AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0567490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567490 MtrunA17_Chr8g0382231 99.185 368 3 0 1 368 1 368 0.0 764
Msa0567490 MtrunA17_Chr4g0050021 44.591 379 160 7 27 360 18 391 1.17e-105 325
Msa0567490 MtrunA17_Chr2g0279481 43.250 400 173 7 13 362 3 398 2.09e-104 322
Msa0567490 MtrunA17_Chr1g0191711 37.982 337 184 6 24 356 29 344 1.31e-77 248
Msa0567490 MtrunA17_Chr5g0401451 33.880 366 195 7 19 363 10 349 3.22e-65 218
Msa0567490 MtrunA17_Chr2g0303541 33.429 350 197 5 37 359 10 350 3.01e-63 213
Msa0567490 MtrunA17_Chr8g0367891 35.783 313 168 4 55 356 29 319 1.68e-61 207
Msa0567490 MtrunA17_Chr4g0053841 35.843 332 166 7 52 359 13 321 3.39e-61 206
Msa0567490 MtrunA17_Chr1g0181181 33.229 319 200 5 41 357 15 322 2.89e-60 203
Msa0567490 MtrunA17_Chr2g0320691 31.105 344 203 5 44 361 15 350 1.80e-59 202
Msa0567490 MtrunA17_Chr4g0010091 31.198 359 215 5 20 363 2 343 5.69e-59 201
Msa0567490 MtrunA17_Chr8g0374011 34.167 360 174 8 47 359 15 358 1.05e-58 201
Msa0567490 MtrunA17_Chr4g0076521 33.433 335 193 6 45 365 19 337 1.13e-56 194
Msa0567490 MtrunA17_Chr1g0150191 31.412 347 180 8 46 362 21 339 2.74e-56 194
Msa0567490 MtrunA17_Chr2g0285511 33.735 332 179 7 52 357 26 342 8.87e-56 192
Msa0567490 MtrunA17_Chr1g0195911 33.033 333 183 7 52 368 36 344 5.74e-55 190
Msa0567490 MtrunA17_Chr3g0108331 34.169 319 181 7 52 364 24 319 6.54e-55 189
Msa0567490 MtrunA17_Chr2g0290741 33.038 339 187 8 35 354 8 325 3.13e-54 187
Msa0567490 MtrunA17_Chr5g0432861 34.286 315 194 4 52 364 25 328 5.34e-54 187
Msa0567490 MtrunA17_Chr1g0207461 32.849 344 173 11 42 356 18 332 1.79e-53 186
Msa0567490 MtrunA17_Chr7g0245201 33.731 335 194 6 42 362 51 371 2.42e-53 186
Msa0567490 MtrunA17_Chr7g0268781 33.333 327 170 7 52 357 36 335 2.94e-52 183
Msa0567490 MtrunA17_Chr5g0401341 30.946 349 183 8 38 356 11 331 5.57e-52 182
Msa0567490 MtrunA17_Chr5g0412591 32.353 340 168 7 42 357 16 317 1.00e-49 176
Msa0567490 MtrunA17_Chr1g0199631 31.410 312 171 5 70 357 8 300 6.58e-48 171
Msa0567490 MtrunA17_Chr1g0206621 37.324 142 78 2 59 194 75 211 4.43e-27 107
Msa0567490 MtrunA17_Chr4g0003891 32.653 147 85 1 215 361 46 178 3.56e-24 103
Msa0567490 MtrunA17_Chr2g0332281 34.014 147 85 2 215 361 24 158 5.16e-21 94.0
Msa0567490 MtrunA17_Chr4g0037661 23.585 318 161 7 95 362 49 334 4.41e-20 91.7
Msa0567490 MtrunA17_Chr1g0163311 22.928 362 188 13 55 362 6 330 1.07e-16 81.3
Msa0567490 MtrunA17_Chr4g0023801 21.875 288 166 7 97 357 50 305 2.50e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0567490 AT5G10250.1 58.286 350 144 2 22 369 19 368 3.54e-145 424
Msa0567490 AT5G10250.2 58.453 349 143 2 22 368 19 367 2.03e-144 423
Msa0567490 AT5G64330.2 39.416 411 147 6 44 364 44 442 3.02e-93 293
Msa0567490 AT5G64330.1 39.416 411 147 6 44 364 44 442 9.80e-93 293
Msa0567490 AT5G64330.3 40.155 386 129 6 69 364 10 383 2.34e-88 278
Msa0567490 AT5G67385.1 35.045 331 182 5 52 367 25 337 1.06e-63 214
Msa0567490 AT3G49970.1 35.922 309 174 5 60 368 2 286 1.53e-63 211
Msa0567490 AT3G49970.2 35.922 309 174 5 60 368 2 286 1.53e-63 211
Msa0567490 AT5G67440.3 33.626 342 178 7 46 364 21 336 5.81e-61 206
Msa0567490 AT5G67440.1 33.626 342 178 7 46 364 21 336 5.81e-61 206
Msa0567490 AT5G48800.1 32.759 348 183 3 34 363 23 337 6.79e-60 204
Msa0567490 AT5G03250.1 34.328 335 173 6 54 357 28 346 1.08e-58 200
Msa0567490 AT1G30440.1 30.814 344 199 5 47 361 21 354 3.15e-58 200
Msa0567490 AT2G30520.1 33.866 313 192 5 52 364 30 327 1.86e-56 194
Msa0567490 AT2G47860.4 33.750 320 174 5 52 357 37 332 2.89e-56 190
Msa0567490 AT5G67385.4 35.161 310 168 5 73 367 1 292 1.01e-55 191
Msa0567490 AT5G67385.5 35.161 310 168 5 73 367 1 292 1.01e-55 191
Msa0567490 AT5G67385.2 35.161 310 168 5 73 367 1 292 1.01e-55 191
Msa0567490 AT5G67385.3 35.161 310 168 5 73 367 1 292 1.01e-55 191
Msa0567490 AT2G47860.1 33.750 320 174 5 52 357 37 332 1.18e-55 193
Msa0567490 AT1G67900.5 34.311 341 177 6 42 357 16 334 3.35e-55 191
Msa0567490 AT1G67900.1 34.311 341 177 6 42 357 16 334 3.35e-55 191
Msa0567490 AT1G67900.3 34.311 341 177 6 42 357 16 334 3.35e-55 191
Msa0567490 AT1G67900.6 34.311 341 177 6 42 357 16 334 3.35e-55 191
Msa0567490 AT1G67900.2 34.311 341 177 6 42 357 16 334 3.35e-55 191
Msa0567490 AT1G67900.4 34.311 341 177 6 42 357 16 334 3.35e-55 191
Msa0567490 AT3G26490.2 33.427 356 171 7 43 363 17 341 5.42e-55 188
Msa0567490 AT5G67440.2 33.230 322 166 7 66 364 4 299 1.06e-54 188
Msa0567490 AT3G26490.1 33.427 356 171 7 43 363 17 341 1.65e-54 189
Msa0567490 AT3G26490.3 33.427 356 171 7 43 363 17 341 1.65e-54 189
Msa0567490 AT3G26490.4 33.427 356 171 7 43 363 17 341 1.65e-54 189
Msa0567490 AT3G08570.1 33.639 327 180 7 52 362 34 339 2.09e-54 189
Msa0567490 AT1G03010.2 35.015 337 175 8 52 367 9 322 4.58e-54 188
Msa0567490 AT1G03010.1 34.234 333 183 6 52 367 36 349 8.36e-54 188
Msa0567490 AT1G03010.3 35.015 337 175 8 52 367 35 348 9.33e-54 187
Msa0567490 AT3G44820.1 30.548 347 204 6 47 361 18 359 3.72e-53 186
Msa0567490 AT2G47860.6 33.038 339 174 7 52 357 37 355 5.94e-53 182
