AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0516330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0516330 MtrunA17_Chr3g0126901 97.727 440 10 0 1 440 1 440 0.0 897
Msa0516330 MtrunA17_Chr1g0146931 57.078 438 144 12 40 436 49 483 4.44e-106 323
Msa0516330 MtrunA17_Chr8g0346021 32.857 420 195 16 1 363 1 390 2.44e-36 140
Msa0516330 MtrunA17_Chr6g0462191 39.236 288 139 8 1 272 1 268 1.00e-28 117
Msa0516330 MtrunA17_Chr7g0273861 28.758 459 224 19 1 404 1 411 1.24e-28 117
Msa0516330 MtrunA17_Chr1g0200161 37.640 178 85 4 112 275 141 306 9.46e-27 112
Msa0516330 MtrunA17_Chr4g0020671 25.575 391 234 12 1 368 1 357 6.20e-26 109
Msa0516330 MtrunA17_Chr1g0181581 26.591 440 241 18 1 372 1 426 6.82e-26 109
Msa0516330 MtrunA17_Chr7g0263731 35.517 290 146 10 1 272 1 267 2.05e-25 108
Msa0516330 MtrunA17_Chr4g0025441 38.660 194 77 6 107 281 88 258 2.33e-24 104
Msa0516330 MtrunA17_Chr4g0045641 26.609 466 236 15 1 372 1 454 1.91e-23 102
Msa0516330 MtrunA17_Chr1g0204901 58.779 131 48 3 56 180 74 204 4.29e-22 98.2
Msa0516330 MtrunA17_Chr1g0156121 34.884 172 68 5 112 273 124 261 7.21e-21 95.5
Msa0516330 MtrunA17_Chr8g0371131 60.526 76 29 1 109 184 68 142 8.49e-21 88.2
Msa0516330 MtrunA17_Chr6g0472551 34.343 198 94 5 114 311 119 280 3.02e-20 92.8
Msa0516330 MtrunA17_Chr1g0203711 63.768 69 25 0 112 180 115 183 1.60e-19 90.5
Msa0516330 MtrunA17_Chr3g0135851 42.857 168 88 5 112 272 91 257 1.67e-19 90.5
Msa0516330 MtrunA17_Chr1g0172611 28.571 399 219 11 4 376 3 361 1.91e-19 89.7
Msa0516330 MtrunA17_Chr8g0389781 58.442 77 27 1 101 172 110 186 4.92e-19 89.0
Msa0516330 MtrunA17_Chr8g0340761 43.137 204 98 5 1 188 1 202 7.84e-19 88.2
Msa0516330 MtrunA17_Chr7g0273951 58.571 70 29 0 111 180 105 174 2.38e-18 86.3
Msa0516330 MtrunA17_Chr4g0060381 35.862 145 72 3 110 247 98 228 2.66e-18 87.4
Msa0516330 MtrunA17_Chr2g0330921 52.174 92 37 1 97 181 75 166 2.17e-17 83.2
Msa0516330 MtrunA17_Chr1g0166431 56.579 76 32 1 90 164 94 169 6.40e-17 82.4
Msa0516330 MtrunA17_Chr2g0283321 30.939 181 105 6 114 286 65 233 4.02e-15 75.9
Msa0516330 MtrunA17_Chr4g0057321 58.209 67 27 1 114 179 90 156 2.19e-14 74.7
Msa0516330 MtrunA17_Chr2g0330051 35.664 143 76 6 141 272 107 244 2.12e-12 68.6
Msa0516330 MtrunA17_Chr5g0416161 32.386 176 72 7 110 274 117 256 9.81e-12 67.0
Msa0516330 MtrunA17_Chr2g0316401 26.257 179 106 5 114 280 76 240 1.16e-11 65.5
Msa0516330 MtrunA17_Chr7g0215371 52.632 57 27 0 112 168 19 75 1.86e-11 60.5
Msa0516330 MtrunA17_Chr8g0342551 59.036 83 32 1 110 190 88 170 3.39e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0516330 AT3G49260.1 47.333 450 190 17 1 408 1 445 1.09e-87 275
Msa0516330 AT3G49260.4 47.333 450 190 17 1 408 1 445 1.09e-87 275
Msa0516330 AT3G49260.2 47.333 450 190 17 1 408 1 445 1.09e-87 275
Msa0516330 AT3G49260.3 47.450 451 189 17 1 408 1 446 2.29e-87 274
Msa0516330 AT5G03040.1 30.189 424 218 13 1 363 1 407 3.72e-38 144
Msa0516330 AT5G03040.3 30.189 424 218 13 1 363 1 407 3.72e-38 144
Msa0516330 AT5G03040.2 30.189 424 218 13 1 363 1 407 3.72e-38 144
Msa0516330 AT3G52290.1 33.103 435 188 15 5 370 2 402 3.96e-37 140
Msa0516330 AT4G00820.1 32.239 335 179 11 63 373 85 395 3.68e-31 125
Msa0516330 AT1G01110.2 31.421 401 219 14 1 367 1 379 9.28e-31 124
Msa0516330 AT3G09710.1 31.104 299 142 10 4 272 3 267 8.51e-30 120
Msa0516330 AT3G09710.2 29.712 313 142 11 4 272 3 281 6.67e-28 115
Msa0516330 AT4G10640.1 28.358 402 217 14 1 367 1 366 5.06e-27 112
Msa0516330 AT2G26180.1 41.262 206 112 5 112 309 84 288 1.47e-26 111
Msa0516330 AT3G59690.2 31.317 281 148 10 115 367 172 435 1.27e-24 106
Msa0516330 AT3G59690.1 31.317 281 148 10 115 367 172 435 1.27e-24 106
Msa0516330 AT3G22190.3 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa0516330 AT3G22190.2 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa0516330 AT3G22190.4 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa0516330 AT3G22190.1 41.799 189 81 4 105 281 81 252 5.26e-23 100
Msa0516330 AT1G72670.1 29.178 377 221 12 1 357 1 351 1.33e-22 99.8
Msa0516330 AT5G13460.1 30.888 259 140 11 114 360 113 344 1.78e-22 99.8
Msa0516330 AT3G49380.1 39.896 193 84 6 1 178 1 176 2.76e-22 97.8
Msa0516330 AT4G23060.1 62.821 78 28 1 101 178 157 233 3.59e-21 96.3
Msa0516330 AT4G14750.1 66.667 66 22 0 112 177 105 170 7.65e-21 94.4
Msa0516330 AT4G14750.3 66.667 66 22 0 112 177 164 229 1.23e-20 94.4
Msa0516330 AT4G14750.2 66.667 66 22 0 112 177 164 229 1.24e-20 94.4
Msa0516330 AT1G17480.1 42.063 126 67 2 110 235 92 211 3.27e-20 92.0
Msa0516330 AT2G43680.4 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa0516330 AT2G43680.2 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa0516330 AT2G43680.3 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa0516330 AT2G43680.5 28.014 282 160 9 114 368 324 589 1.13e-19 92.0
Msa0516330 AT2G43680.1 28.014 282 160 9 114 368 325 590 1.19e-19 92.0
Msa0516330 AT3G16490.1 50.000 96 43 1 101 196 96 186 1.02e-18 88.2
Msa0516330 AT2G26410.2 34.254 181 92 6 109 275 145 312 1.27e-18 88.6
Msa0516330 AT2G26410.1 34.254 181 92 6 109 275 145 312 1.27e-18 88.6
Msa0516330 AT5G07240.1 57.647 85 23 1 101 172 82 166 2.42e-18 87.0
