AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026299.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026299.01.T01 MTR_5g043620 91.822 269 16 3 1 269 1 263 8.88e-179 496
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026299.01.T01 AT4G30580 67.500 200 58 3 73 269 92 287 1.28e-94 283

Find 61 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACATTTCTTTGCTTATTT+TGG 0.064584 5:+34332574 None:intergenic
TCCACACAAGTCGTTGAATT+TGG 0.150006 5:+34332533 None:intergenic
CTTAATTGCCTCTTCTTCTA+TGG 0.199821 5:+34332999 None:intergenic
TGTTCCACGTAATCCAATTC+TGG 0.260445 5:+34332844 None:intergenic
TGAAACAATTGCAGAATAAA+AGG 0.278366 5:+34332934 None:intergenic
CCCAACCAATAATCGGAAAA+AGG 0.278921 5:+34332058 None:intergenic
CGTACTAAAGATGGAAAATT+AGG 0.280561 5:-34331816 MsG0580026299.01.T01:CDS
ACATAGACGGCTGGAGTATC+TGG 0.283495 5:+34332165 None:intergenic
TGGTTGGGCAATGTTTCTTT+TGG 0.292120 5:-34332043 MsG0580026299.01.T01:CDS
GGTTAGTTGGATTGTATCTA+TGG 0.311817 5:+34332971 None:intergenic
CCACACAAGTCGTTGAATTT+GGG 0.325398 5:+34332534 None:intergenic
ATTACTTTGTCGCCAAACTC+TGG 0.335764 5:-34332251 MsG0580026299.01.T01:CDS
GACTTGTGTGGAACTGGAAT+TGG 0.369457 5:-34332522 MsG0580026299.01.T01:CDS
TCTCGCCAACAACCCAGAAT+TGG 0.370143 5:-34332857 MsG0580026299.01.T01:CDS
CAATTTGTTGTAACAGAAAC+GGG 0.371977 5:+34332494 None:intergenic
TTTAAATTCATAAGCAAGAC+TGG 0.379901 5:-34332087 MsG0580026299.01.T01:CDS
ATCTAGAAAACTCTGATGAT+TGG 0.382445 5:+34332140 None:intergenic
TTCAACGACTTGTGTGGAAC+TGG 0.388456 5:-34332528 MsG0580026299.01.T01:CDS
ATTGCAGAATAAAAGGTAAA+CGG 0.406600 5:+34332941 None:intergenic
TGGAAAATTAGGTGCATTCA+AGG 0.408568 5:-34331805 MsG0580026299.01.T01:CDS
AACATTGCCCAACCAATAAT+CGG 0.408597 5:+34332051 None:intergenic
AGTGAGTGTAGCCCAGAGTT+TGG 0.422387 5:+34332239 None:intergenic
CGATCAACAAGGAGCACAAA+TGG 0.428683 5:+34332291 None:intergenic
ATTTGGGTTCAAGTATTGAA+TGG 0.431128 5:+34332400 None:intergenic
TCTTTAGTACGTGTTCCCTC+CGG 0.433745 5:+34331828 None:intergenic
TCAATTTGTTGTAACAGAAA+CGG 0.436103 5:+34332493 None:intergenic
TATGAGATTACTGCTGCAAC+AGG 0.436954 5:-34332438 MsG0580026299.01.T01:CDS
CCAAATTCGAAGCAAAACTT+AGG 0.437800 5:-34332383 MsG0580026299.01.T01:CDS
CAAAGTAATGAAACTTTCTT+CGG 0.450888 5:+34332265 None:intergenic
AAACGGGAAAGAAGGTTAGT+TGG 0.455879 5:+34332958 None:intergenic
