AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82878


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82878.t1 MTR_6g006060 96.133 181 6 1 1 180 1 181 4.23e-119 334
MS.gene82878.t1 MTR_8g076780 31.937 191 102 5 14 179 7 194 1.74e-16 73.9
MS.gene82878.t1 MTR_7g053520 34.351 131 76 2 59 179 86 216 1.29e-14 69.3
MS.gene82878.t1 MTR_2g031960 32.867 143 86 3 47 179 12 154 4.18e-14 66.6
MS.gene82878.t1 MTR_2g031760 49.254 67 34 0 109 175 119 185 2.69e-13 65.5
MS.gene82878.t1 MTR_2g064300 33.333 132 76 2 59 179 85 215 6.20e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82878.t1 AT3G27850 59.239 184 69 3 1 180 6 187 1.76e-52 166
MS.gene82878.t1 AT3G27830 69.231 143 40 2 42 180 49 191 6.14e-51 162
MS.gene82878.t1 AT3G27830 69.231 143 40 2 42 180 49 191 6.14e-51 162
MS.gene82878.t1 AT3G27840 50.898 167 64 3 31 179 26 192 5.79e-44 144
MS.gene82878.t1 AT4G36420 36.301 146 79 3 48 179 33 178 5.33e-18 77.4
MS.gene82878.t1 AT3G06040 46.667 75 40 0 101 175 107 181 5.32e-13 64.3
MS.gene82878.t1 AT3G06040 46.667 75 40 0 101 175 107 181 5.32e-13 64.3
MS.gene82878.t1 AT3G06040 46.667 75 40 0 101 175 107 181 5.32e-13 64.3
MS.gene82878.t1 AT1G70190 34.074 135 75 2 59 179 73 207 9.49e-13 64.3
MS.gene82878.t1 AT1G70190 34.074 135 75 2 59 179 73 207 9.49e-13 64.3
MS.gene82878.t1 AT4G37660 32.258 155 92 3 38 179 12 166 1.31e-12 63.2

