AlfalfaGEDB | Msa0843120

M. sativa cultivar zhongmu-4 / Msa0843120

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0843120	KEH27764.1	56.122	196	73	7	3	186	7	201	4.50e-57	188

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0843120	A0A072UD94	56.122	196	73	7	3	186	7	201	2.15e-57	188

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004540	Msa0843120	-0.812246	4.668397e-51	-8.615850e-47
Msa0010770	Msa0843120	-0.811224	7.792058e-51	-8.615850e-47
Msa0022750	Msa0843120	0.851854	6.766565e-61	-8.615850e-47
Msa0039250	Msa0843120	-0.819670	1.028219e-52	-8.615850e-47
Msa0047250	Msa0843120	-0.813644	2.305468e-51	-8.615850e-47
Msa0054820	Msa0843120	-0.813085	3.059296e-51	-8.615850e-47
Msa0055070	Msa0843120	-0.825726	4.004859e-54	-8.615850e-47
Msa0057590	Msa0843120	-0.808465	3.056212e-50	-8.615850e-47
Msa0070170	Msa0843120	-0.824658	7.163797e-54	-8.615850e-47
Msa0070430	Msa0843120	-0.837320	5.570877e-57	-8.615850e-47
Msa0074750	Msa0843120	-0.811880	5.611007e-51	-8.615850e-47
Msa0096580	Msa0843120	-0.803555	3.296716e-49	-8.615850e-47
Msa0097950	Msa0843120	-0.811767	5.939038e-51	-8.615850e-47
Msa0100160	Msa0843120	-0.833488	5.180640e-56	-8.615850e-47
Msa0101020	Msa0843120	-0.819287	1.257288e-52	-8.615850e-47
Msa0103250	Msa0843120	-0.800841	1.192593e-48	-8.615850e-47
Msa0115370	Msa0843120	-0.804526	2.070820e-49	-8.615850e-47
Msa0606810	Msa0843120	-0.804487	2.109749e-49	-8.615850e-47
Msa0607520	Msa0843120	-0.810253	1.263790e-50	-8.615850e-47
Msa0614510	Msa0843120	-0.806780	6.964680e-50	-8.615850e-47
Msa0617770	Msa0843120	0.800487	1.408517e-48	-8.615850e-47
Msa0618950	Msa0843120	-0.828247	9.998483e-55	-8.615850e-47
Msa0618970	Msa0843120	-0.820263	7.523398e-53	-8.615850e-47
Msa0619920	Msa0843120	-0.804930	1.705028e-49	-8.615850e-47
Msa0620230	Msa0843120	-0.807979	3.878498e-50	-8.615850e-47
Msa0620330	Msa0843120	-0.804595	2.003057e-49	-8.615850e-47
Msa0628420	Msa0843120	-0.835432	1.684064e-56	-8.615850e-47
Msa0628500	Msa0843120	-0.804358	2.244183e-49	-8.615850e-47
Msa0652690	Msa0843120	-0.843448	1.392394e-58	-8.615850e-47
Msa0659990	Msa0843120	-0.820085	8.262092e-53	-8.615850e-47
Msa0662630	Msa0843120	-0.814252	1.693552e-51	-8.615850e-47
Msa0667790	Msa0843120	-0.800672	1.291268e-48	-8.615850e-47
Msa0675990	Msa0843120	-0.830215	3.329666e-55	-8.615850e-47
Msa0686080	Msa0843120	-0.807507	4.886783e-50	-8.615850e-47
Msa0687230	Msa0843120	-0.821788	3.351219e-53	-8.615850e-47
Msa0697720	Msa0843120	-0.819665	1.031013e-52	-8.615850e-47
Msa0703440	Msa0843120	-0.819991	8.683762e-53	-8.615850e-47
Msa0705390	Msa0843120	-0.833865	4.171654e-56	-8.615850e-47
Msa0710270	Msa0843120	-0.816471	5.435718e-52	-8.615850e-47
Msa0716410	Msa0843120	-0.837704	4.442702e-57	-8.615850e-47
Msa0726080	Msa0843120	-0.835696	1.443601e-56	-8.615850e-47
Msa0734960	Msa0843120	-0.808297	3.319496e-50	-8.615850e-47
Msa0744090	Msa0843120	-0.811877	5.618098e-51	-8.615850e-47
Msa0744100	Msa0843120	-0.814705	1.344690e-51	-8.615850e-47
Msa0745200	Msa0843120	-0.803389	3.568958e-49	-8.615850e-47
Msa0788110	Msa0843120	-0.816448	5.501546e-52	-8.615850e-47
Msa0788120	Msa0843120	-0.827520	1.495257e-54	-8.615850e-47
Msa0811130	Msa0843120	-0.807628	4.606383e-50	-8.615850e-47
Msa0819540	Msa0843120	-0.822820	1.930951e-53	-8.615850e-47
Msa0825270	Msa0843120	-0.810996	8.731044e-51	-8.615850e-47
Msa0825280	Msa0843120	-0.819066	1.411263e-52	-8.615850e-47
Msa0836500	Msa0843120	-0.827794	1.284626e-54	-8.615850e-47
Msa0127850	Msa0843120	-0.812706	3.704065e-51	-8.615850e-47
Msa0130240	Msa0843120	-0.818215	2.202216e-52	-8.615850e-47
Msa0133720	Msa0843120	-0.807871	4.089719e-50	-8.615850e-47
Msa0136120	Msa0843120	-0.816046	6.765697e-52	-8.615850e-47
Msa0142500	Msa0843120	-0.807960	3.915702e-50	-8.615850e-47
Msa0145110	Msa0843120	-0.819456	1.150722e-52	-8.615850e-47
Msa0146080	Msa0843120	-0.829504	4.962240e-55	-8.615850e-47
Msa0147980	Msa0843120	-0.815502	8.945615e-52	-8.615850e-47
Msa0160670	Msa0843120	-0.821252	4.457619e-53	-8.615850e-47
Msa0164370	Msa0843120	-0.805190	1.504441e-49	-8.615850e-47
Msa0208810	Msa0843120	-0.802059	6.714889e-49	-8.615850e-47
Msa0210260	Msa0843120	-0.808920	2.442955e-50	-8.615850e-47
Msa0237200	Msa0843120	-0.800736	1.252886e-48	-8.615850e-47
Msa0378970	Msa0843120	-0.815971	7.033694e-52	-8.615850e-47
Msa0384670	Msa0843120	-0.818209	2.209046e-52	-8.615850e-47
Msa0387880	Msa0843120	0.819855	9.328577e-53	-8.615850e-47
Msa0392200	Msa0843120	-0.847145	1.391840e-59	-8.615850e-47
Msa0394690	Msa0843120	-0.802261	6.099912e-49	-8.615850e-47
Msa0421300	Msa0843120	0.810202	1.295800e-50	-8.615850e-47
Msa0428050	Msa0843120	-0.808497	3.009050e-50	-8.615850e-47
Msa0431770	Msa0843120	-0.806816	6.844357e-50	-8.615850e-47
Msa0434570	Msa0843120	-0.809477	1.856125e-50	-8.615850e-47
Msa0434770	Msa0843120	-0.806458	8.146364e-50	-8.615850e-47
Msa0439890	Msa0843120	-0.801105	1.053248e-48	-8.615850e-47
Msa0443430	Msa0843120	-0.813872	2.053782e-51	-8.615850e-47
Msa0461590	Msa0843120	-0.823261	1.523213e-53	-8.615850e-47
Msa0467260	Msa0843120	0.833415	5.402625e-56	-8.615850e-47
Msa0474940	Msa0843120	-0.807918	3.997542e-50	-8.615850e-47
Msa0480780	Msa0843120	-0.843890	1.060527e-58	-8.615850e-47
Msa0244980	Msa0843120	-0.808826	2.559300e-50	-8.615850e-47