TTGCAGAATAAAAGGTAAAC+GGG 0.457813 5:+34332942 None:intergenic
ATATATACTCTTCTTACGCT+TGG 0.460722 5:-34332117 MsG0580026299.01.T01:CDS
CAAAATTGAATATCAAGGCT+TGG 0.461201 5:-34332202 MsG0580026299.01.T01:CDS
GTAATCCAATTCTGGGTTGT+TGG 0.462779 5:+34332852 None:intergenic
GTCGTTGAATTTGGGAGTAA+AGG 0.464488 5:+34332542 None:intergenic
TTTGGGTTCAAGTATTGAAT+GGG 0.474415 5:+34332401 None:intergenic
GTTCCACGTAATCCAATTCT+GGG 0.474871 5:+34332845 None:intergenic
CGACTGTCCATGCGCTTCAA+AGG 0.487372 5:+34332012 None:intergenic
CTTTAGTACGTGTTCCCTCC+GGG 0.487779 5:+34331829 None:intergenic
TTACTTTGTCGCCAAACTCT+GGG 0.493014 5:-34332250 MsG0580026299.01.T01:CDS
TAAAAGGTAAACGGGAAAGA+AGG 0.504754 5:+34332950 None:intergenic
CAAATTCGAAGCAAAACTTA+GGG 0.510545 5:-34332382 MsG0580026299.01.T01:CDS
TAGAGACAACGATGATGGAT+TGG 0.518714 5:+34332890 None:intergenic
GAGGGAACACGTACTAAAGA+TGG 0.519060 5:-34331825 MsG0580026299.01.T01:CDS
CAACGATTCCATAGAAGAAG+AGG 0.525945 5:-34333007 MsG0580026299.01.T01:five_prime_UTR
TGTAATTCCTTTGAAGCGCA+TGG 0.527397 5:-34332019 MsG0580026299.01.T01:CDS
AGAGATAGAGACAACGATGA+TGG 0.529836 5:+34332885 None:intergenic
TTATCTTATCTACTCTTCAA+AGG 0.539713 5:+34331758 None:intergenic
CCCAAATTCAACGACTTGTG+TGG 0.542928 5:-34332534 MsG0580026299.01.T01:CDS
GATTGGAGAAGCAACTTCCA+TGG 0.555134 5:+34332907 None:intergenic
CAACCCAGAATTGGATTACG+TGG 0.555211 5:-34332848 MsG0580026299.01.T01:CDS
ATTACTGCTGCAACAGGAGA+CGG 0.572685 5:-34332432 MsG0580026299.01.T01:CDS
TGATTGGAAACATAGACGGC+TGG 0.595089 5:+34332156 None:intergenic
CATGGACAGTCGAAGCCAGA+TGG 0.596435 5:-34332001 MsG0580026299.01.T01:intron
CTGATGATTGGAAACATAGA+CGG 0.597420 5:+34332152 None:intergenic
ACGGCTGGAGTATCTGGAGA+TGG 0.617416 5:+34332171 None:intergenic
TTAAATTCATAAGCAAGACT+GGG 0.620552 5:-34332086 MsG0580026299.01.T01:CDS
ATAAGCAAAGAAATGTGTTG+AGG 0.633011 5:-34332569 MsG0580026299.01.T01:CDS
AAATTCGAAGCAAAACTTAG+GGG 0.693042 5:-34332381 MsG0580026299.01.T01:CDS
ATCTTACAATGACATCTCTG+CGG 0.702894 5:+34332460 None:intergenic
TTCTTCGGTAACGATCAACA+AGG 0.732934 5:+34332280 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCAAAAAAAAAAAAAAACAA+TGG + Chr5:34332843-34332862 None:intergenic 15.0%
!!! TGATATTCAATTTTGAAAAA+TGG + Chr5:34332586-34332605 None:intergenic 15.0%