Find 52 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTAGAGCTTTAACGAGTT+TGG 0.267140 6.1:-1575594 MS.gene82878:CDS
GTGGGAGATGGAGAGGGGTT+TGG 0.285418 6.1:+1575886 None:intergenic
GAGGTAGTCGACGAGGTCTT+TGG 0.313141 6.1:+1575772 None:intergenic
TTCAATTACCACATCGAATT+CGG 0.338695 6.1:+1575655 None:intergenic
TTCTTCGACTACTGCGACTT+CGG 0.340678 6.1:+1575682 None:intergenic
GGAGATGGAGAGGGGTTTGG+AGG 0.359975 6.1:+1575889 None:intergenic
GTTAGAGCTTTAACGAGTTT+GGG 0.364862 6.1:-1575593 MS.gene82878:CDS
AGATGGAGAGGGGTTTGGAG+GGG 0.389473 6.1:+1575891 None:intergenic
ACACCTTCTTTAAATTTCTT+CGG 0.390394 6.1:+1575536 None:intergenic
GGAGACGAGTGCGGTGGTGG+TGG 0.390691 6.1:+1575867 None:intergenic
GGTGGTGGTGGGAGATGGAG+AGG 0.392248 6.1:+1575879 None:intergenic
TGGTGGTGGGAGATGGAGAG+GGG 0.394112 6.1:+1575881 None:intergenic
AGCGGCGACAGTGGCGGCGG+CGG 0.407686 6.1:+1575712 None:intergenic
GCCGCAATTCTCGCGTTACT+AGG 0.408930 6.1:+1575626 None:intergenic
GTCGACGAGGTCTTTGGCTT+GGG 0.415248 6.1:+1575778 None:intergenic
TTCAGTGGCCACGGCGGAGA+TGG 0.416551 6.1:+1575841 None:intergenic
GCGGCGACAGTGGCGGCGGC+GGG 0.421106 6.1:+1575713 None:intergenic
GGCGGCGGGAGCGAAGGAAG+CGG 0.431011 6.1:+1575727 None:intergenic
AGTGCGGTGGTGGTGGGAGA+TGG 0.438739 6.1:+1575874 None:intergenic
GTGGTGGTGGGAGATGGAGA+GGG 0.450215 6.1:+1575880 None:intergenic
TGGAGCGGCGACAGTGGCGG+CGG 0.453386 6.1:+1575709 None:intergenic
AGTCGACGAGGTCTTTGGCT+TGG 0.457705 6.1:+1575777 None:intergenic
GAAGCGAAGGAATTGATTGA+AGG 0.470442 6.1:-1575563 MS.gene82878:CDS
GGCTGGAGCGGCGACAGTGG+CGG 0.474590 6.1:+1575706 None:intergenic
TTTGGAGGGGAGATTGAGAG+TGG 0.477307 6.1:+1575904 None:intergenic
ACTACTGCGACTTCGGCGGC+TGG 0.482100 6.1:+1575689 None:intergenic
GAGATGGAGAGGGGTTTGGA+GGG 0.482993 6.1:+1575890 None:intergenic
GACGGAGACGAGTGCGGTGG+TGG 0.484373 6.1:+1575864 None:intergenic
TGCGACTTCGGCGGCTGGAG+CGG 0.489099 6.1:+1575694 None:intergenic
AGTGGCGGCGGCGGGAGCGA+AGG 0.497332 6.1:+1575721 None:intergenic
TTGCCGAAGAAATTTAAAGA+AGG 0.505106 6.1:-1575539 MS.gene82878:CDS
TCAGTGGCCACGGCGGAGAT+GGG 0.542642 6.1:+1575842 None:intergenic
TCCTAGTAACGCGAGAATTG+CGG 0.544335 6.1:-1575627 MS.gene82878:CDS
GAGACGAGTGCGGTGGTGGT+GGG 0.552373 6.1:+1575868 None:intergenic
GTGGTGAGAAAGTGTGAGGG+TGG 0.553869 6.1:+1575952 None:intergenic
GGCGGCTGGAGCGGCGACAG+TGG 0.556006 6.1:+1575703 None:intergenic
GGCTTGGGAGAGAGTGAGGT+CGG 0.562669 6.1:+1575793 None:intergenic
AAGAAACAACTTGAAGCTGC+AGG 0.564071 6.1:-1575488 MS.gene82878:CDS
TGGCCACGGCGGAGATGGGA+CGG 0.564429 6.1:+1575846 None:intergenic
CTTTGGCTTGGGAGAGAGTG+AGG 0.572512 6.1:+1575789 None:intergenic
GGCGGGAGCGAAGGAAGCGG+CGG 0.574913 6.1:+1575730 None:intergenic
GGTGTGGTGAGAAAGTGTGA+GGG 0.597396 6.1:+1575949 None:intergenic
GGGTGTGGTGAGAAAGTGTG+AGG 0.603340 6.1:+1575948 None:intergenic
TTTGGGATTGAAAGAAGCGA+AGG 0.613094 6.1:-1575576 MS.gene82878:CDS
TTCGACTACTGCGACTTCGG+CGG 0.637460 6.1:+1575685 None:intergenic
GAGAATTGCGGCGATTAAAG+CGG 0.638455 6.1:-1575615 MS.gene82878:CDS
TTCTATTTCAGTGGCCACGG+CGG 0.638885 6.1:+1575835 None:intergenic
GGTGGAGATAGTTGTGAAAG+CGG 0.640687 6.1:+1575970 None:intergenic
TCTCCGTCCCATCTCCGCCG+TGG 0.646628 6.1:-1575849 MS.gene82878:CDS
AGATGGGACGGAGACGAGTG+CGG 0.648615 6.1:+1575858 None:intergenic
TTTCTTGGAGGTAGTCGACG+AGG 0.676894 6.1:+1575765 None:intergenic
TGGGACGGAGACGAGTGCGG+TGG 0.682439 6.1:+1575861 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACACCTTCTTTAAATTTCTT+CGG + chr6.1:1575919-1575938 None:intergenic 25.0%
TTCAATTACCACATCGAATT+CGG + chr6.1:1575800-1575819 None:intergenic 30.0%
TTGCCGAAGAAATTTAAAGA+AGG - chr6.1:1575913-1575932 MS.gene82878:CDS 30.0%
!!! AGATTGATTTTTTTGAGAGG+GGG + chr6.1:1575528-1575547 None:intergenic 30.0%
!!! GAGATTGATTTTTTTGAGAG+GGG + chr6.1:1575529-1575548 None:intergenic 30.0%
!!! GGAGATTGATTTTTTTGAGA+GGG + chr6.1:1575530-1575549 None:intergenic 30.0%
!!! TGGAGATTGATTTTTTTGAG+AGG + chr6.1:1575531-1575550 None:intergenic 30.0%
AGAAAAAACCGAATTCGATG+TGG - chr6.1:1575789-1575808 MS.gene82878:CDS 35.0%
GCCACTGAAATAGAAAAACT+CGG - chr6.1:1575625-1575644 MS.gene82878:CDS 35.0%
! GTTAGAGCTTTAACGAGTTT+GGG - chr6.1:1575859-1575878 MS.gene82878:CDS 35.0%
! TTTTTCTATTTCAGTGGCCA+CGG + chr6.1:1575623-1575642 None:intergenic 35.0%
!!! GATTGATTTTTTTGAGAGGG+GGG + chr6.1:1575527-1575546 None:intergenic 35.0%