Msa0567490 AT2G14820.2 31.624 351 185 8 38 361 11 333 2.06e-52 184
Msa0567490 AT2G14820.1 31.624 351 185 8 38 361 11 333 2.06e-52 184
Msa0567490 AT2G47860.3 33.038 339 174 7 52 357 37 355 3.03e-52 184
Msa0567490 AT5G47800.3 31.548 336 195 8 43 362 17 333 5.17e-52 182
Msa0567490 AT5G47800.1 31.548 336 195 8 43 362 17 333 5.17e-52 182
Msa0567490 AT5G47800.4 31.548 336 195 8 43 362 17 333 5.17e-52 182
Msa0567490 AT4G31820.1 31.065 338 180 8 46 356 21 332 6.15e-52 182
Msa0567490 AT5G13600.1 31.549 355 188 9 44 364 18 351 1.24e-51 181
Msa0567490 AT5G47800.2 31.548 336 195 8 43 362 130 446 3.46e-51 181
Msa0567490 AT3G50840.1 31.212 330 195 3 47 359 11 325 1.02e-50 178
Msa0567490 AT2G30520.2 33.447 293 180 5 72 364 2 279 2.89e-49 174
Msa0567490 AT2G30520.3 33.447 293 180 5 72 364 2 279 2.89e-49 174
Msa0567490 AT4G37590.1 30.312 353 183 9 38 356 11 334 4.83e-49 174
Msa0567490 AT3G08660.2 30.938 320 180 6 52 361 34 322 2.22e-47 166
Msa0567490 AT3G08660.1 30.938 320 180 6 52 361 34 322 1.15e-46 167
Msa0567490 AT5G66560.2 28.827 392 181 8 47 359 14 386 2.44e-46 165
Msa0567490 AT5G66560.1 28.827 392 181 8 47 359 14 386 2.81e-45 165
Msa0567490 AT4G31820.2 32.281 285 141 7 99 356 20 279 2.20e-44 160
Msa0567490 AT2G23050.1 28.487 337 192 7 41 356 16 324 1.12e-42 155
Msa0567490 AT3G50840.2 31.915 282 160 3 95 359 23 289 1.45e-42 155
Msa0567490 AT3G50840.3 31.915 282 160 3 95 359 23 289 1.45e-42 155
Msa0567490 AT5G66560.4 31.894 301 150 6 95 359 66 347 2.49e-42 156
Msa0567490 AT5G66560.3 31.894 301 150 6 95 359 76 357 2.78e-42 156
Msa0567490 AT3G08660.3 31.338 284 156 4 88 361 8 262 3.50e-42 154
Msa0567490 AT5G48130.1 34.071 226 120 4 52 254 39 258 1.12e-35 137
Msa0567490 AT5G03250.2 31.200 250 126 5 138 357 1 234 3.17e-31 124
Msa0567490 AT2G47860.5 31.197 234 127 4 138 357 1 214 3.56e-28 115
Msa0567490 AT2G47860.2 31.197 234 127 4 138 357 1 214 3.56e-28 115
Msa0567490 AT3G19850.1 26.367 311 162 5 94 365 51 333 5.36e-26 109
Msa0567490 AT1G52770.1 31.953 169 101 2 197 361 10 168 7.22e-25 105
Msa0567490 AT3G15570.1 31.410 156 83 3 215 359 29 171 7.09e-21 94.0
Msa0567490 AT2G23050.2 25.847 236 130 6 138 356 1 208 1.30e-18 86.7
Msa0567490 AT5G17580.2 24.852 338 195 9 42 356 10 311 2.93e-17 83.6
Msa0567490 AT1G50280.1 25.000 296 166 6 95 356 51 324 7.15e-17 82.4
Msa0567490 AT1G50280.2 25.338 296 165 7 95 356 18 291 9.58e-17 82.0
Msa0567490 AT5G17580.1 25.153 326 185 9 54 356 7 296 1.74e-16 81.3
Msa0567490 AT3G22104.1 24.051 316 166 11 76 357 26 301 1.01e-15 79.0
Msa0567490 AT3G03510.1 25.397 252 148 6 107 356 8 221 7.44e-14 73.2

Find 141 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTGTCAGACTGACTTTC+AGG 0.132980 4_1:-68821259 None:intergenic
CACAGCATCTTCTAACAATT+TGG 0.179939 4_1:-68822540 None:intergenic
CTCCAGCCTGATAGATTCTT+TGG 0.216318 4_1:-68824860 None:intergenic
GAGGAGGAATTATGCTTATT+AGG 0.225056 4_1:-68822894 None:intergenic
ATTCAATATTAGTAGGCTTT+TGG 0.241298 4_1:+68823385 Msa0567490:three_prime_UTR
AAGATAGAAATTAGTGTCTT+TGG 0.247653 4_1:-68823210 None:intergenic
CATTTGTCGTGCTTTCTTCA+TGG 0.250887 4_1:+68822458 Msa0567490:CDS
CAGCCTCTAATCTCAAATTC+TGG 0.250919 4_1:+68822244 Msa0567490:CDS
TTAGATATAATATAAAATTT+AGG 0.251980 4_1:-68825081 None:intergenic
TGCGGATAATTTCAGCAATT+AGG 0.253941 4_1:+68822674 Msa0567490:CDS
AACATTGTAATTGAAAGATT+TGG 0.268935 4_1:-68823437 None:intergenic
TTCCCAGAATTTGAGATTAG+AGG 0.277466 4_1:-68822247 None:intergenic
AAGCTGGTGGTTCGATGATT+TGG 0.289085 4_1:+68822627 Msa0567490:CDS
GACTTTCAGGGTCTTGTGCT+TGG 0.289224 4_1:-68821246 None:intergenic
GCGGATAATTTCAGCAATTA+GGG 0.291903 4_1:+68822675 Msa0567490:CDS
TTAGAAGGGCTTAGAGGATA+TGG 0.292438 4_1:+68822778 Msa0567490:CDS
CTCAAGCTTGAAAACTTTCC+AGG 0.304064 4_1:+68822274 Msa0567490:CDS
TTCTCCTGTCATATTGGAAT+TGG 0.317604 4_1:+68823120 Msa0567490:three_prime_UTR
TATCAGGCTGGAGTTTAAAT+TGG 0.321879 4_1:+68824870 Msa0567490:three_prime_UTR
GCACATTATAAGTGGTTTCC+TGG 0.326824 4_1:-68821562 None:intergenic
TGTCTATAGTTTCAGATGTC+TGG 0.334509 4_1:-68824727 None:intergenic
ACCTGGACAACTGTTCTTAG+AGG 0.338185 4_1:-68823754 None:intergenic
ACTTTCAGGGTCTTGTGCTT+GGG 0.351122 4_1:-68821245 None:intergenic
CCTACTAATATTGAATTTCC+CGG 0.364748 4_1:-68823378 None:intergenic
GAGGAGTTAGAAGGGCTTAG+AGG 0.370662 4_1:+68822772 Msa0567490:CDS
AAATTTAGGCTATATGCTTC+AGG 0.374622 4_1:-68825067 None:intergenic
ACAATTCTAAAATTCTGTTA+TGG 0.376842 4_1:+68822316 Msa0567490:CDS
TGCAGAGTCTTCTACGGTCT+TGG 0.377991 4_1:-68823666 None:intergenic
TTCTACGGTCTTGGTCAGTT+AGG 0.381517 4_1:-68823657 None:intergenic
CCGGGAAATTCAATATTAGT+AGG 0.395757 4_1:+68823378 Msa0567490:three_prime_UTR
TTTACCAATGATCTCTGGTT+TGG 0.398294 4_1:-68822707 None:intergenic
GATGCTGCATCCTCACTTGC+GGG 0.402710 4_1:-68822595 None:intergenic
GACAAACCAAAGAATCTATC+AGG 0.413587 4_1:+68824854 Msa0567490:three_prime_UTR
ACACGGAGAGCCTTCTTCTC+GGG 0.418325 4_1:-68824994 None:intergenic
GAGCTAATTGCTGCAAGCTT+GGG 0.418641 4_1:-68821193 None:intergenic
TCTAACTCCTCATACATTCC+TGG 0.422868 4_1:-68822760 None:intergenic
