AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0653510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0653510 MtrunA17_Chr1g0210231 78.000 50 10 1 1 49 225 274 2.06e-18 79.0
Msa0653510 MtrunA17_Chr8g0360481 68.000 50 15 1 1 49 207 256 5.31e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0653510 AT3G10420.1 74.000 50 12 1 1 49 236 285 1.46e-15 71.2
Msa0653510 AT3G10420.2 74.000 50 12 1 1 49 236 285 2.05e-15 70.9
Msa0653510 AT1G73170.2 58.491 53 21 1 1 52 92 144 4.58e-12 61.2
Msa0653510 AT1G73170.1 62.000 50 18 1 1 49 220 269 5.51e-12 60.8
Msa0653510 AT1G73170.3 62.000 50 18 1 1 49 220 269 5.51e-12 60.8

Find 31 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGTCCCTCATTTGGCTTT+GGG 0.238096 4_3:+61548600 Msa0653510:CDS
TCTAGTCCCTCATTTGGCTT+TGG 0.282579 4_3:+61548599 Msa0653510:CDS
GTGGATACATCCAATGAAAT+TGG 0.303806 4_3:+61546288 Msa0653510:CDS
ACAAAGATGATGCAAGAAAA+TGG 0.304915 4_3:+61548639 Msa0653510:CDS
GGTGGAGATGTTCTTCTTGC+AGG 0.311714 4_3:+61546312 Msa0653510:CDS
CAATGCTGCATATTCACATT+TGG 0.366625 4_3:-61546363 None:intergenic
CATCTTTGTCTCTATTCTAT+AGG 0.368076 4_3:-61548625 None:intergenic
ATCTCCACCTCCAATTTCAT+TGG 0.369281 4_3:-61546298 None:intergenic
CGTGAAGCACGTAGCGATAG+TGG 0.405578 4_3:+61546269 Msa0653510:CDS
GGTTCCTCTAGTCCCTCATT+TGG 0.423441 4_3:+61548593 Msa0653510:CDS
GTGTGAAGGATTCAACAATA+TGG 0.437580 4_3:+61548775 Msa0653510:CDS
TGTAAAGAGGATTCATGTAT+TGG 0.451962 4_3:+61548552 Msa0653510:CDS
TGGTGGCTCTTCATTGCTGC+CGG 0.475420 4_3:+61548572 Msa0653510:CDS
ACAATATGGCAAATTGCAAA+TGG 0.475777 4_3:+61548789 Msa0653510:CDS
TAGTCCCTCATTTGGCTTTG+GGG 0.481397 4_3:+61548601 Msa0653510:CDS
GATGTTCTTCTTGCAGGCAT+AGG 0.494679 4_3:+61546318 Msa0653510:CDS
AAATGAGGGACTAGAGGAAC+CGG 0.504340 4_3:-61548591 None:intergenic
GAATCCTTCACACTATCTGC+AGG 0.534749 4_3:-61548765 None:intergenic
ACATCCAATGAAATTGGAGG+TGG 0.549569 4_3:+61546294 Msa0653510:CDS
TAGGTCCCCAAAGCCAAATG+AGG 0.552454 4_3:-61548606 None:intergenic
ATACATGAATCCTCTTTACA+TGG 0.553024 4_3:-61548549 None:intergenic
AGGATTAGAACCATGTAAAG+AGG 0.573849 4_3:+61548539 Msa0653510:intron
AGCACCTGCAGATAGTGTGA+AGG 0.594876 4_3:+61548761 Msa0653510:intron
AAAGAGGATTCATGTATTGG+TGG 0.596504 4_3:+61548555 Msa0653510:CDS
GATACATCCAATGAAATTGG+AGG 0.601573 4_3:+61546291 Msa0653510:CDS
GTGAATATGCAGCATTGTGT+TGG 0.623077 4_3:+61546369 Msa0653510:CDS
