AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0524510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0524510 MtrunA17_Chr3g0145781 91.563 403 27 2 1 403 142 537 0.0 748
Msa0524510 MtrunA17_Chr6g0466611 31.750 400 223 16 13 397 164 528 1.01e-44 164
Msa0524510 MtrunA17_Chr3g0107851 31.593 364 217 12 11 370 137 472 4.34e-41 152
Msa0524510 MtrunA17_Chr4g0062301 28.646 384 222 11 14 386 120 462 1.77e-40 151
Msa0524510 MtrunA17_Chr2g0330401 27.692 390 238 11 1 385 106 456 6.57e-37 140
Msa0524510 MtrunA17_Chr4g0063551 30.769 390 221 13 12 385 161 517 8.36e-37 142
Msa0524510 MtrunA17_Chr2g0307471 29.457 387 220 14 3 378 103 447 1.57e-36 140
Msa0524510 MtrunA17_Chr7g0271121 29.101 378 233 11 1 377 116 459 1.93e-34 135
Msa0524510 MtrunA17_Chr1g0193491 27.895 380 236 9 1 377 124 468 4.02e-34 134
Msa0524510 MtrunA17_Chr2g0293631 27.540 374 232 11 1 372 506 842 9.75e-34 133
Msa0524510 MtrunA17_Chr6g0449911 25.936 374 238 11 1 372 175 511 1.20e-32 130
Msa0524510 MtrunA17_Chr7g0270131 28.267 375 234 12 12 385 253 593 1.31e-32 130
Msa0524510 MtrunA17_Chr7g0247191 27.624 362 219 10 1 361 160 479 2.81e-32 127
Msa0524510 MtrunA17_Chr7g0247181 27.624 362 219 10 1 361 150 469 4.75e-32 127
Msa0524510 MtrunA17_Chr7g0247221 27.624 362 219 10 1 361 153 472 5.08e-32 127
Msa0524510 MtrunA17_Chr3g0098121 29.351 385 228 12 8 387 169 514 1.13e-31 127
Msa0524510 MtrunA17_Chr1g0157691 26.178 382 234 11 1 371 157 501 4.09e-31 125
Msa0524510 MtrunA17_Chr1g0200181 25.916 382 235 11 1 371 155 499 7.13e-31 124
Msa0524510 MtrunA17_Chr1g0176041 28.283 396 240 14 3 386 166 529 2.00e-30 123
Msa0524510 MtrunA17_Chr5g0409981 28.796 382 228 13 12 385 123 468 2.27e-30 122
Msa0524510 MtrunA17_Chr2g0300291 29.243 383 232 12 8 387 158 504 8.31e-30 121
Msa0524510 MtrunA17_Chr4g0040761 29.024 379 209 13 13 379 41 371 7.13e-29 119
Msa0524510 MtrunA17_Chr3g0095031 27.321 377 230 12 16 388 115 451 2.90e-28 116
Msa0524510 MtrunA17_Chr7g0236831 27.869 427 246 15 1 379 173 585 4.02e-28 117
Msa0524510 MtrunA17_Chr5g0442191 27.366 391 242 13 1 388 91 442 4.88e-28 116
Msa0524510 MtrunA17_Chr3g0114081 28.645 391 217 17 16 382 41 393 6.77e-28 115
Msa0524510 MtrunA17_Chr6g0475251 30.141 355 206 12 16 363 128 447 9.60e-28 115
Msa0524510 MtrunA17_Chr4g0034411 28.272 382 220 17 14 384 99 437 5.75e-27 113
Msa0524510 MtrunA17_Chr6g0461751 24.202 376 247 10 1 373 150 490 6.48e-27 112
Msa0524510 MtrunA17_Chr3g0121351 25.510 392 257 11 3 386 157 521 1.20e-26 112
Msa0524510 MtrunA17_Chr2g0277821 25.798 376 241 11 1 373 514 854 1.31e-26 112
Msa0524510 MtrunA17_Chr5g0443991 27.551 392 232 18 3 384 89 438 1.82e-26 112
Msa0524510 MtrunA17_Chr6g0483491 25.405 370 227 9 1 364 20 346 4.53e-26 108
Msa0524510 MtrunA17_Chr2g0310581 26.992 389 237 13 2 384 64 411 3.11e-25 107
Msa0524510 MtrunA17_Chr7g0236841 25.135 370 228 9 1 364 20 346 3.68e-25 106
Msa0524510 MtrunA17_Chr2g0329931 28.150 373 228 12 1 373 51 383 2.38e-24 103
Msa0524510 MtrunA17_Chr2g0297261 25.641 390 238 12 1 384 44 387 2.39e-24 103
Msa0524510 MtrunA17_Chr2g0299681 28.267 375 225 12 1 373 51 383 2.55e-24 103
Msa0524510 MtrunA17_Chr4g0070981 27.614 373 230 11 1 373 51 383 5.89e-24 102
Msa0524510 MtrunA17_Chr7g0263771 25.128 390 240 14 1 384 143 486 9.42e-24 103
Msa0524510 MtrunA17_Chr8g0342861 27.105 380 237 12 1 380 51 390 1.83e-23 101
Msa0524510 MtrunA17_Chr1g0192021 26.750 400 227 13 1 362 36 407 1.43e-22 99.8
Msa0524510 MtrunA17_Chr4g0003441 27.020 396 226 12 1 382 118 464 1.44e-22 99.4
Msa0524510 MtrunA17_Chr4g0058591 28.000 350 203 13 2 343 213 521 2.03e-22 99.4
Msa0524510 MtrunA17_Chr1g0204391 27.446 368 215 10 16 363 69 404 3.28e-22 98.6
Msa0524510 MtrunA17_Chr4g0076491 25.195 385 231 14 1 364 297 645 2.19e-21 96.7
Msa0524510 MtrunA17_Chr1g0149251 27.139 339 208 10 35 371 8 309 3.27e-21 94.0
Msa0524510 MtrunA17_Chr8g0347161 25.474 369 222 12 16 367 127 459 3.61e-21 95.5
Msa0524510 MtrunA17_Chr6g0449741 25.065 383 232 13 1 364 259 605 3.93e-20 92.8
Msa0524510 MtrunA17_Chr8g0347171 25.676 370 220 14 16 367 185 517 4.70e-19 89.4
Msa0524510 MtrunA17_Chr6g0465771 30.247 162 103 3 1 161 102 254 7.53e-15 75.1
Msa0524510 MtrunA17_Chr0c09g0493491 28.824 170 111 3 1 169 102 262 3.18e-13 69.7
Msa0524510 MtrunA17_Chr4g0039471 30.052 193 118 6 1 189 233 412 4.05e-13 70.5
Msa0524510 MtrunA17_Chr4g0062321 24.265 272 154 10 76 337 4 233 5.22e-12 65.5
Msa0524510 MtrunA17_Chr4g0029191 33.117 154 90 5 13 159 67 214 7.28e-12 67.0
Msa0524510 MtrunA17_Chr2g0301281 32.468 154 91 5 13 159 64 211 1.46e-11 66.2
Msa0524510 MtrunA17_Chr8g0364251 30.303 165 96 6 4 156 104 261 2.65e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0524510 AT3G06980.1 66.502 406 122 3 1 403 385 779 0.0 558
Msa0524510 AT4G09730.1 31.605 405 235 11 4 397 126 499 2.15e-50 179
Msa0524510 AT1G55150.1 29.787 376 228 12 1 374 110 451 1.80e-37 142
Msa0524510 AT1G55150.2 29.787 376 228 12 1 374 135 476 3.66e-37 142
Msa0524510 AT5G63120.2 29.442 394 228 14 1 386 176 527 2.34e-35 137
Msa0524510 AT5G14610.7 27.419 372 227 10 5 372 45 377 6.31e-35 135
Msa0524510 AT5G14610.3 27.419 372 227 10 5 372 45 377 6.31e-35 135
