AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0556960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0556960 MtrunA17_Chr4g0045941 96.875 352 11 0 1 352 1 352 0.0 713
Msa0556960 MtrunA17_Chr4g0045951 83.516 364 48 1 1 352 1 364 0.0 629
Msa0556960 MtrunA17_Chr2g0283581 71.191 361 94 3 1 352 1 360 0.0 528
Msa0556960 MtrunA17_Chr2g0325481 60.991 323 123 2 30 351 47 367 1.17e-149 426
Msa0556960 MtrunA17_Chr4g0041011 59.190 321 130 1 30 350 44 363 3.70e-141 404
Msa0556960 MtrunA17_Chr3g0097891 53.602 347 158 3 8 351 5 351 9.30e-137 393
Msa0556960 MtrunA17_Chr2g0314801 52.616 344 157 4 9 346 6 349 1.23e-130 377
Msa0556960 MtrunA17_Chr3g0085081 51.327 339 160 3 16 350 11 348 2.74e-127 369
Msa0556960 MtrunA17_Chr3g0085071 52.174 322 152 2 30 350 43 363 4.26e-127 369
Msa0556960 MtrunA17_Chr3g0091031 49.704 338 165 3 14 350 15 348 1.13e-125 364
Msa0556960 MtrunA17_Chr5g0413851 50.284 352 166 4 7 351 11 360 9.09e-125 363
Msa0556960 MtrunA17_Chr3g0105301 48.986 345 160 4 23 351 33 377 4.31e-118 346
Msa0556960 MtrunA17_Chr5g0430371 49.580 357 152 6 23 351 44 400 8.18e-118 346
Msa0556960 MtrunA17_Chr2g0314791 45.278 360 174 9 1 351 1 346 1.22e-106 316
Msa0556960 MtrunA17_Chr1g0175671 38.776 343 186 6 33 352 170 511 1.07e-89 278
Msa0556960 MtrunA17_Chr1g0171171 40.361 332 181 5 30 350 50 375 9.63e-89 271
Msa0556960 MtrunA17_Chr7g0258851 39.942 343 182 6 33 352 248 589 5.17e-88 276
Msa0556960 MtrunA17_Chr4g0068111 39.489 352 183 9 27 352 108 455 5.65e-85 265
Msa0556960 MtrunA17_Chr1g0210771 36.826 334 185 7 33 350 59 382 1.04e-81 253
Msa0556960 MtrunA17_Chr4g0071391 36.657 341 194 5 33 352 109 448 2.86e-81 254
Msa0556960 MtrunA17_Chr1g0213281 37.101 345 192 7 31 351 173 516 7.36e-80 253
Msa0556960 MtrunA17_Chr5g0413271 36.499 337 192 5 33 351 145 477 1.04e-77 246
Msa0556960 MtrunA17_Chr4g0006991 36.550 342 193 7 33 350 86 427 2.45e-76 241
Msa0556960 MtrunA17_Chr3g0113671 36.782 348 190 8 32 351 105 450 3.77e-75 239
Msa0556960 MtrunA17_Chr8g0368461 35.389 373 200 10 19 351 158 529 1.36e-74 239
Msa0556960 MtrunA17_Chr7g0269991 37.994 329 187 7 33 346 137 463 1.65e-73 235
Msa0556960 MtrunA17_Chr5g0437291 37.110 353 185 11 32 351 164 512 4.12e-72 233
Msa0556960 MtrunA17_Chr4g0005491 34.943 352 201 8 27 350 56 407 6.62e-71 226
Msa0556960 MtrunA17_Chr3g0114101 34.985 343 199 8 33 351 89 431 6.75e-71 227
Msa0556960 MtrunA17_Chr8g0360521 35.652 345 195 8 30 350 68 409 5.15e-70 224
Msa0556960 MtrunA17_Chr7g0243301 35.817 349 196 9 30 350 61 409 2.56e-68 220
Msa0556960 MtrunA17_Chr1g0209921 43.701 254 133 8 9 255 17 267 5.66e-68 214
Msa0556960 MtrunA17_Chr3g0126021 37.714 350 189 9 25 351 117 460 5.02e-67 218
Msa0556960 MtrunA17_Chr7g0242701 35.362 345 199 7 30 350 83 427 7.53e-67 216
Msa0556960 MtrunA17_Chr7g0259711 35.613 351 198 9 27 351 85 433 1.85e-66 216
Msa0556960 MtrunA17_Chr2g0287901 33.333 348 207 7 28 351 92 438 2.03e-66 216
Msa0556960 MtrunA17_Chr5g0437601 33.618 351 209 8 25 351 75 425 3.51e-66 215
Msa0556960 MtrunA17_Chr4g0005481 34.493 345 199 9 26 346 101 442 1.36e-64 211
Msa0556960 MtrunA17_Chr8g0384151 34.030 335 198 7 33 351 90 417 1.10e-62 206
Msa0556960 MtrunA17_Chr1g0178081 31.117 376 177 11 3 351 22 342 5.65e-48 165
Msa0556960 MtrunA17_Chr5g0414061 30.114 352 205 11 26 343 87 431 2.64e-39 144
Msa0556960 MtrunA17_Chr6g0456681 30.894 369 208 12 15 346 46 404 2.08e-36 136
Msa0556960 MtrunA17_Chr6g0456691 29.513 349 214 8 32 352 77 421 2.05e-35 134
Msa0556960 MtrunA17_Chr5g0414091 30.321 343 206 10 32 343 84 424 2.82e-35 133
Msa0556960 MtrunA17_Chr6g0476041 28.226 372 207 12 20 346 154 510 7.23e-35 133
Msa0556960 MtrunA17_Chr4g0049421 28.775 351 213 13 33 351 48 393 1.02e-34 131
Msa0556960 MtrunA17_Chr3g0085101 56.604 106 45 1 217 321 1 106 2.13e-34 122
Msa0556960 MtrunA17_Chr1g0188191 28.371 356 207 12 30 346 231 577 5.15e-33 129
Msa0556960 MtrunA17_Chr8g0384421 29.513 349 214 11 29 350 129 472 5.47e-33 127
Msa0556960 MtrunA17_Chr8g0347321 29.396 364 200 15 30 351 92 440 9.96e-33 126
Msa0556960 MtrunA17_Chr4g0070341 30.471 361 211 16 24 350 117 471 1.41e-31 124
Msa0556960 MtrunA17_Chr5g0414051 28.412 359 195 16 33 346 75 416 4.16e-31 122
Msa0556960 MtrunA17_Chr5g0414041 26.801 347 217 9 33 346 87 429 1.69e-30 120
Msa0556960 MtrunA17_Chr6g0451321 28.611 360 213 14 24 350 109 457 1.20e-29 118
Msa0556960 MtrunA17_Chr2g0283851 27.869 366 211 16 22 346 43 396 1.47e-29 117
Msa0556960 MtrunA17_Chr3g0140371 27.586 348 213 13 31 346 16 356 1.51e-29 116
Msa0556960 MtrunA17_Chr7g0226991 27.378 347 214 11 33 346 57 398 2.10e-29 117
Msa0556960 MtrunA17_Chr8g0387921 26.346 353 226 9 30 351 71 420 1.85e-28 114
