AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0533610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0533610 MtrunA17_Chr4g0007171 77.852 149 33 0 1 149 1 149 1.96e-81 238
Msa0533610 MtrunA17_Chr4g0007101 76.774 155 36 0 1 155 1 155 7.95e-78 250
Msa0533610 MtrunA17_Chr4g0007331 79.195 149 31 0 1 149 1 149 1.62e-75 244
Msa0533610 MtrunA17_Chr4g0007201 77.181 149 34 0 1 149 1 149 4.51e-75 243
Msa0533610 MtrunA17_Chr4g0007131 75.168 149 37 0 1 149 1 149 8.58e-75 236
Msa0533610 MtrunA17_Chr5g0419951 70.968 155 42 1 1 155 1 152 1.76e-73 225
Msa0533610 MtrunA17_Chr4g0007211 75.168 149 37 0 1 149 1 149 4.85e-73 237
Msa0533610 MtrunA17_Chr4g0007151 71.515 165 44 1 1 162 1 165 5.19e-73 237
Msa0533610 MtrunA17_Chr4g0007091 74.497 149 32 1 1 149 1 143 2.53e-68 224
Msa0533610 MtrunA17_Chr4g0007431 50.993 151 67 4 1 149 1 146 7.49e-36 131
Msa0533610 MtrunA17_Chr4g0008211 45.860 157 82 3 1 155 1 156 2.16e-35 130
Msa0533610 MtrunA17_Chr4g0007481 45.570 158 82 4 1 155 1 157 2.45e-35 124
Msa0533610 MtrunA17_Chr4g0005021 51.773 141 66 2 10 149 8 147 2.58e-35 130
Msa0533610 MtrunA17_Chr4g0007461 45.570 158 82 4 1 155 1 157 2.11e-33 123
Msa0533610 MtrunA17_Chr4g0007521 45.570 158 82 4 1 155 1 157 5.65e-33 123
Msa0533610 MtrunA17_Chr4g0007511 45.570 158 82 4 1 155 1 157 7.47e-33 122
Msa0533610 MtrunA17_Chr4g0007451 45.223 157 83 3 1 155 1 156 2.02e-32 121
Msa0533610 MtrunA17_Chr4g0007381 49.359 156 77 2 1 155 1 155 4.33e-32 120
Msa0533610 MtrunA17_Chr4g0008271 49.645 141 68 3 17 155 19 158 4.71e-30 114
Msa0533610 MtrunA17_Chr4g0008291 46.259 147 73 3 10 155 5 146 2.92e-29 112
Msa0533610 MtrunA17_Chr4g0007571 51.181 127 60 2 30 155 20 145 2.84e-28 109
Msa0533610 MtrunA17_Chr2g0316441 38.182 165 78 4 14 155 12 175 1.18e-22 93.6
Msa0533610 MtrunA17_Chr7g0216801 35.714 154 88 5 5 149 29 180 2.23e-21 90.1
Msa0533610 MtrunA17_Chr5g0439791 36.242 149 80 3 30 163 26 174 2.48e-20 87.0
Msa0533610 MtrunA17_Chr5g0440141 39.189 148 73 5 30 163 32 176 2.60e-20 87.0
Msa0533610 MtrunA17_Chr7g0216641 36.957 138 77 4 30 158 35 171 9.35e-20 85.5
Msa0533610 MtrunA17_Chr4g0024361 38.356 146 77 5 29 162 31 175 1.25e-19 85.1
Msa0533610 MtrunA17_Chr3g0093661 37.584 149 69 4 30 155 31 178 1.82e-19 84.3
Msa0533610 MtrunA17_Chr5g0440201 38.961 154 71 6 30 163 28 178 1.90e-19 84.3
Msa0533610 MtrunA17_Chr5g0426041 36.810 163 86 6 8 155 3 163 2.29e-19 84.3
Msa0533610 MtrunA17_Chr5g0439921 40.299 134 65 4 30 149 32 164 2.37e-19 84.3
Msa0533610 MtrunA17_Chr4g0032101 33.136 169 94 5 1 155 7 170 3.52e-19 83.6