Msa0267870	Msa0843120	-0.822260	2.604746e-53	-8.615850e-47
Msa0303460	Msa0843120	-0.803497	3.389175e-49	-8.615850e-47
Msa0319330	Msa0843120	-0.803302	3.719988e-49	-8.615850e-47
Msa0322590	Msa0843120	-0.811182	7.955480e-51	-8.615850e-47
Msa0322900	Msa0843120	-0.801672	8.062109e-49	-8.615850e-47
Msa0341690	Msa0843120	-0.814731	1.326672e-51	-8.615850e-47
Msa0343190	Msa0843120	-0.810497	1.119290e-50	-8.615850e-47
Msa0346990	Msa0843120	-0.840858	6.747888e-58	-8.615850e-47
Msa0347990	Msa0843120	-0.812272	4.609406e-51	-8.615850e-47
Msa0484740	Msa0843120	-0.806885	6.619214e-50	-8.615850e-47
Msa0485240	Msa0843120	-0.803830	2.891332e-49	-8.615850e-47
Msa0487780	Msa0843120	-0.809217	2.110287e-50	-8.615850e-47
Msa0514610	Msa0843120	-0.803954	2.723895e-49	-8.615850e-47
Msa0517090	Msa0843120	-0.805325	1.409560e-49	-8.615850e-47
Msa0518440	Msa0843120	-0.807841	4.150794e-50	-8.615850e-47
Msa0531250	Msa0843120	-0.826836	2.179982e-54	-8.615850e-47
Msa0532980	Msa0843120	-0.816663	4.923136e-52	-8.615850e-47
Msa0547990	Msa0843120	-0.813095	3.043767e-51	-8.615850e-47
Msa0560500	Msa0843120	-0.807501	4.901841e-50	-8.615850e-47
Msa0576680	Msa0843120	-0.811521	6.716175e-51	-8.615850e-47
Msa0585070	Msa0843120	-0.805452	1.326089e-49	-8.615850e-47
Msa0588090	Msa0843120	-0.828122	1.071472e-54	-8.615850e-47
Msa0588120	Msa0843120	-0.816156	6.394931e-52	-8.615850e-47
Msa0843120	Msa0846230	-0.832297	1.024430e-55	-8.615850e-47
Msa0843120	Msa0849440	-0.833626	4.786557e-56	-8.615850e-47
Msa0843120	Msa0851470	-0.823166	1.603346e-53	-8.615850e-47
Msa0843120	Msa0853950	-0.820723	5.900776e-53	-8.615850e-47
Msa0843120	Msa0864420	-0.802869	4.572426e-49	-8.615850e-47
Msa0843120	Msa0880450	-0.812003	5.274157e-51	-8.615850e-47
Msa0843120	Msa0903780	-0.812966	3.248342e-51	-8.615850e-47
Msa0843120	Msa0905750	-0.804768	1.843038e-49	-8.615850e-47
Msa0843120	Msa0926670	-0.800844	1.190986e-48	-8.615850e-47
Msa0843120	Msa0939000	-0.806986	6.302278e-50	-8.615850e-47
Msa0843120	Msa0943170	-0.809826	1.561841e-50	-8.615850e-47
Msa0843120	Msa0945240	-0.845179	4.772300e-59	-8.615850e-47
Msa0843120	Msa0947530	0.804241	2.373850e-49	-8.615850e-47
Msa0843120	Msa0957870	-0.821222	4.529469e-53	-8.615850e-47
Msa0843120	Msa0957970	-0.823253	1.530051e-53	-8.615850e-47
Msa0843120	Msa0980540	-0.806646	7.436436e-50	-8.615850e-47
Msa0843120	Msa0980940	-0.827942	1.183966e-54	-8.615850e-47
Msa0843120	Msa1004420	-0.809878	1.521700e-50	-8.615850e-47
Msa0843120	Msa1025260	-0.801966	7.017720e-49	-8.615850e-47
Msa0843120	Msa1025940	-0.814340	1.619073e-51	-8.615850e-47
Msa0843120	Msa1040840	-0.801293	9.641316e-49	-8.615850e-47
Msa0843120	Msa1050910	-0.819714	1.004725e-52	-8.615850e-47
Msa0843120	Msa1051600	-0.825016	5.897944e-54	-8.615850e-47
Msa0843120	Msa1070390	-0.822600	2.171548e-53	-8.615850e-47
Msa0843120	Msa1080960	-0.800076	1.707373e-48	-8.615850e-47
Msa0843120	Msa1087510	0.804103	2.536800e-49	-8.615850e-47
Msa0843120	Msa1088250	0.831424	1.682355e-55	-8.615850e-47
Msa0843120	Msa1109350	-0.806855	6.717330e-50	-8.615850e-47
Msa0843120	Msa1113590	-0.809951	1.467707e-50	-8.615850e-47
Msa0843120	Msa1123350	-0.829311	5.526925e-55	-8.615850e-47
Msa0843120	Msa1135810	0.820919	5.317707e-53	-8.615850e-47
Msa0843120	Msa1154490	-0.814649	1.383231e-51	-8.615850e-47
Msa0843120	Msa1158230	-0.818705	1.705078e-52	-8.615850e-47
Msa0843120	Msa1169720	-0.807231	5.591193e-50	-8.615850e-47
Msa0843120	Msa1171830	-0.828220	1.014635e-54	-8.615850e-47
Msa0843120	Msa1206650	-0.825220	5.278258e-54	-8.615850e-47
Msa0843120	Msa1208160	-0.802504	5.437837e-49	-8.615850e-47
Msa0843120	Msa1219000	-0.809926	1.486525e-50	-8.615850e-47
Msa0843120	Msa1227460	-0.815617	8.435143e-52	-8.615850e-47
Msa0843120	Msa1250110	-0.811171	8.001755e-51	-8.615850e-47
Msa0843120	Msa1250730	-0.810454	1.143596e-50	-8.615850e-47
Msa0843120	Msa1270420	-0.817434	3.304161e-52	-8.615850e-47
Msa0843120	Msa1279590	-0.808422	3.121890e-50	-8.615850e-47
Msa0843120	Msa1286550	-0.809952	1.467350e-50	-8.615850e-47
Msa0843120	Msa1297600	-0.818250	2.162111e-52	-8.615850e-47
Msa0843120	Msa1306390	-0.813939	1.985742e-51	-8.615850e-47
Msa0843120	Msa1323690	-0.806489	8.022740e-50	-8.615850e-47
Msa0843120	Msa1329450	-0.809388	1.939425e-50	-8.615850e-47
Msa0843120	Msa1332760	-0.806951	6.410678e-50	-8.615850e-47
Msa0843120	Msa1346750	-0.827383	1.613028e-54	-8.615850e-47
Msa0843120	Msa1356610	-0.803851	2.861874e-49	-8.615850e-47
Msa0843120	Msa1359560	-0.835661	1.473586e-56	-8.615850e-47
Msa0843120	Msa1362420	-0.805996	1.019330e-49	-8.615850e-47
Msa0843120	Msa1372310	-0.809403	1.925554e-50	-8.615850e-47
Msa0843120	Msa1379590	-0.802046	6.755045e-49	-8.615850e-47
Msa0843120	Msa1384870	-0.801745	7.789993e-49	-8.615850e-47
Msa0843120	Msa1400120	-0.802025	6.823855e-49	-8.615850e-47
Msa0843120	Msa1410420	-0.803097	4.101703e-49	-8.615850e-47
Msa0843120	Msa1411950	-0.826791	2.235028e-54	-8.615850e-47
Msa0843120	Msa1418240	-0.800637	1.312832e-48	-8.615850e-47
Msa0843120	Msa1424640	-0.802910	4.482915e-49	-8.615850e-47
Msa0843120	Msa1424780	-0.811655	6.279257e-51	-8.615850e-47
Msa0843120	Msa1432130	-0.801484	8.808995e-49	-8.615850e-47
Msa0843120	Msa1439610	-0.811429	7.033575e-51	-8.615850e-47
