AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0272680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0272680 MtrunA17_Chr2g0282711 95.270 148 7 0 1 148 3 150 8.00e-85 245
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0272680 AT2G33855.1 55.944 143 61 1 1 143 1 141 7.64e-48 151

Find 58 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGGGTTGGTGTGGTTGT+GGG 0.269536 2_3:+4210310 None:intergenic
GGTTTGTAAGGATGTGTGTT+AGG 0.271091 2_3:+4210332 None:intergenic
GTGGAGGGTTGGTGTGGTTG+TGG 0.275633 2_3:+4210309 None:intergenic
GTGTGGTTGTGGGGTTTGTA+AGG 0.288036 2_3:+4210320 None:intergenic
TATCAATCAAGATAACTAAA+TGG 0.290563 2_3:+4209941 None:intergenic
TGGGTATTCCTAAAACTTCT+TGG 0.291474 2_3:-4210159 Msa0272680:CDS
GGTAATAAAATAGGTATAAG+AGG 0.312742 2_3:+4209998 None:intergenic
AGTGACAACAACAAAATATT+AGG 0.321966 2_3:+4209977 None:intergenic
TGTTGTTGTCACTTATGATA+TGG 0.343100 2_3:-4209967 Msa0272680:CDS
AAAATATTAGGTAATAAAAT+AGG 0.344419 2_3:+4209989 None:intergenic
AATTATCATTCTTGCTCAAA+AGG 0.344967 2_3:-4210192 Msa0272680:CDS
AAATCCCCTGCTACCTCAAA+TGG 0.355642 2_3:-4210264 Msa0272680:CDS
GAATCAATGGTGGAGGTTGC+TGG 0.357663 2_3:+4210119 None:intergenic
TAAGGATGTGTGTTAGGGCT+TGG 0.394935 2_3:+4210338 None:intergenic
GTTTGTAAGGATGTGTGTTA+GGG 0.413826 2_3:+4210333 None:intergenic
AACAAAGGAAGAGTTATGTT+AGG 0.432276 2_3:-4210060 Msa0272680:CDS
GGAAGAGTTATGTTAGGAAA+AGG 0.438245 2_3:-4210054 Msa0272680:CDS
GATGGATTGGAGCAGAGGAT+TGG 0.459996 2_3:+4210232 None:intergenic
CTCAAAAGGGTCTTCACAAT+GGG 0.476531 2_3:-4210178 Msa0272680:CDS
GAAGAGATGGATTGGAGCAG+AGG 0.480756 2_3:+4210227 None:intergenic
GGAGGGTTGGTGTGGTTGTG+GGG 0.493337 2_3:+4210311 None:intergenic
AGGGTTAACATTTGTGCCAA+GGG 0.493789 2_3:+4210018 None:intergenic
GGATTGGAGCAGAGGATTGG+TGG 0.495688 2_3:+4210235 None:intergenic
ATTATCATTCTTGCTCAAAA+GGG 0.496190 2_3:-4210191 Msa0272680:CDS
GTAGTCCCATTTGAGGTAGC+AGG 0.497802 2_3:+4210258 None:intergenic
ACTCCTTCAGCAAGAATCAA+TGG 0.503351 2_3:+4210106 None:intergenic
AGGAGTGGAGGAAGCACGGT+GGG 0.505781 2_3:-4210089 Msa0272680:CDS
AATGGTGGAGGTTGCTGGAA+TGG 0.508912 2_3:+4210124 None:intergenic
AATCCCCTGCTACCTCAAAT+GGG 0.518789 2_3:-4210263 Msa0272680:CDS
ATGTTAGGAAAAGGCTTGCA+AGG 0.547320 2_3:-4210045 Msa0272680:CDS
GTATTCCTAAAACTTCTTGG+AGG 0.547416 2_3:-4210156 Msa0272680:CDS
GAGGGTTAACATTTGTGCCA+AGG 0.547578 2_3:+4210017 None:intergenic
