AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0567420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567420 MtrunA17_Chr8g0382231 97.192 641 7 1 1 641 1 630 0.0 1296
Msa0567420 MtrunA17_Chr4g0050021 41.679 691 290 13 26 641 17 669 1.12e-174 512
Msa0567420 MtrunA17_Chr2g0279481 41.453 702 297 16 13 634 3 670 1.05e-172 508
Msa0567420 MtrunA17_Chr5g0401451 34.966 592 302 12 19 577 10 551 3.99e-114 355
Msa0567420 MtrunA17_Chr1g0191711 34.392 567 312 11 24 581 29 544 1.59e-112 348
Msa0567420 MtrunA17_Chr2g0303541 32.701 633 340 11 37 618 10 607 6.78e-112 350
Msa0567420 MtrunA17_Chr1g0181181 34.535 527 282 11 41 544 15 501 5.27e-102 322
Msa0567420 MtrunA17_Chr2g0285511 32.855 627 321 16 52 633 26 597 1.12e-101 322
Msa0567420 MtrunA17_Chr4g0010091 33.582 536 287 8 20 527 2 496 2.37e-101 321
Msa0567420 MtrunA17_Chr1g0195911 33.733 584 287 13 52 582 36 572 1.03e-100 320
Msa0567420 MtrunA17_Chr4g0053841 35.252 556 274 12 52 570 13 519 1.86e-100 318
Msa0567420 MtrunA17_Chr2g0320691 30.116 601 334 11 44 591 15 582 5.01e-100 318
Msa0567420 MtrunA17_Chr8g0374011 34.735 547 253 11 47 529 15 521 5.70e-99 317
Msa0567420 MtrunA17_Chr5g0401341 32.258 589 282 15 38 563 11 545 9.44e-99 315
Msa0567420 MtrunA17_Chr8g0367891 36.810 489 255 8 55 522 29 484 2.79e-98 313
Msa0567420 MtrunA17_Chr7g0268781 33.333 621 303 16 52 613 36 604 3.49e-98 313
Msa0567420 MtrunA17_Chr1g0150191 32.545 550 269 12 46 546 21 517 9.83e-98 312
Msa0567420 MtrunA17_Chr1g0207461 32.877 584 275 18 42 563 18 546 9.02e-96 307
Msa0567420 MtrunA17_Chr7g0245201 35.225 511 267 9 42 527 51 522 1.75e-94 304
Msa0567420 MtrunA17_Chr5g0412591 32.911 553 254 10 42 539 16 506 5.86e-90 291
Msa0567420 MtrunA17_Chr4g0076521 33.147 537 300 11 45 551 19 526 7.39e-89 288
Msa0567420 MtrunA17_Chr3g0108331 31.177 603 344 14 52 641 24 568 1.13e-88 286
Msa0567420 MtrunA17_Chr1g0199631 30.808 594 306 13 70 611 8 548 1.16e-88 287
Msa0567420 MtrunA17_Chr5g0432861 31.827 531 314 8 52 572 25 517 2.00e-84 276
Msa0567420 MtrunA17_Chr2g0290741 31.197 593 328 14 35 598 8 549 4.21e-82 270
Msa0567420 MtrunA17_Chr4g0003891 35.897 312 154 6 215 523 46 314 1.38e-62 214
Msa0567420 MtrunA17_Chr2g0332281 36.452 310 157 6 215 523 24 294 1.12e-59 206
Msa0567420 MtrunA17_Chr4g0037661 26.128 532 274 13 95 570 49 517 1.90e-46 172
Msa0567420 MtrunA17_Chr1g0163311 24.505 555 294 17 55 541 6 503 9.39e-42 159
Msa0567420 MtrunA17_Chr4g0023801 23.583 547 316 13 97 597 50 540 6.02e-38 148
Msa0567420 MtrunA17_Chr2g0280541 23.210 461 268 14 119 553 1 401 9.23e-27 114
Msa0567420 MtrunA17_Chr1g0206621 37.324 142 78 2 59 194 75 211 4.58e-26 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567420 AT5G10250.2 57.853 624 239 8 22 641 19 622 0.0 697
Msa0567420 AT5G10250.1 56.731 624 231 8 22 641 19 607 0.0 677
Msa0567420 AT5G64330.1 37.551 735 278 14 26 617 24 720 5.56e-157 470
Msa0567420 AT5G64330.2 40.630 603 203 9 44 527 44 610 2.34e-149 447
Msa0567420 AT5G64330.3 40.830 578 187 9 69 527 10 551 4.66e-142 427
Msa0567420 AT5G67385.1 36.640 494 252 8 52 525 25 477 5.82e-108 338
Msa0567420 AT5G48800.1 33.969 524 259 6 34 527 23 489 4.52e-100 318
Msa0567420 AT1G03010.2 34.142 618 295 15 52 606 9 577 6.60e-100 318
Msa0567420 AT5G13600.1 32.492 634 317 18 44 626 18 591 7.80e-100 317
Msa0567420 AT5G67385.4 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567420 AT5G67385.5 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567420 AT5G67385.2 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567420 AT5G67385.3 36.786 473 238 8 73 525 1 432 8.78e-100 316
Msa0567420 AT5G03250.1 33.223 605 308 15 54 610 28 584 1.10e-99 317
Msa0567420 AT1G03010.1 34.237 590 286 12 52 582 36 582 1.36e-99 318
Msa0567420 AT1G03010.3 33.713 614 303 13 52 606 35 603 1.66e-99 317
Msa0567420 AT1G30440.1 33.397 527 271 8 47 527 21 513 2.63e-99 318
Msa0567420 AT2G47860.1 32.823 588 286 11 52 582 37 572 6.26e-97 311
Msa0567420 AT5G67440.3 35.108 507 248 11 46 522 21 476 9.10e-97 308
Msa0567420 AT5G67440.1 35.108 507 248 11 46 522 21 476 9.10e-97 308
Msa0567420 AT3G44820.1 31.376 596 319 13 47 582 18 583 4.74e-95 306
Msa0567420 AT3G49970.1 36.219 439 232 9 60 496 2 394 1.82e-93 298
Msa0567420 AT3G49970.2 36.219 439 232 9 60 496 2 394 1.82e-93 298
Msa0567420 AT1G67900.5 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567420 AT1G67900.1 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567420 AT1G67900.3 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567420 AT1G67900.6 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567420 AT1G67900.2 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567420 AT1G67900.4 32.689 569 278 10 42 551 16 538 1.84e-93 301
Msa0567420 AT2G47860.3 32.455 607 286 13 52 582 37 595 2.71e-93 302
Msa0567420 AT2G14820.2 32.902 541 264 12 38 532 11 498 6.00e-93 300
Msa0567420 AT2G14820.1 32.902 541 264 12 38 532 11 498 6.00e-93 300
Msa0567420 AT3G08570.1 32.743 565 290 12 52 572 34 552 2.24e-92 298
Msa0567420 AT5G67440.2 34.908 487 236 11 66 522 4 439 2.92e-90 290
Msa0567420 AT3G50840.1 30.693 606 348 13 47 630 11 566 1.44e-89 290
Msa0567420 AT2G30520.1 33.673 490 274 9 52 531 30 478 7.32e-89 288
