Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1450130 | sp|Q9M2Z5|FBL53_ARATH | 68.562 | 299 | 92 | 2 | 9 | 305 | 11 | 309 | 1.37e-151 | 429 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1450130 | A0A396JYJ5 | 99.344 | 305 | 2 | 0 | 1 | 305 | 58 | 362 | 0.0 | 621 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015370 | Msa1450130 | -0.807859 | 4.113177e-50 | -8.615850e-47 |
| Msa0018870 | Msa1450130 | -0.842987 | 1.847844e-58 | -8.615850e-47 |
| Msa0027540 | Msa1450130 | -0.815784 | 7.740433e-52 | -8.615850e-47 |
| Msa0032040 | Msa1450130 | -0.806505 | 7.964061e-50 | -8.615850e-47 |
| Msa0036580 | Msa1450130 | 0.802146 | 6.444167e-49 | -8.615850e-47 |
| Msa0038350 | Msa1450130 | 0.815500 | 8.957170e-52 | -8.615850e-47 |
| Msa0039270 | Msa1450130 | 0.874047 | 9.163838e-68 | -8.615850e-47 |
| Msa0043240 | Msa1450130 | 0.820331 | 7.258119e-53 | -8.615850e-47 |
| Msa0043960 | Msa1450130 | 0.812004 | 5.271815e-51 | -8.615850e-47 |
| Msa0049990 | Msa1450130 | -0.801564 | 8.485204e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1450130 | MtrunA17_Chr1g0203471 | 99.344 | 305 | 2 | 0 | 1 | 305 | 58 | 362 | 0.0 | 621 |
| Msa1450130 | MtrunA17_Chr1g0203481 | 91.475 | 305 | 26 | 0 | 1 | 305 | 1 | 305 | 0.0 | 572 |
| Msa1450130 | MtrunA17_Chr4g0055171 | 48.136 | 295 | 140 | 2 | 12 | 305 | 21 | 303 | 1.72e-97 | 289 |
| Msa1450130 | MtrunA17_Chr1g0203501 | 59.649 | 171 | 65 | 1 | 139 | 305 | 2 | 172 | 4.06e-65 | 202 |
| Msa1450130 | MtrunA17_Chr4g0019981 | 55.952 | 168 | 72 | 2 | 140 | 305 | 1 | 168 | 8.15e-60 | 188 |
| Msa1450130 | MtrunA17_Chr7g0246541 | 34.122 | 296 | 174 | 5 | 17 | 304 | 13 | 295 | 4.70e-44 | 152 |
| Msa1450130 | MtrunA17_Chr1g0159411 | 32.313 | 294 | 193 | 4 | 12 | 304 | 13 | 301 | 8.51e-41 | 144 |
| Msa1450130 | MtrunA17_Chr4g0019991 | 49.038 | 104 | 53 | 0 | 17 | 120 | 25 | 128 | 8.22e-28 | 104 |
| Msa1450130 | MtrunA17_Chr1g0203491 | 48.624 | 109 | 56 | 0 | 12 | 120 | 21 | 129 | 1.76e-27 | 103 |
| Msa1450130 | MtrunA17_Chr7g0245491 | 30.270 | 185 | 125 | 1 | 120 | 304 | 12 | 192 | 6.78e-25 | 99.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1450130 | AT3G48880.2 | 68.562 | 299 | 92 | 2 | 9 | 305 | 11 | 309 | 1.40e-152 | 429 |
| Msa1450130 | AT3G48880.1 | 68.562 | 299 | 92 | 2 | 9 | 305 | 11 | 309 | 1.40e-152 | 429 |
