Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192590 | PNX58556.1 | 89.189 | 148 | 16 | 0 | 1 | 148 | 1 | 148 | 5.51e-87 | 260 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192590 | A0A2K3JX36 | 89.189 | 148 | 16 | 0 | 1 | 148 | 1 | 148 | 2.63e-87 | 260 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001540 | Msa0192590 | 0.811481 | 6.852211e-51 | -8.615850e-47 |
| Msa0012460 | Msa0192590 | 0.809093 | 2.243250e-50 | -8.615850e-47 |
| Msa0024070 | Msa0192590 | 0.807045 | 6.121535e-50 | -8.615850e-47 |
| Msa0025830 | Msa0192590 | 0.810295 | 1.237839e-50 | -8.615850e-47 |
| Msa0035090 | Msa0192590 | 0.808746 | 2.662386e-50 | -8.615850e-47 |
| Msa0044250 | Msa0192590 | 0.845366 | 4.247873e-59 | -8.615850e-47 |
| Msa0044320 | Msa0192590 | 0.800237 | 1.583360e-48 | -8.615850e-47 |
| Msa0044380 | Msa0192590 | 0.812773 | 3.581775e-51 | -8.615850e-47 |
| Msa0067820 | Msa0192590 | 0.807384 | 5.188508e-50 | -8.615850e-47 |
| Msa0073350 | Msa0192590 | 0.816262 | 6.054766e-52 | -8.615850e-47 |
| Msa0079530 | Msa0192590 | 0.806059 | 9.883521e-50 | -8.615850e-47 |
| Msa0090760 | Msa0192590 | 0.804898 | 1.731852e-49 | -8.615850e-47 |
| Msa0092660 | Msa0192590 | 0.802204 | 6.267828e-49 | -8.615850e-47 |
| Msa0104190 | Msa0192590 | 0.816168 | 6.355060e-52 | -8.615850e-47 |
| Msa0120980 | Msa0192590 | 0.803902 | 2.793081e-49 | -8.615850e-47 |
| Msa0175280 | Msa0192590 | 0.801784 | 7.648511e-49 | -8.615850e-47 |
| Msa0181010 | Msa0192590 | 0.804793 | 1.821391e-49 | -8.615850e-47 |
| Msa0183490 | Msa0192590 | 0.810769 | 9.774750e-51 | -8.615850e-47 |
| Msa0185550 | Msa0192590 | 0.800843 | 1.191697e-48 | -8.615850e-47 |
| Msa0190000 | Msa0192590 | 0.820921 | 5.313038e-53 | -8.615850e-47 |
| Msa0192520 | Msa0192590 | 0.970540 | 8.990865e-132 | -8.615850e-47 |
| Msa0192590 | Msa0206970 | 0.811121 | 8.202479e-51 | -8.615850e-47 |
| Msa0192590 | Msa0223390 | 0.805799 | 1.121439e-49 | -8.615850e-47 |
| Msa0192590 | Msa0224980 | 0.806158 | 9.420760e-50 | -8.615850e-47 |
| Msa0192590 | Msa0225030 | 0.805057 | 1.603973e-49 | -8.615850e-47 |
| Msa0192590 | Msa0235180 | 0.814547 | 1.457064e-51 | -8.615850e-47 |
| Msa0192590 | Msa0236350 | 0.820184 | 7.843144e-53 | -8.615850e-47 |
| Msa0192590 | Msa0250520 | 0.811485 | 6.839226e-51 | -8.615850e-47 |
| Msa0192590 | Msa0253410 | 0.812984 | 3.219935e-51 | -8.615850e-47 |
| Msa0192590 | Msa0258380 | 0.814209 | 1.731269e-51 | -8.615850e-47 |
| Msa0192590 | Msa0258410 | 0.817330 | 3.488178e-52 | -8.615850e-47 |
| Msa0192590 | Msa0267130 | 0.802562 | 5.290505e-49 | -8.615850e-47 |
| Msa0192590 | Msa0272680 | 0.992556 | 5.291971e-194 | -8.615850e-47 |
| Msa0192590 | Msa0274870 | 0.819379 | 1.197659e-52 | -8.615850e-47 |
| Msa0192590 | Msa0282860 | 0.804602 | 1.996982e-49 | -8.615850e-47 |
| Msa0192590 | Msa0284260 | 0.800196 | 1.613965e-48 | -8.615850e-47 |
