AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0567530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567530 MtrunA17_Chr8g0382231 97.192 641 7 1 1 641 1 630 0.0 1296
Msa0567530 MtrunA17_Chr4g0050021 41.679 691 290 13 26 641 17 669 1.12e-174 512
Msa0567530 MtrunA17_Chr2g0279481 41.453 702 297 16 13 634 3 670 1.05e-172 508
Msa0567530 MtrunA17_Chr5g0401451 34.966 592 302 12 19 577 10 551 3.99e-114 355
Msa0567530 MtrunA17_Chr1g0191711 34.392 567 312 11 24 581 29 544 1.59e-112 348
Msa0567530 MtrunA17_Chr2g0303541 32.701 633 340 11 37 618 10 607 6.78e-112 350
Msa0567530 MtrunA17_Chr1g0181181 34.535 527 282 11 41 544 15 501 5.27e-102 322
Msa0567530 MtrunA17_Chr2g0285511 32.855 627 321 16 52 633 26 597 1.12e-101 322
Msa0567530 MtrunA17_Chr4g0010091 33.582 536 287 8 20 527 2 496 2.37e-101 321
Msa0567530 MtrunA17_Chr1g0195911 33.733 584 287 13 52 582 36 572 1.03e-100 320
Msa0567530 MtrunA17_Chr4g0053841 35.252 556 274 12 52 570 13 519 1.86e-100 318
Msa0567530 MtrunA17_Chr2g0320691 30.116 601 334 11 44 591 15 582 5.01e-100 318
Msa0567530 MtrunA17_Chr8g0374011 34.735 547 253 11 47 529 15 521 5.70e-99 317
Msa0567530 MtrunA17_Chr5g0401341 32.258 589 282 15 38 563 11 545 9.44e-99 315
Msa0567530 MtrunA17_Chr8g0367891 36.810 489 255 8 55 522 29 484 2.79e-98 313
Msa0567530 MtrunA17_Chr7g0268781 33.333 621 303 16 52 613 36 604 3.49e-98 313
Msa0567530 MtrunA17_Chr1g0150191 32.545 550 269 12 46 546 21 517 9.83e-98 312
Msa0567530 MtrunA17_Chr1g0207461 32.877 584 275 18 42 563 18 546 9.02e-96 307
Msa0567530 MtrunA17_Chr7g0245201 35.225 511 267 9 42 527 51 522 1.75e-94 304
Msa0567530 MtrunA17_Chr5g0412591 32.911 553 254 10 42 539 16 506 5.86e-90 291
Msa0567530 MtrunA17_Chr4g0076521 33.147 537 300 11 45 551 19 526 7.39e-89 288
Msa0567530 MtrunA17_Chr3g0108331 31.177 603 344 14 52 641 24 568 1.13e-88 286
Msa0567530 MtrunA17_Chr1g0199631 30.808 594 306 13 70 611 8 548 1.16e-88 287
Msa0567530 MtrunA17_Chr5g0432861 31.827 531 314 8 52 572 25 517 2.00e-84 276
Msa0567530 MtrunA17_Chr2g0290741 31.197 593 328 14 35 598 8 549 4.21e-82 270
Msa0567530 MtrunA17_Chr4g0003891 35.897 312 154 6 215 523 46 314 1.38e-62 214
Msa0567530 MtrunA17_Chr2g0332281 36.452 310 157 6 215 523 24 294 1.12e-59 206
Msa0567530 MtrunA17_Chr4g0037661 26.128 532 274 13 95 570 49 517 1.90e-46 172
Msa0567530 MtrunA17_Chr1g0163311 24.505 555 294 17 55 541 6 503 9.39e-42 159
Msa0567530 MtrunA17_Chr4g0023801 23.583 547 316 13 97 597 50 540 6.02e-38 148
Msa0567530 MtrunA17_Chr2g0280541 23.210 461 268 14 119 553 1 401 9.23e-27 114
Msa0567530 MtrunA17_Chr1g0206621 37.324 142 78 2 59 194 75 211 4.58e-26 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567530 AT5G10250.2 57.853 624 239 8 22 641 19 622 0.0 697
Msa0567530 AT5G10250.1 56.731 624 231 8 22 641 19 607 0.0 677
Msa0567530 AT5G64330.1 37.551 735 278 14 26 617 24 720 5.56e-157 470
Msa0567530 AT5G64330.2 40.630 603 203 9 44 527 44 610 2.34e-149 447
Msa0567530 AT5G64330.3 40.830 578 187 9 69 527 10 551 4.66e-142 427
Msa0567530 AT5G67385.1 36.640 494 252 8 52 525 25 477 5.82e-108 338
Msa0567530 AT5G48800.1 33.969 524 259 6 34 527 23 489 4.52e-100 318
Msa0567530 AT1G03010.2 34.142 618 295 15 52 606 9 577 6.60e-100 318
Msa0567530 AT5G13600.1 32.492 634 317 18 44 626 18 591 7.80e-100 317
Msa0567530 AT5G67385.4 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567530 AT5G67385.5 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567530 AT5G67385.2 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567530 AT5G67385.3 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567530 AT5G03250.1 33.223 605 308 15 54 610 28 584 1.10e-99 317
Msa0567530 AT1G03010.1 34.237 590 286 12 52 582 36 582 1.36e-99 318
Msa0567530 AT1G03010.3 33.713 614 303 13 52 606 35 603 1.66e-99 317
Msa0567530 AT1G30440.1 33.397 527 271 8 47 527 21 513 2.63e-99 318
Msa0567530 AT2G47860.1 32.823 588 286 11 52 582 37 572 6.26e-97 311
Msa0567530 AT5G67440.3 35.108 507 248 11 46 522 21 476 9.10e-97 308
Msa0567530 AT5G67440.1 35.108 507 248 11 46 522 21 476 9.10e-97 308
Msa0567530 AT3G44820.1 31.376 596 319 13 47 582 18 583 4.74e-95 306
Msa0567530 AT3G49970.1 36.219 439 232 9 60 496 2 394 1.82e-93 298
Msa0567530 AT3G49970.2 36.219 439 232 9 60 496 2 394 1.82e-93 298
Msa0567530 AT1G67900.5 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567530 AT1G67900.1 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567530 AT1G67900.3 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567530 AT1G67900.6 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567530 AT1G67900.2 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567530 AT1G67900.4 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567530 AT2G47860.3 32.455 607 286 13 52 582 37 595 2.71e-93 302
Msa0567530 AT2G14820.2 32.902 541 264 12 38 532 11 498 6.00e-93 300
Msa0567530 AT2G14820.1 32.902 541 264 12 38 532 11 498 6.00e-93 300
Msa0567530 AT3G08570.1 32.743 565 290 12 52 572 34 552 2.24e-92 298
Msa0567530 AT5G67440.2 34.908 487 236 11 66 522 4 439 2.92e-90 290
Msa0567530 AT3G50840.1 30.693 606 348 13 47 630 11 566 1.44e-89 290