TCTTCTTGCAGGCATAGGCA+AGG 0.624584 4_3:+61546323 Msa0653510:CDS
TTGCAGGCATAGGCAAGGCA+AGG 0.626398 4_3:+61546328 Msa0653510:CDS
AGGTCCCCAAAGCCAAATGA+GGG 0.631355 4_3:-61548605 None:intergenic
AAAGCCAAATGAGGGACTAG+AGG 0.635489 4_3:-61548597 None:intergenic
CAGGCATAGGCAAGGCAAGG+AGG 0.679532 4_3:+61546331 Msa0653510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCGATAAAAAAATAAAAAAT+AGG - chr4_3:61547215-61547234 None:intergenic 10.0%
!! TTAGATTAATTAACAAAAAA+AGG + chr4_3:61547307-61547326 Msa0653510:intron 10.0%
!! TTATTTAAATGAAAAAAATG+AGG + chr4_3:61548130-61548149 Msa0653510:intron 10.0%
!! TATTTAAATGAAAAAAATGA+GGG + chr4_3:61548131-61548150 Msa0653510:intron 10.0%
!!! ATAAATTTTGAACATACAAA+AGG + chr4_3:61548674-61548693 Msa0653510:intron 15.0%
!! AAGAATAGTGATTATAAATG+AGG - chr4_3:61546720-61546739 None:intergenic 20.0%
!! TTGTTAAAAGGATATTCATT+TGG - chr4_3:61546864-61546883 None:intergenic 20.0%
!!! AATACCAATCTCTATTATTT+TGG + chr4_3:61546938-61546957 Msa0653510:intron 20.0%
!! GCAATTAAATATTATCCTTT+GGG - chr4_3:61547099-61547118 None:intergenic 20.0%
!! AGCAATTAAATATTATCCTT+TGG - chr4_3:61547100-61547119 None:intergenic 20.0%
!!! TTAAGTAGCATTTTTTTAGT+AGG + chr4_3:61547159-61547178 Msa0653510:intron 20.0%
!! AATAAAAAATAGGTAAGTGA+GGG - chr4_3:61547205-61547224 None:intergenic 20.0%
!! AAATAAAAAATAGGTAAGTG+AGG - chr4_3:61547206-61547225 None:intergenic 20.0%
!! AAAGATCTACAATTACATAA+AGG - chr4_3:61547260-61547279 None:intergenic 20.0%
!! AACATAAATGAAAATACTCT+TGG + chr4_3:61547722-61547741 Msa0653510:intron 20.0%
!! AATAACAAAAATATTGAGTG+AGG + chr4_3:61547918-61547937 Msa0653510:intron 20.0%
!! TGTTGAAGTTTAAATAATAC+TGG + chr4_3:61547978-61547997 Msa0653510:intron 20.0%
!! AAGTTTAAATAATACTGGAA+TGG + chr4_3:61547983-61548002 Msa0653510:intron 20.0%
!! AGTTTAAATAATACTGGAAT+GGG + chr4_3:61547984-61548003 Msa0653510:intron 20.0%
!! ATTTCTACCTATTTAACATT+TGG + chr4_3:61548096-61548115 Msa0653510:intron 20.0%
!!! TGTTAATGAACCATTTTTTT+TGG + chr4_3:61548484-61548503 Msa0653510:intron 20.0%
!!! TTTTCTTTGTCCAAAAAAAA+TGG - chr4_3:61548497-61548516 None:intergenic 20.0%
!! TAAATACATCATATTCTGAT+TGG - chr4_3:61548742-61548761 None:intergenic 20.0%
! AGTCAAACATATCACAAAAA+GGG - chr4_3:61546441-61546460 None:intergenic 25.0%
! TTGATGTTGAGATCTAAATT+GGG - chr4_3:61546487-61546506 None:intergenic 25.0%
! TTTGATGTTGAGATCTAAAT+TGG - chr4_3:61546488-61546507 None:intergenic 25.0%
