AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0510660


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0510660 MtrunA17_Chr3g0116971 96.330 109 3 1 1 108 1 109 3.12e-71 207
Msa0510660 MtrunA17_Chr3g0116951 68.908 119 23 6 1 108 6 121 6.17e-42 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCCTCTCAACGATTTC+AGG 0.158944 3_4:-59798536 None:intergenic
TTGAAGGCCATCTCACCATT+TGG 0.332611 3_4:-59798449 None:intergenic
GGTTGTGGTGGTGGCGGCCA+TGG 0.367830 3_4:-59798514 None:intergenic
CCTTCGTGGGTAATGTTGAT+TGG 0.388716 3_4:-59798639 None:intergenic
CGATTTCAGGGTTGTGGTGG+TGG 0.397034 3_4:-59798523 None:intergenic
TGAAGGCCATCTCACCATTT+GGG 0.418823 3_4:-59798448 None:intergenic
CAACCCTCTCAACGATTTCA+GGG 0.425008 3_4:-59798535 None:intergenic
CCATTTGACCTGTTCCTTCG+TGG 0.432698 3_4:-59798653 None:intergenic
ATAGACAACCCTCTTGTTGA+AGG 0.438765 3_4:-59798465 None:intergenic
GTCACTCATGGAATACATTC+AGG 0.444691 3_4:+59798675 Msa0510660:CDS
CATTTGACCTGTTCCTTCGT+GGG 0.467646 3_4:-59798652 None:intergenic
CAACAAGAGGGTTGTCTATG+AGG 0.468986 3_4:+59798469 Msa0510660:CDS
CCCTTCGAACTTCTGAAATG+TGG 0.470828 3_4:-59798403 None:intergenic
TCTCAACGATTTCAGGGTTG+TGG 0.485866 3_4:-59798529 None:intergenic
TTTCAGGGTTGTGGTGGTGG+CGG 0.488542 3_4:-59798520 None:intergenic
CGTTGAGAGGGTTGAAGTGG+TGG 0.489360 3_4:+59798544 Msa0510660:CDS
AACATTACCCACGAAGGAAC+AGG 0.491085 3_4:+59798645 Msa0510660:CDS
TATGAGGAGGATGCGATTGA+AGG 0.492172 3_4:+59798485 Msa0510660:CDS
CCACGAAGGAACAGGTCAAA+TGG 0.498880 3_4:+59798653 Msa0510660:CDS
GTACCTGAGGTTAGGCCCTA+TGG 0.503566 3_4:+59798578 Msa0510660:CDS
CCACCATAGGGCCTAACCTC+AGG 0.504816 3_4:-59798581 None:intergenic
GAATACTCTCGCTACCCAAA+TGG 0.533741 3_4:+59798434 Msa0510660:CDS
CCTTCGAACTTCTGAAATGT+GGG 0.541962 3_4:-59798402 None:intergenic
ATGAACGTGTACCTGAGGTT+AGG 0.542173 3_4:+59798570 Msa0510660:CDS
CCCACATTTCAGAAGTTCGA+AGG 0.543003 3_4:+59798402 Msa0510660:CDS
ACAGGTCAAATGGTCACTCA+TGG 0.552434 3_4:+59798663 Msa0510660:CDS
CAACGATTTCAGGGTTGTGG+TGG 0.564097 3_4:-59798526 None:intergenic
GTAGATCACTTGACCACCAT+AGG 0.569439 3_4:-59798594 None:intergenic
AATGTGGGTGTGGTAGTCAT+TGG 0.581040 3_4:-59798387 None:intergenic
GGGTAGCGAGAGTATTCGCT+AGG 0.581864 3_4:-59798428 None:intergenic
AGTGATCTACGAAGAGGACG+TGG 0.585316 3_4:+59798607 Msa0510660:CDS
TCGCTACCCAAATGGTGAGA+TGG 0.585879 3_4:+59798442 Msa0510660:CDS
GTGAGATGGCCTTCAACAAG+AGG 0.602330 3_4:+59798456 Msa0510660:CDS
TAGATCACTTGACCACCATA+GGG 0.606041 3_4:-59798593 None:intergenic
GAACTTCTGAAATGTGGGTG+TGG 0.614497 3_4:-59798397 None:intergenic