Msa0533610 MtrunA17_Chr5g0440161 36.310 168 84 5 10 155 4 170 3.53e-19 83.6
Msa0533610 MtrunA17_Chr5g0439901 38.571 140 71 4 30 155 32 170 3.55e-19 82.8
Msa0533610 MtrunA17_Chr7g0216731 35.507 138 83 4 26 158 38 174 6.91e-19 82.8
Msa0533610 MtrunA17_Chr5g0440011 39.865 148 70 6 30 163 32 174 1.51e-18 82.0
Msa0533610 MtrunA17_Chr5g0440101 37.584 149 75 5 30 163 32 177 1.58e-18 82.0
Msa0533610 MtrunA17_Chr4g0007561 35.583 163 93 6 1 155 53 211 1.82e-18 80.1
Msa0533610 MtrunA17_Chr5g0440031 38.514 148 74 5 30 163 33 177 1.97e-18 81.6
Msa0533610 MtrunA17_Chr4g0007441 35.583 163 93 6 1 155 53 211 3.11e-18 80.9
Msa0533610 MtrunA17_Chr5g0446891 35.664 143 83 5 30 164 66 207 3.28e-18 80.9
Msa0533610 MtrunA17_Chr7g0216941 32.099 162 101 4 5 158 4 164 5.89e-18 80.1
Msa0533610 MtrunA17_Chr7g0216771 34.507 142 83 4 26 158 31 171 1.04e-17 79.3
Msa0533610 MtrunA17_Chr7g0216701 35.507 138 79 4 30 158 35 171 3.22e-17 78.2
Msa0533610 MtrunA17_Chr2g0293591 40.000 115 63 4 54 163 42 155 3.51e-17 77.8
Msa0533610 MtrunA17_Chr3g0087631 36.242 149 77 6 30 163 26 171 4.02e-17 77.8
Msa0533610 MtrunA17_Chr7g0217371 35.971 139 79 4 30 159 35 172 4.14e-17 77.8
Msa0533610 MtrunA17_Chr5g0440151 37.313 134 69 4 30 149 32 164 4.78e-17 77.4
Msa0533610 MtrunA17_Chr5g0439821 37.671 146 70 5 30 155 26 170 5.11e-17 77.4
Msa0533610 MtrunA17_Chr7g0216791 34.058 138 81 4 30 158 13 149 1.39e-16 75.1
Msa0533610 MtrunA17_Chr1g0190571 31.544 149 89 6 9 149 4 147 7.01e-16 74.3
Msa0533610 MtrunA17_Chr5g0446201 32.877 146 86 5 30 164 63 207 1.20e-15 71.6
Msa0533610 MtrunA17_Chr4g0004161 29.814 161 83 4 17 149 8 166 1.52e-15 73.2
Msa0533610 MtrunA17_Chr7g0215761 32.836 134 80 4 25 149 25 157 1.82e-15 73.2
Msa0533610 MtrunA17_Chr0c03g0490031 34.483 145 88 5 6 149 4 142 6.30e-15 71.2
Msa0533610 MtrunA17_Chr7g0219371 34.194 155 92 6 1 149 1 151 2.41e-14 69.7
Msa0533610 MtrunA17_Chr5g0446871 37.500 136 75 6 30 156 26 160 2.62e-14 69.7
Msa0533610 MtrunA17_Chr4g0019841 32.386 176 80 7 16 159 23 191 3.82e-14 69.3
Msa0533610 MtrunA17_Chr5g0446021 38.710 93 54 2 62 151 64 156 8.84e-14 68.2
Msa0533610 MtrunA17_Chr5g0445731 40.860 93 52 2 60 149 62 154 9.03e-14 66.2
Msa0533610 MtrunA17_Chr1g0197051 42.857 91 48 2 58 146 46 134 1.71e-13 67.4
Msa0533610 MtrunA17_Chr1g0202001 36.066 122 67 5 27 142 69 185 2.18e-13 67.0
Msa0533610 MtrunA17_Chr8g0379101 39.823 113 64 2 38 150 37 145 2.81e-13 66.6
Msa0533610 MtrunA17_Chr7g0244931 38.144 97 56 2 54 148 56 150 2.96e-13 66.6