Msa0556960 MtrunA17_Chr8g0391481 27.049 366 220 12 21 346 10 368 3.25e-28 113
Msa0556960 MtrunA17_Chr6g0455901 27.557 352 215 12 29 346 67 412 2.88e-25 105
Msa0556960 MtrunA17_Chr1g0209931 51.685 89 39 2 264 351 8 93 4.17e-22 89.7
Msa0556960 MtrunA17_Chr7g0221271 35.433 127 82 0 33 159 79 205 1.30e-21 91.3
Msa0556960 MtrunA17_Chr5g0429211 51.807 83 39 1 79 161 187 268 2.15e-20 89.7
Msa0556960 MtrunA17_Chr7g0221291 40.217 92 55 0 30 121 76 167 1.21e-19 85.1
Msa0556960 MtrunA17_Chr2g0287931 29.600 125 74 3 93 203 2 126 2.18e-13 66.2
Msa0556960 MtrunA17_Chr2g0287911 46.774 62 26 1 27 88 55 109 1.18e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0556960 AT1G29050.1 64.865 333 115 2 21 352 49 380 6.04e-172 483
Msa0556960 AT2G34070.1 64.832 327 112 3 28 352 60 385 1.40e-165 468
Msa0556960 AT2G31110.2 58.491 318 132 0 33 350 45 362 2.12e-148 423
Msa0556960 AT2G42570.1 58.589 326 135 0 26 351 40 365 2.14e-146 418
Msa0556960 AT3G14850.2 53.870 323 144 3 28 346 29 350 6.43e-127 368
Msa0556960 AT2G30900.1 49.435 354 167 5 10 351 13 366 7.01e-122 356
Msa0556960 AT2G34070.2 66.270 252 82 3 103 352 1 251 7.43e-122 351
Msa0556960 AT1G78710.1 48.012 327 165 4 28 351 33 357 2.65e-119 349
Msa0556960 AT5G58600.1 50.592 338 150 5 31 351 64 401 9.77e-116 342
Msa0556960 AT2G30010.1 47.059 357 161 6 23 351 41 397 8.74e-109 323
Msa0556960 AT2G30010.2 47.059 357 161 6 23 351 84 440 1.73e-108 324
Msa0556960 AT2G31110.3 58.986 217 89 0 33 249 45 261 1.32e-100 298
Msa0556960 AT3G14850.1 54.435 248 108 3 103 346 1 247 6.46e-94 280
Msa0556960 AT2G31110.1 59.420 207 84 0 144 350 8 214 3.39e-93 277
Msa0556960 AT5G06700.1 40.407 344 181 6 32 352 254 596 3.62e-89 280
Msa0556960 AT1G78710.2 40.673 327 136 5 28 351 26 297 1.57e-86 263
Msa0556960 AT3G12060.1 40.000 345 183 6 31 352 194 537 1.59e-86 271
Msa0556960 AT3G54260.1 39.104 335 178 7 33 351 51 375 2.04e-85 263
Msa0556960 AT2G30900.2 49.219 256 121 3 10 256 13 268 7.23e-85 258
Msa0556960 AT5G58600.2 52.632 228 106 2 31 256 64 291 2.72e-83 254
Msa0556960 AT5G20590.1 37.288 354 199 6 21 352 129 481 4.11e-83 261
Msa0556960 AT5G01360.2 36.812 345 193 7 31 350 34 378 1.59e-79 248
Msa0556960 AT5G49340.1 35.040 371 215 7 7 352 74 443 2.81e-79 250
Msa0556960 AT5G01360.1 36.812 345 193 7 31 350 90 434 7.15e-79 248
Msa0556960 AT3G62390.1 38.012 342 192 8 28 351 133 472 6.76e-76 242
Msa0556960 AT1G60790.1 36.127 346 194 7 32 351 189 533 8.39e-75 241
Msa0556960 AT1G73140.1 36.207 348 195 8 28 351 62 406 1.87e-72 231
Msa0556960 AT5G19160.1 36.134 357 190 11 28 351 94 445 5.08e-71 229
Msa0556960 AT2G40150.1 34.930 355 195 9 31 351 69 421 9.62e-71 227
Msa0556960 AT3G55990.1 36.103 349 193 9 31 351 138 484 2.72e-70 228
Msa0556960 AT3G11030.1 35.411 353 201 9 25 351 98 449 7.69e-70 225
Msa0556960 AT5G06230.2 34.097 349 202 7 30 351 18 365 4.05e-69 221
Msa0556960 AT5G06230.1 34.097 349 202 7 30 351 59 406 7.84e-69 221
Msa0556960 AT1G48880.1 35.522 335 183 7 32 346 114 435 9.65e-69 222
Msa0556960 AT3G06080.2 34.286 350 203 7 28 351 98 446 3.43e-68 221
Msa0556960 AT5G01620.2 34.097 349 203 9 23 346 99 445 2.62e-67 219
Msa0556960 AT5G01620.1 34.097 349 203 9 23 346 99 445 2.62e-67 219
Msa0556960 AT2G40160.1 34.670 349 197 9 33 351 77 424 3.39e-67 218
Msa0556960 AT5G01620.3 34.097 349 203 9 23 346 107 453 3.91e-67 218
Msa0556960 AT2G40160.2 34.384 349 198 9 33 351 77 424 6.65e-67 217
Msa0556960 AT2G40320.1 35.694 353 199 10 25 351 73 423 7.25e-67 217
Msa0556960 AT2G38320.1 33.718 347 202 8 33 351 59 405 6.27e-66 214
Msa0556960 AT3G11570.1 34.568 324 190 6 49 351 98 420 5.82e-65 212
Msa0556960 AT2G38320.2 31.975 319 190 7 60 351 4 322 2.83e-55 184
Msa0556960 AT5G20590.2 35.887 248 145 4 21 254 129 376 3.38e-53 180
Msa0556960 AT5G19160.2 35.685 241 141 5 28 254 94 334 1.61e-51 175
Msa0556960 AT3G62390.2 35.983 239 141 5 28 254 133 371 1.14e-49 172
Msa0556960 AT2G40160.3 33.761 234 143 4 33 254 77 310 5.53e-46 159
Msa0556960 AT3G06080.1 33.610 241 146 4 28 254 98 338 3.09e-45 158
Msa0556960 AT5G06230.3 31.984 247 148 4 30 256 59 305 7.37e-43 151
Msa0556960 AT4G25360.1 30.447 358 205 12 27 346 169 520 2.34e-38 144
Msa0556960 AT4G25360.2 30.447 358 205 12 27 346 169 520 2.34e-38 144
Msa0556960 AT5G64470.2 31.389 360 194 16 33 352 55 401 6.31e-38 140
Msa0556960 AT5G64470.3 30.312 353 201 14 33 352 55 395 2.63e-37 138
Msa0556960 AT5G51640.1 30.108 372 213 12 15 346 120 484 4.18e-37 140
Msa0556960 AT1G70230.1 28.783 337 209 12 33 346 79 407 3.53e-36 135
Msa0556960 AT1G01430.1 27.824 363 221 11 25 351 91 448 2.14e-35 134
Msa0556960 AT5G64020.1 29.050 358 208 12 33 350 57 408 6.87e-35 132
Msa0556960 AT2G37720.1 28.451 355 202 13 33 350 143 482 2.72e-32 126
