AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192520


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192520 MtrunA17_Chr2g0282711 95.270 148 7 0 1 148 3 150 8.00e-85 245
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192520 AT2G33855.1 55.944 143 61 1 1 143 1 141 7.64e-48 151

Find 58 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGAGGGTTGGTGTGGTTGT+GGG 0.269536 2_1:-8340361 None:intergenic
GGTTTGTAAGGATGTGTGTT+AGG 0.271091 2_1:-8340339 None:intergenic
GTGGAGGGTTGGTGTGGTTG+TGG 0.275633 2_1:-8340362 None:intergenic
GTGTGGTTGTGGGGTTTGTA+AGG 0.288036 2_1:-8340351 None:intergenic
TATCAATCAAGATAACTAAA+TGG 0.290563 2_1:-8340730 None:intergenic
TGGGTATTCCTAAAACTTCT+TGG 0.291474 2_1:+8340512 Msa0192520:CDS
GGTAATAAAATAGGTATAAG+AGG 0.312742 2_1:-8340673 None:intergenic
AGTGACAACAACAAAATATT+AGG 0.321966 2_1:-8340694 None:intergenic
TGTTGTTGTCACTTATGATA+TGG 0.343100 2_1:+8340704 Msa0192520:CDS
AAAATATTAGGTAATAAAAT+AGG 0.344419 2_1:-8340682 None:intergenic
AATTATCATTCTTGCTCAAA+AGG 0.344967 2_1:+8340479 Msa0192520:CDS
AAATCCCCTGCTACCTCAAA+TGG 0.355642 2_1:+8340407 Msa0192520:CDS
GAATCAATGGTGGAGGTTGC+TGG 0.357663 2_1:-8340552 None:intergenic
TAAGGATGTGTGTTAGGGCT+TGG 0.394935 2_1:-8340333 None:intergenic
GTTTGTAAGGATGTGTGTTA+GGG 0.413826 2_1:-8340338 None:intergenic
AACAAAGGAAGAGTTATGTT+AGG 0.432276 2_1:+8340611 Msa0192520:CDS
GGAAGAGTTATGTTAGGAAA+AGG 0.438245 2_1:+8340617 Msa0192520:CDS
GATGGATTGGAGCAGAGGAT+TGG 0.459996 2_1:-8340439 None:intergenic
CTCAAAAGGGTCTTCACAAT+GGG 0.476531 2_1:+8340493 Msa0192520:CDS
GAAGAGATGGATTGGAGCAG+AGG 0.480756 2_1:-8340444 None:intergenic
GGAGGGTTGGTGTGGTTGTG+GGG 0.493337 2_1:-8340360 None:intergenic
AGGGTTAACATTTGTGCCAA+GGG 0.493789 2_1:-8340653 None:intergenic
GGATTGGAGCAGAGGATTGG+TGG 0.495688 2_1:-8340436 None:intergenic
ATTATCATTCTTGCTCAAAA+GGG 0.496190 2_1:+8340480 Msa0192520:CDS
GTAGTCCCATTTGAGGTAGC+AGG 0.497802 2_1:-8340413 None:intergenic
ACTCCTTCAGCAAGAATCAA+TGG 0.503351 2_1:-8340565 None:intergenic
AGGAGTGGAGGAAGCACGGT+GGG 0.505781 2_1:+8340582 Msa0192520:CDS
AATGGTGGAGGTTGCTGGAA+TGG 0.508912 2_1:-8340547 None:intergenic
AATCCCCTGCTACCTCAAAT+GGG 0.518789 2_1:+8340408 Msa0192520:CDS
ATGTTAGGAAAAGGCTTGCA+AGG 0.547320 2_1:+8340626 Msa0192520:CDS
GTATTCCTAAAACTTCTTGG+AGG 0.547416 2_1:+8340515 Msa0192520:CDS
GAGGGTTAACATTTGTGCCA+AGG 0.547578 2_1:-8340654 None:intergenic
GATTGGAGCAGAGGATTGGT+GGG 0.557001 2_1:-8340435 None:intergenic
GTAATAAAATAGGTATAAGA+GGG 0.558203 2_1:-8340672 None:intergenic
GCTCAAAAGGGTCTTCACAA+TGG 0.561078 2_1:+8340492 Msa0192520:CDS
TAGTCCCATTTGAGGTAGCA+GGG 0.566660 2_1:-8340412 None:intergenic
CCACCATTGATTCTTGCTGA+AGG 0.574245 2_1:+8340562 Msa0192520:CDS
ATTGATTCTTGCTGAAGGAG+TGG 0.575091 2_1:+8340567 Msa0192520:CDS
CTGAAGGAGTGGAGGAAGCA+CGG 0.592911 2_1:+8340578 Msa0192520:CDS
TTGAGAGTGGAGGGTTGGTG+TGG 0.593756 2_1:-8340368 None:intergenic
AAGAATTGAGAGTGGAGGGT+TGG 0.597424 2_1:-8340373 None:intergenic