Msa0843120	Msa1454530	-0.803106	4.084516e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0843120	MtrunA17_Chr5g0413731	54.737	190	73	7	3	180	7	195	5.39e-55	173

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 75 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCAAAATTTGATCCCTTTA+TGG	0.184033	5_4:+20661540	Msa0843120:three_prime_UTR
TTGAAAATTGTCTTCCATAA+AGG	0.235485	5_4:-20661554	None:intergenic
CTGTTCTCTTTAGTCGGTAT+TGG	0.237662	5_4:+20660130	Msa0843120:intron
AAAGTAAGACACCTCTTTAA+AGG	0.239921	5_4:-20660430	None:intergenic
GCTGTTGTTAATTAGGATTT+TGG	0.244977	5_4:+20660271	Msa0843120:intron
AGACACCTCTTTAAAGGTTT+TGG	0.263027	5_4:-20660424	None:intergenic
TAAATGATACAGGGTCTTAA+AGG	0.283245	5_4:+20660583	Msa0843120:intron
TGAAAATTGTCTTCCATAAA+GGG	0.287681	5_4:-20661553	None:intergenic
AGAGAGGAGACGGCGGGAAC+GGG	0.321896	5_4:-20659946	None:intergenic
ATGTGGAGTTCTTGAGTAAT+TGG	0.328263	5_4:-20660455	None:intergenic
CGCATAATATGTATTAATAC+TGG	0.352000	5_4:+20659707	Msa0843120:five_prime_UTR
GCAACGAACCACCGGGTGAT+TGG	0.360230	5_4:-20660013	None:intergenic
GGTATCTCCTCGTCGGGGTT+GGG	0.360858	5_4:-20659916	None:intergenic
GAGAGAGGAGACGGCGGGAA+CGG	0.368696	5_4:-20659947	None:intergenic
AGGTATCTCCTCGTCGGGGT+TGG	0.393919	5_4:-20659917	None:intergenic
CGTCGGCGCGAGCGATTAAA+CGG	0.399318	5_4:-20659834	None:intergenic
GAGACGGCGGGAACGGGAGA+AGG	0.401858	5_4:-20659940	None:intergenic
CCGCGTCAGCGTTGCGTGGG+TGG	0.402985	5_4:-20659765	None:intergenic
CAGCGTTGCGTGGGTGGGTC+GGG	0.408084	5_4:-20659759	None:intergenic
TTAAGTTATAAACAGCAAAA+AGG	0.408563	5_4:-20661630	None:intergenic
TGGCCGACAACGGCGAGAAC+GGG	0.423702	5_4:+20659806	Msa0843120:CDS
ATATCGCCATTTCCTACTAC+TGG	0.424834	5_4:-20661598	None:intergenic
AGTGAGGGAGAGAGGGAGAG+AGG	0.431615	5_4:-20659962	None:intergenic
GAAGGAGGTATCTCCTCGTC+GGG	0.434027	5_4:-20659922	None:intergenic
GGAGAGAGGGAGAGAGGAGA+CGG	0.446013	5_4:-20659956	None:intergenic
TCGAAATACCATTGCTACTC+AGG	0.447368	5_4:+20660303	Msa0843120:CDS
TCAGCGTTGCGTGGGTGGGT+CGG	0.449866	5_4:-20659760	None:intergenic
AGCAACCAAAACCTTTAAAG+AGG	0.470161	5_4:+20660419	Msa0843120:CDS
GTTTATTCGACACCAGTAGT+AGG	0.471594	5_4:+20661586	Msa0843120:three_prime_UTR
TCGGCGGGAGTGAGGGAGAG+AGG	0.471767	5_4:-20659970	None:intergenic
ATGACGATGCAACGAACCAC+CGG	0.478429	5_4:-20660021	None:intergenic
GAGAGGGAGAGAGGAGACGG+CGG	0.478961	5_4:-20659953	None:intergenic
TATCCCGTTCTCGCCGTTGT+CGG	0.481934	5_4:-20659809	None:intergenic
CGCGTCAGCGTTGCGTGGGT+GGG	0.488637	5_4:-20659764	None:intergenic
CCCGTTGGTTTGCTGAAGAT+AGG	0.490333	5_4:-20660617	None:intergenic
TCTATTTCGTCGATGTCGGC+GGG	0.498150	5_4:-20659985	None:intergenic
GCAGGAAGTAGAGCAGCATC+AGG	0.498588	5_4:+20661428	Msa0843120:intron
TCGACACCAGTAGTAGGAAA+TGG	0.501294	5_4:+20661592	Msa0843120:three_prime_UTR
CCACCCACGCAACGCTGACG+CGG	0.505260	5_4:+20659765	Msa0843120:five_prime_UTR
AGAGGGAGAGAGGAGACGGC+GGG	0.508591	5_4:-20659952	None:intergenic
GTTCCAAGCAGTGGATCTAC+TGG	0.509185	5_4:+20661471	Msa0843120:CDS
GAGATATCTCGCGCTATCGT+TGG	0.509441	5_4:+20659859	Msa0843120:CDS
GTCTATTTCGTCGATGTCGG+CGG	0.510243	5_4:-20659986	None:intergenic
CCTATCTTCAGCAAACCAAC+GGG	0.513673	5_4:+20660617	Msa0843120:CDS
GTCGATGTCGGCGGGAGTGA+GGG	0.514142	5_4:-20659977	None:intergenic
CGGCGGGAGTGAGGGAGAGA+GGG	0.515528	5_4:-20659969	None:intergenic
TATGTCTATTTCGTCGATGT+CGG	0.521128	5_4:-20659989	None:intergenic
ATGGCCGACAACGGCGAGAA+CGG	0.525315	5_4:+20659805	Msa0843120:CDS
CGTCGATGTCGGCGGGAGTG+AGG	0.526628	5_4:-20659978	None:intergenic
TTGCCGCGTCAGCGTTGCGT+GGG	0.537465	5_4:-20659768	None:intergenic
TGGTTTCCTTTGTGGAGTTG+AGG	0.539607	5_4:+20661517	Msa0843120:three_prime_UTR
ACGGCGGGAACGGGAGAAGG+AGG	0.547882	5_4:-20659937	None:intergenic
TTTGCCGCGTCAGCGTTGCG+TGG	0.549130	5_4:-20659769	None:intergenic
AACCAACGGGTACTTTAGAC+AGG	0.554816	5_4:+20660630	Msa0843120:CDS
ACCTATCTTCAGCAAACCAA+CGG	0.555623	5_4:+20660616	Msa0843120:CDS
GTCGGCGCGAGCGATTAAAC+GGG	0.556136	5_4:-20659833	None:intergenic
TAGTCGGTATTGGATTCGAA+TGG	0.563622	5_4:+20660140	Msa0843120:intron
TGACGATGCAACGAACCACC+GGG	0.565001	5_4:-20660020	None:intergenic
CTCAAGAACTCCACATCTCA+AGG	0.565553	5_4:+20660462	Msa0843120:CDS
AGAAGGAGGTATCTCCTCGT+CGG	0.577015	5_4:-20659923	None:intergenic
AGAGCTGTTGTTCCAAGCAG+TGG	0.579332	5_4:+20661462	Msa0843120:CDS
CGAAGCAGGATGGCCGACAA+CGG	0.583887	5_4:+20659796	Msa0843120:exon
ATACCAGTAGATCCACTGCT+TGG	0.584757	5_4:-20661474	None:intergenic
CATTAAAATCATACATACAA+TGG	0.590433	5_4:-20661657	None:intergenic
AATATGTATTAATACTGGAT+CGG	0.591376	5_4:+20659712	Msa0843120:five_prime_UTR
ACGCGGCAAAGAAACGAAGC+AGG	0.592781	5_4:+20659782	Msa0843120:five_prime_UTR
AGCGCGAGATATCTCGTCGT+CGG	0.596684	5_4:-20659851	None:intergenic
GGCAAAGAAACGAAGCAGGA+TGG	0.610872	5_4:+20659786	Msa0843120:exon
ATTACATACCTGAGTAGCAA+TGG	0.612955	5_4:-20660311	None:intergenic
GACCTGTCTAAAGTACCCGT+TGG	0.631437	5_4:-20660632	None:intergenic
TTTGCACCTCAACTCCACAA+AGG	0.667623	5_4:-20661523	None:intergenic
AATAGACATATCCAATCACC+CGG	0.682942	5_4:+20660002	Msa0843120:CDS
CAGGAAGCCCAACCCCGACG+AGG	0.688650	5_4:+20659909	Msa0843120:CDS
AAGGAGGTATCTCCTCGTCG+GGG	0.722794	5_4:-20659921	None:intergenic
AGACATATCCAATCACCCGG+TGG	0.761992	5_4:+20660005	Msa0843120:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGCAATTATAGATATTTTTT+TGG	-	chr5_4:20661166-20661185	None:intergenic	15.0%
