AlfalfaGEDB | Msa1454330

M. sativa cultivar zhongmu-4 / Msa1454330

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791
PFAM: PF02458.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454330	sp\|A0A498KFL4\|ATGSA_MALDO	66.667	81	27	0	287	367	139	219	2.71e-28	118

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454330	A0A396I0R2	65.411	292	73	5	17	296	17	292	1.39e-84	275

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa1454330	0.834402	3.059604e-56	-8.615850e-47
Msa0003450	Msa1454330	0.845418	4.110897e-59	-8.615850e-47
Msa0005500	Msa1454330	0.856090	4.069777e-62	-8.615850e-47
Msa0007810	Msa1454330	0.806100	9.692237e-50	-8.615850e-47
Msa0011060	Msa1454330	0.816091	6.611784e-52	-8.615850e-47
Msa0014970	Msa1454330	0.856952	2.270761e-62	-8.615850e-47
Msa0016830	Msa1454330	0.804633	1.966718e-49	-8.615850e-47
Msa0025060	Msa1454330	0.861922	7.296665e-64	-8.615850e-47
Msa0025090	Msa1454330	0.856027	4.244980e-62	-8.615850e-47
Msa0026120	Msa1454330	0.830618	2.653316e-55	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454330	MtrunA17_Chr4g0010341	65.411	292	73	5	17	296	17	292	2.68e-88	275
Msa1454330	MtrunA17_Chr7g0273781	89.691	97	10	0	287	383	172	268	3.63e-55	189
Msa1454330	MtrunA17_Chr7g0273791	95.455	88	4	0	296	383	148	235	2.08e-54	186
Msa1454330	MtrunA17_Chr5g0433641	80.000	100	19	1	285	383	137	236	5.99e-46	163
Msa1454330	MtrunA17_Chr7g0273731	80.682	88	17	0	296	383	231	318	5.22e-44	159
Msa1454330	MtrunA17_Chr7g0273721	73.000	100	26	1	285	383	138	237	6.97e-42	152
Msa1454330	MtrunA17_Chr7g0273761	72.449	98	26	1	287	383	512	609	3.19e-41	154
Msa1454330	MtrunA17_Chr7g0273761	74.157	89	22	1	296	383	33	121	4.06e-38	145
Msa1454330	MtrunA17_Chr7g0273691	85.915	71	9	1	314	383	1	71	5.73e-36	127
Msa1454330	MtrunA17_Chr0c01g0489551	73.267	101	23	1	1	97	1	101	1.59e-34	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1454330	AT5G17540.1	45.122	82	45	0	285	366	137	218	3.46e-17	83.2
Msa1454330	AT3G03480.1	46.250	80	42	1	287	365	149	228	2.71e-16	80.5
Msa1454330	AT5G41040.1	42.254	71	40	1	296	366	168	237	2.18e-11	65.5
Msa1454330	AT5G41040.2	42.254	71	40	1	296	366	152	221	2.34e-11	65.5
Msa1454330	AT3G48720.1	35.955	89	55	2	296	383	145	232	4.16e-11	64.7

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Find 127 sgRNAs with CRISPR-Local

Find 503 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTATGATAGCAGTTA+TGG	0.312366	tig0035128:-22995	None:intergenic
AAATGGTTGCATGTAATGCG+TGG	0.690356	tig0035128:-27929	None:intergenic
AAATTTGTTCGTATTCAAAA+TGG	0.285497	tig0035128:-27946	None:intergenic
AAGAGAATGAGAAAGAGCGC+TGG	0.619019	tig0035128:+23039	Msa1454330:CDS
AAGAGCCCGTATTCGGTTTG+AGG	0.426503	tig0035128:+23256	Msa1454330:CDS
AAGGGAATGCTTTGTGGTGG+AGG	0.402471	tig0035128:-22622	None:intergenic
AATACTTACGAGTTTCCACT+AGG	0.595610	tig0035128:-22844	None:intergenic
AATATCCTGGATGTACAGGA+GGG	0.685304	tig0035128:-22465	None:intergenic
AATCGACCATGAGAATCTCT+CGG	0.606484	tig0035128:-22811	None:intergenic
AATGTGGTCGGAGAGGATGG+TGG	0.648444	tig0035128:+22754	Msa1454330:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAAAAAGGAAAGAGCAA+GGG	+	tig0035128:22271-22290	Msa1454330:CDS	30.0%
ATATTATAGTTGGTCCCAAA+AGG	-	tig0035128:22505-22524	None:intergenic	30.0%
ACAACTACAACAAGAAAATG+TGG	+	tig0035128:22738-22757	Msa1454330:CDS	30.0%
TTAACCAAGTCATCAAAGAT+AGG	-	tig0035128:23314-23333	None:intergenic	30.0%
TGTCTATAAAATGTCATGAC+TGG	+	tig0035128:23917-23936	Msa1454330:intron	30.0%
ACTGGAAAATTATAACTGCT+GGG	+	tig0035128:23935-23954	Msa1454330:intron	30.0%
AGCAATAAGCTGCATATATA+GGG	-	tig0035128:24168-24187	None:intergenic	30.0%
GCTTATTGCTCAATAGAAAA+CGG	+	tig0035128:24178-24197	Msa1454330:intron	30.0%
TAAACACAAGTATGACAAGT+TGG	+	tig0035128:24208-24227	Msa1454330:intron	30.0%
TAGATTATAGCAGAGAAACA+TGG	-	tig0035128:24337-24356	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0035128	gene	22262	28001	22262	ID=Msa1454330;Name=Msa1454330
tig0035128	mRNA	22262	28001	22262	ID=Msa1454330-mRNA-1;Parent=Msa1454330;Name=Msa1454330-mRNA-1;_AED=0.17;_eAED=0.18;_QI=0\|0.66\|0.25\|1\|1\|1\|4\|0\|383
tig0035128	exon	22262	22857	22262	ID=Msa1454330-mRNA-1:exon:19654;Parent=Msa1454330-mRNA-1
tig0035128	exon	22950	23076	22950	ID=Msa1454330-mRNA-1:exon:19655;Parent=Msa1454330-mRNA-1
tig0035128	exon	23167	23328	23167	ID=Msa1454330-mRNA-1:exon:19656;Parent=Msa1454330-mRNA-1
tig0035128	exon	27738	28001	27738	ID=Msa1454330-mRNA-1:exon:19657;Parent=Msa1454330-mRNA-1
tig0035128	CDS	22262	22857	22262	ID=Msa1454330-mRNA-1:cds;Parent=Msa1454330-mRNA-1
tig0035128	CDS	22950	23076	22950	ID=Msa1454330-mRNA-1:cds;Parent=Msa1454330-mRNA-1
tig0035128	CDS	23167	23328	23167	ID=Msa1454330-mRNA-1:cds;Parent=Msa1454330-mRNA-1
tig0035128	CDS	27738	28001	27738	ID=Msa1454330-mRNA-1:cds;Parent=Msa1454330-mRNA-1