Msa0524510 AT5G14610.6 27.128 376 231 10 1 372 172 508 1.08e-34 136
Msa0524510 AT5G14610.2 27.128 376 231 10 1 372 172 508 1.13e-34 135
Msa0524510 AT5G14610.5 27.128 376 231 10 1 372 180 516 1.21e-34 135
Msa0524510 AT3G01540.1 26.984 378 229 12 1 372 169 505 3.56e-34 134
Msa0524510 AT3G01540.4 26.984 378 229 12 1 372 169 505 3.61e-34 134
Msa0524510 AT3G01540.2 26.984 378 229 12 1 372 169 505 3.61e-34 134
Msa0524510 AT3G01540.3 26.984 378 229 12 1 372 169 505 3.61e-34 134
Msa0524510 AT5G14610.1 27.882 373 224 11 5 372 244 576 4.78e-34 134
Msa0524510 AT1G31970.1 30.831 373 213 14 9 372 133 469 9.97e-34 132
Msa0524510 AT4G00660.1 28.177 362 217 10 1 361 142 461 1.06e-33 132
Msa0524510 AT4G00660.2 28.177 362 217 10 1 361 142 461 1.06e-33 132
Msa0524510 AT3G61240.2 27.901 362 218 10 1 361 135 454 3.92e-33 130
Msa0524510 AT3G61240.1 27.901 362 218 10 1 361 135 454 3.92e-33 130
Msa0524510 AT3G06480.1 26.882 372 237 9 1 372 446 782 9.86e-33 131
Msa0524510 AT5G26742.1 29.708 377 230 11 3 377 115 458 1.11e-32 130
Msa0524510 AT5G26742.2 29.708 377 230 11 3 377 115 458 1.11e-32 130
Msa0524510 AT2G42520.2 28.646 384 236 13 12 386 181 535 1.24e-32 130
Msa0524510 AT2G42520.1 28.646 384 236 13 12 386 181 535 1.24e-32 130
Msa0524510 AT2G42520.3 28.646 384 236 13 12 386 181 535 1.24e-32 130
Msa0524510 AT2G45810.1 28.453 362 216 10 1 361 165 484 2.86e-32 128
Msa0524510 AT5G51280.1 26.440 382 233 11 1 371 157 501 5.92e-32 127
Msa0524510 AT5G26742.3 29.589 365 221 12 15 377 35 365 2.05e-31 126
Msa0524510 AT3G18600.1 27.704 379 213 13 14 378 113 444 2.46e-31 125
Msa0524510 AT2G47330.1 27.062 388 244 12 1 385 239 590 2.72e-31 126
Msa0524510 AT5G65900.1 26.992 389 234 12 3 382 167 514 2.84e-31 126
Msa0524510 AT5G60990.1 28.726 369 226 10 1 364 20 356 4.94e-31 124
Msa0524510 AT4G33370.1 26.440 382 233 11 1 371 108 452 1.05e-30 124
Msa0524510 AT1G16280.1 27.366 391 237 13 2 386 70 419 1.89e-30 122
Msa0524510 AT3G58570.1 28.385 384 237 13 12 386 168 522 3.13e-30 123
Msa0524510 AT1G71370.1 27.586 406 220 14 1 382 27 382 6.57e-30 121
Msa0524510 AT1G20920.2 26.064 376 240 12 1 373 202 542 1.19e-29 122
Msa0524510 AT3G22330.1 26.290 407 247 13 1 399 115 476 2.41e-29 120
Msa0524510 AT1G20920.4 25.798 376 241 11 1 373 540 880 5.79e-29 120
Msa0524510 AT1G20920.6 25.798 376 241 11 1 373 540 880 5.79e-29 120
Msa0524510 AT1G20920.3 25.798 376 241 11 1 373 540 880 5.79e-29 120
Msa0524510 AT1G20920.7 25.798 376 241 11 1 373 540 880 5.79e-29 120
Msa0524510 AT1G20920.5 25.798 376 241 11 1 373 540 880 5.79e-29 120
Msa0524510 AT1G20920.1 25.798 376 241 11 1 373 540 880 5.79e-29 120
Msa0524510 AT3G22310.2 26.044 407 248 14 1 399 127 488 1.07e-28 118
Msa0524510 AT3G22310.1 26.044 407 248 14 1 399 127 488 1.13e-28 118
Msa0524510 AT1G77030.1 29.076 368 213 12 13 374 51 376 2.29e-28 118
Msa0524510 AT3G58510.1 27.877 391 240 14 12 390 173 533 2.32e-28 117
Msa0524510 AT3G58510.3 27.877 391 240 14 12 390 173 533 2.32e-28 117
Msa0524510 AT3G58510.2 27.877 391 240 14 12 390 173 533 2.32e-28 117
Msa0524510 AT1G51380.1 26.790 377 228 12 1 373 33 365 2.41e-27 112
Msa0524510 AT5G05450.1 25.815 399 235 12 1 377 27 386 5.08e-27 113
Msa0524510 AT5G54910.1 27.937 383 224 15 14 386 95 435 7.31e-27 113
Msa0524510 AT5G14610.4 26.627 338 204 10 1 333 180 478 1.22e-26 111
Msa0524510 AT3G02065.3 28.415 366 221 13 13 373 133 462 1.47e-26 111
Msa0524510 AT3G02065.2 28.415 366 221 13 13 373 133 462 1.47e-26 111
Msa0524510 AT3G19760.1 25.974 385 243 11 1 384 46 389 2.95e-26 109
Msa0524510 AT3G02065.1 28.650 363 216 14 16 373 1 325 8.01e-26 107
Msa0524510 AT3G09620.2 26.064 376 211 13 1 373 408 719 8.34e-26 110
Msa0524510 AT3G09620.1 26.064 376 211 13 1 373 407 718 8.89e-26 110
Msa0524510 AT5G63120.1 29.412 340 191 13 1 332 176 474 1.93e-25 108
Msa0524510 AT5G63120.3 29.412 340 191 13 1 332 176 474 2.70e-25 107
Msa0524510 AT5G63120.4 29.412 340 191 13 1 332 176 474 2.70e-25 107
Msa0524510 AT5G63120.5 29.412 340 191 13 1 332 176 474 2.70e-25 107
Msa0524510 AT3G06480.2 24.781 343 224 8 1 343 446 754 6.79e-25 107
Msa0524510 AT4G16630.1 27.865 384 235 12 1 380 178 523 8.47e-25 107
Msa0524510 AT1G28180.1 28.571 364 208 14 36 388 19 341 1.39e-24 104
Msa0524510 AT2G33730.1 25.728 412 246 15 1 388 324 699 2.88e-24 105
Msa0524510 AT3G13920.2 27.346 373 231 11 1 373 50 382 1.93e-23 101
Msa0524510 AT3G13920.1 27.346 373 231 11 1 373 50 382 2.19e-23 101
Msa0524510 AT3G13920.3 27.346 373 231 11 1 373 40 372 2.57e-23 101
Msa0524510 AT3G13920.5 27.346 373 231 11 1 373 54 386 2.70e-23 101
Msa0524510 AT1G72730.1 27.128 376 234 11 1 376 52 387 3.03e-23 101
Msa0524510 AT1G54270.1 27.346 373 231 10 1 373 50 382 3.06e-23 100
Msa0524510 AT3G13920.4 28.177 362 220 11 12 373 56 377 1.19e-22 99.4
Msa0524510 AT5G62190.1 26.685 371 198 9 16 363 122 441 1.34e-22 100
Msa0524510 AT1G54270.2 28.177 362 220 10 12 373 56 377 1.42e-22 99.0
Msa0524510 AT5G11170.1 26.343 391 244 13 1 387 57 407 1.47e-22 99.4
Msa0524510 AT5G11200.1 26.343 391 244 13 1 387 57 407 1.47e-22 99.4
Msa0524510 AT3G09720.2 25.128 390 240 14 1 384 152 495 1.54e-22 100