!!	AAAAAATATCTATAATTGCT+TGG	+	chr5_4:20661166-20661185	Msa0843120:intron	15.0%
!!!	AGCAATTATAGATATTTTTT+TGG	-	chr5_4:20661166-20661185	None:intergenic	15.0%
!!	AAAAAATATCTATAATTGCT+TGG	+	chr5_4:20661166-20661185	Msa0843120:intron	15.0%
!!	AATATGTATTAATACTGGAT+CGG	+	chr5_4:20659712-20659731	Msa0843120:five_prime_UTR	20.0%
!!	TAAGATAATAATGAACATTC+AGG	+	chr5_4:20660393-20660412	Msa0843120:intron	20.0%
!!	TTAAAAATAGAGAACAAAGT+AGG	-	chr5_4:20660501-20660520	None:intergenic	20.0%
!!!	AAAACTTATTTTTAGCATAG+TGG	-	chr5_4:20660888-20660907	None:intergenic	20.0%
!!	TAAACATTACTATTTACTGT+CGG	+	chr5_4:20661136-20661155	Msa0843120:intron	20.0%
!!!	AGAATTTTACTAAAGTTTTG+TGG	-	chr5_4:20661311-20661330	None:intergenic	20.0%
!!	CAAAACTTTAGTAAAATTCT+TGG	+	chr5_4:20661311-20661330	Msa0843120:intron	20.0%
!!!	TCCATTTGAAATAAATTGAA+GGG	-	chr5_4:20661398-20661417	None:intergenic	20.0%
!!!	ATCCATTTGAAATAAATTGA+AGG	-	chr5_4:20661399-20661418	None:intergenic	20.0%
!!	TTAAGTTATAAACAGCAAAA+AGG	-	chr5_4:20661633-20661652	None:intergenic	20.0%
!!!	TTTTTGCTGTTTATAACTTA+AGG	+	chr5_4:20661632-20661651	Msa0843120:three_prime_UTR	20.0%
!!!	TTTTGCTGTTTATAACTTAA+GGG	+	chr5_4:20661633-20661652	Msa0843120:three_prime_UTR	20.0%
!!	CATTAAAATCATACATACAA+TGG	-	chr5_4:20661660-20661679	None:intergenic	20.0%
!!	AATATGTATTAATACTGGAT+CGG	+	chr5_4:20659712-20659731	Msa0843120:five_prime_UTR	20.0%
!!	TAAGATAATAATGAACATTC+AGG	+	chr5_4:20660393-20660412	Msa0843120:intron	20.0%
!!	TTAAAAATAGAGAACAAAGT+AGG	-	chr5_4:20660501-20660520	None:intergenic	20.0%
!!!	AAAACTTATTTTTAGCATAG+TGG	-	chr5_4:20660888-20660907	None:intergenic	20.0%
!!	TAAACATTACTATTTACTGT+CGG	+	chr5_4:20661136-20661155	Msa0843120:intron	20.0%
!!!	AGAATTTTACTAAAGTTTTG+TGG	-	chr5_4:20661311-20661330	None:intergenic	20.0%
!!	CAAAACTTTAGTAAAATTCT+TGG	+	chr5_4:20661311-20661330	Msa0843120:intron	20.0%
!!!	TCCATTTGAAATAAATTGAA+GGG	-	chr5_4:20661398-20661417	None:intergenic	20.0%
!!!	ATCCATTTGAAATAAATTGA+AGG	-	chr5_4:20661399-20661418	None:intergenic	20.0%
!!	TTAAGTTATAAACAGCAAAA+AGG	-	chr5_4:20661633-20661652	None:intergenic	20.0%
!!!	TTTTTGCTGTTTATAACTTA+AGG	+	chr5_4:20661632-20661651	Msa0843120:three_prime_UTR	20.0%
!!!	TTTTGCTGTTTATAACTTAA+GGG	+	chr5_4:20661633-20661652	Msa0843120:three_prime_UTR	20.0%
!!	CATTAAAATCATACATACAA+TGG	-	chr5_4:20661660-20661679	None:intergenic	20.0%
!	CGCATAATATGTATTAATAC+TGG	+	chr5_4:20659707-20659726	Msa0843120:five_prime_UTR	25.0%
!	TGAATAATCGAATAGTGAAA+GGG	-	chr5_4:20660223-20660242	None:intergenic	25.0%
!	ATGAATAATCGAATAGTGAA+AGG	-	chr5_4:20660224-20660243	None:intergenic	25.0%
!!!	CATTTTTGCTGTTGTTAATT+AGG	+	chr5_4:20660264-20660283	Msa0843120:intron	25.0%
!	AATGCTAAATTACTATGTGA+TGG	-	chr5_4:20660545-20660564	None:intergenic	25.0%
!!	ATTTGTTTGCTAAATGATAC+AGG	+	chr5_4:20660573-20660592	Msa0843120:intron	25.0%
!!	TTTGTTTGCTAAATGATACA+GGG	+	chr5_4:20660574-20660593	Msa0843120:intron	25.0%
!	GTATTTAAAGAAAGAAGTTC+AGG	-	chr5_4:20660715-20660734	None:intergenic	25.0%
!	GTATAATTGGTAGATCTAAT+GGG	-	chr5_4:20661369-20661388	None:intergenic	25.0%
!	TGTATAATTGGTAGATCTAA+TGG	-	chr5_4:20661370-20661389	None:intergenic	25.0%
!!	TGAAGGGTAATTTGTATAAT+TGG	-	chr5_4:20661382-20661401	None:intergenic	25.0%
!	ACCCTTCAATTTATTTCAAA+TGG	+	chr5_4:20661394-20661413	Msa0843120:intron	25.0%
!	AAAGGAAACCAATTAAAAAC+AGG	-	chr5_4:20661508-20661527	None:intergenic	25.0%
!!!	TTTTTAATTGGTTTCCTTTG+TGG	+	chr5_4:20661509-20661528	Msa0843120:exon	25.0%
!	TGAAAATTGTCTTCCATAAA+GGG	-	chr5_4:20661556-20661575	None:intergenic	25.0%
!	TTGAAAATTGTCTTCCATAA+AGG	-	chr5_4:20661557-20661576	None:intergenic	25.0%
!!	TATGGAAGACAATTTTCAAA+AGG	+	chr5_4:20661558-20661577	Msa0843120:three_prime_UTR	25.0%
!!	ATGGAAGACAATTTTCAAAA+GGG	+	chr5_4:20661559-20661578	Msa0843120:three_prime_UTR	25.0%
!	CGCATAATATGTATTAATAC+TGG	+	chr5_4:20659707-20659726	Msa0843120:five_prime_UTR	25.0%
!	TGAATAATCGAATAGTGAAA+GGG	-	chr5_4:20660223-20660242	None:intergenic	25.0%
!	ATGAATAATCGAATAGTGAA+AGG	-	chr5_4:20660224-20660243	None:intergenic	25.0%
!!!	CATTTTTGCTGTTGTTAATT+AGG	+	chr5_4:20660264-20660283	Msa0843120:intron	25.0%
!	AATGCTAAATTACTATGTGA+TGG	-	chr5_4:20660545-20660564	None:intergenic	25.0%
!!	ATTTGTTTGCTAAATGATAC+AGG	+	chr5_4:20660573-20660592	Msa0843120:intron	25.0%
!!	TTTGTTTGCTAAATGATACA+GGG	+	chr5_4:20660574-20660593	Msa0843120:intron	25.0%
!	GTATTTAAAGAAAGAAGTTC+AGG	-	chr5_4:20660715-20660734	None:intergenic	25.0%
!	GTATAATTGGTAGATCTAAT+GGG	-	chr5_4:20661369-20661388	None:intergenic	25.0%
!	TGTATAATTGGTAGATCTAA+TGG	-	chr5_4:20661370-20661389	None:intergenic	25.0%
!!	TGAAGGGTAATTTGTATAAT+TGG	-	chr5_4:20661382-20661401	None:intergenic	25.0%
!	ACCCTTCAATTTATTTCAAA+TGG	+	chr5_4:20661394-20661413	Msa0843120:intron	25.0%
!	AAAGGAAACCAATTAAAAAC+AGG	-	chr5_4:20661508-20661527	None:intergenic	25.0%
!!!	TTTTTAATTGGTTTCCTTTG+TGG	+	chr5_4:20661509-20661528	Msa0843120:exon	25.0%
!	TGAAAATTGTCTTCCATAAA+GGG	-	chr5_4:20661556-20661575	None:intergenic	25.0%
!	TTGAAAATTGTCTTCCATAA+AGG	-	chr5_4:20661557-20661576	None:intergenic	25.0%
!!	TATGGAAGACAATTTTCAAA+AGG	+	chr5_4:20661558-20661577	Msa0843120:three_prime_UTR	25.0%