Msa0533610 MtrunA17_Chr2g0284741 30.556 180 99 8 3 163 5 177 3.75e-13 66.2
Msa0533610 MtrunA17_Chr3g0106171 33.735 166 98 6 1 161 2 160 3.97e-13 66.2
Msa0533610 MtrunA17_Chr4g0019861 35.185 162 80 9 18 163 19 171 4.57e-13 66.2
Msa0533610 MtrunA17_Chr1g0169841 35.246 122 68 3 34 149 35 151 4.69e-13 66.2
Msa0533610 MtrunA17_Chr7g0250471 33.108 148 89 6 9 153 6 146 8.62e-13 65.5
Msa0533610 MtrunA17_Chr5g0434551 32.456 114 75 1 45 158 33 144 1.05e-12 65.1
Msa0533610 MtrunA17_Chr8g0386631 31.579 133 85 3 25 155 19 147 1.23e-12 64.7
Msa0533610 MtrunA17_Chr4g0058861 32.680 153 92 7 1 149 1 146 1.35e-12 64.7
Msa0533610 MtrunA17_Chr8g0380811 39.831 118 58 6 32 142 34 145 1.70e-12 64.3
Msa0533610 MtrunA17_Chr6g0455791 33.913 115 70 3 45 155 112 224 2.47e-12 63.2
Msa0533610 MtrunA17_Chr6g0455801 34.426 122 71 4 30 149 31 145 3.13e-12 63.5
Msa0533610 MtrunA17_Chr1g0207531 33.813 139 86 3 12 149 8 141 3.52e-12 62.0
Msa0533610 MtrunA17_Chr5g0397651 33.136 169 98 6 7 166 3 165 6.16e-12 62.8
Msa0533610 MtrunA17_Chr6g0465181 36.923 130 63 6 30 146 39 162 6.26e-12 62.8
Msa0533610 MtrunA17_Chr6g0465141 35.507 138 64 7 30 149 33 163 6.33e-12 62.0
Msa0533610 MtrunA17_Chr8g0380831 44.186 86 39 4 64 142 65 148 7.45e-12 62.4
Msa0533610 MtrunA17_Chr2g0283061 37.113 97 58 2 54 149 15 109 8.08e-12 62.4
Msa0533610 MtrunA17_Chr5g0405191 39.316 117 65 4 34 149 27 138 9.00e-12 62.4
Msa0533610 MtrunA17_Chr5g0440211 36.792 106 61 3 58 159 51 154 1.04e-11 62.0
Msa0533610 MtrunA17_Chr1g0159161 34.259 108 63 3 53 157 39 141 1.08e-11 62.0
Msa0533610 MtrunA17_Chr4g0008241 36.923 130 65 7 24 142 25 148 1.51e-11 61.6
Msa0533610 MtrunA17_Chr4g0014151 37.255 102 59 3 60 156 63 164 1.56e-11 61.6
Msa0533610 MtrunA17_Chr2g0283981 40.594 101 58 1 55 155 52 150 1.77e-11 59.7
Msa0533610 MtrunA17_Chr5g0429421 32.298 161 91 7 4 155 11 162 2.33e-11 61.2
Msa0533610 MtrunA17_Chr2g0284161 40.217 92 51 2 66 155 70 159 2.79e-11 60.5
Msa0533610 MtrunA17_Chr6g0465131 36.800 125 63 5 30 142 39 159 4.36e-11 60.5
Msa0533610 MtrunA17_Chr8g0393211 29.545 132 83 3 31 161 34 156 5.48e-11 60.1
Msa0533610 MtrunA17_Chr6g0488781 33.083 133 79 5 23 151 23 149 5.92e-11 58.9
Msa0533610 MtrunA17_Chr4g0019801 32.704 159 78 8 18 156 19 168 8.30e-11 59.7
Msa0533610 MtrunA17_Chr8g0380951 32.639 144 85 5 3 140 5 142 9.42e-11 59.3
Msa0533610 MtrunA17_Chr8g0380941 32.639 144 85 5 3 140 5 142 9.55e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0533610 AT1G45616.1 37.063 143 72 4 30 155 36 177 8.62e-20 85.9
Msa0533610 AT3G28890.1 40.816 98 56 1 54 149 71 168 2.47e-17 78.6
