Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0661040 | GAU40685.1 | 53.205 | 156 | 23 | 2 | 6 | 112 | 2 | 156 | 2.76e-42 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005500 | Msa0661040 | 0.804208 | 2.412156e-49 | -8.615850e-47 |
| Msa0011060 | Msa0661040 | 0.813079 | 3.068441e-51 | -8.615850e-47 |
| Msa0013040 | Msa0661040 | -0.811133 | 8.153024e-51 | -8.615850e-47 |
| Msa0043120 | Msa0661040 | -0.804302 | 2.305336e-49 | -8.615850e-47 |
| Msa0048410 | Msa0661040 | 0.824696 | 7.019333e-54 | -8.615850e-47 |
| Msa0049670 | Msa0661040 | 0.815729 | 7.964814e-52 | -8.615850e-47 |
| Msa0050320 | Msa0661040 | 0.817946 | 2.532517e-52 | -8.615850e-47 |
| Msa0060080 | Msa0661040 | 0.837354 | 5.463228e-57 | -8.615850e-47 |
| Msa0067750 | Msa0661040 | 0.804484 | 2.112524e-49 | -8.615850e-47 |
| Msa0081700 | Msa0661040 | 0.825855 | 3.732984e-54 | -8.615850e-47 |
| Msa0099090 | Msa0661040 | 0.804463 | 2.134366e-49 | -8.615850e-47 |
| Msa0104900 | Msa0661040 | 0.818924 | 1.520335e-52 | -8.615850e-47 |
| Msa0110350 | Msa0661040 | 0.806875 | 6.652624e-50 | -8.615850e-47 |
| Msa0610240 | Msa0661040 | 0.801177 | 1.018452e-48 | -8.615850e-47 |
| Msa0614060 | Msa0661040 | 0.802138 | 6.468453e-49 | -8.615850e-47 |
| Msa0617470 | Msa0661040 | 0.806662 | 7.378541e-50 | -8.615850e-47 |
| Msa0625550 | Msa0661040 | 0.815777 | 7.769005e-52 | -8.615850e-47 |
| Msa0632040 | Msa0661040 | 0.806280 | 8.881183e-50 | -8.615850e-47 |
| Msa0633630 | Msa0661040 | -0.809617 | 1.731782e-50 | -8.615850e-47 |
| Msa0635070 | Msa0661040 | 0.820831 | 5.571363e-53 | -8.615850e-47 |
| Msa0638550 | Msa0661040 | 0.801231 | 9.927414e-49 | -8.615850e-47 |
| Msa0651340 | Msa0661040 | 0.822611 | 2.159748e-53 | -8.615850e-47 |
| Msa0654400 | Msa0661040 | 0.811630 | 6.359639e-51 | -8.615850e-47 |
| Msa0654500 | Msa0661040 | 0.813081 | 3.066125e-51 | -8.615850e-47 |
| Msa0661040 | Msa0670360 | -0.803732 | 3.029098e-49 | -8.615850e-47 |
| Msa0661040 | Msa0685810 | 0.803662 | 3.133041e-49 | -8.615850e-47 |
| Msa0661040 | Msa0700250 | 0.805696 | 1.178687e-49 | -8.615850e-47 |
| Msa0661040 | Msa0700870 | 0.804323 | 2.282230e-49 | -8.615850e-47 |
| Msa0661040 | Msa0700930 | 0.801914 | 7.191035e-49 | -8.615850e-47 |
| Msa0661040 | Msa0703430 | 0.800319 | 1.523589e-48 | -8.615850e-47 |
| Msa0661040 | Msa0705710 | 0.810439 | 1.151862e-50 | -8.615850e-47 |
| Msa0661040 | Msa0741600 | 0.827982 | 1.157868e-54 | -8.615850e-47 |
| Msa0661040 | Msa0742690 | 0.801195 | 1.009727e-48 | -8.615850e-47 |
| Msa0661040 | Msa0754190 | 0.812558 | 3.990568e-51 | -8.615850e-47 |
| Msa0661040 | Msa0779830 | 0.825330 | 4.971169e-54 | -8.615850e-47 |
| Msa0661040 | Msa0791700 | 0.837294 | 5.658460e-57 | -8.615850e-47 |
| Msa0661040 | Msa0807130 | 0.810232 | 1.277134e-50 | -8.615850e-47 |
