AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56592


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56592.t1 MTR_2g072530 92.053 302 24 0 1 302 150 451 0.0 574
MS.gene56592.t1 MTR_2g072550 52.239 268 56 6 35 302 31 226 1.85e-73 225
MS.gene56592.t1 MTR_7g094030 32.903 310 181 10 4 297 164 462 8.75e-37 137
MS.gene56592.t1 MTR_2g072720 31.511 311 184 11 1 297 182 477 3.02e-32 124
MS.gene56592.t1 MTR_2g082880 30.690 290 180 8 3 283 145 422 3.68e-30 118
MS.gene56592.t1 MTR_2g072730 30.952 294 190 8 1 287 158 445 6.74e-30 118
MS.gene56592.t1 MTR_1g025870 31.944 288 172 10 4 281 230 503 2.07e-29 117
MS.gene56592.t1 MTR_1g115250 31.535 241 152 6 47 282 201 433 3.79e-26 107
MS.gene56592.t1 MTR_2g082850 34.450 209 117 6 3 201 166 364 5.66e-26 106
MS.gene56592.t1 MTR_2g082800 32.129 249 158 7 2 249 162 400 2.48e-23 99.4
MS.gene56592.t1 MTR_4g032845 29.832 238 156 7 32 263 176 408 6.65e-23 98.2
MS.gene56592.t1 MTR_5g005700 29.352 293 193 7 11 297 208 492 3.69e-21 93.6
MS.gene56592.t1 MTR_6g022350 28.947 266 172 9 1 260 204 458 2.13e-19 88.6
MS.gene56592.t1 MTR_4g080590 26.923 286 197 8 6 285 163 442 2.00e-16 79.7
MS.gene56592.t1 MTR_2g072710 26.961 204 128 6 103 298 5 195 4.47e-16 75.5
MS.gene56592.t1 MTR_2g067210 31.905 210 125 8 4 204 202 402 6.43e-16 78.2
MS.gene56592.t1 MTR_7g094050 36.585 123 75 2 164 285 2 122 5.25e-14 68.2
MS.gene56592.t1 MTR_5g007090 28.214 280 162 10 10 285 212 456 2.18e-13 70.5
MS.gene56592.t1 MTR_4g080787 25.566 309 202 10 1 296 170 463 2.51e-13 70.5
MS.gene56592.t1 MTR_8g006910 28.000 250 154 9 11 251 199 431 1.90e-11 64.7
MS.gene56592.t1 MTR_7g023310 24.164 269 187 7 5 262 165 427 3.50e-11 63.9
MS.gene56592.t1 MTR_5g007110 36.364 88 54 2 176 263 2 87 7.23e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56592.t1 AT3G14710 24.590 305 197 7 2 296 160 441 1.06e-14 74.3

Find 36 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAGTATTTCAGTTTAA+AGG 0.139696 2.1:-26111692 MS.gene56592:CDS
AAACAAGAGTGTTAAGAATA+GGG 0.284563 2.1:+26112196 None:intergenic
GCGTCGACTATGAACCTTAA+TGG 0.307360 2.1:+26112642 None:intergenic
ATCATGTAAATATGCCATTA+AGG 0.324990 2.1:-26112656 MS.gene56592:CDS
TGTTGTTGATGAAGATAAAA+AGG 0.327183 2.1:-26112533 MS.gene56592:CDS
TTCACCAATCTGCCTGTCTT+TGG 0.335476 2.1:-26112285 MS.gene56592:CDS
AAAACAAGAGTGTTAAGAAT+AGG 0.349193 2.1:+26112195 None:intergenic
AAACTAATGTTCTGTGTTGT+TGG 0.384561 2.1:+26112736 None:intergenic