Msa0567420 AT3G08660.1 30.795 591 321 13 52 616 34 562 7.77e-89 288
Msa0567420 AT4G37590.1 31.579 570 281 15 38 563 11 515 9.35e-88 285
Msa0567420 AT5G66560.1 32.212 565 256 12 47 527 14 535 2.96e-87 286
Msa0567420 AT3G26490.1 32.486 551 249 11 43 526 17 511 7.85e-86 280
Msa0567420 AT3G26490.3 32.486 551 249 11 43 526 17 511 7.85e-86 280
Msa0567420 AT3G26490.4 32.486 551 249 11 43 526 17 511 7.85e-86 280
Msa0567420 AT4G31820.1 32.727 495 245 12 46 505 21 462 7.50e-85 277
Msa0567420 AT3G08660.3 30.991 555 297 11 88 616 8 502 1.86e-84 275
Msa0567420 AT5G66560.4 34.810 474 225 10 95 527 66 496 1.89e-84 278
Msa0567420 AT5G66560.3 34.810 474 225 10 95 527 76 506 2.19e-84 278
Msa0567420 AT5G47800.3 32.305 486 274 10 43 505 17 470 3.92e-83 272
Msa0567420 AT5G47800.1 32.305 486 274 10 43 505 17 470 3.92e-83 272
Msa0567420 AT5G47800.4 32.305 486 274 10 43 505 17 470 3.92e-83 272
Msa0567420 AT3G50840.2 31.004 558 313 13 95 630 23 530 8.44e-82 268
Msa0567420 AT3G50840.3 31.004 558 313 13 95 630 23 530 8.44e-82 268
Msa0567420 AT5G47800.2 32.305 486 274 10 43 505 130 583 1.13e-81 271
Msa0567420 AT2G30520.2 33.404 470 262 9 72 531 2 430 2.68e-81 267
Msa0567420 AT2G30520.3 33.404 470 262 9 72 531 2 430 2.68e-81 267
Msa0567420 AT4G31820.2 33.710 442 206 11 99 505 20 409 9.78e-78 257
Msa0567420 AT2G23050.1 30.769 507 262 10 41 526 16 454 1.51e-77 255
Msa0567420 AT2G47860.4 33.705 448 219 8 52 461 37 444 1.09e-74 246
Msa0567420 AT2G47860.6 33.191 467 219 10 52 461 37 467 1.47e-71 239
Msa0567420 AT5G03250.2 31.538 520 261 14 138 610 1 472 2.48e-71 239
Msa0567420 AT2G47860.5 31.474 502 239 10 138 582 1 454 2.10e-68 232
Msa0567420 AT2G47860.2 31.474 502 239 10 138 582 1 454 2.10e-68 232
Msa0567420 AT3G26490.2 31.481 486 221 10 43 461 17 457 2.46e-68 231
Msa0567420 AT3G08660.2 31.494 435 235 8 52 468 34 423 2.17e-67 227
Msa0567420 AT1G52770.1 32.518 409 214 9 197 583 10 378 7.44e-67 226
Msa0567420 AT5G66560.2 30.261 499 232 11 47 461 14 480 3.50e-64 219
Msa0567420 AT3G15570.1 35.000 320 158 7 215 523 29 309 6.31e-58 202
Msa0567420 AT3G19850.1 29.103 457 237 9 94 508 51 462 8.54e-56 199
Msa0567420 AT2G23050.2 29.557 406 201 9 138 526 1 338 8.48e-53 186
Msa0567420 AT5G48130.1 27.083 480 269 10 52 505 39 463 2.36e-52 191
Msa0567420 AT1G50280.2 26.966 445 256 8 95 505 18 427 7.94e-44 164
Msa0567420 AT1G50280.1 26.966 445 256 8 95 505 51 460 8.72e-44 165
Msa0567420 AT3G03510.1 26.840 462 258 14 107 560 8 397 5.13e-37 144
Msa0567420 AT5G17580.2 25.714 490 290 12 42 505 10 451 1.79e-36 144
Msa0567420 AT5G17580.1 26.950 423 245 10 105 505 56 436 9.69e-36 142
Msa0567420 AT3G22104.1 25.664 452 248 12 76 496 26 420 8.92e-35 139
Msa0567420 AT3G22104.4 24.561 399 217 9 125 496 4 345 4.40e-27 115
Msa0567420 AT3G22104.2 24.561 399 217 9 125 496 4 345 4.40e-27 115
Msa0567420 AT3G22104.3 24.561 399 217 9 125 496 4 345 4.40e-27 115

Find 139 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTGTCAGACTGACTTTC+AGG 0.132980 4_1:+68773115 None:intergenic
CACAGCATCTTCTAACAATT+TGG 0.179939 4_1:+68771834 None:intergenic
CTCCAGCCTGATAGATTCTT+TGG 0.216318 4_1:+68769515 None:intergenic
GAGGAGGAATTATGCTTATT+AGG 0.225056 4_1:+68771480 None:intergenic
ATTCAATATTAGTAGGCTTT+TGG 0.241298 4_1:-68770990 Msa0567420:CDS
CATTTGTCGTGCTTTCTTCA+TGG 0.250887 4_1:-68771916 Msa0567420:CDS
CAGCCTCTAATCTCAAATTC+TGG 0.250919 4_1:-68772130 Msa0567420:CDS
TGCGGATAATTTCAGCAATT+AGG 0.253941 4_1:-68771700 Msa0567420:CDS
AACATTGTAATTGAAAGATT+TGG 0.268935 4_1:+68770938 None:intergenic
TTCCCAGAATTTGAGATTAG+AGG 0.277466 4_1:+68772127 None:intergenic
AAGCTGGTGGTTCGATGATT+TGG 0.289085 4_1:-68771747 Msa0567420:CDS
GACTTTCAGGGTCTTGTGCT+TGG 0.289224 4_1:+68773128 None:intergenic
GCGGATAATTTCAGCAATTA+GGG 0.291903 4_1:-68771699 Msa0567420:CDS
TTAGAAGGGCTTAGAGGATA+TGG 0.292438 4_1:-68771596 Msa0567420:CDS
CTCAAACTTGAAAACTTTCC+AGG 0.292504 4_1:-68772100 Msa0567420:CDS
TATCAGGCTGGAGTTTAAAT+TGG 0.321879 4_1:-68769505 Msa0567420:CDS
GCACATTATAAGTGGTTTCC+TGG 0.326824 4_1:+68772812 None:intergenic
TGTCTATAGTTTCAGATGTC+TGG 0.334509 4_1:+68769648 None:intergenic
ACCTGGACAACTGTTCTTAG+AGG 0.338185 4_1:+68770621 None:intergenic
TGCGTTGTATACTTTGGTTC+AGG 0.340379 4_1:+68769761 None:intergenic
ACTTTCAGGGTCTTGTGCTT+GGG 0.351122 4_1:+68773129 None:intergenic
CCTACTAATATTGAATTTCC+CGG 0.364748 4_1:+68770997 None:intergenic
GAGGAGTTAGAAGGGCTTAG+AGG 0.370662 4_1:-68771602 Msa0567420:CDS
ACAATTCTAAAATTCTGTTA+TGG 0.376842 4_1:-68772058 Msa0567420:CDS
TGCAGAGTCTTCTACGGTCT+TGG 0.377991 4_1:+68770709 None:intergenic
TTCTACGGTCTTGGTCAGTT+AGG 0.381517 4_1:+68770718 None:intergenic
CCGGGAAATTCAATATTAGT+AGG 0.395757 4_1:-68770997 Msa0567420:CDS
TTTACCAATGATCTCTGGTT+TGG 0.398294 4_1:+68771667 None:intergenic
GATGCTGCATCCTCACTTGC+GGG 0.402710 4_1:+68771779 None:intergenic
GACAAACCAAAGAATCTATC+AGG 0.413587 4_1:-68769521 Msa0567420:CDS
ACACGGAGAGCCTTCTTCTC+GGG 0.418325 4_1:+68769381 None:intergenic
GAGCTAATTGCTGCAAGCTT+GGG 0.418641 4_1:+68773181 None:intergenic
TCTAACTCCTCATACATTCC+TGG 0.422868 4_1:+68771614 None:intergenic
GACCTTTAGGTAGGTGTCAA+TGG 0.427446 4_1:+68770853 None:intergenic
