AlfalfaGEDB | Msa0133250

M. sativa cultivar zhongmu-4 / Msa0133250

KEGG: K00521
GO: GO:0000293 GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016722 GO:0016723 GO:0055114
PFAM: PF01794.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133250	sp\|Q9FLW2\|FRO5_ARATH	55.285	246	108	2	258	502	340	584	2.09e-86	284
Msa0133250	sp\|Q9FLW2\|FRO5_ARATH	41.667	252	109	7	14	256	8	230	5.72e-48	180
Msa0133250	sp\|Q9FLW2\|FRO5_ARATH	44.444	117	54	4	416	531	601	707	3.41e-18	91.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133250	A0A2C9VR10	53.673	490	163	5	14	485	2	445	7.42e-166	492
Msa0133250	A0A2C9VR10	78.261	46	10	0	486	531	535	580	4.28e-15	89.7

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0133250	0.809768	1.607099e-50	-8.615850e-47
Msa0005500	Msa0133250	0.808795	2.598153e-50	-8.615850e-47
Msa0011920	Msa0133250	0.807882	4.067928e-50	-8.615850e-47
Msa0021240	Msa0133250	0.801088	1.061903e-48	-8.615850e-47
Msa0024490	Msa0133250	0.804979	1.665841e-49	-8.615850e-47
Msa0025090	Msa0133250	0.807580	4.715455e-50	-8.615850e-47
Msa0030320	Msa0133250	0.822264	2.599133e-53	-8.615850e-47
Msa0033190	Msa0133250	0.803176	3.949665e-49	-8.615850e-47
Msa0038910	Msa0133250	0.944869	8.808481e-104	-8.615850e-47
Msa0046020	Msa0133250	0.804341	2.262971e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133250	MtrunA17_Chr1g0202731	92.070	227	18	0	259	485	346	572	6.64e-147	438
Msa0133250	MtrunA17_Chr1g0202731	79.377	257	32	4	1	257	1	236	6.40e-125	382
Msa0133250	MtrunA17_Chr1g0202731	97.826	46	1	0	486	531	662	707	2.21e-23	104
Msa0133250	MtrunA17_Chr1g0202741	89.474	228	24	0	258	485	340	567	2.15e-143	429
Msa0133250	MtrunA17_Chr1g0202741	64.173	254	68	5	4	257	1	231	3.44e-91	293
Msa0133250	MtrunA17_Chr1g0202741	86.957	46	6	0	486	531	657	702	1.49e-20	95.5
Msa0133250	MtrunA17_Chr8g0348531	57.940	233	96	2	258	489	354	585	7.99e-78	258
Msa0133250	MtrunA17_Chr8g0348531	41.202	233	105	5	28	254	36	242	7.22e-44	165
Msa0133250	MtrunA17_Chr8g0348531	63.043	46	17	0	486	531	671	716	1.37e-12	70.5
Msa0133250	MtrunA17_Chr8g0348521	58.120	234	93	4	258	489	341	571	1.19e-77	258

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133250	AT5G23990.3	55.285	246	108	2	258	502	200	444	1.14e-88	284
Msa0133250	AT5G23990.3	60.714	84	33	0	174	257	8	91	1.21e-26	114
Msa0133250	AT5G23990.3	44.444	117	54	4	416	531	461	567	2.98e-19	91.7
Msa0133250	AT5G23990.2	55.285	246	108	2	258	502	332	576	1.64e-87	284
Msa0133250	AT5G23990.2	43.033	244	109	6	14	256	8	222	5.45e-51	186
Msa0133250	AT5G23990.2	44.444	117	54	4	416	531	593	699	3.33e-19	91.7
Msa0133250	AT5G23990.1	55.285	246	108	2	258	502	290	534	1.92e-87	283
Msa0133250	AT5G23990.1	63.636	77	28	0	181	257	105	181	6.29e-25	109
Msa0133250	AT5G23990.1	44.444	117	54	4	416	531	551	657	4.70e-19	91.3
Msa0133250	AT5G23980.1	53.659	246	112	2	258	502	332	576	5.41e-85	278

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Find 137 sgRNAs with CRISPR-Local