Msa0516330 AT5G07240.2 57.647 85 23 1 101 172 128 212 3.01e-18 87.0
Msa0516330 AT1G18840.1 55.385 65 29 0 112 176 109 173 8.14e-18 86.3
Msa0516330 AT1G18840.3 55.385 65 29 0 112 176 109 173 8.14e-18 86.3
Msa0516330 AT1G18840.2 55.385 65 29 0 112 176 109 173 8.14e-18 86.3
Msa0516330 AT1G19870.1 33.929 168 62 5 112 272 215 340 1.06e-17 86.3
Msa0516330 AT1G19870.2 33.929 168 62 5 112 272 32 157 1.09e-17 85.9
Msa0516330 AT3G51380.1 56.338 71 30 1 109 179 34 103 1.15e-17 78.6
Msa0516330 AT4G29150.1 56.522 69 30 0 106 174 125 193 6.78e-16 79.7
Msa0516330 AT1G51960.1 42.308 104 56 2 114 216 102 202 1.80e-15 77.8
Msa0516330 AT5G62070.1 71.698 53 15 0 114 166 119 171 8.69e-15 76.3
Msa0516330 AT1G74690.1 53.846 65 30 0 112 176 113 177 1.40e-14 76.3
Msa0516330 AT1G14380.1 50.000 64 32 0 111 174 93 156 2.46e-14 75.9
Msa0516330 AT1G14380.3 50.000 64 32 0 111 174 93 156 2.46e-14 75.9
Msa0516330 AT1G14380.4 50.000 64 32 0 111 174 93 156 2.46e-14 75.9
Msa0516330 AT1G14380.6 50.000 64 32 0 111 174 93 156 2.68e-14 75.5
Msa0516330 AT1G14380.5 50.000 64 32 0 111 174 93 156 2.68e-14 75.5
Msa0516330 AT1G14380.2 50.000 64 32 0 111 174 93 156 2.68e-14 75.5
Msa0516330 AT1G14380.7 50.000 64 32 0 111 174 93 156 3.49e-14 75.1
Msa0516330 AT2G33990.1 29.208 202 127 8 114 309 49 240 7.47e-14 72.0
Msa0516330 AT5G03960.3 34.568 243 134 7 4 231 3 235 5.64e-12 67.8
Msa0516330 AT5G03960.2 34.568 243 134 7 4 231 3 235 5.64e-12 67.8
Msa0516330 AT5G03960.1 39.037 187 99 4 60 231 48 234 1.53e-11 66.2
Msa0516330 AT3G15050.1 28.090 178 110 4 111 280 49 216 1.67e-11 64.7

Find 145 sgRNAs with CRISPR-Local

Find 280 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTTAATTATTACTATTTA+TGG 0.027919 3_4:+67825758 Msa0516330:three_prime_UTR
TCAAACAATGTTAGGCCTTT+AGG 0.179218 3_4:+67825183 Msa0516330:CDS
GGGCTTGGGCTTCTAGTAAT+TGG 0.200692 3_4:-67825555 None:intergenic
CCTTCAATCTAAACGGATTT+CGG 0.207622 3_4:+67825596 Msa0516330:CDS
CAAACAATGTTAGGCCTTTA+GGG 0.216561 3_4:+67825184 Msa0516330:CDS
AAATCAAGGACACTGATTTC+AGG 0.259041 3_4:+67824452 Msa0516330:CDS
GGGCGTCGTTTGGATCTTAC+TGG 0.267147 3_4:+67825457 Msa0516330:CDS
CTTCAATCTAAACGGATTTC+GGG 0.269149 3_4:+67825597 Msa0516330:CDS
TGGCCTTTGCAGACTGTGTT+GGG 0.282948 3_4:-67825404 None:intergenic
GACTTAAATCATGAAATTCT+TGG 0.286842 3_4:-67825326 None:intergenic
CATCTTCCATAACAACAATT+AGG 0.288073 3_4:+67821652 Msa0516330:five_prime_UTR
CTTCCTTCATTCGTGATATC+TGG 0.297331 3_4:-67823588 None:intergenic
CTTTCACTATCATCAACATT+TGG 0.297842 3_4:-67825093 None:intergenic
TGAAATCCTAATTGTTGTTA+TGG 0.298589 3_4:-67821658 None:intergenic
AGCAGCAGAGGAGGTATCTA+TGG 0.333458 3_4:+67823542 Msa0516330:CDS
ACGTGCACTGCGTGCTTTGA+AGG 0.337011 3_4:+67824210 Msa0516330:CDS
CATGGTATAGTATAATTATA+AGG 0.337361 3_4:-67825696 None:intergenic
ATATCATCAGTGGGTGTATA+TGG 0.341287 3_4:-67825228 None:intergenic
CGTGTTTGTCGTGAGTCAAT+TGG 0.349060 3_4:-67824351 None:intergenic
AACGACGCCCGATGCTTTGA+TGG 0.352089 3_4:-67825444 None:intergenic
AGCAGCAGCTGAAGCTGCAG+TGG 0.352700 3_4:+67823674 Msa0516330:CDS
AATGTTAGGCCTTTAGGGTT+GGG 0.372284 3_4:+67825189 Msa0516330:CDS
ACATGGAGCTACAATGAATA+AGG 0.377871 3_4:+67824477 Msa0516330:CDS
TTTCACTATCATCAACATTT+GGG 0.379669 3_4:-67825092 None:intergenic
TGGGCCTTGACTCTTAATCT+TGG 0.382714 3_4:-67825424 None:intergenic
CCAACATTCATCTCAGAAAC+AGG 0.383345 3_4:+67821772 Msa0516330:five_prime_UTR
TCAAAGCATCGGGCGTCGTT+TGG 0.387573 3_4:+67825447 Msa0516330:CDS
CCAACCAGTTCCAATCCAAT+TGG 0.388280 3_4:-67825140 None:intergenic
AGAAGGAAAAGGAAGAGAAT+TGG 0.395607 3_4:+67823511 Msa0516330:intron
AGCTGCTAGAGTTGTGAGAT+TGG 0.400903 3_4:+67823710 Msa0516330:CDS
CCGAAATCCGTTTAGATTGA+AGG 0.401125 3_4:-67825596 None:intergenic
AGATGAATGTTGGTAGTTAC+TGG 0.406597 3_4:-67821762 None:intergenic
TATCACAATCAAACAATGTT+AGG 0.407700 3_4:+67825175 Msa0516330:CDS
ACACAAATGGTTGGAACTAT+AGG 0.408666 3_4:+67824413 Msa0516330:CDS
GCAAGCATTGGTAAGGGTTC+AGG 0.410589 3_4:+67824306 Msa0516330:CDS
TGGAGGTGTACCTAGCTATA+TGG 0.419424 3_4:+67825380 Msa0516330:CDS
TTGGCCTTTGCAGACTGTGT+TGG 0.420117 3_4:-67825405 None:intergenic
TATGATTCATCTGGCTCTGG+TGG 0.422243 3_4:+67825516 Msa0516330:CDS
GGCTATGATTCATCTGGCTC+TGG 0.428311 3_4:+67825513 Msa0516330:CDS
TGAGCGTGAAAGAGCCCAAT+TGG 0.428420 3_4:+67825125 Msa0516330:CDS
AGCTGAAGCTGCAGTGGCTG+CGG 0.436330 3_4:+67823680 Msa0516330:CDS
TGCTGCTGTTGCTGCAGCAT+AGG 0.442444 3_4:-67823655 None:intergenic
CGTTGGTGGTGTTTAGAGAT+AGG 0.443029 3_4:-67825348 None:intergenic
GAGCCCAATTGGATTGGAAC+TGG 0.444353 3_4:+67825136 Msa0516330:CDS
CGGGTGCTGGTGTTGAGGAT+TGG 0.453226 3_4:+67825616 Msa0516330:CDS
GGGATGGGCTATGATTCATC+TGG 0.454170 3_4:+67825507 Msa0516330:CDS
TCCATACGCTTTCAACTGCC+AGG 0.454990 3_4:+67824510 Msa0516330:CDS
TGACAGTGACACAAAACATA+TGG 0.456294 3_4:-67825848 None:intergenic
CTTGTCCAGCCATGACCTCC+AGG 0.458475 3_4:-67825648 None:intergenic
TTTGTGTCTAACTTTGTAGT+AGG 0.461209 3_4:-67824397 None:intergenic
AGGTCATGGACATAACTCTA+AGG 0.464310 3_4:+67823734 Msa0516330:CDS
CAATGTTAGGCCTTTAGGGT+TGG 0.466914 3_4:+67825188 Msa0516330:CDS
ATGATTCATCTGGCTCTGGT+GGG 0.467215 3_4:+67825517 Msa0516330:CDS