| Msa0661040 | Msa0827560 | 0.813885 | 2.040586e-51 | -8.615850e-47 |
| Msa0661040 | Msa0829770 | 0.808125 | 3.612197e-50 | -8.615850e-47 |
| Msa0661040 | Msa0862750 | 0.821956 | 3.064960e-53 | -8.615850e-47 |
| Msa0661040 | Msa0869320 | 0.808372 | 3.199613e-50 | -8.615850e-47 |
| Msa0661040 | Msa0870220 | 0.843060 | 1.767407e-58 | -8.615850e-47 |
| Msa0661040 | Msa0872890 | 0.801199 | 1.007931e-48 | -8.615850e-47 |
| Msa0661040 | Msa0876150 | 0.816269 | 6.032655e-52 | -8.615850e-47 |
| Msa0661040 | Msa0878090 | 0.800034 | 1.741899e-48 | -8.615850e-47 |
| Msa0661040 | Msa0898830 | 0.872516 | 2.996203e-67 | -8.615850e-47 |
| Msa0661040 | Msa0903460 | 0.811540 | 6.651828e-51 | -8.615850e-47 |
| Msa0661040 | Msa0906100 | 0.816510 | 5.328924e-52 | -8.615850e-47 |
| Msa0661040 | Msa0930090 | 0.806832 | 6.790862e-50 | -8.615850e-47 |
| Msa0661040 | Msa0934290 | 0.823614 | 1.259879e-53 | -8.615850e-47 |
| Msa0661040 | Msa0935930 | 0.852245 | 5.237572e-61 | -8.615850e-47 |
| Msa0661040 | Msa0943870 | 0.826648 | 2.417066e-54 | -8.615850e-47 |
| Msa0661040 | Msa0956600 | 0.806596 | 7.619587e-50 | -8.615850e-47 |
| Msa0661040 | Msa0964150 | 0.801393 | 9.197104e-49 | -8.615850e-47 |
| Msa0661040 | Msa0971780 | 0.821787 | 3.352377e-53 | -8.615850e-47 |
| Msa0661040 | Msa0972930 | 0.843157 | 1.664962e-58 | -8.615850e-47 |
| Msa0661040 | Msa0993590 | 0.806952 | 6.406362e-50 | -8.615850e-47 |
| Msa0661040 | Msa1009150 | 0.806483 | 8.048757e-50 | -8.615850e-47 |
| Msa0661040 | Msa1009170 | 0.815330 | 9.767992e-52 | -8.615850e-47 |
| Msa0661040 | Msa1016450 | 0.811813 | 5.803186e-51 | -8.615850e-47 |
| Msa0661040 | Msa1018210 | 0.849721 | 2.695755e-60 | -8.615850e-47 |
| Msa0661040 | Msa1029570 | 0.808707 | 2.713721e-50 | -8.615850e-47 |
| Msa0661040 | Msa1038570 | 0.802842 | 4.630444e-49 | -8.615850e-47 |
| Msa0661040 | Msa1055710 | 0.800767 | 1.235017e-48 | -8.615850e-47 |
| Msa0661040 | Msa1057940 | 0.840318 | 9.343830e-58 | -8.615850e-47 |
| Msa0661040 | Msa1084340 | 0.836390 | 9.622104e-57 | -8.615850e-47 |
| Msa0661040 | Msa1090020 | 0.816024 | 6.843094e-52 | -8.615850e-47 |
| Msa0661040 | Msa1098350 | 0.805413 | 1.351244e-49 | -8.615850e-47 |
| Msa0661040 | Msa1109500 | 0.801982 | 6.962075e-49 | -8.615850e-47 |
| Msa0661040 | Msa1109880 | -0.806205 | 9.208431e-50 | -8.615850e-47 |
| Msa0661040 | Msa1116850 | 0.854632 | 1.081705e-61 | -8.615850e-47 |
| Msa0661040 | Msa1121740 | 0.823922 | 1.067143e-53 | -8.615850e-47 |
| Msa0661040 | Msa1138490 | 0.801228 | 9.940561e-49 | -8.615850e-47 |
| Msa0661040 | Msa1143060 | 0.809554 | 1.786839e-50 | -8.615850e-47 |
| Msa0661040 | Msa1144380 | 0.802768 | 4.797330e-49 | -8.615850e-47 |
| Msa0661040 | Msa1145930 | 0.871996 | 4.462963e-67 | -8.615850e-47 |