| Msa0192590 | Msa0285740 | 0.810218 | 1.285714e-50 | -8.615850e-47 |
| Msa0192590 | Msa0288360 | 0.800827 | 1.200817e-48 | -8.615850e-47 |
| Msa0192590 | Msa0302660 | 0.815240 | 1.022959e-51 | -8.615850e-47 |
| Msa0192590 | Msa0307750 | 0.806573 | 7.704913e-50 | -8.615850e-47 |
| Msa0192590 | Msa0313800 | 0.800637 | 1.312835e-48 | -8.615850e-47 |
| Msa0192590 | Msa0327900 | 0.801301 | 9.606442e-49 | -8.615850e-47 |
| Msa0192590 | Msa0335740 | 0.817928 | 2.557330e-52 | -8.615850e-47 |
| Msa0192590 | Msa0340440 | 0.800614 | 1.326780e-48 | -8.615850e-47 |
| Msa0192590 | Msa0343920 | 0.800605 | 1.332461e-48 | -8.615850e-47 |
| Msa0192590 | Msa0347060 | 0.805741 | 1.152975e-49 | -8.615850e-47 |
| Msa0192590 | Msa0353890 | 0.800007 | 1.763439e-48 | -8.615850e-47 |
| Msa0192590 | Msa0354490 | 0.815022 | 1.143712e-51 | -8.615850e-47 |
| Msa0192590 | Msa0367370 | 0.822829 | 1.920769e-53 | -8.615850e-47 |
| Msa0192590 | Msa0368430 | 0.802017 | 6.850032e-49 | -8.615850e-47 |
| Msa0192590 | Msa0370180 | 0.834587 | 2.749308e-56 | -8.615850e-47 |
| Msa0192590 | Msa0371050 | 0.807916 | 4.000192e-50 | -8.615850e-47 |
| Msa0192590 | Msa0371870 | 0.800621 | 1.322365e-48 | -8.615850e-47 |
| Msa0192590 | Msa0376760 | 0.810998 | 8.721030e-51 | -8.615850e-47 |
| Msa0192590 | Msa0379090 | 0.823470 | 1.361657e-53 | -8.615850e-47 |
| Msa0192590 | Msa0379110 | 0.815182 | 1.053864e-51 | -8.615850e-47 |
| Msa0192590 | Msa0379900 | 0.807211 | 5.647389e-50 | -8.615850e-47 |
| Msa0192590 | Msa0383570 | 0.814176 | 1.760441e-51 | -8.615850e-47 |
| Msa0192590 | Msa0387120 | 0.804155 | 2.473597e-49 | -8.615850e-47 |
| Msa0192590 | Msa0396970 | 0.804731 | 1.876123e-49 | -8.615850e-47 |
| Msa0192590 | Msa0397000 | 0.801363 | 9.329911e-49 | -8.615850e-47 |
| Msa0192590 | Msa0401370 | 0.810023 | 1.416236e-50 | -8.615850e-47 |
| Msa0192590 | Msa0415050 | 0.803182 | 3.939208e-49 | -8.615850e-47 |
| Msa0192590 | Msa0415460 | 0.818324 | 2.080130e-52 | -8.615850e-47 |
| Msa0192590 | Msa0415930 | 0.807264 | 5.502813e-50 | -8.615850e-47 |
| Msa0192590 | Msa0421790 | 0.804299 | 2.309478e-49 | -8.615850e-47 |
| Msa0192590 | Msa0426480 | 0.817443 | 3.288406e-52 | -8.615850e-47 |
| Msa0192590 | Msa0434630 | 0.813943 | 1.981239e-51 | -8.615850e-47 |
| Msa0192590 | Msa0447820 | 0.820480 | 6.709772e-53 | -8.615850e-47 |
| Msa0192590 | Msa0450780 | 0.815461 | 9.138437e-52 | -8.615850e-47 |
| Msa0192590 | Msa0450860 | 0.800843 | 1.191355e-48 | -8.615850e-47 |
| Msa0192590 | Msa0457750 | 0.803827 | 2.894515e-49 | -8.615850e-47 |
| Msa0192590 | Msa0466070 | 0.800360 | 1.495206e-48 | -8.615850e-47 |
| Msa0192590 | Msa0466340 | 0.800286 | 1.547914e-48 | -8.615850e-47 |
| Msa0192590 | Msa0467890 | 0.814398 | 1.572062e-51 | -8.615850e-47 |
| Msa0192590 | Msa0467970 | 0.807590 | 4.692271e-50 | -8.615850e-47 |