Msa0556960 AT5G15900.1 27.049 366 232 9 15 350 49 409 4.03e-31 122
Msa0556960 AT5G15900.2 27.049 366 232 9 15 350 68 428 5.29e-31 122
Msa0556960 AT5G20680.6 28.116 345 216 13 33 350 118 457 1.52e-30 121
Msa0556960 AT3G28150.1 27.595 395 214 13 9 350 35 410 2.56e-30 120
Msa0556960 AT5G20680.4 28.116 345 216 13 33 350 212 551 2.87e-30 121
Msa0556960 AT5G20680.1 28.116 345 216 13 33 350 212 551 2.87e-30 121
Msa0556960 AT5G20680.3 28.116 345 216 13 33 350 212 551 2.87e-30 121
Msa0556960 AT5G20680.5 28.116 345 216 13 33 350 212 551 2.87e-30 121
Msa0556960 AT5G20680.2 28.116 345 216 13 33 350 194 533 2.88e-30 121
Msa0556960 AT5G15890.1 27.684 354 223 10 20 346 171 518 9.01e-30 119
Msa0556960 AT3G02440.1 26.744 344 216 10 33 346 127 464 2.34e-29 118
Msa0556960 AT3G02440.2 26.744 344 216 10 33 346 135 472 2.67e-29 118
Msa0556960 AT4G01080.1 28.242 347 211 13 30 346 88 426 3.32e-28 114
Msa0556960 AT2G14530.1 27.809 356 214 12 30 350 59 406 3.50e-28 114
Msa0556960 AT4G11090.1 27.298 359 210 13 33 351 79 426 7.03e-27 110
Msa0556960 AT4G23790.1 26.136 352 225 10 33 351 79 428 2.27e-24 103
Msa0556960 AT5G64470.4 28.516 256 147 9 33 262 55 300 2.44e-21 93.6
Msa0556960 AT5G64470.1 28.516 256 147 9 33 262 55 300 3.20e-21 93.2

Find 98 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATTGTAATTTGTTCTT+TGG 0.220399 4_1:+55206728 Msa0556960:CDS
TGAATTATGTCCACCAAATA+TGG 0.229373 4_1:-55209594 None:intergenic
AAAGCCATATGGTCCTATTT+TGG 0.230247 4_1:-55206623 None:intergenic
ACTATACCGTACACCATATT+TGG 0.252473 4_1:+55209581 Msa0556960:CDS
AATTTAATTACTATATATAT+TGG 0.269310 4_1:+55210646 Msa0556960:three_prime_UTR
TATTTAAGATACTGATTATC+AGG 0.276183 4_1:-55206842 None:intergenic
CTTAACCACTTGGGCTGGTT+GGG 0.289743 4_1:+55210050 Msa0556960:CDS
ACATGATTAGAGCTGCATTT+AGG 0.303593 4_1:-55210545 None:intergenic
AGGAGTATGTTCCAAGTATC+AGG 0.327240 4_1:-55210525 None:intergenic
TTGCTGGGTGCATGGATATA+AGG 0.329977 4_1:-55206691 None:intergenic
TTGTTCACTACATCATTTGC+AGG 0.336870 4_1:-55210363 None:intergenic
CAATGTCTACTGTCTTCTTC+CGG 0.356385 4_1:+55207299 Msa0556960:CDS
AAAACTAAACTCTTCTTTCA+AGG 0.357639 4_1:+55210096 Msa0556960:CDS
GGTGATATTGCGCAGGACTA+TGG 0.357643 4_1:+55209546 Msa0556960:intron
GCATGGATATAAGGAGATTT+AGG 0.362396 4_1:-55206682 None:intergenic
CGGGGAAGTTGAACCATTAC+CGG 0.374830 4_1:+55210314 Msa0556960:CDS
ATCAGTATCTTAAATACTCT+TGG 0.387106 4_1:+55206849 Msa0556960:CDS
GGATATGTGGTTCCCGGTAA+TGG 0.391578 4_1:-55210327 None:intergenic
GCTTAACCACTTGGGCTGGT+TGG 0.399226 4_1:+55210049 Msa0556960:CDS
CTTGGTAAAGCACATGAATC+AGG 0.414998 4_1:-55206875 None:intergenic
TGTGAAATTAGAGTATATAT+TGG 0.416927 4_1:+55210565 Msa0556960:exon
AATGTCTACTGTCTTCTTCC+GGG 0.421225 4_1:+55207300 Msa0556960:CDS
AGTGAGGGACATGGACCGTT+TGG 0.425551 4_1:+55210011 Msa0556960:CDS
TCCCCGCTACATGTCTTCTT+CGG 0.430209 4_1:-55210297 None:intergenic
TTGAATCCAGAGGGCAGGAT+TGG 0.450495 4_1:+55210268 Msa0556960:intron
TTACTTACTTGTTATGAGTA+GGG 0.455719 4_1:-55210125 None:intergenic
GTAATTTGTTCTTTGGAAGC+TGG 0.456024 4_1:+55206735 Msa0556960:CDS
TTTCTGTGGTGTAGGTTCAA+TGG 0.457357 4_1:+55207136 Msa0556960:intron
TTTATAAGGGCTTAACCACT+TGG 0.462483 4_1:+55210040 Msa0556960:CDS
ACATCATTTGCAGGAGGTAG+AGG 0.472593 4_1:-55210354 None:intergenic
TGTAGTCATTGGTGCCTACC+AGG 0.474740 4_1:+55210498 Msa0556960:CDS
TTGTGCTCTTGCTGGGTGCA+TGG 0.478358 4_1:-55206699 None:intergenic
TACCAGGACTACCTGATACT+TGG 0.478567 4_1:+55210514 Msa0556960:CDS
GAAAAGCTTGTAGTGACATT+CGG 0.481465 4_1:-55207265 None:intergenic
TAATTTGTTCTTTGGAAGCT+GGG 0.484946 4_1:+55206736 Msa0556960:CDS
GGATAAGAAGATAATGTTTG+TGG 0.487315 4_1:+55207183 Msa0556960:CDS
TGGTGTACGGTATAGTTGTA+TGG 0.491607 4_1:-55209574 None:intergenic
GGATCCTTAGTATAGATAGA+AGG 0.493596 4_1:-55210462 None:intergenic
GTTAAAGAACATGAAGAAGC+AGG 0.504244 4_1:+55210389 Msa0556960:CDS
ATCTATACTAAGGATCCAAC+TGG 0.504484 4_1:+55210468 Msa0556960:CDS
GATAAGAAGATAATGTTTGT+GGG 0.505312 4_1:+55207184 Msa0556960:CDS
ACCACCAAAATAGGACCATA+TGG 0.505449 4_1:+55206619 Msa0556960:exon
CTGGCATTGATTGTAGTCAT+TGG 0.505796 4_1:+55210487 Msa0556960:CDS
ATTACCGGGAACCACATATC+CGG 0.506278 4_1:+55210329 Msa0556960:CDS
TGTGATTTGAAGTCCACATA+TGG 0.507294 4_1:+55206514 Msa0556960:five_prime_UTR
GTCTTGTATGATTCATGCGT+CGG 0.512224 4_1:+55207240 Msa0556960:CDS
AAGGGCTTAACCACTTGGGC+TGG 0.512847 4_1:+55210045 Msa0556960:CDS
ATCAAATTGAGCTTCAACGA+AGG 0.515709 4_1:-55206801 None:intergenic
GTTACTTACTTGTTATGAGT+AGG 0.517658 4_1:-55210126 None:intergenic
TCTCCTTGAATCCAGAGGGC+AGG 0.517922 4_1:+55210263 Msa0556960:intron
ACATCCTTCTATCTATACTA+AGG 0.519367 4_1:+55210458 Msa0556960:CDS