!!! TGATTTTATCAAATTAATAG+TGG + Chr5:34332050-34332069 None:intergenic 15.0%
!!! TTTTTCAAAATTGAATATCA+AGG - Chr5:34332586-34332605 MsG0580026299.01.T01:CDS 15.0%
!! AAAAAGAAATATAGCAGAAA+TGG + Chr5:34332461-34332480 None:intergenic 20.0%
!! ACCAATAAGTTGTTAAAATT+AGG + Chr5:34332867-34332886 None:intergenic 20.0%
!!! ACCTAATTTTAACAACTTAT+TGG - Chr5:34332863-34332882 MsG0580026299.01.T01:CDS 20.0%
! ATTGCAGAATAAAAGGTAAA+CGG + Chr5:34331855-34331874 None:intergenic 25.0%
! CAAAGTAATGAAACTTTCTT+CGG + Chr5:34332531-34332550 None:intergenic 25.0%
! TATCAAATTAATAGTGGTGT+TGG + Chr5:34332044-34332063 None:intergenic 25.0%
! TGAAACAATTGCAGAATAAA+AGG + Chr5:34331862-34331881 None:intergenic 25.0%
! TTAAATGCTTCTTCATGTAT+AGG - Chr5:34332886-34332905 MsG0580026299.01.T01:CDS 25.0%
! TTAAATTCATAAGCAAGACT+GGG - Chr5:34332707-34332726 MsG0580026299.01.T01:intron 25.0%
! TTTAAATTCATAAGCAAGAC+TGG - Chr5:34332706-34332725 MsG0580026299.01.T01:intron 25.0%
!! ACACATTTCTTTGCTTATTT+TGG + Chr5:34332222-34332241 None:intergenic 25.0%
!! ATATTCCTTTTTCCGATTAT+TGG - Chr5:34332730-34332749 MsG0580026299.01.T01:intron 25.0%
!!! TCAATTTGTTGTAACAGAAA+CGG + Chr5:34332303-34332322 None:intergenic 25.0%
AAATTCGAAGCAAAACTTAG+GGG - Chr5:34332412-34332431 MsG0580026299.01.T01:CDS 30.0%
AAGAATCACTTACCGAAAAA+AGG + Chr5:34331981-34332000 None:intergenic 30.0%
AGATAGATTTCACATAAGCA+CGG + Chr5:34332149-34332168 None:intergenic 30.0%
ATAAGCAAAGAAATGTGTTG+AGG - Chr5:34332224-34332243 MsG0580026299.01.T01:CDS 30.0%
ATATATACTCTTCTTACGCT+TGG - Chr5:34332676-34332695 MsG0580026299.01.T01:intron 30.0%
ATCTAGAAAACTCTGATGAT+TGG + Chr5:34332656-34332675 None:intergenic 30.0%
ATTCTTCAATCATTGAACCA+TGG - Chr5:34331995-34332014 MsG0580026299.01.T01:intron 30.0%
CAAAATTGAATATCAAGGCT+TGG - Chr5:34332591-34332610 MsG0580026299.01.T01:intron 30.0%
CAAATTCGAAGCAAAACTTA+GGG - Chr5:34332411-34332430 MsG0580026299.01.T01:CDS 30.0%
CGTACTAAAGATGGAAAATT+AGG - Chr5:34332977-34332996 MsG0580026299.01.T01:five_prime_UTR 30.0%
TTGCAGAATAAAAGGTAAAC+GGG + Chr5:34331854-34331873 None:intergenic 30.0%
! CTAAGTTTTGCTTCGAATTT+GGG + Chr5:34332412-34332431 None:intergenic 30.0%
! TGCAATTGTTTCATTTTCCA+TGG - Chr5:34331869-34331888 MsG0580026299.01.T01:CDS 30.0%
!! ATTTGGGTTCAAGTATTGAA+TGG + Chr5:34332396-34332415 None:intergenic 30.0%