ATTCATGGGACAGTAATGTT+TGG 0.472342 3_4:-67821705 None:intergenic
CTCATTCAGTCCTACTATAG+AGG 0.474856 3_4:+67823774 Msa0516330:CDS
ATGATTTCTCTCTTCAGTCA+AGG 0.475457 3_4:-67823625 None:intergenic
TCTAAACGGATTTCGGGTGC+TGG 0.479590 3_4:+67825603 Msa0516330:CDS
ATGTTAGGCCTTTAGGGTTG+GGG 0.482998 3_4:+67825190 Msa0516330:CDS
ACGGAGTCCTCCGACTGGGA+TGG 0.488447 3_4:+67825491 Msa0516330:CDS
GCTAGAGTTGTGAGATTGGC+AGG 0.489676 3_4:+67823714 Msa0516330:CDS
TGATTTCTCTCTTCAGTCAA+GGG 0.491440 3_4:-67823624 None:intergenic
TTTGCATGGATTGAGTCTCT+TGG 0.491563 3_4:-67825288 None:intergenic
TTGTGTGTGGTCTAAACCTT+GGG 0.492357 3_4:+67825721 Msa0516330:three_prime_UTR
TCCTCTCAGATGGGTAAGAA+AGG 0.494472 3_4:+67821976 Msa0516330:exon
GGATTCGCCTTCAATCTAAA+CGG 0.496890 3_4:+67825589 Msa0516330:CDS
GGCCTTTGCAGACTGTGTTG+GGG 0.500229 3_4:-67825403 None:intergenic
AGTTACTGGTCCTTCATGTA+TGG 0.501378 3_4:-67821748 None:intergenic
ACCTGGCAGTTGAAAGCGTA+TGG 0.501521 3_4:-67824511 None:intergenic
TCAGCACGGAGTCCTCCGAC+TGG 0.503212 3_4:+67825486 Msa0516330:CDS
ACTACAAAGTTAGACACAAA+TGG 0.507272 3_4:+67824400 Msa0516330:CDS
CAACCAGTTCCAATCCAATT+GGG 0.509130 3_4:-67825139 None:intergenic
CGGAGTCCTCCGACTGGGAT+GGG 0.510075 3_4:+67825492 Msa0516330:CDS
TCAAGGCCCATCAAAGCATC+GGG 0.510279 3_4:+67825437 Msa0516330:CDS
ATTGGAACTGGTTGGAGCGT+TGG 0.514467 3_4:+67825148 Msa0516330:CDS
CATCAGTGGGTGTATATGGT+GGG 0.517012 3_4:-67825224 None:intergenic
GTATATGGTGGGGTCTCAAG+TGG 0.518909 3_4:-67825213 None:intergenic
TCAAGTGGCCCCAACCCTAA+AGG 0.519519 3_4:-67825198 None:intergenic
GTTGTGAGATTGGCAGGTCA+TGG 0.520639 3_4:+67823720 Msa0516330:CDS
ACATAGCCTTTCCTCTCAGA+TGG 0.522560 3_4:+67821966 Msa0516330:exon
GATGCATGCAAGCATTGGTA+AGG 0.524234 3_4:+67824299 Msa0516330:CDS
CAAACACGAGCGGACAGTAG+AGG 0.524630 3_4:+67824366 Msa0516330:CDS
GTCAAGGCCCATCAAAGCAT+CGG 0.524983 3_4:+67825436 Msa0516330:CDS
TCACCAGATATCACGAATGA+AGG 0.525050 3_4:+67823585 Msa0516330:CDS
TCATGAGGCCCATGTTTGCA+TGG 0.531097 3_4:-67825302 None:intergenic
CGTGCACTGCGTGCTTTGAA+GGG 0.531337 3_4:+67824211 Msa0516330:CDS
CATGAAATTCTTGGTTCATG+AGG 0.532356 3_4:-67825317 None:intergenic
GTGAAAGAGCCCAATTGGAT+TGG 0.532613 3_4:+67825130 Msa0516330:CDS
TACTAGGTATCCTCTATAGT+AGG 0.537083 3_4:-67823784 None:intergenic
TTCTTCAGACATATCATCAG+TGG 0.544019 3_4:-67825238 None:intergenic
ATCAGTGGGTGTATATGGTG+GGG 0.545609 3_4:-67825223 None:intergenic
CTCCACTGTGGTGTCGTTGG+TGG 0.546689 3_4:-67825362 None:intergenic
GATTTCGGGTGCTGGTGTTG+AGG 0.548721 3_4:+67825611 Msa0516330:CDS
CACCTCCACTGTGGTGTCGT+TGG 0.549460 3_4:-67825365 None:intergenic
TGATTCATCTGGCTCTGGTG+GGG 0.551322 3_4:+67825518 Msa0516330:CDS
TTTAGACCACACACAATACA+TGG 0.552448 3_4:-67825714 None:intergenic
ACGACGCCCGATGCTTTGAT+GGG 0.553948 3_4:-67825443 None:intergenic
TCAAAGTAGCCAGAAAATCA+AGG 0.559172 3_4:+67824438 Msa0516330:CDS
TCTTCAGACATATCATCAGT+GGG 0.559544 3_4:-67825237 None:intergenic
CAAAGTTAGACACAAATGGT+TGG 0.564649 3_4:+67824404 Msa0516330:CDS
CTCAACTCTACAAGAGAACA+AGG 0.565237 3_4:+67822050 Msa0516330:CDS
GACTCAATCCATGCAAACAT+GGG 0.565689 3_4:+67825294 Msa0516330:CDS
ATATCTGGTGAACTATATGC+AGG 0.565870 3_4:-67823573 None:intergenic
AGACTCAATCCATGCAAACA+TGG 0.566185 3_4:+67825293 Msa0516330:CDS
CCAATTGGATTGGAACTGGT+TGG 0.567620 3_4:+67825140 Msa0516330:CDS
TGAGATTGCAAGCACTTGTG+AGG 0.571803 3_4:+67824239 Msa0516330:CDS
GACACTGATTTCAGGAAACA+TGG 0.572769 3_4:+67824460 Msa0516330:CDS
GAGATTGCAAGCACTTGTGA+GGG 0.574688 3_4:+67824240 Msa0516330:CDS
TTCAGTCAAGGGTGTTGATG+TGG 0.575945 3_4:-67823613 None:intergenic
TGGGTAAGAAAGGAAGCAGT+TGG 0.576166 3_4:+67821986 Msa0516330:CDS
TGTGTTGGGGCCATATAGCT+AGG 0.577052 3_4:-67825390 None:intergenic
TCATCAGTGGGTGTATATGG+TGG 0.580532 3_4:-67825225 None:intergenic
GCAGCATGAAGCAGCAGAGG+AGG 0.582128 3_4:+67823533 Msa0516330:CDS
CCTGTTTCTGAGATGAATGT+TGG 0.586683 3_4:-67821772 None:intergenic
CAGCACGGAGTCCTCCGACT+GGG 0.587545 3_4:+67825487 Msa0516330:CDS
TCTTACCCATCTGAGAGGAA+AGG 0.590209 3_4:-67821972 None:intergenic
CATAGCCTTTCCTCTCAGAT+GGG 0.597012 3_4:+67821967 Msa0516330:exon
GGCCCCAACACAGTCTGCAA+AGG 0.604497 3_4:+67825401 Msa0516330:CDS
AATGCGATGCATGCAAGCAT+TGG 0.605182 3_4:+67824294 Msa0516330:CDS
TTGTCCAGCCATGACCTCCA+GGG 0.606858 3_4:-67825647 None:intergenic
GGTATAGTATAATTATAAGG+AGG 0.607103 3_4:-67825693 None:intergenic
TTGGCAGCATGAAGCAGCAG+AGG 0.610256 3_4:+67823530 Msa0516330:CDS
AGTAATTGGATGAGCAGCAG+TGG 0.612150 3_4:-67825541 None:intergenic
CCACCACACACCATACATGA+AGG 0.614977 3_4:+67821738 Msa0516330:five_prime_UTR
TATGGAAGATGAGGATGGTG+AGG 0.620544 3_4:-67821640 None:intergenic
CTTACTGGAGCTCGTCAGCA+CGG 0.621539 3_4:+67825472 Msa0516330:CDS
TGTCCAGCCATGACCTCCAG+GGG 0.633773 3_4:-67825646 None:intergenic
GAATCATAGCCCATCCCAGT+CGG 0.633968 3_4:-67825501 None:intergenic
GTTGTTATGGAAGATGAGGA+TGG 0.634471 3_4:-67821645 None:intergenic
ATTGTGTGTGGTCTAAACCT+TGG 0.638387 3_4:+67825720 Msa0516330:three_prime_UTR
AAGGCCAAGATTAAGAGTCA+AGG 0.638913 3_4:+67825420 Msa0516330:CDS
CCTTCATGTATGGTGTGTGG+TGG 0.639025 3_4:-67821738 None:intergenic
CTATTACAAATACATGCCCA+AGG 0.639355 3_4:-67825737 None:intergenic
