AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08512


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATTATGCATACCTTTC+TGG 0.079710 8.1:-76220212 None:intergenic
CCTGGAGTTCTTGAGTTAAT+AGG 0.246781 8.1:+76219277 MS.gene08512:CDS
GATAGACTTCCTTTGATTCC+TGG 0.345188 8.1:+76219259 MS.gene08512:CDS
GTTCTTGAGTTAATAGGAAT+TGG 0.346748 8.1:+76219283 MS.gene08512:CDS
AAAGATTGCTCGTGATGTTA+TGG 0.420998 8.1:+76218861 MS.gene08512:CDS
TAGCTAGTGTATTTGCACTA+TGG 0.434144 8.1:+76219107 MS.gene08512:CDS
GATGTTATGGTAATGGCAAC+AGG 0.459632 8.1:+76218874 MS.gene08512:CDS
TTTGCACTATGGAGCTCAGC+TGG 0.465802 8.1:+76219118 MS.gene08512:CDS
TGAAGATTCTTAACAAACTC+AGG 0.486308 8.1:-76218931 None:intergenic
TCAGTTGAGTCAACTTCTGT+TGG 0.503744 8.1:-76218904 None:intergenic
TAAATATGCAGTGGGTTCAT+TGG 0.505381 8.1:+76219081 MS.gene08512:CDS
CCTATTAACTCAAGAACTCC+AGG 0.507872 8.1:-76219277 None:intergenic
TTAATAGGAATTGGTTACAC+TGG 0.524822 8.1:+76219292 MS.gene08512:CDS
TAATAGGAATTGGTTACACT+GGG 0.540472 8.1:+76219293 MS.gene08512:CDS
TCAAGAACTCCAGGAATCAA+AGG 0.544788 8.1:-76219268 None:intergenic
TGCTCGTGATGTTATGGTAA+TGG 0.557462 8.1:+76218867 MS.gene08512:CDS
TTTGATGATAAATATGCAGT+GGG 0.562064 8.1:+76219073 MS.gene08512:CDS
ATTTGATGATAAATATGCAG+TGG 0.570116 8.1:+76219072 MS.gene08512:CDS
AATAGGAATTGGTTACACTG+GGG 0.760636 8.1:+76219294 MS.gene08512:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAACATAATTCT+AGG - chr8.1:76219031-76219050 None:intergenic 10.0%
!! ATAAAAATGTATTACAATAT+CGG - chr8.1:76219761-76219780 None:intergenic 10.0%
!! TATATGTATATATATATATG+AGG + chr8.1:76220093-76220112 MS.gene08512:intron 10.0%
!! TATTTCAAAAAAAAAATACA+AGG - chr8.1:76219392-76219411 None:intergenic 10.0%
!!! AATTATGTTATTTATTTTGT+TGG + chr8.1:76219033-76219052 MS.gene08512:intron 10.0%
!! TCAATAATAAACATTAAGAA+GGG - chr8.1:76219008-76219027 None:intergenic 15.0%
!!! TATTTTGTCATAAAATCAAA+TGG - chr8.1:76219938-76219957 None:intergenic 15.0%
!! AAATATAGAAGATGAATACT+AGG - chr8.1:76220152-76220171 None:intergenic 20.0%
!! CTTAGATTTAAATATATGAC+TGG + chr8.1:76219334-76219353 MS.gene08512:intron 20.0%
!! GTCAATAATAAACATTAAGA+AGG - chr8.1:76219009-76219028 None:intergenic 20.0%
!! TCTAATCTTATCAATTTGTA+AGG - chr8.1:76219801-76219820 None:intergenic 20.0%
!! TTACAAATTGATAAGATTAG+AGG + chr8.1:76219800-76219819 MS.gene08512:intron 20.0%
!!! TTTTTTTCCAGTAATTTCTA+GGG + chr8.1:76219878-76219897 MS.gene08512:intron 20.0%
! TAGGTTGTTAGAAATTGTAA+TGG + chr8.1:76218971-76218990 MS.gene08512:intron 25.0%
!! ATTTGATGATAAATATGCAG+TGG + chr8.1:76219072-76219091 MS.gene08512:CDS 25.0%
!! TATGAGGTATTATTCATCTT+TGG + chr8.1:76220109-76220128 MS.gene08512:intron 25.0%
!! TTTGATGATAAATATGCAGT+GGG + chr8.1:76219073-76219092 MS.gene08512:CDS 25.0%