Msa0567530 AT2G30520.1 33.673 490 274 9 52 531 30 478 7.32e-89 288
Msa0567530 AT3G08660.1 30.795 591 321 13 52 616 34 562 7.77e-89 288
Msa0567530 AT4G37590.1 31.579 570 281 15 38 563 11 515 9.35e-88 285
Msa0567530 AT5G66560.1 32.212 565 256 12 47 527 14 535 2.96e-87 286
Msa0567530 AT3G26490.1 32.486 551 249 11 43 526 17 511 7.85e-86 280
Msa0567530 AT3G26490.3 32.486 551 249 11 43 526 17 511 7.85e-86 280
Msa0567530 AT3G26490.4 32.486 551 249 11 43 526 17 511 7.85e-86 280
Msa0567530 AT4G31820.1 32.727 495 245 12 46 505 21 462 7.50e-85 277
Msa0567530 AT3G08660.3 30.991 555 297 11 88 616 8 502 1.86e-84 275
Msa0567530 AT5G66560.4 34.810 474 225 10 95 527 66 496 1.89e-84 278
Msa0567530 AT5G66560.3 34.810 474 225 10 95 527 76 506 2.19e-84 278
Msa0567530 AT5G47800.3 32.305 486 274 10 43 505 17 470 3.92e-83 272
Msa0567530 AT5G47800.1 32.305 486 274 10 43 505 17 470 3.92e-83 272
Msa0567530 AT5G47800.4 32.305 486 274 10 43 505 17 470 3.92e-83 272
Msa0567530 AT3G50840.2 31.004 558 313 13 95 630 23 530 8.44e-82 268
Msa0567530 AT3G50840.3 31.004 558 313 13 95 630 23 530 8.44e-82 268
Msa0567530 AT5G47800.2 32.305 486 274 10 43 505 130 583 1.13e-81 271
Msa0567530 AT2G30520.2 33.404 470 262 9 72 531 2 430 2.68e-81 267
Msa0567530 AT2G30520.3 33.404 470 262 9 72 531 2 430 2.68e-81 267
Msa0567530 AT4G31820.2 33.710 442 206 11 99 505 20 409 9.78e-78 257
Msa0567530 AT2G23050.1 30.769 507 262 10 41 526 16 454 1.51e-77 255
Msa0567530 AT2G47860.4 33.705 448 219 8 52 461 37 444 1.09e-74 246
Msa0567530 AT2G47860.6 33.191 467 219 10 52 461 37 467 1.47e-71 239
Msa0567530 AT5G03250.2 31.538 520 261 14 138 610 1 472 2.48e-71 239
Msa0567530 AT2G47860.5 31.474 502 239 10 138 582 1 454 2.10e-68 232
Msa0567530 AT2G47860.2 31.474 502 239 10 138 582 1 454 2.10e-68 232
Msa0567530 AT3G26490.2 31.481 486 221 10 43 461 17 457 2.46e-68 231
Msa0567530 AT3G08660.2 31.494 435 235 8 52 468 34 423 2.17e-67 227
Msa0567530 AT1G52770.1 32.518 409 214 9 197 583 10 378 7.44e-67 226
Msa0567530 AT5G66560.2 30.261 499 232 11 47 461 14 480 3.50e-64 219
Msa0567530 AT3G15570.1 35.000 320 158 7 215 523 29 309 6.31e-58 202
Msa0567530 AT3G19850.1 29.103 457 237 9 94 508 51 462 8.54e-56 199
Msa0567530 AT2G23050.2 29.557 406 201 9 138 526 1 338 8.48e-53 186
Msa0567530 AT5G48130.1 27.083 480 269 10 52 505 39 463 2.36e-52 191
Msa0567530 AT1G50280.2 26.966 445 256 8 95 505 18 427 7.94e-44 164
Msa0567530 AT1G50280.1 26.966 445 256 8 95 505 51 460 8.72e-44 165
Msa0567530 AT3G03510.1 26.840 462 258 14 107 560 8 397 5.13e-37 144
Msa0567530 AT5G17580.2 25.714 490 290 12 42 505 10 451 1.79e-36 144
Msa0567530 AT5G17580.1 26.950 423 245 10 105 505 56 436 9.69e-36 142
Msa0567530 AT3G22104.1 25.664 452 248 12 76 496 26 420 8.92e-35 139
Msa0567530 AT3G22104.4 24.561 399 217 9 125 496 4 345 4.40e-27 115
Msa0567530 AT3G22104.2 24.561 399 217 9 125 496 4 345 4.40e-27 115
Msa0567530 AT3G22104.3 24.561 399 217 9 125 496 4 345 4.40e-27 115

Find 139 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTGTCAGACTGACTTTC+AGG 0.132980 4_1:-68845261 None:intergenic
CACAGCATCTTCTAACAATT+TGG 0.179939 4_1:-68846540 None:intergenic
CTCCAGCCTGATAGATTCTT+TGG 0.216318 4_1:-68848860 None:intergenic
GAGGAGGAATTATGCTTATT+AGG 0.225056 4_1:-68846894 None:intergenic
ATTCAATATTAGTAGGCTTT+TGG 0.241298 4_1:+68847385 Msa0567530:CDS
CATTTGTCGTGCTTTCTTCA+TGG 0.250887 4_1:+68846458 Msa0567530:CDS
CAGCCTCTAATCTCAAATTC+TGG 0.250919 4_1:+68846244 Msa0567530:CDS
TGCGGATAATTTCAGCAATT+AGG 0.253941 4_1:+68846674 Msa0567530:CDS
AACATTGTAATTGAAAGATT+TGG 0.268935 4_1:-68847437 None:intergenic
TTCCCAGAATTTGAGATTAG+AGG 0.277466 4_1:-68846247 None:intergenic
AAGCTGGTGGTTCGATGATT+TGG 0.289085 4_1:+68846627 Msa0567530:CDS
GACTTTCAGGGTCTTGTGCT+TGG 0.289224 4_1:-68845248 None:intergenic
GCGGATAATTTCAGCAATTA+GGG 0.291903 4_1:+68846675 Msa0567530:CDS
TTAGAAGGGCTTAGAGGATA+TGG 0.292438 4_1:+68846778 Msa0567530:CDS
CTCAAGCTTGAAAACTTTCC+AGG 0.304064 4_1:+68846274 Msa0567530:CDS
TATCAGGCTGGAGTTTAAAT+TGG 0.321879 4_1:+68848870 Msa0567530:CDS
GCACATTATAAGTGGTTTCC+TGG 0.326824 4_1:-68845564 None:intergenic
TGTCTATAGTTTCAGATGTC+TGG 0.334509 4_1:-68848727 None:intergenic
ACCTGGACAACTGTTCTTAG+AGG 0.338185 4_1:-68847754 None:intergenic
TGCGTTGTATACTTTGGTTC+AGG 0.340379 4_1:-68848614 None:intergenic
ACTTTCAGGGTCTTGTGCTT+GGG 0.351122 4_1:-68845247 None:intergenic
CCTACTAATATTGAATTTCC+CGG 0.364748 4_1:-68847378 None:intergenic
GAGGAGTTAGAAGGGCTTAG+AGG 0.370662 4_1:+68846772 Msa0567530:CDS
ACAATTCTAAAATTCTGTTA+TGG 0.376842 4_1:+68846316 Msa0567530:CDS
TGCAGAGTCTTCTACGGTCT+TGG 0.377991 4_1:-68847666 None:intergenic
TTCTACGGTCTTGGTCAGTT+AGG 0.381517 4_1:-68847657 None:intergenic
CCGGGAAATTCAATATTAGT+AGG 0.395757 4_1:+68847378 Msa0567530:CDS
TTTACCAATGATCTCTGGTT+TGG 0.398294 4_1:-68846707 None:intergenic
GATGCTGCATCCTCACTTGC+GGG 0.402710 4_1:-68846595 None:intergenic
GACAAACCAAAGAATCTATC+AGG 0.413587 4_1:+68848854 Msa0567530:CDS
ACACGGAGAGCCTTCTTCTC+GGG 0.418325 4_1:-68848994 None:intergenic
GAGCTAATTGCTGCAAGCTT+GGG 0.418641 4_1:-68845195 None:intergenic
TCTAACTCCTCATACATTCC+TGG 0.422868 4_1:-68846760 None:intergenic
GACCTTTAGGTAGGTGTCAA+TGG 0.427446 4_1:-68847522 None:intergenic