TATGGACATTGAAAGCCATA+TGG 0.524499 4_1:-55206634 None:intergenic
TCTTCAACTCCTGGCACTGG+TGG 0.524966 4_1:+55209691 Msa0556960:CDS
AGTGTGAATCCACCAGTGCC+AGG 0.528738 4_1:-55209700 None:intergenic
AGGTAGAGGGGCCGGATATG+TGG 0.530211 4_1:-55210340 None:intergenic
TACTTACTTGTTATGAGTAG+GGG 0.532035 4_1:-55210124 None:intergenic
TTCCAAGTATCAGGTAGTCC+TGG 0.535659 4_1:-55210516 None:intergenic
TGATCTTCAACTCCTGGCAC+TGG 0.536421 4_1:+55209688 Msa0556960:CDS
CTATAAAAGCTGGTAAAGCA+TGG 0.539195 4_1:+55209649 Msa0556960:CDS
CATCATTTGCAGGAGGTAGA+GGG 0.540823 4_1:-55210353 None:intergenic
AACCGAAGAAGACATGTAGC+GGG 0.541404 4_1:+55210295 Msa0556960:CDS
ACCCTGGACTCTATAAAAGC+TGG 0.546560 4_1:+55209639 Msa0556960:CDS
GACATAATTCAAGAAGATGT+TGG 0.547898 4_1:+55209606 Msa0556960:CDS
TTTGCAGGAGGTAGAGGGGC+CGG 0.548515 4_1:-55210348 None:intergenic
GGGGCCGGATATGTGGTTCC+CGG 0.554885 4_1:-55210333 None:intergenic
TGACTACAATCAATGCCAGT+TGG 0.558982 4_1:-55210483 None:intergenic
ACATGTTGATCTTCAACTCC+TGG 0.559302 4_1:+55209682 Msa0556960:CDS
ATACCGTACACCATATTTGG+TGG 0.560969 4_1:+55209584 Msa0556960:CDS
ATTCATTGAGTCTAAACATG+TGG 0.578501 4_1:+55207209 Msa0556960:CDS
TATAAAAGCTGGTAAAGCAT+GGG 0.581903 4_1:+55209650 Msa0556960:CDS
CCTTATAAAATGCATCCAAA+CGG 0.585186 4_1:-55210026 None:intergenic
TTATAAGGGCTTAACCACTT+GGG 0.588920 4_1:+55210041 Msa0556960:CDS
TGTCCACCAAATATGGTGTA+CGG 0.595920 4_1:-55209587 None:intergenic
CTGATTCATGTGCTTTACCA+AGG 0.597601 4_1:+55206876 Msa0556960:CDS
GGGGAAGTTGAACCATTACC+GGG 0.597940 4_1:+55210315 Msa0556960:CDS
TCAAATTGAGCTTCAACGAA+GGG 0.606381 4_1:-55206800 None:intergenic
GTGGTGTAGGTTCAATGGAG+AGG 0.608923 4_1:+55207141 Msa0556960:intron
TTCATTGAGTCTAAACATGT+GGG 0.609808 4_1:+55207210 Msa0556960:CDS
ATCACATCGAGGGTAGGTGA+TGG 0.610525 4_1:-55206497 None:intergenic
TGTTGGTAGAGTGCTTACCC+TGG 0.618558 4_1:+55209623 Msa0556960:CDS
TGGACTTCAAATCACATCGA+GGG 0.620611 4_1:-55206507 None:intergenic
GAGATGGTTCTAATCTAGTG+AGG 0.627215 4_1:+55209995 Msa0556960:CDS
CTTCAAATCACATCGAGGGT+AGG 0.628501 4_1:-55206503 None:intergenic
AAGTATCAGGTAGTCCTGGT+AGG 0.628734 4_1:-55210512 None:intergenic
ATCATTTGCAGGAGGTAGAG+GGG 0.628843 4_1:-55210352 None:intergenic
TGGTAAAGCATGGGTAGACA+TGG 0.629944 4_1:+55209659 Msa0556960:CDS
AGATGGTTCTAATCTAGTGA+GGG 0.631565 4_1:+55209996 Msa0556960:CDS
TTCACTACATCATTTGCAGG+AGG 0.631894 4_1:-55210360 None:intergenic
TGGCACTGGTGGATTCACAC+TGG 0.642220 4_1:+55209702 Msa0556960:CDS
GAACCGAAGAAGACATGTAG+CGG 0.645973 4_1:+55210294 Msa0556960:CDS
CGGAAGAAGACAGTAGACAT+TGG 0.646035 4_1:-55207298 None:intergenic
ATTAACCCAACCAGCCCAAG+TGG 0.647017 4_1:-55210055 None:intergenic
CAGCTTGAAGAATCATAGAG+AGG 0.652645 4_1:-55206776 None:intergenic
TTCTAATCTAGTGAGGGACA+TGG 0.659068 4_1:+55210002 Msa0556960:CDS
TTCACACTGGAGATTTACGA+GGG 0.677263 4_1:+55209715 Msa0556960:CDS
ATTCACACTGGAGATTTACG+AGG 0.689675 4_1:+55209714 Msa0556960:CDS
ACCGAAGAAGACATGTAGCG+GGG 0.713042 4_1:+55210296 Msa0556960:CDS
GTGGACTTCAAATCACATCG+AGG 0.723756 4_1:-55206508 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGATTAATTTTTTAAGTT+TGG - chr4_1:55208037-55208056 None:intergenic 10.0%
!! CTTAAAAAATTAATCTTTAA+CGG + chr4_1:55208039-55208058 Msa0556960:intron 10.0%
!! ATTAATTCATTATAACATAT+GGG + chr4_1:55209284-55209303 Msa0556960:intron 10.0%
!! TCATTGATATTATTATATTA+GGG - chr4_1:55209470-55209489 None:intergenic 10.0%
!! ATCATTGATATTATTATATT+AGG - chr4_1:55209471-55209490 None:intergenic 10.0%
!!! AATAATTGTAATTTGTTCTT+TGG + chr4_1:55206728-55206747 Msa0556960:CDS 15.0%
!! TAAATGTAAAATGACATAAA+AGG + chr4_1:55208204-55208223 Msa0556960:intron 15.0%
!!! GGTTTTAATTAAAATGATAA+GGG + chr4_1:55208594-55208613 Msa0556960:intron 15.0%
!! CATTAATTCATTATAACATA+TGG + chr4_1:55209283-55209302 Msa0556960:intron 15.0%
!!! TATTTAAGATACTGATTATC+AGG - chr4_1:55206845-55206864 None:intergenic 20.0%
!! TATTTGAATAAGACTAGATT+AGG + chr4_1:55207614-55207633 Msa0556960:intron 20.0%
!!! AGATTATCTTTAAAAAAGTG+TGG + chr4_1:55207839-55207858 Msa0556960:intron 20.0%
!!! GATTATCTTTAAAAAAGTGT+GGG + chr4_1:55207840-55207859 Msa0556960:intron 20.0%
!!! GTTTGATCCTTTTAAAATTA+TGG - chr4_1:55208015-55208034 None:intergenic 20.0%
!!! AAAGTGTTTGGTAAAATTAA+CGG + chr4_1:55208168-55208187 Msa0556960:intron 20.0%
!! GTAAAGTATAAAAACTGAAA+TGG - chr4_1:55208353-55208372 None:intergenic 20.0%
!! AAGTGTGCTTTAATATATAA+AGG + chr4_1:55208562-55208581 Msa0556960:intron 20.0%
!! AGTGTGCTTTAATATATAAA+GGG + chr4_1:55208563-55208582 Msa0556960:intron 20.0%
!! TAATATATAAAGGGTAAATG+TGG + chr4_1:55208572-55208591 Msa0556960:intron 20.0%
!! AATATATAAAGGGTAAATGT+GGG + chr4_1:55208573-55208592 Msa0556960:intron 20.0%