TCAGCAAGAATCAATGGTGG+AGG 0.602784 2_1:-8340559 None:intergenic
TTGGTGGGTAGTCCCATTTG+AGG 0.608890 2_1:-8340420 None:intergenic
GAGGTAGCAGGGGATTTGTG+TGG 0.609669 2_1:-8340401 None:intergenic
AGGCTTGCAAGGAAACCCCT+TGG 0.615306 2_1:+8340637 Msa0192520:CDS
GATTCTTGCTGAAGGAGTGG+AGG 0.617592 2_1:+8340570 Msa0192520:CDS
AGGTTGCTGGAATGGACACT+TGG 0.621740 2_1:-8340539 None:intergenic
CTATGAAGAATTGAGAGTGG+AGG 0.622479 2_1:-8340378 None:intergenic
AGTCCCATTTGAGGTAGCAG+GGG 0.633905 2_1:-8340411 None:intergenic
GGAGTGGAGGAAGCACGGTG+GGG 0.635320 2_1:+8340583 Msa0192520:CDS
TGGCTATGAAGAATTGAGAG+TGG 0.639401 2_1:-8340381 None:intergenic
TATGAAGAATTGAGAGTGGA+GGG 0.652100 2_1:-8340377 None:intergenic
AAGGAGTGGAGGAAGCACGG+TGG 0.655059 2_1:+8340581 Msa0192520:CDS
CACGGTGGGGAGTTGAACAA+AGG 0.655259 2_1:+8340596 Msa0192520:CDS
GGGTTAACATTTGTGCCAAG+GGG 0.655878 2_1:-8340652 None:intergenic
TGAGCATGGAAGATACAACA+TGG 0.667356 2_1:+8340299 None:intergenic
CCTTCAGCAAGAATCAATGG+TGG 0.683493 2_1:-8340562 None:intergenic
GAGCATGGAAGATACAACAT+GGG 0.707943 2_1:+8340300 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATTAGGTAATAAAAT+AGG - chr2_1:8340685-8340704 None:intergenic 10.0%
!! GTAATAAAATAGGTATAAGA+GGG - chr2_1:8340675-8340694 None:intergenic 20.0%
! AATTATCATTCTTGCTCAAA+AGG + chr2_1:8340479-8340498 Msa0192520:CDS 25.0%
! ATTATCATTCTTGCTCAAAA+GGG + chr2_1:8340480-8340499 Msa0192520:CDS 25.0%
! GGTAATAAAATAGGTATAAG+AGG - chr2_1:8340676-8340695 None:intergenic 25.0%
! AGTGACAACAACAAAATATT+AGG - chr2_1:8340697-8340716 None:intergenic 25.0%
!!! TTTTTTGAGAAGAGATGGAT+TGG - chr2_1:8340455-8340474 None:intergenic 30.0%
ATCCATCTCTTCTCAAAAAA+TGG + chr2_1:8340455-8340474 Msa0192520:CDS 30.0%
TCCATCTCTTCTCAAAAAAT+GGG + chr2_1:8340456-8340475 Msa0192520:CDS 30.0%
AACAAAGGAAGAGTTATGTT+AGG + chr2_1:8340611-8340630 Msa0192520:CDS 30.0%
! TGTTGTTGTCACTTATGATA+TGG + chr2_1:8340704-8340723 Msa0192520:CDS 30.0%
GTTTGTAAGGATGTGTGTTA+GGG - chr2_1:8340341-8340360 None:intergenic 35.0%
TATGAAGAATTGAGAGTGGA+GGG - chr2_1:8340380-8340399 None:intergenic 35.0%
!!! TCCCATTTTTTGAGAAGAGA+TGG - chr2_1:8340460-8340479 None:intergenic 35.0%
TGGGTATTCCTAAAACTTCT+TGG + chr2_1:8340512-8340531 Msa0192520:CDS 35.0%
GTATTCCTAAAACTTCTTGG+AGG + chr2_1:8340515-8340534 Msa0192520:CDS 35.0%
GGAAGAGTTATGTTAGGAAA+AGG + chr2_1:8340617-8340636 Msa0192520:CDS 35.0%
GGTTTGTAAGGATGTGTGTT+AGG - chr2_1:8340342-8340361 None:intergenic 40.0%
CTATGAAGAATTGAGAGTGG+AGG - chr2_1:8340381-8340400 None:intergenic 40.0%
TGGCTATGAAGAATTGAGAG+TGG - chr2_1:8340384-8340403 None:intergenic 40.0%
CTCAAAAGGGTCTTCACAAT+GGG + chr2_1:8340493-8340512 Msa0192520:CDS 40.0%
! TTGGACCTCCAAGAAGTTTT+AGG - chr2_1:8340523-8340542 None:intergenic 40.0%
ACTCCTTCAGCAAGAATCAA+TGG - chr2_1:8340568-8340587 None:intergenic 40.0%
! ATTGATTCTTGCTGAAGGAG+TGG + chr2_1:8340567-8340586 Msa0192520:CDS 40.0%
ATGTTAGGAAAAGGCTTGCA+AGG + chr2_1:8340626-8340645 Msa0192520:CDS 40.0%
AGGGTTAACATTTGTGCCAA+GGG - chr2_1:8340656-8340675 None:intergenic 40.0%