| Msa0192590 | Msa0471700 | 0.812728 | 3.664090e-51 | -8.615850e-47 |
| Msa0192590 | Msa0473010 | 0.800802 | 1.214574e-48 | -8.615850e-47 |
| Msa0192590 | Msa0476150 | 0.801921 | 7.166944e-49 | -8.615850e-47 |
| Msa0192590 | Msa0480150 | 0.811119 | 8.211201e-51 | -8.615850e-47 |
| Msa0192590 | Msa0485350 | 0.811126 | 8.180056e-51 | -8.615850e-47 |
| Msa0192590 | Msa0492000 | 0.803068 | 4.158529e-49 | -8.615850e-47 |
| Msa0192590 | Msa0524020 | 0.808806 | 2.583926e-50 | -8.615850e-47 |
| Msa0192590 | Msa0526130 | 0.805170 | 1.519185e-49 | -8.615850e-47 |
| Msa0192590 | Msa0528870 | 0.806233 | 9.086516e-50 | -8.615850e-47 |
| Msa0192590 | Msa0553350 | 0.835682 | 1.455910e-56 | -8.615850e-47 |
| Msa0192590 | Msa0555940 | 0.813113 | 3.016985e-51 | -8.615850e-47 |
| Msa0192590 | Msa0556960 | 0.802251 | 6.130962e-49 | -8.615850e-47 |
| Msa0192590 | Msa0579610 | 0.805405 | 1.356501e-49 | -8.615850e-47 |
| Msa0192590 | Msa0583810 | 0.819456 | 1.150643e-52 | -8.615850e-47 |
| Msa0192590 | Msa0612150 | 0.800233 | 1.586889e-48 | -8.615850e-47 |
| Msa0192590 | Msa0612730 | 0.807281 | 5.458468e-50 | -8.615850e-47 |
| Msa0192590 | Msa0619060 | 0.805210 | 1.489852e-49 | -8.615850e-47 |
| Msa0192590 | Msa0619170 | 0.803027 | 4.239591e-49 | -8.615850e-47 |
| Msa0192590 | Msa0647710 | 0.848494 | 5.912428e-60 | -8.615850e-47 |
| Msa0192590 | Msa0652360 | 0.827843 | 1.250868e-54 | -8.615850e-47 |
| Msa0192590 | Msa0690530 | 0.837383 | 5.369900e-57 | -8.615850e-47 |
| Msa0192590 | Msa0695930 | 0.804894 | 1.735016e-49 | -8.615850e-47 |
| Msa0192590 | Msa0703760 | 0.803207 | 3.892286e-49 | -8.615850e-47 |
| Msa0192590 | Msa0704570 | 0.813318 | 2.720030e-51 | -8.615850e-47 |
| Msa0192590 | Msa0720380 | 0.811655 | 6.279363e-51 | -8.615850e-47 |
| Msa0192590 | Msa0732100 | 0.800857 | 1.183566e-48 | -8.615850e-47 |
| Msa0192590 | Msa0760950 | 0.802158 | 6.407488e-49 | -8.615850e-47 |
| Msa0192590 | Msa0790180 | 0.807129 | 5.877478e-50 | -8.615850e-47 |
| Msa0192590 | Msa0803220 | 0.813675 | 2.269718e-51 | -8.615850e-47 |
| Msa0192590 | Msa0814760 | 0.832678 | 8.244653e-56 | -8.615850e-47 |
| Msa0192590 | Msa0851870 | 0.831925 | 1.266221e-55 | -8.615850e-47 |
| Msa0192590 | Msa0853920 | 0.802135 | 6.477690e-49 | -8.615850e-47 |
| Msa0192590 | Msa0869090 | 0.803086 | 4.121937e-49 | -8.615850e-47 |
| Msa0192590 | Msa0881050 | 0.800332 | 1.514551e-48 | -8.615850e-47 |
| Msa0192590 | Msa0893390 | 0.822200 | 2.690079e-53 | -8.615850e-47 |
| Msa0192590 | Msa0901140 | 0.812176 | 4.835977e-51 | -8.615850e-47 |
| Msa0192590 | Msa0907890 | 0.810055 | 1.394034e-50 | -8.615850e-47 |
| Msa0192590 | Msa0946650 | 0.826617 | 2.459231e-54 | -8.615850e-47 |
| Msa0192590 | Msa0968010 | 0.816637 | 4.990798e-52 | -8.615850e-47 |
| Msa0192590 | Msa0985570 | 0.803637 | 3.170538e-49 | -8.615850e-47 |
| Msa0192590 | Msa1022310 | 0.816551 | 5.216238e-52 | -8.615850e-47 |