GACCTTTAGGTAGGTGTCAA+TGG 0.427446 4_1:-68823522 None:intergenic
TTAATTCCTAGATATCAAAA+TGG 0.429080 4_1:+68823054 Msa0567490:CDS
AGCCTCTAATCTCAAATTCT+GGG 0.431924 4_1:+68822245 Msa0567490:CDS
ATGCAACTGCAACAAAAGTC+CGG 0.435207 4_1:+68823359 Msa0567490:three_prime_UTR
TCTGGTTTCATATATCAGTT+AGG 0.435439 4_1:+68821144 Msa0567490:five_prime_UTR
GACACGGAGAGCCTTCTTCT+CGG 0.443418 4_1:-68824995 None:intergenic
TTATCCGAGTTGAAGTCTAT+AGG 0.445995 4_1:-68822343 None:intergenic
TATCCGAGTTGAAGTCTATA+GGG 0.446756 4_1:-68822342 None:intergenic
CTTGAATTGAAAAGTCTGTT+GGG 0.449484 4_1:-68821538 None:intergenic
TTCTGTCAGACTGACTTTCA+GGG 0.449988 4_1:-68821258 None:intergenic
TCTACGGTCTTGGTCAGTTA+GGG 0.449995 4_1:-68823656 None:intergenic
ATTAATTTAGACAGCGTGTC+TGG 0.450562 4_1:+68821126 Msa0567490:five_prime_UTR
TCCTTCCATGTAAAACCAAC+AGG 0.451380 4_1:+68824908 Msa0567490:three_prime_UTR
ATACATTTACCAATGATCTC+TGG 0.452219 4_1:-68822712 None:intergenic
AAGTGGAATCAACTCTCAAC+AGG 0.453660 4_1:+68824697 Msa0567490:three_prime_UTR
TGAGCTAATTGCTGCAAGCT+TGG 0.454672 4_1:-68821194 None:intergenic
TCTCTAGTTTCACCTCCTGT+TGG 0.456759 4_1:-68824923 None:intergenic
TACCTTGTGCACATTATAAG+TGG 0.465167 4_1:-68821570 None:intergenic
GGAATGTATGAGGAGTTAGA+AGG 0.465676 4_1:+68822763 Msa0567490:CDS
CTTGGTCAGTTAGGGAAGGA+TGG 0.466551 4_1:-68823648 None:intergenic
AGCTATTGTGACATGTTTGT+TGG 0.475232 4_1:-68821314 None:intergenic
TGAAATGATCGAGTATGTTC+TGG 0.476508 4_1:-68823482 None:intergenic
ACTTGAATTGAAAAGTCTGT+TGG 0.476835 4_1:-68821539 None:intergenic
ATTTCAGCAATTAGGGCAAA+GGG 0.478066 4_1:+68822682 Msa0567490:CDS
ATATCAAAATGCGGCTACAT+AGG 0.478687 4_1:+68822208 Msa0567490:CDS
ATCTGCAACAAGAACTTACA+TGG 0.493501 4_1:-68822931 None:intergenic
GCCTCTAAGAACAGTTGTCC+AGG 0.494899 4_1:+68823753 Msa0567490:three_prime_UTR
GCTATTGTGACATGTTTGTT+GGG 0.495372 4_1:-68821313 None:intergenic
ACGAGTGTGGTTTGGTGATG+TGG 0.497567 4_1:-68824960 None:intergenic
GATGCTGTGATTCTATAGCT+TGG 0.503818 4_1:+68822554 Msa0567490:CDS
TGTGAGGAAAGCTTCAGTCT+TGG 0.507277 4_1:-68822437 None:intergenic
GGAACCAAACCAGAGATCAT+TGG 0.508422 4_1:+68822703 Msa0567490:CDS
GATCTACGACGAGTGTGGTT+TGG 0.513023 4_1:-68824968 None:intergenic
CTTGTTGCAGATGCTAAAGA+TGG 0.513993 4_1:+68822942 Msa0567490:CDS
GAAAAGTCTGTTGGGATTCG+AGG 0.514401 4_1:-68821530 None:intergenic
AGCAATGCAAGAGAAGGAAC+CGG 0.515645 4_1:+68824670 Msa0567490:three_prime_UTR
CATTCCTGGTAGCCATCTCT+TGG 0.516344 4_1:-68822746 None:intergenic
CTGTGATTCTATAGCTTGGA+AGG 0.517789 4_1:+68822558 Msa0567490:CDS
TCAGACCTTGAAAAGAGAGT+TGG 0.522254 4_1:+68822997 Msa0567490:CDS
GTGTAGAATTGTAGTTAAGA+TGG 0.523176 4_1:-68821081 None:intergenic
CAGGCTGGAGTTTAAATTGG+CGG 0.523456 4_1:+68824873 Msa0567490:three_prime_UTR
TAGAAGGGCTTAGAGGATAT+GGG 0.526409 4_1:+68822779 Msa0567490:CDS
GTAGATCAGCCCCGAGAAGA+AGG 0.527618 4_1:+68824984 Msa0567490:three_prime_UTR
CTTGTGAAAATCTATCTCCA+TGG 0.528028 4_1:+68822506 Msa0567490:CDS
CCTTGAAAAGAGAGTTGGTA+TGG 0.528846 4_1:+68823002 Msa0567490:CDS
AACCAAAGAATCTATCAGGC+TGG 0.531250 4_1:+68824858 Msa0567490:three_prime_UTR
AGATATCAAAATGGAGGTCA+AGG 0.533332 4_1:+68823063 Msa0567490:CDS
TGCAACTGCAACAAAAGTCC+GGG 0.536493 4_1:+68823360 Msa0567490:three_prime_UTR
TTGGTCAGTTAGGGAAGGAT+GGG 0.536654 4_1:-68823647 None:intergenic
AATTTCAGCAATTAGGGCAA+AGG 0.537477 4_1:+68822681 Msa0567490:CDS
AAAAGTCTGTTGGGATTCGA+GGG 0.537749 4_1:-68821529 None:intergenic
AAACATGAAATCACTTCAAG+TGG 0.548709 4_1:+68821222 Msa0567490:exon
AGATGCTGCATCCTCACTTG+CGG 0.555801 4_1:-68822596 None:intergenic
AATGCCAATTCCAATATGAC+AGG 0.558807 4_1:-68823124 None:intergenic
TTGTGAAAATCTATCTCCAT+GGG 0.559597 4_1:+68822507 Msa0567490:CDS
TAAAAGGCTGACCTTTAGGT+AGG 0.562453 4_1:-68823531 None:intergenic
GAGAGCAGCAATGCAAGAGA+AGG 0.563979 4_1:+68824664 Msa0567490:three_prime_UTR
TATGCCAAGAGATGGCTACC+AGG 0.565928 4_1:+68822742 Msa0567490:CDS
GGAATTGGCATTAATGCTGA+AGG 0.568875 4_1:+68823135 Msa0567490:three_prime_UTR
GCAGTAATTCAGCTAAGACT+TGG 0.571771 4_1:-68823460 None:intergenic
ATGACTGAAGAACTAGAAGA+TGG 0.572238 4_1:+68822403 Msa0567490:CDS
GATCTGTTGCAACTTCAGCT+AGG 0.573474 4_1:-68823415 None:intergenic
TAGAATTGTAGTTAAGATGG+TGG 0.574829 4_1:-68821078 None:intergenic
CAGCATCTAATCAAGAAAGC+TGG 0.575400 4_1:+68822611 Msa0567490:CDS
GTTCTGCAGTTCTACCATGT+TGG 0.579053 4_1:-68824786 None:intergenic
GAGTTGATTCCACTTTGTGC+CGG 0.581941 4_1:-68824689 None:intergenic
CACGGAGAGCCTTCTTCTCG+GGG 0.584375 4_1:-68824993 None:intergenic
ATGGCTACCAGGAATGTATG+AGG 0.585069 4_1:+68822753 Msa0567490:CDS
TCAAATGTTTCACATCCACC+TGG 0.585855 4_1:-68822292 None:intergenic
AGCCATTGACACCTACCTAA+AGG 0.587167 4_1:+68823520 Msa0567490:three_prime_UTR
TCATGCAATATGCCAAGAGA+TGG 0.594644 4_1:+68822734 Msa0567490:CDS
CATGGGACAGCATCTTGTTG+AGG 0.601532 4_1:-68822913 None:intergenic
AGAGAGCACTAGACATAGCA+AGG 0.603854 4_1:+68822849 Msa0567490:CDS
CGAGTGTGGTTTGGTGATGT+GGG 0.605082 4_1:-68824959 None:intergenic
GGGCTTAGAGGATATGGGCA+TGG 0.605115 4_1:+68822784 Msa0567490:CDS
GAATGTATGAGGAGTTAGAA+GGG 0.605950 4_1:+68822764 Msa0567490:CDS
CGGTCTTGGTCAGTTAGGGA+AGG 0.612259 4_1:-68823652 None:intergenic