Msa0524510 AT3G09720.1 25.128 390 240 14 1 384 152 495 1.54e-22 100
Msa0524510 AT2G40700.1 25.882 425 225 17 13 371 54 454 1.40e-21 97.4
Msa0524510 AT4G34910.1 24.444 405 232 12 1 362 57 430 5.54e-21 95.5
Msa0524510 AT2G07750.1 24.742 388 242 15 2 374 387 739 7.45e-21 95.5
Msa0524510 AT5G19210.2 25.729 377 235 14 1 361 87 434 8.11e-21 94.4
Msa0524510 AT5G11200.2 24.235 425 238 13 1 371 57 451 1.75e-20 93.6
Msa0524510 AT5G11200.3 25.653 421 250 15 1 387 57 448 1.89e-20 93.6
Msa0524510 AT5G08610.1 25.956 366 224 14 16 367 408 740 3.39e-20 93.6
Msa0524510 AT5G11170.2 26.979 341 206 12 35 371 8 309 4.27e-20 91.3
Msa0524510 AT5G63630.1 25.698 358 235 12 16 367 80 412 1.25e-19 91.3
Msa0524510 AT5G63630.2 25.698 358 235 12 16 367 274 606 2.21e-19 90.9
Msa0524510 AT1G12770.2 23.333 420 275 12 1 388 121 525 7.85e-19 89.0
Msa0524510 AT1G12770.1 23.333 420 275 12 1 388 121 525 7.85e-19 89.0
Msa0524510 AT1G51380.2 25.381 197 135 3 1 197 33 217 3.99e-15 75.5
Msa0524510 AT1G51380.3 25.381 197 135 3 1 197 33 217 2.50e-14 73.6
Msa0524510 AT1G51380.4 25.381 197 135 3 1 197 33 217 2.86e-14 73.6
Msa0524510 AT3G16840.1 30.688 189 113 6 1 174 201 386 4.29e-14 74.7
Msa0524510 AT3G16840.2 30.688 189 113 6 1 174 202 387 4.37e-14 74.7
Msa0524510 AT3G53110.1 25.558 403 234 16 8 390 110 466 4.25e-13 71.2

Find 123 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGAAGTGTCCCAGTTTA+TGG 0.122594 3_4:+77964014 Msa0524510:CDS
ATTGCTGACCAGAGTGGTTC+TGG 0.306260 3_4:+77962093 Msa0524510:CDS
GCCCTGTCCCCTGGCACCTC+TGG 0.308184 3_4:-77964285 None:intergenic
TAGACATTGAACGACAATTA+TGG 0.323737 3_4:-77962251 None:intergenic
TTGGAAAGGGTGCTCTCAAC+TGG 0.323947 3_4:+77964456 Msa0524510:three_prime_UTR
ATAAACTGGGACACTTCTTT+TGG 0.327753 3_4:-77964012 None:intergenic
TGTCCGTGCAAACCATAAAC+TGG 0.332471 3_4:-77964026 None:intergenic
TTGCAGAACACAATAGTTCT+TGG 0.346234 3_4:-77963747 None:intergenic
TCATTTGCTCCTGTTATTGC+CGG 0.349281 3_4:+77962057 Msa0524510:CDS
GATGAGGTTGATATCCTCTT+TGG 0.353589 3_4:+77962599 Msa0524510:CDS
GGTAGCACCGACAATATTCT+AGG 0.364067 3_4:+77964535 Msa0524510:three_prime_UTR
GGCGAGTTAAAGAGAGGTTA+TGG 0.368794 3_4:-77962014 None:intergenic
CAAGACTTTCCGGCAATAAC+AGG 0.372706 3_4:-77962066 None:intergenic
AAAAGAGAAGAGAATCAAAA+TGG 0.386747 3_4:+77961593 Msa0524510:five_prime_UTR
AGCAATTATACAAGACTTTC+CGG 0.389114 3_4:-77962076 None:intergenic
GGCAAAGGAAGGAAATCTTT+TGG 0.389304 3_4:+77964514 Msa0524510:three_prime_UTR
AGCCAGAGGTGCCAGGGGAC+AGG 0.398550 3_4:+77964283 Msa0524510:CDS
GTCGATAAAACCACGAGTTT+CGG 0.403323 3_4:+77961751 Msa0524510:five_prime_UTR
CACAGCTGAGTTAGCTTCTC+AGG 0.409704 3_4:+77962224 Msa0524510:CDS
TGTCGTTCAATGTCTAAATC+TGG 0.417609 3_4:+77962258 Msa0524510:CDS
GAGTTGGATGGGGAGACAAT+AGG 0.426703 3_4:+77961905 Msa0524510:five_prime_UTR
AAGGCATTTATCTTTGTTGT+TGG 0.426934 3_4:+77964308 Msa0524510:CDS
CAGAGTGTTTAGTGGGTCTC+TGG 0.429990 3_4:-77961678 None:intergenic
TGCAGAACACAATAGTTCTT+GGG 0.438418 3_4:-77963746 None:intergenic
CCCATGACCATTTCACAATC+AGG 0.439831 3_4:-77962742 None:intergenic
GAGCTGAAGAAGTAGGAAGT+TGG 0.443767 3_4:+77964419 Msa0524510:three_prime_UTR
TCCACTGCATGATGTACCTT+CGG 0.449294 3_4:+77964382 Msa0524510:CDS
GAGAGATTCAATCATGAAAT+CGG 0.449692 3_4:-77961992 None:intergenic
AGTTGGATGGGGAGACAATA+GGG 0.452318 3_4:+77961906 Msa0524510:five_prime_UTR
GAAGAACAGCCAGAGGTGCC+AGG 0.461221 3_4:+77964276 Msa0524510:CDS
CTAAAATCATTTAAAGACAT+TGG 0.461700 3_4:+77961964 Msa0524510:five_prime_UTR
AACTCGCCCTTCCAACATAC+AGG 0.470926 3_4:+77962029 Msa0524510:CDS
AACTCATTCGAGAGTTGGAT+GGG 0.471462 3_4:+77961894 Msa0524510:five_prime_UTR
AAATGCCTTGCCCTGTCCCC+TGG 0.473330 3_4:-77964294 None:intergenic
TGTCTGAGACGTTGAATGAT+TGG 0.476607 3_4:-77962138 None:intergenic
AGTTTATGGTTTGCACGGAC+AGG 0.484625 3_4:+77964028 Msa0524510:CDS
TTGGTGTGTGCAGGCCATGC+TGG 0.485623 3_4:+77963491 Msa0524510:intron
AACTTGTAACAGAGCTTCGC+GGG 0.488229 3_4:+77964175 Msa0524510:intron
AGGTGCCAGGGGACAGGGCA+AGG 0.488567 3_4:+77964289 Msa0524510:CDS
GAATTCCCTTTCTATCAAAA+CGG 0.499892 3_4:-77963909 None:intergenic
GCCAGAGGTGCCAGGGGACA+GGG 0.502782 3_4:+77964284 Msa0524510:CDS
ATTATGTTGCAGTGCTGTCT+TGG 0.503149 3_4:+77962577 Msa0524510:intron
TTGCTGTTTAGCATCGGAGT+TGG 0.503766 3_4:+77961648 Msa0524510:five_prime_UTR
TCCTGATTGTGAAATGGTCA+TGG 0.506213 3_4:+77962741 Msa0524510:CDS
GTAGATTGTAGTGGGGAAGA+TGG 0.506554 3_4:+77963648 Msa0524510:CDS
AGCTGCTCTTCTGCAGCTTG+CGG 0.507830 3_4:+77963710 Msa0524510:CDS
TTTAATTGATTGCTTGGAAA+GGG 0.510457 3_4:+77964443 Msa0524510:three_prime_UTR
TCATCGACCTAGAATATTGT+CGG 0.512080 3_4:-77964542 None:intergenic
TGGCGATGAGGACTTCGAAA+AGG 0.514933 3_4:+77962619 Msa0524510:CDS
AGATAATAGTAGATTGTAGT+GGG 0.515530 3_4:+77963640 Msa0524510:CDS
ATGCTTGATTAGTTCTTCAC+CGG 0.515910 3_4:+77962649 Msa0524510:CDS
CAACTCATTCGAGAGTTGGA+TGG 0.516133 3_4:+77961893 Msa0524510:five_prime_UTR
GGTTGATGAGGAAGAGAAAA+CGG 0.523534 3_4:-77962383 None:intergenic
ACTGCAACTCATTCGAGAGT+TGG 0.528094 3_4:+77961889 Msa0524510:five_prime_UTR
GAAGAAGACAGAGTGTTTAG+TGG 0.529879 3_4:-77961686 None:intergenic