! GACTTACATAAAATGAAGTT+TGG - chr4_3:61546532-61546551 None:intergenic 25.0%
! GTGAAATATTAGTGTAAATG+AGG - chr4_3:61546791-61546810 None:intergenic 25.0%
!! TCACTAAAAAATCAGTGTTT+TGG + chr4_3:61546807-61546826 Msa0653510:intron 25.0%
! GTAGTGTTATGATTGTTAAA+AGG - chr4_3:61546876-61546895 None:intergenic 25.0%
! GAAACCAAAATAATAGAGAT+TGG - chr4_3:61546945-61546964 None:intergenic 25.0%
!!! TTTTTAGTAGGATCATTTGA+TGG + chr4_3:61547171-61547190 Msa0653510:intron 25.0%
! AGGTTCTCAAAAAGATAAAA+AGG - chr4_3:61547240-61547259 None:intergenic 25.0%
! AGAACAAATTGTAAAGTCAA+AGG + chr4_3:61547547-61547566 Msa0653510:intron 25.0%
! ACAATAACACAGGAAAAAAT+TGG - chr4_3:61547757-61547776 None:intergenic 25.0%
!!! TTTTTCCTGTGTTATTGTAT+AGG + chr4_3:61547759-61547778 Msa0653510:intron 25.0%
! GTTTAAATAATACTGGAATG+GGG + chr4_3:61547985-61548004 Msa0653510:intron 25.0%
! TTGATTCTCATGTAAATGAT+AGG + chr4_3:61548519-61548538 Msa0653510:intron 25.0%
CAGTCAAACATATCACAAAA+AGG - chr4_3:61546442-61546461 None:intergenic 30.0%
TGATGTTGAGATCTAAATTG+GGG - chr4_3:61546486-61546505 None:intergenic 30.0%
GTAAGTCAAAAAAATCACCT+TGG + chr4_3:61546545-61546564 Msa0653510:intron 30.0%
AAAATTTAGCATATGCTCCA+AGG - chr4_3:61546565-61546584 None:intergenic 30.0%
! TTTTCAATCACCATGAATGA+AGG + chr4_3:61546599-61546618 Msa0653510:intron 30.0%
TAGTAAGGTTTATGTCAAGA+AGG - chr4_3:61546647-61546666 None:intergenic 30.0%
!!! GGGAAGGTATTATTTTAGTA+AGG - chr4_3:61546662-61546681 None:intergenic 30.0%
! TTTTCAGTGATCTAGATAGA+AGG - chr4_3:61546911-61546930 None:intergenic 30.0%
TCTATCTAGATCACTGAAAA+TGG + chr4_3:61546911-61546930 Msa0653510:intron 30.0%
GCATAATATCATGATGCAAT+TGG - chr4_3:61547449-61547468 None:intergenic 30.0%
CTTTCAATCTATCTCAAGTT+GGG - chr4_3:61547475-61547494 None:intergenic 30.0%
TCTTTCAATCTATCTCAAGT+TGG - chr4_3:61547476-61547495 None:intergenic 30.0%
! GACTTTACAATTTGTTCTCA+AGG - chr4_3:61547545-61547564 None:intergenic 30.0%
TAGTCACAGCTTAAAATGTA+GGG - chr4_3:61547604-61547623 None:intergenic 30.0%
ATAGTCACAGCTTAAAATGT+AGG - chr4_3:61547605-61547624 None:intergenic 30.0%
TGAATCCTATACAATAACAC+AGG - chr4_3:61547767-61547786 None:intergenic 30.0%
TAATACAATTACACACGCAA+AGG - chr4_3:61547828-61547847 None:intergenic 30.0%
ACAAAAATATTGAGTGAGGA+CGG + chr4_3:61547922-61547941 Msa0653510:intron 30.0%