| Msa0192590 | Msa1029320 | 0.802424 | 5.646500e-49 | -8.615850e-47 |
| Msa0192590 | Msa1032970 | 0.813416 | 2.588509e-51 | -8.615850e-47 |
| Msa0192590 | Msa1054890 | 0.803761 | 2.986943e-49 | -8.615850e-47 |
| Msa0192590 | Msa1067430 | 0.809304 | 2.021399e-50 | -8.615850e-47 |
| Msa0192590 | Msa1080620 | 0.808249 | 3.399000e-50 | -8.615850e-47 |
| Msa0192590 | Msa1080690 | 0.810568 | 1.080534e-50 | -8.615850e-47 |
| Msa0192590 | Msa1081850 | 0.811007 | 8.684477e-51 | -8.615850e-47 |
| Msa0192590 | Msa1084150 | 0.801911 | 7.200449e-49 | -8.615850e-47 |
| Msa0192590 | Msa1090230 | 0.819050 | 1.423224e-52 | -8.615850e-47 |
| Msa0192590 | Msa1116010 | 0.808718 | 2.698086e-50 | -8.615850e-47 |
| Msa0192590 | Msa1117690 | 0.810582 | 1.073085e-50 | -8.615850e-47 |
| Msa0192590 | Msa1121630 | 0.826535 | 2.571631e-54 | -8.615850e-47 |
| Msa0192590 | Msa1126310 | 0.801519 | 8.664985e-49 | -8.615850e-47 |
| Msa0192590 | Msa1127510 | 0.800175 | 1.629986e-48 | -8.615850e-47 |
| Msa0192590 | Msa1149760 | 0.810744 | 9.898036e-51 | -8.615850e-47 |
| Msa0192590 | Msa1150170 | 0.800024 | 1.749505e-48 | -8.615850e-47 |
| Msa0192590 | Msa1154130 | 0.800200 | 1.610997e-48 | -8.615850e-47 |
| Msa0192590 | Msa1156210 | 0.835229 | 1.895328e-56 | -8.615850e-47 |
| Msa0192590 | Msa1162470 | 0.825570 | 4.361918e-54 | -8.615850e-47 |
| Msa0192590 | Msa1183610 | 0.805259 | 1.455047e-49 | -8.615850e-47 |
| Msa0192590 | Msa1183730 | 0.823175 | 1.595988e-53 | -8.615850e-47 |
| Msa0192590 | Msa1183850 | 0.804232 | 2.384866e-49 | -8.615850e-47 |
| Msa0192590 | Msa1192150 | 0.806024 | 1.005661e-49 | -8.615850e-47 |
| Msa0192590 | Msa1206440 | 0.807286 | 5.445018e-50 | -8.615850e-47 |
| Msa0192590 | Msa1213540 | 0.800558 | 1.362040e-48 | -8.615850e-47 |
| Msa0192590 | Msa1214220 | 0.802729 | 4.886633e-49 | -8.615850e-47 |
| Msa0192590 | Msa1222390 | 0.800795 | 1.218911e-48 | -8.615850e-47 |
| Msa0192590 | Msa1228950 | 0.808652 | 2.787785e-50 | -8.615850e-47 |
| Msa0192590 | Msa1234960 | 0.827060 | 1.926937e-54 | -8.615850e-47 |
| Msa0192590 | Msa1271490 | 0.800703 | 1.272733e-48 | -8.615850e-47 |
| Msa0192590 | Msa1274740 | 0.802032 | 6.800900e-49 | -8.615850e-47 |
| Msa0192590 | Msa1302750 | 0.804995 | 1.652584e-49 | -8.615850e-47 |
| Msa0192590 | Msa1322660 | 0.801815 | 7.536003e-49 | -8.615850e-47 |
| Msa0192590 | Msa1336320 | 0.812669 | 3.774144e-51 | -8.615850e-47 |
| Msa0192590 | Msa1348770 | 0.815846 | 7.500341e-52 | -8.615850e-47 |
| Msa0192590 | Msa1351370 | 0.800675 | 1.289670e-48 | -8.615850e-47 |
| Msa0192590 | Msa1356200 | 0.800768 | 1.234213e-48 | -8.615850e-47 |
| Msa0192590 | Msa1356680 | 0.816829 | 4.520602e-52 | -8.615850e-47 |
| Msa0192590 | Msa1367810 | 0.814167 | 1.768040e-51 | -8.615850e-47 |
| Msa0192590 | Msa1373500 | 0.815452 | 9.176856e-52 | -8.615850e-47 |