ATGCATGCAAGCATTGGTAA+GGG 0.640516 3_4:+67824300 Msa0516330:CDS
TCCTTTCTTACCCATCTGAG+AGG 0.647484 3_4:-67821977 None:intergenic
CACCAACGACACCACAGTGG+AGG 0.650201 3_4:+67825363 Msa0516330:CDS
AATTGTTGTTATGGAAGATG+AGG 0.653084 3_4:-67821649 None:intergenic
TCATAGCCCATCCCAGTCGG+AGG 0.653601 3_4:-67825498 None:intergenic
GGTCCTTCATGTATGGTGTG+TGG 0.670230 3_4:-67821741 None:intergenic
AGATTGCAAGCACTTGTGAG+GGG 0.683402 3_4:+67824241 Msa0516330:CDS
TTGTGAGGGGACATAATGTG+CGG 0.702353 3_4:+67824254 Msa0516330:CDS
ACTATACCATGTATTGTGTG+TGG 0.706301 3_4:+67825708 Msa0516330:three_prime_UTR
TGACTCACGACAAACACGAG+CGG 0.737315 3_4:+67824356 Msa0516330:CDS
CACCACCAACGACACCACAG+TGG 0.740022 3_4:+67825360 Msa0516330:CDS
GTCCAGCCATGACCTCCAGG+GGG 0.740945 3_4:-67825645 None:intergenic
AGCTAGGTACACCTCCACTG+TGG 0.765897 3_4:-67825374 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTTAATTATTACTATTTA+TGG + chr3_4:67825758-67825777 Msa0516330:three_prime_UTR 10.0%
!!! ACAATCCTATTTAATTTTTA+AGG + chr3_4:67821892-67821911 Msa0516330:intron 15.0%
!!! ATTTGAGTTAAAATTTCTTT+TGG + chr3_4:67822230-67822249 Msa0516330:intron 15.0%
!!! TTTGAGTTAAAATTTCTTTT+GGG + chr3_4:67822231-67822250 Msa0516330:intron 15.0%
!!! TATGAGAAAAATCTATTTTT+TGG - chr3_4:67822410-67822429 None:intergenic 15.0%
!! ATATAATTACATACTGTATA+GGG - chr3_4:67822724-67822743 None:intergenic 15.0%
!! TATGTAATTATATGTGTATA+TGG + chr3_4:67822731-67822750 Msa0516330:intron 15.0%
!! AATATTTGTATTAGATTGTA+CGG + chr3_4:67824057-67824076 Msa0516330:intron 15.0%
!!! TCTATTCATTTTAATTAGAT+GGG - chr3_4:67824587-67824606 None:intergenic 15.0%
!!! TTTTTACAGACAAATATTAA+TGG - chr3_4:67824858-67824877 None:intergenic 15.0%
!! CAAAACTATATTATATATTG+TGG - chr3_4:67825020-67825039 None:intergenic 15.0%
!! TGTTTATGTATCTAAATTTG+AGG - chr3_4:67821818-67821837 None:intergenic 20.0%
!! GGAAACCTTAAAAATTAAAT+AGG - chr3_4:67821900-67821919 None:intergenic 20.0%
!! CAAAAAGAAAAAAGAAAGAA+AGG - chr3_4:67822088-67822107 None:intergenic 20.0%
!!! TCTTTTTGAAAACATTTATC+AGG + chr3_4:67822100-67822119 Msa0516330:intron 20.0%
!!! TTCAGTAAACAAACAAATTT+TGG - chr3_4:67822156-67822175 None:intergenic 20.0%
!!! TGACTAAATACTGATTATTA+TGG - chr3_4:67822472-67822491 None:intergenic 20.0%
!! ATAAGTAATGCTACATATTA+TGG + chr3_4:67822568-67822587 Msa0516330:intron 20.0%
!! CATATAATTACATACTGTAT+AGG - chr3_4:67822725-67822744 None:intergenic 20.0%
!! ACTCATAATTAACAATCATT+TGG - chr3_4:67822949-67822968 None:intergenic 20.0%
!! TTTGTTAAATACAAGAGAAT+TGG + chr3_4:67824560-67824579 Msa0516330:intron 20.0%
!! TTGTTAAATACAAGAGAATT+GGG + chr3_4:67824561-67824580 Msa0516330:intron 20.0%
!!! CTCTATTCATTTTAATTAGA+TGG - chr3_4:67824588-67824607 None:intergenic 20.0%
!! ATAAAACAAGTAATATGAGT+TGG - chr3_4:67824672-67824691 None:intergenic 20.0%
!!! TATTAATGGTCAATTTGTTA+GGG - chr3_4:67824844-67824863 None:intergenic 20.0%
!!! ATATTAATGGTCAATTTGTT+AGG - chr3_4:67824845-67824864 None:intergenic 20.0%
!! CATGGTATAGTATAATTATA+AGG - chr3_4:67825699-67825718 None:intergenic 20.0%
!!! ATGCACATTTTATTAATCAA+CGG - chr3_4:67825946-67825965 None:intergenic 20.0%
!! TGAAATCCTAATTGTTGTTA+TGG - chr3_4:67821661-67821680 None:intergenic 25.0%
! GGAAGAAAAATAACATTGAT+TGG - chr3_4:67822127-67822146 None:intergenic 25.0%
!!! ACAAACAAATTTTGGTGAAT+GGG - chr3_4:67822148-67822167 None:intergenic 25.0%
!!! AACAAACAAATTTTGGTGAA+TGG - chr3_4:67822149-67822168 None:intergenic 25.0%
!!! TTTTTGAGACTTGATCAAAA+TGG - chr3_4:67822189-67822208 None:intergenic 25.0%
!! AAGCAATTTATGCTTTCTTT+TGG - chr3_4:67822539-67822558 None:intergenic 25.0%
! ATGTTATCCTTTATTGTGAA+AGG - chr3_4:67822601-67822620 None:intergenic 25.0%
! CATTTACTGTACTTATATGT+AGG - chr3_4:67822626-67822645 None:intergenic 25.0%
! TGATTGTTAATTATGAGTGA+AGG + chr3_4:67822951-67822970 Msa0516330:intron 25.0%
! ATTATGAGTGAAGGATTATT+TGG + chr3_4:67822960-67822979 Msa0516330:intron 25.0%
! TGAAATAAATTGTGTTCGTT+CGG + chr3_4:67823162-67823181 Msa0516330:intron 25.0%
! AACAATGACTTAATCTATGT+TGG + chr3_4:67823472-67823491 Msa0516330:intron 25.0%
! ACAATGACTTAATCTATGTT+GGG + chr3_4:67823473-67823492 Msa0516330:intron 25.0%
!! AAGTCTTCTTTTATCTGTTT+TGG + chr3_4:67823806-67823825 Msa0516330:intron 25.0%
! ATGAGTGTATGACATATTTA+TGG - chr3_4:67823902-67823921 None:intergenic 25.0%
! AATCAATGTGACTTTAATGT+TGG - chr3_4:67824004-67824023 None:intergenic 25.0%
!! AATACAAATATTTTCCATGC+AGG - chr3_4:67824049-67824068 None:intergenic 25.0%
! CTAAGAAAAAATGAATAACC+TGG - chr3_4:67824531-67824550 None:intergenic 25.0%
!!! ATTAATGGTCAATTTGTTAG+GGG - chr3_4:67824843-67824862 None:intergenic 25.0%
! CAAGCATCAAATATGTTAAA+AGG - chr3_4:67824914-67824933 None:intergenic 25.0%
!! CTTTTAACATATTTGATGCT+TGG + chr3_4:67824912-67824931 Msa0516330:intron 25.0%
! TTTCACTATCATCAACATTT+GGG - chr3_4:67825095-67825114 None:intergenic 25.0%
! TATCACAATCAAACAATGTT+AGG + chr3_4:67825175-67825194 Msa0516330:CDS 25.0%
! GACTTAAATCATGAAATTCT+TGG - chr3_4:67825329-67825348 None:intergenic 25.0%
! GGTATAGTATAATTATAAGG+AGG - chr3_4:67825696-67825715 None:intergenic 25.0%
!! GAATCATAATATTCGTTTTC+GGG - chr3_4:67825829-67825848 None:intergenic 25.0%