!!	ATGGAAGACAATTTTCAAAA+GGG	+	chr5_4:20661559-20661578	Msa0843120:three_prime_UTR	25.0%
	GGATTCGAATGGATAAAAAA+CGG	+	chr5_4:20660151-20660170	Msa0843120:CDS	30.0%
	GAATAATCGAATAGTGAAAG+GGG	-	chr5_4:20660222-20660241	None:intergenic	30.0%
!	GCTGTTGTTAATTAGGATTT+TGG	+	chr5_4:20660271-20660290	Msa0843120:intron	30.0%
	AAAGTAAGACACCTCTTTAA+AGG	-	chr5_4:20660433-20660452	None:intergenic	30.0%
	TAAATGATACAGGGTCTTAA+AGG	+	chr5_4:20660583-20660602	Msa0843120:intron	30.0%
	AATACTAAATCCCAATGTCA+TGG	+	chr5_4:20660730-20660749	Msa0843120:intron	30.0%
	CAATGTTATAGCTTTCAATG+TGG	-	chr5_4:20660831-20660850	None:intergenic	30.0%
	ATGCATGTAAAGATATTGCA+AGG	-	chr5_4:20660865-20660884	None:intergenic	30.0%
!	AGCACATATCTGTATGTTTT+GGG	+	chr5_4:20660951-20660970	Msa0843120:intron	30.0%
!!!	TGAGTCCAATTTTTTCCTTA+AGG	+	chr5_4:20660983-20661002	Msa0843120:intron	30.0%
	TATCTATAATTGCTTGGTCA+AGG	+	chr5_4:20661172-20661191	Msa0843120:intron	30.0%
	ACAAGAATTCTAGCATGTTA+TGG	-	chr5_4:20661342-20661361	None:intergenic	30.0%
	TGCAAAATTTGATCCCTTTA+TGG	+	chr5_4:20661540-20661559	Msa0843120:three_prime_UTR	30.0%
	GGATTCGAATGGATAAAAAA+CGG	+	chr5_4:20660151-20660170	Msa0843120:CDS	30.0%
	GAATAATCGAATAGTGAAAG+GGG	-	chr5_4:20660222-20660241	None:intergenic	30.0%
!	GCTGTTGTTAATTAGGATTT+TGG	+	chr5_4:20660271-20660290	Msa0843120:intron	30.0%
	AAAGTAAGACACCTCTTTAA+AGG	-	chr5_4:20660433-20660452	None:intergenic	30.0%
	TAAATGATACAGGGTCTTAA+AGG	+	chr5_4:20660583-20660602	Msa0843120:intron	30.0%
	AATACTAAATCCCAATGTCA+TGG	+	chr5_4:20660730-20660749	Msa0843120:intron	30.0%
	CAATGTTATAGCTTTCAATG+TGG	-	chr5_4:20660831-20660850	None:intergenic	30.0%
	ATGCATGTAAAGATATTGCA+AGG	-	chr5_4:20660865-20660884	None:intergenic	30.0%
!	AGCACATATCTGTATGTTTT+GGG	+	chr5_4:20660951-20660970	Msa0843120:intron	30.0%
!!!	TGAGTCCAATTTTTTCCTTA+AGG	+	chr5_4:20660983-20661002	Msa0843120:intron	30.0%
	TATCTATAATTGCTTGGTCA+AGG	+	chr5_4:20661172-20661191	Msa0843120:intron	30.0%
	ACAAGAATTCTAGCATGTTA+TGG	-	chr5_4:20661342-20661361	None:intergenic	30.0%
	TGCAAAATTTGATCCCTTTA+TGG	+	chr5_4:20661540-20661559	Msa0843120:three_prime_UTR	30.0%
	TATGTCTATTTCGTCGATGT+CGG	-	chr5_4:20659992-20660011	None:intergenic	35.0%
	AATAGACATATCCAATCACC+CGG	+	chr5_4:20660002-20660021	Msa0843120:CDS	35.0%
	TCCTTTCTGTTCTCTTTAGT+CGG	+	chr5_4:20660124-20660143	Msa0843120:intron	35.0%
!	CGATCTGATTTTCATGATTC+AGG	+	chr5_4:20660183-20660202	Msa0843120:CDS	35.0%
	ATTACATACCTGAGTAGCAA+TGG	-	chr5_4:20660314-20660333	None:intergenic	35.0%
!	AGCAACCAAAACCTTTAAAG+AGG	+	chr5_4:20660419-20660438	Msa0843120:CDS	35.0%
!!	AGACACCTCTTTAAAGGTTT+TGG	-	chr5_4:20660427-20660446	None:intergenic	35.0%
	ATGTGGAGTTCTTGAGTAAT+TGG	-	chr5_4:20660458-20660477	None:intergenic	35.0%
	TAGAAAAAGACCTTGAGATG+TGG	-	chr5_4:20660475-20660494	None:intergenic	35.0%
	CAAATGACGAAAAATAGGAC+AGG	-	chr5_4:20660689-20660708	None:intergenic	35.0%
!!	GTCCTATTTTTCGTCATTTG+TGG	+	chr5_4:20660689-20660708	Msa0843120:intron	35.0%
	TTTATAGCGCTTAAGCAAGA+AGG	+	chr5_4:20660920-20660939	Msa0843120:intron	35.0%
!	GAGCACATATCTGTATGTTT+TGG	+	chr5_4:20660950-20660969	Msa0843120:intron	35.0%
	CTCTGCCTTAAGGAAAAAAT+TGG	-	chr5_4:20660991-20661010	None:intergenic	35.0%
	CTTCTCTTGCAATATTCATG+TGG	-	chr5_4:20661044-20661063	None:intergenic	35.0%
	CATGAATATTGCAAGAGAAG+AGG	+	chr5_4:20661044-20661063	Msa0843120:intron	35.0%
!	ATGTTATGCTACAAGTGTTG+CGG	-	chr5_4:20661238-20661257	None:intergenic	35.0%
	GTAGCATAACATGTTAGAGT+TGG	+	chr5_4:20661246-20661265	Msa0843120:intron	35.0%
!	ATACGGATATTATTGGTCTC+AGG	-	chr5_4:20661283-20661302	None:intergenic	35.0%
!!	AAAGTTTTGTGGCTCAGATA+CGG	-	chr5_4:20661300-20661319	None:intergenic	35.0%
!!!	GTGCACATCCTGTTTTTAAT+TGG	+	chr5_4:20661497-20661516	Msa0843120:exon	35.0%
	TATGTCTATTTCGTCGATGT+CGG	-	chr5_4:20659992-20660011	None:intergenic	35.0%
	AATAGACATATCCAATCACC+CGG	+	chr5_4:20660002-20660021	Msa0843120:CDS	35.0%
	TCCTTTCTGTTCTCTTTAGT+CGG	+	chr5_4:20660124-20660143	Msa0843120:intron	35.0%
!	CGATCTGATTTTCATGATTC+AGG	+	chr5_4:20660183-20660202	Msa0843120:CDS	35.0%
	ATTACATACCTGAGTAGCAA+TGG	-	chr5_4:20660314-20660333	None:intergenic	35.0%
!	AGCAACCAAAACCTTTAAAG+AGG	+	chr5_4:20660419-20660438	Msa0843120:CDS	35.0%
!!	AGACACCTCTTTAAAGGTTT+TGG	-	chr5_4:20660427-20660446	None:intergenic	35.0%
	ATGTGGAGTTCTTGAGTAAT+TGG	-	chr5_4:20660458-20660477	None:intergenic	35.0%
	TAGAAAAAGACCTTGAGATG+TGG	-	chr5_4:20660475-20660494	None:intergenic	35.0%
	CAAATGACGAAAAATAGGAC+AGG	-	chr5_4:20660689-20660708	None:intergenic	35.0%
!!	GTCCTATTTTTCGTCATTTG+TGG	+	chr5_4:20660689-20660708	Msa0843120:intron	35.0%
	TTTATAGCGCTTAAGCAAGA+AGG	+	chr5_4:20660920-20660939	Msa0843120:intron	35.0%
!	GAGCACATATCTGTATGTTT+TGG	+	chr5_4:20660950-20660969	Msa0843120:intron	35.0%
	CTCTGCCTTAAGGAAAAAAT+TGG	-	chr5_4:20660991-20661010	None:intergenic	35.0%
	CTTCTCTTGCAATATTCATG+TGG	-	chr5_4:20661044-20661063	None:intergenic	35.0%
	CATGAATATTGCAAGAGAAG+AGG	+	chr5_4:20661044-20661063	Msa0843120:intron	35.0%
!	ATGTTATGCTACAAGTGTTG+CGG	-	chr5_4:20661238-20661257	None:intergenic	35.0%
	GTAGCATAACATGTTAGAGT+TGG	+	chr5_4:20661246-20661265	Msa0843120:intron	35.0%
!	ATACGGATATTATTGGTCTC+AGG	-	chr5_4:20661283-20661302	None:intergenic	35.0%
!!	AAAGTTTTGTGGCTCAGATA+CGG	-	chr5_4:20661300-20661319	None:intergenic	35.0%