Find 351 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGTGAAGACTTCTGT+TGG	0.548873	1_3:-64511593	None:intergenic
AAAAGGGTGCCATTGCAGCT+TGG	0.569692	1_3:-64514888	None:intergenic
AAACGTGCAGCATATTGGAT+AGG	0.524943	1_3:+64511442	Msa0133250:five_prime_UTR
AACGTGCAGCATATTGGATA+GGG	0.567635	1_3:+64511443	Msa0133250:five_prime_UTR
AACTAGAAGTCACTGTAAAA+GGG	0.514748	1_3:-64514904	None:intergenic
AAGACACCACTATGTATATA+TGG	0.382103	1_3:-64511927	None:intergenic
AAGACTTCTGTTGGTTACAA+TGG	0.530051	1_3:-64511584	None:intergenic
AATTGGAATTGTTTCAGCTA+TGG	0.474439	1_3:+64512599	Msa0133250:CDS
ACAAAATTAAGTGAAACTTT+TGG	0.346777	1_3:-64517357	None:intergenic
ACAAATATTACTATTATTAT+TGG	0.185605	1_3:-64511883	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTACTGTGCTTAATATGTAG+TGG	-	chr1_3:64511413-64511432	None:intergenic	30.0%
ATGGAATATAAGATATGTGG+AGG	-	chr1_3:64511624-64511643	None:intergenic	30.0%
ATTGTGATCCTAAGCAATAA+TGG	-	chr1_3:64511643-64511662	None:intergenic	30.0%
CAATCACCATATATACATAG+TGG	+	chr1_3:64511921-64511940	Msa0133250:five_prime_UTR	30.0%
AAGACACCACTATGTATATA+TGG	-	chr1_3:64511930-64511949	None:intergenic	30.0%
CTAAGATCTACAAAAAGATC+TGG	+	chr1_3:64512131-64512150	Msa0133250:CDS	30.0%
TTCAATTCTACATACTTCAG+AGG	+	chr1_3:64512175-64512194	Msa0133250:CDS	30.0%
TCAATTCTACATACTTCAGA+GGG	+	chr1_3:64512176-64512195	Msa0133250:CDS	30.0%
ATAGCTGAAACAATTCCAAT+TGG	-	chr1_3:64512600-64512619	None:intergenic	30.0%
AATTGGAATTGTTTCAGCTA+TGG	+	chr1_3:64512599-64512618	Msa0133250:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	64511388	64517365	64511388	ID=Msa0133250;Name=Msa0133250
chr1_3	mRNA	64511388	64517365	64511388	ID=Msa0133250-mRNA-1;Parent=Msa0133250;Name=Msa0133250-mRNA-1;_AED=0.28;_eAED=0.29;_QI=643\|0.33\|0.42\|1\|0.83\|0.85\|7\|271\|531
chr1_3	exon	64511388	64512202	64511388	ID=Msa0133250-mRNA-1:exon:21729;Parent=Msa0133250-mRNA-1
chr1_3	exon	64512286	64512413	64512286	ID=Msa0133250-mRNA-1:exon:21730;Parent=Msa0133250-mRNA-1
chr1_3	exon	64512495	64512727	64512495	ID=Msa0133250-mRNA-1:exon:21731;Parent=Msa0133250-mRNA-1
chr1_3	exon	64512858	64513096	64512858	ID=Msa0133250-mRNA-1:exon:21732;Parent=Msa0133250-mRNA-1
chr1_3	exon	64514837	64515077	64514837	ID=Msa0133250-mRNA-1:exon:21733;Parent=Msa0133250-mRNA-1
chr1_3	exon	64516094	64516536	64516094	ID=Msa0133250-mRNA-1:exon:21734;Parent=Msa0133250-mRNA-1
chr1_3	exon	64516955	64517365	64516955	ID=Msa0133250-mRNA-1:exon:21735;Parent=Msa0133250-mRNA-1
chr1_3	five_prime_UTR	64511388	64512030	64511388	ID=Msa0133250-mRNA-1:five_prime_utr;Parent=Msa0133250-mRNA-1
chr1_3	CDS	64512031	64512202	64512031	ID=Msa0133250-mRNA-1:cds;Parent=Msa0133250-mRNA-1
chr1_3	CDS	64512286	64512413	64512286	ID=Msa0133250-mRNA-1:cds;Parent=Msa0133250-mRNA-1
chr1_3	CDS	64512495	64512727	64512495	ID=Msa0133250-mRNA-1:cds;Parent=Msa0133250-mRNA-1
chr1_3	CDS	64512858	64513096	64512858	ID=Msa0133250-mRNA-1:cds;Parent=Msa0133250-mRNA-1
chr1_3	CDS	64514837	64515077	64514837	ID=Msa0133250-mRNA-1:cds;Parent=Msa0133250-mRNA-1
chr1_3	CDS	64516094	64516536	64516094	ID=Msa0133250-mRNA-1:cds;Parent=Msa0133250-mRNA-1
chr1_3	CDS	64516955	64517094	64516955	ID=Msa0133250-mRNA-1:cds;Parent=Msa0133250-mRNA-1
chr1_3	three_prime_UTR	64517095	64517365	64517095	ID=Msa0133250-mRNA-1:three_prime_utr;Parent=Msa0133250-mRNA-1