!! GGAATCATAATATTCGTTTT+CGG - chr3_4:67825830-67825849 None:intergenic 25.0%
!! TGCACATTTTATTAATCAAC+GGG - chr3_4:67825945-67825964 None:intergenic 25.0%
! AATTGTTGTTATGGAAGATG+AGG - chr3_4:67821652-67821671 None:intergenic 30.0%
CATCTTCCATAACAACAATT+AGG + chr3_4:67821652-67821671 Msa0516330:five_prime_UTR 30.0%
CATTCTCTTCGTAACATATA+AGG + chr3_4:67821836-67821855 Msa0516330:intron 30.0%
!!! AGTTTTCAAGTCTTCTTCTA+AGG + chr3_4:67822026-67822045 Msa0516330:CDS 30.0%
TCTCATAGACAATAAGAATC+TGG + chr3_4:67822423-67822442 Msa0516330:intron 30.0%
!!! TATGCTTTCTTTTGGTTTCT+TGG - chr3_4:67822531-67822550 None:intergenic 30.0%
! GTTGTAGTTGTTGTCTTTTA+TGG + chr3_4:67822655-67822674 Msa0516330:intron 30.0%
! TCTTTTATGGAACCACATAA+AGG + chr3_4:67822668-67822687 Msa0516330:intron 30.0%
ATGTTAAGTTCACATGATAG+GGG - chr3_4:67822758-67822777 None:intergenic 30.0%
CATGTTAAGTTCACATGATA+GGG - chr3_4:67822759-67822778 None:intergenic 30.0%
ACATGTTAAGTTCACATGAT+AGG - chr3_4:67822760-67822779 None:intergenic 30.0%
CACTATGGAAATCTAACATT+GGG - chr3_4:67822872-67822891 None:intergenic 30.0%
TAATTAAGTGATTAACCAGC+TGG - chr3_4:67822909-67822928 None:intergenic 30.0%
! TTTTCACTTTCAGAAGGAAA+AGG + chr3_4:67823500-67823519 Msa0516330:intron 30.0%
CAGATAAAAGAAGACTTACT+AGG - chr3_4:67823803-67823822 None:intergenic 30.0%
ATTAATTCTGTGGTTCAGTA+TGG + chr3_4:67823875-67823894 Msa0516330:intron 30.0%
GTAACTGAAAGTTTGAAAGT+AGG - chr3_4:67823926-67823945 None:intergenic 30.0%
TGACACTTGCAATATATGAT+CGG + chr3_4:67823966-67823985 Msa0516330:intron 30.0%
TAATTGATTGAGTGTGTTCA+GGG - chr3_4:67824098-67824117 None:intergenic 30.0%
TTAATTGATTGAGTGTGTTC+AGG - chr3_4:67824099-67824118 None:intergenic 30.0%
AGCTAATGACAGAAAAACAT+AGG - chr3_4:67824145-67824164 None:intergenic 30.0%
TTTGTGTCTAACTTTGTAGT+AGG - chr3_4:67824400-67824419 None:intergenic 30.0%
ACTACAAAGTTAGACACAAA+TGG + chr3_4:67824400-67824419 Msa0516330:CDS 30.0%
AAAATGAATAGAGTGTAGAG+TGG + chr3_4:67824595-67824614 Msa0516330:intron 30.0%
AAGTTAGTTGCTCAATTTAG+AGG + chr3_4:67824698-67824717 Msa0516330:intron 30.0%
!! TTAATGGTCAATTTGTTAGG+GGG - chr3_4:67824842-67824861 None:intergenic 30.0%
CTTTCACTATCATCAACATT+TGG - chr3_4:67825096-67825115 None:intergenic 30.0%
TGAAGAAAAAACAGTTGACA+TGG + chr3_4:67825254-67825273 Msa0516330:CDS 30.0%
ATTCATGGGACAGTAATGTT+TGG - chr3_4:67821708-67821727 None:intergenic 35.0%
!!! GTGGCATTTTTGAGATTCAT+GGG - chr3_4:67821722-67821741 None:intergenic 35.0%
!! AGATGAATGTTGGTAGTTAC+TGG - chr3_4:67821765-67821784 None:intergenic 35.0%
! GGATTGTTACCTTGTCTTTT+AGG - chr3_4:67821879-67821898 None:intergenic 35.0%
CAATTCAGACAACATGTTAG+AGG - chr3_4:67821921-67821940 None:intergenic 35.0%
TGTAGCATTACTTATACTGC+AGG - chr3_4:67822563-67822582 None:intergenic 35.0%
CAGTGTACCTTTCACAATAA+AGG + chr3_4:67822591-67822610 Msa0516330:intron 35.0%
AGGTAGATTCATCCTTTATG+TGG - chr3_4:67822683-67822702 None:intergenic 35.0%
GATGAATGATGTGATGTGTT+AGG - chr3_4:67822832-67822851 None:intergenic 35.0%
GCACTATGGAAATCTAACAT+TGG - chr3_4:67822873-67822892 None:intergenic 35.0%
! GATGGATTTGTTTAGCACTA+TGG - chr3_4:67822887-67822906 None:intergenic 35.0%
GAGTGAAGGATTATTTGGAT+CGG + chr3_4:67822965-67822984 Msa0516330:intron 35.0%
TAAACACTGATGAGACTGTT+TGG + chr3_4:67823012-67823031 Msa0516330:intron 35.0%
AAACACTGATGAGACTGTTT+GGG + chr3_4:67823013-67823032 Msa0516330:intron 35.0%
CATATAGTCGTCTATTTGGA+TGG - chr3_4:67823429-67823448 None:intergenic 35.0%
AGTGCATATAGTCGTCTATT+TGG - chr3_4:67823433-67823452 None:intergenic 35.0%
!!! GGACTTTTTTCACTTTCAGA+AGG + chr3_4:67823494-67823513 Msa0516330:intron 35.0%
AGAAGGAAAAGGAAGAGAAT+TGG + chr3_4:67823511-67823530 Msa0516330:intron 35.0%
ATATCTGGTGAACTATATGC+AGG - chr3_4:67823576-67823595 None:intergenic 35.0%
TGATTTCTCTCTTCAGTCAA+GGG - chr3_4:67823627-67823646 None:intergenic 35.0%
ATGATTTCTCTCTTCAGTCA+AGG - chr3_4:67823628-67823647 None:intergenic 35.0%
TACTAGGTATCCTCTATAGT+AGG - chr3_4:67823787-67823806 None:intergenic 35.0%
GCAGCATCATATTAATTCTG+TGG + chr3_4:67823865-67823884 Msa0516330:intron 35.0%
ATCATTGTAGTAAACCTGCA+TGG + chr3_4:67824032-67824051 Msa0516330:intron 35.0%
AGCTGTTCCATTTCGTATAT+AGG + chr3_4:67824183-67824202 Msa0516330:intron 35.0%
CAAAGTTAGACACAAATGGT+TGG + chr3_4:67824404-67824423 Msa0516330:CDS 35.0%
ACACAAATGGTTGGAACTAT+AGG + chr3_4:67824413-67824432 Msa0516330:CDS 35.0%
TCAAAGTAGCCAGAAAATCA+AGG + chr3_4:67824438-67824457 Msa0516330:CDS 35.0%
! AATCAGTGTCCTTGATTTTC+TGG - chr3_4:67824450-67824469 None:intergenic 35.0%
!! AAATCAAGGACACTGATTTC+AGG + chr3_4:67824452-67824471 Msa0516330:CDS 35.0%
ACATGGAGCTACAATGAATA+AGG + chr3_4:67824477-67824496 Msa0516330:CDS 35.0%
!! CTAAGGAAGTATTGTGAAGT+AGG + chr3_4:67824785-67824804 Msa0516330:intron 35.0%
!! TAATGGTCAATTTGTTAGGG+GGG - chr3_4:67824841-67824860 None:intergenic 35.0%
! TGTGGTAATGATTCTTCAGA+TGG - chr3_4:67825002-67825021 None:intergenic 35.0%
TCAAACAATGTTAGGCCTTT+AGG + chr3_4:67825183-67825202 Msa0516330:CDS 35.0%
CAAACAATGTTAGGCCTTTA+GGG + chr3_4:67825184-67825203 Msa0516330:CDS 35.0%
ATATCATCAGTGGGTGTATA+TGG - chr3_4:67825231-67825250 None:intergenic 35.0%
