AlfalfaGEDB | Msa0706380

M. sativa cultivar zhongmu-4 / Msa0706380

KEGG: -
GO: -
PFAM: PF00650.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0706380	XP_013446941.1	99.324	148	1	0	1	148	49	196	3.73e-103	305

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0706380	A0A072TVS7	99.324	148	1	0	1	148	49	196	1.78e-103	305

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003470	Msa0706380	0.835082	2.064190e-56	-8.615850e-47
Msa0004350	Msa0706380	0.824920	6.214683e-54	-8.615850e-47
Msa0006700	Msa0706380	0.826721	2.322100e-54	-8.615850e-47
Msa0008850	Msa0706380	0.861587	9.236373e-64	-8.615850e-47
Msa0017120	Msa0706380	0.848995	4.293649e-60	-8.615850e-47
Msa0020630	Msa0706380	0.855055	8.151714e-62	-8.615850e-47
Msa0024680	Msa0706380	0.806036	9.998452e-50	-8.615850e-47
Msa0027540	Msa0706380	0.847549	1.078041e-59	-8.615850e-47
Msa0030000	Msa0706380	0.846028	2.808673e-59	-8.615850e-47
Msa0030590	Msa0706380	0.834281	3.282159e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0706380	MtrunA17_Chr8g0385691	99.324	148	1	0	1	148	49	196	3.43e-107	305
Msa0706380	MtrunA17_Chr8g0385681	91.156	147	13	0	1	147	52	198	6.92e-98	282
Msa0706380	MtrunA17_Chr3g0131581	70.068	147	44	0	1	147	69	215	2.35e-78	233
Msa0706380	MtrunA17_Chr1g0161441	44.667	150	77	3	2	146	32	180	3.65e-37	127
Msa0706380	MtrunA17_Chr4g0058811	30.405	148	101	2	1	147	73	219	1.51e-21	87.4
Msa0706380	MtrunA17_Chr4g0058791	29.054	148	103	2	1	147	71	217	2.06e-21	86.7
Msa0706380	MtrunA17_Chr1g0155071	32.215	149	96	4	2	148	46	191	2.98e-21	87.0
Msa0706380	MtrunA17_Chr3g0115591	30.612	147	98	4	2	147	52	195	3.82e-17	76.3
Msa0706380	MtrunA17_Chr5g0425421	30.345	145	96	4	2	145	49	189	1.12e-14	69.7
Msa0706380	MtrunA17_Chr1g0205531	26.351	148	107	2	2	148	42	188	6.89e-14	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0706380	AT1G01630.1	62.585	147	55	0	1	147	52	198	5.22e-68	206
Msa0706380	AT1G14820.2	44.295	149	77	3	2	145	18	165	2.12e-32	115
Msa0706380	AT1G14820.1	44.295	149	77	3	2	145	18	165	2.12e-32	115
Msa0706380	AT1G14820.3	44.000	150	78	3	1	145	30	178	2.34e-32	115
Msa0706380	AT4G36640.1	33.557	149	94	4	2	148	44	189	3.08e-19	82.0
Msa0706380	AT4G36640.3	33.557	149	94	4	2	148	44	189	3.08e-19	82.0
Msa0706380	AT5G63060.2	30.872	149	101	2	1	148	39	186	3.47e-19	80.9
Msa0706380	AT5G63060.1	32.450	151	96	3	1	148	74	221	8.03e-19	80.5
Msa0706380	AT1G75170.2	30.201	149	99	4	2	148	47	192	2.59e-18	79.7
Msa0706380	AT1G75170.1	30.201	149	99	4	2	148	47	192	2.59e-18	79.7

