AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0237230


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0237230 MtrunA17_Chr2g0286581 83.688 141 10 3 1 139 68 197 2.86e-63 192
Msa0237230 MtrunA17_Chr2g0287391 45.522 134 58 3 1 132 59 179 5.11e-19 78.6
Msa0237230 MtrunA17_Chr2g0287341 52.308 65 31 0 75 139 136 200 1.74e-15 69.7
Msa0237230 MtrunA17_Chr2g0287401 55.357 56 23 1 84 139 113 166 2.33e-12 60.8
Msa0237230 MtrunA17_Chr2g0287311 39.865 148 75 4 1 135 68 214 1.22e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 22 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTAAGTTGGATGGTGTTT+TGG 0.344627 2_2:+10313392 Msa0237230:CDS
TCTTGTTCTTGAGGCACGAC+CGG 0.413059 2_2:-10313213 None:intergenic
CAGCTTGTATTTCCGTATCA+AGG 0.431227 2_2:-10313455 None:intergenic
AATGACTGCACTTGCTAAGT+TGG 0.443221 2_2:+10313380 Msa0237230:CDS
GATAAATTCGCATAAAGCTT+CGG 0.452560 2_2:-10313500 None:intergenic
CTTGTTCTTGAGGCACGACC+GGG 0.453782 2_2:-10313212 None:intergenic
CAAATTTATCAACCTTGATA+CGG 0.464878 2_2:+10313443 Msa0237230:CDS
GACAATGCTGCTGGCGAAGA+AGG 0.488462 2_2:+10313282 Msa0237230:CDS
ACCACTTGTTCTTGTTCTTG+AGG 0.506510 2_2:-10313222 None:intergenic
TAAATGTTCAGTCTCTCAAA+GGG 0.514280 2_2:+10313415 Msa0237230:CDS
GTAAATGTTCAGTCTCTCAA+AGG 0.517114 2_2:+10313414 Msa0237230:CDS
CAAGAAGATGACAATGCTGC+TGG 0.553932 2_2:+10313273 Msa0237230:CDS
AAGAAGAAGATGAGAAACAT+TGG 0.556347 2_2:+10313355 Msa0237230:CDS
GAACAAGTGGTTGATGAACA+TGG 0.574045 2_2:+10313234 Msa0237230:CDS
GCCTCAAGAACAAGAACAAG+TGG 0.580856 2_2:+10313221 Msa0237230:CDS
GAATAATCAGAATTAAGATG+AGG 0.601526 2_2:-10313541 None:intergenic
TGATACGGAAATACAAGCTG+TGG 0.623711 2_2:+10313458 Msa0237230:CDS
TACGGAAATACAAGCTGTGG+AGG 0.638102 2_2:+10313461 Msa0237230:CDS
ACTGCACTTGCTAAGTTGGA+TGG 0.644780 2_2:+10313384 Msa0237230:CDS
GAAAGCATTGAACTGTTCCG+CGG 0.670247 2_2:-10313166 None:intergenic
ACAGCATCTTATAACTAACC+CGG 0.698038 2_2:+10313194 Msa0237230:CDS
AAATGTTCAGTCTCTCAAAG+GGG 0.709545 2_2:+10313416 Msa0237230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAAATTTATCAACCTTGATA+CGG + chr2_2:10313443-10313462 Msa0237230:CDS 25.0%
AAGAAGAAGATGAGAAACAT+TGG + chr2_2:10313355-10313374 Msa0237230:CDS 30.0%
TAAATGTTCAGTCTCTCAAA+GGG + chr2_2:10313415-10313434 Msa0237230:CDS 30.0%
GATAAATTCGCATAAAGCTT+CGG - chr2_2:10313503-10313522 None:intergenic 30.0%
ACAGCATCTTATAACTAACC+CGG + chr2_2:10313194-10313213 Msa0237230:CDS 35.0%
GTAAATGTTCAGTCTCTCAA+AGG + chr2_2:10313414-10313433 Msa0237230:CDS 35.0%
AAATGTTCAGTCTCTCAAAG+GGG + chr2_2:10313416-10313435 Msa0237230:CDS 35.0%
CGCGGTCTTAGAAAAAAGAA+CGG - chr2_2:10313151-10313170 None:intergenic 40.0%
!!! CGTTCTTTTTTCTAAGACCG+CGG + chr2_2:10313149-10313168 Msa0237230:CDS 40.0%
ACCACTTGTTCTTGTTCTTG+AGG - chr2_2:10313225-10313244 None:intergenic 40.0%
!! GAACAAGTGGTTGATGAACA+TGG + chr2_2:10313234-10313253 Msa0237230:CDS 40.0%
! AATGACTGCACTTGCTAAGT+TGG + chr2_2:10313380-10313399 Msa0237230:CDS 40.0%
!! TGCTAAGTTGGATGGTGTTT+TGG + chr2_2:10313392-10313411 Msa0237230:CDS 40.0%
CAGCTTGTATTTCCGTATCA+AGG - chr2_2:10313458-10313477 None:intergenic 40.0%
TGATACGGAAATACAAGCTG+TGG + chr2_2:10313458-10313477 Msa0237230:CDS 40.0%
!! GAAAGCATTGAACTGTTCCG+CGG - chr2_2:10313169-10313188 None:intergenic 45.0%
GCCTCAAGAACAAGAACAAG+TGG + chr2_2:10313221-10313240 Msa0237230:CDS 45.0%
CAAGAAGATGACAATGCTGC+TGG + chr2_2:10313273-10313292 Msa0237230:CDS 45.0%
! ACTGCACTTGCTAAGTTGGA+TGG + chr2_2:10313384-10313403 Msa0237230:CDS 45.0%
TACGGAAATACAAGCTGTGG+AGG + chr2_2:10313461-10313480 Msa0237230:CDS 45.0%
TCTTGTTCTTGAGGCACGAC+CGG - chr2_2:10313216-10313235 None:intergenic 50.0%
CTTGTTCTTGAGGCACGACC+GGG - chr2_2:10313215-10313234 None:intergenic 55.0%
GACAATGCTGCTGGCGAAGA+AGG + chr2_2:10313282-10313301 Msa0237230:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 10313132 10313551 10313132 ID=Msa0237230;Name=Msa0237230
chr2_2 mRNA 10313132 10313551 10313132 ID=Msa0237230-mRNA-1;Parent=Msa0237230;Name=Msa0237230-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|-1|0|1|-1|1|1|0|139
chr2_2 exon 10313132 10313551 10313132 ID=Msa0237230-mRNA-1:exon:4434;Parent=Msa0237230-mRNA-1
chr2_2 CDS 10313132 10313551 10313132 ID=Msa0237230-mRNA-1:cds;Parent=Msa0237230-mRNA-1
Gene Sequence

