12702995 12703164 12702995 ID=Msa0589600-mRNA-1:exon:3695;Parent=Msa0589600-mRNA-1
chr4_2 exon 12703260 12703372 12703260 ID=Msa0589600-mRNA-1:exon:3694;Parent=Msa0589600-mRNA-1
chr4_2 exon 12703447 12703515 12703447 ID=Msa0589600-mRNA-1:exon:3693;Parent=Msa0589600-mRNA-1
chr4_2 exon 12703710 12703793 12703710 ID=Msa0589600-mRNA-1:exon:3692;Parent=Msa0589600-mRNA-1
chr4_2 exon 12703875 12703931 12703875 ID=Msa0589600-mRNA-1:exon:3691;Parent=Msa0589600-mRNA-1
chr4_2 exon 12704016 12704078 12704016 ID=Msa0589600-mRNA-1:exon:3690;Parent=Msa0589600-mRNA-1
chr4_2 exon 12706220 12706276 12706220 ID=Msa0589600-mRNA-1:exon:3689;Parent=Msa0589600-mRNA-1
chr4_2 exon 12706363 12706815 12706363 ID=Msa0589600-mRNA-1:exon:3688;Parent=Msa0589600-mRNA-1
chr4_2 five_prime_UTR 12706708 12706815 12706708 ID=Msa0589600-mRNA-1:five_prime_utr;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12706363 12706707 12706363 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12706220 12706276 12706220 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12704016 12704078 12704016 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12703875 12703931 12703875 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12703710 12703793 12703710 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12703447 12703515 12703447 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12703260 12703372 12703260 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12702995 12703164 12702995 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12702670 12702748 12702670 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 CDS 12702281 12702389 12702281 ID=Msa0589600-mRNA-1:cds;Parent=Msa0589600-mRNA-1
chr4_2 three_prime_UTR 12702250 12702280 12702250 ID=Msa0589600-mRNA-1:three_prime_utr;Parent=Msa0589600-mRNA-1
chr4_2 three_prime_UTR 12700525 12700792 12700525 ID=Msa0589600-mRNA-1:three_prime_utr;Parent=Msa0589600-mRNA-1
Gene Sequence

>Msa0589600

ATGACAAACTCAACGAACAAAACCGTCGCCACGTGGCACAATATCCCCAATTTAACCACCACCTCTAACGACGTCGTTTCACCCAACCACCTCTTCAACAACTCCACCTTCACTTTCACCGACGCAACCACCACCACCATTCCTTCCAAATTCGCCACCGTCTCCACCTCCCAAACCAAACCGAACAACGAATTCGCGCCGACGCCTGCTCAGCTTCTTAACCACCCGATCGCCGTCGTCGCTTTTGTGCCCAAAGATGCTGCATTGTTTCTCGCCGGTGCTGTTGCCGGAGCTGCTGCTAAAACCGTCACTGCTCCACTTGATCGAATCAAACTTCTCATGCAGACTCGTGGAGTGCGAATTGGACAAGAAAGTGCTAAGAAGACAATTGGTTTCATTGAGGCCATATCAATGATAGGGAAGGAAGAAGGCATTAAAGGTTACTGGAAGGGTAACCTACCACAGGTGATTAGGGTTATACCTTATAGTGCTGTTCAGCTCTTTGCTTATGAAATTTATAAGAAAATATTCAAGGGAAAAAACGATCAGCTCTCTGTTGTCGGAAGACTTGCAGCTGGTGCTTTTGCTGGCATGACATCAACTTTTATAACTTACCCTTTAGATGTCCTGAGATTACGATTAGCTGTTGAACTTGGGTGTCGAACAATGACAGAGGTCGCCTTGAGCATGGTAAGAGAGGAAGGTTTTGCTTCTTTCTACTACGGCCTTGGTCCTTCTCTTATCGGAATAGCTCCATATATTGCAGTTAACTTTTGTGTTTTTGACTTATTAAAGAAGTCGTTGCCAGAGAAATATCAAAAGAGAACTGAAACATCTCTAGTCACCGCCGTGCTTTCAGCATCTCTTGCCACACTTACATGCTATCCTCTGGACACAGTTCGCAGACAGATGCAACTGAAGGGTACACCTTATAAGACAGTATTAGATGCCATTTCAGGTATTGTGGCACAAGATGGAGTTATTGGCTTGTACCGAGGATTTGTGCCCAATGCTTTAAAAAACCTACCAAACAGCAGCATCAGGCTTACAACATATGACATTGTCAAACGCCTGATTGCTGCTAGTGAGAAGGAGTTTCAAACAATTGCAGAGGAAAACCGCAACAAACTAAAGAAATGCCCATAA
Protein sequence

>Msa0589600

MTNSTNKTVATWHNIPNLTTTSNDVVSPNHLFNNSTFTFTDATTTTIPSKFATVSTSQTKPNNEFAPTPAQLLNHPIAVVAFVPKDAALFLAGAVAGAAAKTVTAPLDRIKLLMQTRGVRIGQESAKKTIGFIEAISMIGKEEGIKGYWKGNLPQVIRVIPYSAVQLFAYEIYKKIFKGKNDQLSVVGRLAAGAFAGMTSTFITYPLDVLRLRLAVELGCRTMTEVALSMVREEGFASFYYGLGPSLIGIAPYIAVNFCVFDLLKKSLPEKYQKRTETSLVTAVLSASLATLTCYPLDTVRRQMQLKGTPYKTVLDAISGIVAQDGVIGLYRGFVPNALKNLPNSSIRLTTYDIVKRLIAASEKEFQTIAEENRNKLKKCP*