AlfalfaGEDB | Msa1065010

M. sativa cultivar zhongmu-4 / Msa1065010

KEGG: K03120
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006352 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0017025 GO:0018130 GO:0019438 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576
PFAM: PF00352.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1065010	KEH23255.1	94.694	245	1	2	1	236	1	242	1.78e-166	469

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1065010	sp\|P26357\|TBP_SOLTU	95.567	203	6	1	1	203	1	200	1.06e-141	397

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1065010	A0A072UBM6	94.694	245	1	2	1	236	1	242	8.51e-167	469

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007990	Msa1065010	0.806451	8.172720e-50	-8.615850e-47
Msa0032470	Msa1065010	0.807881	4.070438e-50	-8.615850e-47
Msa0042550	Msa1065010	0.819048	1.425194e-52	-8.615850e-47
Msa0045790	Msa1065010	0.810036	1.407046e-50	-8.615850e-47
Msa0046160	Msa1065010	0.806885	6.620425e-50	-8.615850e-47
Msa0047110	Msa1065010	0.800320	1.523081e-48	-8.615850e-47
Msa0054820	Msa1065010	0.837931	3.883155e-57	-8.615850e-47
Msa0056450	Msa1065010	0.820065	8.349179e-53	-8.615850e-47
Msa0058160	Msa1065010	0.824748	6.821350e-54	-8.615850e-47
Msa0058600	Msa1065010	0.803073	4.148818e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1065010	MtrunA17_Chr7g0244531	93.644	236	4	2	1	236	1	225	2.73e-161	445
Msa1065010	MtrunA17_Chr4g0013121	93.923	181	8	1	1	181	1	178	6.42e-121	341
Msa1065010	MtrunA17_Chr3g0110921	67.100	231	62	3	1	231	15	231	4.62e-100	290
Msa1065010	MtrunA17_Chr3g0111031	61.039	154	40	2	26	179	59	192	7.71e-53	169
Msa1065010	MtrunA17_Chr4g0068601	50.000	82	33	3	6	84	1	77	7.77e-16	70.1
Msa1065010	MtrunA17_Chr4g0068591	60.000	50	17	1	36	85	2	48	1.16e-13	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1065010	AT1G55520.2	91.626	203	14	1	1	203	1	200	3.41e-135	378
Msa1065010	AT1G55520.1	91.626	203	14	1	1	203	1	200	3.41e-135	378
Msa1065010	AT1G55520.4	91.626	203	14	1	1	203	1	200	3.41e-135	378
Msa1065010	AT3G13445.1	91.133	203	15	1	1	203	1	200	1.43e-134	377
Msa1065010	AT1G55520.3	92.778	180	10	1	1	180	1	177	1.05e-119	338
Msa1065010	AT3G13445.2	92.222	180	11	1	1	180	1	177	7.05e-119	337

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Find 78 sgRNAs with CRISPR-Local