| Msa0661040 | Msa1152860 | -0.816831 | 4.513948e-52 | -8.615850e-47 |
| Msa0661040 | Msa1164830 | 0.818755 | 1.660933e-52 | -8.615850e-47 |
| Msa0661040 | Msa1194480 | 0.811470 | 6.890580e-51 | -8.615850e-47 |
| Msa0661040 | Msa1199540 | 0.801416 | 9.098020e-49 | -8.615850e-47 |
| Msa0661040 | Msa1203090 | 0.807395 | 5.160692e-50 | -8.615850e-47 |
| Msa0661040 | Msa1278080 | 0.806077 | 9.800990e-50 | -8.615850e-47 |
| Msa0661040 | Msa1279310 | 0.807898 | 4.036782e-50 | -8.615850e-47 |
| Msa0661040 | Msa1313840 | -0.802621 | 5.142991e-49 | -8.615850e-47 |
| Msa0661040 | Msa1320550 | 0.829470 | 5.056797e-55 | -8.615850e-47 |
| Msa0661040 | Msa1323120 | -0.805317 | 1.414872e-49 | -8.615850e-47 |
| Msa0661040 | Msa1327690 | 0.806725 | 7.153792e-50 | -8.615850e-47 |
| Msa0661040 | Msa1341390 | 0.827897 | 1.213503e-54 | -8.615850e-47 |
| Msa0661040 | Msa1348860 | 0.816701 | 4.829679e-52 | -8.615850e-47 |
| Msa0661040 | Msa1369220 | 0.808501 | 3.002034e-50 | -8.615850e-47 |
| Msa0661040 | Msa1374210 | 0.808231 | 3.429053e-50 | -8.615850e-47 |
| Msa0661040 | Msa1378840 | 0.801750 | 7.769972e-49 | -8.615850e-47 |
| Msa0661040 | Msa1384410 | 0.814700 | 1.347995e-51 | -8.615850e-47 |
| Msa0661040 | Msa1384970 | 0.819015 | 1.449788e-52 | -8.615850e-47 |
| Msa0661040 | Msa1390480 | 0.806650 | 7.421593e-50 | -8.615850e-47 |
| Msa0661040 | Msa1395360 | 0.803875 | 2.828731e-49 | -8.615850e-47 |
| Msa0661040 | Msa1408900 | 0.817334 | 3.480148e-52 | -8.615850e-47 |
| Msa0661040 | Msa1414560 | 0.823539 | 1.311797e-53 | -8.615850e-47 |
| Msa0661040 | Msa1422150 | 0.807107 | 5.941633e-50 | -8.615850e-47 |
| Msa0661040 | Msa1443440 | 0.807680 | 4.490403e-50 | -8.615850e-47 |
| Msa0661040 | Msa1454330 | 0.800598 | 1.336836e-48 | -8.615850e-47 |
| Msa0661040 | Msa1464470 | 0.840289 | 9.510067e-58 | -8.615850e-47 |
| Msa0121460 | Msa0661040 | 0.812259 | 4.638965e-51 | -8.615850e-47 |
| Msa0127220 | Msa0661040 | 0.801847 | 7.422178e-49 | -8.615850e-47 |
| Msa0127910 | Msa0661040 | 0.804289 | 2.320706e-49 | -8.615850e-47 |
| Msa0148000 | Msa0661040 | 0.829843 | 4.101722e-55 | -8.615850e-47 |
| Msa0154720 | Msa0661040 | 0.814988 | 1.164032e-51 | -8.615850e-47 |
| Msa0163340 | Msa0661040 | -0.806730 | 7.136822e-50 | -8.615850e-47 |
| Msa0174060 | Msa0661040 | 0.818637 | 1.766615e-52 | -8.615850e-47 |
| Msa0183960 | Msa0661040 | 0.813636 | 2.315705e-51 | -8.615850e-47 |
| Msa0189150 | Msa0661040 | 0.818727 | 1.685397e-52 | -8.615850e-47 |
| Msa0198140 | Msa0661040 | 0.833619 | 4.806811e-56 | -8.615850e-47 |
| Msa0198220 | Msa0661040 | 0.825855 | 3.732814e-54 | -8.615850e-47 |
| Msa0212490 | Msa0661040 | -0.832794 | 7.713240e-56 | -8.615850e-47 |
| Msa0225070 | Msa0661040 | 0.800848 | 1.188867e-48 | -8.615850e-47 |