Msa0533610 AT3G28890.2 40.816 98 56 1 54 149 71 168 2.47e-17 78.6
Msa0533610 AT4G13920.1 34.722 144 83 4 16 149 10 152 5.21e-17 77.8
Msa0533610 AT5G27060.2 36.090 133 71 3 30 149 38 169 6.07e-17 77.8
Msa0533610 AT5G27060.3 36.090 133 71 3 30 149 37 168 6.13e-17 77.8
Msa0533610 AT5G27060.1 36.090 133 71 3 30 149 37 168 6.13e-17 77.8
Msa0533610 AT4G18640.1 36.774 155 74 5 8 155 10 147 5.13e-16 75.1
Msa0533610 AT1G60800.1 33.803 142 80 4 5 146 15 142 6.50e-16 74.7
Msa0533610 AT1G71390.2 33.951 162 96 6 2 158 8 163 7.96e-16 74.3
Msa0533610 AT1G71390.1 33.951 162 96 6 2 158 3 158 8.02e-16 74.3
Msa0533610 AT4G13820.1 32.353 136 83 3 30 156 29 164 1.12e-15 73.9
Msa0533610 AT4G13880.1 32.099 162 98 5 2 155 8 165 1.74e-15 73.6
Msa0533610 AT2G15080.1 33.333 162 91 6 1 149 2 159 3.85e-15 72.4
Msa0533610 AT2G15080.2 33.333 162 91 6 1 149 2 159 3.85e-15 72.4
Msa0533610 AT2G42290.1 37.615 109 64 2 38 146 31 135 4.56e-15 72.0
Msa0533610 AT4G13810.2 31.944 144 94 2 16 155 19 162 1.11e-14 71.2
Msa0533610 AT3G57830.2 34.746 118 73 2 38 155 32 145 2.67e-14 69.7
Msa0533610 AT3G57830.3 35.484 124 74 3 38 159 32 151 3.62e-14 69.7
Msa0533610 AT3G57830.1 35.484 124 74 3 38 159 32 151 3.62e-14 69.7
Msa0533610 AT1G66830.1 40.816 98 55 2 53 149 42 137 7.13e-14 68.9
Msa0533610 AT1G47890.1 38.760 129 70 4 30 155 74 196 7.54e-14 68.9
Msa0533610 AT3G11010.2 34.091 132 74 4 30 149 38 168 8.56e-14 68.6
Msa0533610 AT3G11010.4 34.091 132 74 4 30 149 38 168 8.77e-14 68.6
Msa0533610 AT3G11010.3 34.091 132 74 4 30 149 38 168 9.13e-14 68.6
Msa0533610 AT2G01210.1 35.714 140 82 4 22 159 14 147 1.19e-13 68.2
Msa0533610 AT3G05360.1 31.333 150 98 4 3 149 11 158 1.63e-13 67.8
Msa0533610 AT2G07040.1 36.290 124 71 5 24 147 17 132 1.84e-13 67.4
Msa0533610 AT2G25790.1 38.462 117 64 6 32 144 29 141 4.51e-13 66.6
Msa0533610 AT3G11080.2 36.364 121 70 4 41 157 65 182 6.35e-13 66.2
Msa0533610 AT3G11080.1 36.364 121 70 4 41 157 65 182 6.35e-13 66.2
Msa0533610 AT1G71400.1 33.333 132 82 3 29 155 33 163 1.11e-12 65.5
Msa0533610 AT3G25560.2 38.202 89 53 1 55 143 60 146 1.15e-12 65.1
Msa0533610 AT3G25560.1 38.202 89 53 1 55 143 60 146 1.25e-12 65.1
Msa0533610 AT3G25560.3 38.202 89 53 1 55 143 60 146 1.29e-12 65.1
Msa0533610 AT3G02880.1 33.594 128 77 3 31 158 27 146 2.04e-12 64.7
Msa0533610 AT2G34930.1 35.461 141 71 7 30 155 33 168 7.22e-12 63.2
Msa0533610 AT3G05650.1 29.375 160 91 6 3 149 16 166 8.95e-12 62.8
Msa0533610 AT3G11010.5 38.889 90 53 1 62 149 16 105 1.07e-11 62.4