!! CAATTTGTTGTAACAGAAAC+GGG + Chr5:34332302-34332321 None:intergenic 30.0%
!! TTTGGGTTCAAGTATTGAAT+GGG + Chr5:34332395-34332414 None:intergenic 30.0%
AACATTGCCCAACCAATAAT+CGG + Chr5:34332745-34332764 None:intergenic 35.0%
AATCACTTACCGAAAAAAGG+AGG + Chr5:34331978-34331997 None:intergenic 35.0%
ATCTTACAATGACATCTCTG+CGG + Chr5:34332336-34332355 None:intergenic 35.0%
CCAAATTCGAAGCAAAACTT+AGG - Chr5:34332410-34332429 MsG0580026299.01.T01:CDS 35.0%
CTACTAACATACTTACCATC+TGG + Chr5:34332810-34332829 None:intergenic 35.0%
CTGATGATTGGAAACATAGA+CGG + Chr5:34332644-34332663 None:intergenic 35.0%
CTTAATTGCCTCTTCTTCTA+TGG + Chr5:34331797-34331816 None:intergenic 35.0%
TAAAAGGTAAACGGGAAAGA+AGG + Chr5:34331846-34331865 None:intergenic 35.0%
TGGAAAATTAGGTGCATTCA+AGG - Chr5:34332988-34333007 MsG0580026299.01.T01:five_prime_UTR 35.0%
! CCTAAGTTTTGCTTCGAATT+TGG + Chr5:34332413-34332432 None:intergenic 35.0%
! CTTTTTATCTTCATGCTGCT+TGG - Chr5:34332475-34332494 MsG0580026299.01.T01:CDS 35.0%
! GGTTAGTTGGATTGTATCTA+TGG + Chr5:34331825-34331844 None:intergenic 35.0%
! TCCTTTTTCCGATTATTGGT+TGG - Chr5:34332734-34332753 MsG0580026299.01.T01:intron 35.0%
! TGCATCGATCTGATCAAAAA+AGG - Chr5:34332923-34332942 MsG0580026299.01.T01:exon 35.0%
!! CCTTTTTCCGATTATTGGTT+GGG - Chr5:34332735-34332754 MsG0580026299.01.T01:intron 35.0%
AAACGGGAAAGAAGGTTAGT+TGG + Chr5:34331838-34331857 None:intergenic 40.0%
ACTCATACACGCTTAATGTG+AGG - Chr5:34332118-34332137 MsG0580026299.01.T01:CDS 40.0%
AGAGATAGAGACAACGATGA+TGG + Chr5:34331911-34331930 None:intergenic 40.0%
ATTACTTTGTCGCCAAACTC+TGG - Chr5:34332542-34332561 MsG0580026299.01.T01:CDS 40.0%
CAACGATTCCATAGAAGAAG+AGG - Chr5:34331786-34331805 MsG0580026299.01.T01:CDS 40.0%
CCACACAAGTCGTTGAATTT+GGG + Chr5:34332262-34332281 None:intergenic 40.0%
CCCAACCAATAATCGGAAAA+AGG + Chr5:34332738-34332757 None:intergenic 40.0%
GTAATCCAATTCTGGGTTGT+TGG + Chr5:34331944-34331963 None:intergenic 40.0%
GTCGTTGAATTTGGGAGTAA+AGG + Chr5:34332254-34332273 None:intergenic 40.0%
GTTCCACGTAATCCAATTCT+GGG + Chr5:34331951-34331970 None:intergenic 40.0%
TATGAGATTACTGCTGCAAC+AGG - Chr5:34332355-34332374 MsG0580026299.01.T01:CDS 40.0%
TCCACACAAGTCGTTGAATT+TGG + Chr5:34332263-34332282 None:intergenic 40.0%
TGTTCCACGTAATCCAATTC+TGG + Chr5:34331952-34331971 None:intergenic 40.0%
TTACTTTGTCGCCAAACTCT+GGG - Chr5:34332543-34332562 MsG0580026299.01.T01:CDS 40.0%