!!!	GTGCACATCCTGTTTTTAAT+TGG	+	chr5_4:20661497-20661516	Msa0843120:exon	35.0%
!	GAATCTTTTTGCGACGCTAT+CGG	-	chr5_4:20659890-20659909	None:intergenic	40.0%
	ATAGCGTCGCAAAAAGATTC+AGG	+	chr5_4:20659890-20659909	Msa0843120:CDS	40.0%
	CTAAAGAGAACAGAAAGGAG+TGG	-	chr5_4:20660123-20660142	None:intergenic	40.0%
	ACCGACTAAAGAGAACAGAA+AGG	-	chr5_4:20660128-20660147	None:intergenic	40.0%
	CTGTTCTCTTTAGTCGGTAT+TGG	+	chr5_4:20660130-20660149	Msa0843120:intron	40.0%
!	TAGTCGGTATTGGATTCGAA+TGG	+	chr5_4:20660140-20660159	Msa0843120:intron	40.0%
	TCGAAATACCATTGCTACTC+AGG	+	chr5_4:20660303-20660322	Msa0843120:CDS	40.0%
	ACCTATCTTCAGCAAACCAA+CGG	+	chr5_4:20660616-20660635	Msa0843120:CDS	40.0%
	GGCCACAAATGACGAAAAAT+AGG	-	chr5_4:20660694-20660713	None:intergenic	40.0%
	ATCCCAATGTCATGGTATGA+TGG	+	chr5_4:20660738-20660757	Msa0843120:intron	40.0%
	CACCATCATACCATGACATT+GGG	-	chr5_4:20660743-20660762	None:intergenic	40.0%
	GTTATAGCTTTCAATGTGGC+AGG	-	chr5_4:20660827-20660846	None:intergenic	40.0%
	AACATACAGATATGTGCTCG+TGG	-	chr5_4:20660949-20660968	None:intergenic	40.0%
	GAATATTGCAAGAGAAGAGG+AGG	+	chr5_4:20661047-20661066	Msa0843120:intron	40.0%
	GGCTCAGATACGGATATTAT+TGG	-	chr5_4:20661290-20661309	None:intergenic	40.0%
	GTTTATTCGACACCAGTAGT+AGG	+	chr5_4:20661586-20661605	Msa0843120:three_prime_UTR	40.0%
	ATATCGCCATTTCCTACTAC+TGG	-	chr5_4:20661601-20661620	None:intergenic	40.0%
!	GAATCTTTTTGCGACGCTAT+CGG	-	chr5_4:20659890-20659909	None:intergenic	40.0%
	ATAGCGTCGCAAAAAGATTC+AGG	+	chr5_4:20659890-20659909	Msa0843120:CDS	40.0%
	CTAAAGAGAACAGAAAGGAG+TGG	-	chr5_4:20660123-20660142	None:intergenic	40.0%
	ACCGACTAAAGAGAACAGAA+AGG	-	chr5_4:20660128-20660147	None:intergenic	40.0%
	CTGTTCTCTTTAGTCGGTAT+TGG	+	chr5_4:20660130-20660149	Msa0843120:intron	40.0%
!	TAGTCGGTATTGGATTCGAA+TGG	+	chr5_4:20660140-20660159	Msa0843120:intron	40.0%
	TCGAAATACCATTGCTACTC+AGG	+	chr5_4:20660303-20660322	Msa0843120:CDS	40.0%
	ACCTATCTTCAGCAAACCAA+CGG	+	chr5_4:20660616-20660635	Msa0843120:CDS	40.0%
	GGCCACAAATGACGAAAAAT+AGG	-	chr5_4:20660694-20660713	None:intergenic	40.0%
	ATCCCAATGTCATGGTATGA+TGG	+	chr5_4:20660738-20660757	Msa0843120:intron	40.0%
	CACCATCATACCATGACATT+GGG	-	chr5_4:20660743-20660762	None:intergenic	40.0%
	GTTATAGCTTTCAATGTGGC+AGG	-	chr5_4:20660827-20660846	None:intergenic	40.0%
	AACATACAGATATGTGCTCG+TGG	-	chr5_4:20660949-20660968	None:intergenic	40.0%
	GAATATTGCAAGAGAAGAGG+AGG	+	chr5_4:20661047-20661066	Msa0843120:intron	40.0%
	GGCTCAGATACGGATATTAT+TGG	-	chr5_4:20661290-20661309	None:intergenic	40.0%
	GTTTATTCGACACCAGTAGT+AGG	+	chr5_4:20661586-20661605	Msa0843120:three_prime_UTR	40.0%
	ATATCGCCATTTCCTACTAC+TGG	-	chr5_4:20661601-20661620	None:intergenic	40.0%
	CTCAAGAACTCCACATCTCA+AGG	+	chr5_4:20660462-20660481	Msa0843120:CDS	45.0%
	CCTATCTTCAGCAAACCAAC+GGG	+	chr5_4:20660617-20660636	Msa0843120:CDS	45.0%
	AACCAACGGGTACTTTAGAC+AGG	+	chr5_4:20660630-20660649	Msa0843120:CDS	45.0%
	ACTTTAGACAGGTCAGCTTC+TGG	+	chr5_4:20660641-20660660	Msa0843120:intron	45.0%
	CCACCATCATACCATGACAT+TGG	-	chr5_4:20660744-20660763	None:intergenic	45.0%
	CCAATGTCATGGTATGATGG+TGG	+	chr5_4:20660741-20660760	Msa0843120:intron	45.0%
	ATAGCTTTCAATGTGGCAGG+AGG	-	chr5_4:20660824-20660843	None:intergenic	45.0%
	CTTAAGGCAGAGAGACTAGA+GGG	+	chr5_4:20660999-20661018	Msa0843120:intron	45.0%
	TTGCAAGAGAAGAGGAGGAT+TGG	+	chr5_4:20661052-20661071	Msa0843120:intron	45.0%
	ATTGCTTGGTCAAGGAGAGA+AGG	+	chr5_4:20661180-20661199	Msa0843120:intron	45.0%
	GAAGCTGTCGATACTGAGTA+TGG	-	chr5_4:20661210-20661229	None:intergenic	45.0%
!	TTATGCTACAAGTGTTGCGG+TGG	-	chr5_4:20661235-20661254	None:intergenic	45.0%
	ATACCAGTAGATCCACTGCT+TGG	-	chr5_4:20661477-20661496	None:intergenic	45.0%
	TGGTTTCCTTTGTGGAGTTG+AGG	+	chr5_4:20661517-20661536	Msa0843120:three_prime_UTR	45.0%
	TTTGCACCTCAACTCCACAA+AGG	-	chr5_4:20661526-20661545	None:intergenic	45.0%
	TCGACACCAGTAGTAGGAAA+TGG	+	chr5_4:20661592-20661611	Msa0843120:three_prime_UTR	45.0%
	CTCAAGAACTCCACATCTCA+AGG	+	chr5_4:20660462-20660481	Msa0843120:CDS	45.0%
	CCTATCTTCAGCAAACCAAC+GGG	+	chr5_4:20660617-20660636	Msa0843120:CDS	45.0%
	AACCAACGGGTACTTTAGAC+AGG	+	chr5_4:20660630-20660649	Msa0843120:CDS	45.0%
	ACTTTAGACAGGTCAGCTTC+TGG	+	chr5_4:20660641-20660660	Msa0843120:intron	45.0%
	CCACCATCATACCATGACAT+TGG	-	chr5_4:20660744-20660763	None:intergenic	45.0%
	CCAATGTCATGGTATGATGG+TGG	+	chr5_4:20660741-20660760	Msa0843120:intron	45.0%
	ATAGCTTTCAATGTGGCAGG+AGG	-	chr5_4:20660824-20660843	None:intergenic	45.0%
	CTTAAGGCAGAGAGACTAGA+GGG	+	chr5_4:20660999-20661018	Msa0843120:intron	45.0%
	TTGCAAGAGAAGAGGAGGAT+TGG	+	chr5_4:20661052-20661071	Msa0843120:intron	45.0%
	ATTGCTTGGTCAAGGAGAGA+AGG	+	chr5_4:20661180-20661199	Msa0843120:intron	45.0%
	GAAGCTGTCGATACTGAGTA+TGG	-	chr5_4:20661210-20661229	None:intergenic	45.0%
!	TTATGCTACAAGTGTTGCGG+TGG	-	chr5_4:20661235-20661254	None:intergenic	45.0%
	ATACCAGTAGATCCACTGCT+TGG	-	chr5_4:20661477-20661496	None:intergenic	45.0%
	TGGTTTCCTTTGTGGAGTTG+AGG	+	chr5_4:20661517-20661536	Msa0843120:three_prime_UTR	45.0%
	TTTGCACCTCAACTCCACAA+AGG	-	chr5_4:20661526-20661545	None:intergenic	45.0%
	TCGACACCAGTAGTAGGAAA+TGG	+	chr5_4:20661592-20661611	Msa0843120:three_prime_UTR	45.0%
	GGCAAAGAAACGAAGCAGGA+TGG	+	chr5_4:20659786-20659805	Msa0843120:exon	50.0%