| Msa0192590 | Msa1374810 | 0.802714 | 4.920552e-49 | -8.615850e-47 |
| Msa0192590 | Msa1374850 | 0.810594 | 1.066828e-50 | -8.615850e-47 |
| Msa0192590 | Msa1376560 | 0.803542 | 3.317903e-49 | -8.615850e-47 |
| Msa0192590 | Msa1393200 | 0.811675 | 6.219196e-51 | -8.615850e-47 |
| Msa0192590 | Msa1406550 | 0.806719 | 7.174802e-50 | -8.615850e-47 |
| Msa0192590 | Msa1415250 | 0.811957 | 5.397062e-51 | -8.615850e-47 |
| Msa0192590 | Msa1422490 | 0.811087 | 8.341474e-51 | -8.615850e-47 |
| Msa0192590 | Msa1424240 | 0.824969 | 6.049446e-54 | -8.615850e-47 |
| Msa0192590 | Msa1430940 | 0.806359 | 8.547176e-50 | -8.615850e-47 |
| Msa0192590 | Msa1439800 | 0.813220 | 2.857397e-51 | -8.615850e-47 |
| Msa0192590 | Msa1444750 | 0.805916 | 1.059216e-49 | -8.615850e-47 |
| Msa0192590 | Msa1449320 | 0.807591 | 4.689372e-50 | -8.615850e-47 |
| Msa0192590 | Msa1455120 | 0.819045 | 1.427097e-52 | -8.615850e-47 |
| Msa0192590 | Msa1458330 | 0.802499 | 5.450805e-49 | -8.615850e-47 |
| Msa0192590 | Msa1460650 | 0.809193 | 2.135424e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192590 | MtrunA17_Chr2g0282711 | 95.270 | 148 | 7 | 0 | 1 | 148 | 3 | 150 | 8.00e-85 | 245 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0192590 | AT2G33855.1 | 55.944 | 143 | 61 | 1 | 1 | 143 | 1 | 141 | 7.64e-48 | 151 |
Find 58 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGAGGGTTGGTGTGGTTGT+GGG | 0.269536 | 2_1:+8380188 | None:intergenic |
| GGTTTGTAAGGATGTGTGTT+AGG | 0.271091 | 2_1:+8380210 | None:intergenic |
| GTGGAGGGTTGGTGTGGTTG+TGG | 0.275633 | 2_1:+8380187 | None:intergenic |
| GTGTGGTTGTGGGGTTTGTA+AGG | 0.288036 | 2_1:+8380198 | None:intergenic |
| TATCAATCAAGATAACTAAA+TGG | 0.290563 | 2_1:+8379819 | None:intergenic |
| TGGGTATTCCTAAAACTTCT+TGG | 0.291474 | 2_1:-8380037 | Msa0192590:CDS |
| GGTAATAAAATAGGTATAAG+AGG | 0.312742 | 2_1:+8379876 | None:intergenic |
| AGTGACAACAACAAAATATT+AGG | 0.321966 | 2_1:+8379855 | None:intergenic |
| TGTTGTTGTCACTTATGATA+TGG | 0.343100 | 2_1:-8379845 | Msa0192590:CDS |
| AAAATATTAGGTAATAAAAT+AGG | 0.344419 | 2_1:+8379867 | None:intergenic |
| AATTATCATTCTTGCTCAAA+AGG | 0.344967 | 2_1:-8380070 | Msa0192590:CDS |
| AAATCCCCTGCTACCTCAAA+TGG | 0.355642 | 2_1:-8380142 | Msa0192590:CDS |
| GAATCAATGGTGGAGGTTGC+TGG | 0.357663 | 2_1:+8379997 | None:intergenic |
| TAAGGATGTGTGTTAGGGCT+TGG | 0.394935 | 2_1:+8380216 | None:intergenic |
| GTTTGTAAGGATGTGTGTTA+GGG | 0.413826 | 2_1:+8380211 | None:intergenic |
| AACAAAGGAAGAGTTATGTT+AGG | 0.432276 | 2_1:-8379938 | Msa0192590:CDS |
| GGAAGAGTTATGTTAGGAAA+AGG | 0.438245 | 2_1:-8379932 | Msa0192590:CDS |
| GATGGATTGGAGCAGAGGAT+TGG | 0.459996 | 2_1:+8380110 | None:intergenic |
| CTCAAAAGGGTCTTCACAAT+GGG | 0.476531 | 2_1:-8380056 | Msa0192590:CDS |
| GAAGAGATGGATTGGAGCAG+AGG | 0.480756 | 2_1:+8380105 | None:intergenic |