| Msa0228360 | Msa0661040 | 0.810152 | 1.328477e-50 | -8.615850e-47 |
| Msa0232660 | Msa0661040 | 0.805911 | 1.062109e-49 | -8.615850e-47 |
| Msa0384330 | Msa0661040 | 0.848720 | 5.120253e-60 | -8.615850e-47 |
| Msa0390590 | Msa0661040 | 0.802235 | 6.175894e-49 | -8.615850e-47 |
| Msa0403740 | Msa0661040 | 0.808636 | 2.809618e-50 | -8.615850e-47 |
| Msa0408160 | Msa0661040 | 0.808265 | 3.371048e-50 | -8.615850e-47 |
| Msa0409000 | Msa0661040 | 0.807305 | 5.392611e-50 | -8.615850e-47 |
| Msa0410960 | Msa0661040 | 0.846415 | 2.203213e-59 | -8.615850e-47 |
| Msa0422230 | Msa0661040 | 0.812024 | 5.219857e-51 | -8.615850e-47 |
| Msa0427260 | Msa0661040 | 0.816014 | 6.879196e-52 | -8.615850e-47 |
| Msa0430320 | Msa0661040 | -0.805801 | 1.120150e-49 | -8.615850e-47 |
| Msa0432260 | Msa0661040 | 0.805737 | 1.155233e-49 | -8.615850e-47 |
| Msa0445830 | Msa0661040 | 0.810165 | 1.320146e-50 | -8.615850e-47 |
| Msa0450430 | Msa0661040 | 0.819718 | 1.002682e-52 | -8.615850e-47 |
| Msa0451760 | Msa0661040 | 0.818858 | 1.573782e-52 | -8.615850e-47 |
| Msa0452120 | Msa0661040 | 0.819313 | 1.240025e-52 | -8.615850e-47 |
| Msa0452230 | Msa0661040 | 0.822449 | 2.354504e-53 | -8.615850e-47 |
| Msa0452490 | Msa0661040 | 0.832058 | 1.173563e-55 | -8.615850e-47 |
| Msa0478660 | Msa0661040 | 0.817780 | 2.761701e-52 | -8.615850e-47 |
| Msa0252880 | Msa0661040 | -0.833315 | 5.722454e-56 | -8.615850e-47 |
| Msa0258280 | Msa0661040 | 0.802617 | 5.153013e-49 | -8.615850e-47 |
| Msa0270730 | Msa0661040 | 0.804378 | 2.223257e-49 | -8.615850e-47 |
| Msa0276610 | Msa0661040 | 0.829898 | 3.977558e-55 | -8.615850e-47 |
| Msa0278340 | Msa0661040 | 0.830803 | 2.390278e-55 | -8.615850e-47 |
| Msa0279460 | Msa0661040 | 0.804596 | 2.002494e-49 | -8.615850e-47 |
| Msa0290800 | Msa0661040 | -0.829652 | 4.565753e-55 | -8.615850e-47 |
| Msa0291090 | Msa0661040 | -0.824226 | 9.051724e-54 | -8.615850e-47 |
| Msa0304350 | Msa0661040 | 0.816840 | 4.493417e-52 | -8.615850e-47 |
| Msa0305910 | Msa0661040 | 0.817189 | 3.751241e-52 | -8.615850e-47 |
| Msa0314690 | Msa0661040 | 0.801659 | 8.111437e-49 | -8.615850e-47 |
| Msa0315010 | Msa0661040 | 0.812436 | 4.243228e-51 | -8.615850e-47 |
| Msa0323790 | Msa0661040 | 0.801218 | 9.987287e-49 | -8.615850e-47 |
| Msa0328870 | Msa0661040 | -0.818919 | 1.524797e-52 | -8.615850e-47 |
| Msa0329650 | Msa0661040 | -0.837397 | 5.326333e-57 | -8.615850e-47 |
| Msa0330770 | Msa0661040 | 0.811418 | 7.072911e-51 | -8.615850e-47 |
| Msa0340830 | Msa0661040 | 0.805631 | 1.216190e-49 | -8.615850e-47 |
| Msa0343230 | Msa0661040 | 0.814377 | 1.589209e-51 | -8.615850e-47 |
| Msa0351930 | Msa0661040 | 0.832956 | 7.031393e-56 | -8.615850e-47 |
| Msa0355630 | Msa0661040 | 0.811141 | 8.120888e-51 | -8.615850e-47 |