TTAATTCCTAGATATCAAAA+TGG 0.429080 4_1:+68847054 Msa0567530:CDS
AGCCTCTAATCTCAAATTCT+GGG 0.431924 4_1:+68846245 Msa0567530:CDS
ATGCAACTGCAACAAAAGTC+CGG 0.435207 4_1:+68847359 Msa0567530:CDS
TCTGGTTTCATATATCAGTT+AGG 0.435439 4_1:+68845146 Msa0567530:five_prime_UTR
GACACGGAGAGCCTTCTTCT+CGG 0.443418 4_1:-68848995 None:intergenic
TTATCCGAGTTGAAGTCTAT+AGG 0.445995 4_1:-68846343 None:intergenic
TATCCGAGTTGAAGTCTATA+GGG 0.446756 4_1:-68846342 None:intergenic
AGAACCTGCGTTGTATACTT+TGG 0.448430 4_1:-68848620 None:intergenic
CTTGAATTGAAAAGTCTGTT+GGG 0.449484 4_1:-68845540 None:intergenic
TTCTGTCAGACTGACTTTCA+GGG 0.449988 4_1:-68845260 None:intergenic
TCTACGGTCTTGGTCAGTTA+GGG 0.449995 4_1:-68847656 None:intergenic
ATTAATTTAGACAGCGTGTC+TGG 0.450562 4_1:+68845128 Msa0567530:five_prime_UTR
TCCTTCCATGTAAAACCAAC+AGG 0.451380 4_1:+68848908 Msa0567530:CDS
ATACATTTACCAATGATCTC+TGG 0.452219 4_1:-68846712 None:intergenic
AAGTGGAATCAACTCTCAAC+AGG 0.453660 4_1:+68848697 Msa0567530:CDS
TGAGCTAATTGCTGCAAGCT+TGG 0.454672 4_1:-68845196 None:intergenic
TCTCTAGTTTCACCTCCTGT+TGG 0.456759 4_1:-68848923 None:intergenic
TACCTTGTGCACATTATAAG+TGG 0.465167 4_1:-68845572 None:intergenic
GGAATGTATGAGGAGTTAGA+AGG 0.465676 4_1:+68846763 Msa0567530:CDS
CTTGGTCAGTTAGGGAAGGA+TGG 0.466551 4_1:-68847648 None:intergenic
AGCTATTGTGACATGTTTGT+TGG 0.475232 4_1:-68845316 None:intergenic
TGAAATGATCGAGTATGTTC+TGG 0.476508 4_1:-68847482 None:intergenic
ACTTGAATTGAAAAGTCTGT+TGG 0.476835 4_1:-68845541 None:intergenic
ATTTCAGCAATTAGGGCAAA+GGG 0.478066 4_1:+68846682 Msa0567530:CDS
ATATCAAAATGCGGCTACAT+AGG 0.478687 4_1:+68846208 Msa0567530:CDS
ATCTGCAACAAGAACTTACA+TGG 0.493501 4_1:-68846931 None:intergenic
GCCTCTAAGAACAGTTGTCC+AGG 0.494899 4_1:+68847753 Msa0567530:CDS
GCTATTGTGACATGTTTGTT+GGG 0.495372 4_1:-68845315 None:intergenic
ACGAGTGTGGTTTGGTGATG+TGG 0.497567 4_1:-68848960 None:intergenic
GATGCTGTGATTCTATAGCT+TGG 0.503818 4_1:+68846554 Msa0567530:CDS
TGTGAGGAAAGCTTCAGTCT+TGG 0.507277 4_1:-68846437 None:intergenic
GGAACCAAACCAGAGATCAT+TGG 0.508422 4_1:+68846703 Msa0567530:CDS
GATCTACGACGAGTGTGGTT+TGG 0.513023 4_1:-68848968 None:intergenic
CTTGTTGCAGATGCTAAAGA+TGG 0.513993 4_1:+68846942 Msa0567530:CDS
GAAAAGTCTGTTGGGATTCG+AGG 0.514401 4_1:-68845532 None:intergenic
AGCAATGCAAGAGAAGGAAC+CGG 0.515645 4_1:+68848670 Msa0567530:CDS
CATTCCTGGTAGCCATCTCT+TGG 0.516344 4_1:-68846746 None:intergenic
CTGTGATTCTATAGCTTGGA+AGG 0.517789 4_1:+68846558 Msa0567530:CDS
TCAGACCTTGAAAAGAGAGT+TGG 0.522254 4_1:+68846997 Msa0567530:CDS
GTGTAGAATTGTAGTTAAGA+TGG 0.523176 4_1:-68845083 None:intergenic
CAGGCTGGAGTTTAAATTGG+CGG 0.523456 4_1:+68848873 Msa0567530:CDS
TAGAAGGGCTTAGAGGATAT+GGG 0.526409 4_1:+68846779 Msa0567530:CDS
GTAGATCAGCCCCGAGAAGA+AGG 0.527618 4_1:+68848984 Msa0567530:CDS
CTTGTGAAAATCTATCTCCA+TGG 0.528028 4_1:+68846506 Msa0567530:CDS
CCTTGAAAAGAGAGTTGGTA+TGG 0.528846 4_1:+68847002 Msa0567530:CDS
TAGACATAGCAAGGAGCAAA+CGG 0.529211 4_1:+68846858 Msa0567530:CDS
AACCAAAGAATCTATCAGGC+TGG 0.531250 4_1:+68848858 Msa0567530:CDS
AGATATCAAAATGGAGGTCA+AGG 0.533332 4_1:+68847063 Msa0567530:CDS
TGCAACTGCAACAAAAGTCC+GGG 0.536493 4_1:+68847360 Msa0567530:CDS
TTGGTCAGTTAGGGAAGGAT+GGG 0.536654 4_1:-68847647 None:intergenic
AATTTCAGCAATTAGGGCAA+AGG 0.537477 4_1:+68846681 Msa0567530:CDS
AAAAGTCTGTTGGGATTCGA+GGG 0.537749 4_1:-68845531 None:intergenic
AAACATGAAATCACTTCAAG+TGG 0.548709 4_1:+68845224 Msa0567530:exon
AGATGCTGCATCCTCACTTG+CGG 0.555801 4_1:-68846596 None:intergenic
TTGTGAAAATCTATCTCCAT+GGG 0.559597 4_1:+68846507 Msa0567530:CDS
TAAAAGGCTGACCTTTAGGT+AGG 0.562453 4_1:-68847531 None:intergenic
GAGAGCAGCAATGCAAGAGA+AGG 0.563979 4_1:+68848664 Msa0567530:CDS
TATGCCAAGAGATGGCTACC+AGG 0.565928 4_1:+68846742 Msa0567530:CDS
GCAGTAATTCAGCTAAGACT+TGG 0.571771 4_1:-68847460 None:intergenic
ATGACTGAAGAACTAGAAGA+TGG 0.572238 4_1:+68846403 Msa0567530:CDS
GATCTGTTGCAACTTCAGCT+AGG 0.573474 4_1:-68847415 None:intergenic
TAGAATTGTAGTTAAGATGG+TGG 0.574829 4_1:-68845080 None:intergenic
CAGCATCTAATCAAGAAAGC+TGG 0.575400 4_1:+68846611 Msa0567530:CDS
GTTCTGCAGTTCTACCATGT+TGG 0.579053 4_1:-68848786 None:intergenic
GAGTTGATTCCACTTTGTGC+CGG 0.581941 4_1:-68848689 None:intergenic
CACGGAGAGCCTTCTTCTCG+GGG 0.584375 4_1:-68848993 None:intergenic
ATGGCTACCAGGAATGTATG+AGG 0.585069 4_1:+68846753 Msa0567530:CDS
TCAAATGTTTCACATCCACC+TGG 0.585855 4_1:-68846292 None:intergenic
AGCCATTGACACCTACCTAA+AGG 0.587167 4_1:+68847520 Msa0567530:CDS
TCATGCAATATGCCAAGAGA+TGG 0.594644 4_1:+68846734 Msa0567530:CDS
CATGGGACAGCATCTTGTTG+AGG 0.601532 4_1:-68846913 None:intergenic
AGAGAGCACTAGACATAGCA+AGG 0.603854 4_1:+68846849 Msa0567530:CDS
CGAGTGTGGTTTGGTGATGT+GGG 0.605082 4_1:-68848959 None:intergenic
GGGCTTAGAGGATATGGGCA+TGG 0.605115 4_1:+68846784 Msa0567530:CDS
GAATGTATGAGGAGTTAGAA+GGG 0.605950 4_1:+68846764 Msa0567530:CDS
CGGTCTTGGTCAGTTAGGGA+AGG 0.612259 4_1:-68847652 None:intergenic
CGAGAATGTAAAGTCCAACA+TGG 0.615865 4_1:+68848772 Msa0567530:CDS
CATAAAATGATCAATGCGGA+GGG 0.617020 4_1:-68846653 None:intergenic
GGGACAGCATCTTGTTGAGG+AGG 0.618679 4_1:-68846910 None:intergenic