GAACGAGCACGATCTTTGTT+TGG 0.397360 2.1:-26111660 MS.gene56592:CDS
TACATGATTCATTTGAATGA+TGG 0.398273 2.1:+26112671 None:intergenic
GCTTCAAACACTTTGAGAAT+CGG 0.411946 2.1:+26112775 None:intergenic
CTTCAAACACTTTGAGAATC+GGG 0.419619 2.1:+26112776 None:intergenic
TGTTGATTTGAAGAAAGTTA+AGG 0.423111 2.1:-26111561 MS.gene56592:CDS
TGTTAAGAATAGGGGAATTG+TGG 0.424474 2.1:+26112205 None:intergenic
CCTTCCAAAGACAGGCAGAT+TGG 0.430732 2.1:+26112281 None:intergenic
CTCAAAGTGTTTGAAGCTAG+AGG 0.446938 2.1:-26112769 MS.gene56592:CDS
GTTGGCAGATGCAGCAATCA+TGG 0.478397 2.1:-26111627 MS.gene56592:CDS
ATCGAAAACTTCTCAAGTAA+AGG 0.483526 2.1:+26112706 None:intergenic
TAAGTTAACCTTCCAAAGAC+AGG 0.506614 2.1:+26112273 None:intergenic
TTGCAGTTGTTAGAACACTT+TGG 0.513678 2.1:+26111521 None:intergenic
AACTTATCTGCAACTAAATG+AGG 0.518407 2.1:-26112256 MS.gene56592:CDS
CCAATCTGCCTGTCTTTGGA+AGG 0.521176 2.1:-26112281 MS.gene56592:CDS
AACAAGAGTGTTAAGAATAG+GGG 0.527875 2.1:+26112197 None:intergenic
CTAACAGATTTCTCCTACGA+GGG 0.532743 2.1:-26112619 MS.gene56592:CDS
AACAGAAGCATTACGAATTG+AGG 0.535694 2.1:+26112561 None:intergenic
CCTCGTAGGAGAAATCTGTT+AGG 0.536945 2.1:+26112620 None:intergenic
GGAGATGATGATGATATCAC+CGG 0.566062 2.1:-26111606 MS.gene56592:CDS
CTGCAACTAAATGAGGTCAC+TGG 0.573768 2.1:-26112249 MS.gene56592:CDS
TCAAAGTGTTTGAAGCTAGA+GGG 0.585679 2.1:-26112768 MS.gene56592:CDS
TTGTTTGGTAAAATTCGTGT+TGG 0.601366 2.1:-26111645 MS.gene56592:CDS
AGATGTCTTTACTTCTGCAA+CGG 0.610724 2.1:-26111738 MS.gene56592:CDS
TTGAAGCTAGAGGGTGCGAA+TGG 0.623897 2.1:-26112759 MS.gene56592:CDS
TTGTTCAACATCACCCTCGT+AGG 0.671848 2.1:+26112606 None:intergenic
TCTCAAACAAATCGATAACG+TGG 0.686009 2.1:-26112473 MS.gene56592:CDS
CCTAACAGATTTCTCCTACG+AGG 0.694192 2.1:-26112620 MS.gene56592:CDS
AAGCTTGACAGAAAGCAGTG+AGG 0.745455 2.1:+26111713 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAATTTCTTTTAACAAA+AGG + chr2.1:26112245-26112264 None:intergenic 10.0%
!!! AGTGTTTATTTTTTTGAAGA+AGG + chr2.1:26112341-26112360 None:intergenic 20.0%
!!! GTTTTACTTAATGTAAGTTT+AGG - chr2.1:26112211-26112230 MS.gene56592:CDS 20.0%
!!! TTCAAAGTATTTCAGTTTAA+AGG - chr2.1:26112696-26112715 MS.gene56592:CDS 20.0%
! AACATAACTGCTTATGTATA+AGG + chr2.1:26112509-26112528 None:intergenic 25.0%
! ATCATGTAAATATGCCATTA+AGG - chr2.1:26111732-26111751 MS.gene56592:CDS 25.0%
! CATGACACAAAAAAATTTCA+GGG - chr2.1:26112609-26112628 MS.gene56592:CDS 25.0%