>Msa0237230

TTGCTCAATGATATCACCGTTCTTTTTTCTAAGACCGCGGAACAGTTCAATGCTTTCAAATCACAGCATCTTATAACTAACCCGGTCGTGCCTCAAGAACAAGAACAAGTGGTTGATGAACATGGCGAAGAAGAAGCAGCACAAGAAGATGACAATGCTGCTGGCGAAGAAGGTGATGCTGCTGATGAAGAAGAAGAAGAAGAAGAGAATGTTGTTGATGCTGAAGAAGAAGATGAGAAACATTGGTTAATGACTGCACTTGCTAAGTTGGATGGTGTTTTGGTAAATGTTCAGTCTCTCAAAGGGGATTTCAAATTTATCAACCTTGATACGGAAATACAAGCTGTGGAGGATAGAATGAAGTGTCTCCGAAGCTTTATGCGAATTTATCTCAAGTCAGAAACATTTTCCTCATCTTAA

Protein sequence

>Msa0237230

LLNDITVLFSKTAEQFNAFKSQHLITNPVVPQEQEQVVDEHGEEEAAQEDDNAAGEEGDAADEEEEEEENVVDAEEEDEKHWLMTALAKLDGVLVNVQSLKGDFKFINLDTEIQAVEDRMKCLRSFMRIYLKSETFSSS*