GAGAAGGAACCGGCACAAAG+TGG 0.620698 4_1:+68848680 Msa0567530:CDS
ATTCCTAGATATCAAAATGG+AGG 0.624406 4_1:+68847057 Msa0567530:CDS
AAAATGATCAATGCGGAGGG+TGG 0.627548 4_1:-68846650 None:intergenic
CAAAATGCGGCTACATAGGA+CGG 0.632260 4_1:+68846212 Msa0567530:CDS
AGAATTGTAGTTAAGATGGT+GGG 0.638014 4_1:-68845079 None:intergenic
ATGCGGCTACATAGGACGGT+CGG 0.639956 4_1:+68846216 Msa0567530:CDS
GCATAAAATGATCAATGCGG+AGG 0.642568 4_1:-68846654 None:intergenic
ACAGGAGGTGAAACTAGAGA+TGG 0.647018 4_1:+68848926 Msa0567530:CDS
AAGAAAGCACGACAAATGTG+AGG 0.659530 4_1:-68846453 None:intergenic
AAGCTTGAAAACTTTCCAGG+TGG 0.660259 4_1:+68846277 Msa0567530:CDS
CATCTAATCAAGAAAGCTGG+TGG 0.660897 4_1:+68846614 Msa0567530:CDS
AACCACTTATAATGTGCACA+AGG 0.667560 4_1:+68845570 Msa0567530:CDS
CCTCCCTATAGACTTCAACT+CGG 0.675286 4_1:+68846339 Msa0567530:CDS
GGGCTGATCTACGACGAGTG+TGG 0.675575 4_1:-68848973 None:intergenic
TAATGCTGAGTACTGTTCTG+TGG 0.676472 4_1:-68845109 None:intergenic
CCGAGTTGAAGTCTATAGGG+AGG 0.687521 4_1:-68846339 None:intergenic
CACTATGCAAGACATAGATG+TGG 0.688304 4_1:+68847292 Msa0567530:CDS
TCTGCAACAAGAACTTACAT+GGG 0.692050 4_1:-68846930 None:intergenic
AAAGTCTGTTGGGATTCGAG+GGG 0.692715 4_1:-68845530 None:intergenic
AAGACATAGATGTGGTACAA+CGG 0.695512 4_1:+68847300 Msa0567530:CDS
TTCCATGTAAAACCAACAGG+AGG 0.698959 4_1:+68848911 Msa0567530:CDS
TACCCCTTGATATCAAAATG+CGG 0.703247 4_1:+68846199 Msa0567530:CDS
TGAACCAAAGTATACAACGC+AGG 0.706992 4_1:+68848616 Msa0567530:CDS
TCCGCATAAAATGATCAATG+CGG 0.719094 4_1:-68846657 None:intergenic
TATGGTGTTAGAAGATGCTG+AGG 0.726855 4_1:+68847020 Msa0567530:CDS
CATACTCGATCATTTCACGA+TGG 0.744904 4_1:+68847488 Msa0567530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTATCTTATTAAAAA+TGG - chr4_1:68848441-68848460 None:intergenic 10.0%
!! TTTAACTGCATAAAATATAA+AGG - chr4_1:68845387-68845406 None:intergenic 15.0%
!!! TATTTTCTCTGTTTATTTTT+TGG + chr4_1:68845484-68845503 Msa0567530:intron 15.0%
!!! TTTATATTTTTCCATTTGTA+AGG - chr4_1:68848142-68848161 None:intergenic 15.0%
!!! ATATAAAGGATTCTATTTCT+GGG - chr4_1:68845373-68845392 None:intergenic 20.0%
!! AATATAAAGGATTCTATTTC+TGG - chr4_1:68845374-68845393 None:intergenic 20.0%
!! ACTCATACAAAAATAACTAT+TGG - chr4_1:68845460-68845479 None:intergenic 20.0%
!!! AATGATTTTCTGCATTTATA+AGG - chr4_1:68845616-68845635 None:intergenic 20.0%
!!! TGGTTTTCAAAAAAATTCTA+AGG - chr4_1:68846063-68846082 None:intergenic 20.0%
!! CAACATATAAAAGATTAAGT+TGG - chr4_1:68846160-68846179 None:intergenic 20.0%
!!! CAACTTAATCTTTTATATGT+TGG + chr4_1:68846158-68846177 Msa0567530:intron 20.0%
!!! ACAATTCTAAAATTCTGTTA+TGG + chr4_1:68846316-68846335 Msa0567530:CDS 20.0%
!! TTAATTCCTAGATATCAAAA+TGG + chr4_1:68847054-68847073 Msa0567530:CDS 20.0%
!! TGCGAAAAATAAAAAATAAC+CGG + chr4_1:68847171-68847190 Msa0567530:intron 20.0%
!! AACATTGTAATTGAAAGATT+TGG - chr4_1:68847440-68847459 None:intergenic 20.0%
!! ACAAGAAAATAGGAATTAAA+AGG - chr4_1:68847550-68847569 None:intergenic 20.0%
!!! GATTTCTTTTTCAAAGTTAA+AGG - chr4_1:68848015-68848034 None:intergenic 20.0%
!!! TTTTATTTCTAAATCACAGT+AGG - chr4_1:68848300-68848319 None:intergenic 20.0%
!! TATCTTATTAAAAATGGAGT+TGG - chr4_1:68848435-68848454 None:intergenic 20.0%
! GCATAAAAAATGAACATTCA+AGG + chr4_1:68845343-68845362 Msa0567530:CDS 25.0%
!!! TCTCTGTTTATTTTTTGGTT+TGG + chr4_1:68845489-68845508 Msa0567530:intron 25.0%
!!! CTCTGTTTATTTTTTGGTTT+GGG + chr4_1:68845490-68845509 Msa0567530:intron 25.0%
!!! CCATGTTTTTATTCTAATTC+TGG - chr4_1:68846083-68846102 None:intergenic 25.0%
! CCAGAATTAGAATAAAAACA+TGG + chr4_1:68846080-68846099 Msa0567530:intron 25.0%
!!! TTTTTTATTTTTCGCAGAGT+TGG - chr4_1:68847167-68847186 None:intergenic 25.0%
! GAAAAATAAAAAATAACCGG+AGG + chr4_1:68847174-68847193 Msa0567530:intron 25.0%
! AAGATAGAAATTAGTGTCTT+TGG - chr4_1:68847213-68847232 None:intergenic 25.0%
!! ATTCAATATTAGTAGGCTTT+TGG + chr4_1:68847385-68847404 Msa0567530:CDS 25.0%
! TGAATTGAACACAAGAAAAT+AGG - chr4_1:68847560-68847579 None:intergenic 25.0%
!! TATTTTCTTGTGTTCAATTC+AGG + chr4_1:68847559-68847578 Msa0567530:intron 25.0%
! GGAATTCTTAAAACTCTATT+TGG - chr4_1:68847836-68847855 None:intergenic 25.0%
!!! TGTCATCATTTTTTTCTTCA+TGG - chr4_1:68847857-68847876 None:intergenic 25.0%
! AAAGAAGAAACTTCTTTAGA+AGG + chr4_1:68848078-68848097 Msa0567530:intron 25.0%
AGAATTGTAGTTAAGATGGT+GGG - chr4_1:68845082-68845101 None:intergenic 30.0%
TAGAATTGTAGTTAAGATGG+TGG - chr4_1:68845083-68845102 None:intergenic 30.0%
GTGTAGAATTGTAGTTAAGA+TGG - chr4_1:68845086-68845105 None:intergenic 30.0%
! TCTGGTTTCATATATCAGTT+AGG + chr4_1:68845146-68845165 Msa0567530:five_prime_UTR 30.0%
AAACATGAAATCACTTCAAG+TGG + chr4_1:68845224-68845243 Msa0567530:exon 30.0%
CTTGAATTGAAAAGTCTGTT+GGG - chr4_1:68845543-68845562 None:intergenic 30.0%
ACTTGAATTGAAAAGTCTGT+TGG - chr4_1:68845544-68845563 None:intergenic 30.0%
! TTTTACTGAGCTAAACAAGA+AGG - chr4_1:68845641-68845660 None:intergenic 30.0%
TTGTGAAAATCTATCTCCAT+GGG + chr4_1:68846507-68846526 Msa0567530:CDS 30.0%
ATACATTTACCAATGATCTC+TGG - chr4_1:68846715-68846734 None:intergenic 30.0%
ATTCCTAGATATCAAAATGG+AGG + chr4_1:68847057-68847076 Msa0567530:CDS 30.0%