| Msa0364030 | Msa0661040 | 0.808222 | 3.443173e-50 | -8.615850e-47 |
| Msa0482760 | Msa0661040 | -0.804081 | 2.562995e-49 | -8.615850e-47 |
| Msa0503620 | Msa0661040 | 0.852024 | 6.055224e-61 | -8.615850e-47 |
| Msa0510660 | Msa0661040 | 0.805871 | 1.082972e-49 | -8.615850e-47 |
| Msa0522730 | Msa0661040 | 0.836555 | 8.735061e-57 | -8.615850e-47 |
| Msa0526840 | Msa0661040 | 0.807870 | 4.092344e-50 | -8.615850e-47 |
| Msa0533140 | Msa0661040 | 0.802087 | 6.625097e-49 | -8.615850e-47 |
| Msa0533610 | Msa0661040 | 0.827630 | 1.406658e-54 | -8.615850e-47 |
| Msa0540160 | Msa0661040 | 0.849706 | 2.722076e-60 | -8.615850e-47 |
| Msa0545750 | Msa0661040 | 0.818359 | 2.043059e-52 | -8.615850e-47 |
| Msa0559440 | Msa0661040 | 0.828593 | 8.249290e-55 | -8.615850e-47 |
| Msa0559640 | Msa0661040 | 0.811532 | 6.678955e-51 | -8.615850e-47 |
| Msa0564580 | Msa0661040 | 0.835142 | 1.993684e-56 | -8.615850e-47 |
| Msa0565150 | Msa0661040 | 0.803230 | 3.848636e-49 | -8.615850e-47 |
| Msa0570250 | Msa0661040 | 0.828918 | 6.881931e-55 | -8.615850e-47 |
| Msa0572730 | Msa0661040 | 0.805401 | 1.359046e-49 | -8.615850e-47 |
| Msa0577960 | Msa0661040 | 0.815363 | 9.605079e-52 | -8.615850e-47 |
| Msa0578890 | Msa0661040 | 0.802434 | 5.621404e-49 | -8.615850e-47 |
| Msa0582270 | Msa0661040 | 0.800097 | 1.691126e-48 | -8.615850e-47 |
| Msa0591490 | Msa0661040 | -0.803907 | 2.785565e-49 | -8.615850e-47 |
| Msa0593600 | Msa0661040 | -0.800465 | 1.423081e-48 | -8.615850e-47 |
| Msa0601470 | Msa0661040 | 0.822328 | 2.511722e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0661040 | MtrunA17_Chr4g0032291 | 55.462 | 119 | 4 | 1 | 43 | 112 | 46 | 164 | 2.34e-34 | 116 |
| Msa0661040 | MtrunA17_Chr4g0032571 | 49.275 | 138 | 21 | 2 | 12 | 101 | 1 | 137 | 1.20e-27 | 98.6 |
| Msa0661040 | MtrunA17_Chr4g0032581 | 50.000 | 148 | 22 | 4 | 14 | 112 | 8 | 152 | 4.27e-27 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0661040 | AT2G26975.1 | 36.735 | 147 | 38 | 4 | 12 | 107 | 1 | 143 | 5.15e-18 | 74.3 |
| Msa0661040 | AT5G59030.1 | 32.500 | 120 | 29 | 2 | 43 | 110 | 45 | 164 | 4.63e-11 | 57.0 |
| Msa0661040 | AT3G46900.1 | 32.203 | 118 | 29 | 3 | 44 | 110 | 34 | 151 | 6.32e-11 | 56.2 |
Find 40 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGGTTGGGTTAGCTTATT+TGG | 0.161485 | 4_3:+72248172 | Msa0661040:intron |
| ACCCATCATCATGGTGTTAT+TGG | 0.242191 | 4_3:-72247928 | None:intergenic |
| AGAGGTTACTGTGTGGTTAC+AGG | 0.278616 | 4_3:-72247814 | None:intergenic |
| TCTTATGTAGCAGTGCTATT+AGG | 0.289363 | 4_3:+72248267 | Msa0661040:CDS |
| TGATGCACATGACATTCTTC+TGG | 0.300847 | 4_3:+72247967 | Msa0661040:CDS |
| ATGTAATAAATGTGTATTTA+GGG | 0.312526 | 4_3:-72247782 | None:intergenic |
| TAAGCCTTATCATCATGTTT+TGG | 0.336110 | 4_3:-72248293 | None:intergenic |