CGAGAATGTAAAGTCCAACA+TGG 0.615865 4_1:+68824772 Msa0567490:three_prime_UTR
CATAAAATGATCAATGCGGA+GGG 0.617020 4_1:-68822653 None:intergenic
GGGACAGCATCTTGTTGAGG+AGG 0.618679 4_1:-68822910 None:intergenic
GAGAAGGAACCGGCACAAAG+TGG 0.620698 4_1:+68824680 Msa0567490:three_prime_UTR
ATTCCTAGATATCAAAATGG+AGG 0.624406 4_1:+68823057 Msa0567490:CDS
AAAATGATCAATGCGGAGGG+TGG 0.627548 4_1:-68822650 None:intergenic
CAAAATGCGGCTACATAGGA+CGG 0.632260 4_1:+68822212 Msa0567490:CDS
AGAATTGTAGTTAAGATGGT+GGG 0.638014 4_1:-68821077 None:intergenic
ATGCGGCTACATAGGACGGT+CGG 0.639956 4_1:+68822216 Msa0567490:CDS
GCATAAAATGATCAATGCGG+AGG 0.642568 4_1:-68822654 None:intergenic
ACAGGAGGTGAAACTAGAGA+TGG 0.647018 4_1:+68824926 Msa0567490:three_prime_UTR
AAGAAAGCACGACAAATGTG+AGG 0.659530 4_1:-68822453 None:intergenic
AAGCTTGAAAACTTTCCAGG+TGG 0.660259 4_1:+68822277 Msa0567490:CDS
CATCTAATCAAGAAAGCTGG+TGG 0.660897 4_1:+68822614 Msa0567490:CDS
AACCACTTATAATGTGCACA+AGG 0.667560 4_1:+68821568 Msa0567490:CDS
CCTCCCTATAGACTTCAACT+CGG 0.675286 4_1:+68822339 Msa0567490:CDS
GGGCTGATCTACGACGAGTG+TGG 0.675575 4_1:-68824973 None:intergenic
TAATGCTGAGTACTGTTCTG+TGG 0.676472 4_1:-68821107 None:intergenic
CCGAGTTGAAGTCTATAGGG+AGG 0.687521 4_1:-68822339 None:intergenic
CACTATGCAAGACATAGATG+TGG 0.688304 4_1:+68823292 Msa0567490:three_prime_UTR
TCTGCAACAAGAACTTACAT+GGG 0.692050 4_1:-68822930 None:intergenic
AAAGTCTGTTGGGATTCGAG+GGG 0.692715 4_1:-68821528 None:intergenic
AAGACATAGATGTGGTACAA+CGG 0.695512 4_1:+68823300 Msa0567490:three_prime_UTR
TTCCATGTAAAACCAACAGG+AGG 0.698959 4_1:+68824911 Msa0567490:three_prime_UTR
TACCCCTTGATATCAAAATG+CGG 0.703247 4_1:+68822199 Msa0567490:CDS
TCCGCATAAAATGATCAATG+CGG 0.719094 4_1:-68822657 None:intergenic
TATGGTGTTAGAAGATGCTG+AGG 0.726855 4_1:+68823020 Msa0567490:CDS
CATACTCGATCATTTCACGA+TGG 0.744904 4_1:+68823488 Msa0567490:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTATCTTATTAAAAA+TGG - chr4_1:68824441-68824460 None:intergenic 10.0%
!! TTTAACTGCATAAAATATAA+AGG - chr4_1:68821385-68821404 None:intergenic 15.0%
!!! TATTTTCTCTGTTTATTTTT+TGG + chr4_1:68821482-68821501 Msa0567490:intron 15.0%
!!! TTTATATTTTTCCATTTGTA+AGG - chr4_1:68824142-68824161 None:intergenic 15.0%
!!! ATATAAAGGATTCTATTTCT+GGG - chr4_1:68821371-68821390 None:intergenic 20.0%
!! AATATAAAGGATTCTATTTC+TGG - chr4_1:68821372-68821391 None:intergenic 20.0%
!! ACTCATACAAAAATAACTAT+TGG - chr4_1:68821458-68821477 None:intergenic 20.0%
!!! AATGATTTTCTGCATTTATA+AGG - chr4_1:68821614-68821633 None:intergenic 20.0%
!!! TGGTTTTCAAAAAAATTCTA+AGG - chr4_1:68822063-68822082 None:intergenic 20.0%
!! CAACATATAAAAGATTAAGT+TGG - chr4_1:68822160-68822179 None:intergenic 20.0%
!!! CAACTTAATCTTTTATATGT+TGG + chr4_1:68822158-68822177 Msa0567490:intron 20.0%
!!! ACAATTCTAAAATTCTGTTA+TGG + chr4_1:68822316-68822335 Msa0567490:CDS 20.0%
!! TTAATTCCTAGATATCAAAA+TGG + chr4_1:68823054-68823073 Msa0567490:CDS 20.0%
!! TGCGAAAAATAAAAAATAAC+CGG + chr4_1:68823171-68823190 Msa0567490:three_prime_UTR 20.0%
!! AACATTGTAATTGAAAGATT+TGG - chr4_1:68823440-68823459 None:intergenic 20.0%
!! ACAAGAAAATAGGAATTAAA+AGG - chr4_1:68823550-68823569 None:intergenic 20.0%
!!! GATTTCTTTTTCAAAGTTAA+AGG - chr4_1:68824015-68824034 None:intergenic 20.0%
!!! TTTTATTTCTAAATCACAGT+AGG - chr4_1:68824300-68824319 None:intergenic 20.0%
!! TATCTTATTAAAAATGGAGT+TGG - chr4_1:68824435-68824454 None:intergenic 20.0%
!!! GATGAAATAATTTTAAGACA+CGG - chr4_1:68825014-68825033 None:intergenic 20.0%
! GCATAAAAAATGAACATTCA+AGG + chr4_1:68821341-68821360 Msa0567490:CDS 25.0%
!!! TCTCTGTTTATTTTTTGGTT+TGG + chr4_1:68821487-68821506 Msa0567490:intron 25.0%
!!! CTCTGTTTATTTTTTGGTTT+GGG + chr4_1:68821488-68821507 Msa0567490:intron 25.0%
!!! CCATGTTTTTATTCTAATTC+TGG - chr4_1:68822083-68822102 None:intergenic 25.0%
! CCAGAATTAGAATAAAAACA+TGG + chr4_1:68822080-68822099 Msa0567490:intron 25.0%
!!! TTTTTTATTTTTCGCAGAGT+TGG - chr4_1:68823167-68823186 None:intergenic 25.0%
! GAAAAATAAAAAATAACCGG+AGG + chr4_1:68823174-68823193 Msa0567490:three_prime_UTR 25.0%
! AAGATAGAAATTAGTGTCTT+TGG - chr4_1:68823213-68823232 None:intergenic 25.0%
!! ATTCAATATTAGTAGGCTTT+TGG + chr4_1:68823385-68823404 Msa0567490:three_prime_UTR 25.0%
! TGAATTGAACACAAGAAAAT+AGG - chr4_1:68823560-68823579 None:intergenic 25.0%
!! TATTTTCTTGTGTTCAATTC+AGG + chr4_1:68823559-68823578 Msa0567490:intron 25.0%
! GGAATTCTTAAAACTCTATT+TGG - chr4_1:68823836-68823855 None:intergenic 25.0%
!!! TGTCATCATTTTTTTCTTCA+TGG - chr4_1:68823857-68823876 None:intergenic 25.0%
! AAAGAAGAAACTTCTTTAGA+AGG + chr4_1:68824078-68824097 Msa0567490:intron 25.0%
AGAATTGTAGTTAAGATGGT+GGG - chr4_1:68821080-68821099 None:intergenic 30.0%
TAGAATTGTAGTTAAGATGG+TGG - chr4_1:68821081-68821100 None:intergenic 30.0%
GTGTAGAATTGTAGTTAAGA+TGG - chr4_1:68821084-68821103 None:intergenic 30.0%
! TCTGGTTTCATATATCAGTT+AGG + chr4_1:68821144-68821163 Msa0567490:five_prime_UTR 30.0%
AAACATGAAATCACTTCAAG+TGG + chr4_1:68821222-68821241 Msa0567490:exon 30.0%
