AlfalfaGEDB | MsG0180002454.01

M. sativa cultivar ZhongmuNo.1 / MsG0180002454.01

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002454.01.T01	XP_003590259.1	83.051	118	12	2	1	111	3	119	2.68E-52	174
MsG0180002454.01.T02	XP_003590259.1	87.805	123	11	3	1	123	1	119	2.78E-58	189

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002454.01.T01	G7I7V4	83.051	118	12	2	1	111	3	119	1.28e-52	174
MsG0180002454.01.T02	G7I7V4	87.805	123	11	3	1	123	1	119	1.33e-58	189

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047999.01	MsG0180002454.01	-0.801051	1.080346e-48	5.109585e-46
MsG0080048059.01	MsG0180002454.01	0.852539	4.320241e-61	9.056292e-58
MsG0080048202.01	MsG0180002454.01	-0.819837	9.415555e-53	7.326998e-50
MsG0080048694.01	MsG0180002454.01	0.804239	2.376430e-49	1.218995e-46
MsG0080048695.01	MsG0180002454.01	0.804647	1.953736e-49	1.012816e-46
MsG0080048800.01	MsG0180002454.01	0.800071	1.711404e-48	7.894939e-46
MsG0080048887.01	MsG0180002454.01	0.825869	3.704337e-54	3.418408e-51
MsG0080048896.01	MsG0180002454.01	0.838322	3.080652e-57	4.111218e-54
MsG0080048918.01	MsG0180002454.01	0.816793	4.604183e-52	3.294231e-49
MsG0080048919.01	MsG0180002454.01	0.834999	2.166256e-56	2.614320e-53

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002454.01.T01	MTR_1g050700	83.051	118	12	2	1	111	3	119	3.24e-56	174
MsG0180002454.01.T02	MTR_1g050700	87.805	123	11	3	1	123	1	119	3.37e-62	189

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002454.01.T01	AT4G40045	34.426	122	64	5	1	112	1	116	1.46e-11	59.7
MsG0180002454.01.T02	AT4G40045	34.959	123	72	4	3	124	1	116	1.92e-14	67.4

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Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAACAACAATAACAGTAA+TGG	0.472073	1:-38593782	MsG0180002454.01.T01:CDS
ACAACAACAATAACAGTAAT+GGG	0.303617	1:-38593781	MsG0180002454.01.T01:CDS
AGAATTGAAGGTGATGGAAA+TGG	0.474902	1:+38593838	None:intergenic
AGCAATAAAAGAGTATTTGA+GGG	0.510084	1:+38593536	None:intergenic
AGTAGCGATATGAGCATGTT+AGG	0.301511	1:-38593932	None:intergenic
ATCCTTGCTCTTGGATTAAC+TGG	0.244904	1:-38593629	MsG0180002454.01.T01:CDS
ATTAACTGGTATTCTCACAT+GGG	0.657152	1:-38593615	MsG0180002454.01.T01:CDS
ATTACTGTTATTGTTGTTGT+TGG	0.325641	1:+38593784	None:intergenic
CAACAACAATAACAGTAATG+GGG	0.675070	1:-38593780	MsG0180002454.01.T01:CDS
CAGCAATAAAAGAGTATTTG+AGG	0.478257	1:+38593535	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCCAATTCAAACCAAAAA+GGG	+	Chr1:38593617-38593636	None:intergenic	30.0%
ATTAACTGGTATTCTCACAT+GGG	-	Chr1:38593678-38593697	MsG0180002454.01.T01:CDS	30.0%
CAACAACAATAACAGTAATG+GGG	-	Chr1:38593513-38593532	MsG0180002454.01.T01:CDS	30.0%
TACTCCAATTCAAACCAAAA+AGG	+	Chr1:38593618-38593637	None:intergenic	30.0%
CTCCAATTCAAACCAAAAAG+GGG	+	Chr1:38593616-38593635	None:intergenic	35.0%
GATTAACTGGTATTCTCACA+TGG	-	Chr1:38593677-38593696	MsG0180002454.01.T01:CDS	35.0%
TATCAGGATCTCGATCTAAA+AGG	+	Chr1:38593642-38593661	None:intergenic	35.0%
ATCCTTGCTCTTGGATTAAC+TGG	-	Chr1:38593664-38593683	MsG0180002454.01.T01:CDS	40.0%
CCAAGAGCAAGGATATTATC+AGG	+	Chr1:38593658-38593677	None:intergenic	40.0%
CCTGATAATATCCTTGCTCT+TGG	-	Chr1:38593655-38593674	MsG0180002454.01.T01:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	38593370	38593945	38593370	ID=MsG0180002454.01;Name=MsG0180002454.01
Chr1	mRNA	38593370	38593939	38593370	ID=MsG0180002454.01.T01;Parent=MsG0180002454.01;Name=MsG0180002454.01.T01;_AED=0.47;_eAED=0.60;_QI=0\|0\|0\|1\|0\|0\|2\|0\|179
Chr1	exon	38593753	38593939	38593753	ID=MsG0180002454.01.T01:exon:12785;Parent=MsG0180002454.01.T01
Chr1	exon	38593370	38593722	38593370	ID=MsG0180002454.01.T01:exon:12786;Parent=MsG0180002454.01.T01
Chr1	CDS	38593753	38593939	38593753	ID=MsG0180002454.01.T01:cds;Parent=MsG0180002454.01.T01
Chr1	CDS	38593370	38593722	38593370	ID=MsG0180002454.01.T01:cds;Parent=MsG0180002454.01.T01
Chr1	mRNA	38593370	38593945	38593370	ID=MsG0180002454.01.T02;Parent=MsG0180002454.01;Name=MsG0180002454.01.T02;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|191
Chr1	exon	38593370	38593945	38593370	ID=MsG0180002454.01.T02:exon:12787;Parent=MsG0180002454.01.T02
Chr1	CDS	38593370	38593945	38593370	ID=MsG0180002454.01.T02:cds;Parent=MsG0180002454.01.T02