! TTTTCATCAACTTTCTGTGA+TGG + chr4_3:61548021-61548040 Msa0653510:intron 30.0%
! AACTTTCTGTGATGGAATAT+AGG + chr4_3:61548029-61548048 Msa0653510:intron 30.0%
CTTCAAGCCAAATGTTAAAT+AGG - chr4_3:61548106-61548125 None:intergenic 30.0%
ATACATGAATCCTCTTTACA+TGG - chr4_3:61548552-61548571 None:intergenic 30.0%
TGTAAAGAGGATTCATGTAT+TGG + chr4_3:61548552-61548571 Msa0653510:CDS 30.0%
CATCTTTGTCTCTATTCTAT+AGG - chr4_3:61548628-61548647 None:intergenic 30.0%
ACAAAGATGATGCAAGAAAA+TGG + chr4_3:61548639-61548658 Msa0653510:CDS 30.0%
AACATACAAAAGGAGAAGAA+AGG + chr4_3:61548684-61548703 Msa0653510:intron 30.0%
AAAGGAAAAACAACTCATGA+AGG + chr4_3:61548702-61548721 Msa0653510:intron 30.0%
ACAATATGGCAAATTGCAAA+TGG + chr4_3:61548789-61548808 Msa0653510:CDS 30.0%
GTGGATACATCCAATGAAAT+TGG + chr4_3:61546288-61546307 Msa0653510:CDS 35.0%
GATACATCCAATGAAATTGG+AGG + chr4_3:61546291-61546310 Msa0653510:CDS 35.0%
CAATGCTGCATATTCACATT+TGG - chr4_3:61546366-61546385 None:intergenic 35.0%
ATGAAGGGTTTATATAGGGA+AGG - chr4_3:61546678-61546697 None:intergenic 35.0%
GGTGATGAAGGGTTTATATA+GGG - chr4_3:61546682-61546701 None:intergenic 35.0%
! TGGTGATGAAGGGTTTATAT+AGG - chr4_3:61546683-61546702 None:intergenic 35.0%
ATGAGGAAAAGTGATGCAAA+TGG - chr4_3:61546703-61546722 None:intergenic 35.0%
!! AAAAATCAGTGTTTTGGACC+TGG + chr4_3:61546813-61546832 Msa0653510:intron 35.0%
!! AAAATCAGTGTTTTGGACCT+GGG + chr4_3:61546814-61546833 Msa0653510:intron 35.0%
! ATTTGGCATCTGTGATTTTG+AGG - chr4_3:61546847-61546866 None:intergenic 35.0%
AATTGCTCAGTTTAGTGCTT+TGG + chr4_3:61547113-61547132 Msa0653510:intron 35.0%
AAAAAATAGGTAAGTGAGGG+AGG - chr4_3:61547202-61547221 None:intergenic 35.0%
ATGATGACACATTCTCTAGA+TGG + chr4_3:61547504-61547523 Msa0653510:intron 35.0%
CAAAAATATTGAGTGAGGAC+GGG + chr4_3:61547923-61547942 Msa0653510:intron 35.0%
! AGACATTCAAGATTTTGAGC+TGG + chr4_3:61548295-61548314 Msa0653510:intron 35.0%
! GACATTCAAGATTTTGAGCT+GGG + chr4_3:61548296-61548315 Msa0653510:intron 35.0%
TCAAAGTTTCCCAATGTGTT+TGG - chr4_3:61548351-61548370 None:intergenic 35.0%
AGGATTAGAACCATGTAAAG+AGG + chr4_3:61548539-61548558 Msa0653510:intron 35.0%
AAAGAGGATTCATGTATTGG+TGG + chr4_3:61548555-61548574 Msa0653510:CDS 35.0%
GTGTGAAGGATTCAACAATA+TGG + chr4_3:61548775-61548794 Msa0653510:CDS 35.0%
ACATCCAATGAAATTGGAGG+TGG + chr4_3:61546294-61546313 Msa0653510:CDS 40.0%
ATCTCCACCTCCAATTTCAT+TGG - chr4_3:61546301-61546320 None:intergenic 40.0%