TCTTCAGACATATCATCAGT+GGG - chr3_4:67825240-67825259 None:intergenic 35.0%
TTCTTCAGACATATCATCAG+TGG - chr3_4:67825241-67825260 None:intergenic 35.0%
AAAAACAGTTGACATGGACA+TGG + chr3_4:67825260-67825279 Msa0516330:CDS 35.0%
CATGAAATTCTTGGTTCATG+AGG - chr3_4:67825320-67825339 None:intergenic 35.0%
!! TCCCATTAATTTTTGGGCTT+GGG - chr3_4:67825572-67825591 None:intergenic 35.0%
!! ATCCCATTAATTTTTGGGCT+TGG - chr3_4:67825573-67825592 None:intergenic 35.0%
! GGCGAATCCCATTAATTTTT+GGG - chr3_4:67825578-67825597 None:intergenic 35.0%
! AGGCGAATCCCATTAATTTT+TGG - chr3_4:67825579-67825598 None:intergenic 35.0%
! CCTTCAATCTAAACGGATTT+CGG + chr3_4:67825596-67825615 Msa0516330:CDS 35.0%
! CTTCAATCTAAACGGATTTC+GGG + chr3_4:67825597-67825616 Msa0516330:CDS 35.0%
ACTATACCATGTATTGTGTG+TGG + chr3_4:67825708-67825727 Msa0516330:three_prime_UTR 35.0%
TTTAGACCACACACAATACA+TGG - chr3_4:67825717-67825736 None:intergenic 35.0%
CTATTACAAATACATGCCCA+AGG - chr3_4:67825740-67825759 None:intergenic 35.0%
TGACAGTGACACAAAACATA+TGG - chr3_4:67825851-67825870 None:intergenic 35.0%
GTTGTTATGGAAGATGAGGA+TGG - chr3_4:67821648-67821667 None:intergenic 40.0%
!!! GGTGGCATTTTTGAGATTCA+TGG - chr3_4:67821723-67821742 None:intergenic 40.0%
AGTTACTGGTCCTTCATGTA+TGG - chr3_4:67821751-67821770 None:intergenic 40.0%
!! CCTGTTTCTGAGATGAATGT+TGG - chr3_4:67821775-67821794 None:intergenic 40.0%
CCAACATTCATCTCAGAAAC+AGG + chr3_4:67821772-67821791 Msa0516330:five_prime_UTR 40.0%
CGTGTATGTCCTAAAAGACA+AGG + chr3_4:67821867-67821886 Msa0516330:intron 40.0%
CTCAACTCTACAAGAGAACA+AGG + chr3_4:67822050-67822069 Msa0516330:CDS 40.0%
GATGAATCTACCTTGCCTTT+AGG + chr3_4:67822690-67822709 Msa0516330:intron 40.0%
TACATACTGTATAGGGTTGC+TGG - chr3_4:67822717-67822736 None:intergenic 40.0%
TAAGTGATTAACCAGCTGGA+TGG - chr3_4:67822905-67822924 None:intergenic 40.0%
! GACTAATTCCATTAGCTCTC+TGG + chr3_4:67823074-67823093 Msa0516330:intron 40.0%
TAAGCTATCCAGAGAGCTAA+TGG - chr3_4:67823085-67823104 None:intergenic 40.0%
TCACCAGATATCACGAATGA+AGG + chr3_4:67823585-67823604 Msa0516330:CDS 40.0%
CTTCCTTCATTCGTGATATC+TGG - chr3_4:67823591-67823610 None:intergenic 40.0%
AGGTCATGGACATAACTCTA+AGG + chr3_4:67823734-67823753 Msa0516330:CDS 40.0%
CTCATTCAGTCCTACTATAG+AGG + chr3_4:67823774-67823793 Msa0516330:CDS 40.0%
!! ATGCATGCAAGCATTGGTAA+GGG + chr3_4:67824300-67824319 Msa0516330:CDS 40.0%
!! GACACTGATTTCAGGAAACA+TGG + chr3_4:67824460-67824479 Msa0516330:CDS 40.0%
! GCTTGGTGACTACACAAAAA+CGG + chr3_4:67824929-67824948 Msa0516330:intron 40.0%
GCATAGAAAACCACATGCAA+AGG + chr3_4:67824968-67824987 Msa0516330:intron 40.0%
! GGAATTGATTCCTTTGCATG+TGG - chr3_4:67824981-67825000 None:intergenic 40.0%
CAACCAGTTCCAATCCAATT+GGG - chr3_4:67825142-67825161 None:intergenic 40.0%
!! AATGTTAGGCCTTTAGGGTT+GGG + chr3_4:67825189-67825208 Msa0516330:CDS 40.0%
! TTTGCATGGATTGAGTCTCT+TGG - chr3_4:67825291-67825310 None:intergenic 40.0%
AGACTCAATCCATGCAAACA+TGG + chr3_4:67825293-67825312 Msa0516330:CDS 40.0%
GACTCAATCCATGCAAACAT+GGG + chr3_4:67825294-67825313 Msa0516330:CDS 40.0%
AAGGCCAAGATTAAGAGTCA+AGG + chr3_4:67825420-67825439 Msa0516330:CDS 40.0%
AGCCCAAGCCCAAAAATTAA+TGG + chr3_4:67825567-67825586 Msa0516330:CDS 40.0%
GCCCAAGCCCAAAAATTAAT+GGG + chr3_4:67825568-67825587 Msa0516330:CDS 40.0%
GGATTCGCCTTCAATCTAAA+CGG + chr3_4:67825589-67825608 Msa0516330:CDS 40.0%
CCGAAATCCGTTTAGATTGA+AGG - chr3_4:67825599-67825618 None:intergenic 40.0%
ATTGTGTGTGGTCTAAACCT+TGG + chr3_4:67825720-67825739 Msa0516330:three_prime_UTR 40.0%
TTGTGTGTGGTCTAAACCTT+GGG + chr3_4:67825721-67825740 Msa0516330:three_prime_UTR 40.0%
!!! ATTCGTTTTCGGGTGTCTTT+TGG - chr3_4:67825819-67825838 None:intergenic 40.0%
TATGGAAGATGAGGATGGTG+AGG - chr3_4:67821643-67821662 None:intergenic 45.0%
ACATAGCCTTTCCTCTCAGA+TGG + chr3_4:67821966-67821985 Msa0516330:exon 45.0%
CATAGCCTTTCCTCTCAGAT+GGG + chr3_4:67821967-67821986 Msa0516330:exon 45.0%
TCTTACCCATCTGAGAGGAA+AGG - chr3_4:67821975-67821994 None:intergenic 45.0%
TCCTCTCAGATGGGTAAGAA+AGG + chr3_4:67821976-67821995 Msa0516330:exon 45.0%
TCCTTTCTTACCCATCTGAG+AGG - chr3_4:67821980-67821999 None:intergenic 45.0%
TGGGTAAGAAAGGAAGCAGT+TGG + chr3_4:67821986-67822005 Msa0516330:CDS 45.0%
TATAGGGTTGCTGGTCCTAA+AGG - chr3_4:67822708-67822727 None:intergenic 45.0%
GCTAAACAAATCCATCCAGC+TGG + chr3_4:67822891-67822910 Msa0516330:intron 45.0%
!! TTCAGTCAAGGGTGTTGATG+TGG - chr3_4:67823616-67823635 None:intergenic 45.0%
AGCTGCTAGAGTTGTGAGAT+TGG + chr3_4:67823710-67823729 Msa0516330:CDS 45.0%
CGTCGAGCCTATATACGAAA+TGG - chr3_4:67824193-67824212 None:intergenic 45.0%
TGAGATTGCAAGCACTTGTG+AGG + chr3_4:67824239-67824258 Msa0516330:CDS 45.0%
GAGATTGCAAGCACTTGTGA+GGG + chr3_4:67824240-67824259 Msa0516330:CDS 45.0%
AGATTGCAAGCACTTGTGAG+GGG + chr3_4:67824241-67824260 Msa0516330:CDS 45.0%
TTGTGAGGGGACATAATGTG+CGG + chr3_4:67824254-67824273 Msa0516330:CDS 45.0%
AATGCGATGCATGCAAGCAT+TGG + chr3_4:67824294-67824313 Msa0516330:CDS 45.0%
!! GATGCATGCAAGCATTGGTA+AGG + chr3_4:67824299-67824318 Msa0516330:CDS 45.0%
CGTGTTTGTCGTGAGTCAAT+TGG - chr3_4:67824354-67824373 None:intergenic 45.0%