!!! GGGTTTTAATTAAAATGATA+AGG + chr4_1:55208593-55208612 Msa0556960:intron 20.0%
!! ATATTAGGGATATAAATGAT+CGG - chr4_1:55209456-55209475 None:intergenic 20.0%
!! TTCAAATATACATTCAATTG+TGG - chr4_1:55209780-55209799 None:intergenic 20.0%
!! TTGAATGTATATTTGAATTG+AGG + chr4_1:55209783-55209802 Msa0556960:intron 20.0%
!!! TTCACTCTAATTAAAAGTAT+TGG - chr4_1:55210173-55210192 None:intergenic 20.0%
!! TGTGAAATTAGAGTATATAT+TGG + chr4_1:55210565-55210584 Msa0556960:exon 20.0%
!! ATATTCTCTTTCACAGTTTT+TGG + chr4_1:55206596-55206615 Msa0556960:five_prime_UTR 25.0%
! CAATTTGATTGCCAAAAATA+TGG + chr4_1:55206815-55206834 Msa0556960:CDS 25.0%
! ATCAGTATCTTAAATACTCT+TGG + chr4_1:55206849-55206868 Msa0556960:CDS 25.0%
! TATACAAACTAACAATACCT+TGG - chr4_1:55206896-55206915 None:intergenic 25.0%
! TATGTGACATATCACAAAAA+GGG - chr4_1:55206938-55206957 None:intergenic 25.0%
! ATATGTGACATATCACAAAA+AGG - chr4_1:55206939-55206958 None:intergenic 25.0%
!! TAAACATGTTTTCCTTTCTA+GGG - chr4_1:55207021-55207040 None:intergenic 25.0%
!! ATAAACATGTTTTCCTTTCT+AGG - chr4_1:55207022-55207041 None:intergenic 25.0%
! TTACATTTGTACTAATCGTT+AGG - chr4_1:55207106-55207125 None:intergenic 25.0%
! GATAAGAAGATAATGTTTGT+GGG + chr4_1:55207184-55207203 Msa0556960:CDS 25.0%
!!! CATTTTGTTTGTTTCTTTTG+CGG + chr4_1:55207410-55207429 Msa0556960:intron 25.0%
!!! ATTTTGTTTGTTTCTTTTGC+GGG + chr4_1:55207411-55207430 Msa0556960:intron 25.0%
!!! TAACTACTTTTAGAAAGAGA+GGG - chr4_1:55207443-55207462 None:intergenic 25.0%
!!! ATAACTACTTTTAGAAAGAG+AGG - chr4_1:55207444-55207463 None:intergenic 25.0%
! CTTGTTACATGTAAAATGAT+AGG + chr4_1:55207493-55207512 Msa0556960:intron 25.0%
! AATTTCGAGCTTAATATCTT+TGG - chr4_1:55207552-55207571 None:intergenic 25.0%
! AATATCTCTATGGAAAATGA+TGG - chr4_1:55207598-55207617 None:intergenic 25.0%
!! GTCTTATTCAAATATCTCTA+TGG - chr4_1:55207608-55207627 None:intergenic 25.0%
! ACTATATTATGCATGTCAAA+CGG + chr4_1:55207763-55207782 Msa0556960:intron 25.0%
! AATATCTAGATTTAGTGTTG+AGG - chr4_1:55207899-55207918 None:intergenic 25.0%
!!! CACAAAATAAGCTTTTAAAG+AGG + chr4_1:55207977-55207996 Msa0556960:intron 25.0%
!!! TTTTAAAGAGGCTATAGATT+GGG + chr4_1:55207989-55208008 Msa0556960:intron 25.0%
!! GATAAGCTAATTAAAGTGTT+TGG + chr4_1:55208156-55208175 Msa0556960:intron 25.0%
! TTTGAAAACGTGATATACAT+AGG + chr4_1:55208485-55208504 Msa0556960:intron 25.0%
! TAAAATGATAAGGGTAAAAG+TGG + chr4_1:55208603-55208622 Msa0556960:intron 25.0%
!! TGATAAAAAATAGCTCTGTT+TGG - chr4_1:55208742-55208761 None:intergenic 25.0%
!!! AAAAGCTAAAAGCTCTTTTT+TGG + chr4_1:55208785-55208804 Msa0556960:intron 25.0%
!! ATGCTTCAATTTTTCTAACT+AGG + chr4_1:55208845-55208864 Msa0556960:intron 25.0%
! TTGTAAATTGTTGCAATCAT+AGG - chr4_1:55208944-55208963 None:intergenic 25.0%
! AAATTTGCATTGTCATTACA+TGG + chr4_1:55209141-55209160 Msa0556960:intron 25.0%
! AATTTGCATTGTCATTACAT+GGG + chr4_1:55209142-55209161 Msa0556960:intron 25.0%
! ATGAAATTATGACACTACTT+TGG + chr4_1:55209323-55209342 Msa0556960:intron 25.0%
! TGAAATTATGACACTACTTT+GGG + chr4_1:55209324-55209343 Msa0556960:intron 25.0%
!! AAAGTGATGAATGTGTTATT+TGG + chr4_1:55209944-55209963 Msa0556960:intron 25.0%
!!! AGAAGAGTTTAGTTTTAGTA+GGG - chr4_1:55210092-55210111 None:intergenic 25.0%
!!! AAGAAGAGTTTAGTTTTAGT+AGG - chr4_1:55210093-55210112 None:intergenic 25.0%
! AAAACTAAACTCTTCTTTCA+AGG + chr4_1:55210096-55210115 Msa0556960:CDS 25.0%
!!! TTACTTACTTGTTATGAGTA+GGG - chr4_1:55210128-55210147 None:intergenic 25.0%
!! TTTTCGTAAATGTTCTTAAG+AGG + chr4_1:55210677-55210696 Msa0556960:three_prime_UTR 25.0%
!! TAATTTGTTCTTTGGAAGCT+GGG + chr4_1:55206736-55206755 Msa0556960:CDS 30.0%
! TATCAGGTCTTCCATATTTT+TGG - chr4_1:55206829-55206848 None:intergenic 30.0%
AATTTCATGTTTCCCTAGAA+AGG + chr4_1:55207006-55207025 Msa0556960:intron 30.0%
GTAATGAGTAATCATTTCTG+TGG + chr4_1:55207122-55207141 Msa0556960:intron 30.0%
!!! ATGGAGAGGATTTTTTGAAT+AGG + chr4_1:55207155-55207174 Msa0556960:CDS 30.0%
GGATAAGAAGATAATGTTTG+TGG + chr4_1:55207183-55207202 Msa0556960:CDS 30.0%
ATTCATTGAGTCTAAACATG+TGG + chr4_1:55207209-55207228 Msa0556960:CDS 30.0%
TTCATTGAGTCTAAACATGT+GGG + chr4_1:55207210-55207229 Msa0556960:CDS 30.0%
AAACAAGAACAACTATTACC+CGG - chr4_1:55207321-55207340 None:intergenic 30.0%
AAACAAAATGATGACACACA+AGG - chr4_1:55207400-55207419 None:intergenic 30.0%
GATATTAAGCTCGAAATTGA+AGG + chr4_1:55207554-55207573 Msa0556960:intron 30.0%
ATTAGGTTATAGACTCTTGT+CGG - chr4_1:55207644-55207663 None:intergenic 30.0%
!! CTTTTAAAGAGGCTATAGAT+TGG + chr4_1:55207988-55208007 Msa0556960:intron 30.0%
! GATTGGGCCATAATTTTAAA+AGG + chr4_1:55208005-55208024 Msa0556960:intron 30.0%
CTATTACATGTTACTCTGTT+TGG - chr4_1:55208816-55208835 None:intergenic 30.0%