| Msa1450130 | AT3G48880.3 | 68.562 | 299 | 92 | 2 | 9 | 305 | 11 | 309 | 1.40e-152 | 429 |
| Msa1450130 | AT4G11580.1 | 29.532 | 342 | 188 | 11 | 1 | 303 | 4 | 331 | 8.95e-33 | 124 |
Find 108 sgRNAs with CRISPR-Local
Find 174 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACTGGACATGGATCCT+AGG | 0.531617 | tig0032798:-22216 | Msa1450130:three_prime_UTR |
| AAACTGTTCCTATAAAGTTT+CGG | 0.383190 | tig0032798:+22642 | Msa1450130:intergenic |
| AAATCAAACAAAACAACTCT+TGG | 0.398497 | tig0032798:+24743 | Msa1450130:intergenic |
| AAATTTGAATGATTACAAAT+TGG | 0.309677 | tig0032798:-22783 | Msa1450130:three_prime_UTR |
| AACAGAGAATAACGGCGATC+TGG | 0.478367 | tig0032798:-22870 | Msa1450130:three_prime_UTR |
| AACGACTGGTTCTTCCGGCT+TGG | 0.442940 | tig0032798:-23414 | Msa1450130:CDS |
| AACGGCGATCTGGTATGGTC+TGG | 0.515550 | tig0032798:-22860 | Msa1450130:three_prime_UTR |
| AACGTACAAGAACTGATGAA+GGG | 0.659917 | tig0032798:-22961 | Msa1450130:CDS |
| AAGACGTTGTTTCGAACACG+AGG | 0.632817 | tig0032798:-24181 | Msa1450130:CDS |
| AAGGCATAACATGCAAGGTG+AGG | 0.650786 | tig0032798:-22610 | Msa1450130:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCAAACAAAACAACTCTTG+GGG | + | tig0032798:22319-22338 | Msa0198820:intergenic | 30.0% | |
| ATATCTTGGTCTCTTGAATT+TGG | - | tig0032798:22344-22363 | Msa1450130:three_prime_UTR | 30.0% | |
| CAAGGAAATCTTCAATAATC+GGG | + | tig0032798:23129-23148 | Msa1450130:intergenic | 30.0% | |
| CTAGATTGAAGAATTGTGAT+CGG | - | tig0032798:23385-23404 | Msa1450130:CDS | 30.0% | |
| TTATTAGCACTCCTTCAAAA+TGG | - | tig0032798:23561-23580 | Msa1450130:intron | 30.0% | |
| TGGAACAGAATTAAAAGAAC+AGG | - | tig0032798:23667-23686 | Msa1450130:intron | 30.0% | |
| TTCAGTGAACTCAAGATTAT+GGG | - | tig0032798:23796-23815 | Msa1450130:intron | 30.0% | |
| GCACTTTAAACAGAGAATAA+CGG | - | tig0032798:24183-24202 | Msa1450130:CDS | 30.0% | |
| TTCTTTCACCGAAACTTTAT+AGG | - | tig0032798:24411-24430 | Msa1450130:intron | 30.0% | |
| GTAAATGCTAAAACTCAAGA+AGG | + | tig0032798:24559-24578 | Msa1450130:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0032798 | gene | 22121 | 24962 | 22121 | ID=Msa1450130;Name=Msa1450130 |
| tig0032798 | mRNA | 22121 | 24962 | 22121 | ID=Msa1450130-mRNA-1;Parent=Msa1450130;Name=Msa1450130-mRNA-1;_AED=0.32;_eAED=0.32;_QI=316|1|1|1|0.33|0|4|678|305 |
| tig0032798 | exon | 22121 | 22400 | 22121 | ID=Msa1450130-mRNA-1:exon:17290;Parent=Msa1450130-mRNA-1 |