!!! AATATCGCGTATTTGATTTT+TGG + chr8.1:76219365-76219384 MS.gene08512:intron 25.0%
!!! GTTTTTTTCCAGTAATTTCT+AGG + chr8.1:76219877-76219896 MS.gene08512:intron 25.0%
!!! TTGTTTTTTAAAATGATCCC+CGG + chr8.1:76219429-76219448 MS.gene08512:intron 25.0%
AAGAAAACCCTAGAAATTAC+TGG - chr8.1:76219888-76219907 None:intergenic 30.0%
AAGCACAATAGTATTTGACA+TGG - chr8.1:76219673-76219692 None:intergenic 30.0%
AATATCGGACTCTATCTATA+AGG - chr8.1:76219746-76219765 None:intergenic 30.0%
AGCACAATAGTATTTGACAT+GGG - chr8.1:76219672-76219691 None:intergenic 30.0%
ATCAAATGGCTTTCTTAAGA+TGG - chr8.1:76219924-76219943 None:intergenic 30.0%
ATGTCAAATACTATTGTGCT+TGG + chr8.1:76219672-76219691 MS.gene08512:intron 30.0%
CAATTACTTTCACTTGTAAC+AGG + chr8.1:76219231-76219250 MS.gene08512:intron 30.0%
GCATCAAGTGAAATAATGTT+GGG - chr8.1:76219564-76219583 None:intergenic 30.0%
GCTTTCTTAAGATGGTATTT+AGG - chr8.1:76219916-76219935 None:intergenic 30.0%
GTTCTTGAGTTAATAGGAAT+TGG + chr8.1:76219283-76219302 MS.gene08512:CDS 30.0%
TAATAGGAATTGGTTACACT+GGG + chr8.1:76219293-76219312 MS.gene08512:CDS 30.0%
TGAAGATTCTTAACAAACTC+AGG - chr8.1:76218934-76218953 None:intergenic 30.0%
TGCATCAAGTGAAATAATGT+TGG - chr8.1:76219565-76219584 None:intergenic 30.0%
TTAATAGGAATTGGTTACAC+TGG + chr8.1:76219292-76219311 MS.gene08512:CDS 30.0%
! ACTTGATGCATTGATATACA+TGG + chr8.1:76219575-76219594 MS.gene08512:intron 30.0%
! TATTTTGTTGGCATCATAGT+GGG + chr8.1:76219045-76219064 MS.gene08512:intron 30.0%
!! TTATTTTGTTGGCATCATAG+TGG + chr8.1:76219044-76219063 MS.gene08512:intron 30.0%
!!! TGTTTTTTAAAATGATCCCC+GGG + chr8.1:76219430-76219449 MS.gene08512:intron 30.0%
AAAGATTGCTCGTGATGTTA+TGG + chr8.1:76218861-76218880 MS.gene08512:CDS 35.0%
AATAGGAATTGGTTACACTG+GGG + chr8.1:76219294-76219313 MS.gene08512:CDS 35.0%
ACAATAGTATTTGACATGGG+GGG - chr8.1:76219669-76219688 None:intergenic 35.0%
ATCAAATACGCGATATTTGC+AGG - chr8.1:76219362-76219381 None:intergenic 35.0%
ATTGACATATAGATAGGGGA+TGG + chr8.1:76219698-76219717 MS.gene08512:intron 35.0%
CAAGAAGCTGTATGATACAT+AGG + chr8.1:76218952-76218971 MS.gene08512:intron 35.0%
CACAATAGTATTTGACATGG+GGG - chr8.1:76219670-76219689 None:intergenic 35.0%
CCTGCATTGAATAGTGTATA+AGG - chr8.1:76219613-76219632 None:intergenic 35.0%
CCTTATACACTATTCAATGC+AGG + chr8.1:76219610-76219629 MS.gene08512:intron 35.0%
GCACAATAGTATTTGACATG+GGG - chr8.1:76219671-76219690 None:intergenic 35.0%
TAGCTAGTGTATTTGCACTA+TGG + chr8.1:76219107-76219126 MS.gene08512:CDS 35.0%
TCAAATACGCGATATTTGCA+GGG - chr8.1:76219361-76219380 None:intergenic 35.0%
TTCTATATTTCACCTTGCAG+TGG + chr8.1:76220162-76220181 MS.gene08512:intron 35.0%
! GCATTGATATACATGGTTGA+TGG + chr8.1:76219582-76219601 MS.gene08512:intron 35.0%