Gene Sequence

>MsG0180002454.01.T02

ATGAGCATGTTAGGGTTAACCAATTTCATACTCTCATCATCACTGTCAATCCCTTCTCTTCAATACAAACCACAACACTTTTCTACCATTTCCATCACCTTCAATTCTCGAAAACCACGCCCACTTCCATTGTCTGCATCCAACAACAACAATAACAGTAATGGGGTAAAAGAAGAGGACAAAAATTCACAACAACAGCCACCACCCTCTTCTTCTGGACAAGAACAAAAACAAGAAAAACTACCCCTTTTTGGTTTGAATTGGAGTAGCCTTTTAGATCGAGATCCTGATAATATCCTTGCTCTTGGATTAACTGGTATTCTCACATGGGCAAGTGTTCAAGTTCTGTGGCAACTCTTGTTTATCTCTTGGCTATTCTCGTTGCTGCCCTCAAATACTCTTTTATTGCTGCTCTTCTCATTTTCATTCTCATCGCTCTTCTCTAATTCATTACATTTTCTTGCTCACTTGTTTGTAATGTCATGTTCATATATATCTAATGGAAAGAAAATTCATTACATAATTATTTTCCGTTTATATTATGTTAAAGCTATTGTGCATAAGACCTCAAAATGA

>MsG0180002454.01.T01

ATGTTAGGGTTAACCAATTTCATACTCTCATCATCACTGTCAATCCCTTCTCTTCAATACAAACCACAACACTTTTCTACCATTTCCATCACCTTCAATTCTCGAAAACCACGCCCACTTCCATTGTCTGCATCCAACAACAACAATAACAGTAATGGGGTAAAAGAAGAGGACAAAAATTCACAACAACAAAAACAAGAAAAACTACCCCTTTTTGGTTTGAATTGGAGTAGCCTTTTAGATCGAGATCCTGATAATATCCTTGCTCTTGGATTAACTGGTATTCTCACATGGGCAAGTGTTCAAGTTCTGTGGCAACTCTTGTTTATCTCTTGGCTATTCTCGTTGCTGCCCTCAAATACTCTTTTATTGCTGCTCTTCTCATTTTCATTCTCATCGCTCTTCTCTAATTCATTACATTTTCTTGCTCACTTGTTTGTAATGTCATGTTCATATATATCTAATGGAAAGAAAATTCATTACATAATTATTTTCCGTTTATATTATGTTAAAGCTATTGTGCATAAGACCTCAAAATGA

Protein sequence

>MsG0180002454.01.T02

MSMLGLTNFILSSSLSIPSLQYKPQHFSTISITFNSRKPRPLPLSASNNNNNSNGVKEEDKNSQQQPPPSSSGQEQKQEKLPLFGLNWSSLLDRDPDNILALGLTGILTWASVQVLWQLLFISWLFSLLPSNTLLLLLFSFSFSSLFSNSLHFLAHLFVMSCSYISNGKKIHYIIIFRLYYVKAIVHKTSK*

>MsG0180002454.01.T01

MLGLTNFILSSSLSIPSLQYKPQHFSTISITFNSRKPRPLPLSASNNNNNSNGVKEEDKNSQQQKQEKLPLFGLNWSSLLDRDPDNILALGLTGILTWASVQVLWQLLFISWLFSLLPSNTLLLLLFSFSFSSLFSNSLHFLAHLFVMSCSYISNGKKIHYIIIFRLYYVKAIVHKTSK*