! CAGTCAGACTTTGTTTAAAT+AGG - chr4_1:55208882-55208901 None:intergenic 30.0%
GATTGCAACAATTTACAAGT+TGG + chr4_1:55208946-55208965 Msa0556960:intron 30.0%
! AGTTCTTCGTAATTTTCTTG+CGG - chr4_1:55209379-55209398 None:intergenic 30.0%
TATAAATGATCGGATATCCA+CGG - chr4_1:55209446-55209465 None:intergenic 30.0%
TGAATTATGTCCACCAAATA+TGG - chr4_1:55209597-55209616 None:intergenic 30.0%
GACATAATTCAAGAAGATGT+TGG + chr4_1:55209606-55209625 Msa0556960:CDS 30.0%
TATAAAAGCTGGTAAAGCAT+GGG + chr4_1:55209650-55209669 Msa0556960:CDS 30.0%
ATTTGAATTGAGGTGATTTG+AGG + chr4_1:55209793-55209812 Msa0556960:intron 30.0%
CCTTATAAAATGCATCCAAA+CGG - chr4_1:55210029-55210048 None:intergenic 30.0%
! CGTTTGGATGCATTTTATAA+GGG + chr4_1:55210027-55210046 Msa0556960:CDS 30.0%
!! TACTTACTTGTTATGAGTAG+GGG - chr4_1:55210127-55210146 None:intergenic 30.0%
!! GTTACTTACTTGTTATGAGT+AGG - chr4_1:55210129-55210148 None:intergenic 30.0%
TGGTGTAAATGAGCATATAT+TGG - chr4_1:55210153-55210172 None:intergenic 30.0%
ACATCCTTCTATCTATACTA+AGG + chr4_1:55210458-55210477 Msa0556960:CDS 30.0%
! TGTGATTTGAAGTCCACATA+TGG + chr4_1:55206514-55206533 Msa0556960:five_prime_UTR 35.0%
AGAAAAACTAGTGCCATATG+TGG - chr4_1:55206530-55206549 None:intergenic 35.0%
!! AGTTTTTGGACCACCAAAAT+AGG + chr4_1:55206610-55206629 Msa0556960:five_prime_UTR 35.0%
! AAAGCCATATGGTCCTATTT+TGG - chr4_1:55206626-55206645 None:intergenic 35.0%
TATGGACATTGAAAGCCATA+TGG - chr4_1:55206637-55206656 None:intergenic 35.0%
GAGAGAGCAAAAACAATGTA+TGG - chr4_1:55206655-55206674 None:intergenic 35.0%
GCATGGATATAAGGAGATTT+AGG - chr4_1:55206685-55206704 None:intergenic 35.0%
!! GTAATTTGTTCTTTGGAAGC+TGG + chr4_1:55206735-55206754 Msa0556960:CDS 35.0%
TCAAATTGAGCTTCAACGAA+GGG - chr4_1:55206803-55206822 None:intergenic 35.0%
ATCAAATTGAGCTTCAACGA+AGG - chr4_1:55206804-55206823 None:intergenic 35.0%
AGTAATCATTTCTGTGGTGT+AGG + chr4_1:55207128-55207147 Msa0556960:intron 35.0%
!!! GAGAGGATTTTTTGAATAGG+TGG + chr4_1:55207158-55207177 Msa0556960:CDS 35.0%
!!! GGATTTTTTGAATAGGTGGA+AGG + chr4_1:55207162-55207181 Msa0556960:CDS 35.0%
GAAAAGCTTGTAGTGACATT+CGG - chr4_1:55207268-55207287 None:intergenic 35.0%
! ATGTCACTACAAGCTTTTCT+AGG + chr4_1:55207269-55207288 Msa0556960:CDS 35.0%
TTAGGTTATAGACTCTTGTC+GGG - chr4_1:55207643-55207662 None:intergenic 35.0%
AACCTAATCTACACAAAGTC+TGG + chr4_1:55207656-55207675 Msa0556960:intron 35.0%
AACCAGACTTTGTGTAGATT+AGG - chr4_1:55207661-55207680 None:intergenic 35.0%
! CGTGACTTCTACTTTGAAAA+AGG - chr4_1:55207936-55207955 None:intergenic 35.0%
TAATCTTTAACGGCTCTGTT+TGG + chr4_1:55208049-55208068 Msa0556960:intron 35.0%
GCTCTGTTTGGTAAAAATAG+CGG + chr4_1:55208061-55208080 Msa0556960:intron 35.0%
AACGTGATATACATAGGAAC+TGG + chr4_1:55208491-55208510 Msa0556960:intron 35.0%
TACATAGGAACTGGAAAACT+AGG + chr4_1:55208500-55208519 Msa0556960:intron 35.0%
ACGAGCTTATAGCTTATTTC+AGG - chr4_1:55208704-55208723 None:intergenic 35.0%
TGTGCAATCAACATCAATCA+TGG + chr4_1:55209002-55209021 Msa0556960:intron 35.0%
GTGCAATCAACATCAATCAT+GGG + chr4_1:55209003-55209022 Msa0556960:intron 35.0%
CTACTATATATCCATATCCG+TGG + chr4_1:55209426-55209445 Msa0556960:intron 35.0%
! CACTACCACAGTTTCATTTT+GGG - chr4_1:55209520-55209539 None:intergenic 35.0%
! TCACTACCACAGTTTCATTT+TGG - chr4_1:55209521-55209540 None:intergenic 35.0%
ACTATACCGTACACCATATT+TGG + chr4_1:55209581-55209600 Msa0556960:CDS 35.0%
CTATAAAAGCTGGTAAAGCA+TGG + chr4_1:55209649-55209668 Msa0556960:CDS 35.0%
!! TTATTTGGCTTGCTTCATTC+AGG + chr4_1:55209959-55209978 Msa0556960:intron 35.0%
!! AGATGGTTCTAATCTAGTGA+GGG + chr4_1:55209996-55210015 Msa0556960:CDS 35.0%
! CCGTTTGGATGCATTTTATA+AGG + chr4_1:55210026-55210045 Msa0556960:CDS 35.0%
TTTATAAGGGCTTAACCACT+TGG + chr4_1:55210040-55210059 Msa0556960:CDS 35.0%
TTATAAGGGCTTAACCACTT+GGG + chr4_1:55210041-55210060 Msa0556960:CDS 35.0%
TTGTTCACTACATCATTTGC+AGG - chr4_1:55210366-55210385 None:intergenic 35.0%
GTTAAAGAACATGAAGAAGC+AGG + chr4_1:55210389-55210408 Msa0556960:CDS 35.0%
GGATCCTTAGTATAGATAGA+AGG - chr4_1:55210465-55210484 None:intergenic 35.0%
ATCTATACTAAGGATCCAAC+TGG + chr4_1:55210468-55210487 Msa0556960:CDS 35.0%
ACATGATTAGAGCTGCATTT+AGG - chr4_1:55210548-55210567 None:intergenic 35.0%
TGGACTTCAAATCACATCGA+GGG - chr4_1:55206510-55206529 None:intergenic 40.0%
ACCACCAAAATAGGACCATA+TGG + chr4_1:55206619-55206638 Msa0556960:exon 40.0%
! TTGACTTTTGTGCTCTTGCT+GGG - chr4_1:55206709-55206728 None:intergenic 40.0%
!! TTTGACTTTTGTGCTCTTGC+TGG - chr4_1:55206710-55206729 None:intergenic 40.0%
CAGCTTGAAGAATCATAGAG+AGG - chr4_1:55206779-55206798 None:intergenic 40.0%
CTTGGTAAAGCACATGAATC+AGG - chr4_1:55206878-55206897 None:intergenic 40.0%