| tig0032798 | exon | 22494 | 23450 | 22494 | ID=Msa1450130-mRNA-1:exon:17289;Parent=Msa1450130-mRNA-1 |
| tig0032798 | exon | 23849 | 24257 | 23849 | ID=Msa1450130-mRNA-1:exon:17288;Parent=Msa1450130-mRNA-1 |
| tig0032798 | exon | 24697 | 24962 | 24697 | ID=Msa1450130-mRNA-1:exon:17287;Parent=Msa1450130-mRNA-1 |
| tig0032798 | five_prime_UTR | 24697 | 24962 | 24697 | ID=Msa1450130-mRNA-1:five_prime_utr;Parent=Msa1450130-mRNA-1 |
| tig0032798 | five_prime_UTR | 24208 | 24257 | 24208 | ID=Msa1450130-mRNA-1:five_prime_utr;Parent=Msa1450130-mRNA-1 |
| tig0032798 | CDS | 23849 | 24207 | 23849 | ID=Msa1450130-mRNA-1:cds;Parent=Msa1450130-mRNA-1 |
| tig0032798 | CDS | 22892 | 23450 | 22892 | ID=Msa1450130-mRNA-1:cds;Parent=Msa1450130-mRNA-1 |
| tig0032798 | three_prime_UTR | 22494 | 22891 | 22494 | ID=Msa1450130-mRNA-1:three_prime_utr;Parent=Msa1450130-mRNA-1 |
| tig0032798 | three_prime_UTR | 22121 | 22400 | 22121 | ID=Msa1450130-mRNA-1:three_prime_utr;Parent=Msa1450130-mRNA-1 |
| Gene Sequence |
>Msa1450130 ATGGAAGACGTTGTTTCGAACACGAGGAGATGGGAGGATCTGGACACAGATATATTGGTGAAGATTTTTCAGTTGCTTGACATATTCGAGTTAACTTCTGGCATTGCTCATGTTTGTAGCGCATGGCGCATGGCTTGCTGTGATCCACTTCTTTGGAAGACGCTTGATTTGTCAATGTTGAGAAGTAACTTCATCAAGATTCCATTAGAGCCTTTTGTTTACGTTGATGAACGATCTGATAAGAAATTAACCCGATTATTGAAGATTTCCTTGAATCTGAGCAGAATGAGTATAGTGACATTAATCTTCCATTTCAACTTGTACGTGAGTGATGATCAGTTGACATACACTGCTGAAAGGTGCCCACAGCTCAAACGACTGGTTCTTCCGGCTTGGAACAGAATTAAAAGAACAGGAATGTGCAAGGCAATCCGTTGCTGGAAAGAGCTGGAGTCTCTTACAATGCCTAGTATAGCAAACCCGCCATATTTTCTAGAGGAAATTGCAACAAACTGCAAGAACTTCAGTGAACTCAAGATTATGGGGCCATGTGACATCTTCTTTGCTTCAACTCTTGCTGCATTTGTTCCAAAATTGAGAGTCTTGAGCCTTCGGTGTTCAACGCTATATAGGGATGTTCTCATTCTCATCCTAGACAGCTTAGAACATTTAGAAGTGCTCAATATATCACATTGCATTCTAATGGAATGTCTCCCCCCTCCTCAACATAAAAAAATTATCAATGAAATTGATTCTACTATTCGCCAGAAGGCTTCTCGATTGCGCGAATTTATCACATGCATGGATGACTCGTGTATCATGTGCCAACGTACAAGAACTGATGAAGGGATAGTTAGGTGGTACAAGTATGAGGAAGGATTTTGGAAAGAAGATGAGGTGAAGTCCCTTGCACTTTAA |
| Protein sequence |
>Msa1450130 MEDVVSNTRRWEDLDTDILVKIFQLLDIFELTSGIAHVCSAWRMACCDPLLWKTLDLSMLRSNFIKIPLEPFVYVDERSDKKLTRLLKISLNLSRMSIVTLIFHFNLYVSDDQLTYTAERCPQLKRLVLPAWNRIKRTGMCKAIRCWKELESLTMPSIANPPYFLEEIATNCKNFSELKIMGPCDIFFASTLAAFVPKLRVLSLRCSTLYRDVLILILDSLEHLEVLNISHCILMECLPPPQHKKIINEIDSTIRQKASRLREFITCMDDSCIMCQRTRTDEGIVRWYKYEEGFWKEDEVKSLAL* |