TAAGGATGTGTGTTAGGGCT+TGG - chr2_1:8340336-8340355 None:intergenic 45.0%
AAGAATTGAGAGTGGAGGGT+TGG - chr2_1:8340376-8340395 None:intergenic 45.0%
AAATCCCCTGCTACCTCAAA+TGG + chr2_1:8340407-8340426 Msa0192520:CDS 45.0%
AATCCCCTGCTACCTCAAAT+GGG + chr2_1:8340408-8340427 Msa0192520:CDS 45.0%
! TAGTCCCATTTGAGGTAGCA+GGG - chr2_1:8340415-8340434 None:intergenic 45.0%
GCTCAAAAGGGTCTTCACAA+TGG + chr2_1:8340492-8340511 Msa0192520:CDS 45.0%
TCAGCAAGAATCAATGGTGG+AGG - chr2_1:8340562-8340581 None:intergenic 45.0%
CCTTCAGCAAGAATCAATGG+TGG - chr2_1:8340565-8340584 None:intergenic 45.0%
!! CCACCATTGATTCTTGCTGA+AGG + chr2_1:8340562-8340581 Msa0192520:CDS 45.0%
GGGTTAACATTTGTGCCAAG+GGG - chr2_1:8340655-8340674 None:intergenic 45.0%
GAGGGTTAACATTTGTGCCA+AGG - chr2_1:8340657-8340676 None:intergenic 45.0%
GTGTGGTTGTGGGGTTTGTA+AGG - chr2_1:8340354-8340373 None:intergenic 50.0%
! AGTCCCATTTGAGGTAGCAG+GGG - chr2_1:8340414-8340433 None:intergenic 50.0%
! GTAGTCCCATTTGAGGTAGC+AGG - chr2_1:8340416-8340435 None:intergenic 50.0%
! TTGGTGGGTAGTCCCATTTG+AGG - chr2_1:8340423-8340442 None:intergenic 50.0%
! GATTGGAGCAGAGGATTGGT+GGG - chr2_1:8340438-8340457 None:intergenic 50.0%
!! GATGGATTGGAGCAGAGGAT+TGG - chr2_1:8340442-8340461 None:intergenic 50.0%
!! GAAGAGATGGATTGGAGCAG+AGG - chr2_1:8340447-8340466 None:intergenic 50.0%
AGGTTGCTGGAATGGACACT+TGG - chr2_1:8340542-8340561 None:intergenic 50.0%
! AATGGTGGAGGTTGCTGGAA+TGG - chr2_1:8340550-8340569 None:intergenic 50.0%
! GAATCAATGGTGGAGGTTGC+TGG - chr2_1:8340555-8340574 None:intergenic 50.0%
GATTCTTGCTGAAGGAGTGG+AGG + chr2_1:8340570-8340589 Msa0192520:CDS 50.0%
!! TGGAGGGTTGGTGTGGTTGT+GGG - chr2_1:8340364-8340383 None:intergenic 55.0%
!! TTGAGAGTGGAGGGTTGGTG+TGG - chr2_1:8340371-8340390 None:intergenic 55.0%
GAGGTAGCAGGGGATTTGTG+TGG - chr2_1:8340404-8340423 None:intergenic 55.0%
GGATTGGAGCAGAGGATTGG+TGG - chr2_1:8340439-8340458 None:intergenic 55.0%
CTGAAGGAGTGGAGGAAGCA+CGG + chr2_1:8340578-8340597 Msa0192520:CDS 55.0%
CACGGTGGGGAGTTGAACAA+AGG + chr2_1:8340596-8340615 Msa0192520:CDS 55.0%
AGGCTTGCAAGGAAACCCCT+TGG + chr2_1:8340637-8340656 Msa0192520:CDS 55.0%
!! GGAGGGTTGGTGTGGTTGTG+GGG - chr2_1:8340363-8340382 None:intergenic 60.0%
!! GTGGAGGGTTGGTGTGGTTG+TGG - chr2_1:8340365-8340384 None:intergenic 60.0%
! AAGGAGTGGAGGAAGCACGG+TGG + chr2_1:8340581-8340600 Msa0192520:CDS 60.0%
! AGGAGTGGAGGAAGCACGGT+GGG + chr2_1:8340582-8340601 Msa0192520:CDS 60.0%
! GGAGTGGAGGAAGCACGGTG+GGG + chr2_1:8340583-8340602 Msa0192520:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8340304 8340750 8340304 ID=Msa0192520;Name=Msa0192520
chr2_1 mRNA 8340304 8340750 8340304 ID=Msa0192520-mRNA-1;Parent=Msa0192520;Name=Msa0192520-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|148
chr2_1 exon 8340304 8340750 8340304 ID=Msa0192520-mRNA-1:exon:3819;Parent=Msa0192520-mRNA-1
chr2_1 CDS 8340304 8340750 8340304 ID=Msa0192520-mRNA-1:cds;Parent=Msa0192520-mRNA-1
Gene Sequence