GATTGGAGCAGAGGATTGGT+GGG 0.557001 2_3:+4210236 None:intergenic
GTAATAAAATAGGTATAAGA+GGG 0.558203 2_3:+4209999 None:intergenic
GCTCAAAAGGGTCTTCACAA+TGG 0.561078 2_3:-4210179 Msa0272680:CDS
TAGTCCCATTTGAGGTAGCA+GGG 0.566660 2_3:+4210259 None:intergenic
CCACCATTGATTCTTGCTGA+AGG 0.574245 2_3:-4210109 Msa0272680:CDS
ATTGATTCTTGCTGAAGGAG+TGG 0.575091 2_3:-4210104 Msa0272680:CDS
CTGAAGGAGTGGAGGAAGCA+CGG 0.592911 2_3:-4210093 Msa0272680:CDS
TTGAGAGTGGAGGGTTGGTG+TGG 0.593756 2_3:+4210303 None:intergenic
AAGAATTGAGAGTGGAGGGT+TGG 0.597424 2_3:+4210298 None:intergenic
TCAGCAAGAATCAATGGTGG+AGG 0.602784 2_3:+4210112 None:intergenic
TTGGTGGGTAGTCCCATTTG+AGG 0.608890 2_3:+4210251 None:intergenic
GAGGTAGCAGGGGATTTGTG+TGG 0.609669 2_3:+4210270 None:intergenic
AGGCTTGCAAGGAAACCCCT+TGG 0.615306 2_3:-4210034 Msa0272680:CDS
GATTCTTGCTGAAGGAGTGG+AGG 0.617592 2_3:-4210101 Msa0272680:CDS
AGGTTGCTGGAATGGACACT+TGG 0.621740 2_3:+4210132 None:intergenic
CTATGAAGAATTGAGAGTGG+AGG 0.622479 2_3:+4210293 None:intergenic
AGTCCCATTTGAGGTAGCAG+GGG 0.633905 2_3:+4210260 None:intergenic
GGAGTGGAGGAAGCACGGTG+GGG 0.635320 2_3:-4210088 Msa0272680:CDS
TGGCTATGAAGAATTGAGAG+TGG 0.639401 2_3:+4210290 None:intergenic
TATGAAGAATTGAGAGTGGA+GGG 0.652100 2_3:+4210294 None:intergenic
AAGGAGTGGAGGAAGCACGG+TGG 0.655059 2_3:-4210090 Msa0272680:CDS
CACGGTGGGGAGTTGAACAA+AGG 0.655259 2_3:-4210075 Msa0272680:CDS
GGGTTAACATTTGTGCCAAG+GGG 0.655878 2_3:+4210019 None:intergenic
TGAGCATGGAAGATACAACA+TGG 0.667356 2_3:-4210372 None:intergenic
CCTTCAGCAAGAATCAATGG+TGG 0.683493 2_3:+4210109 None:intergenic
GAGCATGGAAGATACAACAT+GGG 0.707943 2_3:-4210371 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATTAGGTAATAAAAT+AGG + chr2_3:4210324-4210343 None:intergenic 10.0%
!! GTAATAAAATAGGTATAAGA+GGG + chr2_3:4210314-4210333 None:intergenic 20.0%
! AATTATCATTCTTGCTCAAA+AGG - chr2_3:4210118-4210137 Msa0272680:CDS 25.0%
! ATTATCATTCTTGCTCAAAA+GGG - chr2_3:4210119-4210138 Msa0272680:CDS 25.0%
! GGTAATAAAATAGGTATAAG+AGG + chr2_3:4210315-4210334 None:intergenic 25.0%
! AGTGACAACAACAAAATATT+AGG + chr2_3:4210336-4210355 None:intergenic 25.0%
!!! TTTTTTGAGAAGAGATGGAT+TGG + chr2_3:4210094-4210113 None:intergenic 30.0%
ATCCATCTCTTCTCAAAAAA+TGG - chr2_3:4210094-4210113 Msa0272680:CDS 30.0%
TCCATCTCTTCTCAAAAAAT+GGG - chr2_3:4210095-4210114 Msa0272680:CDS 30.0%
AACAAAGGAAGAGTTATGTT+AGG - chr2_3:4210250-4210269 Msa0272680:CDS 30.0%