TTAATTCCTAGATATCAAAA+TGG 0.429080 4_1:-68771320 Msa0567420:CDS
AGCCTCTAATCTCAAATTCT+GGG 0.431924 4_1:-68772129 Msa0567420:CDS
ATGCAACTGCAACAAAAGTC+CGG 0.435207 4_1:-68771016 Msa0567420:CDS
TCTGGTTTCATATATCAGTT+AGG 0.435439 4_1:-68773230 Msa0567420:five_prime_UTR
GACACGGAGAGCCTTCTTCT+CGG 0.443418 4_1:+68769380 None:intergenic
TTATCCGAGTTGAAGTCTAT+AGG 0.445995 4_1:+68772031 None:intergenic
TATCCGAGTTGAAGTCTATA+GGG 0.446756 4_1:+68772032 None:intergenic
AGAACCTGCGTTGTATACTT+TGG 0.448430 4_1:+68769755 None:intergenic
CTTGAATTGAAAAGTCTGTT+GGG 0.449484 4_1:+68772836 None:intergenic
TTCTGTCAGACTGACTTTCA+GGG 0.449988 4_1:+68773116 None:intergenic
TCTACGGTCTTGGTCAGTTA+GGG 0.449995 4_1:+68770719 None:intergenic
ATTAATTTAGACAGCGTGTC+TGG 0.450562 4_1:-68773248 Msa0567420:five_prime_UTR
TCCTTCCATGTAAAACCAAC+AGG 0.451380 4_1:-68769467 Msa0567420:CDS
ATACATTTACCAATGATCTC+TGG 0.452219 4_1:+68771662 None:intergenic
AAGTGGAATCAACTCTCAAC+AGG 0.453660 4_1:-68769678 Msa0567420:CDS
TGAGCTAATTGCTGCAAGCT+TGG 0.454672 4_1:+68773180 None:intergenic
TCTCTAGTTTCACCTCCTGT+TGG 0.456759 4_1:+68769452 None:intergenic
TACCTTGTGCACATTATAAG+TGG 0.465167 4_1:+68772804 None:intergenic
GGAATGTATGAGGAGTTAGA+AGG 0.465676 4_1:-68771611 Msa0567420:CDS
CTTGGTCAGTTAGGGAAGGA+TGG 0.466551 4_1:+68770727 None:intergenic
AGCTATTGTGACATGTTTGT+TGG 0.475232 4_1:+68773060 None:intergenic
TGAAATGATCGAGTATGTTC+TGG 0.476508 4_1:+68770893 None:intergenic
ACTTGAATTGAAAAGTCTGT+TGG 0.476835 4_1:+68772835 None:intergenic
ATTTCAGCAATTAGGGCAAA+GGG 0.478066 4_1:-68771692 Msa0567420:CDS
ATATCAAAATGCGGCTACAT+AGG 0.478687 4_1:-68772166 Msa0567420:CDS
ATCTGCAACAAGAACTTACA+TGG 0.493501 4_1:+68771443 None:intergenic
GCCTCTAAGAACAGTTGTCC+AGG 0.494899 4_1:-68770622 Msa0567420:intron
GCTATTGTGACATGTTTGTT+GGG 0.495372 4_1:+68773061 None:intergenic
ACGAGTGTGGTTTGGTGATG+TGG 0.497567 4_1:+68769415 None:intergenic
GATGCTGTGATTCTATAGCT+TGG 0.503818 4_1:-68771820 Msa0567420:CDS
TGTGAGGAAAGCTTCAGTCT+TGG 0.507277 4_1:+68771937 None:intergenic
GGAACCAAACCAGAGATCAT+TGG 0.508422 4_1:-68771671 Msa0567420:CDS
GATCTACGACGAGTGTGGTT+TGG 0.513023 4_1:+68769407 None:intergenic
CTTGTTGCAGATGCTAAAGA+TGG 0.513993 4_1:-68771432 Msa0567420:CDS
GAAAAGTCTGTTGGGATTCG+AGG 0.514401 4_1:+68772844 None:intergenic
AGCAATGCAAGAGAAGGAAC+CGG 0.515645 4_1:-68769705 Msa0567420:CDS
CATTCCTGGTAGCCATCTCT+TGG 0.516344 4_1:+68771628 None:intergenic
CTGTGATTCTATAGCTTGGA+AGG 0.517789 4_1:-68771816 Msa0567420:CDS
TCAGACCTTGAAAAGAGAGT+TGG 0.522254 4_1:-68771377 Msa0567420:CDS
GTGTAGAATTGTAGTTAAGA+TGG 0.523176 4_1:+68773293 None:intergenic
CAGGCTGGAGTTTAAATTGG+CGG 0.523456 4_1:-68769502 Msa0567420:CDS
TAGAAGGGCTTAGAGGATAT+GGG 0.526409 4_1:-68771595 Msa0567420:CDS
GTAGATCAGCCCCGAGAAGA+AGG 0.527618 4_1:-68769391 Msa0567420:CDS
CTTGTGAAAATCTATCTCCA+TGG 0.528028 4_1:-68771868 Msa0567420:CDS
CCTTGAAAAGAGAGTTGGTA+TGG 0.528846 4_1:-68771372 Msa0567420:CDS
TAGACATAGCAAGGAGCAAA+CGG 0.529211 4_1:-68771516 Msa0567420:CDS
AACCAAAGAATCTATCAGGC+TGG 0.531250 4_1:-68769517 Msa0567420:CDS
AGATATCAAAATGGAGGTCA+AGG 0.533332 4_1:-68771311 Msa0567420:CDS
TGCAACTGCAACAAAAGTCC+GGG 0.536493 4_1:-68771015 Msa0567420:CDS
TTGGTCAGTTAGGGAAGGAT+GGG 0.536654 4_1:+68770728 None:intergenic
AATTTCAGCAATTAGGGCAA+AGG 0.537477 4_1:-68771693 Msa0567420:CDS
AAAAGTCTGTTGGGATTCGA+GGG 0.537749 4_1:+68772845 None:intergenic
AAACATGAAATCACTTCAAG+TGG 0.548709 4_1:-68773152 Msa0567420:exon
AGATGCTGCATCCTCACTTG+CGG 0.555801 4_1:+68771778 None:intergenic
TTGTGAAAATCTATCTCCAT+GGG 0.559597 4_1:-68771867 Msa0567420:CDS
TAAAAGGCTGACCTTTAGGT+AGG 0.562453 4_1:+68770844 None:intergenic
GAGAGCAGCAATGCAAGAGA+AGG 0.563979 4_1:-68769711 Msa0567420:CDS
TATGCCAAGAGATGGCTACC+AGG 0.565928 4_1:-68771632 Msa0567420:CDS
GCAGTAATTCAGCTAAGACT+TGG 0.571771 4_1:+68770915 None:intergenic
ATGACTGAAGAACTAGAAGA+TGG 0.572238 4_1:-68771971 Msa0567420:CDS
GATCTGTTGCAACTTCAGCT+AGG 0.573474 4_1:+68770960 None:intergenic
TAGAATTGTAGTTAAGATGG+TGG 0.574829 4_1:+68773296 None:intergenic
CAGCATCTAATCAAGAAAGC+TGG 0.575400 4_1:-68771763 Msa0567420:CDS
GTTCTGCAGTTCTACCATGT+TGG 0.579053 4_1:+68769589 None:intergenic
GAGTTGATTCCACTTTGTGC+CGG 0.581941 4_1:+68769686 None:intergenic
CACGGAGAGCCTTCTTCTCG+GGG 0.584375 4_1:+68769382 None:intergenic
ATGGCTACCAGGAATGTATG+AGG 0.585069 4_1:-68771621 Msa0567420:CDS
TCAAATGTTTCACATCCACC+TGG 0.585855 4_1:+68772082 None:intergenic
AGCCATTGACACCTACCTAA+AGG 0.587167 4_1:-68770855 Msa0567420:intron
TCATGCAATATGCCAAGAGA+TGG 0.594644 4_1:-68771640 Msa0567420:CDS
CATGGGACAGCATCTTGTTG+AGG 0.601532 4_1:+68771461 None:intergenic
AGAGAGCACTAGACATAGCA+AGG 0.603854 4_1:-68771525 Msa0567420:CDS
CGAGTGTGGTTTGGTGATGT+GGG 0.605082 4_1:+68769416 None:intergenic
GGGCTTAGAGGATATGGGCA+TGG 0.605115 4_1:-68771590 Msa0567420:CDS
GAATGTATGAGGAGTTAGAA+GGG 0.605950 4_1:-68771610 Msa0567420:CDS
CGGTCTTGGTCAGTTAGGGA+AGG 0.612259 4_1:+68770723 None:intergenic
CGAGAATGTAAAGTCCAACA+TGG 0.615865 4_1:-68769603 Msa0567420:CDS
CATAAAATGATCAATGCGGA+GGG 0.617020 4_1:+68771721 None:intergenic
GGGACAGCATCTTGTTGAGG+AGG 0.618679 4_1:+68771464 None:intergenic
GAGAAGGAACCGGCACAAAG+TGG 0.620698 4_1:-68769695 Msa0567420:CDS