! GAGTTTATGAAAACAACTTA+AGG + chr2.1:26112409-26112428 None:intergenic 25.0%
! TACATGATTCATTTGAATGA+TGG + chr2.1:26111720-26111739 None:intergenic 25.0%
! TCATGACACAAAAAAATTTC+AGG - chr2.1:26112608-26112627 MS.gene56592:CDS 25.0%
! TGAAGATAAAAAGGATAGAA+TGG - chr2.1:26111864-26111883 MS.gene56592:intron 25.0%
! TTCAAAAACTTAGCATTTCT+GGG - chr2.1:26111536-26111555 MS.gene56592:CDS 25.0%
!! AGTTATATCTTTTGAACAAG+TGG + chr2.1:26111584-26111603 None:intergenic 25.0%
!! ATTAAGTTGTTTATCCAGAA+AGG - chr2.1:26112540-26112559 MS.gene56592:CDS 25.0%
!! TGTTGTTGATGAAGATAAAA+AGG - chr2.1:26111855-26111874 MS.gene56592:intron 25.0%
!! TTAAGTTGTTTATCCAGAAA+GGG - chr2.1:26112541-26112560 MS.gene56592:CDS 25.0%
!! TTTCTTTTACGATACATGAA+CGG - chr2.1:26111972-26111991 MS.gene56592:intron 25.0%
!!! AAAACAAGAGTGTTAAGAAT+AGG + chr2.1:26112196-26112215 None:intergenic 25.0%
!!! AAACAAGAGTGTTAAGAATA+GGG + chr2.1:26112195-26112214 None:intergenic 25.0%
!!! GCTAAAGTTATTGTTTTACA+AGG - chr2.1:26111942-26111961 MS.gene56592:intron 25.0%
!!! TGTTGATTTGAAGAAAGTTA+AGG - chr2.1:26112827-26112846 MS.gene56592:CDS 25.0%
AAACTAATGTTCTGTGTTGT+TGG + chr2.1:26111655-26111674 None:intergenic 30.0%
AACTTATCTGCAACTAAATG+AGG - chr2.1:26112132-26112151 MS.gene56592:intron 30.0%
AAGGATAGAATGGAAAAACT+TGG - chr2.1:26111874-26111893 MS.gene56592:intron 30.0%
ATCGAAAACTTCTCAAGTAA+AGG + chr2.1:26111685-26111704 None:intergenic 30.0%
CTTCAAAAACTTAGCATTTC+TGG - chr2.1:26111535-26111554 MS.gene56592:CDS 30.0%
TCAAAAACTTAGCATTTCTG+GGG - chr2.1:26111537-26111556 MS.gene56592:CDS 30.0%
TTGTTTGGTAAAATTCGTGT+TGG - chr2.1:26112743-26112762 MS.gene56592:CDS 30.0%
! AGAAGTTTTCGATAGCAATA+TGG - chr2.1:26111692-26111711 MS.gene56592:CDS 30.0%
! CAGAAATGCTAAGTTTTTGA+AGG + chr2.1:26111537-26111556 None:intergenic 30.0%
! TAAAATTTTCCTTGTCCTAC+AGG - chr2.1:26112057-26112076 MS.gene56592:intron 30.0%
!! AACAAGAGTGTTAAGAATAG+GGG + chr2.1:26112194-26112213 None:intergenic 30.0%
!! TGCTAAGTTTTTGAAGGTTT+TGG + chr2.1:26111531-26111550 None:intergenic 30.0%
AGATGTCTTTACTTCTGCAA+CGG - chr2.1:26112650-26112669 MS.gene56592:CDS 35.0%
GTGTTTAGCGTTGTTTAGAT+TGG - chr2.1:26112276-26112295 MS.gene56592:CDS 35.0%
TAAGTTAACCTTCCAAAGAC+AGG + chr2.1:26112118-26112137 None:intergenic 35.0%
TCTCAAACAAATCGATAACG+TGG - chr2.1:26111915-26111934 MS.gene56592:intron 35.0%
TGTTAAGAATAGGGGAATTG+TGG + chr2.1:26112186-26112205 None:intergenic 35.0%