CTTGAATTGAAAAGTCTGTT+GGG - chr4_1:68821541-68821560 None:intergenic 30.0%
ACTTGAATTGAAAAGTCTGT+TGG - chr4_1:68821542-68821561 None:intergenic 30.0%
! TTTTACTGAGCTAAACAAGA+AGG - chr4_1:68821639-68821658 None:intergenic 30.0%
!! TATTTTGCATGCTTTTAAGG+AGG - chr4_1:68821953-68821972 None:intergenic 30.0%
!!! TGCTATTTTGCATGCTTTTA+AGG - chr4_1:68821956-68821975 None:intergenic 30.0%
TTGTGAAAATCTATCTCCAT+GGG + chr4_1:68822507-68822526 Msa0567490:CDS 30.0%
ATACATTTACCAATGATCTC+TGG - chr4_1:68822715-68822734 None:intergenic 30.0%
ATTCCTAGATATCAAAATGG+AGG + chr4_1:68823057-68823076 Msa0567490:CDS 30.0%
!! TCATTTTTCTCCTGTCATAT+TGG + chr4_1:68823114-68823133 Msa0567490:three_prime_UTR 30.0%
CCTACTAATATTGAATTTCC+CGG - chr4_1:68823381-68823400 None:intergenic 30.0%
!! TTATTTTGCAGAGTCTTCTA+CGG - chr4_1:68823675-68823694 None:intergenic 30.0%
!! TGTGTTGTTGTGTTTTTCAA+AGG - chr4_1:68823924-68823943 None:intergenic 30.0%
ATTGAAGATCTGAACCAATT+TGG + chr4_1:68823944-68823963 Msa0567490:intron 30.0%
TTGAAGATCTGAACCAATTT+GGG + chr4_1:68823945-68823964 Msa0567490:intron 30.0%
! AAACTTCTTTAGAAGGAAAG+AGG + chr4_1:68824085-68824104 Msa0567490:intron 30.0%
! AACTTCTTTAGAAGGAAAGA+GGG + chr4_1:68824086-68824105 Msa0567490:intron 30.0%
GTACAAATGATCCTTACAAA+TGG + chr4_1:68824128-68824147 Msa0567490:intron 30.0%
! ACTCTATGACACATGATTTT+GGG - chr4_1:68824206-68824225 None:intergenic 30.0%
TGTATAGAGGAAATACTTTG+CGG - chr4_1:68824255-68824274 None:intergenic 30.0%
! ACACAAACTTTTGTGTATAG+AGG - chr4_1:68824268-68824287 None:intergenic 30.0%
AATTCCACAAAACAACATTG+TGG + chr4_1:68824459-68824478 Msa0567490:intron 30.0%
AAATTTAGGCTATATGCTTC+AGG - chr4_1:68825070-68825089 None:intergenic 30.0%
ATTAATTTAGACAGCGTGTC+TGG + chr4_1:68821126-68821145 Msa0567490:five_prime_UTR 35.0%
GCTATTGTGACATGTTTGTT+GGG - chr4_1:68821316-68821335 None:intergenic 35.0%
AGCTATTGTGACATGTTTGT+TGG - chr4_1:68821317-68821336 None:intergenic 35.0%
! AGACTTTTCAATTCAAGTCC+AGG + chr4_1:68821544-68821563 Msa0567490:CDS 35.0%
AACCACTTATAATGTGCACA+AGG + chr4_1:68821568-68821587 Msa0567490:CDS 35.0%
TACCTTGTGCACATTATAAG+TGG - chr4_1:68821573-68821592 None:intergenic 35.0%
! AAGAGCACAATCCTGTTAAT+AGG + chr4_1:68821766-68821785 Msa0567490:intron 35.0%
AGAGCACAATCCTGTTAATA+GGG + chr4_1:68821767-68821786 Msa0567490:intron 35.0%
GATTTATGCTCCCTATTAAC+AGG - chr4_1:68821780-68821799 None:intergenic 35.0%
! CTCTTTTGGATAGAGAAAGA+GGG + chr4_1:68821873-68821892 Msa0567490:intron 35.0%
! TCTTTTGGATAGAGAAAGAG+GGG + chr4_1:68821874-68821893 Msa0567490:intron 35.0%
TACCCCTTGATATCAAAATG+CGG + chr4_1:68822199-68822218 Msa0567490:CDS 35.0%
!! TAGCCGCATTTTGATATCAA+GGG - chr4_1:68822205-68822224 None:intergenic 35.0%
ATATCAAAATGCGGCTACAT+AGG + chr4_1:68822208-68822227 Msa0567490:CDS 35.0%
AGCCTCTAATCTCAAATTCT+GGG + chr4_1:68822245-68822264 Msa0567490:CDS 35.0%
! TTCCCAGAATTTGAGATTAG+AGG - chr4_1:68822250-68822269 None:intergenic 35.0%
! TATCCGAGTTGAAGTCTATA+GGG - chr4_1:68822345-68822364 None:intergenic 35.0%
! TTATCCGAGTTGAAGTCTAT+AGG - chr4_1:68822346-68822365 None:intergenic 35.0%
ATGACTGAAGAACTAGAAGA+TGG + chr4_1:68822403-68822422 Msa0567490:CDS 35.0%
CTTGTGAAAATCTATCTCCA+TGG + chr4_1:68822506-68822525 Msa0567490:CDS 35.0%
! ATTTGGATATTTTCAGCCCA+TGG - chr4_1:68822526-68822545 None:intergenic 35.0%
CACAGCATCTTCTAACAATT+TGG - chr4_1:68822543-68822562 None:intergenic 35.0%
CATAAAATGATCAATGCGGA+GGG - chr4_1:68822656-68822675 None:intergenic 35.0%
!! TCCGCATTGATCATTTTATG+CGG + chr4_1:68822656-68822675 Msa0567490:CDS 35.0%
TCCGCATAAAATGATCAATG+CGG - chr4_1:68822660-68822679 None:intergenic 35.0%
TGCGGATAATTTCAGCAATT+AGG + chr4_1:68822674-68822693 Msa0567490:CDS 35.0%
GCGGATAATTTCAGCAATTA+GGG + chr4_1:68822675-68822694 Msa0567490:CDS 35.0%
AATTTCAGCAATTAGGGCAA+AGG + chr4_1:68822681-68822700 Msa0567490:CDS 35.0%
ATTTCAGCAATTAGGGCAAA+GGG + chr4_1:68822682-68822701 Msa0567490:CDS 35.0%
TTTACCAATGATCTCTGGTT+TGG - chr4_1:68822710-68822729 None:intergenic 35.0%
GAATGTATGAGGAGTTAGAA+GGG + chr4_1:68822764-68822783 Msa0567490:CDS 35.0%
GAGGAGGAATTATGCTTATT+AGG - chr4_1:68822897-68822916 None:intergenic 35.0%
TCTGCAACAAGAACTTACAT+GGG - chr4_1:68822933-68822952 None:intergenic 35.0%
ATCTGCAACAAGAACTTACA+TGG - chr4_1:68822934-68822953 None:intergenic 35.0%
!! TGACCTCCATTTTGATATCT+AGG - chr4_1:68823063-68823082 None:intergenic 35.0%
AGATATCAAAATGGAGGTCA+AGG + chr4_1:68823063-68823082 Msa0567490:CDS 35.0%
AAATGGAGGTCAAGGAAAAA+TGG + chr4_1:68823071-68823090 Msa0567490:CDS 35.0%
TTCTCCTGTCATATTGGAAT+TGG + chr4_1:68823120-68823139 Msa0567490:three_prime_UTR 35.0%
AATGCCAATTCCAATATGAC+AGG - chr4_1:68823127-68823146 None:intergenic 35.0%
AAGACATAGATGTGGTACAA+CGG + chr4_1:68823300-68823319 Msa0567490:three_prime_UTR 35.0%
CCGGGAAATTCAATATTAGT+AGG + chr4_1:68823378-68823397 Msa0567490:three_prime_UTR 35.0%
TGAAATGATCGAGTATGTTC+TGG - chr4_1:68823485-68823504 None:intergenic 35.0%
GAATTAAAAGGCTGACCTTT+AGG - chr4_1:68823538-68823557 None:intergenic 35.0%
!! GATCTTTGGAACTTAGTCTT+CGG + chr4_1:68823788-68823807 Msa0567490:intron 35.0%