>Msa0192520

ATGGAAGATACAACATGGGAGCAGAAACTCCAAGCCCTAACACACATCCTTACAAACCCCACAACCACACCAACCCTCCACTCTCAATTCTTCATAGCCACACAAATCCCCTGCTACCTCAAATGGGACTACCCACCAATCCTCTGCTCCAATCCATCTCTTCTCAAAAAATGGGAATTATCATTCTTGCTCAAAAGGGTCTTCACAATGGGTATTCCTAAAACTTCTTGGAGGTCCAAGTGTCCATTCCAGCAACCTCCACCATTGATTCTTGCTGAAGGAGTGGAGGAAGCACGGTGGGGAGTTGAACAAAGGAAGAGTTATGTTAGGAAAAGGCTTGCAAGGAAACCCCTTGGCACAAATGTTAACCCTCTTATACCTATTTTATTACCTAATATTTTGTTGTTGTCACTTATGATATGGAATCCATTTAGTTATCTTGATTGA

Protein sequence

>Msa0192520

MEDTTWEQKLQALTHILTNPTTTPTLHSQFFIATQIPCYLKWDYPPILCSNPSLLKKWELSFLLKRVFTMGIPKTSWRSKCPFQQPPPLILAEGVEEARWGVEQRKSYVRKRLARKPLGTNVNPLIPILLPNILLLSLMIWNPFSYLD*