TTGCAGTTGTTAGAACACTT+TGG + chr2.1:26112870-26112889 None:intergenic 35.0%
! CTTCAAACACTTTGAGAATC+GGG + chr2.1:26111615-26111634 None:intergenic 35.0%
! GCTTCAAACACTTTGAGAAT+CGG + chr2.1:26111616-26111635 None:intergenic 35.0%
! TCAAAGTGTTTGAAGCTAGA+GGG - chr2.1:26111620-26111639 MS.gene56592:CDS 35.0%
!! AACAGAAGCATTACGAATTG+AGG + chr2.1:26111830-26111849 None:intergenic 35.0%
!! GTTGTTTTCATAAACTCTCG+AGG - chr2.1:26112412-26112431 MS.gene56592:intron 35.0%
!! TTTTGGAGACAAACAGATAC+GGG + chr2.1:26111514-26111533 None:intergenic 35.0%
!!! GTTTTGGAGACAAACAGATA+CGG + chr2.1:26111515-26111534 None:intergenic 35.0%
CTAACAGATTTCTCCTACGA+GGG - chr2.1:26111769-26111788 MS.gene56592:intron 40.0%
TACCATGCCTTAAAACCTGT+AGG + chr2.1:26112075-26112094 None:intergenic 40.0%
! CTCAAAGTGTTTGAAGCTAG+AGG - chr2.1:26111619-26111638 MS.gene56592:CDS 40.0%
! GGAGATGATGATGATATCAC+CGG - chr2.1:26112782-26112801 MS.gene56592:CDS 40.0%
! TCCTTGTCCTACAGGTTTTA+AGG - chr2.1:26112065-26112084 MS.gene56592:intron 40.0%
!!! TGATGATATCACCGGCTTTT+TGG - chr2.1:26112790-26112809 MS.gene56592:CDS 40.0%
AAGCTTGACAGAAAGCAGTG+AGG + chr2.1:26112678-26112697 None:intergenic 45.0%
CCTAACAGATTTCTCCTACG+AGG - chr2.1:26111768-26111787 MS.gene56592:intron 45.0%
CCTCGTAGGAGAAATCTGTT+AGG + chr2.1:26111771-26111790 None:intergenic 45.0%
CTGCAACTAAATGAGGTCAC+TGG - chr2.1:26112139-26112158 MS.gene56592:intron 45.0%
GAACGAGCACGATCTTTGTT+TGG - chr2.1:26112728-26112747 MS.gene56592:CDS 45.0%
GCCTTAAAACCTGTAGGACA+AGG + chr2.1:26112069-26112088 None:intergenic 45.0%
TATGCAACGAAGACCCTTTC+TGG + chr2.1:26112557-26112576 None:intergenic 45.0%
TTCACCAATCTGCCTGTCTT+TGG - chr2.1:26112103-26112122 MS.gene56592:intron 45.0%
TTGTTCAACATCACCCTCGT+AGG + chr2.1:26111785-26111804 None:intergenic 45.0%
!! GCGTCGACTATGAACCTTAA+TGG + chr2.1:26111749-26111768 None:intergenic 45.0%
!!! GTCCTACAGGTTTTAAGGCA+TGG - chr2.1:26112070-26112089 MS.gene56592:intron 45.0%
CCAATCTGCCTGTCTTTGGA+AGG - chr2.1:26112107-26112126 MS.gene56592:intron 50.0%
CCTTCCAAAGACAGGCAGAT+TGG + chr2.1:26112110-26112129 None:intergenic 50.0%
GCATATCGCAGCCAAAAAGC+CGG + chr2.1:26112804-26112823 None:intergenic 50.0%
GTTGGCAGATGCAGCAATCA+TGG - chr2.1:26112761-26112780 MS.gene56592:CDS 50.0%
! TTGAAGCTAGAGGGTGCGAA+TGG - chr2.1:26111629-26111648 MS.gene56592:CDS 50.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 26111490 26112920 26111490 ID=MS.gene56592