| GGAGGGTTGGTGTGGTTGTG+GGG | 0.493337 | 2_1:+8380189 | None:intergenic |
| AGGGTTAACATTTGTGCCAA+GGG | 0.493789 | 2_1:+8379896 | None:intergenic |
| GGATTGGAGCAGAGGATTGG+TGG | 0.495688 | 2_1:+8380113 | None:intergenic |
| ATTATCATTCTTGCTCAAAA+GGG | 0.496190 | 2_1:-8380069 | Msa0192590:CDS |
| GTAGTCCCATTTGAGGTAGC+AGG | 0.497802 | 2_1:+8380136 | None:intergenic |
| ACTCCTTCAGCAAGAATCAA+TGG | 0.503351 | 2_1:+8379984 | None:intergenic |
| AGGAGTGGAGGAAGCACGGT+GGG | 0.505781 | 2_1:-8379967 | Msa0192590:CDS |
| AATGGTGGAGGTTGCTGGAA+TGG | 0.508912 | 2_1:+8380002 | None:intergenic |
| AATCCCCTGCTACCTCAAAT+GGG | 0.518789 | 2_1:-8380141 | Msa0192590:CDS |
| ATGTTAGGAAAAGGCTTGCA+AGG | 0.547320 | 2_1:-8379923 | Msa0192590:CDS |
| GTATTCCTAAAACTTCTTGG+AGG | 0.547416 | 2_1:-8380034 | Msa0192590:CDS |
| GAGGGTTAACATTTGTGCCA+AGG | 0.547578 | 2_1:+8379895 | None:intergenic |
| GATTGGAGCAGAGGATTGGT+GGG | 0.557001 | 2_1:+8380114 | None:intergenic |
| GTAATAAAATAGGTATAAGA+GGG | 0.558203 | 2_1:+8379877 | None:intergenic |
| GCTCAAAAGGGTCTTCACAA+TGG | 0.561078 | 2_1:-8380057 | Msa0192590:CDS |
| TAGTCCCATTTGAGGTAGCA+GGG | 0.566660 | 2_1:+8380137 | None:intergenic |
| CCACCATTGATTCTTGCTGA+AGG | 0.574245 | 2_1:-8379987 | Msa0192590:CDS |
| ATTGATTCTTGCTGAAGGAG+TGG | 0.575091 | 2_1:-8379982 | Msa0192590:CDS |
| CTGAAGGAGTGGAGGAAGCA+CGG | 0.592911 | 2_1:-8379971 | Msa0192590:CDS |
| TTGAGAGTGGAGGGTTGGTG+TGG | 0.593756 | 2_1:+8380181 | None:intergenic |
| AAGAATTGAGAGTGGAGGGT+TGG | 0.597424 | 2_1:+8380176 | None:intergenic |
| TCAGCAAGAATCAATGGTGG+AGG | 0.602784 | 2_1:+8379990 | None:intergenic |
| TTGGTGGGTAGTCCCATTTG+AGG | 0.608890 | 2_1:+8380129 | None:intergenic |
| GAGGTAGCAGGGGATTTGTG+TGG | 0.609669 | 2_1:+8380148 | None:intergenic |
| AGGCTTGCAAGGAAACCCCT+TGG | 0.615306 | 2_1:-8379912 | Msa0192590:CDS |
| GATTCTTGCTGAAGGAGTGG+AGG | 0.617592 | 2_1:-8379979 | Msa0192590:CDS |
| AGGTTGCTGGAATGGACACT+TGG | 0.621740 | 2_1:+8380010 | None:intergenic |
| CTATGAAGAATTGAGAGTGG+AGG | 0.622479 | 2_1:+8380171 | None:intergenic |
| AGTCCCATTTGAGGTAGCAG+GGG | 0.633905 | 2_1:+8380138 | None:intergenic |
| GGAGTGGAGGAAGCACGGTG+GGG | 0.635320 | 2_1:-8379966 | Msa0192590:CDS |
| TGGCTATGAAGAATTGAGAG+TGG | 0.639401 | 2_1:+8380168 | None:intergenic |
| TATGAAGAATTGAGAGTGGA+GGG | 0.652100 | 2_1:+8380172 | None:intergenic |
| AAGGAGTGGAGGAAGCACGG+TGG | 0.655059 | 2_1:-8379968 | Msa0192590:CDS |
| CACGGTGGGGAGTTGAACAA+AGG | 0.655259 | 2_1:-8379953 | Msa0192590:CDS |
| GGGTTAACATTTGTGCCAAG+GGG | 0.655878 | 2_1:+8379897 | None:intergenic |
| TGAGCATGGAAGATACAACA+TGG | 0.667356 | 2_1:-8380250 | None:intergenic |
| CCTTCAGCAAGAATCAATGG+TGG | 0.683493 | 2_1:+8379987 | None:intergenic |
| GAGCATGGAAGATACAACAT+GGG | 0.707943 | 2_1:-8380249 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATTAGGTAATAAAAT+AGG | + | chr2_1:8380202-8380221 | None:intergenic | 10.0% |