Msa0533610 AT3G11010.1 38.889 90 53 1 62 149 16 105 1.11e-11 62.4
Msa0533610 AT3G11010.6 38.889 90 53 1 62 149 16 105 1.14e-11 62.4
Msa0533610 AT5G65700.1 41.121 107 58 3 51 155 43 146 1.38e-11 62.4
Msa0533610 AT5G65700.2 41.121 107 58 3 51 155 43 146 1.38e-11 62.4
Msa0533610 AT3G24982.1 38.281 128 62 7 20 143 57 171 1.40e-11 62.0
Msa0533610 AT3G49670.1 40.566 106 58 3 52 155 44 146 1.60e-11 62.0
Msa0533610 AT2G33050.1 34.752 141 80 6 29 161 12 148 1.63e-11 62.0
Msa0533610 AT2G01820.1 36.264 91 56 1 54 144 342 430 1.67e-11 62.0
Msa0533610 AT5G63930.1 36.190 105 59 3 55 153 48 150 1.75e-11 62.0
Msa0533610 AT5G45840.2 38.095 105 60 3 55 156 52 154 1.76e-11 62.0
Msa0533610 AT5G45840.1 38.095 105 60 3 55 156 52 154 1.83e-11 61.6
Msa0533610 AT1G17250.1 32.800 125 73 5 25 145 47 164 2.02e-11 61.6
Msa0533610 AT3G47570.1 34.959 123 73 4 28 148 19 136 2.40e-11 61.6
Msa0533610 AT5G20480.1 35.833 120 71 4 29 146 26 141 2.67e-11 61.2
Msa0533610 AT5G20480.2 35.833 120 71 4 29 146 26 141 2.67e-11 61.2
Msa0533610 AT1G60800.2 40.789 76 43 1 71 146 2 75 2.84e-11 61.2
Msa0533610 AT1G63430.2 33.600 125 74 4 30 149 20 140 3.50e-11 60.8
Msa0533610 AT1G63430.3 33.600 125 74 4 30 149 20 140 3.94e-11 60.8
Msa0533610 AT1G63430.1 33.600 125 74 4 30 149 20 140 3.94e-11 60.8
Msa0533610 AT2G32680.1 45.833 72 39 0 78 149 172 243 4.12e-11 60.8
Msa0533610 AT1G48480.1 34.343 99 62 2 58 155 54 150 5.44e-11 60.5
Msa0533610 AT1G17240.1 31.967 122 72 5 28 145 44 158 6.46e-11 60.1
Msa0533610 AT1G17240.2 31.967 122 72 5 28 145 49 163 6.72e-11 60.1
Msa0533610 AT3G05660.1 32.317 164 94 7 5 157 6 163 7.02e-11 60.1

Find 30 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAGCACATATAATATTT+TGG 0.208144 4_1:+8679568 Msa0533610:CDS
TTATCTGCAGAGTCTGAATT+TGG 0.235114 4_1:+8679872 Msa0533610:CDS
CTTAGTCAAGAGTCTATTTC+CGG 0.293340 4_1:+8679813 Msa0533610:CDS
TTCTCCGATGCTGGCTTTGA+AGG 0.340579 4_1:+8679963 Msa0533610:CDS
AATCTTCCAAGCTAGTTCAT+TGG 0.376498 4_1:+8679718 Msa0533610:CDS
TTCATAAGCTGCAGAATTTG+AGG 0.387028 4_1:+8679931 Msa0533610:CDS
GAAAATATACATTTGACATC+AGG 0.414852 4_1:+8680017 Msa0533610:CDS
TATAATAATATTGTGGTGAA+TGG 0.440427 4_1:+8679627 Msa0533610:CDS
GGCAACAATCATTATCACTT+TGG 0.451944 4_1:-8679743 None:intergenic
TATTTGAACTTCTCCGATGC+TGG 0.455343 4_1:+8679954 Msa0533610:CDS
GGGTCAACAAGGAACGTTCA+TGG 0.470281 4_1:-8679662 None:intergenic
TAGACTCTTGACTAAGATCA+AGG 0.474967 4_1:-8679806 None:intergenic
TTGGTTCCAATGAACTAGCT+TGG 0.478994 4_1:-8679724 None:intergenic
CTTGTATATATTAAACTGAA+TGG 0.479985 4_1:-8679603 None:intergenic