!! TCATTTTTCTCCTGTCATAT+TGG + chr4_1:68847114-68847133 Msa0567530:intron 30.0%
CCTACTAATATTGAATTTCC+CGG - chr4_1:68847381-68847400 None:intergenic 30.0%
!! TTATTTTGCAGAGTCTTCTA+CGG - chr4_1:68847675-68847694 None:intergenic 30.0%
!! TGTGTTGTTGTGTTTTTCAA+AGG - chr4_1:68847924-68847943 None:intergenic 30.0%
ATTGAAGATCTGAACCAATT+TGG + chr4_1:68847944-68847963 Msa0567530:intron 30.0%
TTGAAGATCTGAACCAATTT+GGG + chr4_1:68847945-68847964 Msa0567530:intron 30.0%
! AAACTTCTTTAGAAGGAAAG+AGG + chr4_1:68848085-68848104 Msa0567530:intron 30.0%
! AACTTCTTTAGAAGGAAAGA+GGG + chr4_1:68848086-68848105 Msa0567530:intron 30.0%
GTACAAATGATCCTTACAAA+TGG + chr4_1:68848128-68848147 Msa0567530:intron 30.0%
! ACTCTATGACACATGATTTT+GGG - chr4_1:68848206-68848225 None:intergenic 30.0%
TGTATAGAGGAAATACTTTG+CGG - chr4_1:68848255-68848274 None:intergenic 30.0%
! ACACAAACTTTTGTGTATAG+AGG - chr4_1:68848268-68848287 None:intergenic 30.0%
AATTCCACAAAACAACATTG+TGG + chr4_1:68848459-68848478 Msa0567530:intron 30.0%
ATTAATTTAGACAGCGTGTC+TGG + chr4_1:68845128-68845147 Msa0567530:five_prime_UTR 35.0%
GCTATTGTGACATGTTTGTT+GGG - chr4_1:68845318-68845337 None:intergenic 35.0%
AGCTATTGTGACATGTTTGT+TGG - chr4_1:68845319-68845338 None:intergenic 35.0%
! AGACTTTTCAATTCAAGTCC+AGG + chr4_1:68845546-68845565 Msa0567530:CDS 35.0%
AACCACTTATAATGTGCACA+AGG + chr4_1:68845570-68845589 Msa0567530:CDS 35.0%
TACCTTGTGCACATTATAAG+TGG - chr4_1:68845575-68845594 None:intergenic 35.0%
! AAGAGCACAATCCTGTTAAT+AGG + chr4_1:68845768-68845787 Msa0567530:intron 35.0%
AGAGCACAATCCTGTTAATA+GGG + chr4_1:68845769-68845788 Msa0567530:intron 35.0%
GATTTATGCTCCCTATTAAC+AGG - chr4_1:68845782-68845801 None:intergenic 35.0%
! ACCTCTTTTGGATAGAGAAA+GGG + chr4_1:68845873-68845892 Msa0567530:intron 35.0%
!! TGTTTTGCATGCTTTTAAGG+AGG - chr4_1:68845953-68845972 None:intergenic 35.0%
!!! TGCTGTTTTGCATGCTTTTA+AGG - chr4_1:68845956-68845975 None:intergenic 35.0%
TACCCCTTGATATCAAAATG+CGG + chr4_1:68846199-68846218 Msa0567530:CDS 35.0%
!! TAGCCGCATTTTGATATCAA+GGG - chr4_1:68846205-68846224 None:intergenic 35.0%
ATATCAAAATGCGGCTACAT+AGG + chr4_1:68846208-68846227 Msa0567530:CDS 35.0%
AGCCTCTAATCTCAAATTCT+GGG + chr4_1:68846245-68846264 Msa0567530:CDS 35.0%
! TTCCCAGAATTTGAGATTAG+AGG - chr4_1:68846250-68846269 None:intergenic 35.0%
! TATCCGAGTTGAAGTCTATA+GGG - chr4_1:68846345-68846364 None:intergenic 35.0%
! TTATCCGAGTTGAAGTCTAT+AGG - chr4_1:68846346-68846365 None:intergenic 35.0%
ATGACTGAAGAACTAGAAGA+TGG + chr4_1:68846403-68846422 Msa0567530:CDS 35.0%
CTTGTGAAAATCTATCTCCA+TGG + chr4_1:68846506-68846525 Msa0567530:CDS 35.0%
! ATTTGGATATTTTCAGCCCA+TGG - chr4_1:68846526-68846545 None:intergenic 35.0%
CACAGCATCTTCTAACAATT+TGG - chr4_1:68846543-68846562 None:intergenic 35.0%
CATAAAATGATCAATGCGGA+GGG - chr4_1:68846656-68846675 None:intergenic 35.0%
!! TCCGCATTGATCATTTTATG+CGG + chr4_1:68846656-68846675 Msa0567530:CDS 35.0%
TCCGCATAAAATGATCAATG+CGG - chr4_1:68846660-68846679 None:intergenic 35.0%
TGCGGATAATTTCAGCAATT+AGG + chr4_1:68846674-68846693 Msa0567530:CDS 35.0%
GCGGATAATTTCAGCAATTA+GGG + chr4_1:68846675-68846694 Msa0567530:CDS 35.0%
AATTTCAGCAATTAGGGCAA+AGG + chr4_1:68846681-68846700 Msa0567530:CDS 35.0%
ATTTCAGCAATTAGGGCAAA+GGG + chr4_1:68846682-68846701 Msa0567530:CDS 35.0%
TTTACCAATGATCTCTGGTT+TGG - chr4_1:68846710-68846729 None:intergenic 35.0%
GAATGTATGAGGAGTTAGAA+GGG + chr4_1:68846764-68846783 Msa0567530:CDS 35.0%
GAGGAGGAATTATGCTTATT+AGG - chr4_1:68846897-68846916 None:intergenic 35.0%
TCTGCAACAAGAACTTACAT+GGG - chr4_1:68846933-68846952 None:intergenic 35.0%
ATCTGCAACAAGAACTTACA+TGG - chr4_1:68846934-68846953 None:intergenic 35.0%
!! TGACCTCCATTTTGATATCT+AGG - chr4_1:68847063-68847082 None:intergenic 35.0%
AGATATCAAAATGGAGGTCA+AGG + chr4_1:68847063-68847082 Msa0567530:CDS 35.0%
AAATGGAGGTCAAGGAAAAA+TGG + chr4_1:68847071-68847090 Msa0567530:CDS 35.0%
TTCTCCTGTCATATTGGAAT+TGG + chr4_1:68847120-68847139 Msa0567530:intron 35.0%
AATGCCAATTCCAATATGAC+AGG - chr4_1:68847127-68847146 None:intergenic 35.0%
AAGACATAGATGTGGTACAA+CGG + chr4_1:68847300-68847319 Msa0567530:CDS 35.0%
CCGGGAAATTCAATATTAGT+AGG + chr4_1:68847378-68847397 Msa0567530:CDS 35.0%
TGAAATGATCGAGTATGTTC+TGG - chr4_1:68847485-68847504 None:intergenic 35.0%
GAATTAAAAGGCTGACCTTT+AGG - chr4_1:68847538-68847557 None:intergenic 35.0%
!! GATCTTTGGAACTTAGTCTT+CGG + chr4_1:68847788-68847807 Msa0567530:intron 35.0%
TGAAGATCTGAACCAATTTG+GGG + chr4_1:68847946-68847965 Msa0567530:intron 35.0%
AACCAAACAGCAAGACTAAT+TGG + chr4_1:68848160-68848179 Msa0567530:intron 35.0%
!! TTCCAATTAGTCTTGCTGTT+TGG - chr4_1:68848165-68848184 None:intergenic 35.0%
! CTCTATGACACATGATTTTG+GGG - chr4_1:68848205-68848224 None:intergenic 35.0%
! CACTCTATGACACATGATTT+TGG - chr4_1:68848207-68848226 None:intergenic 35.0%
ATCATGTGTCATAGAGTGAA+AGG + chr4_1:68848209-68848228 Msa0567530:intron 35.0%
TCATGTGTCATAGAGTGAAA+GGG + chr4_1:68848210-68848229 Msa0567530:intron 35.0%
!!! AGATCCACAATGTTGTTTTG+TGG - chr4_1:68848466-68848485 None:intergenic 35.0%
ACAAAACAACATTGTGGATC+TGG + chr4_1:68848465-68848484 Msa0567530:intron 35.0%
!! TGGACATATTAGCTCTGAAA+AGG - chr4_1:68848566-68848585 None:intergenic 35.0%