ATTCCTAGATATCAAAATGG+AGG 0.624406 4_1:-68771317 Msa0567420:CDS
AAAATGATCAATGCGGAGGG+TGG 0.627548 4_1:+68771724 None:intergenic
CAAAATGCGGCTACATAGGA+CGG 0.632260 4_1:-68772162 Msa0567420:CDS
AGAATTGTAGTTAAGATGGT+GGG 0.638014 4_1:+68773297 None:intergenic
ATGCGGCTACATAGGACGGT+CGG 0.639956 4_1:-68772158 Msa0567420:CDS
GCATAAAATGATCAATGCGG+AGG 0.642568 4_1:+68771720 None:intergenic
AAACTTGAAAACTTTCCAGG+TGG 0.646296 4_1:-68772097 Msa0567420:CDS
ACAGGAGGTGAAACTAGAGA+TGG 0.647018 4_1:-68769449 Msa0567420:CDS
AAGAAAGCACGACAAATGTG+AGG 0.659530 4_1:+68771921 None:intergenic
CATCTAATCAAGAAAGCTGG+TGG 0.660897 4_1:-68771760 Msa0567420:CDS
AACCACTTATAATGTGCACA+AGG 0.667560 4_1:-68772806 Msa0567420:intron
CCTCCCTATAGACTTCAACT+CGG 0.675286 4_1:-68772035 Msa0567420:CDS
GGGCTGATCTACGACGAGTG+TGG 0.675575 4_1:+68769402 None:intergenic
TAATGCTGAGTACTGTTCTG+TGG 0.676472 4_1:+68773267 None:intergenic
CCGAGTTGAAGTCTATAGGG+AGG 0.687521 4_1:+68772035 None:intergenic
CACTATGCAAGACATAGATG+TGG 0.688304 4_1:-68771083 Msa0567420:CDS
TCTGCAACAAGAACTTACAT+GGG 0.692050 4_1:+68771444 None:intergenic
AAAGTCTGTTGGGATTCGAG+GGG 0.692715 4_1:+68772846 None:intergenic
AAGACATAGATGTGGTACAA+CGG 0.695512 4_1:-68771075 Msa0567420:CDS
TTCCATGTAAAACCAACAGG+AGG 0.698959 4_1:-68769464 Msa0567420:CDS
TACCCCTTGATATCAAAATG+CGG 0.703247 4_1:-68772175 Msa0567420:CDS
TGAACCAAAGTATACAACGC+AGG 0.706992 4_1:-68769759 Msa0567420:CDS
TCCGCATAAAATGATCAATG+CGG 0.719094 4_1:+68771717 None:intergenic
TATGGTGTTAGAAGATGCTG+AGG 0.726855 4_1:-68771354 Msa0567420:CDS
CATACTCGATCATTTCACGA+TGG 0.744904 4_1:-68770887 Msa0567420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTATCTTATTAAAAA+TGG + chr4_1:68772751-68772770 None:intergenic 10.0%
!! TTTAACTGCATAAAATATAA+AGG + chr4_1:68769696-68769715 None:intergenic 15.0%
!!! TATTTTCTCTGTTTATTTTT+TGG - chr4_1:68769793-68769812 Msa0567420:intron 15.0%
!!! TTTATATTTTTCCATTTGTA+AGG + chr4_1:68772452-68772471 None:intergenic 15.0%
!!! ATATAAAGGATTCTATTTCT+GGG + chr4_1:68769682-68769701 None:intergenic 20.0%
!! AATATAAAGGATTCTATTTC+TGG + chr4_1:68769683-68769702 None:intergenic 20.0%
!! ACTCATACAAAAATAACTAT+TGG + chr4_1:68769769-68769788 None:intergenic 20.0%
!!! AATGATTTTCTGCATTTATA+AGG + chr4_1:68769925-68769944 None:intergenic 20.0%
!!! TGGTTTTCAAAAAAATTCTA+AGG + chr4_1:68770374-68770393 None:intergenic 20.0%
!! CAACATATAAAAGATTAAGT+TGG + chr4_1:68770471-68770490 None:intergenic 20.0%
!!! CAACTTAATCTTTTATATGT+TGG - chr4_1:68770469-68770488 Msa0567420:intron 20.0%
!!! ACAATTCTAAAATTCTGTTA+TGG - chr4_1:68770627-68770646 Msa0567420:CDS 20.0%
!! TTAATTCCTAGATATCAAAA+TGG - chr4_1:68771365-68771384 Msa0567420:CDS 20.0%
!! AACATTGTAATTGAAAGATT+TGG + chr4_1:68771750-68771769 None:intergenic 20.0%
!! ACAAGAAAATAGGAATTAAA+AGG + chr4_1:68771860-68771879 None:intergenic 20.0%
!!! GATTTCTTTTTCAAAGTTAA+AGG + chr4_1:68772325-68772344 None:intergenic 20.0%
!!! TTTTATTTCTAAATCACAGT+AGG + chr4_1:68772610-68772629 None:intergenic 20.0%
!! TATCTTATTAAAAATGGAGT+TGG + chr4_1:68772745-68772764 None:intergenic 20.0%
! GCATAAAAAATGAACATTCA+AGG - chr4_1:68769652-68769671 Msa0567420:CDS 25.0%
!!! TCTCTGTTTATTTTTTGGTT+TGG - chr4_1:68769798-68769817 Msa0567420:intron 25.0%
!!! CTCTGTTTATTTTTTGGTTT+GGG - chr4_1:68769799-68769818 Msa0567420:intron 25.0%
!!! CCATGTTTTTATTCTAATTC+TGG + chr4_1:68770394-68770413 None:intergenic 25.0%
! CCAGAATTAGAATAAAAACA+TGG - chr4_1:68770391-68770410 Msa0567420:intron 25.0%
!!! ATTTTTTATTTTCGCAGAGT+TGG + chr4_1:68771478-68771497 None:intergenic 25.0%
! CTGCGAAAATAAAAAATAAC+CGG - chr4_1:68771481-68771500 Msa0567420:CDS 25.0%
! AAGATAGAAATTAGTGTCTT+TGG + chr4_1:68771523-68771542 None:intergenic 25.0%
!! ATTCAATATTAGTAGGCTTT+TGG - chr4_1:68771695-68771714 Msa0567420:CDS 25.0%
! TGAATTGAACACAAGAAAAT+AGG + chr4_1:68771870-68771889 None:intergenic 25.0%
!! TATTTTCTTGTGTTCAATTC+AGG - chr4_1:68771869-68771888 Msa0567420:CDS 25.0%
! GGAATTCTTAAAACTCTATT+TGG + chr4_1:68772146-68772165 None:intergenic 25.0%
!!! TGTCATCATTTTTTTCTTCA+TGG + chr4_1:68772167-68772186 None:intergenic 25.0%
! AAAGAAGAAACTTCTTTAGA+AGG - chr4_1:68772388-68772407 Msa0567420:intron 25.0%
AGAATTGTAGTTAAGATGGT+GGG + chr4_1:68769391-68769410 None:intergenic 30.0%
TAGAATTGTAGTTAAGATGG+TGG + chr4_1:68769392-68769411 None:intergenic 30.0%
GTGTAGAATTGTAGTTAAGA+TGG + chr4_1:68769395-68769414 None:intergenic 30.0%
! TCTGGTTTCATATATCAGTT+AGG - chr4_1:68769455-68769474 Msa0567420:CDS 30.0%
AAACATGAAATCACTTCAAG+TGG - chr4_1:68769533-68769552 Msa0567420:CDS 30.0%
CTTGAATTGAAAAGTCTGTT+GGG + chr4_1:68769852-68769871 None:intergenic 30.0%
ACTTGAATTGAAAAGTCTGT+TGG + chr4_1:68769853-68769872 None:intergenic 30.0%
! TTTTACTGAGCTAAACAAGA+AGG + chr4_1:68769950-68769969 None:intergenic 30.0%
TTGTGAAAATCTATCTCCAT+GGG - chr4_1:68770818-68770837 Msa0567420:intron 30.0%
ATACATTTACCAATGATCTC+TGG + chr4_1:68771026-68771045 None:intergenic 30.0%
ATTCCTAGATATCAAAATGG+AGG - chr4_1:68771368-68771387 Msa0567420:CDS 30.0%
!! TCATTTTTCTCCTGTCATAT+TGG - chr4_1:68771425-68771444 Msa0567420:CDS 30.0%
CGAAAATAAAAAATAACCGG+AGG - chr4_1:68771484-68771503 Msa0567420:CDS 30.0%