	GAGATATCTCGCGCTATCGT+TGG	+	chr5_4:20659859-20659878	Msa0843120:CDS	50.0%
!!	AGAAGGAGGTATCTCCTCGT+CGG	-	chr5_4:20659926-20659945	None:intergenic	50.0%
	TCTATTTCGTCGATGTCGGC+GGG	-	chr5_4:20659988-20660007	None:intergenic	50.0%
	GTCTATTTCGTCGATGTCGG+CGG	-	chr5_4:20659989-20660008	None:intergenic	50.0%
	AGACATATCCAATCACCCGG+TGG	+	chr5_4:20660005-20660024	Msa0843120:CDS	50.0%
	ATGACGATGCAACGAACCAC+CGG	-	chr5_4:20660024-20660043	None:intergenic	50.0%
!!	CCCGTTGGTTTGCTGAAGAT+AGG	-	chr5_4:20660620-20660639	None:intergenic	50.0%
!	GACCTGTCTAAAGTACCCGT+TGG	-	chr5_4:20660635-20660654	None:intergenic	50.0%
	GGCAGGAGGAGTGAAGAATT+TGG	-	chr5_4:20660810-20660829	None:intergenic	50.0%
	CCTCTAGTCTCTCTGCCTTA+AGG	-	chr5_4:20661001-20661020	None:intergenic	50.0%
	CCTTAAGGCAGAGAGACTAG+AGG	+	chr5_4:20660998-20661017	Msa0843120:intron	50.0%
	CAAATGGATCTGCCAACTGC+AGG	+	chr5_4:20661410-20661429	Msa0843120:intron	50.0%
	AGAGCTGTTGTTCCAAGCAG+TGG	+	chr5_4:20661462-20661481	Msa0843120:CDS	50.0%
	GTTCCAAGCAGTGGATCTAC+TGG	+	chr5_4:20661471-20661490	Msa0843120:CDS	50.0%
	GGCAAAGAAACGAAGCAGGA+TGG	+	chr5_4:20659786-20659805	Msa0843120:exon	50.0%
	GAGATATCTCGCGCTATCGT+TGG	+	chr5_4:20659859-20659878	Msa0843120:CDS	50.0%
!!	AGAAGGAGGTATCTCCTCGT+CGG	-	chr5_4:20659926-20659945	None:intergenic	50.0%
	TCTATTTCGTCGATGTCGGC+GGG	-	chr5_4:20659988-20660007	None:intergenic	50.0%
	GTCTATTTCGTCGATGTCGG+CGG	-	chr5_4:20659989-20660008	None:intergenic	50.0%
	AGACATATCCAATCACCCGG+TGG	+	chr5_4:20660005-20660024	Msa0843120:CDS	50.0%
	ATGACGATGCAACGAACCAC+CGG	-	chr5_4:20660024-20660043	None:intergenic	50.0%
!!	CCCGTTGGTTTGCTGAAGAT+AGG	-	chr5_4:20660620-20660639	None:intergenic	50.0%
!	GACCTGTCTAAAGTACCCGT+TGG	-	chr5_4:20660635-20660654	None:intergenic	50.0%
	GGCAGGAGGAGTGAAGAATT+TGG	-	chr5_4:20660810-20660829	None:intergenic	50.0%
	CCTCTAGTCTCTCTGCCTTA+AGG	-	chr5_4:20661001-20661020	None:intergenic	50.0%
	CCTTAAGGCAGAGAGACTAG+AGG	+	chr5_4:20660998-20661017	Msa0843120:intron	50.0%
	CAAATGGATCTGCCAACTGC+AGG	+	chr5_4:20661410-20661429	Msa0843120:intron	50.0%
	AGAGCTGTTGTTCCAAGCAG+TGG	+	chr5_4:20661462-20661481	Msa0843120:CDS	50.0%
	GTTCCAAGCAGTGGATCTAC+TGG	+	chr5_4:20661471-20661490	Msa0843120:CDS	50.0%
	ACGCGGCAAAGAAACGAAGC+AGG	+	chr5_4:20659782-20659801	Msa0843120:five_prime_UTR	55.0%
	TATCCCGTTCTCGCCGTTGT+CGG	-	chr5_4:20659812-20659831	None:intergenic	55.0%
	AGCGCGAGATATCTCGTCGT+CGG	-	chr5_4:20659854-20659873	None:intergenic	55.0%
!!	AAGGAGGTATCTCCTCGTCG+GGG	-	chr5_4:20659924-20659943	None:intergenic	55.0%
!!	GAAGGAGGTATCTCCTCGTC+GGG	-	chr5_4:20659925-20659944	None:intergenic	55.0%
	TGACGATGCAACGAACCACC+GGG	-	chr5_4:20660023-20660042	None:intergenic	55.0%
	GAGAGACTAGAGGGAGAGAG+AGG	+	chr5_4:20661008-20661027	Msa0843120:intron	55.0%
!	TGCTACAAGTGTTGCGGTGG+AGG	-	chr5_4:20661232-20661251	None:intergenic	55.0%
	GCTGCTCTACTTCCTGCAGT+TGG	-	chr5_4:20661425-20661444	None:intergenic	55.0%
	GCAGGAAGTAGAGCAGCATC+AGG	+	chr5_4:20661428-20661447	Msa0843120:intron	55.0%
	ACGCGGCAAAGAAACGAAGC+AGG	+	chr5_4:20659782-20659801	Msa0843120:five_prime_UTR	55.0%
	TATCCCGTTCTCGCCGTTGT+CGG	-	chr5_4:20659812-20659831	None:intergenic	55.0%
	AGCGCGAGATATCTCGTCGT+CGG	-	chr5_4:20659854-20659873	None:intergenic	55.0%
!!	AAGGAGGTATCTCCTCGTCG+GGG	-	chr5_4:20659924-20659943	None:intergenic	55.0%
!!	GAAGGAGGTATCTCCTCGTC+GGG	-	chr5_4:20659925-20659944	None:intergenic	55.0%
	TGACGATGCAACGAACCACC+GGG	-	chr5_4:20660023-20660042	None:intergenic	55.0%
	GAGAGACTAGAGGGAGAGAG+AGG	+	chr5_4:20661008-20661027	Msa0843120:intron	55.0%
!	TGCTACAAGTGTTGCGGTGG+AGG	-	chr5_4:20661232-20661251	None:intergenic	55.0%
	GCTGCTCTACTTCCTGCAGT+TGG	-	chr5_4:20661425-20661444	None:intergenic	55.0%
	GCAGGAAGTAGAGCAGCATC+AGG	+	chr5_4:20661428-20661447	Msa0843120:intron	55.0%
	CGAAGCAGGATGGCCGACAA+CGG	+	chr5_4:20659796-20659815	Msa0843120:exon	60.0%
	ATGGCCGACAACGGCGAGAA+CGG	+	chr5_4:20659805-20659824	Msa0843120:CDS	60.0%
	GTCGGCGCGAGCGATTAAAC+GGG	-	chr5_4:20659836-20659855	None:intergenic	60.0%
	CGTCGGCGCGAGCGATTAAA+CGG	-	chr5_4:20659837-20659856	None:intergenic	60.0%
	GGTATCTCCTCGTCGGGGTT+GGG	-	chr5_4:20659919-20659938	None:intergenic	60.0%
	AGGTATCTCCTCGTCGGGGT+TGG	-	chr5_4:20659920-20659939	None:intergenic	60.0%
	GGAGAGAGGGAGAGAGGAGA+CGG	-	chr5_4:20659959-20659978	None:intergenic	60.0%
	AGTGAGGGAGAGAGGGAGAG+AGG	-	chr5_4:20659965-20659984	None:intergenic	60.0%
	GCAACGAACCACCGGGTGAT+TGG	-	chr5_4:20660016-20660035	None:intergenic	60.0%
	CGAAGCAGGATGGCCGACAA+CGG	+	chr5_4:20659796-20659815	Msa0843120:exon	60.0%
	ATGGCCGACAACGGCGAGAA+CGG	+	chr5_4:20659805-20659824	Msa0843120:CDS	60.0%
	GTCGGCGCGAGCGATTAAAC+GGG	-	chr5_4:20659836-20659855	None:intergenic	60.0%
	CGTCGGCGCGAGCGATTAAA+CGG	-	chr5_4:20659837-20659856	None:intergenic	60.0%
	GGTATCTCCTCGTCGGGGTT+GGG	-	chr5_4:20659919-20659938	None:intergenic	60.0%
	AGGTATCTCCTCGTCGGGGT+TGG	-	chr5_4:20659920-20659939	None:intergenic	60.0%
	GGAGAGAGGGAGAGAGGAGA+CGG	-	chr5_4:20659959-20659978	None:intergenic	60.0%
	AGTGAGGGAGAGAGGGAGAG+AGG	-	chr5_4:20659965-20659984	None:intergenic	60.0%
	GCAACGAACCACCGGGTGAT+TGG	-	chr5_4:20660016-20660035	None:intergenic	60.0%
	TCAGCGTTGCGTGGGTGGGT+CGG	-	chr5_4:20659763-20659782	None:intergenic	65.0%