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Find 65 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACTTCCCCAATCTTTC+AGG	0.132673	4_4:-79348769	None:intergenic
ACAACTTCCCCAATCTTTCA+GGG	0.493166	4_4:-79348768	None:intergenic
ACCAATCTTGTCATGTCCTT+GGG	0.434729	4_4:-79347777	None:intergenic
ACTGCAATTAGTCCCCATCC+AGG	0.559870	4_4:+79349067	Msa0706380:three_prime_UTR
ACTTGTTACAATGAGTTTCT+TGG	0.323539	4_4:+79349371	Msa0706380:three_prime_UTR
AGACATTCATTTGTTCCAAA+TGG	0.425215	4_4:+79347701	Msa0706380:CDS
AGCGATTTGCGTGGATACAT+TGG	0.523132	4_4:+79348625	Msa0706380:CDS
AGGAACCTGTGATAGAGATA+TGG	0.459166	4_4:-79347726	None:intergenic
AGTCAACTTCCGGAGATATA+CGG	0.395789	4_4:+79349038	Msa0706380:three_prime_UTR
AGTTTAATAGCTATCCTGGA+TGG	0.519602	4_4:-79349081	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGACATTCATTTGTTCCAAA+TGG	+	chr4_4:79347701-79347720	Msa0706380:CDS	30.0%
TTGTCTTGTGCAATTTGATT+AGG	-	chr4_4:79347749-79347768	None:intergenic	30.0%
TAATCAAATTGCACAAGACA+AGG	+	chr4_4:79347748-79347767	Msa0706380:CDS	30.0%
TGTTAACTAGATATAGGGAT+AGG	-	chr4_4:79347912-79347931	None:intergenic	30.0%
GATCTCTATTTGAGTATTAG+AGG	+	chr4_4:79348140-79348159	Msa0706380:intron	30.0%
TTCTTGATAAACTATGTGCA+AGG	+	chr4_4:79348457-79348476	Msa0706380:CDS	30.0%
ATAACGATATACCTGCAAAA+TGG	-	chr4_4:79348659-79348678	None:intergenic	30.0%
ATTGTTAATTTCTTGGATGC+AGG	+	chr4_4:79348738-79348757	Msa0706380:intron	30.0%
CCACACTTTCATAAAATGTA+CGG	-	chr4_4:79348808-79348827	None:intergenic	30.0%
TGACAACAAAACTAAGAAGA+AGG	+	chr4_4:79348848-79348867	Msa0706380:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	79347606	79349641	79347606	ID=Msa0706380;Name=Msa0706380
chr4_4	mRNA	79347606	79349641	79347606	ID=Msa0706380-mRNA-1;Parent=Msa0706380;Name=Msa0706380-mRNA-1;_AED=0.09;_eAED=0.09;_QI=17\|1\|1\|1\|0.75\|0.6\|5\|711\|148
chr4_4	exon	79347606	79347866	79347606	ID=Msa0706380-mRNA-1:exon:25495;Parent=Msa0706380-mRNA-1
chr4_4	exon	79348442	79348478	79348442	ID=Msa0706380-mRNA-1:exon:25496;Parent=Msa0706380-mRNA-1
chr4_4	exon	79348561	79348666	79348561	ID=Msa0706380-mRNA-1:exon:25497;Parent=Msa0706380-mRNA-1
chr4_4	exon	79348760	79348869	79348760	ID=Msa0706380-mRNA-1:exon:25498;Parent=Msa0706380-mRNA-1
chr4_4	exon	79348981	79349641	79348981	ID=Msa0706380-mRNA-1:exon:25499;Parent=Msa0706380-mRNA-1
chr4_4	five_prime_UTR	79347606	79347622	79347606	ID=Msa0706380-mRNA-1:five_prime_utr;Parent=Msa0706380-mRNA-1
chr4_4	CDS	79347623	79347866	79347623	ID=Msa0706380-mRNA-1:cds;Parent=Msa0706380-mRNA-1
chr4_4	CDS	79348442	79348478	79348442	ID=Msa0706380-mRNA-1:cds;Parent=Msa0706380-mRNA-1
chr4_4	CDS	79348561	79348666	79348561	ID=Msa0706380-mRNA-1:cds;Parent=Msa0706380-mRNA-1
chr4_4	CDS	79348760	79348819	79348760	ID=Msa0706380-mRNA-1:cds;Parent=Msa0706380-mRNA-1
chr4_4	three_prime_UTR	79348820	79348869	79348820	ID=Msa0706380-mRNA-1:three_prime_utr;Parent=Msa0706380-mRNA-1
chr4_4	three_prime_UTR	79348981	79349641	79348981	ID=Msa0706380-mRNA-1:three_prime_utr;Parent=Msa0706380-mRNA-1

Gene Sequence

>Msa0706380

ATGATCAGAAGATTTCTACGTGCTCGTGATTTAGATGTAGAGAAGGCTTCAGCAATGTTCCTCAAGTACTTGAAATGGAGACATTCATTTGTTCCAAATGGTTCCATATCTCTATCACAGGTTCCTAATCAAATTGCACAAGACAAGGCCTTCGCCCAAGGACATGACAAGATTGGTCGACCAATACTTCTTGTCTTTGGTGGAAGACATTTTCAAAAGAAGGATGGTCTAGATGAATTCAAACGGTTTGCAGTTTATATTCTTGATAAACTATGTGCAAGCATGCCACCTGGGGAAGAAAAATTTGTTGGTATAGCAGAACTCAAAGGATGGGGTTACTCAAATAGCGATTTGCGTGGATACATTGGTGCCCTCTCCATTTTGCAGGATTACTACCCTGAAAGATTGGGGAAGTTGTTTATTCTCCACGCACCGTACATTTTATGA

Protein sequence

>Msa0706380

MIRRFLRARDLDVEKASAMFLKYLKWRHSFVPNGSISLSQVPNQIAQDKAFAQGHDKIGRPILLVFGGRHFQKKDGLDEFKRFAVYILDKLCASMPPGEEKFVGIAELKGWGYSNSDLRGYIGALSILQDYYPERLGKLFILHAPYIL*