CCACATTTCAGAAGTTCGAA+GGG 0.620439 3_4:+59798403 Msa0510660:CDS
CAACCCTGAAATCGTTGAGA+GGG 0.621410 3_4:+59798532 Msa0510660:CDS
CCTGAGGTTAGGCCCTATGG+TGG 0.626513 3_4:+59798581 Msa0510660:CDS
ACAACCCTGAAATCGTTGAG+AGG 0.647655 3_4:+59798531 Msa0510660:CDS
CAAGAGGGTTGTCTATGAGG+AGG 0.649483 3_4:+59798472 Msa0510660:CDS
GCGATTGAAGGATCACGCCA+TGG 0.655981 3_4:+59798497 Msa0510660:CDS
TGAGATGGCCTTCAACAAGA+GGG 0.673455 3_4:+59798457 Msa0510660:CDS
TGGTCAAGTGATCTACGAAG+AGG 0.694239 3_4:+59798601 Msa0510660:CDS
AATCGTTGAGAGGGTTGAAG+TGG 0.698905 3_4:+59798541 Msa0510660:CDS
CCAATCAACATTACCCACGA+AGG 0.702298 3_4:+59798639 Msa0510660:CDS
GGAATATGAACGTGTACCTG+AGG 0.717370 3_4:+59798565 Msa0510660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCTTCGAACTTCTGAAATGT+GGG - chr3_4:59798405-59798424 None:intergenic 40.0%
CCACATTTCAGAAGTTCGAA+GGG + chr3_4:59798403-59798422 Msa0510660:CDS 40.0%
ATAGACAACCCTCTTGTTGA+AGG - chr3_4:59798468-59798487 None:intergenic 40.0%
TAGATCACTTGACCACCATA+GGG - chr3_4:59798596-59798615 None:intergenic 40.0%
GTCACTCATGGAATACATTC+AGG + chr3_4:59798675-59798694 Msa0510660:CDS 40.0%
AATGTGGGTGTGGTAGTCAT+TGG - chr3_4:59798390-59798409 None:intergenic 45.0%
!! GAACTTCTGAAATGTGGGTG+TGG - chr3_4:59798400-59798419 None:intergenic 45.0%
CCCACATTTCAGAAGTTCGA+AGG + chr3_4:59798402-59798421 Msa0510660:CDS 45.0%
!! CCCTTCGAACTTCTGAAATG+TGG - chr3_4:59798406-59798425 None:intergenic 45.0%
GAATACTCTCGCTACCCAAA+TGG + chr3_4:59798434-59798453 Msa0510660:CDS 45.0%
TGAAGGCCATCTCACCATTT+GGG - chr3_4:59798451-59798470 None:intergenic 45.0%
TTGAAGGCCATCTCACCATT+TGG - chr3_4:59798452-59798471 None:intergenic 45.0%
TGAGATGGCCTTCAACAAGA+GGG + chr3_4:59798457-59798476 Msa0510660:CDS 45.0%
CAACAAGAGGGTTGTCTATG+AGG + chr3_4:59798469-59798488 Msa0510660:CDS 45.0%
TATGAGGAGGATGCGATTGA+AGG + chr3_4:59798485-59798504 Msa0510660:CDS 45.0%
TCTCAACGATTTCAGGGTTG+TGG - chr3_4:59798532-59798551 None:intergenic 45.0%
ACAACCCTGAAATCGTTGAG+AGG + chr3_4:59798531-59798550 Msa0510660:CDS 45.0%
CAACCCTGAAATCGTTGAGA+GGG + chr3_4:59798532-59798551 Msa0510660:CDS 45.0%
CAACCCTCTCAACGATTTCA+GGG - chr3_4:59798538-59798557 None:intergenic 45.0%
TCAACCCTCTCAACGATTTC+AGG - chr3_4:59798539-59798558 None:intergenic 45.0%
AATCGTTGAGAGGGTTGAAG+TGG + chr3_4:59798541-59798560 Msa0510660:CDS 45.0%
GGAATATGAACGTGTACCTG+AGG + chr3_4:59798565-59798584 Msa0510660:CDS 45.0%
ATGAACGTGTACCTGAGGTT+AGG + chr3_4:59798570-59798589 Msa0510660:CDS 45.0%
GTAGATCACTTGACCACCAT+AGG - chr3_4:59798597-59798616 None:intergenic 45.0%
