AlfalfaGEDB | Msa0554430

M. sativa cultivar zhongmu-4 / Msa0554430

KEGG: -
GO: -
PFAM: PF03007.19

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0554430	XP_003607487.2	97.263	475	12	1	1	474	1	475	0.0	948

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0554430	sp\|Q9M3B3\|WSD4_ARATH	27.632	456	295	8	7	437	15	460	5.39e-54	192

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0554430	G7JTV5	97.263	475	12	1	1	474	1	475	0.0	948

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000220	Msa0554430	0.813657	2.290971e-51	-8.615850e-47
Msa0001510	Msa0554430	0.802633	5.113636e-49	-8.615850e-47
Msa0001540	Msa0554430	0.805828	1.105395e-49	-8.615850e-47
Msa0002690	Msa0554430	0.846339	2.311552e-59	-8.615850e-47
Msa0002700	Msa0554430	0.849867	2.453603e-60	-8.615850e-47
Msa0003370	Msa0554430	0.808803	2.588157e-50	-8.615850e-47
Msa0004180	Msa0554430	0.807012	6.220913e-50	-8.615850e-47
Msa0005500	Msa0554430	0.851691	7.526996e-61	-8.615850e-47
Msa0006580	Msa0554430	0.820106	8.171713e-53	-8.615850e-47
Msa0007570	Msa0554430	0.809614	1.734834e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0554430	MtrunA17_Chr4g0040231	97.263	475	12	1	1	474	1	475	0.0	948
Msa0554430	MtrunA17_Chr4g0040001	83.298	467	77	1	1	466	1	467	0.0	771
Msa0554430	MtrunA17_Chr4g0040221	80.591	474	88	2	1	474	1	470	0.0	756
Msa0554430	MtrunA17_Chr4g0040011	82.658	444	70	2	1	443	9	446	0.0	716
Msa0554430	MtrunA17_Chr4g0040261	65.517	464	155	3	5	467	37	496	0.0	617
Msa0554430	MtrunA17_Chr4g0040251	61.935	465	171	4	5	467	4	464	0.0	591
Msa0554430	MtrunA17_Chr4g0040271	64.524	420	143	4	5	423	4	418	0.0	557
Msa0554430	MtrunA17_Chr7g0217051	56.710	462	196	3	7	467	8	466	0.0	547
Msa0554430	MtrunA17_Chr7g0248011	45.798	476	248	5	2	474	3	471	1.67e-146	427
Msa0554430	MtrunA17_Chr6g0457271	50.704	355	173	2	67	420	5	358	4.32e-127	374

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0554430	AT3G49190.1	27.632	456	295	8	7	437	15	460	5.48e-55	192
Msa0554430	AT5G53390.1	29.228	479	304	15	9	466	14	478	7.60e-53	185
Msa0554430	AT3G49210.1	26.680	491	321	9	10	466	26	511	8.66e-49	175
Msa0554430	AT3G49200.1	26.106	452	305	9	10	437	26	472	4.46e-47	170
Msa0554430	AT2G38995.2	27.389	471	316	10	10	466	21	479	9.61e-47	169
Msa0554430	AT5G16350.3	28.054	442	290	14	7	437	4	428	1.08e-46	168
Msa0554430	AT1G72110.1	26.752	471	322	8	7	464	9	469	3.10e-46	167
Msa0554430	AT5G12420.1	26.096	456	302	12	5	437	3	446	1.77e-45	165
Msa0554430	AT5G22490.1	27.536	483	303	15	11	466	12	474	2.70e-45	165
Msa0554430	AT5G16350.1	27.233	459	292	14	7	437	4	448	1.07e-44	163