TGAAGATCTGAACCAATTTG+GGG + chr4_1:68823946-68823965 Msa0567490:intron 35.0%
AACCAAACAGCAAGACTAAT+TGG + chr4_1:68824160-68824179 Msa0567490:intron 35.0%
!! TTCCAATTAGTCTTGCTGTT+TGG - chr4_1:68824165-68824184 None:intergenic 35.0%
! CTCTATGACACATGATTTTG+GGG - chr4_1:68824205-68824224 None:intergenic 35.0%
! CACTCTATGACACATGATTT+TGG - chr4_1:68824207-68824226 None:intergenic 35.0%
ATCATGTGTCATAGAGTGAA+AGG + chr4_1:68824209-68824228 Msa0567490:intron 35.0%
TCATGTGTCATAGAGTGAAA+GGG + chr4_1:68824210-68824229 Msa0567490:intron 35.0%
!!! AGATCCACAATGTTGTTTTG+TGG - chr4_1:68824466-68824485 None:intergenic 35.0%
ACAAAACAACATTGTGGATC+TGG + chr4_1:68824465-68824484 Msa0567490:intron 35.0%
!! TGGACATATTAGCTCTGAAA+AGG - chr4_1:68824566-68824585 None:intergenic 35.0%
CAGAGCTAATATGTCCATAA+TGG + chr4_1:68824569-68824588 Msa0567490:intron 35.0%
TCTAAGCTGAATCACCATTA+TGG - chr4_1:68824586-68824605 None:intergenic 35.0%
TGTCTATAGTTTCAGATGTC+TGG - chr4_1:68824730-68824749 None:intergenic 35.0%
GACAAACCAAAGAATCTATC+AGG + chr4_1:68824854-68824873 Msa0567490:three_prime_UTR 35.0%
TATCAGGCTGGAGTTTAAAT+TGG + chr4_1:68824870-68824889 Msa0567490:three_prime_UTR 35.0%
! TAATGCTGAGTACTGTTCTG+TGG - chr4_1:68821110-68821129 None:intergenic 40.0%
! TTCTGTCAGACTGACTTTCA+GGG - chr4_1:68821261-68821280 None:intergenic 40.0%
!!! TTTTTGGTTTGGGCTTATGC+AGG + chr4_1:68821498-68821517 Msa0567490:intron 40.0%
AAAAGTCTGTTGGGATTCGA+GGG - chr4_1:68821532-68821551 None:intergenic 40.0%
GCACATTATAAGTGGTTTCC+TGG - chr4_1:68821565-68821584 None:intergenic 40.0%
TTCCAAACAGTATCATCAGC+AGG + chr4_1:68821662-68821681 Msa0567490:intron 40.0%
TCCAAACAGTATCATCAGCA+GGG + chr4_1:68821663-68821682 Msa0567490:intron 40.0%
TATCATCAGCAGGGTATATG+AGG + chr4_1:68821672-68821691 Msa0567490:intron 40.0%
ATCATCAGCAGGGTATATGA+GGG + chr4_1:68821673-68821692 Msa0567490:intron 40.0%
CACAAACACACTAGGAAGAA+AGG - chr4_1:68821810-68821829 None:intergenic 40.0%
CTTTCTCTATCCAAAAGAGG+TGG - chr4_1:68821872-68821891 None:intergenic 40.0%
CCTCTTTCTCTATCCAAAAG+AGG - chr4_1:68821875-68821894 None:intergenic 40.0%
! CCTCTTTTGGATAGAGAAAG+AGG + chr4_1:68821872-68821891 Msa0567490:intron 40.0%
! CTTTTGGATAGAGAAAGAGG+GGG + chr4_1:68821875-68821894 Msa0567490:intron 40.0%
TAGACAGAGAACATCAGACA+TGG + chr4_1:68821918-68821937 Msa0567490:intron 40.0%
AGAGAACATCAGACATGGAT+AGG + chr4_1:68821923-68821942 Msa0567490:intron 40.0%
GAGAACATCAGACATGGATA+GGG + chr4_1:68821924-68821943 Msa0567490:intron 40.0%
!! AGCCGCATTTTGATATCAAG+GGG - chr4_1:68822204-68822223 None:intergenic 40.0%
!! GTAGCCGCATTTTGATATCA+AGG - chr4_1:68822206-68822225 None:intergenic 40.0%
CAGCCTCTAATCTCAAATTC+TGG + chr4_1:68822244-68822263 Msa0567490:CDS 40.0%
CTCAAGCTTGAAAACTTTCC+AGG + chr4_1:68822274-68822293 Msa0567490:CDS 40.0%
AAGCTTGAAAACTTTCCAGG+TGG + chr4_1:68822277-68822296 Msa0567490:CDS 40.0%
TCAAATGTTTCACATCCACC+TGG - chr4_1:68822295-68822314 None:intergenic 40.0%
! AAGAAAGCACGACAAATGTG+AGG - chr4_1:68822456-68822475 None:intergenic 40.0%
CATTTGTCGTGCTTTCTTCA+TGG + chr4_1:68822458-68822477 Msa0567490:CDS 40.0%
GATGCTGTGATTCTATAGCT+TGG + chr4_1:68822554-68822573 Msa0567490:CDS 40.0%
CTGTGATTCTATAGCTTGGA+AGG + chr4_1:68822558-68822577 Msa0567490:CDS 40.0%
CAGCATCTAATCAAGAAAGC+TGG + chr4_1:68822611-68822630 Msa0567490:CDS 40.0%
CATCTAATCAAGAAAGCTGG+TGG + chr4_1:68822614-68822633 Msa0567490:CDS 40.0%
GCATAAAATGATCAATGCGG+AGG - chr4_1:68822657-68822676 None:intergenic 40.0%
TCATGCAATATGCCAAGAGA+TGG + chr4_1:68822734-68822753 Msa0567490:CDS 40.0%
TCTAACTCCTCATACATTCC+TGG - chr4_1:68822763-68822782 None:intergenic 40.0%
GGAATGTATGAGGAGTTAGA+AGG + chr4_1:68822763-68822782 Msa0567490:CDS 40.0%
TTAGAAGGGCTTAGAGGATA+TGG + chr4_1:68822778-68822797 Msa0567490:CDS 40.0%
TAGAAGGGCTTAGAGGATAT+GGG + chr4_1:68822779-68822798 Msa0567490:CDS 40.0%
CTTGTTGCAGATGCTAAAGA+TGG + chr4_1:68822942-68822961 Msa0567490:CDS 40.0%
TCAGACCTTGAAAAGAGAGT+TGG + chr4_1:68822997-68823016 Msa0567490:CDS 40.0%
! CCATACCAACTCTCTTTTCA+AGG - chr4_1:68823005-68823024 None:intergenic 40.0%
!! CCTTGAAAAGAGAGTTGGTA+TGG + chr4_1:68823002-68823021 Msa0567490:CDS 40.0%
! TATGGTGTTAGAAGATGCTG+AGG + chr4_1:68823020-68823039 Msa0567490:CDS 40.0%
GGAATTGGCATTAATGCTGA+AGG + chr4_1:68823135-68823154 Msa0567490:three_prime_UTR 40.0%
!! TGGTCATTTTCATAAGCCTC+CGG - chr4_1:68823193-68823212 None:intergenic 40.0%
! TCTTGCATAGTGCAGTTTTC+GGG - chr4_1:68823284-68823303 None:intergenic 40.0%
! GTCTTGCATAGTGCAGTTTT+CGG - chr4_1:68823285-68823304 None:intergenic 40.0%
CACTATGCAAGACATAGATG+TGG + chr4_1:68823292-68823311 Msa0567490:three_prime_UTR 40.0%
ATGCAACTGCAACAAAAGTC+CGG + chr4_1:68823359-68823378 Msa0567490:three_prime_UTR 40.0%
GCAGTAATTCAGCTAAGACT+TGG - chr4_1:68823463-68823482 None:intergenic 40.0%
CATACTCGATCATTTCACGA+TGG + chr4_1:68823488-68823507 Msa0567490:three_prime_UTR 40.0%
TAAAAGGCTGACCTTTAGGT+AGG - chr4_1:68823534-68823553 None:intergenic 40.0%
GACTAAGTTCCAAAGATCCA+GGG - chr4_1:68823786-68823805 None:intergenic 40.0%
AGACTAAGTTCCAAAGATCC+AGG - chr4_1:68823787-68823806 None:intergenic 40.0%