CAATAGGGTTAGCAGTCAAT+CGG 0.532781 3_4:+77961921 Msa0524510:five_prime_UTR
AGTGAATATGTACGGCGTGT+TGG 0.534281 3_4:+77964254 Msa0524510:CDS
CCTGATTGTGAAATGGTCAT+GGG 0.535391 3_4:+77962742 Msa0524510:CDS
GTCCCAGTTTATGGTTTGCA+CGG 0.536407 3_4:+77964023 Msa0524510:CDS
GTGACAAGCATAGATTTGAA+TGG 0.537448 3_4:-77962288 None:intergenic
AAATCTATGCTTGTCACTGG+TGG 0.541886 3_4:+77962294 Msa0524510:CDS
AAGAAGACAGAGTGTTTAGT+GGG 0.547041 3_4:-77961685 None:intergenic
ATCACGGCTTACTAGTATGA+AGG 0.552151 3_4:+77963972 Msa0524510:CDS
TTGCAGTGCTGTCTTGGATG+AGG 0.552233 3_4:+77962583 Msa0524510:intron
CGAAGTCCTCATCGCCAAAG+AGG 0.554253 3_4:-77962613 None:intergenic
CAGATAATAGTAGATTGTAG+TGG 0.557322 3_4:+77963639 Msa0524510:CDS
TTCAAATCTATGCTTGTCAC+TGG 0.558308 3_4:+77962291 Msa0524510:CDS
GTGGTTGCGCAAGGTGTTTG+TGG 0.559691 3_4:+77961618 Msa0524510:five_prime_UTR
GTATTGAGAGCTGAAGAAGT+AGG 0.560738 3_4:+77964412 Msa0524510:exon
TGGAAGGGCGAGTTAAAGAG+AGG 0.560834 3_4:-77962020 None:intergenic
AAATGACATGGCCTGTATGT+TGG 0.565806 3_4:-77962040 None:intergenic
AGTAATGGAAAGAAATCGGA+AGG 0.566217 3_4:+77964355 Msa0524510:CDS
TCGATGAAACGTGCATTGCT+AGG 0.568808 3_4:+77964558 Msa0524510:three_prime_UTR
AAGAACAGCCAGAGGTGCCA+GGG 0.570215 3_4:+77964277 Msa0524510:CDS
AATAAATATTGTGTGTCCAC+CGG 0.571880 3_4:-77962668 None:intergenic
CAACTTGTAACAGAGCTTCG+CGG 0.572338 3_4:+77964174 Msa0524510:intron
TGGAAAGGGTGCTCTCAACT+GGG 0.579482 3_4:+77964457 Msa0524510:three_prime_UTR
AAGTCTAACATGCATGGCAA+AGG 0.579671 3_4:+77964499 Msa0524510:three_prime_UTR
CTTGATTAGTTCTTCACCGG+TGG 0.581413 3_4:+77962652 Msa0524510:CDS
AAAGCGTTGCTGTTTAGCAT+CGG 0.582268 3_4:+77961642 Msa0524510:five_prime_UTR
TGTGTCATGGCAGCATGGAA+AGG 0.582945 3_4:-77963946 None:intergenic
GGTGCGAGTATAACAAGAGT+AGG 0.587052 3_4:-77962201 None:intergenic
CTCAGACAACAAGAACTACA+AGG 0.588339 3_4:+77962153 Msa0524510:CDS
TGATATCCTCTTTGGCGATG+AGG 0.588829 3_4:+77962607 Msa0524510:CDS
CTAACATGCATGGCAAAGGA+AGG 0.589079 3_4:+77964503 Msa0524510:three_prime_UTR
TGCACTTAACTAATCTAACA+TGG 0.590356 3_4:+77962415 Msa0524510:CDS
ATGTCTTTCCAGAACCACTC+TGG 0.590455 3_4:-77962101 None:intergenic
CTGAGAAGCTAACTCAGCTG+TGG 0.591686 3_4:-77962223 None:intergenic
TTTCTCTTCCTCATCAACCA+AGG 0.592789 3_4:+77962387 Msa0524510:CDS
AAGCCGTGATTCTTGTGTCA+TGG 0.593997 3_4:-77963959 None:intergenic
TATTGTAAGTCTAACATGCA+TGG 0.594332 3_4:+77964493 Msa0524510:three_prime_UTR
ACACGCCGTACATATTCACT+TGG 0.594509 3_4:-77964251 None:intergenic
GGGAATTGACTTCACTAGAG+TGG 0.597817 3_4:+77964196 Msa0524510:CDS
TCAACTCGACACTTTAAACC+AGG 0.598198 3_4:+77962332 Msa0524510:CDS
GTCCGTGCAAACCATAAACT+GGG 0.602781 3_4:-77964025 None:intergenic
ACATATTCACTTGGATCACG+TGG 0.605024 3_4:-77964242 None:intergenic
GTGATCCAAGTGAATATGTA+CGG 0.607674 3_4:+77964246 Msa0524510:CDS
TTCAATGTCTAAATCTGGCA+AGG 0.610738 3_4:+77962263 Msa0524510:CDS
ATGAGGAAGAGAAAACGGCC+AGG 0.612771 3_4:-77962378 None:intergenic
TGAGAAGCTAACTCAGCTGT+GGG 0.613832 3_4:-77962222 None:intergenic
AAAATGGCGGTGGTTGCGCA+AGG 0.615353 3_4:+77961609 Msa0524510:five_prime_UTR
GAAGAGAATCAAAATGGCGG+TGG 0.615716 3_4:+77961599 Msa0524510:five_prime_UTR
GACATGGCCTGTATGTTGGA+AGG 0.625996 3_4:-77962036 None:intergenic
GATGTCTTCATAGCTACACC+TGG 0.633711 3_4:+77962360 Msa0524510:CDS
CTGCCATGACACAAGAATCA+CGG 0.635761 3_4:+77963956 Msa0524510:CDS
GATAATAGTAGATTGTAGTG+GGG 0.637024 3_4:+77963641 Msa0524510:CDS
AGAACAGCCAGAGGTGCCAG+GGG 0.638006 3_4:+77964278 Msa0524510:CDS
GCCGAAGGTACATCATGCAG+TGG 0.640301 3_4:-77964383 None:intergenic
TGTATAATTGCTGACCAGAG+TGG 0.648838 3_4:+77962087 Msa0524510:CDS
TCTCAATACTCATAAGCCGA+AGG 0.655130 3_4:-77964398 None:intergenic
ACATGGCCTGTATGTTGGAA+GGG 0.662222 3_4:-77962035 None:intergenic
TAGGGTTAGCAGTCAATCGG+AGG 0.663524 3_4:+77961924 Msa0524510:five_prime_UTR
ATTCTTGTGTCATGGCAGCA+TGG 0.663708 3_4:-77963951 None:intergenic
ACTCATTCGAGAGTTGGATG+GGG 0.667637 3_4:+77961895 Msa0524510:five_prime_UTR
CGTGTTGGAAGAACAGCCAG+AGG 0.669248 3_4:+77964269 Msa0524510:CDS
ACTGGTCACTGAATCCAGCA+TGG 0.671798 3_4:-77963505 None:intergenic
CAACTCGACACTTTAAACCA+GGG 0.695695 3_4:+77962333 Msa0524510:CDS
GTAATGGAAAGAAATCGGAA+GGG 0.700702 3_4:+77964356 Msa0524510:CDS
AATAACAGGAGCAAATGACA+TGG 0.700797 3_4:-77962052 None:intergenic
ACTTGTAACAGAGCTTCGCG+GGG 0.711965 3_4:+77964176 Msa0524510:intron
CATATTCACTTGGATCACGT+GGG 0.715544 3_4:-77964241 None:intergenic
AGAGAAGAGAATCAAAATGG+CGG 0.724812 3_4:+77961596 Msa0524510:five_prime_UTR
CTATGAAGACATCAACACCC+TGG 0.726139 3_4:-77962350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCATAATATGAAATAAA+AGG + chr3_4:77963226-77963245 Msa0524510:intron 10.0%
!! ATTCATAATATGAAATAAAA+GGG + chr3_4:77963227-77963246 Msa0524510:intron 10.0%
!!! AATATTAATTTGAAATTAAG+TGG - chr3_4:77963536-77963555 None:intergenic 10.0%
!! GTTAAGATAAATTAAATCAT+TGG - chr3_4:77962547-77962566 None:intergenic 15.0%
!!! GTTATTGTTTTACTATTTAT+AGG + chr3_4:77963298-77963317 Msa0524510:intron 15.0%
!! TAATATATTCTTAAATTGCT+GGG + chr3_4:77963463-77963482 Msa0524510:intron 15.0%
!!! CTTTTTGTTACTTTTTATTT+TGG + chr3_4:77963825-77963844 Msa0524510:intron 15.0%