ACCTTTCATGTAGATCCCGA+TGG + chr3_4:67824734-67824753 Msa0516330:intron 45.0%
ACCATCGGGATCTACATGAA+AGG - chr3_4:67824738-67824757 None:intergenic 45.0%
GACTACATCAGTACACCATC+GGG - chr3_4:67824752-67824771 None:intergenic 45.0%
CGACTACATCAGTACACCAT+CGG - chr3_4:67824753-67824772 None:intergenic 45.0%
! CGATGGTGTACTGATGTAGT+CGG + chr3_4:67824751-67824770 Msa0516330:intron 45.0%
TACTGATGTAGTCGGTCGTT+AGG + chr3_4:67824759-67824778 Msa0516330:intron 45.0%
GTGAAAGAGCCCAATTGGAT+TGG + chr3_4:67825130-67825149 Msa0516330:CDS 45.0%
CCAACCAGTTCCAATCCAAT+TGG - chr3_4:67825143-67825162 None:intergenic 45.0%
! CCAATTGGATTGGAACTGGT+TGG + chr3_4:67825140-67825159 Msa0516330:CDS 45.0%
!! CAATGTTAGGCCTTTAGGGT+TGG + chr3_4:67825188-67825207 Msa0516330:CDS 45.0%
!! ATGTTAGGCCTTTAGGGTTG+GGG + chr3_4:67825190-67825209 Msa0516330:CDS 45.0%
ATCAGTGGGTGTATATGGTG+GGG - chr3_4:67825226-67825245 None:intergenic 45.0%
CATCAGTGGGTGTATATGGT+GGG - chr3_4:67825227-67825246 None:intergenic 45.0%
TCATCAGTGGGTGTATATGG+TGG - chr3_4:67825228-67825247 None:intergenic 45.0%
!! CGTTGGTGGTGTTTAGAGAT+AGG - chr3_4:67825351-67825370 None:intergenic 45.0%
! TGGAGGTGTACCTAGCTATA+TGG + chr3_4:67825380-67825399 Msa0516330:CDS 45.0%
TGGGCCTTGACTCTTAATCT+TGG - chr3_4:67825427-67825446 None:intergenic 45.0%
!! TATGATTCATCTGGCTCTGG+TGG + chr3_4:67825516-67825535 Msa0516330:CDS 45.0%
!! ATGATTCATCTGGCTCTGGT+GGG + chr3_4:67825517-67825536 Msa0516330:CDS 45.0%
AGTAATTGGATGAGCAGCAG+TGG - chr3_4:67825544-67825563 None:intergenic 45.0%
!! TTTCGGGTGTCTTTTGGTGT+GGG - chr3_4:67825813-67825832 None:intergenic 45.0%
!!! TTTTCGGGTGTCTTTTGGTG+TGG - chr3_4:67825814-67825833 None:intergenic 45.0%
CCTTCATGTATGGTGTGTGG+TGG - chr3_4:67821741-67821760 None:intergenic 50.0%
CCACCACACACCATACATGA+AGG + chr3_4:67821738-67821757 Msa0516330:five_prime_UTR 50.0%
GGTCCTTCATGTATGGTGTG+TGG - chr3_4:67821744-67821763 None:intergenic 50.0%
CTGCGAGAGCTTACGGAAAT+GGG - chr3_4:67823365-67823384 None:intergenic 50.0%
TCTGCGAGAGCTTACGGAAA+TGG - chr3_4:67823366-67823385 None:intergenic 50.0%
AGACTGTCTGCGAGAGCTTA+CGG - chr3_4:67823372-67823391 None:intergenic 50.0%
!! AGCAGCAGAGGAGGTATCTA+TGG + chr3_4:67823542-67823561 Msa0516330:CDS 50.0%
GCTAGAGTTGTGAGATTGGC+AGG + chr3_4:67823714-67823733 Msa0516330:CDS 50.0%
GTTGTGAGATTGGCAGGTCA+TGG + chr3_4:67823720-67823739 Msa0516330:CDS 50.0%
!! GCAAGCATTGGTAAGGGTTC+AGG + chr3_4:67824306-67824325 Msa0516330:CDS 50.0%
TGACTCACGACAAACACGAG+CGG + chr3_4:67824356-67824375 Msa0516330:CDS 50.0%
TCCATACGCTTTCAACTGCC+AGG + chr3_4:67824510-67824529 Msa0516330:CDS 50.0%
! ACCTGGCAGTTGAAAGCGTA+TGG - chr3_4:67824514-67824533 None:intergenic 50.0%
AGTCGGTCGTTAGGATGCTA+AGG + chr3_4:67824768-67824787 Msa0516330:intron 50.0%
!! TGGTCAATTTGTTAGGGGGG+TGG - chr3_4:67824838-67824857 None:intergenic 50.0%
TGAGCGTGAAAGAGCCCAAT+TGG + chr3_4:67825125-67825144 Msa0516330:CDS 50.0%
! GAGCCCAATTGGATTGGAAC+TGG + chr3_4:67825136-67825155 Msa0516330:CDS 50.0%
ATTGGAACTGGTTGGAGCGT+TGG + chr3_4:67825148-67825167 Msa0516330:CDS 50.0%
! GTATATGGTGGGGTCTCAAG+TGG - chr3_4:67825216-67825235 None:intergenic 50.0%
TCATGAGGCCCATGTTTGCA+TGG - chr3_4:67825305-67825324 None:intergenic 50.0%
TGTGTTGGGGCCATATAGCT+AGG - chr3_4:67825393-67825412 None:intergenic 50.0%
TGGCCTTTGCAGACTGTGTT+GGG - chr3_4:67825407-67825426 None:intergenic 50.0%
! TTGGCCTTTGCAGACTGTGT+TGG - chr3_4:67825408-67825427 None:intergenic 50.0%
GTCAAGGCCCATCAAAGCAT+CGG + chr3_4:67825436-67825455 Msa0516330:CDS 50.0%
! TCAAGGCCCATCAAAGCATC+GGG + chr3_4:67825437-67825456 Msa0516330:CDS 50.0%
GAATCATAGCCCATCCCAGT+CGG - chr3_4:67825504-67825523 None:intergenic 50.0%
!! GGGATGGGCTATGATTCATC+TGG + chr3_4:67825507-67825526 Msa0516330:CDS 50.0%
! GGCTATGATTCATCTGGCTC+TGG + chr3_4:67825513-67825532 Msa0516330:CDS 50.0%
!! TGATTCATCTGGCTCTGGTG+GGG + chr3_4:67825518-67825537 Msa0516330:CDS 50.0%
GGGCTTGGGCTTCTAGTAAT+TGG - chr3_4:67825558-67825577 None:intergenic 50.0%
! TCTAAACGGATTTCGGGTGC+TGG + chr3_4:67825603-67825622 Msa0516330:CDS 50.0%
!! TTCGGGTGTCTTTTGGTGTG+GGG - chr3_4:67825812-67825831 None:intergenic 50.0%
GGTTGCTGGTCCTAAAGGCA+AGG - chr3_4:67822703-67822722 None:intergenic 55.0%
TTGGCAGCATGAAGCAGCAG+AGG + chr3_4:67823530-67823549 Msa0516330:CDS 55.0%
TGCTGCTGTTGCTGCAGCAT+AGG - chr3_4:67823658-67823677 None:intergenic 55.0%
ACGTGCACTGCGTGCTTTGA+AGG + chr3_4:67824210-67824229 Msa0516330:CDS 55.0%
! CGTGCACTGCGTGCTTTGAA+GGG + chr3_4:67824211-67824230 Msa0516330:CDS 55.0%
CAAACACGAGCGGACAGTAG+AGG + chr3_4:67824366-67824385 Msa0516330:CDS 55.0%
TCAAGTGGCCCCAACCCTAA+AGG - chr3_4:67825201-67825220 None:intergenic 55.0%
AGCTAGGTACACCTCCACTG+TGG - chr3_4:67825377-67825396 None:intergenic 55.0%
GGCCTTTGCAGACTGTGTTG+GGG - chr3_4:67825406-67825425 None:intergenic 55.0%
ACGACGCCCGATGCTTTGAT+GGG - chr3_4:67825446-67825465 None:intergenic 55.0%
AACGACGCCCGATGCTTTGA+TGG - chr3_4:67825447-67825466 None:intergenic 55.0%
!! TCAAAGCATCGGGCGTCGTT+TGG + chr3_4:67825447-67825466 Msa0516330:CDS 55.0%
! GGGCGTCGTTTGGATCTTAC+TGG + chr3_4:67825457-67825476 Msa0516330:CDS 55.0%
CTTACTGGAGCTCGTCAGCA+CGG + chr3_4:67825472-67825491 Msa0516330:CDS 55.0%