	TTGCCGCGTCAGCGTTGCGT+GGG	-	chr5_4:20659771-20659790	None:intergenic	65.0%
	TTTGCCGCGTCAGCGTTGCG+TGG	-	chr5_4:20659772-20659791	None:intergenic	65.0%
	TGGCCGACAACGGCGAGAAC+GGG	+	chr5_4:20659806-20659825	Msa0843120:CDS	65.0%
	AGAGAGGAGACGGCGGGAAC+GGG	-	chr5_4:20659949-20659968	None:intergenic	65.0%
	GAGAGAGGAGACGGCGGGAA+CGG	-	chr5_4:20659950-20659969	None:intergenic	65.0%
	AGAGGGAGAGAGGAGACGGC+GGG	-	chr5_4:20659955-20659974	None:intergenic	65.0%
	GAGAGGGAGAGAGGAGACGG+CGG	-	chr5_4:20659956-20659975	None:intergenic	65.0%
	GTCGATGTCGGCGGGAGTGA+GGG	-	chr5_4:20659980-20659999	None:intergenic	65.0%
	TCAGCGTTGCGTGGGTGGGT+CGG	-	chr5_4:20659763-20659782	None:intergenic	65.0%
	TTGCCGCGTCAGCGTTGCGT+GGG	-	chr5_4:20659771-20659790	None:intergenic	65.0%
	TTTGCCGCGTCAGCGTTGCG+TGG	-	chr5_4:20659772-20659791	None:intergenic	65.0%
	TGGCCGACAACGGCGAGAAC+GGG	+	chr5_4:20659806-20659825	Msa0843120:CDS	65.0%
	AGAGAGGAGACGGCGGGAAC+GGG	-	chr5_4:20659949-20659968	None:intergenic	65.0%
	GAGAGAGGAGACGGCGGGAA+CGG	-	chr5_4:20659950-20659969	None:intergenic	65.0%
	AGAGGGAGAGAGGAGACGGC+GGG	-	chr5_4:20659955-20659974	None:intergenic	65.0%
	GAGAGGGAGAGAGGAGACGG+CGG	-	chr5_4:20659956-20659975	None:intergenic	65.0%
	GTCGATGTCGGCGGGAGTGA+GGG	-	chr5_4:20659980-20659999	None:intergenic	65.0%
	CAGCGTTGCGTGGGTGGGTC+GGG	-	chr5_4:20659762-20659781	None:intergenic	70.0%
	CGCGTCAGCGTTGCGTGGGT+GGG	-	chr5_4:20659767-20659786	None:intergenic	70.0%
	CCACCCACGCAACGCTGACG+CGG	+	chr5_4:20659765-20659784	Msa0843120:five_prime_UTR	70.0%
	CAGGAAGCCCAACCCCGACG+AGG	+	chr5_4:20659909-20659928	Msa0843120:CDS	70.0%
	ACGGCGGGAACGGGAGAAGG+AGG	-	chr5_4:20659940-20659959	None:intergenic	70.0%
	GAGACGGCGGGAACGGGAGA+AGG	-	chr5_4:20659943-20659962	None:intergenic	70.0%
	CGGCGGGAGTGAGGGAGAGA+GGG	-	chr5_4:20659972-20659991	None:intergenic	70.0%
	TCGGCGGGAGTGAGGGAGAG+AGG	-	chr5_4:20659973-20659992	None:intergenic	70.0%
	CGTCGATGTCGGCGGGAGTG+AGG	-	chr5_4:20659981-20660000	None:intergenic	70.0%
	CAGCGTTGCGTGGGTGGGTC+GGG	-	chr5_4:20659762-20659781	None:intergenic	70.0%
	CGCGTCAGCGTTGCGTGGGT+GGG	-	chr5_4:20659767-20659786	None:intergenic	70.0%
	CCACCCACGCAACGCTGACG+CGG	+	chr5_4:20659765-20659784	Msa0843120:five_prime_UTR	70.0%
	CAGGAAGCCCAACCCCGACG+AGG	+	chr5_4:20659909-20659928	Msa0843120:CDS	70.0%
	ACGGCGGGAACGGGAGAAGG+AGG	-	chr5_4:20659940-20659959	None:intergenic	70.0%
	GAGACGGCGGGAACGGGAGA+AGG	-	chr5_4:20659943-20659962	None:intergenic	70.0%
	CGGCGGGAGTGAGGGAGAGA+GGG	-	chr5_4:20659972-20659991	None:intergenic	70.0%
	TCGGCGGGAGTGAGGGAGAG+AGG	-	chr5_4:20659973-20659992	None:intergenic	70.0%
	CGTCGATGTCGGCGGGAGTG+AGG	-	chr5_4:20659981-20660000	None:intergenic	70.0%
	CCGCGTCAGCGTTGCGTGGG+TGG	-	chr5_4:20659768-20659787	None:intergenic	75.0%
	CCGCGTCAGCGTTGCGTGGG+TGG	-	chr5_4:20659768-20659787	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	20659679	20661710	20659679	ID=Msa0843120;Name=Msa0843120
chr5_4	mRNA	20659679	20661710	20659679	ID=Msa0843120-mRNA-1;Parent=Msa0843120;Name=Msa0843120-mRNA-1;_AED=0.22;_eAED=0.22;_QI=126\|1\|1\|1\|1\|1\|6\|195\|186
chr5_4	exon	20659679	20660054	20659679	ID=Msa0843120-mRNA-1:exon:8513;Parent=Msa0843120-mRNA-1
chr5_4	exon	20660143	20660204	20660143	ID=Msa0843120-mRNA-1:exon:8514;Parent=Msa0843120-mRNA-1
chr5_4	exon	20660286	20660324	20660286	ID=Msa0843120-mRNA-1:exon:8515;Parent=Msa0843120-mRNA-1
chr5_4	exon	20660415	20660483	20660415	ID=Msa0843120-mRNA-1:exon:8516;Parent=Msa0843120-mRNA-1
chr5_4	exon	20660595	20660651	20660595	ID=Msa0843120-mRNA-1:exon:8517;Parent=Msa0843120-mRNA-1
chr5_4	exon	20661432	20661710	20661432	ID=Msa0843120-mRNA-1:exon:8518;Parent=Msa0843120-mRNA-1
chr5_4	five_prime_UTR	20659679	20659804	20659679	ID=Msa0843120-mRNA-1:five_prime_utr;Parent=Msa0843120-mRNA-1
chr5_4	CDS	20659805	20660054	20659805	ID=Msa0843120-mRNA-1:cds;Parent=Msa0843120-mRNA-1
chr5_4	CDS	20660143	20660204	20660143	ID=Msa0843120-mRNA-1:cds;Parent=Msa0843120-mRNA-1
chr5_4	CDS	20660286	20660324	20660286	ID=Msa0843120-mRNA-1:cds;Parent=Msa0843120-mRNA-1
chr5_4	CDS	20660415	20660483	20660415	ID=Msa0843120-mRNA-1:cds;Parent=Msa0843120-mRNA-1
chr5_4	CDS	20660595	20660651	20660595	ID=Msa0843120-mRNA-1:cds;Parent=Msa0843120-mRNA-1
chr5_4	CDS	20661432	20661515	20661432	ID=Msa0843120-mRNA-1:cds;Parent=Msa0843120-mRNA-1
chr5_4	three_prime_UTR	20661516	20661710	20661516	ID=Msa0843120-mRNA-1:three_prime_utr;Parent=Msa0843120-mRNA-1

Gene Sequence

>Msa0843120

ATGGCCGACAACGGCGAGAACGGGATATCCCGTTTAATCGCTCGCGCCGACGACGAGATATCTCGCGCTATCGTTGGTATCGCCGATAGCGTCGCAAAAAGATTCAGGAAGCCCAACCCCGACGAGGAGATACCTCCTTCTCCCGTTCCCGCCGTCTCCTCTCTCCCTCTCTCCCTCACTCCCGCCGACATCGACGAAATAGACATATCCAATCACCCGGTGGTTCGTTGCATCGTCATATTTACAGCTTTCGGTATTGGATTCGAATGGATAAAAAACGGCTACTTTCTCGATCTGATTTTCATGATTCAGGATTTTGGCAAAGCATTTCGAAATACCATTGCTACTCAGGTTCAGCAACCAAAACCTTTAAAGAGGTGTCTTACTTTCTCCAATTACTCAAGAACTCCACATCTCAAGGGTCTTAAAGGCATGAGAGAAACCTATCTTCAGCAAACCAACGGGTACTTTAGACAGGAAGTAGAGCAGCATCAGGAAGACACATCTAGAGCTGTTGTTCCAAGCAGTGGATCTACTGGTATGTGCACATCCTGTTTTTAA

Protein sequence

>Msa0843120

MADNGENGISRLIARADDEISRAIVGIADSVAKRFRKPNPDEEIPPSPVPAVSSLPLSLTPADIDEIDISNHPVVRCIVIFTAFGIGFEWIKNGYFLDLIFMIQDFGKAFRNTIATQVQQPKPLKRCLTFSNYSRTPHLKGLKGMRETYLQQTNGYFRQEVEQHQEDTSRAVVPSSGSTGMCTSCF*