!!! TTTCAATTTTCAACAAGATA+AGG - chr3_4:77961562-77961581 None:intergenic 20.0%
!! CTAAAATCATTTAAAGACAT+TGG + chr3_4:77961964-77961983 Msa0524510:five_prime_UTR 20.0%
!! AGAAATCAATTTATAACACA+TGG - chr3_4:77962511-77962530 None:intergenic 20.0%
!!! CTTTCTATTTTATTCATCTT+AGG + chr3_4:77962829-77962848 Msa0524510:intron 20.0%
!! ATTCAATTTAAATCTCATGT+GGG - chr3_4:77962943-77962962 None:intergenic 20.0%
!! AATTCAATTTAAATCTCATG+TGG - chr3_4:77962944-77962963 None:intergenic 20.0%
!! ATACACAATGATATGTATAA+AGG - chr3_4:77962982-77963001 None:intergenic 20.0%
!! TTTATACATATCATTGTGTA+TGG + chr3_4:77962981-77963000 Msa0524510:intron 20.0%
!! TCCAGTATTAAATTTATTAG+TGG - chr3_4:77963145-77963164 None:intergenic 20.0%
!!! GTATTTCTTTTTGAATAGTA+TGG - chr3_4:77963170-77963189 None:intergenic 20.0%
!!! GAAATTTTGAGAAATGTTTA+GGG - chr3_4:77963200-77963219 None:intergenic 20.0%
!!! TGAAATTTTGAGAAATGTTT+AGG - chr3_4:77963201-77963220 None:intergenic 20.0%
!! TTGTACTAGTTGTATATAAT+TGG - chr3_4:77963278-77963297 None:intergenic 20.0%
!! GCAATTTAAGAATATATTAC+AGG - chr3_4:77963462-77963481 None:intergenic 20.0%
!! GTAATATATTCTTAAATTGC+TGG + chr3_4:77963462-77963481 Msa0524510:intron 20.0%
!! AATATATTCTTAAATTGCTG+GGG + chr3_4:77963464-77963483 Msa0524510:intron 20.0%
!!! AAAAAGTAATGGAAAGAAAT+CGG + chr3_4:77964351-77964370 Msa0524510:CDS 20.0%
! AAAAGAGAAGAGAATCAAAA+TGG + chr3_4:77961593-77961612 Msa0524510:five_prime_UTR 25.0%
!! TGATTTTAGACTGAAAAAGT+TGG - chr3_4:77961953-77961972 None:intergenic 25.0%
! TTAACTAATCTAACATGGTA+TGG + chr3_4:77962420-77962439 Msa0524510:intron 25.0%
! AAAATGATAGGTGATAGAAA+AGG - chr3_4:77962457-77962476 None:intergenic 25.0%
! GATAAATTAAATCATTGGTG+CGG - chr3_4:77962542-77962561 None:intergenic 25.0%
!!! AGTTTGCTGTAAACATTTTT+TGG - chr3_4:77962710-77962729 None:intergenic 25.0%
! AAAAAATGTTTACAGCAAAC+TGG + chr3_4:77962709-77962728 Msa0524510:CDS 25.0%
!!! GTTGGTAACAATTATGAAAA+TGG + chr3_4:77963017-77963036 Msa0524510:intron 25.0%
! GCCACTAATAAATTTAATAC+TGG + chr3_4:77963141-77963160 Msa0524510:intron 25.0%
! AGATAATAGTAGATTGTAGT+GGG + chr3_4:77963640-77963659 Msa0524510:CDS 25.0%
!!! ATTAAACCGTTTTGATAGAA+AGG + chr3_4:77963903-77963922 Msa0524510:CDS 25.0%
!!! TTAAACCGTTTTGATAGAAA+GGG + chr3_4:77963904-77963923 Msa0524510:CDS 25.0%
! GTGTTACAAATAATTCATTG+TGG + chr3_4:77964151-77964170 Msa0524510:intron 25.0%
!!! TTTTAATTGATTGCTTGGAA+AGG + chr3_4:77964442-77964461 Msa0524510:three_prime_UTR 25.0%
! TTTAATTGATTGCTTGGAAA+GGG + chr3_4:77964443-77964462 Msa0524510:three_prime_UTR 25.0%
GAGAGATTCAATCATGAAAT+CGG - chr3_4:77961995-77962014 None:intergenic 30.0%
AGCAATTATACAAGACTTTC+CGG - chr3_4:77962079-77962098 None:intergenic 30.0%
TAGACATTGAACGACAATTA+TGG - chr3_4:77962254-77962273 None:intergenic 30.0%
TGCACTTAACTAATCTAACA+TGG + chr3_4:77962415-77962434 Msa0524510:CDS 30.0%
GGTTTAGTCAACAAAATGAT+AGG - chr3_4:77962469-77962488 None:intergenic 30.0%
AATAAATATTGTGTGTCCAC+CGG - chr3_4:77962671-77962690 None:intergenic 30.0%
! AGTTTTTCCTGATTGTGAAA+TGG + chr3_4:77962735-77962754 Msa0524510:CDS 30.0%
CTGTGATATGAGAAAACATT+TGG + chr3_4:77962901-77962920 Msa0524510:intron 30.0%
AGGGATAGTAAAATCGAAAA+TGG + chr3_4:77963246-77963265 Msa0524510:intron 30.0%
TGAAATTAAGTGGTCATTAC+TGG - chr3_4:77963526-77963545 None:intergenic 30.0%
CAGATAATAGTAGATTGTAG+TGG + chr3_4:77963639-77963658 Msa0524510:CDS 30.0%
GATAATAGTAGATTGTAGTG+GGG + chr3_4:77963641-77963660 Msa0524510:CDS 30.0%
GAATTCCCTTTCTATCAAAA+CGG - chr3_4:77963912-77963931 None:intergenic 30.0%
! AAGGCATTTATCTTTGTTGT+TGG + chr3_4:77964308-77964327 Msa0524510:CDS 30.0%
!!! TTCCATTACTTTTTGTGCAA+GGG - chr3_4:77964345-77964364 None:intergenic 30.0%
!!! TTTCCATTACTTTTTGTGCA+AGG - chr3_4:77964346-77964365 None:intergenic 30.0%
!!! GTTGGTTTTAATTGATTGCT+TGG + chr3_4:77964437-77964456 Msa0524510:three_prime_UTR 30.0%
TATTGTAAGTCTAACATGCA+TGG + chr3_4:77964493-77964512 Msa0524510:three_prime_UTR 30.0%
AGAGAAGAGAATCAAAATGG+CGG + chr3_4:77961596-77961615 Msa0524510:five_prime_UTR 35.0%
! AAGAAGACAGAGTGTTTAGT+GGG - chr3_4:77961688-77961707 None:intergenic 35.0%
!! GGCGGATTTTGAATTTGAAT+TGG - chr3_4:77961731-77961750 None:intergenic 35.0%
! TTGTAGTTGTTTGCGTTTTG+TGG - chr3_4:77961830-77961849 None:intergenic 35.0%
AATAACAGGAGCAAATGACA+TGG - chr3_4:77962055-77962074 None:intergenic 35.0%
TGTCGTTCAATGTCTAAATC+TGG + chr3_4:77962258-77962277 Msa0524510:CDS 35.0%
TTCAATGTCTAAATCTGGCA+AGG + chr3_4:77962263-77962282 Msa0524510:CDS 35.0%
GTGACAAGCATAGATTTGAA+TGG - chr3_4:77962291-77962310 None:intergenic 35.0%
TTCAAATCTATGCTTGTCAC+TGG + chr3_4:77962291-77962310 Msa0524510:CDS 35.0%
!!! TGTCGAGTTGAGTTTTTTGT+CGG - chr3_4:77962323-77962342 None:intergenic 35.0%
TAGTTAAGTGCAAAAAGCCT+TGG - chr3_4:77962407-77962426 None:intergenic 35.0%
GGTAAATGACAAGTGTATTC+AGG - chr3_4:77962490-77962509 None:intergenic 35.0%
! ATGCTTGATTAGTTCTTCAC+CGG + chr3_4:77962649-77962668 Msa0524510:CDS 35.0%
GAACTTATTCGATGCATACT+AGG - chr3_4:77962769-77962788 None:intergenic 35.0%
AACGTATTCCCTAAATTTGC+TGG + chr3_4:77962866-77962885 Msa0524510:intron 35.0%