GGTATTTAAGCAGATGCTAG+TGG - chr4_1:68823903-68823922 None:intergenic 40.0%
GAAAACATCTGACCCCAAAT+TGG - chr4_1:68823961-68823980 None:intergenic 40.0%
! AGTTTTCCATCTGCATGGAA+GGG - chr4_1:68824037-68824056 None:intergenic 40.0%
! TAGTTTTCCATCTGCATGGA+AGG - chr4_1:68824038-68824057 None:intergenic 40.0%
!!! TCACTAGTTTTCCATCTGCA+TGG - chr4_1:68824042-68824061 None:intergenic 40.0%
!! ATGACACATGATTTTGGGGA+GGG - chr4_1:68824201-68824220 None:intergenic 40.0%
!! TATGACACATGATTTTGGGG+AGG - chr4_1:68824202-68824221 None:intergenic 40.0%
! GTGGATCTGGTTCACATTAT+AGG + chr4_1:68824478-68824497 Msa0567490:intron 40.0%
! TCTGGTTCACATTATAGGCA+TGG + chr4_1:68824483-68824502 Msa0567490:intron 40.0%
! TGCGTTGTATACTTTGGTTC+AGG - chr4_1:68824617-68824636 None:intergenic 40.0%
TGAACCAAAGTATACAACGC+AGG + chr4_1:68824616-68824635 Msa0567490:intron 40.0%
AGAACCTGCGTTGTATACTT+TGG - chr4_1:68824623-68824642 None:intergenic 40.0%
AAGTGGAATCAACTCTCAAC+AGG + chr4_1:68824697-68824716 Msa0567490:three_prime_UTR 40.0%
CGAGAATGTAAAGTCCAACA+TGG + chr4_1:68824772-68824791 Msa0567490:three_prime_UTR 40.0%
AACCAAAGAATCTATCAGGC+TGG + chr4_1:68824858-68824877 Msa0567490:three_prime_UTR 40.0%
TCCTTCCATGTAAAACCAAC+AGG + chr4_1:68824908-68824927 Msa0567490:three_prime_UTR 40.0%
!!! TCCTGTTGGTTTTACATGGA+AGG - chr4_1:68824912-68824931 None:intergenic 40.0%
TTCCATGTAAAACCAACAGG+AGG + chr4_1:68824911-68824930 Msa0567490:three_prime_UTR 40.0%
GAGCTAATTGCTGCAAGCTT+GGG - chr4_1:68821196-68821215 None:intergenic 45.0%
TGAGCTAATTGCTGCAAGCT+TGG - chr4_1:68821197-68821216 None:intergenic 45.0%
! ACTTTCAGGGTCTTGTGCTT+GGG - chr4_1:68821248-68821267 None:intergenic 45.0%
! GTTCTGTCAGACTGACTTTC+AGG - chr4_1:68821262-68821281 None:intergenic 45.0%
AAAGTCTGTTGGGATTCGAG+GGG - chr4_1:68821531-68821550 None:intergenic 45.0%
GAAAAGTCTGTTGGGATTCG+AGG - chr4_1:68821533-68821552 None:intergenic 45.0%
ACCCTGCTGATGATACTGTT+TGG - chr4_1:68821667-68821686 None:intergenic 45.0%
CACACACACACAAACACACT+AGG - chr4_1:68821818-68821837 None:intergenic 45.0%
TCTATCCAAAAGAGGTGGTG+GGG - chr4_1:68821867-68821886 None:intergenic 45.0%
CTCTATCCAAAAGAGGTGGT+GGG - chr4_1:68821868-68821887 None:intergenic 45.0%
TCTCTATCCAAAAGAGGTGG+TGG - chr4_1:68821869-68821888 None:intergenic 45.0%
GGGAAAATTTCCCTCAAGAG+AGG + chr4_1:68821895-68821914 Msa0567490:intron 45.0%
TCTCTGTCTACCTCTCTTGA+GGG - chr4_1:68821908-68821927 None:intergenic 45.0%
TTCTCTGTCTACCTCTCTTG+AGG - chr4_1:68821909-68821928 None:intergenic 45.0%
CAAAATGCGGCTACATAGGA+CGG + chr4_1:68822212-68822231 Msa0567490:CDS 45.0%
CCTCCCTATAGACTTCAACT+CGG + chr4_1:68822339-68822358 Msa0567490:CDS 45.0%
TGTGAGGAAAGCTTCAGTCT+TGG - chr4_1:68822440-68822459 None:intergenic 45.0%
TAAAAATGAGCCCGCAAGTG+AGG + chr4_1:68822585-68822604 Msa0567490:CDS 45.0%
AAGCTGGTGGTTCGATGATT+TGG + chr4_1:68822627-68822646 Msa0567490:CDS 45.0%
AAAATGATCAATGCGGAGGG+TGG - chr4_1:68822653-68822672 None:intergenic 45.0%
GGAACCAAACCAGAGATCAT+TGG + chr4_1:68822703-68822722 Msa0567490:CDS 45.0%
ATGGCTACCAGGAATGTATG+AGG + chr4_1:68822753-68822772 Msa0567490:CDS 45.0%
!! AGAGAGCACTAGACATAGCA+AGG + chr4_1:68822849-68822868 Msa0567490:CDS 45.0%
!!! GTGCAGTTTTCGGGTGAATT+AGG - chr4_1:68823275-68823294 None:intergenic 45.0%
TGCAACTGCAACAAAAGTCC+GGG + chr4_1:68823360-68823379 Msa0567490:three_prime_UTR 45.0%
GATCTGTTGCAACTTCAGCT+AGG - chr4_1:68823418-68823437 None:intergenic 45.0%
AGCCATTGACACCTACCTAA+AGG + chr4_1:68823520-68823539 Msa0567490:three_prime_UTR 45.0%
GACCTTTAGGTAGGTGTCAA+TGG - chr4_1:68823525-68823544 None:intergenic 45.0%
TTGGTCAGTTAGGGAAGGAT+GGG - chr4_1:68823650-68823669 None:intergenic 45.0%
TCTACGGTCTTGGTCAGTTA+GGG - chr4_1:68823659-68823678 None:intergenic 45.0%
TTCTACGGTCTTGGTCAGTT+AGG - chr4_1:68823660-68823679 None:intergenic 45.0%
ACCTGGACAACTGTTCTTAG+AGG - chr4_1:68823757-68823776 None:intergenic 45.0%
AAGATCCAGGGAACTTTACC+TGG - chr4_1:68823774-68823793 None:intergenic 45.0%
GGTAAAGTTCCCTGGATCTT+TGG + chr4_1:68823774-68823793 Msa0567490:intron 45.0%
AGAAATCCCTTCCATGCAGA+TGG + chr4_1:68824028-68824047 Msa0567490:intron 45.0%
! GCATGGCATCTAAGATAGTG+TGG + chr4_1:68824500-68824519 Msa0567490:intron 45.0%
AGCAATGCAAGAGAAGGAAC+CGG + chr4_1:68824670-68824689 Msa0567490:three_prime_UTR 45.0%
! GAGTTGATTCCACTTTGTGC+CGG - chr4_1:68824692-68824711 None:intergenic 45.0%
GTTCTGCAGTTCTACCATGT+TGG - chr4_1:68824789-68824808 None:intergenic 45.0%
CTCCAGCCTGATAGATTCTT+TGG - chr4_1:68824863-68824882 None:intergenic 45.0%
CAGGCTGGAGTTTAAATTGG+CGG + chr4_1:68824873-68824892 Msa0567490:three_prime_UTR 45.0%
!!! CACCTCCTGTTGGTTTTACA+TGG - chr4_1:68824916-68824935 None:intergenic 45.0%
! TCTCTAGTTTCACCTCCTGT+TGG - chr4_1:68824926-68824945 None:intergenic 45.0%
ACAGGAGGTGAAACTAGAGA+TGG + chr4_1:68824926-68824945 Msa0567490:three_prime_UTR 45.0%
!! TTAGATATAATATAAAATTT+AGG - chr4_1:68825084-68825103 None:intergenic 5.0%
! GACTTTCAGGGTCTTGTGCT+TGG - chr4_1:68821249-68821268 None:intergenic 50.0%
! CCGAGTTGAAGTCTATAGGG+AGG - chr4_1:68822342-68822361 None:intergenic 50.0%