CAGAGCTAATATGTCCATAA+TGG + chr4_1:68848569-68848588 Msa0567530:intron 35.0%
TCTAAGCTGAATCACCATTA+TGG - chr4_1:68848586-68848605 None:intergenic 35.0%
TGTCTATAGTTTCAGATGTC+TGG - chr4_1:68848730-68848749 None:intergenic 35.0%
GACAAACCAAAGAATCTATC+AGG + chr4_1:68848854-68848873 Msa0567530:CDS 35.0%
TATCAGGCTGGAGTTTAAAT+TGG + chr4_1:68848870-68848889 Msa0567530:CDS 35.0%
! TAATGCTGAGTACTGTTCTG+TGG - chr4_1:68845112-68845131 None:intergenic 40.0%
! TTCTGTCAGACTGACTTTCA+GGG - chr4_1:68845263-68845282 None:intergenic 40.0%
!!! TTTTTGGTTTGGGCTTATGC+AGG + chr4_1:68845500-68845519 Msa0567530:intron 40.0%
AAAAGTCTGTTGGGATTCGA+GGG - chr4_1:68845534-68845553 None:intergenic 40.0%
GCACATTATAAGTGGTTTCC+TGG - chr4_1:68845567-68845586 None:intergenic 40.0%
TTCCAAACAGTATCATCAGC+AGG + chr4_1:68845664-68845683 Msa0567530:intron 40.0%
TCCAAACAGTATCATCAGCA+GGG + chr4_1:68845665-68845684 Msa0567530:intron 40.0%
TATCATCAGCAGGGTATATG+AGG + chr4_1:68845674-68845693 Msa0567530:intron 40.0%
ATCATCAGCAGGGTATATGA+GGG + chr4_1:68845675-68845694 Msa0567530:intron 40.0%
CACAAACACACTAGGAAGAA+AGG - chr4_1:68845812-68845831 None:intergenic 40.0%
CTTTCTCTATCCAAAAGAGG+TGG - chr4_1:68845874-68845893 None:intergenic 40.0%
! CACCTCTTTTGGATAGAGAA+AGG + chr4_1:68845872-68845891 Msa0567530:intron 40.0%
! CCTCTTTTGGATAGAGAAAG+GGG + chr4_1:68845874-68845893 Msa0567530:intron 40.0%
! CTCTTTTGGATAGAGAAAGG+GGG + chr4_1:68845875-68845894 Msa0567530:intron 40.0%
TAGACAGAGAACATCAGACA+TGG + chr4_1:68845918-68845937 Msa0567530:intron 40.0%
AGAGAACATCAGACATGGAT+AGG + chr4_1:68845923-68845942 Msa0567530:intron 40.0%
GAGAACATCAGACATGGATA+GGG + chr4_1:68845924-68845943 Msa0567530:intron 40.0%
!! AGCCGCATTTTGATATCAAG+GGG - chr4_1:68846204-68846223 None:intergenic 40.0%
!! GTAGCCGCATTTTGATATCA+AGG - chr4_1:68846206-68846225 None:intergenic 40.0%
CAGCCTCTAATCTCAAATTC+TGG + chr4_1:68846244-68846263 Msa0567530:CDS 40.0%
CTCAAGCTTGAAAACTTTCC+AGG + chr4_1:68846274-68846293 Msa0567530:CDS 40.0%
AAGCTTGAAAACTTTCCAGG+TGG + chr4_1:68846277-68846296 Msa0567530:CDS 40.0%
TCAAATGTTTCACATCCACC+TGG - chr4_1:68846295-68846314 None:intergenic 40.0%
! AAGAAAGCACGACAAATGTG+AGG - chr4_1:68846456-68846475 None:intergenic 40.0%
CATTTGTCGTGCTTTCTTCA+TGG + chr4_1:68846458-68846477 Msa0567530:CDS 40.0%
GATGCTGTGATTCTATAGCT+TGG + chr4_1:68846554-68846573 Msa0567530:CDS 40.0%
CTGTGATTCTATAGCTTGGA+AGG + chr4_1:68846558-68846577 Msa0567530:CDS 40.0%
CAGCATCTAATCAAGAAAGC+TGG + chr4_1:68846611-68846630 Msa0567530:CDS 40.0%
CATCTAATCAAGAAAGCTGG+TGG + chr4_1:68846614-68846633 Msa0567530:CDS 40.0%
GCATAAAATGATCAATGCGG+AGG - chr4_1:68846657-68846676 None:intergenic 40.0%
TCATGCAATATGCCAAGAGA+TGG + chr4_1:68846734-68846753 Msa0567530:CDS 40.0%
TCTAACTCCTCATACATTCC+TGG - chr4_1:68846763-68846782 None:intergenic 40.0%
GGAATGTATGAGGAGTTAGA+AGG + chr4_1:68846763-68846782 Msa0567530:CDS 40.0%
TTAGAAGGGCTTAGAGGATA+TGG + chr4_1:68846778-68846797 Msa0567530:CDS 40.0%
TAGAAGGGCTTAGAGGATAT+GGG + chr4_1:68846779-68846798 Msa0567530:CDS 40.0%
TAGACATAGCAAGGAGCAAA+CGG + chr4_1:68846858-68846877 Msa0567530:CDS 40.0%
CTTGTTGCAGATGCTAAAGA+TGG + chr4_1:68846942-68846961 Msa0567530:CDS 40.0%
TCAGACCTTGAAAAGAGAGT+TGG + chr4_1:68846997-68847016 Msa0567530:CDS 40.0%
! CCATACCAACTCTCTTTTCA+AGG - chr4_1:68847005-68847024 None:intergenic 40.0%
!! CCTTGAAAAGAGAGTTGGTA+TGG + chr4_1:68847002-68847021 Msa0567530:CDS 40.0%
! TATGGTGTTAGAAGATGCTG+AGG + chr4_1:68847020-68847039 Msa0567530:CDS 40.0%
GGAATTGGCATTAATGCTGA+AGG + chr4_1:68847135-68847154 Msa0567530:intron 40.0%
!! TGGTCATTTTCATAAGCCTC+CGG - chr4_1:68847193-68847212 None:intergenic 40.0%
! TCTTGCATAGTGCAGTTTTC+GGG - chr4_1:68847284-68847303 None:intergenic 40.0%
! GTCTTGCATAGTGCAGTTTT+CGG - chr4_1:68847285-68847304 None:intergenic 40.0%
CACTATGCAAGACATAGATG+TGG + chr4_1:68847292-68847311 Msa0567530:CDS 40.0%
ATGCAACTGCAACAAAAGTC+CGG + chr4_1:68847359-68847378 Msa0567530:CDS 40.0%
GCAGTAATTCAGCTAAGACT+TGG - chr4_1:68847463-68847482 None:intergenic 40.0%
CATACTCGATCATTTCACGA+TGG + chr4_1:68847488-68847507 Msa0567530:CDS 40.0%
TAAAAGGCTGACCTTTAGGT+AGG - chr4_1:68847534-68847553 None:intergenic 40.0%
GACTAAGTTCCAAAGATCCA+GGG - chr4_1:68847786-68847805 None:intergenic 40.0%
AGACTAAGTTCCAAAGATCC+AGG - chr4_1:68847787-68847806 None:intergenic 40.0%
GGTATTTAAGCAGATGCTAG+TGG - chr4_1:68847903-68847922 None:intergenic 40.0%
GAAAACATCTGACCCCAAAT+TGG - chr4_1:68847961-68847980 None:intergenic 40.0%
! AGTTTTCCATCTGCATGGAA+GGG - chr4_1:68848037-68848056 None:intergenic 40.0%
! TAGTTTTCCATCTGCATGGA+AGG - chr4_1:68848038-68848057 None:intergenic 40.0%
!!! TCACTAGTTTTCCATCTGCA+TGG - chr4_1:68848042-68848061 None:intergenic 40.0%
!! ATGACACATGATTTTGGGGA+GGG - chr4_1:68848201-68848220 None:intergenic 40.0%
!! TATGACACATGATTTTGGGG+AGG - chr4_1:68848202-68848221 None:intergenic 40.0%
! GTGGATCTGGTTCACATTAT+AGG + chr4_1:68848478-68848497 Msa0567530:intron 40.0%
! TCTGGTTCACATTATAGGCA+TGG + chr4_1:68848483-68848502 Msa0567530:intron 40.0%
! TGCGTTGTATACTTTGGTTC+AGG - chr4_1:68848617-68848636 None:intergenic 40.0%