Gene Sequence

>Msa1454330

ATGCCTCCGAAACAAAAAGGAAAGAGCAAGGGCAACAATAGGAAGCGGTACACTACTCATGTCGTGGACCCGGCAACAGAAGCGGGATTAAACCCCATACCTGCCATTTCAAATGCATCTTCTAGCCAGGGATCATCTGCGTCCTCCCAAGGTAGTGTAGCTGCCCCTGCTTTGAGTTCTGGTCCTCCTCCCACGTACACGGGCCCTCCTGTACATCCAGGATATTATGGCACCATACCTCCTTTTGGGACCAACTATAATATCCCACCATATTCAGTCCCACCACAAAACATTAACTTTCCCCAACAACAACTAATCCCTCCACTACAAAACATTCCCTTTCCCCAACAACAACAAACCCCTCCACCACAAAGCATTCCCTTTTCCCAACAACAACAAATTCCTCTACAACAAAACTCACCCTTTCCCCAACAACAACAAAACCCACCACAACCCCAACAACAACAAAACCCACAACAACTACAACAAGAAAATGTGGTCGGAGAGGATGGTGGTGGAGAGGTGATTGATGATGTTTTCCCGGATTATCCGAGAGATTCTCATGGTCGATTCATGTTAAAACCTAGTGGAAACTCCTTTTTACCTTGCAAACCGGCTGCTGAAGCTATCAGAGCCATCATCCATAACTGCTATCATAAATTTTGGAAGACATATGGTGATGTGGAAGAGAATGAGAAAGAGCGCTGGTTTAGGCTCTTTGAGGGTAAGTGTTCTTGGAAAGCCGAGTATCATGATATAATGCGGCGTAATTTTCATATTAGAGCTTCTGCGCGTTTTTCGGATTTGCTTAGAAGAGCCCGTATTCGGTTTGAGGAGACAAGAAAGCGACCACATTGGATTGGCCCGCCTATCTTTGATGACTTGGTCACACGTCTCAAGTGTGGTGGGTTCATAGTGGCCATGAATTGGAACCATACAATGGGTGATGCAGCTGGTTTGAGACAATTCATGACTGCATGGGCAGAAATGGCTCGAGGAGCACATCGTCCTTCCATTCAACCTGTGTGGAATAGGGAGATTCTAATGGCAAGAGACCCTCCACGCATTACATGCAACCATTTTGAATACGAACAAATTTTTTCGCCAAATACCATTAAGGAAGAAGATACTGCTTCTCTAGTCCATCAA

Protein sequence

>Msa1454330

MPPKQKGKSKGNNRKRYTTHVVDPATEAGLNPIPAISNASSSQGSSASSQGSVAAPALSSGPPPTYTGPPVHPGYYGTIPPFGTNYNIPPYSVPPQNINFPQQQLIPPLQNIPFPQQQQTPPPQSIPFSQQQQIPLQQNSPFPQQQQNPPQPQQQQNPQQLQQENVVGEDGGGEVIDDVFPDYPRDSHGRFMLKPSGNSFLPCKPAAEAIRAIIHNCYHKFWKTYGDVEENEKERWFRLFEGKCSWKAEYHDIMRRNFHIRASARFSDLLRRARIRFEETRKRPHWIGPPIFDDLVTRLKCGGFIVAMNWNHTMGDAAGLRQFMTAWAEMARGAHRPSIQPVWNREILMARDPPRITCNHFEYEQIFSPNTIKEEDTASLVHQ