CCTACTAATATTGAATTTCC+CGG + chr4_1:68771691-68771710 None:intergenic 30.0%
!! TTATTTTGCAGAGTCTTCTA+CGG + chr4_1:68771985-68772004 None:intergenic 30.0%
!! TGTGTTGTTGTGTTTTTCAA+AGG + chr4_1:68772234-68772253 None:intergenic 30.0%
ATTGAAGATCTGAACCAATT+TGG - chr4_1:68772254-68772273 Msa0567420:intron 30.0%
TTGAAGATCTGAACCAATTT+GGG - chr4_1:68772255-68772274 Msa0567420:intron 30.0%
! AAACTTCTTTAGAAGGAAAG+AGG - chr4_1:68772395-68772414 Msa0567420:intron 30.0%
! AACTTCTTTAGAAGGAAAGA+GGG - chr4_1:68772396-68772415 Msa0567420:intron 30.0%
GTACAAATGATCCTTACAAA+TGG - chr4_1:68772438-68772457 Msa0567420:intron 30.0%
! ACTCTATGACACATGATTTT+GGG + chr4_1:68772516-68772535 None:intergenic 30.0%
TGTATAGAGGAAATACTTTG+CGG + chr4_1:68772565-68772584 None:intergenic 30.0%
! ACACAAACTTTTGTGTATAG+AGG + chr4_1:68772578-68772597 None:intergenic 30.0%
AATTCCACAAAACAACATTG+TGG - chr4_1:68772769-68772788 Msa0567420:intron 30.0%
ATTAATTTAGACAGCGTGTC+TGG - chr4_1:68769437-68769456 Msa0567420:CDS 35.0%
GCTATTGTGACATGTTTGTT+GGG + chr4_1:68769627-68769646 None:intergenic 35.0%
AGCTATTGTGACATGTTTGT+TGG + chr4_1:68769628-68769647 None:intergenic 35.0%
! AGACTTTTCAATTCAAGTCC+AGG - chr4_1:68769855-68769874 Msa0567420:intron 35.0%
AACCACTTATAATGTGCACA+AGG - chr4_1:68769879-68769898 Msa0567420:intron 35.0%
TACCTTGTGCACATTATAAG+TGG + chr4_1:68769884-68769903 None:intergenic 35.0%
! AAGAGCACAATCCTGTTAAT+AGG - chr4_1:68770077-68770096 Msa0567420:intron 35.0%
AGAGCACAATCCTGTTAATA+GGG - chr4_1:68770078-68770097 Msa0567420:intron 35.0%
GATTTATGCTCCCTATTAAC+AGG + chr4_1:68770091-68770110 None:intergenic 35.0%
! ACCTCTTTTGGATAGAGAAA+GGG - chr4_1:68770184-68770203 Msa0567420:intron 35.0%
!! TGTTTTGCATGCTTTTAAGG+AGG + chr4_1:68770264-68770283 None:intergenic 35.0%
!!! TGCTGTTTTGCATGCTTTTA+AGG + chr4_1:68770267-68770286 None:intergenic 35.0%
TACCCCTTGATATCAAAATG+CGG - chr4_1:68770510-68770529 Msa0567420:intron 35.0%
!! TAGCCGCATTTTGATATCAA+GGG + chr4_1:68770516-68770535 None:intergenic 35.0%
ATATCAAAATGCGGCTACAT+AGG - chr4_1:68770519-68770538 Msa0567420:intron 35.0%
AGCCTCTAATCTCAAATTCT+GGG - chr4_1:68770556-68770575 Msa0567420:intron 35.0%
! TTCCCAGAATTTGAGATTAG+AGG + chr4_1:68770561-68770580 None:intergenic 35.0%
CTCAAACTTGAAAACTTTCC+AGG - chr4_1:68770585-68770604 Msa0567420:intron 35.0%
AAACTTGAAAACTTTCCAGG+TGG - chr4_1:68770588-68770607 Msa0567420:intron 35.0%
! TATCCGAGTTGAAGTCTATA+GGG + chr4_1:68770656-68770675 None:intergenic 35.0%
! TTATCCGAGTTGAAGTCTAT+AGG + chr4_1:68770657-68770676 None:intergenic 35.0%
ATGACTGAAGAACTAGAAGA+TGG - chr4_1:68770714-68770733 Msa0567420:CDS 35.0%
CTTGTGAAAATCTATCTCCA+TGG - chr4_1:68770817-68770836 Msa0567420:intron 35.0%
! ATTTGGATATTTTCAGCCCA+TGG + chr4_1:68770837-68770856 None:intergenic 35.0%
CACAGCATCTTCTAACAATT+TGG + chr4_1:68770854-68770873 None:intergenic 35.0%
CATAAAATGATCAATGCGGA+GGG + chr4_1:68770967-68770986 None:intergenic 35.0%
!! TCCGCATTGATCATTTTATG+CGG - chr4_1:68770967-68770986 Msa0567420:CDS 35.0%
TCCGCATAAAATGATCAATG+CGG + chr4_1:68770971-68770990 None:intergenic 35.0%
TGCGGATAATTTCAGCAATT+AGG - chr4_1:68770985-68771004 Msa0567420:CDS 35.0%
GCGGATAATTTCAGCAATTA+GGG - chr4_1:68770986-68771005 Msa0567420:CDS 35.0%
AATTTCAGCAATTAGGGCAA+AGG - chr4_1:68770992-68771011 Msa0567420:CDS 35.0%
ATTTCAGCAATTAGGGCAAA+GGG - chr4_1:68770993-68771012 Msa0567420:CDS 35.0%
TTTACCAATGATCTCTGGTT+TGG + chr4_1:68771021-68771040 None:intergenic 35.0%
GAATGTATGAGGAGTTAGAA+GGG - chr4_1:68771075-68771094 Msa0567420:CDS 35.0%
GAGGAGGAATTATGCTTATT+AGG + chr4_1:68771208-68771227 None:intergenic 35.0%
TCTGCAACAAGAACTTACAT+GGG + chr4_1:68771244-68771263 None:intergenic 35.0%
ATCTGCAACAAGAACTTACA+TGG + chr4_1:68771245-68771264 None:intergenic 35.0%
!! TGACCTCCATTTTGATATCT+AGG + chr4_1:68771374-68771393 None:intergenic 35.0%
AGATATCAAAATGGAGGTCA+AGG - chr4_1:68771374-68771393 Msa0567420:CDS 35.0%
AAATGGAGGTCAAGGAAAAA+TGG - chr4_1:68771382-68771401 Msa0567420:CDS 35.0%
TTCTCCTGTCATATTGGAAT+TGG - chr4_1:68771431-68771450 Msa0567420:CDS 35.0%
AATGCCAATTCCAATATGAC+AGG + chr4_1:68771438-68771457 None:intergenic 35.0%
AAGACATAGATGTGGTACAA+CGG - chr4_1:68771610-68771629 Msa0567420:CDS 35.0%
CCGGGAAATTCAATATTAGT+AGG - chr4_1:68771688-68771707 Msa0567420:CDS 35.0%
TGAAATGATCGAGTATGTTC+TGG + chr4_1:68771795-68771814 None:intergenic 35.0%
GAATTAAAAGGCTGACCTTT+AGG + chr4_1:68771848-68771867 None:intergenic 35.0%
!! GATCTTTGGAACTTAGTCTT+CGG - chr4_1:68772098-68772117 Msa0567420:CDS 35.0%
TGAAGATCTGAACCAATTTG+GGG - chr4_1:68772256-68772275 Msa0567420:intron 35.0%
AACCAAACAGCAAGACTAAT+TGG - chr4_1:68772470-68772489 Msa0567420:intron 35.0%
!! TTCCAATTAGTCTTGCTGTT+TGG + chr4_1:68772475-68772494 None:intergenic 35.0%
! CTCTATGACACATGATTTTG+GGG + chr4_1:68772515-68772534 None:intergenic 35.0%
! CACTCTATGACACATGATTT+TGG + chr4_1:68772517-68772536 None:intergenic 35.0%
ATCATGTGTCATAGAGTGAA+AGG - chr4_1:68772519-68772538 Msa0567420:intron 35.0%
TCATGTGTCATAGAGTGAAA+GGG - chr4_1:68772520-68772539 Msa0567420:intron 35.0%
!!! AGATCCACAATGTTGTTTTG+TGG + chr4_1:68772776-68772795 None:intergenic 35.0%
ACAAAACAACATTGTGGATC+TGG - chr4_1:68772775-68772794 Msa0567420:intron 35.0%