TTCTTCGGTAACGATCAACA+AGG + Chr5:34332516-34332535 None:intergenic 40.0%
TTGAACCATGGATACAGACA+CGG - Chr5:34332007-34332026 MsG0580026299.01.T01:CDS 40.0%
! GGTTGGGCAATGTTTCTTTT+GGG - Chr5:34332751-34332770 MsG0580026299.01.T01:intron 40.0%
! TGGTTGGGCAATGTTTCTTT+TGG - Chr5:34332750-34332769 MsG0580026299.01.T01:intron 40.0%
!! TAGAGACAACGATGATGGAT+TGG + Chr5:34331906-34331925 None:intergenic 40.0%
!! TGTAATTCCTTTGAAGCGCA+TGG - Chr5:34332774-34332793 MsG0580026299.01.T01:intron 40.0%
ATTACTGCTGCAACAGGAGA+CGG - Chr5:34332361-34332380 MsG0580026299.01.T01:CDS 45.0%
CAACCCAGAATTGGATTACG+TGG - Chr5:34331945-34331964 MsG0580026299.01.T01:intron 45.0%
CACTTACCGAAAAAAGGAGG+AGG + Chr5:34331975-34331994 None:intergenic 45.0%
CCCAAATTCAACGACTTGTG+TGG - Chr5:34332259-34332278 MsG0580026299.01.T01:CDS 45.0%
GACTTGTGTGGAACTGGAAT+TGG - Chr5:34332271-34332290 MsG0580026299.01.T01:CDS 45.0%
GAGGGAACACGTACTAAAGA+TGG - Chr5:34332968-34332987 MsG0580026299.01.T01:five_prime_UTR 45.0%
GATTGGAGAAGCAACTTCCA+TGG + Chr5:34331889-34331908 None:intergenic 45.0%
TCTTTAGTACGTGTTCCCTC+CGG + Chr5:34332968-34332987 None:intergenic 45.0%
TGATTGGAAACATAGACGGC+TGG + Chr5:34332640-34332659 None:intergenic 45.0%
TTCAACGACTTGTGTGGAAC+TGG - Chr5:34332265-34332284 MsG0580026299.01.T01:CDS 45.0%
! AGCCTCTGTCTTTTTCTTCC+CGG - Chr5:34332946-34332965 MsG0580026299.01.T01:five_prime_UTR 45.0%
! CGATCAACAAGGAGCACAAA+TGG + Chr5:34332505-34332524 None:intergenic 45.0%
! TCTGTCTTTTTCTTCCCGGA+GGG - Chr5:34332950-34332969 MsG0580026299.01.T01:five_prime_UTR 45.0%
!!! GGAACACCTCCTCCTTTTTT+CGG - Chr5:34331966-34331985 MsG0580026299.01.T01:intron 45.0%
AGTGAGTGTAGCCCAGAGTT+TGG + Chr5:34332557-34332576 None:intergenic 50.0%
AGTGTCCGTGTCTGTATCCA+TGG + Chr5:34332015-34332034 None:intergenic 50.0%
CTCCGGGAAGAAAAAGACAG+AGG + Chr5:34332951-34332970 None:intergenic 50.0%
CTTTAGTACGTGTTCCCTCC+GGG + Chr5:34332967-34332986 None:intergenic 50.0%
TCTCGCCAACAACCCAGAAT+TGG - Chr5:34331936-34331955 MsG0580026299.01.T01:intron 50.0%
! ACATAGACGGCTGGAGTATC+TGG + Chr5:34332631-34332650 None:intergenic 50.0%
! CTCTGTCTTTTTCTTCCCGG+AGG - Chr5:34332949-34332968 MsG0580026299.01.T01:five_prime_UTR 50.0%
CATGGACAGTCGAAGCCAGA+TGG - Chr5:34332792-34332811 MsG0580026299.01.T01:intron 55.0%