CTGATTCATGTGCTTTACCA+AGG + chr4_1:55206876-55206895 Msa0556960:CDS 40.0%
TTTCTGTGGTGTAGGTTCAA+TGG + chr4_1:55207136-55207155 Msa0556960:intron 40.0%
GTCTTGTATGATTCATGCGT+CGG + chr4_1:55207240-55207259 Msa0556960:CDS 40.0%
CAATGTCTACTGTCTTCTTC+CGG + chr4_1:55207299-55207318 Msa0556960:CDS 40.0%
AATGTCTACTGTCTTCTTCC+GGG + chr4_1:55207300-55207319 Msa0556960:CDS 40.0%
TGTCTTAGACTCACACACAT+AGG + chr4_1:55207343-55207362 Msa0556960:intron 40.0%
CATCAATCATGGGTATGCTT+TGG + chr4_1:55209013-55209032 Msa0556960:intron 40.0%
AACCACCCAAAATGAAACTG+TGG + chr4_1:55209512-55209531 Msa0556960:intron 40.0%
! TACCACAGTTTCATTTTGGG+TGG - chr4_1:55209517-55209536 None:intergenic 40.0%
! GAAACTGTGGTAGTGATACT+TGG + chr4_1:55209525-55209544 Msa0556960:intron 40.0%
TGGTGTACGGTATAGTTGTA+TGG - chr4_1:55209577-55209596 None:intergenic 40.0%
ATACCGTACACCATATTTGG+TGG + chr4_1:55209584-55209603 Msa0556960:CDS 40.0%
TGTCCACCAAATATGGTGTA+CGG - chr4_1:55209590-55209609 None:intergenic 40.0%
! ACCAGCTTTTATAGAGTCCA+GGG - chr4_1:55209643-55209662 None:intergenic 40.0%
! TACCAGCTTTTATAGAGTCC+AGG - chr4_1:55209644-55209663 None:intergenic 40.0%
ACATGTTGATCTTCAACTCC+TGG + chr4_1:55209682-55209701 Msa0556960:CDS 40.0%
ATTCACACTGGAGATTTACG+AGG + chr4_1:55209714-55209733 Msa0556960:CDS 40.0%
TTCACACTGGAGATTTACGA+GGG + chr4_1:55209715-55209734 Msa0556960:CDS 40.0%
GAGGTGATTTGAGGAAACTA+CGG + chr4_1:55209802-55209821 Msa0556960:intron 40.0%
! AGGTGGGATTTTATCAGAGA+TGG + chr4_1:55209979-55209998 Msa0556960:intron 40.0%
!! GAGATGGTTCTAATCTAGTG+AGG + chr4_1:55209995-55210014 Msa0556960:CDS 40.0%
TTCTAATCTAGTGAGGGACA+TGG + chr4_1:55210002-55210021 Msa0556960:CDS 40.0%
! CTTTTCTCCTTGAATCCAGA+GGG + chr4_1:55210259-55210278 Msa0556960:intron 40.0%
TTCACTACATCATTTGCAGG+AGG - chr4_1:55210363-55210382 None:intergenic 40.0%
TGACTACAATCAATGCCAGT+TGG - chr4_1:55210486-55210505 None:intergenic 40.0%
!! CTGGCATTGATTGTAGTCAT+TGG + chr4_1:55210487-55210506 Msa0556960:CDS 40.0%
! AGGAGTATGTTCCAAGTATC+AGG - chr4_1:55210528-55210547 None:intergenic 40.0%
CTTCAAATCACATCGAGGGT+AGG - chr4_1:55206506-55206525 None:intergenic 45.0%
GTGGACTTCAAATCACATCG+AGG - chr4_1:55206511-55206530 None:intergenic 45.0%
!! GCCATATGGTCCTATTTTGG+TGG - chr4_1:55206623-55206642 None:intergenic 45.0%
TTGCTGGGTGCATGGATATA+AGG - chr4_1:55206694-55206713 None:intergenic 45.0%
CGGAAGAAGACAGTAGACAT+TGG - chr4_1:55207301-55207320 None:intergenic 45.0%
TCTCGCTTGAGGGTGATAAT+AGG - chr4_1:55208912-55208931 None:intergenic 45.0%
TATTATCACCCTCAAGCGAG+AGG + chr4_1:55208911-55208930 Msa0556960:intron 45.0%
TGATCGGATATCCACGGATA+TGG - chr4_1:55209440-55209459 None:intergenic 45.0%
! GATACTTGGTGATATTGCGC+AGG + chr4_1:55209539-55209558 Msa0556960:intron 45.0%
ACCCTGGACTCTATAAAAGC+TGG + chr4_1:55209639-55209658 Msa0556960:CDS 45.0%
TGGTAAAGCATGGGTAGACA+TGG + chr4_1:55209659-55209678 Msa0556960:CDS 45.0%
!! TTTGGCTTGCTTCATTCAGG+TGG + chr4_1:55209962-55209981 Msa0556960:intron 45.0%
!! TTGGCTTGCTTCATTCAGGT+GGG + chr4_1:55209963-55209982 Msa0556960:intron 45.0%
CCTCTGGATTCAAGGAGAAA+AGG - chr4_1:55210261-55210280 None:intergenic 45.0%
! CCTTTTCTCCTTGAATCCAG+AGG + chr4_1:55210258-55210277 Msa0556960:intron 45.0%
GAACCGAAGAAGACATGTAG+CGG + chr4_1:55210294-55210313 Msa0556960:CDS 45.0%
AACCGAAGAAGACATGTAGC+GGG + chr4_1:55210295-55210314 Msa0556960:CDS 45.0%
ATTACCGGGAACCACATATC+CGG + chr4_1:55210329-55210348 Msa0556960:CDS 45.0%
ATCATTTGCAGGAGGTAGAG+GGG - chr4_1:55210355-55210374 None:intergenic 45.0%
CATCATTTGCAGGAGGTAGA+GGG - chr4_1:55210356-55210375 None:intergenic 45.0%
ACATCATTTGCAGGAGGTAG+AGG - chr4_1:55210357-55210376 None:intergenic 45.0%
AAGTATCAGGTAGTCCTGGT+AGG - chr4_1:55210515-55210534 None:intergenic 45.0%
TACCAGGACTACCTGATACT+TGG + chr4_1:55210514-55210533 Msa0556960:CDS 45.0%
TTCCAAGTATCAGGTAGTCC+TGG - chr4_1:55210519-55210538 None:intergenic 45.0%
!! ATATTATATAATAATATCAA+AGG + chr4_1:55207813-55207832 Msa0556960:intron 5.0%
!! AATTTAATTACTATATATAT+TGG + chr4_1:55210646-55210665 Msa0556960:three_prime_UTR 5.0%
ATCACATCGAGGGTAGGTGA+TGG - chr4_1:55206500-55206519 None:intergenic 50.0%
GTGGTGTAGGTTCAATGGAG+AGG + chr4_1:55207141-55207160 Msa0556960:intron 50.0%
GTTGACATCCTCTCGCTTGA+GGG - chr4_1:55208922-55208941 None:intergenic 50.0%
GGTGATATTGCGCAGGACTA+TGG + chr4_1:55209546-55209565 Msa0556960:intron 50.0%
! TGTTGGTAGAGTGCTTACCC+TGG + chr4_1:55209623-55209642 Msa0556960:CDS 50.0%
TGATCTTCAACTCCTGGCAC+TGG + chr4_1:55209688-55209707 Msa0556960:CDS 50.0%
!! CTTAACCACTTGGGCTGGTT+GGG + chr4_1:55210050-55210069 Msa0556960:CDS 50.0%
ATTAACCCAACCAGCCCAAG+TGG - chr4_1:55210058-55210077 None:intergenic 50.0%