GTGAATATGCAGCATTGTGT+TGG + chr4_3:61546369-61546388 Msa0653510:CDS 40.0%
GTTGAGATCTAAATTGGGGA+AGG - chr4_3:61546482-61546501 None:intergenic 40.0%
ACAATGCACTCCTTCATTCA+TGG - chr4_3:61546612-61546631 None:intergenic 40.0%
! GTGATGCAAATGGTGATGAA+GGG - chr4_3:61546693-61546712 None:intergenic 40.0%
! AGTGATGCAAATGGTGATGA+AGG - chr4_3:61546694-61546713 None:intergenic 40.0%
ACACATACAGTGCTCAGTTT+AGG + chr4_3:61547064-61547083 Msa0653510:intron 40.0%
TTTAGGTCTCAACATCCCAA+AGG + chr4_3:61547081-61547100 Msa0653510:intron 40.0%
! TAGTAGGATCATTTGATGGC+TGG + chr4_3:61547175-61547194 Msa0653510:intron 40.0%
!! CAATCTATCTCAAGTTGGGT+AGG - chr4_3:61547471-61547490 None:intergenic 40.0%
! TAAAATGTAGGGAGGTGTGA+AGG - chr4_3:61547593-61547612 None:intergenic 40.0%
TCACAGCTTAAAATGTAGGG+AGG - chr4_3:61547601-61547620 None:intergenic 40.0%
ATGGGATTCACAATCTTGCT+TGG + chr4_3:61547686-61547705 Msa0653510:intron 40.0%
TGGGATTCACAATCTTGCTT+GGG + chr4_3:61547687-61547706 Msa0653510:intron 40.0%
GGGAGATTAGAATGCATTGA+AGG + chr4_3:61548316-61548335 Msa0653510:intron 40.0%
GAATGCATTGAAGGAGTTGT+TGG + chr4_3:61548325-61548344 Msa0653510:intron 40.0%
AGTTGTTGGCCAAACACATT+GGG + chr4_3:61548339-61548358 Msa0653510:intron 40.0%
ACATTGGGAAACTTTGACGA+AGG + chr4_3:61548354-61548373 Msa0653510:intron 40.0%
GAGTTATGTGAAAGCAAAGC+TGG - chr4_3:61548385-61548404 None:intergenic 40.0%
GATGTTCTTCTTGCAGGCAT+AGG + chr4_3:61546318-61546337 Msa0653510:CDS 45.0%
TGTTCTGCCGTCTCAAATCT+TGG + chr4_3:61547657-61547676 Msa0653510:intron 45.0%
GTTCTGCCGTCTCAAATCTT+GGG + chr4_3:61547658-61547677 Msa0653510:intron 45.0%
TCCCAACCCAAGATTTGAGA+CGG - chr4_3:61547667-61547686 None:intergenic 45.0%
!! TCTCAAATCTTGGGTTGGGA+TGG + chr4_3:61547667-61547686 Msa0653510:intron 45.0%
!! CTCAAATCTTGGGTTGGGAT+GGG + chr4_3:61547668-61547687 Msa0653510:intron 45.0%
GAGTTGTTGGCCAAACACAT+TGG + chr4_3:61548338-61548357 Msa0653510:intron 45.0%
AAATGAGGGACTAGAGGAAC+CGG - chr4_3:61548594-61548613 None:intergenic 45.0%
AAAGCCAAATGAGGGACTAG+AGG - chr4_3:61548600-61548619 None:intergenic 45.0%
! TCTAGTCCCTCATTTGGCTT+TGG + chr4_3:61548599-61548618 Msa0653510:CDS 45.0%
!! CTAGTCCCTCATTTGGCTTT+GGG + chr4_3:61548600-61548619 Msa0653510:CDS 45.0%
!! TAGTCCCTCATTTGGCTTTG+GGG + chr4_3:61548601-61548620 Msa0653510:CDS 45.0%
GAATCCTTCACACTATCTGC+AGG - chr4_3:61548768-61548787 None:intergenic 45.0%