TGAACCAAAGTATACAACGC+AGG + chr4_1:68848616-68848635 Msa0567530:CDS 40.0%
AGAACCTGCGTTGTATACTT+TGG - chr4_1:68848623-68848642 None:intergenic 40.0%
AAGTGGAATCAACTCTCAAC+AGG + chr4_1:68848697-68848716 Msa0567530:CDS 40.0%
CGAGAATGTAAAGTCCAACA+TGG + chr4_1:68848772-68848791 Msa0567530:CDS 40.0%
AACCAAAGAATCTATCAGGC+TGG + chr4_1:68848858-68848877 Msa0567530:CDS 40.0%
TCCTTCCATGTAAAACCAAC+AGG + chr4_1:68848908-68848927 Msa0567530:CDS 40.0%
!!! TCCTGTTGGTTTTACATGGA+AGG - chr4_1:68848912-68848931 None:intergenic 40.0%
TTCCATGTAAAACCAACAGG+AGG + chr4_1:68848911-68848930 Msa0567530:CDS 40.0%
GAGCTAATTGCTGCAAGCTT+GGG - chr4_1:68845198-68845217 None:intergenic 45.0%
TGAGCTAATTGCTGCAAGCT+TGG - chr4_1:68845199-68845218 None:intergenic 45.0%
! ACTTTCAGGGTCTTGTGCTT+GGG - chr4_1:68845250-68845269 None:intergenic 45.0%
! GTTCTGTCAGACTGACTTTC+AGG - chr4_1:68845264-68845283 None:intergenic 45.0%
AAAGTCTGTTGGGATTCGAG+GGG - chr4_1:68845533-68845552 None:intergenic 45.0%
GAAAAGTCTGTTGGGATTCG+AGG - chr4_1:68845535-68845554 None:intergenic 45.0%
ACCCTGCTGATGATACTGTT+TGG - chr4_1:68845669-68845688 None:intergenic 45.0%
CACACACACACAAACACACT+AGG - chr4_1:68845820-68845839 None:intergenic 45.0%
TCTATCCAAAAGAGGTGGTG+GGG - chr4_1:68845869-68845888 None:intergenic 45.0%
CTCTATCCAAAAGAGGTGGT+GGG - chr4_1:68845870-68845889 None:intergenic 45.0%
TCTCTATCCAAAAGAGGTGG+TGG - chr4_1:68845871-68845890 None:intergenic 45.0%
CCCCTTTCTCTATCCAAAAG+AGG - chr4_1:68845877-68845896 None:intergenic 45.0%
GGGAAAATTTCCCTCAAGAG+AGG + chr4_1:68845895-68845914 Msa0567530:intron 45.0%
TCTCTGTCTACCTCTCTTGA+GGG - chr4_1:68845908-68845927 None:intergenic 45.0%
TTCTCTGTCTACCTCTCTTG+AGG - chr4_1:68845909-68845928 None:intergenic 45.0%
CAAAATGCGGCTACATAGGA+CGG + chr4_1:68846212-68846231 Msa0567530:CDS 45.0%
CCTCCCTATAGACTTCAACT+CGG + chr4_1:68846339-68846358 Msa0567530:CDS 45.0%
TGTGAGGAAAGCTTCAGTCT+TGG - chr4_1:68846440-68846459 None:intergenic 45.0%
TAAAAATGAGCCCGCAAGTG+AGG + chr4_1:68846585-68846604 Msa0567530:CDS 45.0%
AAGCTGGTGGTTCGATGATT+TGG + chr4_1:68846627-68846646 Msa0567530:CDS 45.0%
AAAATGATCAATGCGGAGGG+TGG - chr4_1:68846653-68846672 None:intergenic 45.0%
GGAACCAAACCAGAGATCAT+TGG + chr4_1:68846703-68846722 Msa0567530:CDS 45.0%
ATGGCTACCAGGAATGTATG+AGG + chr4_1:68846753-68846772 Msa0567530:CDS 45.0%
!! AGAGAGCACTAGACATAGCA+AGG + chr4_1:68846849-68846868 Msa0567530:CDS 45.0%
!!! GTGCAGTTTTCGGGTGAATT+AGG - chr4_1:68847275-68847294 None:intergenic 45.0%
TGCAACTGCAACAAAAGTCC+GGG + chr4_1:68847360-68847379 Msa0567530:CDS 45.0%
GATCTGTTGCAACTTCAGCT+AGG - chr4_1:68847418-68847437 None:intergenic 45.0%
AGCCATTGACACCTACCTAA+AGG + chr4_1:68847520-68847539 Msa0567530:CDS 45.0%
GACCTTTAGGTAGGTGTCAA+TGG - chr4_1:68847525-68847544 None:intergenic 45.0%
TTGGTCAGTTAGGGAAGGAT+GGG - chr4_1:68847650-68847669 None:intergenic 45.0%
TCTACGGTCTTGGTCAGTTA+GGG - chr4_1:68847659-68847678 None:intergenic 45.0%
TTCTACGGTCTTGGTCAGTT+AGG - chr4_1:68847660-68847679 None:intergenic 45.0%
ACCTGGACAACTGTTCTTAG+AGG - chr4_1:68847757-68847776 None:intergenic 45.0%
AAGATCCAGGGAACTTTACC+TGG - chr4_1:68847774-68847793 None:intergenic 45.0%
GGTAAAGTTCCCTGGATCTT+TGG + chr4_1:68847774-68847793 Msa0567530:intron 45.0%
AGAAATCCCTTCCATGCAGA+TGG + chr4_1:68848028-68848047 Msa0567530:intron 45.0%
! GCATGGCATCTAAGATAGTG+TGG + chr4_1:68848500-68848519 Msa0567530:intron 45.0%
AGCAATGCAAGAGAAGGAAC+CGG + chr4_1:68848670-68848689 Msa0567530:CDS 45.0%
! GAGTTGATTCCACTTTGTGC+CGG - chr4_1:68848692-68848711 None:intergenic 45.0%
GTTCTGCAGTTCTACCATGT+TGG - chr4_1:68848789-68848808 None:intergenic 45.0%
CTCCAGCCTGATAGATTCTT+TGG - chr4_1:68848863-68848882 None:intergenic 45.0%
CAGGCTGGAGTTTAAATTGG+CGG + chr4_1:68848873-68848892 Msa0567530:CDS 45.0%
!!! CACCTCCTGTTGGTTTTACA+TGG - chr4_1:68848916-68848935 None:intergenic 45.0%
! TCTCTAGTTTCACCTCCTGT+TGG - chr4_1:68848926-68848945 None:intergenic 45.0%
ACAGGAGGTGAAACTAGAGA+TGG + chr4_1:68848926-68848945 Msa0567530:CDS 45.0%
! GACTTTCAGGGTCTTGTGCT+TGG - chr4_1:68845251-68845270 None:intergenic 50.0%
! CCGAGTTGAAGTCTATAGGG+AGG - chr4_1:68846342-68846361 None:intergenic 50.0%
AGATGCTGCATCCTCACTTG+CGG - chr4_1:68846599-68846618 None:intergenic 50.0%
TATGCCAAGAGATGGCTACC+AGG + chr4_1:68846742-68846761 Msa0567530:CDS 50.0%
CATTCCTGGTAGCCATCTCT+TGG - chr4_1:68846749-68846768 None:intergenic 50.0%
GAGGAGTTAGAAGGGCTTAG+AGG + chr4_1:68846772-68846791 Msa0567530:CDS 50.0%
CATGGGACAGCATCTTGTTG+AGG - chr4_1:68846916-68846935 None:intergenic 50.0%
CTTGGTCAGTTAGGGAAGGA+TGG - chr4_1:68847651-68847670 None:intergenic 50.0%
TGCAGAGTCTTCTACGGTCT+TGG - chr4_1:68847669-68847688 None:intergenic 50.0%
! GCCTCTAAGAACAGTTGTCC+AGG + chr4_1:68847753-68847772 Msa0567530:CDS 50.0%
GTTGTCCAGGTAAAGTTCCC+TGG + chr4_1:68847766-68847785 Msa0567530:intron 50.0%
GAGAGCAGCAATGCAAGAGA+AGG + chr4_1:68848664-68848683 Msa0567530:CDS 50.0%
!! CGAGTGTGGTTTGGTGATGT+GGG - chr4_1:68848962-68848981 None:intergenic 50.0%
!! ACGAGTGTGGTTTGGTGATG+TGG - chr4_1:68848963-68848982 None:intergenic 50.0%
GATCTACGACGAGTGTGGTT+TGG - chr4_1:68848971-68848990 None:intergenic 50.0%
!! TCCAAAAGAGGTGGTGGGGT+GGG - chr4_1:68845865-68845884 None:intergenic 55.0%