! GGTGCATTGACATATAGATA+GGG + chr8.1:76219693-76219712 MS.gene08512:intron 35.0%
! GTGCATTGACATATAGATAG+GGG + chr8.1:76219694-76219713 MS.gene08512:intron 35.0%
! TAAATATGCAGTGGGTTCAT+TGG + chr8.1:76219081-76219100 MS.gene08512:CDS 35.0%
! TGGTGCATTGACATATAGAT+AGG + chr8.1:76219692-76219711 MS.gene08512:intron 35.0%
!! GATGGCCTTATAGAAGATAT+GGG + chr8.1:76219716-76219735 MS.gene08512:intron 35.0%
AATAGTATTTGACATGGGGG+GGG - chr8.1:76219667-76219686 None:intergenic 40.0%
ACACTATTCAATGCAGGACT+TGG + chr8.1:76219616-76219635 MS.gene08512:intron 40.0%
CAATAGTATTTGACATGGGG+GGG - chr8.1:76219668-76219687 None:intergenic 40.0%
CCTATTAACTCAAGAACTCC+AGG - chr8.1:76219280-76219299 None:intergenic 40.0%
CCTGGAGTTCTTGAGTTAAT+AGG + chr8.1:76219277-76219296 MS.gene08512:CDS 40.0%
GATAGACTTCCTTTGATTCC+TGG + chr8.1:76219259-76219278 MS.gene08512:CDS 40.0%
GCCATCCCATATCTTCTATA+AGG - chr8.1:76219724-76219743 None:intergenic 40.0%
GCCTTATAGAAGATATGGGA+TGG + chr8.1:76219720-76219739 MS.gene08512:intron 40.0%
GTCCATTTAAGCTGATAGGT+GGG + chr8.1:76219452-76219471 MS.gene08512:intron 40.0%
TCAAGAACTCCAGGAATCAA+AGG - chr8.1:76219271-76219290 None:intergenic 40.0%
TCAGTTGAGTCAACTTCTGT+TGG - chr8.1:76218907-76218926 None:intergenic 40.0%
TGCTCGTGATGTTATGGTAA+TGG + chr8.1:76218867-76218886 MS.gene08512:CDS 40.0%
!! GATATACATGGTTGATGGTC+AGG + chr8.1:76219587-76219606 MS.gene08512:intron 40.0%
!! GATGTTATGGTAATGGCAAC+AGG + chr8.1:76218874-76218893 MS.gene08512:CDS 40.0%
!! GGATGGCCTTATAGAAGATA+TGG + chr8.1:76219715-76219734 MS.gene08512:intron 40.0%
ATAGTATTTGACATGGGGGG+GGG - chr8.1:76219666-76219685 None:intergenic 45.0%
CCTATCAGCTTAAATGGACC+CGG - chr8.1:76219451-76219470 None:intergenic 45.0%
CTATCAGCTTAAATGGACCC+GGG - chr8.1:76219450-76219469 None:intergenic 45.0%
CTCCCACCTATCAGCTTAAA+TGG - chr8.1:76219457-76219476 None:intergenic 45.0%
CTTGTATGCAAACCACTGCA+AGG - chr8.1:76220177-76220196 None:intergenic 45.0%
GGTCCATTTAAGCTGATAGG+TGG + chr8.1:76219451-76219470 MS.gene08512:intron 45.0%
TATCAGCTTAAATGGACCCG+GGG - chr8.1:76219449-76219468 None:intergenic 45.0%
!!! GAGCTCAGCTGGTTTTATCT+CGG + chr8.1:76219129-76219148 MS.gene08512:CDS 45.0%
CCGGGTCCATTTAAGCTGAT+AGG + chr8.1:76219448-76219467 MS.gene08512:intron 50.0%
TAGTATTTGACATGGGGGGG+GGG - chr8.1:76219665-76219684 None:intergenic 50.0%
TTTGCACTATGGAGCTCAGC+TGG + chr8.1:76219118-76219137 MS.gene08512:CDS 50.0%
AGTATTTGACATGGGGGGGG+GGG - chr8.1:76219664-76219683 None:intergenic 55.0%
GTATTTGACATGGGGGGGGG+GGG - chr8.1:76219663-76219682 None:intergenic 60.0%
TATTTGACATGGGGGGGGGG+GGG - chr8.1:76219662-76219681 None:intergenic 60.0%
ATTTGACATGGGGGGGGGGG+GGG - chr8.1:76219661-76219680 None:intergenic 65.0%
TTTGACATGGGGGGGGGGGG+GGG - chr8.1:76219660-76219679 None:intergenic 70.0%