TACTATTTATAGGTCCGCTT+TGG + chr3_4:77963308-77963327 Msa0524510:intron 35.0%
!! CTTAAATTGCTGGGGTATTT+TGG + chr3_4:77963472-77963491 Msa0524510:intron 35.0%
TGCAGAACACAATAGTTCTT+GGG - chr3_4:77963749-77963768 None:intergenic 35.0%
TTGCAGAACACAATAGTTCT+TGG - chr3_4:77963750-77963769 None:intergenic 35.0%
! ACAAAGTATTCTGAACTCTC+AGG + chr3_4:77963769-77963788 Msa0524510:intron 35.0%
! ATAAACTGGGACACTTCTTT+TGG - chr3_4:77964015-77964034 None:intergenic 35.0%
!! AAAAGAAGTGTCCCAGTTTA+TGG + chr3_4:77964014-77964033 Msa0524510:CDS 35.0%
!! TATTCATCTGTTTTTCTCGC+TGG + chr3_4:77964051-77964070 Msa0524510:intron 35.0%
GTGATCCAAGTGAATATGTA+CGG + chr3_4:77964246-77964265 Msa0524510:CDS 35.0%
ATCCCTTGCACAAAAAGTAA+TGG + chr3_4:77964340-77964359 Msa0524510:CDS 35.0%
! AGTAATGGAAAGAAATCGGA+AGG + chr3_4:77964355-77964374 Msa0524510:CDS 35.0%
! GTAATGGAAAGAAATCGGAA+GGG + chr3_4:77964356-77964375 Msa0524510:CDS 35.0%
TCATCGACCTAGAATATTGT+CGG - chr3_4:77964545-77964564 None:intergenic 35.0%
!! AAAGCGTTGCTGTTTAGCAT+CGG + chr3_4:77961642-77961661 Msa0524510:five_prime_UTR 40.0%
! GAAGAAGACAGAGTGTTTAG+TGG - chr3_4:77961689-77961708 None:intergenic 40.0%
!!! GAAACTCGTGGTTTTATCGA+CGG - chr3_4:77961752-77961771 None:intergenic 40.0%
GTCGATAAAACCACGAGTTT+CGG + chr3_4:77961751-77961770 Msa0524510:five_prime_UTR 40.0%
!! TTTTAGTCTTCCGAAACTCG+TGG - chr3_4:77961764-77961783 None:intergenic 40.0%
!!! TGGTATTTTGCTTGGTTCTG+TGG - chr3_4:77961810-77961829 None:intergenic 40.0%
! GCGTTTTGTGGTATTTTGCT+TGG - chr3_4:77961818-77961837 None:intergenic 40.0%
AACTCATTCGAGAGTTGGAT+GGG + chr3_4:77961894-77961913 Msa0524510:five_prime_UTR 40.0%
! CAATAGGGTTAGCAGTCAAT+CGG + chr3_4:77961921-77961940 Msa0524510:five_prime_UTR 40.0%
AAATGACATGGCCTGTATGT+TGG - chr3_4:77962043-77962062 None:intergenic 40.0%
TCATTTGCTCCTGTTATTGC+CGG + chr3_4:77962057-77962076 Msa0524510:CDS 40.0%
TGTATAATTGCTGACCAGAG+TGG + chr3_4:77962087-77962106 Msa0524510:CDS 40.0%
! TGTCTGAGACGTTGAATGAT+TGG - chr3_4:77962141-77962160 None:intergenic 40.0%
CTCAGACAACAAGAACTACA+AGG + chr3_4:77962153-77962172 Msa0524510:CDS 40.0%
AAATCTATGCTTGTCACTGG+TGG + chr3_4:77962294-77962313 Msa0524510:CDS 40.0%
TCAACTCGACACTTTAAACC+AGG + chr3_4:77962332-77962351 Msa0524510:CDS 40.0%
CAACTCGACACTTTAAACCA+GGG + chr3_4:77962333-77962352 Msa0524510:CDS 40.0%
!! GGTTGATGAGGAAGAGAAAA+CGG - chr3_4:77962386-77962405 None:intergenic 40.0%
TTTCTCTTCCTCATCAACCA+AGG + chr3_4:77962387-77962406 Msa0524510:CDS 40.0%
ATTATGTTGCAGTGCTGTCT+TGG + chr3_4:77962577-77962596 Msa0524510:intron 40.0%
!! GATGAGGTTGATATCCTCTT+TGG + chr3_4:77962599-77962618 Msa0524510:CDS 40.0%
TCCTGATTGTGAAATGGTCA+TGG + chr3_4:77962741-77962760 Msa0524510:CDS 40.0%
CCTGATTGTGAAATGGTCAT+GGG + chr3_4:77962742-77962761 Msa0524510:CDS 40.0%
AATAAGTTCTCGCCTTGAAG+AGG + chr3_4:77962780-77962799 Msa0524510:intron 40.0%
!! TATTCCCTAAATTTGCTGGC+TGG + chr3_4:77962870-77962889 Msa0524510:intron 40.0%
!! ATTCCCTAAATTTGCTGGCT+GGG + chr3_4:77962871-77962890 Msa0524510:intron 40.0%
AATCTCATGTGGGAGACAAA+AGG - chr3_4:77962933-77962952 None:intergenic 40.0%
CATATCATTGTGTATGGCAG+TGG + chr3_4:77962987-77963006 Msa0524510:intron 40.0%
GGTGCAGTCAACTCAATAAT+TGG + chr3_4:77963082-77963101 Msa0524510:intron 40.0%
AGTCAACTCAATAATTGGCG+CGG + chr3_4:77963087-77963106 Msa0524510:intron 40.0%
ATAATCAGCTGCTGTGTAAG+TGG + chr3_4:77963590-77963609 Msa0524510:intron 40.0%
TAATCAGCTGCTGTGTAAGT+GGG + chr3_4:77963591-77963610 Msa0524510:intron 40.0%
ATCACGGCTTACTAGTATGA+AGG + chr3_4:77963972-77963991 Msa0524510:CDS 40.0%
CATATTCACTTGGATCACGT+GGG - chr3_4:77964244-77964263 None:intergenic 40.0%
ACATATTCACTTGGATCACG+TGG - chr3_4:77964245-77964264 None:intergenic 40.0%
TCTCAATACTCATAAGCCGA+AGG - chr3_4:77964401-77964420 None:intergenic 40.0%
GTATTGAGAGCTGAAGAAGT+AGG + chr3_4:77964412-77964431 Msa0524510:exon 40.0%
AAGTCTAACATGCATGGCAA+AGG + chr3_4:77964499-77964518 Msa0524510:three_prime_UTR 40.0%
! GGCAAAGGAAGGAAATCTTT+TGG + chr3_4:77964514-77964533 Msa0524510:three_prime_UTR 40.0%
GAAGAGAATCAAAATGGCGG+TGG + chr3_4:77961599-77961618 Msa0524510:five_prime_UTR 45.0%
!! TTGCTGTTTAGCATCGGAGT+TGG + chr3_4:77961648-77961667 Msa0524510:five_prime_UTR 45.0%
ACTGCAACTCATTCGAGAGT+TGG + chr3_4:77961889-77961908 Msa0524510:five_prime_UTR 45.0%
CAACTCATTCGAGAGTTGGA+TGG + chr3_4:77961893-77961912 Msa0524510:five_prime_UTR 45.0%
ACTCATTCGAGAGTTGGATG+GGG + chr3_4:77961895-77961914 Msa0524510:five_prime_UTR 45.0%
AGTTGGATGGGGAGACAATA+GGG + chr3_4:77961906-77961925 Msa0524510:five_prime_UTR 45.0%
GGCGAGTTAAAGAGAGGTTA+TGG - chr3_4:77962017-77962036 None:intergenic 45.0%
ACATGGCCTGTATGTTGGAA+GGG - chr3_4:77962038-77962057 None:intergenic 45.0%
CAAGACTTTCCGGCAATAAC+AGG - chr3_4:77962069-77962088 None:intergenic 45.0%
ATGTCTTTCCAGAACCACTC+TGG - chr3_4:77962104-77962123 None:intergenic 45.0%
GGTGCGAGTATAACAAGAGT+AGG - chr3_4:77962204-77962223 None:intergenic 45.0%
TGAGAAGCTAACTCAGCTGT+GGG - chr3_4:77962225-77962244 None:intergenic 45.0%
CTATGAAGACATCAACACCC+TGG - chr3_4:77962353-77962372 None:intergenic 45.0%
GATGTCTTCATAGCTACACC+TGG + chr3_4:77962360-77962379 Msa0524510:CDS 45.0%