TGCAGCTTATGAAGTTCTTG+TGG 0.487884 4_1:-8679921 None:intergenic
GATGGAGTAACATGCAAAGA+TGG 0.551500 4_1:+8679771 Msa0533610:CDS
AAAATATACATTTGACATCA+GGG 0.552047 4_1:+8680018 Msa0533610:CDS
TCAGACTCTGCAGATAATGC+AGG 0.559542 4_1:-8679866 None:intergenic
TGATAATGATTGTTGCCAAT+GGG 0.560593 4_1:+8679749 Msa0533610:CDS
ACTTGAATCATCAAGTCCTC+CGG 0.563338 4_1:-8679832 None:intergenic
TGCATGTTACTCCATCCCAT+TGG 0.563565 4_1:-8679764 None:intergenic
ATCAGTGGAGAAATATCTGA+AGG 0.567344 4_1:+8680048 Msa0533610:CDS
GTGATAATGATTGTTGCCAA+TGG 0.574199 4_1:+8679748 Msa0533610:CDS
CATCAGGGCTTTCACATCAG+TGG 0.584884 4_1:+8680033 Msa0533610:CDS
CTGTCCTTCAAAGCCAGCAT+CGG 0.589553 4_1:-8679967 None:intergenic
TACAAGCTATAATAATATTG+TGG 0.604958 4_1:+8679620 Msa0533610:CDS
AGACTCTTGACTAAGATCAA+GGG 0.618476 4_1:-8679805 None:intergenic
AGTCAAGAGTCTATTTCCGG+AGG 0.626705 4_1:+8679816 Msa0533610:CDS
AATGATTGTTGCCAATGGGA+TGG 0.636479 4_1:+8679753 Msa0533610:CDS
CTTTGAAGGACAGATCCCAA+AGG 0.685279 4_1:+8679977 Msa0533610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTGTATATATTAAACTGAA+TGG - chr4_1:8679606-8679625 None:intergenic 20.0%
!! TACAAGCTATAATAATATTG+TGG + chr4_1:8679620-8679639 Msa0533610:CDS 20.0%
!! TATAATAATATTGTGGTGAA+TGG + chr4_1:8679627-8679646 Msa0533610:CDS 20.0%
!! AAAATATACATTTGACATCA+GGG + chr4_1:8680018-8680037 Msa0533610:CDS 20.0%
!!! TGAGAGCACATATAATATTT+TGG + chr4_1:8679568-8679587 Msa0533610:CDS 25.0%
!!! TATGAGGTTGTTTTTGAAAT+GGG - chr4_1:8679685-8679704 None:intergenic 25.0%
!!! ATATGAGGTTGTTTTTGAAA+TGG - chr4_1:8679686-8679705 None:intergenic 25.0%
! GTAGATGAAAAATTTCCTTT+GGG - chr4_1:8679995-8680014 None:intergenic 25.0%
! AGTAGATGAAAAATTTCCTT+TGG - chr4_1:8679996-8680015 None:intergenic 25.0%
! GAAAATATACATTTGACATC+AGG + chr4_1:8680017-8680036 Msa0533610:CDS 25.0%
! TGATAATGATTGTTGCCAAT+GGG + chr4_1:8679749-8679768 Msa0533610:CDS 30.0%
!!! GTTTTTGAAATGGGTCAACA+AGG - chr4_1:8679676-8679695 None:intergenic 35.0%
! ATTTTGTGGGGTTGAATATG+AGG - chr4_1:8679701-8679720 None:intergenic 35.0%
!! ACTAGCTTGGAAGATTTTGT+GGG - chr4_1:8679714-8679733 None:intergenic 35.0%
!! AACTAGCTTGGAAGATTTTG+TGG - chr4_1:8679715-8679734 None:intergenic 35.0%
AATCTTCCAAGCTAGTTCAT+TGG + chr4_1:8679718-8679737 Msa0533610:CDS 35.0%
GGCAACAATCATTATCACTT+TGG - chr4_1:8679746-8679765 None:intergenic 35.0%
! GTGATAATGATTGTTGCCAA+TGG + chr4_1:8679748-8679767 Msa0533610:CDS 35.0%
AGACTCTTGACTAAGATCAA+GGG - chr4_1:8679808-8679827 None:intergenic 35.0%