!! ATCCAAAAGAGGTGGTGGGG+TGG - chr4_1:68845866-68845885 None:intergenic 55.0%
ATGCGGCTACATAGGACGGT+CGG + chr4_1:68846216-68846235 Msa0567530:CDS 55.0%
GATGCTGCATCCTCACTTGC+GGG - chr4_1:68846598-68846617 None:intergenic 55.0%
GGGCTTAGAGGATATGGGCA+TGG + chr4_1:68846784-68846803 Msa0567530:CDS 55.0%
GGGACAGCATCTTGTTGAGG+AGG - chr4_1:68846913-68846932 None:intergenic 55.0%
CGGTCTTGGTCAGTTAGGGA+AGG - chr4_1:68847655-68847674 None:intergenic 55.0%
GAGAAGGAACCGGCACAAAG+TGG + chr4_1:68848680-68848699 Msa0567530:CDS 55.0%
GTAGATCAGCCCCGAGAAGA+AGG + chr4_1:68848984-68849003 Msa0567530:CDS 55.0%
ACACGGAGAGCCTTCTTCTC+GGG - chr4_1:68848997-68849016 None:intergenic 55.0%
GACACGGAGAGCCTTCTTCT+CGG - chr4_1:68848998-68849017 None:intergenic 55.0%
! TCCCACCCCACCACCTCTTT+TGG + chr4_1:68845861-68845880 Msa0567530:intron 60.0%
GGGCTGATCTACGACGAGTG+TGG - chr4_1:68848976-68848995 None:intergenic 60.0%
CACGGAGAGCCTTCTTCTCG+GGG - chr4_1:68848996-68849015 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 68845067 68849021 68845067 ID=Msa0567530;Name=Msa0567530
chr4_1 mRNA 68845067 68849021 68845067 ID=Msa0567530-mRNA-1;Parent=Msa0567530;Name=Msa0567530-mRNA-1;_AED=0.08;_eAED=0.08;_QI=161|0.8|0.83|1|1|1|6|0|641
chr4_1 exon 68845067 68845364 68845067 ID=Msa0567530-mRNA-1:exon:22799;Parent=Msa0567530-mRNA-1
chr4_1 exon 68845522 68845591 68845522 ID=Msa0567530-mRNA-1:exon:22800;Parent=Msa0567530-mRNA-1
chr4_1 exon 68846199 68847097 68846199 ID=Msa0567530-mRNA-1:exon:22801;Parent=Msa0567530-mRNA-1
chr4_1 exon 68847268 68847541 68847268 ID=Msa0567530-mRNA-1:exon:22802;Parent=Msa0567530-mRNA-1
chr4_1 exon 68847646 68847774 68847646 ID=Msa0567530-mRNA-1:exon:22803;Parent=Msa0567530-mRNA-1
chr4_1 exon 68848605 68849021 68848605 ID=Msa0567530-mRNA-1:exon:22804;Parent=Msa0567530-mRNA-1
chr4_1 five_prime_UTR 68845067 68845227 68845067 ID=Msa0567530-mRNA-1:five_prime_utr;Parent=Msa0567530-mRNA-1
chr4_1 CDS 68845228 68845364 68845228 ID=Msa0567530-mRNA-1:cds;Parent=Msa0567530-mRNA-1
chr4_1 CDS 68845522 68845591 68845522 ID=Msa0567530-mRNA-1:cds;Parent=Msa0567530-mRNA-1
chr4_1 CDS 68846199 68847097 68846199 ID=Msa0567530-mRNA-1:cds;Parent=Msa0567530-mRNA-1
chr4_1 CDS 68847268 68847541 68847268 ID=Msa0567530-mRNA-1:cds;Parent=Msa0567530-mRNA-1
chr4_1 CDS 68847646 68847774 68847646 ID=Msa0567530-mRNA-1:cds;Parent=Msa0567530-mRNA-1
chr4_1 CDS 68848605 68849021 68848605 ID=Msa0567530-mRNA-1:cds;Parent=Msa0567530-mRNA-1
Gene Sequence

>Msa0567530

ATGAAATCACTTCAAGTGGCCCAAGCACAAGACCCTGAAAGTCAGTCTGACAGAACTGAACAAGTTTCAGATAAAAGCATAGTTATTCCCAACAAACATGTCACAATAGCTGATAGCATAAAAAATGAACATTCAAGGTTCGTTGCCCCTCGAATCCCAACAGACTTTTCAATTCAAGTCCAGGAAACCACTTATAATGTGCACAAGTACCCCTTGATATCAAAATGCGGCTACATAGGACGGTCGGAAGTACAGCCTCTAATCTCAAATTCTGGGAATGTGCTCAAGCTTGAAAACTTTCCAGGTGGATGTGAAACATTTGAAACAATTCTAAAATTCTGTTATGGCCTCCCTATAGACTTCAACTCGGATAATGTAGCTGCACTAAGATGTGCATCAGAATTTCTAGAGATGACTGAAGAACTAGAAGATGGCAATCTCATTTCCAAGACTGAAGCTTTCCTCACATTTGTCGTGCTTTCTTCATGGAAAGACACTGTTACTGTTCTGAAATCTTGTGAAAATCTATCTCCATGGGCTGAAAATATCCAAATTGTTAGAAGATGCTGTGATTCTATAGCTTGGAAGGCTACTAAAAATGAGCCCGCAAGTGAGGATGCAGCATCTAATCAAGAAAGCTGGTGGTTCGATGATTTGGCCACCCTCCGCATTGATCATTTTATGCGGATAATTTCAGCAATTAGGGCAAAGGGAACCAAACCAGAGATCATTGGTAAATGTATCATGCAATATGCCAAGAGATGGCTACCAGGAATGTATGAGGAGTTAGAAGGGCTTAGAGGATATGGGCATGGAAAAAGTAATCTACAGTTCACTATATTTAGCATGAAGAAAGAAGAGAGCACTAGACATAGCAAGGAGCAAACGGCTATTATTGAAAGCCTAATAAGCATAATTCCTCCTCAACAAGATGCTGTCCCATGTAAGTTCTTGTTGCAGATGCTAAAGATGGCAATGATGTATTCTGTATCATCAGCTCTCATTTCAGACCTTGAAAAGAGAGTTGGTATGGTGTTAGAAGATGCTGAGGTGAATGATCTCTTAATTCCTAGATATCAAAATGGAGGTCAAGGAAAAATGGTCAACATGCCTAATTCACCCGAAAACTGCACTATGCAAGACATAGATGTGGTACAACGGATTGTTGAATACTTCTTGATGCATGAAAAACAGCAAATGCAACTGCAACAAAAGTCCGGGAAATTCAATATTAGTAGGCTTTTGGACAATTACCTAGCTGAAGTTGCAACAGATCCAAATCTTTCAATTACAATGTTCCAAGTCTTAGCTGAATTACTGCCAGAACATACTCGATCATTTCACGATGGTCTATATAGAGCCATTGACACCTACCTAAAGACCCATCCTTCCCTAACTGACCAAGACCGTAGAAGACTCTGCAAAATAATGAACTGTGAAAAACTCTCACTTGATGCATGCATACATGCTGCACAAAATGATAGATTGCCTCTAAGAACAGTTGTCCAGAAATTGTCACCTGAACCAAAGTATACAACGCAGGTTCTATTTGCAGAGCAAGTAAAGATGAGAGCAGCAATGCAAGAGAAGGAACCGGCACAAAGTGGAATCAACTCTCAACAGGATGTAAACCAGACATCTGAAACTATAGACATAAAAACACTCAAAGCAGAACTCGAGAATGTAAAGTCCAACATGGTAGAACTGCAGAACGACTACTTTGAGTTGCAAGAAGAGTATAAAAAGTTAACTAGCAACGACAAACCAAAGAATCTATCAGGCTGGAGTTTAAATTGGCGGAAGATCAAGAAATCCTTCCATGTAAAACCAACAGGAGGTGAAACTAGAGATGGACAAGATATGCCCACATCACCAAACCACACTCGTCGTAGATCAGCCCCGAGAAGAAGGCTCTCCGTGTCTTAA
Protein sequence

>Msa0567530

MKSLQVAQAQDPESQSDRTEQVSDKSIVIPNKHVTIADSIKNEHSRFVAPRIPTDFSIQVQETTYNVHKYPLISKCGYIGRSEVQPLISNSGNVLKLENFPGGCETFETILKFCYGLPIDFNSDNVAALRCASEFLEMTEELEDGNLISKTEAFLTFVVLSSWKDTVTVLKSCENLSPWAENIQIVRRCCDSIAWKATKNEPASEDAASNQESWWFDDLATLRIDHFMRIISAIRAKGTKPEIIGKCIMQYAKRWLPGMYEELEGLRGYGHGKSNLQFTIFSMKKEESTRHSKEQTAIIESLISIIPPQQDAVPCKFLLQMLKMAMMYSVSSALISDLEKRVGMVLEDAEVNDLLIPRYQNGGQGKMVNMPNSPENCTMQDIDVVQRIVEYFLMHEKQQMQLQQKSGKFNISRLLDNYLAEVATDPNLSITMFQVLAELLPEHTRSFHDGLYRAIDTYLKTHPSLTDQDRRRLCKIMNCEKLSLDACIHAAQNDRLPLRTVVQKLSPEPKYTTQVLFAEQVKMRAAMQEKEPAQSGINSQQDVNQTSETIDIKTLKAELENVKSNMVELQNDYFELQEEYKKLTSNDKPKNLSGWSLNWRKIKKSFHVKPTGGETRDGQDMPTSPNHTRRRSAPRRRLSVS*