CGACTGTCCATGCGCTTCAA+AGG + Chr5:34332784-34332803 None:intergenic 55.0%
! ACGGCTGGAGTATCTGGAGA+TGG + Chr5:34332625-34332644 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 34331767 34333048 34331767 ID=MsG0580026299.01;Name=MsG0580026299.01
Chr5 mRNA 34331767 34333048 34331767 ID=MsG0580026299.01.T01;Parent=MsG0580026299.01;Name=MsG0580026299.01.T01;_AED=0.48;_eAED=0.50;_QI=121|1|0.66|1|1|1|3|0|281
Chr5 exon 34331767 34331907 34331767 ID=MsG0580026299.01.T01:exon:25455;Parent=MsG0580026299.01.T01
Chr5 exon 34332002 34332606 34332002 ID=MsG0580026299.01.T01:exon:25454;Parent=MsG0580026299.01.T01
Chr5 exon 34332828 34333048 34332828 ID=MsG0580026299.01.T01:exon:25453;Parent=MsG0580026299.01.T01
Chr5 five_prime_UTR 34332928 34333048 34332928 ID=MsG0580026299.01.T01:five_prime_utr;Parent=MsG0580026299.01.T01
Chr5 CDS 34332828 34332927 34332828 ID=MsG0580026299.01.T01:cds;Parent=MsG0580026299.01.T01
Chr5 CDS 34332002 34332606 34332002 ID=MsG0580026299.01.T01:cds;Parent=MsG0580026299.01.T01
Chr5 CDS 34331767 34331907 34331767 ID=MsG0580026299.01.T01:cds;Parent=MsG0580026299.01.T01
Gene Sequence

>MsG0580026299.01.T01

ATGGAAGTTGCTTCTCCAATCCATCATCGTTGTCTCTATCTCTCTTACTCTCGCCAACAACCCAGAATTGGATTACGTGGAACACCTCCTCCTTTTTTCGTTTCTCAAATCCAAAATAAGCAAAGAAATGTGTTGAGGCTGTCCTTTACTCCCAAATTCAACGACTTGTGTGGAACTGGAATTGGAACAACCCGTTTCTGTTACAACAAATTGAAACAACAACTCCGCAGAGATGTCATTGTAAGATATGAGATTACTGCTGCAACAGGAGACGGAAACAACGCCCATTCAATACTTGAACCCAAATTCGAAGCAAAACTTAGGGGAATTTGTTTTTATACAATTACTTCCATTTCTGCTATATTTCTTTTTATCTTCATGCTGCTTGGACATCCATTTGTGCTCCTTGTTGATCGTTACCGAAGAAAGTTTCATTACTTTGTCGCCAAACTCTGGGCTACACTCACTGTCACTCCATTTTTCAAAATTGAATATCAAGGCTTGGAGAATCTACCATCTCCAGATACTCCAGCCGTCTATGTTTCCAATCATCAGAGTTTTCTAGATATATATACTCTTCTTACGCTTGGAAGAAGCTTTAAATTCATAAGCAAGACTGGGATATTCCTTTTTCCGATTATTGGTTGGGCAATGTTTCTTTTGGGTGTAATTCCTTTGAAGCGCATGGACAGTCGAAGCCAGATGGACTGTCTTAAACGATGCATCGATCTGATCAAAAAAGGAGCCTCTGTCTTTTTCTTCCCGGAGGGAACACGTACTAAAGATGGAAAATTAGGTGCATTCAAGGTTAGTTTTTGTGCAATCACTTTCACCTTTGAAGAGTAG

Protein sequence

>MsG0580026299.01.T01

MEVASPIHHRCLYLSYSRQQPRIGLRGTPPPFFVSQIQNKQRNVLRLSFTPKFNDLCGTGIGTTRFCYNKLKQQLRRDVIVRYEITAATGDGNNAHSILEPKFEAKLRGICFYTITSISAIFLFIFMLLGHPFVLLVDRYRRKFHYFVAKLWATLTVTPFFKIEYQGLENLPSPDTPAVYVSNHQSFLDIYTLLTLGRSFKFISKTGIFLFPIIGWAMFLLGVIPLKRMDSRSQMDCLKRCIDLIKKGASVFFFPEGTRTKDGKLGAFKVSFCAITFTFEE*