Find 326 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAACTCACTGAAGAGT+AGG	0.550288	7_2:-58407666	None:intergenic
AAGAACCCTACTTAAAATTA+AGG	0.244771	7_2:-58411739	None:intergenic
AAGAGTGTGTTTGGAGAGAG+CGG	0.453797	7_2:-58407176	None:intergenic
AAGCAGTACCTTAAACTTAG+CGG	0.595117	7_2:-58409982	None:intergenic
AAGTGAGAATTTGTAATAAG+GGG	0.563510	7_2:-58407143	None:intergenic
AAGTTCCCCATACGGCTGGA+GGG	0.615249	7_2:+58410164	Msa1065010:CDS
AATATAAGCAATACTATCTT+GGG	0.443178	7_2:-58410819	None:intergenic
AATGTTGGCTGTTGGATTAG+CGG	0.492412	7_2:+58411281	Msa1065010:CDS
AATTGTTCTGACAGGAGCCA+AGG	0.589296	7_2:+58410854	Msa1065010:CDS
AGAAATTGGAGTTGAAATGA+AGG	0.475515	7_2:-58407212	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGAAGGTAAAAACAGGTT+GGG	-	chr7_2:58407023-58407042	None:intergenic	30.0%
GAAATTGGAGTTGAAATGAA+GGG	-	chr7_2:58407214-58407233	None:intergenic	30.0%
AGAAATTGGAGTTGAAATGA+AGG	-	chr7_2:58407215-58407234	None:intergenic	30.0%
TGTGCTACATAGTAAATTAG+TGG	+	chr7_2:58407902-58407921	Msa1065010:intron	30.0%
ACTCTCAAATATCATACATG+GGG	-	chr7_2:58407963-58407982	None:intergenic	30.0%
GTACTCTCAAATATCATACA+TGG	-	chr7_2:58407965-58407984	None:intergenic	30.0%
TAAAGACACAAGGTAAATGT+TGG	-	chr7_2:58408042-58408061	None:intergenic	30.0%
ATTGACAAATACGGAAAACA+GGG	-	chr7_2:58408139-58408158	None:intergenic	30.0%
AATTGACAAATACGGAAAAC+AGG	-	chr7_2:58408140-58408159	None:intergenic	30.0%
TTTCCGTATTTGTCAATTGT+TGG	+	chr7_2:58408142-58408161	Msa1065010:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	58407009	58411811	58407009	ID=Msa1065010;Name=Msa1065010
chr7_2	mRNA	58407009	58411811	58407009	ID=Msa1065010-mRNA-1;Parent=Msa1065010;Name=Msa1065010-mRNA-1;_AED=0.20;_eAED=0.20;_QI=333\|0.7\|0.81\|1\|0.7\|0.63\|11\|289\|236
chr7_2	exon	58407009	58407306	58407009	ID=Msa1065010-mRNA-1:exon:11067;Parent=Msa1065010-mRNA-1
chr7_2	exon	58407565	58407676	58407565	ID=Msa1065010-mRNA-1:exon:11068;Parent=Msa1065010-mRNA-1
chr7_2	exon	58408511	58408607	58408511	ID=Msa1065010-mRNA-1:exon:11069;Parent=Msa1065010-mRNA-1
chr7_2	exon	58408937	58409008	58408937	ID=Msa1065010-mRNA-1:exon:11070;Parent=Msa1065010-mRNA-1
chr7_2	exon	58409658	58409708	58409658	ID=Msa1065010-mRNA-1:exon:11071;Parent=Msa1065010-mRNA-1
chr7_2	exon	58409951	58409995	58409951	ID=Msa1065010-mRNA-1:exon:11072;Parent=Msa1065010-mRNA-1
chr7_2	exon	58410125	58410217	58410125	ID=Msa1065010-mRNA-1:exon:11073;Parent=Msa1065010-mRNA-1
chr7_2	exon	58410774	58410875	58410774	ID=Msa1065010-mRNA-1:exon:11074;Parent=Msa1065010-mRNA-1
chr7_2	exon	58410964	58411037	58410964	ID=Msa1065010-mRNA-1:exon:11075;Parent=Msa1065010-mRNA-1
chr7_2	exon	58411268	58411302	58411268	ID=Msa1065010-mRNA-1:exon:11076;Parent=Msa1065010-mRNA-1
chr7_2	exon	58411458	58411811	58411458	ID=Msa1065010-mRNA-1:exon:11077;Parent=Msa1065010-mRNA-1
chr7_2	five_prime_UTR	58407009	58407306	58407009	ID=Msa1065010-mRNA-1:five_prime_utr;Parent=Msa1065010-mRNA-1
chr7_2	five_prime_UTR	58407565	58407599	58407565	ID=Msa1065010-mRNA-1:five_prime_utr;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58407600	58407676	58407600	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58408511	58408607	58408511	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58408937	58409008	58408937	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58409658	58409708	58409658	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58409951	58409995	58409951	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58410125	58410217	58410125	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58410774	58410875	58410774	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58410964	58411037	58410964	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58411268	58411302	58411268	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	CDS	58411458	58411522	58411458	ID=Msa1065010-mRNA-1:cds;Parent=Msa1065010-mRNA-1
chr7_2	three_prime_UTR	58411523	58411811	58411523	ID=Msa1065010-mRNA-1:three_prime_utr;Parent=Msa1065010-mRNA-1

Gene Sequence

>Msa1065010

ATGGCTGACCAAGGATTGGAAGGGAGCCAACCTGTGGATCTATCAAAGCATCCTTCTGGAATTGTGCCTACTCTTCAAAATATTGTATCAACTGTCAATTTGGACTGTAAGTTGGAGCTGAAATCAATTGCGCTTCAAGCTCGTAATGCAGAGTACAATCCCAAGGCAAGTTTTCGTTTTGCTGCTGTGATTATGAGGATCCGGGAACCAAAAACAACAGCACTTATTTTTGCATCTGGCAAGATGGTCTGCACTGGAGCTAAGAGTGAGGTGCAATCAAAATTGGCAGCAAGGAAGTATGCTAGAATTATCCAGAAACTGGGTTTCCCCGCTAAGTTTAAGGATTTTAAGATTCAGAACATTGTCGGCTCTTGTGATGTCAAGTTCCCCATACGGCTGGAGGGTCTTGCATATTCCCACGGTGCTTTCTCAAGTTATGAACCAGAGTTGTTTCCAGGACTAATCTATCGAATGAAGCAACCCAAGATAGTATTGCTTATATTCGTATCTGGGAAAATTGTTCTGACAGGAGCCAAGGTGAGAGATGAGACTTACACAGCCTTTGAGAACATATATCCAGTGCTTACTGAGTTCAGGAAAAATCAACAATGCTCAGTCCAATCAAATGTTGGCTGTTGGATTAGCGATTATCGTCATGAGCAAGAGCAAGGTTTTCTGGCAGAGAAGAACGTGGATTGTAGGCTTGATTGA

Protein sequence

>Msa1065010

MADQGLEGSQPVDLSKHPSGIVPTLQNIVSTVNLDCKLELKSIALQARNAEYNPKASFRFAAVIMRIREPKTTALIFASGKMVCTGAKSEVQSKLAARKYARIIQKLGFPAKFKDFKIQNIVGSCDVKFPIRLEGLAYSHGAFSSYEPELFPGLIYRMKQPKIVLLIFVSGKIVLTGAKVRDETYTAFENIYPVLTEFRKNQQCSVQSNVGCWISDYRHEQEQGFLAEKNVDCRLD*