TTGACATGGGGGGGGGGGGG+GGG - chr8.1:76219659-76219678 None:intergenic 75.0%
! TGACATGGGGGGGGGGGGGG+GGG - chr8.1:76219658-76219677 None:intergenic 80.0%
! ACATGGGGGGGGGGGGGGGG+GGG - chr8.1:76219656-76219675 None:intergenic 85.0%
! GACATGGGGGGGGGGGGGGG+GGG - chr8.1:76219657-76219676 None:intergenic 85.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 76218857 76220219 76218857 ID=MS.gene08512
chr8.1 mRNA 76218857 76220219 76218857 ID=MS.gene08512.t1;Parent=MS.gene08512
chr8.1 exon 76218857 76218960 76218857 ID=MS.gene08512.t1.exon1;Parent=MS.gene08512.t1
chr8.1 CDS 76218857 76218960 76218857 ID=cds.MS.gene08512.t1;Parent=MS.gene08512.t1
chr8.1 exon 76219064 76219150 76219064 ID=MS.gene08512.t1.exon2;Parent=MS.gene08512.t1
chr8.1 CDS 76219064 76219150 76219064 ID=cds.MS.gene08512.t1;Parent=MS.gene08512.t1
chr8.1 exon 76219253 76219315 76219253 ID=MS.gene08512.t1.exon3;Parent=MS.gene08512.t1
chr8.1 CDS 76219253 76219315 76219253 ID=cds.MS.gene08512.t1;Parent=MS.gene08512.t1
chr8.1 exon 76220182 76220219 76220182 ID=MS.gene08512.t1.exon4;Parent=MS.gene08512.t1
chr8.1 CDS 76220182 76220219 76220182 ID=cds.MS.gene08512.t1;Parent=MS.gene08512.t1
Gene Sequence

>MS.gene08512

ATCGAAAGATTGCTCGTGATGTTATGGTAATGGCAACAGGAGATGCACCAACAGAAGTTGACTCAACTGAGCTACCTGAGTTTGTTAAGAATCTTCAAGAAGCTGTATGATACATAGGTTGTTAGAAATTGTAATGGAGTTTCTTTTTCCCTTCTTAATGTTTATTATTGACCTAGAATTATGTTATTTATTTTGTTGGCATCATAGTGGGACAAATTTGATGATAAATATGCAGTGGGTTCATTGGTTGTAGCTAGTGTATTTGCACTATGGAGCTCAGCTGGTTTTATCTCGGTATTAAGTTATTTAAATGCTAATTTTATTTGCATTATTTGATTATCAATTTATTAGATACAGATGATAAATGTCATAAGCAATTACTTTCACTTGTAACAGGCAATTGATAGACTTCCTTTGATTCCTGGAGTTCTTGAGTTAATAGGAATTGGTTACACTGGGGTAAGTCAAATAAATACACTTAGATTTAAATATATGACTGGTCCCTGCAAATATCGCGTATTTGATTTTTGGTCCTTGTATTTTTTTTTTGAAATACATCTTTGCAAGAAAAATTGTTTTTTAAAATGATCCCCGGGTCCATTTAAGCTGATAGGTGGGAGTCTGATGCTTACGTTACACATGCTAAAAAAAATAAAATCTGAAACTTAATTTAAAAAAAAAATATAAATAGATTTTTTCATATGCCCAACATTATTTCACTTGATGCATTGATATACATGGTTGATGGTCAGGCCTTATACACTATTCAATGCAGGACTTGGATTTTTGTTTTACACCCCCCCCCCCCCCCCCCCATGTCAAATACTATTGTGCTTGGTGCATTGACATATAGATAGGGGATGGCCTTATAGAAGATATGGGATGGCCTTATAGATAGAGTCCGATATTGTAATACATTTTTATATTTAGACATAACTCATCCTTACAAATTGATAAGATTAGAGGTGTCACTCTTTATAAACTCATCTCAAGCATTATATTTATTCAATGCGACACTTAGTTTTTTTCCAGTAATTTCTAGGGTTTTCTTTTAATCCTAAATACCATCTTAAGAAAGCCATTTGATTTTATGACAAAATACATATATACTAGCAAAAACAATATATATATATATATATATGTATATATATCTATATATATATATGTATATATATATCTATATATATGTATATATATATATATATATATATATATATATATGTGTATATATATATATATATGTATATATATATATGAGGTATTATTCATCTTTGGATTCAACTATAAGAAAACCTAGTATTCATCTTCTATATTTCACCTTGCAGTGGTTTGCATACAAGAACGTAGTTTTCAAGCCAGAAAG

Protein sequence

>MS.gene08512.t1

IERLLVMLWUWQQEMHQQKLTQLSYLSLLRIFKKLGTNLMINMQWVHWLULVYLHYGAQLVLSRQLIDFLUFLEFLSUUELVTLGGLHTRTUFSSQK