! GCAAAAAGCCTTGGTTGATG+AGG - chr3_4:77962398-77962417 None:intergenic 45.0%
TGATATCCTCTTTGGCGATG+AGG + chr3_4:77962607-77962626 Msa0524510:CDS 45.0%
! CTTGATTAGTTCTTCACCGG+TGG + chr3_4:77962652-77962671 Msa0524510:CDS 45.0%
CCCATGACCATTTCACAATC+AGG - chr3_4:77962745-77962764 None:intergenic 45.0%
AGTGAAACCTGACCTCTTCA+AGG - chr3_4:77962795-77962814 None:intergenic 45.0%
AAGCCCAGCCAGCAAATTTA+GGG - chr3_4:77962877-77962896 None:intergenic 45.0%
ACCTGTCTCTGTTGTCAATG+CGG + chr3_4:77963048-77963067 Msa0524510:intron 45.0%
CTGTTGTCAATGCGGAAATG+CGG + chr3_4:77963056-77963075 Msa0524510:intron 45.0%
AAAGTTGAAAGCGGCCAAAG+CGG - chr3_4:77963325-77963344 None:intergenic 45.0%
CGCGTGTCAAAAGTTGAAAG+CGG - chr3_4:77963334-77963353 None:intergenic 45.0%
! GTAGATTGTAGTGGGGAAGA+TGG + chr3_4:77963648-77963667 Msa0524510:CDS 45.0%
ATTCTTGTGTCATGGCAGCA+TGG - chr3_4:77963954-77963973 None:intergenic 45.0%
CTGCCATGACACAAGAATCA+CGG + chr3_4:77963956-77963975 Msa0524510:CDS 45.0%
AAGCCGTGATTCTTGTGTCA+TGG - chr3_4:77963962-77963981 None:intergenic 45.0%
! AACTGGGACACTTCTTTTGG+TGG - chr3_4:77964012-77964031 None:intergenic 45.0%
! GTCCCAGTTTATGGTTTGCA+CGG + chr3_4:77964023-77964042 Msa0524510:CDS 45.0%
GTCCGTGCAAACCATAAACT+GGG - chr3_4:77964028-77964047 None:intergenic 45.0%
TGTCCGTGCAAACCATAAAC+TGG - chr3_4:77964029-77964048 None:intergenic 45.0%
! AGTTTATGGTTTGCACGGAC+AGG + chr3_4:77964028-77964047 Msa0524510:CDS 45.0%
CAACTTGTAACAGAGCTTCG+CGG + chr3_4:77964174-77964193 Msa0524510:intron 45.0%
AACTTGTAACAGAGCTTCGC+GGG + chr3_4:77964175-77964194 Msa0524510:intron 45.0%
! GGGAATTGACTTCACTAGAG+TGG + chr3_4:77964196-77964215 Msa0524510:CDS 45.0%
ACACGCCGTACATATTCACT+TGG - chr3_4:77964254-77964273 None:intergenic 45.0%
AGTGAATATGTACGGCGTGT+TGG + chr3_4:77964254-77964273 Msa0524510:CDS 45.0%
TCCACTGCATGATGTACCTT+CGG + chr3_4:77964382-77964401 Msa0524510:CDS 45.0%
GAGCTGAAGAAGTAGGAAGT+TGG + chr3_4:77964419-77964438 Msa0524510:three_prime_UTR 45.0%
CTAACATGCATGGCAAAGGA+AGG + chr3_4:77964503-77964522 Msa0524510:three_prime_UTR 45.0%
!! GGTAGCACCGACAATATTCT+AGG + chr3_4:77964535-77964554 Msa0524510:three_prime_UTR 45.0%
TCGATGAAACGTGCATTGCT+AGG + chr3_4:77964558-77964577 Msa0524510:three_prime_UTR 45.0%
! CAGAGTGTTTAGTGGGTCTC+TGG - chr3_4:77961681-77961700 None:intergenic 50.0%
!!! ACTCGTGGTTTTATCGACGG+CGG - chr3_4:77961749-77961768 None:intergenic 50.0%
GAGTTGGATGGGGAGACAAT+AGG + chr3_4:77961905-77961924 Msa0524510:five_prime_UTR 50.0%
! TAGGGTTAGCAGTCAATCGG+AGG + chr3_4:77961924-77961943 Msa0524510:five_prime_UTR 50.0%
TGGAAGGGCGAGTTAAAGAG+AGG - chr3_4:77962023-77962042 None:intergenic 50.0%
AACTCGCCCTTCCAACATAC+AGG + chr3_4:77962029-77962048 Msa0524510:CDS 50.0%
GACATGGCCTGTATGTTGGA+AGG - chr3_4:77962039-77962058 None:intergenic 50.0%
ATTGCTGACCAGAGTGGTTC+TGG + chr3_4:77962093-77962112 Msa0524510:CDS 50.0%
CTGAGAAGCTAACTCAGCTG+TGG - chr3_4:77962226-77962245 None:intergenic 50.0%
! CACAGCTGAGTTAGCTTCTC+AGG + chr3_4:77962224-77962243 Msa0524510:CDS 50.0%
ATGAGGAAGAGAAAACGGCC+AGG - chr3_4:77962381-77962400 None:intergenic 50.0%
TTGCAGTGCTGTCTTGGATG+AGG + chr3_4:77962583-77962602 Msa0524510:intron 50.0%
TGGCGATGAGGACTTCGAAA+AGG + chr3_4:77962619-77962638 Msa0524510:CDS 50.0%
CAAGCCCAGCCAGCAAATTT+AGG - chr3_4:77962878-77962897 None:intergenic 50.0%
TATGGCAGTGGCAGTACAGT+TGG + chr3_4:77962999-77963018 Msa0524510:intron 50.0%
! TCCGCATTGACAACAGAGAC+AGG - chr3_4:77963052-77963071 None:intergenic 50.0%
ACTCAATAATTGGCGCGGTG+CGG + chr3_4:77963092-77963111 Msa0524510:intron 50.0%
AAAAACAGGACCACCGCACT+AGG - chr3_4:77963386-77963405 None:intergenic 50.0%
!!! TGGGGTATTTTGGTGTGTGC+AGG + chr3_4:77963482-77963501 Msa0524510:intron 50.0%
ACTGGTCACTGAATCCAGCA+TGG - chr3_4:77963508-77963527 None:intergenic 50.0%
TGTGTCATGGCAGCATGGAA+AGG - chr3_4:77963949-77963968 None:intergenic 50.0%
ACTTGTAACAGAGCTTCGCG+GGG + chr3_4:77964176-77964195 Msa0524510:intron 50.0%
!! TTGGAAAGGGTGCTCTCAAC+TGG + chr3_4:77964456-77964475 Msa0524510:three_prime_UTR 50.0%
!! TGGAAAGGGTGCTCTCAACT+GGG + chr3_4:77964457-77964476 Msa0524510:three_prime_UTR 50.0%
AAAATGGCGGTGGTTGCGCA+AGG + chr3_4:77961609-77961628 Msa0524510:five_prime_UTR 55.0%
! GTGGTTGCGCAAGGTGTTTG+TGG + chr3_4:77961618-77961637 Msa0524510:five_prime_UTR 55.0%
CGAAGTCCTCATCGCCAAAG+AGG - chr3_4:77962616-77962635 None:intergenic 55.0%
GTTCTCGCCTTGAAGAGGTC+AGG + chr3_4:77962785-77962804 Msa0524510:intron 55.0%
CTCAATAATTGGCGCGGTGC+GGG + chr3_4:77963093-77963112 Msa0524510:intron 55.0%
! GGGGCACTATTTCGGTAGTG+CGG + chr3_4:77963119-77963138 Msa0524510:intron 55.0%
!! ACTTTTGACACGCGCTGACG+CGG + chr3_4:77963340-77963359 Msa0524510:intron 55.0%
CGGAGTGGAGCGGAAAAAAC+AGG - chr3_4:77963400-77963419 None:intergenic 55.0%
AGCTGCTCTTCTGCAGCTTG+CGG + chr3_4:77963710-77963729 Msa0524510:CDS 55.0%
CGTGTTGGAAGAACAGCCAG+AGG + chr3_4:77964269-77964288 Msa0524510:CDS 55.0%
!! AAGAACAGCCAGAGGTGCCA+GGG + chr3_4:77964277-77964296 Msa0524510:CDS 55.0%
! GCCGAAGGTACATCATGCAG+TGG - chr3_4:77964386-77964405 None:intergenic 55.0%
GTCAATGCGGAAATGCGGCG+CGG + chr3_4:77963061-77963080 Msa0524510:intron 60.0%