| AATGTAATAAATGTGTATTT+AGG | 0.339646 | 4_3:-72247783 | None:intergenic |
| TACTGTGTGGTTACAGGGTT+TGG | 0.340111 | 4_3:-72247808 | None:intergenic |
| CTTTAATTCTCTTCAACAAT+TGG | 0.349322 | 4_3:+72248000 | Msa0661040:CDS |
| GCTGTGCTTGGTCATGCTCT+TGG | 0.350764 | 4_3:+72248239 | Msa0661040:CDS |
| TTGGCTCTTATGTCTTTCAA+TGG | 0.387989 | 4_3:+72248200 | Msa0661040:CDS |
| GTTAGCTTATTTGGTTATGT+TGG | 0.388968 | 4_3:+72248181 | Msa0661040:intron |
| GAAACCAAAACATGATGATA+AGG | 0.457740 | 4_3:+72248289 | Msa0661040:CDS |
| GATGCACATGACATTCTTCT+GGG | 0.472072 | 4_3:+72247968 | Msa0661040:CDS |
| AACCAATAACACCATGATGA+TGG | 0.477184 | 4_3:+72247926 | Msa0661040:CDS |
| CTCTTCAACAATTGGCCTAA+CGG | 0.488088 | 4_3:+72248008 | Msa0661040:CDS |
| ATGCACGGTGGCGGCATGGA+TGG | 0.492115 | 4_3:+72247870 | Msa0661040:CDS |
| AATTGGCCTAACGGTGATAC+CGG | 0.503435 | 4_3:+72248017 | Msa0661040:CDS |
| GATGATGATGATGGTGTTGG+TGG | 0.515817 | 4_3:-72247903 | None:intergenic |
| TTATTGGTTGATGATGATGA+TGG | 0.522036 | 4_3:-72247912 | None:intergenic |
| CTCTTCAGATCTCACATCCA+TGG | 0.529767 | 4_3:+72247833 | Msa0661040:exon |
| GAGGTTACTGTGTGGTTACA+GGG | 0.531390 | 4_3:-72247813 | None:intergenic |
| ACCAATAACACCATGATGAT+GGG | 0.534019 | 4_3:+72247927 | Msa0661040:CDS |
| GGTGGTAACATGCACGGTGG+CGG | 0.534398 | 4_3:+72247861 | Msa0661040:CDS |
| GCTCTTATGTCTTTCAATGG+TGG | 0.539076 | 4_3:+72248203 | Msa0661040:CDS |
| GATCTCACATCCATGGATCA+TGG | 0.541471 | 4_3:+72247840 | Msa0661040:exon |
| GTTGATGATGATGATGGTGT+TGG | 0.554195 | 4_3:-72247906 | None:intergenic |
| CATGTTACCACCATGATCCA+TGG | 0.570126 | 4_3:-72247850 | None:intergenic |
| CATCTTATGACCCATCATCA+TGG | 0.576922 | 4_3:-72247937 | None:intergenic |
| CATGGATGTGAGATCTGAAG+AGG | 0.588461 | 4_3:-72247832 | None:intergenic |
| TAACATGCACGGTGGCGGCA+TGG | 0.592169 | 4_3:+72247866 | Msa0661040:CDS |
| ATGATGGGTCATAAGATGCA+CGG | 0.642320 | 4_3:+72247942 | Msa0661040:CDS |
| AACAATTAACAAGACAAGGG+AGG | 0.655863 | 4_3:-72248323 | None:intergenic |
| TACATACCGGTATCACCGTT+AGG | 0.669036 | 4_3:-72248023 | None:intergenic |
| ATGCACATGACATTCTTCTG+GGG | 0.701142 | 4_3:+72247969 | Msa0661040:CDS |
| GATCATGGTGGTAACATGCA+CGG | 0.714076 | 4_3:+72247855 | Msa0661040:CDS |
| CTCACATCCATGGATCATGG+TGG | 0.733160 | 4_3:+72247843 | Msa0661040:exon |
| CATGGTGGTAACATGCACGG+TGG | 0.787274 | 4_3:+72247858 | Msa0661040:CDS |
| GATCTGAAGAGGTTACTGTG+TGG | 0.856147 | 4_3:-72247821 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTAATAAATGTGTATTTA+GGG | - | chr4_3:72247785-72247804 | None:intergenic | 15.0% |
| !! | AATGTAATAAATGTGTATTT+AGG | - | chr4_3:72247786-72247805 | None:intergenic | 15.0% |
| ! | CTTTAATTCTCTTCAACAAT+TGG | + | chr4_3:72248000-72248019 | Msa0661040:CDS | 25.0% |