AGATGCTGCATCCTCACTTG+CGG - chr4_1:68822599-68822618 None:intergenic 50.0%
TATGCCAAGAGATGGCTACC+AGG + chr4_1:68822742-68822761 Msa0567490:CDS 50.0%
CATTCCTGGTAGCCATCTCT+TGG - chr4_1:68822749-68822768 None:intergenic 50.0%
GAGGAGTTAGAAGGGCTTAG+AGG + chr4_1:68822772-68822791 Msa0567490:CDS 50.0%
CATGGGACAGCATCTTGTTG+AGG - chr4_1:68822916-68822935 None:intergenic 50.0%
CTTGGTCAGTTAGGGAAGGA+TGG - chr4_1:68823651-68823670 None:intergenic 50.0%
TGCAGAGTCTTCTACGGTCT+TGG - chr4_1:68823669-68823688 None:intergenic 50.0%
! GCCTCTAAGAACAGTTGTCC+AGG + chr4_1:68823753-68823772 Msa0567490:three_prime_UTR 50.0%
GTTGTCCAGGTAAAGTTCCC+TGG + chr4_1:68823766-68823785 Msa0567490:intron 50.0%
GAGAGCAGCAATGCAAGAGA+AGG + chr4_1:68824664-68824683 Msa0567490:three_prime_UTR 50.0%
!! CGAGTGTGGTTTGGTGATGT+GGG - chr4_1:68824962-68824981 None:intergenic 50.0%
!! ACGAGTGTGGTTTGGTGATG+TGG - chr4_1:68824963-68824982 None:intergenic 50.0%
GATCTACGACGAGTGTGGTT+TGG - chr4_1:68824971-68824990 None:intergenic 50.0%
!! TCCAAAAGAGGTGGTGGGGT+GGG - chr4_1:68821863-68821882 None:intergenic 55.0%
!! ATCCAAAAGAGGTGGTGGGG+TGG - chr4_1:68821864-68821883 None:intergenic 55.0%
ATGCGGCTACATAGGACGGT+CGG + chr4_1:68822216-68822235 Msa0567490:CDS 55.0%
GATGCTGCATCCTCACTTGC+GGG - chr4_1:68822598-68822617 None:intergenic 55.0%
GGGCTTAGAGGATATGGGCA+TGG + chr4_1:68822784-68822803 Msa0567490:CDS 55.0%
GGGACAGCATCTTGTTGAGG+AGG - chr4_1:68822913-68822932 None:intergenic 55.0%
CGGTCTTGGTCAGTTAGGGA+AGG - chr4_1:68823655-68823674 None:intergenic 55.0%
GAGAAGGAACCGGCACAAAG+TGG + chr4_1:68824680-68824699 Msa0567490:three_prime_UTR 55.0%
GTAGATCAGCCCCGAGAAGA+AGG + chr4_1:68824984-68825003 Msa0567490:three_prime_UTR 55.0%
ACACGGAGAGCCTTCTTCTC+GGG - chr4_1:68824997-68825016 None:intergenic 55.0%
GACACGGAGAGCCTTCTTCT+CGG - chr4_1:68824998-68825017 None:intergenic 55.0%
! TCCCACCCCACCACCTCTTT+TGG + chr4_1:68821859-68821878 Msa0567490:intron 60.0%
GGGCTGATCTACGACGAGTG+TGG - chr4_1:68824976-68824995 None:intergenic 60.0%
CACGGAGAGCCTTCTTCTCG+GGG - chr4_1:68824996-68825015 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 68821065 68825114 68821065 ID=Msa0567490;Name=Msa0567490
chr4_1 mRNA 68821065 68825114 68821065 ID=Msa0567490-mRNA-1;Parent=Msa0567490;Name=Msa0567490-mRNA-1;_AED=0.03;_eAED=0.03;_QI=161|1|1|1|1|0.8|5|1046|369
chr4_1 exon 68821065 68821362 68821065 ID=Msa0567490-mRNA-1:exon:22788;Parent=Msa0567490-mRNA-1
chr4_1 exon 68821520 68821589 68821520 ID=Msa0567490-mRNA-1:exon:22789;Parent=Msa0567490-mRNA-1
chr4_1 exon 68822199 68823541 68822199 ID=Msa0567490-mRNA-1:exon:22790;Parent=Msa0567490-mRNA-1
chr4_1 exon 68823646 68823774 68823646 ID=Msa0567490-mRNA-1:exon:22791;Parent=Msa0567490-mRNA-1
chr4_1 exon 68824638 68825114 68824638 ID=Msa0567490-mRNA-1:exon:22792;Parent=Msa0567490-mRNA-1
chr4_1 five_prime_UTR 68821065 68821225 68821065 ID=Msa0567490-mRNA-1:five_prime_utr;Parent=Msa0567490-mRNA-1
chr4_1 CDS 68821226 68821362 68821226 ID=Msa0567490-mRNA-1:cds;Parent=Msa0567490-mRNA-1
chr4_1 CDS 68821520 68821589 68821520 ID=Msa0567490-mRNA-1:cds;Parent=Msa0567490-mRNA-1
chr4_1 CDS 68822199 68823101 68822199 ID=Msa0567490-mRNA-1:cds;Parent=Msa0567490-mRNA-1
chr4_1 three_prime_UTR 68823102 68823541 68823102 ID=Msa0567490-mRNA-1:three_prime_utr;Parent=Msa0567490-mRNA-1
chr4_1 three_prime_UTR 68823646 68823774 68823646 ID=Msa0567490-mRNA-1:three_prime_utr;Parent=Msa0567490-mRNA-1
chr4_1 three_prime_UTR 68824638 68825114 68824638 ID=Msa0567490-mRNA-1:three_prime_utr;Parent=Msa0567490-mRNA-1
Gene Sequence

>Msa0567490

ATGAAATCACTTCAAGTGGCCCAAGCACAAGACCCTGAAAGTCAGTCTGACAGAACTGAACAAGTTTCAGATAAAAGCATAGTTATTCCCAACAAACATGTCACAATAGCTGATAGCATAAAAAATGAACATTCAAGGTTCGTTGCCCCTCGAATCCCAACAGACTTTTCAATTCAAGTCCAGGAAACCACTTATAATGTGCACAAGTACCCCTTGATATCAAAATGCGGCTACATAGGACGGTCGGAAGTACAGCCTCTAATCTCAAATTCTGGGAATGTGCTCAAGCTTGAAAACTTTCCAGGTGGATGTGAAACATTTGAAACAATTCTAAAATTCTGTTATGGCCTCCCTATAGACTTCAACTCGGATAATGTAGCTGCACTAAGATGTGCATCAGAATTTCTAGAGATGACTGAAGAACTAGAAGATGGCAATCTCATTTCCAAGACTGAAGCTTTCCTCACATTTGTCGTGCTTTCTTCATGGAAAGACACTGTTACTGTTCTGAAATCTTGTGAAAATCTATCTCCATGGGCTGAAAATATCCAAATTGTTAGAAGATGCTGTGATTCTATAGCTTGGAAGGCTACTAAAAATGAGCCCGCAAGTGAGGATGCAGCATCTAATCAAGAAAGCTGGTGGTTCGATGATTTGGCCACCCTCCGCATTGATCATTTTATGCGGATAATTTCAGCAATTAGGGCAAAGGGAACCAAACCAGAGATCATTGGTAAATGTATCATGCAATATGCCAAGAGATGGCTACCAGGAATGTATGAGGAGTTAGAAGGGCTTAGAGGATATGGGCATGGAAAAAGTAATCTACAGTTCACTATATTTAGCATGAAGAAAGAAGAGAGCACTAGACATAGCAAGGAGCAAACGACTATTATTGAAAGCCTAATAAGCATAATTCCTCCTCAACAAGATGCTGTCCCATGTAAGTTCTTGTTGCAGATGCTAAAGATGGCAATGATGTATTCTGTATCATCAGCTCTCATTTCAGACCTTGAAAAGAGAGTTGGTATGGTGTTAGAAGATGCTGAGGTGAATGATCTCTTAATTCCTAGATATCAAAATGGAGGTCAAGGAAAAATGGTCAAGTGA
Protein sequence

>Msa0567490

MKSLQVAQAQDPESQSDRTEQVSDKSIVIPNKHVTIADSIKNEHSRFVAPRIPTDFSIQVQETTYNVHKYPLISKCGYIGRSEVQPLISNSGNVLKLENFPGGCETFETILKFCYGLPIDFNSDNVAALRCASEFLEMTEELEDGNLISKTEAFLTFVVLSSWKDTVTVLKSCENLSPWAENIQIVRRCCDSIAWKATKNEPASEDAASNQESWWFDDLATLRIDHFMRIISAIRAKGTKPEIIGKCIMQYAKRWLPGMYEELEGLRGYGHGKSNLQFTIFSMKKEESTRHSKEQTTIIESLISIIPPQQDAVPCKFLLQMLKMAMMYSVSSALISDLEKRVGMVLEDAEVNDLLIPRYQNGGQGKMVK*