! TGTTGTTGTCACTTATGATA+TGG - chr2_3:4210343-4210362 Msa0272680:CDS 30.0%
GTTTGTAAGGATGTGTGTTA+GGG + chr2_3:4209980-4209999 None:intergenic 35.0%
TATGAAGAATTGAGAGTGGA+GGG + chr2_3:4210019-4210038 None:intergenic 35.0%
!!! TCCCATTTTTTGAGAAGAGA+TGG + chr2_3:4210099-4210118 None:intergenic 35.0%
TGGGTATTCCTAAAACTTCT+TGG - chr2_3:4210151-4210170 Msa0272680:CDS 35.0%
GTATTCCTAAAACTTCTTGG+AGG - chr2_3:4210154-4210173 Msa0272680:CDS 35.0%
GGAAGAGTTATGTTAGGAAA+AGG - chr2_3:4210256-4210275 Msa0272680:CDS 35.0%
GGTTTGTAAGGATGTGTGTT+AGG + chr2_3:4209981-4210000 None:intergenic 40.0%
CTATGAAGAATTGAGAGTGG+AGG + chr2_3:4210020-4210039 None:intergenic 40.0%
TGGCTATGAAGAATTGAGAG+TGG + chr2_3:4210023-4210042 None:intergenic 40.0%
CTCAAAAGGGTCTTCACAAT+GGG - chr2_3:4210132-4210151 Msa0272680:CDS 40.0%
! TTGGACCTCCAAGAAGTTTT+AGG + chr2_3:4210162-4210181 None:intergenic 40.0%
ACTCCTTCAGCAAGAATCAA+TGG + chr2_3:4210207-4210226 None:intergenic 40.0%
! ATTGATTCTTGCTGAAGGAG+TGG - chr2_3:4210206-4210225 Msa0272680:CDS 40.0%
ATGTTAGGAAAAGGCTTGCA+AGG - chr2_3:4210265-4210284 Msa0272680:CDS 40.0%
AGGGTTAACATTTGTGCCAA+GGG + chr2_3:4210295-4210314 None:intergenic 40.0%
TAAGGATGTGTGTTAGGGCT+TGG + chr2_3:4209975-4209994 None:intergenic 45.0%
AAGAATTGAGAGTGGAGGGT+TGG + chr2_3:4210015-4210034 None:intergenic 45.0%
AAATCCCCTGCTACCTCAAA+TGG - chr2_3:4210046-4210065 Msa0272680:CDS 45.0%
AATCCCCTGCTACCTCAAAT+GGG - chr2_3:4210047-4210066 Msa0272680:CDS 45.0%
! TAGTCCCATTTGAGGTAGCA+GGG + chr2_3:4210054-4210073 None:intergenic 45.0%
GCTCAAAAGGGTCTTCACAA+TGG - chr2_3:4210131-4210150 Msa0272680:CDS 45.0%
TCAGCAAGAATCAATGGTGG+AGG + chr2_3:4210201-4210220 None:intergenic 45.0%
CCTTCAGCAAGAATCAATGG+TGG + chr2_3:4210204-4210223 None:intergenic 45.0%
!! CCACCATTGATTCTTGCTGA+AGG - chr2_3:4210201-4210220 Msa0272680:CDS 45.0%
GGGTTAACATTTGTGCCAAG+GGG + chr2_3:4210294-4210313 None:intergenic 45.0%
GAGGGTTAACATTTGTGCCA+AGG + chr2_3:4210296-4210315 None:intergenic 45.0%
GTGTGGTTGTGGGGTTTGTA+AGG + chr2_3:4209993-4210012 None:intergenic 50.0%
! AGTCCCATTTGAGGTAGCAG+GGG + chr2_3:4210053-4210072 None:intergenic 50.0%
! GTAGTCCCATTTGAGGTAGC+AGG + chr2_3:4210055-4210074 None:intergenic 50.0%
! TTGGTGGGTAGTCCCATTTG+AGG + chr2_3:4210062-4210081 None:intergenic 50.0%
! GATTGGAGCAGAGGATTGGT+GGG + chr2_3:4210077-4210096 None:intergenic 50.0%