| !! | TTATTGGTTGATGATGATGA+TGG | - | chr4_3:72247915-72247934 | None:intergenic | 30.0% |
| ! | GTTAGCTTATTTGGTTATGT+TGG | + | chr4_3:72248181-72248200 | Msa0661040:intron | 30.0% |
| GAAACCAAAACATGATGATA+AGG | + | chr4_3:72248289-72248308 | Msa0661040:CDS | 30.0% | |
| !! | TAAGCCTTATCATCATGTTT+TGG | - | chr4_3:72248296-72248315 | None:intergenic | 30.0% |
| AACCAATAACACCATGATGA+TGG | + | chr4_3:72247926-72247945 | Msa0661040:CDS | 35.0% | |
| ACCAATAACACCATGATGAT+GGG | + | chr4_3:72247927-72247946 | Msa0661040:CDS | 35.0% | |
| TGATACCGGTATGTATGTAT+TGG | + | chr4_3:72248031-72248050 | Msa0661040:intron | 35.0% | |
| TTGAATTACTCTCACGTACA+CGG | + | chr4_3:72248087-72248106 | Msa0661040:intron | 35.0% | |
| !! | CAAAAGTGTCTGAAACAAAC+CGG | - | chr4_3:72248144-72248163 | None:intergenic | 35.0% |
| ! | AGACACTTTTGCATGTTCTT+AGG | + | chr4_3:72248153-72248172 | Msa0661040:intron | 35.0% |
| ! | GACACTTTTGCATGTTCTTA+GGG | + | chr4_3:72248154-72248173 | Msa0661040:intron | 35.0% |
| ! | TTTTGCATGTTCTTAGGGTT+GGG | + | chr4_3:72248159-72248178 | Msa0661040:intron | 35.0% |
| ! | TTGGCTCTTATGTCTTTCAA+TGG | + | chr4_3:72248200-72248219 | Msa0661040:CDS | 35.0% |
| !! | TTTCAATGGTGGTGTGTTTT+TGG | + | chr4_3:72248214-72248233 | Msa0661040:CDS | 35.0% |
| ! | TCTTATGTAGCAGTGCTATT+AGG | + | chr4_3:72248267-72248286 | Msa0661040:CDS | 35.0% |
| !! | GTTGATGATGATGATGGTGT+TGG | - | chr4_3:72247909-72247928 | None:intergenic | 40.0% |
| !! | ACCCATCATCATGGTGTTAT+TGG | - | chr4_3:72247931-72247950 | None:intergenic | 40.0% |
| CATCTTATGACCCATCATCA+TGG | - | chr4_3:72247940-72247959 | None:intergenic | 40.0% | |
| !! | ATGATGGGTCATAAGATGCA+CGG | + | chr4_3:72247942-72247961 | Msa0661040:CDS | 40.0% |
| TGATGCACATGACATTCTTC+TGG | + | chr4_3:72247967-72247986 | Msa0661040:CDS | 40.0% | |
| GATGCACATGACATTCTTCT+GGG | + | chr4_3:72247968-72247987 | Msa0661040:CDS | 40.0% | |
| ATGCACATGACATTCTTCTG+GGG | + | chr4_3:72247969-72247988 | Msa0661040:CDS | 40.0% | |
| ! | CTCTTCAACAATTGGCCTAA+CGG | + | chr4_3:72248008-72248027 | Msa0661040:CDS | 40.0% |
| CAACGCCAATACATACATAC+CGG | - | chr4_3:72248039-72248058 | None:intergenic | 40.0% | |
| ! | GTACTGACATGACGAAAACA+AGG | - | chr4_3:72248064-72248083 | None:intergenic | 40.0% |
| ! | CTTTTGCATGTTCTTAGGGT+TGG | + | chr4_3:72248158-72248177 | Msa0661040:intron | 40.0% |
| !! | TAGGGTTGGGTTAGCTTATT+TGG | + | chr4_3:72248172-72248191 | Msa0661040:intron | 40.0% |
| GCTCTTATGTCTTTCAATGG+TGG | + | chr4_3:72248203-72248222 | Msa0661040:CDS | 40.0% | |
| TACTGTGTGGTTACAGGGTT+TGG | - | chr4_3:72247811-72247830 | None:intergenic | 45.0% | |
| GAGGTTACTGTGTGGTTACA+GGG | - | chr4_3:72247816-72247835 | None:intergenic | 45.0% | |
| AGAGGTTACTGTGTGGTTAC+AGG | - | chr4_3:72247817-72247836 | None:intergenic | 45.0% | |