!! TGGACATATTAGCTCTGAAA+AGG + chr4_1:68772876-68772895 None:intergenic 35.0%
CAGAGCTAATATGTCCATAA+TGG - chr4_1:68772879-68772898 Msa0567420:intron 35.0%
TCTAAGCTGAATCACCATTA+TGG + chr4_1:68772896-68772915 None:intergenic 35.0%
TGTCTATAGTTTCAGATGTC+TGG + chr4_1:68773040-68773059 None:intergenic 35.0%
GACAAACCAAAGAATCTATC+AGG - chr4_1:68773164-68773183 Msa0567420:exon 35.0%
TATCAGGCTGGAGTTTAAAT+TGG - chr4_1:68773180-68773199 Msa0567420:five_prime_UTR 35.0%
! TAATGCTGAGTACTGTTCTG+TGG + chr4_1:68769421-68769440 None:intergenic 40.0%
! TTCTGTCAGACTGACTTTCA+GGG + chr4_1:68769572-68769591 None:intergenic 40.0%
!!! TTTTTGGTTTGGGCTTATGC+AGG - chr4_1:68769809-68769828 Msa0567420:intron 40.0%
AAAAGTCTGTTGGGATTCGA+GGG + chr4_1:68769843-68769862 None:intergenic 40.0%
GCACATTATAAGTGGTTTCC+TGG + chr4_1:68769876-68769895 None:intergenic 40.0%
TTCCAAACAGTATCATCAGC+AGG - chr4_1:68769973-68769992 Msa0567420:intron 40.0%
TCCAAACAGTATCATCAGCA+GGG - chr4_1:68769974-68769993 Msa0567420:intron 40.0%
TATCATCAGCAGGGTATATG+AGG - chr4_1:68769983-68770002 Msa0567420:intron 40.0%
ATCATCAGCAGGGTATATGA+GGG - chr4_1:68769984-68770003 Msa0567420:intron 40.0%
CACAAACACACTAGGAAGAA+AGG + chr4_1:68770121-68770140 None:intergenic 40.0%
CTTTCTCTATCCAAAAGAGG+TGG + chr4_1:68770185-68770204 None:intergenic 40.0%
! CACCTCTTTTGGATAGAGAA+AGG - chr4_1:68770183-68770202 Msa0567420:intron 40.0%
! CCTCTTTTGGATAGAGAAAG+GGG - chr4_1:68770185-68770204 Msa0567420:intron 40.0%
! CTCTTTTGGATAGAGAAAGG+GGG - chr4_1:68770186-68770205 Msa0567420:intron 40.0%
TAGACAGAGAACATCAGACA+TGG - chr4_1:68770229-68770248 Msa0567420:intron 40.0%
AGAGAACATCAGACATGGAT+AGG - chr4_1:68770234-68770253 Msa0567420:intron 40.0%
GAGAACATCAGACATGGATA+GGG - chr4_1:68770235-68770254 Msa0567420:intron 40.0%
!! AGCCGCATTTTGATATCAAG+GGG + chr4_1:68770515-68770534 None:intergenic 40.0%
!! GTAGCCGCATTTTGATATCA+AGG + chr4_1:68770517-68770536 None:intergenic 40.0%
CAGCCTCTAATCTCAAATTC+TGG - chr4_1:68770555-68770574 Msa0567420:intron 40.0%
TCAAATGTTTCACATCCACC+TGG + chr4_1:68770606-68770625 None:intergenic 40.0%
! AAGAAAGCACGACAAATGTG+AGG + chr4_1:68770767-68770786 None:intergenic 40.0%
CATTTGTCGTGCTTTCTTCA+TGG - chr4_1:68770769-68770788 Msa0567420:intron 40.0%
GATGCTGTGATTCTATAGCT+TGG - chr4_1:68770865-68770884 Msa0567420:CDS 40.0%
CTGTGATTCTATAGCTTGGA+AGG - chr4_1:68770869-68770888 Msa0567420:CDS 40.0%
CAGCATCTAATCAAGAAAGC+TGG - chr4_1:68770922-68770941 Msa0567420:CDS 40.0%
CATCTAATCAAGAAAGCTGG+TGG - chr4_1:68770925-68770944 Msa0567420:CDS 40.0%
GCATAAAATGATCAATGCGG+AGG + chr4_1:68770968-68770987 None:intergenic 40.0%
TCATGCAATATGCCAAGAGA+TGG - chr4_1:68771045-68771064 Msa0567420:CDS 40.0%
TCTAACTCCTCATACATTCC+TGG + chr4_1:68771074-68771093 None:intergenic 40.0%
GGAATGTATGAGGAGTTAGA+AGG - chr4_1:68771074-68771093 Msa0567420:CDS 40.0%
TTAGAAGGGCTTAGAGGATA+TGG - chr4_1:68771089-68771108 Msa0567420:CDS 40.0%
TAGAAGGGCTTAGAGGATAT+GGG - chr4_1:68771090-68771109 Msa0567420:CDS 40.0%
TAGACATAGCAAGGAGCAAA+CGG - chr4_1:68771169-68771188 Msa0567420:intron 40.0%
CTTGTTGCAGATGCTAAAGA+TGG - chr4_1:68771253-68771272 Msa0567420:intron 40.0%
TCAGACCTTGAAAAGAGAGT+TGG - chr4_1:68771308-68771327 Msa0567420:CDS 40.0%
! CCATACCAACTCTCTTTTCA+AGG + chr4_1:68771316-68771335 None:intergenic 40.0%
!! CCTTGAAAAGAGAGTTGGTA+TGG - chr4_1:68771313-68771332 Msa0567420:CDS 40.0%
! TATGGTGTTAGAAGATGCTG+AGG - chr4_1:68771331-68771350 Msa0567420:CDS 40.0%
GGAATTGGCATTAATGCTGA+AGG - chr4_1:68771446-68771465 Msa0567420:CDS 40.0%
!! TGGTCATTTTCATAAGCCTC+CGG + chr4_1:68771503-68771522 None:intergenic 40.0%
! TCTTGCATAGTGCAGTTTTC+GGG + chr4_1:68771594-68771613 None:intergenic 40.0%
! GTCTTGCATAGTGCAGTTTT+CGG + chr4_1:68771595-68771614 None:intergenic 40.0%
CACTATGCAAGACATAGATG+TGG - chr4_1:68771602-68771621 Msa0567420:CDS 40.0%
ATGCAACTGCAACAAAAGTC+CGG - chr4_1:68771669-68771688 Msa0567420:CDS 40.0%
GCAGTAATTCAGCTAAGACT+TGG + chr4_1:68771773-68771792 None:intergenic 40.0%
CATACTCGATCATTTCACGA+TGG - chr4_1:68771798-68771817 Msa0567420:CDS 40.0%
TAAAAGGCTGACCTTTAGGT+AGG + chr4_1:68771844-68771863 None:intergenic 40.0%
GACTAAGTTCCAAAGATCCA+GGG + chr4_1:68772096-68772115 None:intergenic 40.0%
AGACTAAGTTCCAAAGATCC+AGG + chr4_1:68772097-68772116 None:intergenic 40.0%
GGTATTTAAGCAGATGCTAG+TGG + chr4_1:68772213-68772232 None:intergenic 40.0%
GAAAACATCTGACCCCAAAT+TGG + chr4_1:68772271-68772290 None:intergenic 40.0%
! AGTTTTCCATCTGCATGGAA+GGG + chr4_1:68772347-68772366 None:intergenic 40.0%
! TAGTTTTCCATCTGCATGGA+AGG + chr4_1:68772348-68772367 None:intergenic 40.0%
!!! TCACTAGTTTTCCATCTGCA+TGG + chr4_1:68772352-68772371 None:intergenic 40.0%
!! ATGACACATGATTTTGGGGA+GGG + chr4_1:68772511-68772530 None:intergenic 40.0%
!! TATGACACATGATTTTGGGG+AGG + chr4_1:68772512-68772531 None:intergenic 40.0%
! GTGGATCTGGTTCACATTAT+AGG - chr4_1:68772788-68772807 Msa0567420:intron 40.0%
! TCTGGTTCACATTATAGGCA+TGG - chr4_1:68772793-68772812 Msa0567420:intron 40.0%
! TGCGTTGTATACTTTGGTTC+AGG + chr4_1:68772927-68772946 None:intergenic 40.0%
TGAACCAAAGTATACAACGC+AGG - chr4_1:68772926-68772945 Msa0567420:intron 40.0%