AAGAAACAACTTGAAGCTGC+AGG - chr6.1:1575964-1575983 MS.gene82878:CDS 40.0%
GAAGCGAAGGAATTGATTGA+AGG - chr6.1:1575889-1575908 MS.gene82878:CDS 40.0%
GACTACCTCCAAGAAAAACT+CGG - chr6.1:1575694-1575713 MS.gene82878:CDS 40.0%
! GCCGAGTTTTTCTATTTCAG+TGG + chr6.1:1575629-1575648 None:intergenic 40.0%
! GGTTAGAGCTTTAACGAGTT+TGG - chr6.1:1575858-1575877 MS.gene82878:CDS 40.0%
! TTTGGGATTGAAAGAAGCGA+AGG - chr6.1:1575876-1575895 MS.gene82878:CDS 40.0%
GAGAATTGCGGCGATTAAAG+CGG - chr6.1:1575837-1575856 MS.gene82878:CDS 45.0%
GGTGGAGATAGTTGTGAAAG+CGG + chr6.1:1575485-1575504 None:intergenic 45.0%
TCCTAGTAACGCGAGAATTG+CGG - chr6.1:1575825-1575844 MS.gene82878:CDS 45.0%
TTCTTCGACTACTGCGACTT+CGG + chr6.1:1575773-1575792 None:intergenic 45.0%
!!! ATTTTTTTGAGAGGGGGGTG+TGG + chr6.1:1575522-1575541 None:intergenic 45.0%
TTCTATTTCAGTGGCCACGG+CGG + chr6.1:1575620-1575639 None:intergenic 50.0%
TTTCTTGGAGGTAGTCGACG+AGG + chr6.1:1575690-1575709 None:intergenic 50.0%
TTTGGAGGGGAGATTGAGAG+TGG + chr6.1:1575551-1575570 None:intergenic 50.0%
! GGTGTGGTGAGAAAGTGTGA+GGG + chr6.1:1575506-1575525 None:intergenic 50.0%
! TGACGCCGAGTTTTTCTTGG+AGG + chr6.1:1575702-1575721 None:intergenic 50.0%
AGATGGAGAGGGGTTTGGAG+GGG + chr6.1:1575564-1575583 None:intergenic 55.0%
AGTCGACGAGGTCTTTGGCT+TGG + chr6.1:1575678-1575697 None:intergenic 55.0%
GAGATGGAGAGGGGTTTGGA+GGG + chr6.1:1575565-1575584 None:intergenic 55.0%
GAGGTAGTCGACGAGGTCTT+TGG + chr6.1:1575683-1575702 None:intergenic 55.0%
GCCGCAATTCTCGCGTTACT+AGG + chr6.1:1575829-1575848 None:intergenic 55.0%
TTCGACTACTGCGACTTCGG+CGG + chr6.1:1575770-1575789 None:intergenic 55.0%
! CGGTGACGCCGAGTTTTTCT+TGG + chr6.1:1575705-1575724 None:intergenic 55.0%
! GGGTGTGGTGAGAAAGTGTG+AGG + chr6.1:1575507-1575526 None:intergenic 55.0%
! GTCGACGAGGTCTTTGGCTT+GGG + chr6.1:1575677-1575696 None:intergenic 55.0%
! GTGGTGAGAAAGTGTGAGGG+TGG + chr6.1:1575503-1575522 None:intergenic 55.0%
!! CTTTGGCTTGGGAGAGAGTG+AGG + chr6.1:1575666-1575685 None:intergenic 55.0%
AGATGGGACGGAGACGAGTG+CGG + chr6.1:1575597-1575616 None:intergenic 60.0%
GGAGATGGAGAGGGGTTTGG+AGG + chr6.1:1575566-1575585 None:intergenic 60.0%
GGCTTGGGAGAGAGTGAGGT+CGG + chr6.1:1575662-1575681 None:intergenic 60.0%
GTGGGAGATGGAGAGGGGTT+TGG + chr6.1:1575569-1575588 None:intergenic 60.0%
!! GTGGTGGTGGGAGATGGAGA+GGG + chr6.1:1575575-1575594 None:intergenic 60.0%
!! TGGTGGTGGGAGATGGAGAG+GGG + chr6.1:1575574-1575593 None:intergenic 60.0%
ACTACTGCGACTTCGGCGGC+TGG + chr6.1:1575766-1575785 None:intergenic 65.0%
TCAGTGGCCACGGCGGAGAT+GGG + chr6.1:1575613-1575632 None:intergenic 65.0%
TTCAGTGGCCACGGCGGAGA+TGG + chr6.1:1575614-1575633 None:intergenic 65.0%
! GAGACGAGTGCGGTGGTGGT+GGG + chr6.1:1575587-1575606 None:intergenic 65.0%
!! AGTGCGGTGGTGGTGGGAGA+TGG + chr6.1:1575581-1575600 None:intergenic 65.0%
!! GGTGGTGGTGGGAGATGGAG+AGG + chr6.1:1575576-1575595 None:intergenic 65.0%
GACGGAGACGAGTGCGGTGG+TGG + chr6.1:1575591-1575610 None:intergenic 70.0%
TCTCCGTCCCATCTCCGCCG+TGG - chr6.1:1575603-1575622 MS.gene82878:CDS 70.0%
TGCGACTTCGGCGGCTGGAG+CGG + chr6.1:1575761-1575780 None:intergenic 70.0%
TGGCCACGGCGGAGATGGGA+CGG + chr6.1:1575609-1575628 None:intergenic 70.0%
TGGGACGGAGACGAGTGCGG+TGG + chr6.1:1575594-1575613 None:intergenic 70.0%
! GGAGACGAGTGCGGTGGTGG+TGG + chr6.1:1575588-1575607 None:intergenic 70.0%
GGCGGCGGGAGCGAAGGAAG+CGG + chr6.1:1575728-1575747 None:intergenic 75.0%
GGCGGGAGCGAAGGAAGCGG+CGG + chr6.1:1575725-1575744 None:intergenic 75.0%
GGCTGGAGCGGCGACAGTGG+CGG + chr6.1:1575749-1575768 None:intergenic 75.0%
TGGAGCGGCGACAGTGGCGG+CGG + chr6.1:1575746-1575765 None:intergenic 75.0%
! AGCGGCGACAGTGGCGGCGG+CGG + chr6.1:1575743-1575762 None:intergenic 80.0%
! AGTGGCGGCGGCGGGAGCGA+AGG + chr6.1:1575734-1575753 None:intergenic 80.0%
! GGCGGCTGGAGCGGCGACAG+TGG + chr6.1:1575752-1575771 None:intergenic 80.0%
! GCGGCGACAGTGGCGGCGGC+GGG + chr6.1:1575742-1575761 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 1575466 1576008 1575466 ID=MS.gene82878
chr6.1 mRNA 1575466 1576008 1575466 ID=MS.gene82878.t1;Parent=MS.gene82878
chr6.1 exon 1575466 1576008 1575466 ID=MS.gene82878.t1.exon1;Parent=MS.gene82878.t1
chr6.1 CDS 1575466 1576008 1575466 ID=cds.MS.gene82878.t1;Parent=MS.gene82878.t1
Gene Sequence