!! GATGGATTGGAGCAGAGGAT+TGG + chr2_3:4210081-4210100 None:intergenic 50.0%
!! GAAGAGATGGATTGGAGCAG+AGG + chr2_3:4210086-4210105 None:intergenic 50.0%
AGGTTGCTGGAATGGACACT+TGG + chr2_3:4210181-4210200 None:intergenic 50.0%
! AATGGTGGAGGTTGCTGGAA+TGG + chr2_3:4210189-4210208 None:intergenic 50.0%
! GAATCAATGGTGGAGGTTGC+TGG + chr2_3:4210194-4210213 None:intergenic 50.0%
GATTCTTGCTGAAGGAGTGG+AGG - chr2_3:4210209-4210228 Msa0272680:CDS 50.0%
!! TGGAGGGTTGGTGTGGTTGT+GGG + chr2_3:4210003-4210022 None:intergenic 55.0%
!! TTGAGAGTGGAGGGTTGGTG+TGG + chr2_3:4210010-4210029 None:intergenic 55.0%
GAGGTAGCAGGGGATTTGTG+TGG + chr2_3:4210043-4210062 None:intergenic 55.0%
GGATTGGAGCAGAGGATTGG+TGG + chr2_3:4210078-4210097 None:intergenic 55.0%
CTGAAGGAGTGGAGGAAGCA+CGG - chr2_3:4210217-4210236 Msa0272680:CDS 55.0%
CACGGTGGGGAGTTGAACAA+AGG - chr2_3:4210235-4210254 Msa0272680:CDS 55.0%
AGGCTTGCAAGGAAACCCCT+TGG - chr2_3:4210276-4210295 Msa0272680:CDS 55.0%
!! GGAGGGTTGGTGTGGTTGTG+GGG + chr2_3:4210002-4210021 None:intergenic 60.0%
!! GTGGAGGGTTGGTGTGGTTG+TGG + chr2_3:4210004-4210023 None:intergenic 60.0%
! AAGGAGTGGAGGAAGCACGG+TGG - chr2_3:4210220-4210239 Msa0272680:CDS 60.0%
! AGGAGTGGAGGAAGCACGGT+GGG - chr2_3:4210221-4210240 Msa0272680:CDS 60.0%
! GGAGTGGAGGAAGCACGGTG+GGG - chr2_3:4210222-4210241 Msa0272680:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 4209943 4210389 4209943 ID=Msa0272680;Name=Msa0272680
chr2_3 mRNA 4209943 4210389 4209943 ID=Msa0272680-mRNA-1;Parent=Msa0272680;Name=Msa0272680-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|148
chr2_3 exon 4209943 4210389 4209943 ID=Msa0272680-mRNA-1:exon:2105;Parent=Msa0272680-mRNA-1
chr2_3 CDS 4209943 4210389 4209943 ID=Msa0272680-mRNA-1:cds;Parent=Msa0272680-mRNA-1
Gene Sequence

>Msa0272680

ATGGAAGATACAACATGGGAGCAGAAACTCCAAGCCCTAACACACATCCTTACAAACCCCACAACCACACCAACCCTCCACTCTCAATTCTTCATAGCCACACAAATCCCCTGCTACCTCAAATGGGACTACCCACCAATCCTCTGCTCCAATCCATCTCTTCTCAAAAAATGGGAATTATCATTCTTGCTCAAAAGGGTCTTCACAATGGGTATTCCTAAAACTTCTTGGAGGTCCAAGTGTCCATTCCAGCAACCTCCACCATTGATTCTTGCTGAAGGAGTGGAGGAAGCACGGTGGGGAGTTGAACAAAGGAAGAGTTATGTTAGGAAAAGGCTTGCAAGGAAACCCCTTGGCACAAATGTTAACCCTCTTATACCTATTTTATTACCTAATATTTTGTTGTTGTCACTTATGATATGGAATCCATTTAGTTATCTTGATTGA

Protein sequence

>Msa0272680

MEDTTWEQKLQALTHILTNPTTTPTLHSQFFIATQIPCYLKWDYPPILCSNPSLLKKWELSFLLKRVFTMGIPKTSWRSKCPFQQPPPLILAEGVEEARWGVEQRKSYVRKRLARKPLGTNVNPLIPILLPNILLLSLMIWNPFSYLD*