| !! | GTAATAAAATAGGTATAAGA+GGG | + | chr2_1:8380192-8380211 | None:intergenic | 20.0% |
| ! | AATTATCATTCTTGCTCAAA+AGG | - | chr2_1:8379996-8380015 | Msa0192590:CDS | 25.0% |
| ! | ATTATCATTCTTGCTCAAAA+GGG | - | chr2_1:8379997-8380016 | Msa0192590:CDS | 25.0% |
| ! | GGTAATAAAATAGGTATAAG+AGG | + | chr2_1:8380193-8380212 | None:intergenic | 25.0% |
| ! | AGTGACAACAACAAAATATT+AGG | + | chr2_1:8380214-8380233 | None:intergenic | 25.0% |
| !!! | TTTTTTGAGAAGAGATGGAT+TGG | + | chr2_1:8379972-8379991 | None:intergenic | 30.0% |
| ATCCATCTCTTCTCAAAAAA+TGG | - | chr2_1:8379972-8379991 | Msa0192590:CDS | 30.0% | |
| TCCATCTCTTCTCAAAAAAT+GGG | - | chr2_1:8379973-8379992 | Msa0192590:CDS | 30.0% | |
| AACAAAGGAAGAGTTATGTT+AGG | - | chr2_1:8380128-8380147 | Msa0192590:CDS | 30.0% | |
| ! | TGTTGTTGTCACTTATGATA+TGG | - | chr2_1:8380221-8380240 | Msa0192590:CDS | 30.0% |
| GTTTGTAAGGATGTGTGTTA+GGG | + | chr2_1:8379858-8379877 | None:intergenic | 35.0% | |
| TATGAAGAATTGAGAGTGGA+GGG | + | chr2_1:8379897-8379916 | None:intergenic | 35.0% | |
| !!! | TCCCATTTTTTGAGAAGAGA+TGG | + | chr2_1:8379977-8379996 | None:intergenic | 35.0% |
| TGGGTATTCCTAAAACTTCT+TGG | - | chr2_1:8380029-8380048 | Msa0192590:CDS | 35.0% | |
| GTATTCCTAAAACTTCTTGG+AGG | - | chr2_1:8380032-8380051 | Msa0192590:CDS | 35.0% | |
| GGAAGAGTTATGTTAGGAAA+AGG | - | chr2_1:8380134-8380153 | Msa0192590:CDS | 35.0% | |
| GGTTTGTAAGGATGTGTGTT+AGG | + | chr2_1:8379859-8379878 | None:intergenic | 40.0% | |
| CTATGAAGAATTGAGAGTGG+AGG | + | chr2_1:8379898-8379917 | None:intergenic | 40.0% | |
| TGGCTATGAAGAATTGAGAG+TGG | + | chr2_1:8379901-8379920 | None:intergenic | 40.0% | |
| CTCAAAAGGGTCTTCACAAT+GGG | - | chr2_1:8380010-8380029 | Msa0192590:CDS | 40.0% | |
| ! | TTGGACCTCCAAGAAGTTTT+AGG | + | chr2_1:8380040-8380059 | None:intergenic | 40.0% |
| ACTCCTTCAGCAAGAATCAA+TGG | + | chr2_1:8380085-8380104 | None:intergenic | 40.0% | |
| ! | ATTGATTCTTGCTGAAGGAG+TGG | - | chr2_1:8380084-8380103 | Msa0192590:CDS | 40.0% |
| ATGTTAGGAAAAGGCTTGCA+AGG | - | chr2_1:8380143-8380162 | Msa0192590:CDS | 40.0% | |
| AGGGTTAACATTTGTGCCAA+GGG | + | chr2_1:8380173-8380192 | None:intergenic | 40.0% | |
| TAAGGATGTGTGTTAGGGCT+TGG | + | chr2_1:8379853-8379872 | None:intergenic | 45.0% | |
| AAGAATTGAGAGTGGAGGGT+TGG | + | chr2_1:8379893-8379912 | None:intergenic | 45.0% | |
| AAATCCCCTGCTACCTCAAA+TGG | - | chr2_1:8379924-8379943 | Msa0192590:CDS | 45.0% | |
| AATCCCCTGCTACCTCAAAT+GGG | - | chr2_1:8379925-8379944 | Msa0192590:CDS | 45.0% | |
| ! | TAGTCCCATTTGAGGTAGCA+GGG | + | chr2_1:8379932-8379951 | None:intergenic | 45.0% |
| GCTCAAAAGGGTCTTCACAA+TGG | - | chr2_1:8380009-8380028 | Msa0192590:CDS | 45.0% | |
| TCAGCAAGAATCAATGGTGG+AGG | + | chr2_1:8380079-8380098 | None:intergenic | 45.0% | |