>MS.gene82878

ATGGCATTATCAACAACCGCTTTCACAACTATCTCCACCCTCACACTTTCTCACCACACCCCCCTCTCAAAAAAATCAATCTCCACTCTCAATCTCCCCTCCAAACCCCTCTCCATCTCCCACCACCACCGCACTCGTCTCCGTCCCATCTCCGCCGTGGCCACTGAAATAGAAAAACTCGGCAACGACATCTCCGACCTCACTCTCTCCCAAGCCAAAGACCTCGTCGACTACCTCCAAGAAAAACTCGGCGTCACCGCCGCTTCCTTCGCTCCCGCCGCCGCCACTGTCGCCGCTCCAGCCGCCGAAGTCGCAGTAGTCGAAGAAAAAACCGAATTCGATGTGGTAATTGAAGATGTTCCTAGTAACGCGAGAATTGCGGCGATTAAAGCGGTTAGAGCTTTAACGAGTTTGGGATTGAAAGAAGCGAAGGAATTGATTGAAGGTTTGCCGAAGAAATTTAAAGAAGGTGTGAGTAAAGATGAAGCTGATGATGCTAAGAAACAACTTGAAGCTGCAGGTGCTAAAGTTACTATTGCTTAA

Protein sequence

>MS.gene82878.t1

MALSTTAFTTISTLTLSHHTPLSKKSISTLNLPSKPLSISHHHRTRLRPISAVATEIEKLGNDISDLTLSQAKDLVDYLQEKLGVTAASFAPAAATVAAPAAEVAVVEEKTEFDVVIEDVPSNARIAAIKAVRALTSLGLKEAKELIEGLPKKFKEGVSKDEADDAKKQLEAAGAKVTIA