!! AATATATTGTATTATTATAT+AGG + chr4_3:61548179-61548198 Msa0653510:intron 5.0%
GGTGGAGATGTTCTTCTTGC+AGG + chr4_3:61546312-61546331 Msa0653510:CDS 50.0%
TCTTCTTGCAGGCATAGGCA+AGG + chr4_3:61546323-61546342 Msa0653510:CDS 50.0%
TGCCGTCTCAAATCTTGGGT+TGG + chr4_3:61547662-61547681 Msa0653510:intron 50.0%
GCCGTCTCAAATCTTGGGTT+GGG + chr4_3:61547663-61547682 Msa0653510:intron 50.0%
GGTTCCTCTAGTCCCTCATT+TGG + chr4_3:61548593-61548612 Msa0653510:CDS 50.0%
AGGTCCCCAAAGCCAAATGA+GGG - chr4_3:61548608-61548627 None:intergenic 50.0%
TAGGTCCCCAAAGCCAAATG+AGG - chr4_3:61548609-61548628 None:intergenic 50.0%
AGCACCTGCAGATAGTGTGA+AGG + chr4_3:61548761-61548780 Msa0653510:intron 50.0%
CGTGAAGCACGTAGCGATAG+TGG + chr4_3:61546269-61546288 Msa0653510:CDS 55.0%
TTGCAGGCATAGGCAAGGCA+AGG + chr4_3:61546328-61546347 Msa0653510:CDS 55.0%
!! GATTTTGAGGACGTGCTCCC+AGG - chr4_3:61546834-61546853 None:intergenic 55.0%
TGGTGGCTCTTCATTGCTGC+CGG + chr4_3:61548572-61548591 Msa0653510:CDS 55.0%
CAGGCATAGGCAAGGCAAGG+AGG + chr4_3:61546331-61546350 Msa0653510:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 61546252 61548818 61546252 ID=Msa0653510;Name=Msa0653510
chr4_3 mRNA 61546252 61548818 61546252 ID=Msa0653510-mRNA-1;Parent=Msa0653510;Name=Msa0653510-mRNA-1;_AED=0.68;_eAED=0.68;_QI=0|0|0|0.33|1|1|3|0|105
chr4_3 exon 61546252 61546390 61546252 ID=Msa0653510-mRNA-1:exon:16903;Parent=Msa0653510-mRNA-1
chr4_3 exon 61548541 61548663 61548541 ID=Msa0653510-mRNA-1:exon:16904;Parent=Msa0653510-mRNA-1
chr4_3 exon 61548763 61548818 61548763 ID=Msa0653510-mRNA-1:exon:16905;Parent=Msa0653510-mRNA-1
chr4_3 CDS 61546252 61546390 61546252 ID=Msa0653510-mRNA-1:cds;Parent=Msa0653510-mRNA-1
chr4_3 CDS 61548541 61548663 61548541 ID=Msa0653510-mRNA-1:cds;Parent=Msa0653510-mRNA-1
chr4_3 CDS 61548763 61548818 61548763 ID=Msa0653510-mRNA-1:cds;Parent=Msa0653510-mRNA-1
Gene Sequence

>Msa0653510

ATGTTGGCAGATGAATTCGTGAAGCACGTAGCGATAGTGGATACATCCAATGAAATTGGAGGTGGAGATGTTCTTCTTGCAGGCATAGGCAAGGCAAGGAGGATGCAAGTTCCAAATGTGAATATGCAGCATTGTGTTGGATTAGAACCATGTAAAGAGGATTCATGTATTGGTGGCTCTTCATTGCTGCCGGTTCCTCTAGTCCCTCATTTGGCTTTGGGGACCTATAGAATAGAGACAAAGATGATGCAAGAAAATGGATCACCTGCAGATAGTGTGAAGGATTCAACAATATGGCAAATTGCAAATGGCAAATGA

Protein sequence

>Msa0653510

MLADEFVKHVAIVDTSNEIGGGDVLLAGIGKARRMQVPNVNMQHCVGLEPCKEDSCIGGSSLLPVPLVPHLALGTYRIETKMMQENGSPADSVKDSTIWQIANGK*