chr2.1 mRNA 26111490 26112920 26111490 ID=MS.gene56592.t1;Parent=MS.gene56592
chr2.1 exon 26112447 26112920 26112447 ID=MS.gene56592.t1.exon1;Parent=MS.gene56592.t1
chr2.1 CDS 26112447 26112920 26112447 ID=cds.MS.gene56592.t1;Parent=MS.gene56592.t1
chr2.1 exon 26112188 26112331 26112188 ID=MS.gene56592.t1.exon2;Parent=MS.gene56592.t1
chr2.1 CDS 26112188 26112331 26112188 ID=cds.MS.gene56592.t1;Parent=MS.gene56592.t1
chr2.1 exon 26111490 26111780 26111490 ID=MS.gene56592.t1.exon3;Parent=MS.gene56592.t1
chr2.1 CDS 26111490 26111780 26111490 ID=cds.MS.gene56592.t1;Parent=MS.gene56592.t1
Gene Sequence

>MS.gene56592

ATGAGATGTACTCTTAGTCTTCCCGTATCTGTTTGTCTCCAAAACCTTCAAAAACTTAGCATTTCTGGGGTTAAATTAGTGAGTGATTCGTCCACTTGTTCAAAAGATATAACTCTCAATTTCCCGATTCTCAAAGTGTTTGAAGCTAGAGGGTGCGAATGGCCAACAACACAGAACATTAGTTTACAAGTACCTTTACTTGAGAAGTTTTCGATAGCAATATGGAACCATCATTCAAATGAATCATGTAAATATGCCATTAAGGTTCATAGTCGACGCCTAACAGATTTCTCCTACGAGGGTGATGTTGAACAAGACATCGTTCTATGTGATTCGTCCTCAATTCGTAATGCTTCTGTTGTGATTGTTGTTGATGAAGATAAAAAGGATAGAATGGAAAAACTTGGATTTCAAGCACGTAGTCTTCTCAAACAAATCGATAACGTGGAGCAGCTAAAGTTATTGTTTTACAAGGTATGTTTTTTCTTTTACGATACATGAACGGAAATAGTTTTGTATGACAAAAACATGTTGTGATAATTGAAAAAAAAAATCGCAGAATTTTAATAAAATTTTCCTTGTCCTACAGGTTTTAAGGCATGGTAAAGATATTTTCACCAATCTGCCTGTCTTTGGAAGGTTAACTTATCTGCAACTAAATGAGGTCACTGGTGAAGCTTTGTTGCATTTACTCCACAATTCCCCTATTCTTAACACTCTTGTTTTACTTAATGTAAGTTTAGGTACTCTAGCCTTTTGTTAAAAGAAATTTAATGTCTTGTTAATGTGTTTAGCGTTGTTTAGATTGGCATATTTGAGTTTATCTACTAGTATAAACACTTGTCAGACCTTCTTCAAAAAAATAAACACTTGTAAGAGTTTTTGAGAGAGCTTATAGAAACAACTTATGACATGTCCTTAAGTTGTTTTCATAAACTCTCGAGGATAGTTTATGAAAACAACTTATAGCTTATATGAAATCAGTTAGACTTAATTTATCTTTTATTATAAAAATTCCTTATACATAAGCAGTTATGTTATAAGCGCTTAATTAAGTTGTTTATCCAGAAAGGGTCTTCGTTGCATATATCATAAACTAATTTAAATGATAGAAAAACTCATGACACAAAAAAATTTCAGGGAGTAGCTGATTTAGACAAAGATGTCTTTACTTCTGCAACGGTGCCTCACTGCTTTCTGTCAAGCTTCAAAGTATTTCAGTTTAAAGGATTTAATGCGAACGAGCACGATCTTTGTTTGGTAAAATTCGTGTTGGCAGATGCAGCAATCATGGAGATGATGATGATATCACCGGCTTTTTGGCTGCGATATGCAAATGTTGATTTGAAGAAAGTTAAGGAGCAGATACTCTCATTACCAAAGTGTTCTAACAACTGCAAGATAGAATTTTCTGATATCAGTAGATCCTGA

Protein sequence

>MS.gene56592.t1

MRCTLSLPVSVCLQNLQKLSISGVKLVSDSSTCSKDITLNFPILKVFEARGCEWPTTQNISLQVPLLEKFSIAIWNHHSNESCKYAIKVHSRRLTDFSYEGDVEQDIVLCDSSSIRNASVVIVVDEDKKDRMEKLGFQARSLLKQIDNVEQLKLLFYKVLRHGKDIFTNLPVFGRLTYLQLNEVTGEALLHLLHNSPILNTLVLLNGVADLDKDVFTSATVPHCFLSSFKVFQFKGFNANEHDLCLVKFVLADAAIMEMMMISPAFWLRYANVDLKKVKEQILSLPKCSNNCKIEFSDISRS