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Find 116 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAATCTATAACACAAC+TGG	0.341009	4_1:+51718563	Msa0554430:CDS
AAAGCGGTGGCACAATTATT+AGG	0.306594	4_1:-51721423	None:intergenic
AAAGGAGTTAAATCAAAGCT+TGG	0.513777	4_1:+51717499	Msa0554430:CDS
AAATAATCTATAACACAACT+GGG	0.623010	4_1:+51718564	Msa0554430:CDS
AACACAATCGAGTAAAATCA+TGG	0.522028	4_1:-51721237	None:intergenic
AACATAATGGACAAATTCTT+TGG	0.247013	4_1:+51716578	Msa0554430:exon
AACATCAAATTCCACAGCGG+TGG	0.618411	4_1:+51718090	Msa0554430:CDS
AACCAGAGTCTAAAGGTCTA+TGG	0.314366	4_1:+51718167	Msa0554430:CDS
AAGATGACAAAACACCTATA+AGG	0.442261	4_1:+51717407	Msa0554430:CDS
AATATATAGAACCATAATAG+AGG	0.500079	4_1:-51716759	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATTTGTCCATTATGTTG+TGG	-	chr4_1:51716577-51716596	None:intergenic	30.0%
ATGGCTAGTGAATTATCAAA+TGG	-	chr4_1:51716695-51716714	None:intergenic	30.0%
CATGATGAATTTATGGTTTC+AGG	-	chr4_1:51717025-51717044	None:intergenic	30.0%
ACCACAAAATAAACCACTTT+GGG	+	chr4_1:51717102-51717121	Msa0554430:CDS	30.0%
ATCTGATATGGAGCTAAAAA+AGG	-	chr4_1:51717353-51717372	None:intergenic	30.0%
AAGATGACAAAACACCTATA+AGG	+	chr4_1:51717407-51717426	Msa0554430:CDS	30.0%
AAAGGAGTTAAATCAAAGCT+TGG	+	chr4_1:51717499-51717518	Msa0554430:CDS	30.0%
AAAAACATCAAATTCCACAG+CGG	+	chr4_1:51718087-51718106	Msa0554430:CDS	30.0%
ATGCTCAACACAAGAAATAT+TGG	+	chr4_1:51718115-51718134	Msa0554430:CDS	30.0%
TATTCCAAACAAACTCAAGA+GGG	-	chr4_1:51718255-51718274	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	51716543	51721588	51716543	ID=Msa0554430;Name=Msa0554430
chr4_1	mRNA	51716543	51721588	51716543	ID=Msa0554430-mRNA-1;Parent=Msa0554430;Name=Msa0554430-mRNA-1;_AED=0.07;_eAED=0.07;_QI=41\|1\|1\|1\|1\|1\|5\|557\|474
chr4_1	exon	51716543	51716769	51716543	ID=Msa0554430-mRNA-1:exon:14876;Parent=Msa0554430-mRNA-1
chr4_1	exon	51716944	51717525	51716944	ID=Msa0554430-mRNA-1:exon:14877;Parent=Msa0554430-mRNA-1
chr4_1	exon	51718013	51718366	51718013	ID=Msa0554430-mRNA-1:exon:14878;Parent=Msa0554430-mRNA-1
chr4_1	exon	51718554	51718691	51718554	ID=Msa0554430-mRNA-1:exon:14879;Parent=Msa0554430-mRNA-1
chr4_1	exon	51720867	51721588	51720867	ID=Msa0554430-mRNA-1:exon:14880;Parent=Msa0554430-mRNA-1
chr4_1	five_prime_UTR	51716543	51716583	51716543	ID=Msa0554430-mRNA-1:five_prime_utr;Parent=Msa0554430-mRNA-1
chr4_1	CDS	51716584	51716769	51716584	ID=Msa0554430-mRNA-1:cds;Parent=Msa0554430-mRNA-1
chr4_1	CDS	51716944	51717525	51716944	ID=Msa0554430-mRNA-1:cds;Parent=Msa0554430-mRNA-1
chr4_1	CDS	51718013	51718366	51718013	ID=Msa0554430-mRNA-1:cds;Parent=Msa0554430-mRNA-1
chr4_1	CDS	51718554	51718691	51718554	ID=Msa0554430-mRNA-1:cds;Parent=Msa0554430-mRNA-1
chr4_1	CDS	51720867	51721031	51720867	ID=Msa0554430-mRNA-1:cds;Parent=Msa0554430-mRNA-1
chr4_1	three_prime_UTR	51721032	51721588	51721032	ID=Msa0554430-mRNA-1:three_prime_utr;Parent=Msa0554430-mRNA-1