!! GATTTCGGGTGCTGGTGTTG+AGG + chr3_4:67825611-67825630 Msa0516330:CDS 55.0%
TTGTCCAGCCATGACCTCCA+GGG - chr3_4:67825650-67825669 None:intergenic 55.0%
GCAGCATGAAGCAGCAGAGG+AGG + chr3_4:67823533-67823552 Msa0516330:CDS 60.0%
AGCAGCAGCTGAAGCTGCAG+TGG + chr3_4:67823674-67823693 Msa0516330:CDS 60.0%
AGCTGAAGCTGCAGTGGCTG+CGG + chr3_4:67823680-67823699 Msa0516330:CDS 60.0%
CACCACCAACGACACCACAG+TGG + chr3_4:67825360-67825379 Msa0516330:CDS 60.0%
!! CTCCACTGTGGTGTCGTTGG+TGG - chr3_4:67825365-67825384 None:intergenic 60.0%
CACCAACGACACCACAGTGG+AGG + chr3_4:67825363-67825382 Msa0516330:CDS 60.0%
!! CACCTCCACTGTGGTGTCGT+TGG - chr3_4:67825368-67825387 None:intergenic 60.0%
GGCCCCAACACAGTCTGCAA+AGG + chr3_4:67825401-67825420 Msa0516330:CDS 60.0%
TCATAGCCCATCCCAGTCGG+AGG - chr3_4:67825501-67825520 None:intergenic 60.0%
!! CGGGTGCTGGTGTTGAGGAT+TGG + chr3_4:67825616-67825635 Msa0516330:CDS 60.0%
!! GAGGATTGGCCAATTCCCCC+TGG + chr3_4:67825630-67825649 Msa0516330:CDS 60.0%
! GATTGGCCAATTCCCCCTGG+AGG + chr3_4:67825633-67825652 Msa0516330:CDS 60.0%
CCATGACCTCCAGGGGGAAT+TGG - chr3_4:67825642-67825661 None:intergenic 60.0%
CCAATTCCCCCTGGAGGTCA+TGG + chr3_4:67825639-67825658 Msa0516330:CDS 60.0%
TGTCCAGCCATGACCTCCAG+GGG - chr3_4:67825649-67825668 None:intergenic 60.0%
CTTGTCCAGCCATGACCTCC+AGG - chr3_4:67825651-67825670 None:intergenic 60.0%
TCAGCACGGAGTCCTCCGAC+TGG + chr3_4:67825486-67825505 Msa0516330:CDS 65.0%
CAGCACGGAGTCCTCCGACT+GGG + chr3_4:67825487-67825506 Msa0516330:CDS 65.0%
ACGGAGTCCTCCGACTGGGA+TGG + chr3_4:67825491-67825510 Msa0516330:CDS 65.0%
CGGAGTCCTCCGACTGGGAT+GGG + chr3_4:67825492-67825511 Msa0516330:CDS 65.0%
TTCCCCCTGGAGGTCATGGC+TGG + chr3_4:67825643-67825662 Msa0516330:CDS 65.0%
GTCCAGCCATGACCTCCAGG+GGG - chr3_4:67825648-67825667 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 67821589 67825971 67821589 ID=Msa0516330;Name=Msa0516330
chr3_4 mRNA 67821589 67825971 67821589 ID=Msa0516330-mRNA-1;Parent=Msa0516330;Name=Msa0516330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=226|1|1|1|0.75|0.6|5|291|440
chr3_4 exon 67821589 67821793 67821589 ID=Msa0516330-mRNA-1:exon:17736;Parent=Msa0516330-mRNA-1
chr3_4 exon 67821964 67822071 67821964 ID=Msa0516330-mRNA-1:exon:17737;Parent=Msa0516330-mRNA-1
chr3_4 exon 67823513 67823803 67823513 ID=Msa0516330-mRNA-1:exon:17738;Parent=Msa0516330-mRNA-1
chr3_4 exon 67824205 67824531 67824205 ID=Msa0516330-mRNA-1:exon:17739;Parent=Msa0516330-mRNA-1
chr3_4 exon 67825063 67825971 67825063 ID=Msa0516330-mRNA-1:exon:17740;Parent=Msa0516330-mRNA-1
chr3_4 five_prime_UTR 67821589 67821793 67821589 ID=Msa0516330-mRNA-1:five_prime_utr;Parent=Msa0516330-mRNA-1
chr3_4 five_prime_UTR 67821964 67821984 67821964 ID=Msa0516330-mRNA-1:five_prime_utr;Parent=Msa0516330-mRNA-1
chr3_4 CDS 67821985 67822071 67821985 ID=Msa0516330-mRNA-1:cds;Parent=Msa0516330-mRNA-1
chr3_4 CDS 67823513 67823803 67823513 ID=Msa0516330-mRNA-1:cds;Parent=Msa0516330-mRNA-1
chr3_4 CDS 67824205 67824531 67824205 ID=Msa0516330-mRNA-1:cds;Parent=Msa0516330-mRNA-1
chr3_4 CDS 67825063 67825680 67825063 ID=Msa0516330-mRNA-1:cds;Parent=Msa0516330-mRNA-1
chr3_4 three_prime_UTR 67825681 67825971 67825681 ID=Msa0516330-mRNA-1:three_prime_utr;Parent=Msa0516330-mRNA-1
Gene Sequence

>Msa0516330

ATGGGTAAGAAAGGAAGCAGTTGGTTCTCATCTGTGAAGAAAGTTTTCAAGTCTTCTTCTAAGGACTCAACTCTACAAGAGAACAAGAAGGAAAAGGAAGAGAATTGGCAGCATGAAGCAGCAGAGGAGGTATCTATGGAGCATTTTCCTGCATATAGTTCACCAGATATCACGAATGAAGGAAGCACCACATCAACACCCTTGACTGAAGAGAGAAATCATGCAATTGCCTATGCTGCAGCAACAGCAGCAGCAGCTGAAGCTGCAGTGGCTGCGGCTCAAGCAGCTGCTAGAGTTGTGAGATTGGCAGGTCATGGACATAACTCTAAGGTTGAAAAAGCAGCAATACTCATTCAGTCCTACTATAGAGGATACCTAGCTCGACGTGCACTGCGTGCTTTGAAGGGATTAGTGAGATTGCAAGCACTTGTGAGGGGACATAATGTGCGGAAGCAAGCACAGATGACAATGCGATGCATGCAAGCATTGGTAAGGGTTCAGGCAAGAGTAAGAGCTCGCAGACTCCAATTGACTCACGACAAACACGAGCGGACAGTAGAGGAACAACATCCTACTACAAAGTTAGACACAAATGGTTGGAACTATAGGCGTCAAAGTAGCCAGAAAATCAAGGACACTGATTTCAGGAAACATGGAGCTACAATGAATAAGGAGAAAGCTCTTCCATACGCTTTCAACTGCCAGCAACTGCAGAAGCAATATTTGCACATAGACCCAAATGTTGATGATAGTGAAAGTTACAGCAATGAGCGTGAAAGAGCCCAATTGGATTGGAACTGGTTGGAGCGTTGGATGTTATCACAATCAAACAATGTTAGGCCTTTAGGGTTGGGGCCACTTGAGACCCCACCATATACACCCACTGATGATATGTCTGAAGAAAAAACAGTTGACATGGACATGGTTGCACCAAGAGACTCAATCCATGCAAACATGGGCCTCATGAACCAAGAATTTCATGATTTAAGTCCTATCTCTAAACACCACCAACGACACCACAGTGGAGGTGTACCTAGCTATATGGCCCCAACACAGTCTGCAAAGGCCAAGATTAAGAGTCAAGGCCCATCAAAGCATCGGGCGTCGTTTGGATCTTACTGGAGCTCGTCAGCACGGAGTCCTCCGACTGGGATGGGCTATGATTCATCTGGCTCTGGTGGGGCCACTGCTGCTCATCCAATTACTAGAAGCCCAAGCCCAAAAATTAATGGGATTCGCCTTCAATCTAAACGGATTTCGGGTGCTGGTGTTGAGGATTGGCCAATTCCCCCTGGAGGTCATGGCTGGACAAGATTTGATTGA
Protein sequence

>Msa0516330

MGKKGSSWFSSVKKVFKSSSKDSTLQENKKEKEENWQHEAAEEVSMEHFPAYSSPDITNEGSTTSTPLTEERNHAIAYAAATAAAAEAAVAAAQAAARVVRLAGHGHNSKVEKAAILIQSYYRGYLARRALRALKGLVRLQALVRGHNVRKQAQMTMRCMQALVRVQARVRARRLQLTHDKHERTVEEQHPTTKLDTNGWNYRRQSSQKIKDTDFRKHGATMNKEKALPYAFNCQQLQKQYLHIDPNVDDSESYSNERERAQLDWNWLERWMLSQSNNVRPLGLGPLETPPYTPTDDMSEEKTVDMDMVAPRDSIHANMGLMNQEFHDLSPISKHHQRHHSGGVPSYMAPTQSAKAKIKSQGPSKHRASFGSYWSSSARSPPTGMGYDSSGSGGATAAHPITRSPSPKINGIRLQSKRISGAGVEDWPIPPGGHGWTRFD*