TCCGCTCCACTCCGCTATAG+CGG + chr3_4:77963406-77963425 Msa0524510:intron 60.0%
! ACCGCTATAGCGGAGTGGAG+CGG - chr3_4:77963410-77963429 None:intergenic 60.0%
AGCCACGTCGACCGCTATAG+CGG - chr3_4:77963420-77963439 None:intergenic 60.0%
TTGGTGTGTGCAGGCCATGC+TGG + chr3_4:77963491-77963510 Msa0524510:intron 60.0%
!! GAAGAACAGCCAGAGGTGCC+AGG + chr3_4:77964276-77964295 Msa0524510:CDS 60.0%
!! AGAACAGCCAGAGGTGCCAG+GGG + chr3_4:77964278-77964297 Msa0524510:CDS 60.0%
AAATGCCTTGCCCTGTCCCC+TGG - chr3_4:77964297-77964316 None:intergenic 60.0%
TAATTGGCGCGGTGCGGGAG+CGG + chr3_4:77963098-77963117 Msa0524510:intron 65.0%
! GCGGGAGCGGGGCACTATTT+CGG + chr3_4:77963111-77963130 Msa0524510:intron 65.0%
! CGTCGACCGCTATAGCGGAG+TGG - chr3_4:77963415-77963434 None:intergenic 65.0%
CTCCGCTATAGCGGTCGACG+TGG + chr3_4:77963415-77963434 Msa0524510:intron 65.0%
AATTGGCGCGGTGCGGGAGC+GGG + chr3_4:77963099-77963118 Msa0524510:intron 70.0%
ACCACCGCACTAGGCCGAGC+CGG - chr3_4:77963377-77963396 None:intergenic 70.0%
!! AGCCAGAGGTGCCAGGGGAC+AGG + chr3_4:77964283-77964302 Msa0524510:CDS 70.0%
!! GCCAGAGGTGCCAGGGGACA+GGG + chr3_4:77964284-77964303 Msa0524510:CDS 70.0%
AGGTGCCAGGGGACAGGGCA+AGG + chr3_4:77964289-77964308 Msa0524510:CDS 70.0%
ATTGGCGCGGTGCGGGAGCG+GGG + chr3_4:77963100-77963119 Msa0524510:intron 75.0%
TGACGCGGCCTGAGACGCGC+CGG + chr3_4:77963355-77963374 Msa0524510:intron 75.0%
GCCCTGTCCCCTGGCACCTC+TGG - chr3_4:77964288-77964307 None:intergenic 75.0%
!!! CGGCCTGAGACGCGCCGGCT+CGG + chr3_4:77963360-77963379 Msa0524510:intron 80.0%
! AGGCCGAGCCGGCGCGTCTC+AGG - chr3_4:77963366-77963385 None:intergenic 80.0%
!!! CGCGCCGGCTCGGCCTAGTG+CGG + chr3_4:77963370-77963389 Msa0524510:intron 80.0%
!!! GCCGGCTCGGCCTAGTGCGG+TGG + chr3_4:77963373-77963392 Msa0524510:intron 80.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 77961544 77964595 77961544 ID=Msa0524510;Name=Msa0524510
chr3_4 mRNA 77961544 77964595 77961544 ID=Msa0524510-mRNA-1;Parent=Msa0524510;Name=Msa0524510-mRNA-1;_AED=0.08;_eAED=0.08;_QI=456|0.6|0.5|1|0.6|0.66|6|177|403
chr3_4 exon 77961544 77962436 77961544 ID=Msa0524510-mRNA-1:exon:22945;Parent=Msa0524510-mRNA-1
chr3_4 exon 77962589 77962769 77962589 ID=Msa0524510-mRNA-1:exon:22946;Parent=Msa0524510-mRNA-1
chr3_4 exon 77963504 77963525 77963504 ID=Msa0524510-mRNA-1:exon:22947;Parent=Msa0524510-mRNA-1
chr3_4 exon 77963610 77963773 77963610 ID=Msa0524510-mRNA-1:exon:22948;Parent=Msa0524510-mRNA-1
chr3_4 exon 77963874 77964049 77963874 ID=Msa0524510-mRNA-1:exon:22949;Parent=Msa0524510-mRNA-1
chr3_4 exon 77964187 77964595 77964187 ID=Msa0524510-mRNA-1:exon:22950;Parent=Msa0524510-mRNA-1
chr3_4 five_prime_UTR 77961544 77961999 77961544 ID=Msa0524510-mRNA-1:five_prime_utr;Parent=Msa0524510-mRNA-1
chr3_4 CDS 77962000 77962436 77962000 ID=Msa0524510-mRNA-1:cds;Parent=Msa0524510-mRNA-1
chr3_4 CDS 77962589 77962769 77962589 ID=Msa0524510-mRNA-1:cds;Parent=Msa0524510-mRNA-1
chr3_4 CDS 77963504 77963525 77963504 ID=Msa0524510-mRNA-1:cds;Parent=Msa0524510-mRNA-1
chr3_4 CDS 77963610 77963773 77963610 ID=Msa0524510-mRNA-1:cds;Parent=Msa0524510-mRNA-1
chr3_4 CDS 77963874 77964049 77963874 ID=Msa0524510-mRNA-1:cds;Parent=Msa0524510-mRNA-1
chr3_4 CDS 77964187 77964418 77964187 ID=Msa0524510-mRNA-1:cds;Parent=Msa0524510-mRNA-1
chr3_4 three_prime_UTR 77964419 77964595 77964419 ID=Msa0524510-mRNA-1:three_prime_utr;Parent=Msa0524510-mRNA-1
Gene Sequence

>Msa0524510

ATGATTGAATCTCTCCATAACCTCTCTTTAACTCGCCCTTCCAACATACAGGCCATGTCATTTGCTCCTGTTATTGCCGGAAAGTCTTGTATAATTGCTGACCAGAGTGGTTCTGGAAAGACATTAGCTTATCTTGTACCAATCATTCAACGTCTCAGACAACAAGAACTACAAGGACTTCATAAATCTTCTTCACGATCTCCTACTCTTGTTATACTCGCACCCACAGCTGAGTTAGCTTCTCAGGTCTTCCATAATTGTCGTTCAATGTCTAAATCTGGCAAGGTTCCATTCAAATCTATGCTTGTCACTGGTGGCTTCCGACAAAAAACTCAACTCGACACTTTAAACCAGGGTGTTGATGTCTTCATAGCTACACCTGGCCGTTTTCTCTTCCTCATCAACCAAGGCTTTTTGCACTTAACTAATCTAACATGTGCTGTCTTGGATGAGGTTGATATCCTCTTTGGCGATGAGGACTTCGAAAAGGCTCTTCAATGCTTGATTAGTTCTTCACCGGTGGACACACAATATTTATTTGTAACTGCTACTTTACCAAAAAATGTTTACAGCAAACTGGTTAAGTTTTTCCTGATTGTGAAATGGTCATGGGACCTAGCCATGCTGGATTCAGTGACCATGGGTCTGCTGATTATTTTCTTGTTTGTTCAGATAATAGTAGATTGTAGTGGGGAAGATGGACAAGAAAAAACTCTCGACACAGCATTTTTGAACAAGAAAGCTGCTCTTCTGCAGCTTGCGGAGAAGAATCGCGTCCCAAGAACTATTGTGTTCTGCAACAAAATTGAAACATGCAGAAAAGTTGAAAATGCATTAAACCGTTTTGATAGAAAGGGAATTCGCATGCAAGTTCTACCTTTCCATGCTGCCATGACACAAGAATCACGGCTTACTAGTATGAAGGAGTTTACACGTTCTCCACCAAAAGAAGTGTCCCAGTTTATGGTTTGCACGGACAGAGCTTCGCGGGGAATTGACTTCACTAGAGTGGATCATGTAATACTTTTTGACTACCCACGTGATCCAAGTGAATATGTACGGCGTGTTGGAAGAACAGCCAGAGGTGCCAGGGGACAGGGCAAGGCATTTATCTTTGTTGTTGGCAAGCAAGTATCCCTTGCACAAAAAGTAATGGAAAGAAATCGGAAGGGTCATCCACTGCATGATGTACCTTCGGCTTATGAGTATTGA
Protein sequence

>Msa0524510

MIESLHNLSLTRPSNIQAMSFAPVIAGKSCIIADQSGSGKTLAYLVPIIQRLRQQELQGLHKSSSRSPTLVILAPTAELASQVFHNCRSMSKSGKVPFKSMLVTGGFRQKTQLDTLNQGVDVFIATPGRFLFLINQGFLHLTNLTCAVLDEVDILFGDEDFEKALQCLISSSPVDTQYLFVTATLPKNVYSKLVKFFLIVKWSWDLAMLDSVTMGLLIIFLFVQIIVDCSGEDGQEKTLDTAFLNKKAALLQLAEKNRVPRTIVFCNKIETCRKVENALNRFDRKGIRMQVLPFHAAMTQESRLTSMKEFTRSPPKEVSQFMVCTDRASRGIDFTRVDHVILFDYPRDPSEYVRRVGRTARGARGQGKAFIFVVGKQVSLAQKVMERNRKGHPLHDVPSAYEY*