| CCTTCAGCAAGAATCAATGG+TGG | + | chr2_1:8380082-8380101 | None:intergenic | 45.0% | |
| !! | CCACCATTGATTCTTGCTGA+AGG | - | chr2_1:8380079-8380098 | Msa0192590:CDS | 45.0% |
| GGGTTAACATTTGTGCCAAG+GGG | + | chr2_1:8380172-8380191 | None:intergenic | 45.0% | |
| GAGGGTTAACATTTGTGCCA+AGG | + | chr2_1:8380174-8380193 | None:intergenic | 45.0% | |
| GTGTGGTTGTGGGGTTTGTA+AGG | + | chr2_1:8379871-8379890 | None:intergenic | 50.0% | |
| ! | AGTCCCATTTGAGGTAGCAG+GGG | + | chr2_1:8379931-8379950 | None:intergenic | 50.0% |
| ! | GTAGTCCCATTTGAGGTAGC+AGG | + | chr2_1:8379933-8379952 | None:intergenic | 50.0% |
| ! | TTGGTGGGTAGTCCCATTTG+AGG | + | chr2_1:8379940-8379959 | None:intergenic | 50.0% |
| ! | GATTGGAGCAGAGGATTGGT+GGG | + | chr2_1:8379955-8379974 | None:intergenic | 50.0% |
| !! | GATGGATTGGAGCAGAGGAT+TGG | + | chr2_1:8379959-8379978 | None:intergenic | 50.0% |
| !! | GAAGAGATGGATTGGAGCAG+AGG | + | chr2_1:8379964-8379983 | None:intergenic | 50.0% |
| AGGTTGCTGGAATGGACACT+TGG | + | chr2_1:8380059-8380078 | None:intergenic | 50.0% | |
| ! | AATGGTGGAGGTTGCTGGAA+TGG | + | chr2_1:8380067-8380086 | None:intergenic | 50.0% |
| ! | GAATCAATGGTGGAGGTTGC+TGG | + | chr2_1:8380072-8380091 | None:intergenic | 50.0% |
| GATTCTTGCTGAAGGAGTGG+AGG | - | chr2_1:8380087-8380106 | Msa0192590:CDS | 50.0% | |
| !! | TGGAGGGTTGGTGTGGTTGT+GGG | + | chr2_1:8379881-8379900 | None:intergenic | 55.0% |
| !! | TTGAGAGTGGAGGGTTGGTG+TGG | + | chr2_1:8379888-8379907 | None:intergenic | 55.0% |
| GAGGTAGCAGGGGATTTGTG+TGG | + | chr2_1:8379921-8379940 | None:intergenic | 55.0% | |
| GGATTGGAGCAGAGGATTGG+TGG | + | chr2_1:8379956-8379975 | None:intergenic | 55.0% | |
| CTGAAGGAGTGGAGGAAGCA+CGG | - | chr2_1:8380095-8380114 | Msa0192590:CDS | 55.0% | |
| CACGGTGGGGAGTTGAACAA+AGG | - | chr2_1:8380113-8380132 | Msa0192590:CDS | 55.0% | |
| AGGCTTGCAAGGAAACCCCT+TGG | - | chr2_1:8380154-8380173 | Msa0192590:CDS | 55.0% | |
| !! | GGAGGGTTGGTGTGGTTGTG+GGG | + | chr2_1:8379880-8379899 | None:intergenic | 60.0% |
| !! | GTGGAGGGTTGGTGTGGTTG+TGG | + | chr2_1:8379882-8379901 | None:intergenic | 60.0% |
| ! | AAGGAGTGGAGGAAGCACGG+TGG | - | chr2_1:8380098-8380117 | Msa0192590:CDS | 60.0% |
| ! | AGGAGTGGAGGAAGCACGGT+GGG | - | chr2_1:8380099-8380118 | Msa0192590:CDS | 60.0% |
| ! | GGAGTGGAGGAAGCACGGTG+GGG | - | chr2_1:8380100-8380119 | Msa0192590:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 8379821 | 8380267 | 8379821 | ID=Msa0192590;Name=Msa0192590 |
| chr2_1 | mRNA | 8379821 | 8380267 | 8379821 | ID=Msa0192590-mRNA-1;Parent=Msa0192590;Name=Msa0192590-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|148 |
| chr2_1 | exon | 8379821 | 8380267 | 8379821 | ID=Msa0192590-mRNA-1:exon:3840;Parent=Msa0192590-mRNA-1 |
| chr2_1 | CDS | 8379821 | 8380267 | 8379821 | ID=Msa0192590-mRNA-1:cds;Parent=Msa0192590-mRNA-1 |
| Gene Sequence |
| Protein sequence |