AGAACCTGCGTTGTATACTT+TGG + chr4_1:68772933-68772952 None:intergenic 40.0%
AAGTGGAATCAACTCTCAAC+AGG - chr4_1:68773007-68773026 Msa0567420:intron 40.0%
CGAGAATGTAAAGTCCAACA+TGG - chr4_1:68773082-68773101 Msa0567420:CDS 40.0%
AACCAAAGAATCTATCAGGC+TGG - chr4_1:68773168-68773187 Msa0567420:exon 40.0%
TCCTTCCATGTAAAACCAAC+AGG - chr4_1:68773218-68773237 Msa0567420:five_prime_UTR 40.0%
!!! TCCTGTTGGTTTTACATGGA+AGG + chr4_1:68773222-68773241 None:intergenic 40.0%
TTCCATGTAAAACCAACAGG+AGG - chr4_1:68773221-68773240 Msa0567420:five_prime_UTR 40.0%
GAGCTAATTGCTGCAAGCTT+GGG + chr4_1:68769507-68769526 None:intergenic 45.0%
TGAGCTAATTGCTGCAAGCT+TGG + chr4_1:68769508-68769527 None:intergenic 45.0%
! ACTTTCAGGGTCTTGTGCTT+GGG + chr4_1:68769559-68769578 None:intergenic 45.0%
! GTTCTGTCAGACTGACTTTC+AGG + chr4_1:68769573-68769592 None:intergenic 45.0%
AAAGTCTGTTGGGATTCGAG+GGG + chr4_1:68769842-68769861 None:intergenic 45.0%
GAAAAGTCTGTTGGGATTCG+AGG + chr4_1:68769844-68769863 None:intergenic 45.0%
ACCCTGCTGATGATACTGTT+TGG + chr4_1:68769978-68769997 None:intergenic 45.0%
CACACACACACAAACACACT+AGG + chr4_1:68770129-68770148 None:intergenic 45.0%
TCTATCCAAAAGAGGTGGTG+GGG + chr4_1:68770180-68770199 None:intergenic 45.0%
CTCTATCCAAAAGAGGTGGT+GGG + chr4_1:68770181-68770200 None:intergenic 45.0%
TCTCTATCCAAAAGAGGTGG+TGG + chr4_1:68770182-68770201 None:intergenic 45.0%
CCCCTTTCTCTATCCAAAAG+AGG + chr4_1:68770188-68770207 None:intergenic 45.0%
GGGAAAATTTCCCTCAAGAG+AGG - chr4_1:68770206-68770225 Msa0567420:intron 45.0%
TCTCTGTCTACCTCTCTTGA+GGG + chr4_1:68770219-68770238 None:intergenic 45.0%
TTCTCTGTCTACCTCTCTTG+AGG + chr4_1:68770220-68770239 None:intergenic 45.0%
CAAAATGCGGCTACATAGGA+CGG - chr4_1:68770523-68770542 Msa0567420:intron 45.0%
CCTCCCTATAGACTTCAACT+CGG - chr4_1:68770650-68770669 Msa0567420:CDS 45.0%
TGTGAGGAAAGCTTCAGTCT+TGG + chr4_1:68770751-68770770 None:intergenic 45.0%
TAAAAATGAGCCCGCAAGTG+AGG - chr4_1:68770896-68770915 Msa0567420:CDS 45.0%
AAGCTGGTGGTTCGATGATT+TGG - chr4_1:68770938-68770957 Msa0567420:CDS 45.0%
AAAATGATCAATGCGGAGGG+TGG + chr4_1:68770964-68770983 None:intergenic 45.0%
GGAACCAAACCAGAGATCAT+TGG - chr4_1:68771014-68771033 Msa0567420:CDS 45.0%
ATGGCTACCAGGAATGTATG+AGG - chr4_1:68771064-68771083 Msa0567420:CDS 45.0%
!! AGAGAGCACTAGACATAGCA+AGG - chr4_1:68771160-68771179 Msa0567420:intron 45.0%
!!! GTGCAGTTTTCGGGTGAATT+AGG + chr4_1:68771585-68771604 None:intergenic 45.0%
TGCAACTGCAACAAAAGTCC+GGG - chr4_1:68771670-68771689 Msa0567420:CDS 45.0%
GATCTGTTGCAACTTCAGCT+AGG + chr4_1:68771728-68771747 None:intergenic 45.0%
AGCCATTGACACCTACCTAA+AGG - chr4_1:68771830-68771849 Msa0567420:CDS 45.0%
GACCTTTAGGTAGGTGTCAA+TGG + chr4_1:68771835-68771854 None:intergenic 45.0%
TTGGTCAGTTAGGGAAGGAT+GGG + chr4_1:68771960-68771979 None:intergenic 45.0%
TCTACGGTCTTGGTCAGTTA+GGG + chr4_1:68771969-68771988 None:intergenic 45.0%
TTCTACGGTCTTGGTCAGTT+AGG + chr4_1:68771970-68771989 None:intergenic 45.0%
ACCTGGACAACTGTTCTTAG+AGG + chr4_1:68772067-68772086 None:intergenic 45.0%
AAGATCCAGGGAACTTTACC+TGG + chr4_1:68772084-68772103 None:intergenic 45.0%
GGTAAAGTTCCCTGGATCTT+TGG - chr4_1:68772084-68772103 Msa0567420:CDS 45.0%
AGAAATCCCTTCCATGCAGA+TGG - chr4_1:68772338-68772357 Msa0567420:intron 45.0%
! GCATGGCATCTAAGATAGTG+TGG - chr4_1:68772810-68772829 Msa0567420:CDS 45.0%
AGCAATGCAAGAGAAGGAAC+CGG - chr4_1:68772980-68772999 Msa0567420:intron 45.0%
! GAGTTGATTCCACTTTGTGC+CGG + chr4_1:68773002-68773021 None:intergenic 45.0%
GTTCTGCAGTTCTACCATGT+TGG + chr4_1:68773099-68773118 None:intergenic 45.0%
CTCCAGCCTGATAGATTCTT+TGG + chr4_1:68773173-68773192 None:intergenic 45.0%
CAGGCTGGAGTTTAAATTGG+CGG - chr4_1:68773183-68773202 Msa0567420:five_prime_UTR 45.0%
!!! CACCTCCTGTTGGTTTTACA+TGG + chr4_1:68773226-68773245 None:intergenic 45.0%
! TCTCTAGTTTCACCTCCTGT+TGG + chr4_1:68773236-68773255 None:intergenic 45.0%
ACAGGAGGTGAAACTAGAGA+TGG - chr4_1:68773236-68773255 Msa0567420:five_prime_UTR 45.0%
! GACTTTCAGGGTCTTGTGCT+TGG + chr4_1:68769560-68769579 None:intergenic 50.0%
! CCGAGTTGAAGTCTATAGGG+AGG + chr4_1:68770653-68770672 None:intergenic 50.0%
AGATGCTGCATCCTCACTTG+CGG + chr4_1:68770910-68770929 None:intergenic 50.0%
TATGCCAAGAGATGGCTACC+AGG - chr4_1:68771053-68771072 Msa0567420:CDS 50.0%
CATTCCTGGTAGCCATCTCT+TGG + chr4_1:68771060-68771079 None:intergenic 50.0%
GAGGAGTTAGAAGGGCTTAG+AGG - chr4_1:68771083-68771102 Msa0567420:CDS 50.0%
CATGGGACAGCATCTTGTTG+AGG + chr4_1:68771227-68771246 None:intergenic 50.0%
CTTGGTCAGTTAGGGAAGGA+TGG + chr4_1:68771961-68771980 None:intergenic 50.0%
TGCAGAGTCTTCTACGGTCT+TGG + chr4_1:68771979-68771998 None:intergenic 50.0%
! GCCTCTAAGAACAGTTGTCC+AGG - chr4_1:68772063-68772082 Msa0567420:CDS 50.0%
GTTGTCCAGGTAAAGTTCCC+TGG - chr4_1:68772076-68772095 Msa0567420:CDS 50.0%
GAGAGCAGCAATGCAAGAGA+AGG - chr4_1:68772974-68772993 Msa0567420:intron 50.0%
!! CGAGTGTGGTTTGGTGATGT+GGG + chr4_1:68773272-68773291 None:intergenic 50.0%
!! ACGAGTGTGGTTTGGTGATG+TGG + chr4_1:68773273-68773292 None:intergenic 50.0%
GATCTACGACGAGTGTGGTT+TGG + chr4_1:68773281-68773300 None:intergenic 50.0%
!! TCCAAAAGAGGTGGTGGGGT+GGG + chr4_1:68770176-68770195 None:intergenic 55.0%