TAGACTCTTGACTAAGATCA+AGG - chr4_1:8679809-8679828 None:intergenic 35.0%
CTTAGTCAAGAGTCTATTTC+CGG + chr4_1:8679813-8679832 Msa0533610:CDS 35.0%
TTATCTGCAGAGTCTGAATT+TGG + chr4_1:8679872-8679891 Msa0533610:CDS 35.0%
TTCATAAGCTGCAGAATTTG+AGG + chr4_1:8679931-8679950 Msa0533610:CDS 35.0%
! CTAGCTTGGAAGATTTTGTG+GGG - chr4_1:8679713-8679732 None:intergenic 40.0%
TTGGTTCCAATGAACTAGCT+TGG - chr4_1:8679727-8679746 None:intergenic 40.0%
AATGATTGTTGCCAATGGGA+TGG + chr4_1:8679753-8679772 Msa0533610:CDS 40.0%
GATGGAGTAACATGCAAAGA+TGG + chr4_1:8679771-8679790 Msa0533610:CDS 40.0%
ACTTGAATCATCAAGTCCTC+CGG - chr4_1:8679835-8679854 None:intergenic 40.0%
TGCAGCTTATGAAGTTCTTG+TGG - chr4_1:8679924-8679943 None:intergenic 40.0%
TATTTGAACTTCTCCGATGC+TGG + chr4_1:8679954-8679973 Msa0533610:CDS 40.0%
TGCATGTTACTCCATCCCAT+TGG - chr4_1:8679767-8679786 None:intergenic 45.0%
AGTCAAGAGTCTATTTCCGG+AGG + chr4_1:8679816-8679835 Msa0533610:CDS 45.0%
TCAGACTCTGCAGATAATGC+AGG - chr4_1:8679869-8679888 None:intergenic 45.0%
! CTTTGAAGGACAGATCCCAA+AGG + chr4_1:8679977-8679996 Msa0533610:CDS 45.0%
GGGTCAACAAGGAACGTTCA+TGG - chr4_1:8679665-8679684 None:intergenic 50.0%
!! TTCTCCGATGCTGGCTTTGA+AGG + chr4_1:8679963-8679982 Msa0533610:CDS 50.0%
CTGTCCTTCAAAGCCAGCAT+CGG - chr4_1:8679970-8679989 None:intergenic 50.0%
CATCAGGGCTTTCACATCAG+TGG + chr4_1:8680033-8680052 Msa0533610:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 8679567 8680067 8679567 ID=Msa0533610;Name=Msa0533610
chr4_1 mRNA 8679567 8680067 8679567 ID=Msa0533610-mRNA-1;Parent=Msa0533610;Name=Msa0533610-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|166
chr4_1 exon 8679567 8680067 8679567 ID=Msa0533610-mRNA-1:exon:2880;Parent=Msa0533610-mRNA-1
chr4_1 CDS 8679567 8680067 8679567 ID=Msa0533610-mRNA-1:cds;Parent=Msa0533610-mRNA-1
Gene Sequence

>Msa0533610

ATGAGAGCACATATAATATTTTGGTTTTTCTTTATACCATTCAGTTTAATATATACAAGCTATAATAATATTGTGGTGAATGGCTACTGTGATAGCCATGAACGTTCCTTGTTGACCCATTTCAAAAACAACCTCATATTCAACCCCACAAAATCTTCCAAGCTAGTTCATTGGAACCAAAGTGATAATGATTGTTGCCAATGGGATGGAGTAACATGCAAAGATGGATATGTTACAGCCCTTGATCTTAGTCAAGAGTCTATTTCCGGAGGACTTGATGATTCAAGTGCTCTTTTCAGCCTGCATTATCTGCAGAGTCTGAATTTGGCTTTTAATGACTTTCGTTCTACGATTCCACAAGAACTTCATAAGCTGCAGAATTTGAGGTATTTGAACTTCTCCGATGCTGGCTTTGAAGGACAGATCCCAAAGGAAATTTTTCATCTACTAGAAAATATACATTTGACATCAGGGCTTTCACATCAGTGGAGAAATATCTGA
Protein sequence

>Msa0533610

MRAHIIFWFFFIPFSLIYTSYNNIVVNGYCDSHERSLLTHFKNNLIFNPTKSSKLVHWNQSDNDCCQWDGVTCKDGYVTALDLSQESISGGLDDSSALFSLHYLQSLNLAFNDFRSTIPQELHKLQNLRYLNFSDAGFEGQIPKEIFHLLENIHLTSGLSHQWRNI*