TGGTCAAGTGATCTACGAAG+AGG + chr3_4:59798601-59798620 Msa0510660:CDS 45.0%
CCTTCGTGGGTAATGTTGAT+TGG - chr3_4:59798642-59798661 None:intergenic 45.0%
CCAATCAACATTACCCACGA+AGG + chr3_4:59798639-59798658 Msa0510660:CDS 45.0%
AACATTACCCACGAAGGAAC+AGG + chr3_4:59798645-59798664 Msa0510660:CDS 45.0%
CATTTGACCTGTTCCTTCGT+GGG - chr3_4:59798655-59798674 None:intergenic 45.0%
ACAGGTCAAATGGTCACTCA+TGG + chr3_4:59798663-59798682 Msa0510660:CDS 45.0%
TCGCTACCCAAATGGTGAGA+TGG + chr3_4:59798442-59798461 Msa0510660:CDS 50.0%
GTGAGATGGCCTTCAACAAG+AGG + chr3_4:59798456-59798475 Msa0510660:CDS 50.0%
CAAGAGGGTTGTCTATGAGG+AGG + chr3_4:59798472-59798491 Msa0510660:CDS 50.0%
CAACGATTTCAGGGTTGTGG+TGG - chr3_4:59798529-59798548 None:intergenic 50.0%
AGTGATCTACGAAGAGGACG+TGG + chr3_4:59798607-59798626 Msa0510660:CDS 50.0%
CCATTTGACCTGTTCCTTCG+TGG - chr3_4:59798656-59798675 None:intergenic 50.0%
CCACGAAGGAACAGGTCAAA+TGG + chr3_4:59798653-59798672 Msa0510660:CDS 50.0%
!! GGGTAGCGAGAGTATTCGCT+AGG - chr3_4:59798431-59798450 None:intergenic 55.0%
GCGATTGAAGGATCACGCCA+TGG + chr3_4:59798497-59798516 Msa0510660:CDS 55.0%
!! TTTCAGGGTTGTGGTGGTGG+CGG - chr3_4:59798523-59798542 None:intergenic 55.0%
CGATTTCAGGGTTGTGGTGG+TGG - chr3_4:59798526-59798545 None:intergenic 55.0%
CGTTGAGAGGGTTGAAGTGG+TGG + chr3_4:59798544-59798563 Msa0510660:CDS 55.0%
GTACCTGAGGTTAGGCCCTA+TGG + chr3_4:59798578-59798597 Msa0510660:CDS 55.0%
CCACCATAGGGCCTAACCTC+AGG - chr3_4:59798584-59798603 None:intergenic 60.0%
CCTGAGGTTAGGCCCTATGG+TGG + chr3_4:59798581-59798600 Msa0510660:CDS 60.0%
!! GGTTGTGGTGGTGGCGGCCA+TGG - chr3_4:59798517-59798536 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 59798377 59798703 59798377 ID=Msa0510660;Name=Msa0510660
chr3_4 mRNA 59798377 59798703 59798377 ID=Msa0510660-mRNA-1;Parent=Msa0510660;Name=Msa0510660-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|108
chr3_4 exon 59798377 59798703 59798377 ID=Msa0510660-mRNA-1:exon:14205;Parent=Msa0510660-mRNA-1
chr3_4 CDS 59798377 59798703 59798377 ID=Msa0510660-mRNA-1:cds;Parent=Msa0510660-mRNA-1
Gene Sequence

>Msa0510660

ATGATGAGATCCAATGACTACCACACCCACATTTCAGAAGTTCGAAGGGAGCCTAGCGAATACTCTCGCTACCCAAATGGTGAGATGGCCTTCAACAAGAGGGTTGTCTATGAGGAGGATGCGATTGAAGGATCACGCCATGGCCGCCACCACCACAACCCTGAAATCGTTGAGAGGGTTGAAGTGGTGGAATATGAACGTGTACCTGAGGTTAGGCCCTATGGTGGTCAAGTGATCTACGAAGAGGACGTGGATGTTGAGACCAATCAACATTACCCACGAAGGAACAGGTCAAATGGTCACTCATGGAATACATTCAGGCCATAA

Protein sequence

>Msa0510660

MMRSNDYHTHISEVRREPSEYSRYPNGEMAFNKRVVYEEDAIEGSRHGRHHHNPEIVERVEVVEYERVPEVRPYGGQVIYEEDVDVETNQHYPRRNRSNGHSWNTFRP*