!! ATCCAAAAGAGGTGGTGGGG+TGG + chr4_1:68770177-68770196 None:intergenic 55.0%
ATGCGGCTACATAGGACGGT+CGG - chr4_1:68770527-68770546 Msa0567420:intron 55.0%
GATGCTGCATCCTCACTTGC+GGG + chr4_1:68770909-68770928 None:intergenic 55.0%
GGGCTTAGAGGATATGGGCA+TGG - chr4_1:68771095-68771114 Msa0567420:CDS 55.0%
GGGACAGCATCTTGTTGAGG+AGG + chr4_1:68771224-68771243 None:intergenic 55.0%
CGGTCTTGGTCAGTTAGGGA+AGG + chr4_1:68771965-68771984 None:intergenic 55.0%
GAGAAGGAACCGGCACAAAG+TGG - chr4_1:68772990-68773009 Msa0567420:intron 55.0%
GTAGATCAGCCCCGAGAAGA+AGG - chr4_1:68773294-68773313 Msa0567420:five_prime_UTR 55.0%
ACACGGAGAGCCTTCTTCTC+GGG + chr4_1:68773307-68773326 None:intergenic 55.0%
GACACGGAGAGCCTTCTTCT+CGG + chr4_1:68773308-68773327 None:intergenic 55.0%
! TCCCACCCCACCACCTCTTT+TGG - chr4_1:68770172-68770191 Msa0567420:intron 60.0%
GGGCTGATCTACGACGAGTG+TGG + chr4_1:68773286-68773305 None:intergenic 60.0%
CACGGAGAGCCTTCTTCTCG+GGG + chr4_1:68773306-68773325 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 68769376 68773331 68769376 ID=Msa0567420;Name=Msa0567420
chr4_1 mRNA 68769376 68773331 68769376 ID=Msa0567420-mRNA-1;Parent=Msa0567420;Name=Msa0567420-mRNA-1;_AED=0.08;_eAED=0.08;_QI=161|0.8|0.83|1|1|1|6|0|641
chr4_1 exon 68769376 68769792 68769376 ID=Msa0567420-mRNA-1:exon:22772;Parent=Msa0567420-mRNA-1
chr4_1 exon 68770623 68770751 68770623 ID=Msa0567420-mRNA-1:exon:22771;Parent=Msa0567420-mRNA-1
chr4_1 exon 68770856 68771129 68770856 ID=Msa0567420-mRNA-1:exon:22770;Parent=Msa0567420-mRNA-1
chr4_1 exon 68771299 68772197 68771299 ID=Msa0567420-mRNA-1:exon:22769;Parent=Msa0567420-mRNA-1
chr4_1 exon 68772807 68772876 68772807 ID=Msa0567420-mRNA-1:exon:22768;Parent=Msa0567420-mRNA-1
chr4_1 exon 68773034 68773331 68773034 ID=Msa0567420-mRNA-1:exon:22767;Parent=Msa0567420-mRNA-1
chr4_1 five_prime_UTR 68773171 68773331 68773171 ID=Msa0567420-mRNA-1:five_prime_utr;Parent=Msa0567420-mRNA-1
chr4_1 CDS 68773034 68773170 68773034 ID=Msa0567420-mRNA-1:cds;Parent=Msa0567420-mRNA-1
chr4_1 CDS 68772807 68772876 68772807 ID=Msa0567420-mRNA-1:cds;Parent=Msa0567420-mRNA-1
chr4_1 CDS 68771299 68772197 68771299 ID=Msa0567420-mRNA-1:cds;Parent=Msa0567420-mRNA-1
chr4_1 CDS 68770856 68771129 68770856 ID=Msa0567420-mRNA-1:cds;Parent=Msa0567420-mRNA-1
chr4_1 CDS 68770623 68770751 68770623 ID=Msa0567420-mRNA-1:cds;Parent=Msa0567420-mRNA-1
chr4_1 CDS 68769376 68769792 68769376 ID=Msa0567420-mRNA-1:cds;Parent=Msa0567420-mRNA-1
Gene Sequence

>Msa0567420

ATGAAATCACTTCAAGTGGCCCAAGCACAAGACCCTGAAAGTCAGTCTGACAGAACTGAACAAGTTTCAGATAAAAGCATAGTTATTCCCAACAAACATGTCACAATAGCTGATAGCATAAAAAATGAACATTCAAGGTTCGTTGCCCCTCGAATCCCAACAGACTTTTCAATTCAAGTCCAGGAAACCACTTATAATGTGCACAAGTACCCCTTGATATCAAAATGCGGCTACATAGGACGGTCGGAAGTACAGCCTCTAATCTCAAATTCTGGGAATGTGCTCAAACTTGAAAACTTTCCAGGTGGATGTGAAACATTTGAAACAATTCTAAAATTCTGTTATGGCCTCCCTATAGACTTCAACTCGGATAATGTAGCTGCACTAAGATGTGCATCAGAATTTCTAGAGATGACTGAAGAACTAGAAGATGGCAATCTCATTTCCAAGACTGAAGCTTTCCTCACATTTGTCGTGCTTTCTTCATGGAAAGACACTGTTACTGTTCTGAAATCTTGTGAAAATCTATCTCCATGGGCTGAAAATATCCAAATTGTTAGAAGATGCTGTGATTCTATAGCTTGGAAGGCTACTAAAAATGAGCCCGCAAGTGAGGATGCAGCATCTAATCAAGAAAGCTGGTGGTTCGATGATTTGGCCACCCTCCGCATTGATCATTTTATGCGGATAATTTCAGCAATTAGGGCAAAGGGAACCAAACCAGAGATCATTGGTAAATGTATCATGCAATATGCCAAGAGATGGCTACCAGGAATGTATGAGGAGTTAGAAGGGCTTAGAGGATATGGGCATGGAAAAAGTAATCTACAGTTCACTATATTTAGCATGAAGAAAGAAGAGAGCACTAGACATAGCAAGGAGCAAACGGCTATTATTGAAAGCCTAATAAGCATAATTCCTCCTCAACAAGATGCTGTCCCATGTAAGTTCTTGTTGCAGATGCTAAAGATGGCAATGATGTATTCTGTATCATCAGCTCTCATTTCAGACCTTGAAAAGAGAGTTGGTATGGTGTTAGAAGATGCTGAGGTGAATGATCTCTTAATTCCTAGATATCAAAATGGAGGTCAAGGAAAAATGGTCAACATGCCTAATTCACCCGAAAACTGCACTATGCAAGACATAGATGTGGTACAACGGATTGTTGAATACTTCTTGATGCATGAAAAACAGCAAATGCAACTGCAACAAAAGTCCGGGAAATTCAATATTAGTAGGCTTTTGGACAATTACCTAGCTGAAGTTGCAACAGATCCAAATCTTTCAATTACAATGTTCCAAGTCTTAGCTGAATTACTGCCAGAACATACTCGATCATTTCACGATGGTCTATATAGAGCCATTGACACCTACCTAAAGACCCATCCTTCCCTAACTGACCAAGACCGTAGAAGACTCTGCAAAATAATGAACTGTGAAAAACTCTCACTTGATGCATGCATACATGCTGCACAAAATGATAGATTGCCTCTAAGAACAGTTGTCCAGAAATTGTCACCTGAACCAAAGTATACAACGCAGGTTCTATTTGCAGAGCAAGTAAAGATGAGAGCAGCAATGCAAGAGAAGGAACCGGCACAAAGTGGAATCAACTCTCAACAGGATGTAAACCAGACATCTGAAACTATAGACATAAAAACACTCAAAGCAGAACTCGAGAATGTAAAGTCCAACATGGTAGAACTGCAGAACGACTACTTTGAGTTGCAAGAAGAGTATAAAAAGTTAACTAGCAACGACAAACCAAAGAATCTATCAGGCTGGAGTTTAAATTGGCGGAAGATCAAGAAATCCTTCCATGTAAAACCAACAGGAGGTGAAACTAGAGATGGACAAGATATGCCCACATCACCAAACCACACTCGTCGTAGATCAGCCCCGAGAAGAAGGCTCTCCGTGTCTTAA
Protein sequence

>Msa0567420

MKSLQVAQAQDPESQSDRTEQVSDKSIVIPNKHVTIADSIKNEHSRFVAPRIPTDFSIQVQETTYNVHKYPLISKCGYIGRSEVQPLISNSGNVLKLENFPGGCETFETILKFCYGLPIDFNSDNVAALRCASEFLEMTEELEDGNLISKTEAFLTFVVLSSWKDTVTVLKSCENLSPWAENIQIVRRCCDSIAWKATKNEPASEDAASNQESWWFDDLATLRIDHFMRIISAIRAKGTKPEIIGKCIMQYAKRWLPGMYEELEGLRGYGHGKSNLQFTIFSMKKEESTRHSKEQTAIIESLISIIPPQQDAVPCKFLLQMLKMAMMYSVSSALISDLEKRVGMVLEDAEVNDLLIPRYQNGGQGKMVNMPNSPENCTMQDIDVVQRIVEYFLMHEKQQMQLQQKSGKFNISRLLDNYLAEVATDPNLSITMFQVLAELLPEHTRSFHDGLYRAIDTYLKTHPSLTDQDRRRLCKIMNCEKLSLDACIHAAQNDRLPLRTVVQKLSPEPKYTTQVLFAEQVKMRAAMQEKEPAQSGINSQQDVNQTSETIDIKTLKAELENVKSNMVELQNDYFELQEEYKKLTSNDKPKNLSGWSLNWRKIKKSFHVKPTGGETRDGQDMPTSPNHTRRRSAPRRRLSVS*