Gene Sequence

>Msa0133250

ATGTGCATTATGATGATGAAGAGACACACAATAATATTCCTAAGAATCATTTTTCTTGTGATGTTTCTTGGGTGGCTATCTGTTTGGATCTTGTTGCCTACTAAGATCTACAAAAAGATCTGGGCTCCCATGCTACAAAACAAGTTCAATTCTACATACTTCAGAGGGCAAGTAGTTCTTAACTTTTTTAGGGATAAATCTCCTGCTTTTCACATTCCCTATGATGTTCATTGGAGCTCTGAGTTGTATTTATCTCCATTTTCATCAAGAAAAAATAACAGAAAAGTTGCCTTCCAGAAGTTTCCCTTTAGAAGTCATAGATATAGTATATGTTTTGGTTCCAGCAGTGGCGCTCTAAAGAGATGTTTAGGCTTCCTTAGACGTCCATTCCTGGTTATGTCTCCAATTGGAATTGTTTCAGCTATGGAGCTTATTTTTGTACTCATGTTTGCTGCACTCTTGATATGGTCCCTTTCCAATTACTTACACACCGGTTTCGATCACCTCCGCATGCACAAACAAGATGAGAAAATATGGGAAGCAAAGTTGAGGAGTGTATCTTTGAGACTTGCTTACATAGGAAATATATGTTTGGCATTTTTGTTCTTTCCGGTCACAAGAATGTCTTCTATTCTGCCTCTTGTTGGACTCACATCTGAGTCAAGCATCAAGTATCATATCTGGCTTGGACATTTATCTATGGTTCTTTTTTCTGCTCATTCCATTGGTTTTATCATTTATTGGGCCATCACCAACCAAATGATTGAGGTAGGTTTATACTATAACCCCACAAGCTTGGTGTTCATAAATGTTCCAAAGGTTTCCAAGCTGCAATGGCACCCTTTTACAGTGACTTCTAGTTGTAATATGGAGACTAACTATCTGAGTATTGCCATAAAAAATGTGGGCAGCTGGTCAAACAAGCTGTATCAGGAACTTTCTTCTTCGACTTTGGATCATCTTAATATTTCAGTTGAAGGACCCTACGGACCCCATACTGCACAATTTCTGAGGCATGAGCAGATTGTGATGGTGAGTGGCGGAAGTGGCGTAACTCCCTTCATATCTATAATCCGAGATCTCATATTTCAAAGCCAACAACAAGAATTCCAACCTCCAAAACTCATACTTGTTTGTATTTTCAAAAGCTATGCTGACCTAACCATGTTAGATCTTATGCTTCCTGTTTCTGGCTCAACAGCTCAAATCTCACAGCTTCAGTTACAAATTGAAGCTTACATCACAAGAGAAAAAGAACAACCTTCAAGAGATGCTAATAAACAAATCAAAACTATATGGTTTAAAACAAACCTCTCAGATTCTCCTATCTCAGGTGTATTAGGCCCAAATAACTGGTTATGGCTTGGTGCTATAATCACATCATCGTTCATCATGTTTCTCCTCTTTTTAGGAATTGTTACTCGTTACTATATTTATCCAATTGAAAATAACTCAGAAATTCTGTTTGAGTGTGAAGGCAAAGATGTTGGAGTTATGACTTGTGGTCCAAGAAAAATGAGACATGAAGTTGCTAGAATTTGTGCTTCTGGTTTGGCTGATAATCTTCACTTTGAATCAATCAGCTTTAATTGGTGA

Protein sequence

>Msa0133250

MCIMMMKRHTIIFLRIIFLVMFLGWLSVWILLPTKIYKKIWAPMLQNKFNSTYFRGQVVLNFFRDKSPAFHIPYDVHWSSELYLSPFSSRKNNRKVAFQKFPFRSHRYSICFGSSSGALKRCLGFLRRPFLVMSPIGIVSAMELIFVLMFAALLIWSLSNYLHTGFDHLRMHKQDEKIWEAKLRSVSLRLAYIGNICLAFLFFPVTRMSSILPLVGLTSESSIKYHIWLGHLSMVLFSAHSIGFIIYWAITNQMIEVGLYYNPTSLVFINVPKVSKLQWHPFTVTSSCNMETNYLSIAIKNVGSWSNKLYQELSSSTLDHLNISVEGPYGPHTAQFLRHEQIVMVSGGSGVTPFISIIRDLIFQSQQQEFQPPKLILVCIFKSYADLTMLDLMLPVSGSTAQISQLQLQIEAYITREKEQPSRDANKQIKTIWFKTNLSDSPISGVLGPNNWLWLGAIITSSFIMFLLFLGIVTRYYIYPIENNSEILFECEGKDVGVMTCGPRKMRHEVARICASGLADNLHFESISFNW*