| GATCTGAAGAGGTTACTGTG+TGG | - | chr4_3:72247824-72247843 | None:intergenic | 45.0% | |
| CATGGATGTGAGATCTGAAG+AGG | - | chr4_3:72247835-72247854 | None:intergenic | 45.0% | |
| CTCTTCAGATCTCACATCCA+TGG | + | chr4_3:72247833-72247852 | Msa0661040:exon | 45.0% | |
| GATCTCACATCCATGGATCA+TGG | + | chr4_3:72247840-72247859 | Msa0661040:exon | 45.0% | |
| CATGTTACCACCATGATCCA+TGG | - | chr4_3:72247853-72247872 | None:intergenic | 45.0% | |
| GATCATGGTGGTAACATGCA+CGG | + | chr4_3:72247855-72247874 | Msa0661040:CDS | 45.0% | |
| !! | GATGATGATGATGGTGTTGG+TGG | - | chr4_3:72247906-72247925 | None:intergenic | 45.0% |
| ! | AATTGGCCTAACGGTGATAC+CGG | + | chr4_3:72248017-72248036 | Msa0661040:CDS | 45.0% |
| TACATACCGGTATCACCGTT+AGG | - | chr4_3:72248026-72248045 | None:intergenic | 45.0% | |
| CGTACACGGTTCATTAAACC+CGG | + | chr4_3:72248101-72248120 | Msa0661040:intron | 45.0% | |
| !!! | GTGTTTTTGGTTGCTGTGCT+TGG | + | chr4_3:72248227-72248246 | Msa0661040:CDS | 45.0% |
| CTCACATCCATGGATCATGG+TGG | + | chr4_3:72247843-72247862 | Msa0661040:exon | 50.0% | |
| CATGGTGGTAACATGCACGG+TGG | + | chr4_3:72247858-72247877 | Msa0661040:CDS | 55.0% | |
| GAAACAAACCGGCAGCAACG+TGG | - | chr4_3:72248133-72248152 | None:intergenic | 55.0% | |
| GCTGTGCTTGGTCATGCTCT+TGG | + | chr4_3:72248239-72248258 | Msa0661040:CDS | 55.0% | |
| GGTGGTAACATGCACGGTGG+CGG | + | chr4_3:72247861-72247880 | Msa0661040:CDS | 60.0% | |
| ! | TAACATGCACGGTGGCGGCA+TGG | + | chr4_3:72247866-72247885 | Msa0661040:CDS | 60.0% |
| GCAGCAACGTGGTTCGAACC+GGG | - | chr4_3:72248122-72248141 | None:intergenic | 60.0% | |
| GGCAGCAACGTGGTTCGAAC+CGG | - | chr4_3:72248123-72248142 | None:intergenic | 60.0% | |
| GGTTCGAACCACGTTGCTGC+CGG | + | chr4_3:72248122-72248141 | Msa0661040:intron | 60.0% | |
| ! | ATGCACGGTGGCGGCATGGA+TGG | + | chr4_3:72247870-72247889 | Msa0661040:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 72247737 | 72248334 | 72247737 | ID=Msa0661040;Name=Msa0661040 |
| chr4_3 | mRNA | 72247737 | 72248334 | 72247737 | ID=Msa0661040-mRNA-1;Parent=Msa0661040;Name=Msa0661040-mRNA-1;_AED=0.29;_eAED=0.29;_QI=115|0|0.5|1|0|0|2|0|112 |
| chr4_3 | exon | 72247737 | 72248038 | 72247737 | ID=Msa0661040-mRNA-1:exon:21644;Parent=Msa0661040-mRNA-1 |
| chr4_3 | exon | 72248186 | 72248334 | 72248186 | ID=Msa0661040-mRNA-1:exon:21645;Parent=Msa0661040-mRNA-1 |
| chr4_3 | five_prime_UTR | 72247737 | 72247851 | 72247737 | ID=Msa0661040-mRNA-1:five_prime_utr;Parent=Msa0661040-mRNA-1 |
| chr4_3 | CDS | 72247852 | 72248038 | 72247852 | ID=Msa0661040-mRNA-1:cds;Parent=Msa0661040-mRNA-1 |
| chr4_3 | CDS | 72248186 | 72248334 | 72248186 | ID=Msa0661040-mRNA-1:cds;Parent=Msa0661040-mRNA-1 |
| Gene Sequence |
| Protein sequence |