AATCCTGCCCTCTGGATTCA+AGG - chr4_1:55210269-55210288 None:intergenic 50.0%
TTGAATCCAGAGGGCAGGAT+TGG + chr4_1:55210268-55210287 Msa0556960:intron 50.0%
TTCATTCCAATCCTGCCCTC+TGG - chr4_1:55210277-55210296 None:intergenic 50.0%
ACCGAAGAAGACATGTAGCG+GGG + chr4_1:55210296-55210315 Msa0556960:CDS 50.0%
TCCCCGCTACATGTCTTCTT+CGG - chr4_1:55210300-55210319 None:intergenic 50.0%
CGGGGAAGTTGAACCATTAC+CGG + chr4_1:55210314-55210333 Msa0556960:CDS 50.0%
GGGGAAGTTGAACCATTACC+GGG + chr4_1:55210315-55210334 Msa0556960:CDS 50.0%
GGATATGTGGTTCCCGGTAA+TGG - chr4_1:55210330-55210349 None:intergenic 50.0%
! TGTAGTCATTGGTGCCTACC+AGG + chr4_1:55210498-55210517 Msa0556960:CDS 50.0%
TTGTGCTCTTGCTGGGTGCA+TGG - chr4_1:55206702-55206721 None:intergenic 55.0%
GGTTGACATCCTCTCGCTTG+AGG - chr4_1:55208923-55208942 None:intergenic 55.0%
! TCTTCAACTCCTGGCACTGG+TGG + chr4_1:55209691-55209710 Msa0556960:CDS 55.0%
AGTGTGAATCCACCAGTGCC+AGG - chr4_1:55209703-55209722 None:intergenic 55.0%
!! TGGCACTGGTGGATTCACAC+TGG + chr4_1:55209702-55209721 Msa0556960:CDS 55.0%
AGTGAGGGACATGGACCGTT+TGG + chr4_1:55210011-55210030 Msa0556960:CDS 55.0%
AAGGGCTTAACCACTTGGGC+TGG + chr4_1:55210045-55210064 Msa0556960:CDS 55.0%
!! GCTTAACCACTTGGGCTGGT+TGG + chr4_1:55210049-55210068 Msa0556960:CDS 55.0%
TCTCCTTGAATCCAGAGGGC+AGG + chr4_1:55210263-55210282 Msa0556960:intron 55.0%
AGGTAGAGGGGCCGGATATG+TGG - chr4_1:55210343-55210362 None:intergenic 60.0%
TTTGCAGGAGGTAGAGGGGC+CGG - chr4_1:55210351-55210370 None:intergenic 60.0%
GGGGCCGGATATGTGGTTCC+CGG - chr4_1:55210336-55210355 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 55206482 55210721 55206482 ID=Msa0556960;Name=Msa0556960
chr4_1 mRNA 55206482 55210721 55206482 ID=Msa0556960-mRNA-1;Parent=Msa0556960;Name=Msa0556960-mRNA-1;_AED=0.13;_eAED=0.13;_QI=156|1|1|1|0.8|0.66|6|127|352
chr4_1 exon 55206482 55206897 55206482 ID=Msa0556960-mRNA-1:exon:16448;Parent=Msa0556960-mRNA-1
chr4_1 exon 55207150 55207321 55207150 ID=Msa0556960-mRNA-1:exon:16449;Parent=Msa0556960-mRNA-1
chr4_1 exon 55209561 55209736 55209561 ID=Msa0556960-mRNA-1:exon:16450;Parent=Msa0556960-mRNA-1
chr4_1 exon 55209981 55210139 55209981 ID=Msa0556960-mRNA-1:exon:16451;Parent=Msa0556960-mRNA-1
chr4_1 exon 55210278 55210585 55210278 ID=Msa0556960-mRNA-1:exon:16452;Parent=Msa0556960-mRNA-1
chr4_1 exon 55210611 55210721 55210611 ID=Msa0556960-mRNA-1:exon:16453;Parent=Msa0556960-mRNA-1
chr4_1 five_prime_UTR 55206482 55206637 55206482 ID=Msa0556960-mRNA-1:five_prime_utr;Parent=Msa0556960-mRNA-1
chr4_1 CDS 55206638 55206897 55206638 ID=Msa0556960-mRNA-1:cds;Parent=Msa0556960-mRNA-1
chr4_1 CDS 55207150 55207321 55207150 ID=Msa0556960-mRNA-1:cds;Parent=Msa0556960-mRNA-1
chr4_1 CDS 55209561 55209736 55209561 ID=Msa0556960-mRNA-1:cds;Parent=Msa0556960-mRNA-1
chr4_1 CDS 55209981 55210139 55209981 ID=Msa0556960-mRNA-1:cds;Parent=Msa0556960-mRNA-1
chr4_1 CDS 55210278 55210569 55210278 ID=Msa0556960-mRNA-1:cds;Parent=Msa0556960-mRNA-1
chr4_1 three_prime_UTR 55210570 55210585 55210570 ID=Msa0556960-mRNA-1:three_prime_utr;Parent=Msa0556960-mRNA-1
chr4_1 three_prime_UTR 55210611 55210721 55210611 ID=Msa0556960-mRNA-1:three_prime_utr;Parent=Msa0556960-mRNA-1
Gene Sequence

>Msa0556960

ATGGCTTTCAATGTCCATACATTGTTTTTGCTCTCTCTGTTTAGCCTAAATCTCCTTATATCCATGCACCCAGCAAGAGCACAAAAGTCAAATAATTGTAATTTGTTCTTTGGAAGCTGGGTGATTGACTCTTCATATCCTCTCTATGATTCTTCAAGCTGTCCCTTCGTTGAAGCTCAATTTGATTGCCAAAAATATGGAAGACCTGATAATCAGTATCTTAAATACTCTTGGAAGCCTGATTCATGTGCTTTACCAAGGTTCAATGGAGAGGATTTTTTGAATAGGTGGAAGGATAAGAAGATAATGTTTGTGGGTGATTCATTGAGTCTAAACATGTGGGAATCATTGTCTTGTATGATTCATGCGTCGGTTCCGAATGTCACTACAAGCTTTTCTAGGAAAGAACCAATGTCTACTGTCTTCTTCCGGGACTATGGAGTAACCATACAACTATACCGTACACCATATTTGGTGGACATAATTCAAGAAGATGTTGGTAGAGTGCTTACCCTGGACTCTATAAAAGCTGGTAAAGCATGGGTAGACATGGACATGTTGATCTTCAACTCCTGGCACTGGTGGATTCACACTGGAGATTTACGAGGGTGGGATTTTATCAGAGATGGTTCTAATCTAGTGAGGGACATGGACCGTTTGGATGCATTTTATAAGGGCTTAACCACTTGGGCTGGTTGGGTTAATGCAAATGTTGACCCTACTAAAACTAAACTCTTCTTTCAAGGAATTTCCCCTACTCATAACAAAGGGCAGGATTGGAATGAACCGAAGAAGACATGTAGCGGGGAAGTTGAACCATTACCGGGAACCACATATCCGGCCCCTCTACCTCCTGCAAATGATGTAGTGAACAAAGTGTTAAAGAACATGAAGAAGCAGGTTTATCTACTTGACATAACACTTCTCTCACAACTAAGAAAAGATGCACATCCTTCTATCTATACTAAGGATCCAACTGGCATTGATTGTAGTCATTGGTGCCTACCAGGACTACCTGATACTTGGAACATACTCCTAAATGCAGCTCTAATCATGTGA
Protein sequence

>Msa0556960

MAFNVHTLFLLSLFSLNLLISMHPARAQKSNNCNLFFGSWVIDSSYPLYDSSSCPFVEAQFDCQKYGRPDNQYLKYSWKPDSCALPRFNGEDFLNRWKDKKIMFVGDSLSLNMWESLSCMIHASVPNVTTSFSRKEPMSTVFFRDYGVTIQLYRTPYLVDIIQEDVGRVLTLDSIKAGKAWVDMDMLIFNSWHWWIHTGDLRGWDFIRDGSNLVRDMDRLDAFYKGLTTWAGWVNANVDPTKTKLFFQGISPTHNKGQDWNEPKKTCSGEVEPLPGTTYPAPLPPANDVVNKVLKNMKKQVYLLDITLLSQLRKDAHPSIYTKDPTGIDCSHWCLPGLPDTWNILLNAALIM*