Gene Sequence

>Msa0554430

ATGGACAAATTCTTTGGAGAAGTTCAAGAGCCAGTGAGTCCTCATGGACAATATTTCAACAGCTCTGTGATGTGTTCATATGTTTTTGGCTTTCTGGAATTAGCTATTCCATTTGATAATTCACTAGCCATACCTTTGCTCAAAGATGTCTTCATCCCTATCAACCCACGCTTCTCCTCTATTATGGTAAAAGACGAAGATGGTAAGATGAAATGGCAAAAGGTTGAAGTGAAGCCTGAAGAACACTTAAAGATTCCCATATTTCCTGAAACCATAAATTCATCATGTGAATTATATGACAAATATGTTAGTAACTATGTAACAAGTATTCTAAATGGAAGAACACCACAAAATAAACCACTTTGGGAAATTCATATTATCAATTATCCAACAACCAATGCAGCTTGCACCATAATATTCAAACTGAATCATGCACTTGGTGATGGTTACTCTCTCATGAGTGCTCTTCTTTCTTGTCTTCAAAGAGCTGATGATCCTTCTCTACCTCTGTCTTTTCCTTCACAAAGACCACAATTAGATTCAAAATATGCAAAGATAAACTTATTTGACAAGCTATGTTTTGTTATCTCATCCTTTTTTAGCTCCATATCAGATTTTGGATCAAGCATATTGAAGTCTAGAATGATTAAAGATGACAAAACACCTATAAGGTCAGGATATGAAGGTGCTGAATCTCAGCCTTTTATCTTGTCAAACATAACATTATCTCTTGATCAAATCAAAGGAGTTAAATCAAAGCTTGGAGTGACTATAAACGACGTGATTACGGGAATGATCTTCTATGGGATTAGGCTATACATGGAAGAGAAGAATGAGAAGACAAAAACATCAAATTCCACAGCGGTGGTAATGCTCAACACAAGAAATATTGGAGGTTACCAATCAGTGAAGGAAATGCAAAAACCAGAGTCTAAAGGTCTATGGGGGAATAAAATTTCTTTCTTACAAATACCTATACCTAAGATGAGCCAATCAAATATCTCCAACCCTCTTGAGTTTGTTTGGAATACTCGTGAACTAATCAAGAGGAAGAGACGTTCTTTCAGTGTTTATCTCATAGCTTTGCTCTTGGATTTGGAAATGAAGTTAAGAGGGTCTGAGGTGGTAGCTAAAATAATCTATAACACAACTGGGAACTCAAGTGTTCTTATGTCAAACATTGTAGGGCCAGTAGAGAAGATGACTTTGGCAAATCATCCAGTAAATGGATTGTGTTTCACCATGACAAATGGACCTGAGAATGTAAACATTACAATTATTAGCTACATGAATATATTAAGAATCACCTTGAAAACCTTAAAGGGATTCATAGATGAACAAAAATTGAAGTTCTGCATAGAGAAAGCAGTCGAAGTCATATCCGAAGCAGCTATGGAGATCTCCGAGATATCCACAAAAAATTAA

Protein sequence

>Msa0554430

MDKFFGEVQEPVSPHGQYFNSSVMCSYVFGFLELAIPFDNSLAIPLLKDVFIPINPRFSSIMVKDEDGKMKWQKVEVKPEEHLKIPIFPETINSSCELYDKYVSNYVTSILNGRTPQNKPLWEIHIINYPTTNAACTIIFKLNHALGDGYSLMSALLSCLQRADDPSLPLSFPSQRPQLDSKYAKINLFDKLCFVISSFFSSISDFGSSILKSRMIKDDKTPIRSGYEGAESQPFILSNITLSLDQIKGVKSKLGVTINDVITGMIFYGIRLYMEEKNEKTKTSNSTAVVMLNTRNIGGYQSVKEMQKPESKGLWGNKISFLQIPIPKMSQSNISNPLEFVWNTRELIKRKRRSFSVYLIALLLDLEMKLRGSEVVAKIIYNTTGNSSVLMSNIVGPVEKMTLANHPVNGLCFTMTNGPENVNITIISYMNILRITLKTLKGFIDEQKLKFCIEKAVEVISEAAMEISEISTKN*