AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0182680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTCTCTCATGGCTGCTTT+AGG 0.161971 1_4:+71469770 None:intergenic
GGTCTCTCATGGCTGCTTTA+GGG 0.214452 1_4:+71469771 None:intergenic
ACTGCTGCTTCCTTGGGTTC+TGG 0.294667 1_4:-71469851 None:intergenic
TTAGGGCCTCTATATGGATT+TGG 0.301039 1_4:+71469788 Msa0182680:CDS
ACTTGCTACTGCTGCTTCCT+TGG 0.301701 1_4:-71469858 None:intergenic
GTTGATTGGAAGAAGACATT+TGG 0.356196 1_4:+71471053 Msa0182680:CDS
CTTGCTACTGCTGCTTCCTT+GGG 0.363903 1_4:-71469857 None:intergenic
TTCTTATTAGAGTCTGCTTT+GGG 0.365219 1_4:+71469821 Msa0182680:CDS
TCAACGTGACTTGTTGAAAA+TGG 0.365372 1_4:-71471035 None:intergenic
TTTCAACAAGTCACGTTGAT+TGG 0.367669 1_4:+71471039 Msa0182680:CDS
GCTGCTTTAGGGCCTCTATA+TGG 0.374017 1_4:+71469782 Msa0182680:CDS
TTTCTTATTAGAGTCTGCTT+TGG 0.405671 1_4:+71469820 Msa0182680:CDS
TTGATTGGAAGAAGACATTT+GGG 0.436458 1_4:+71471054 Msa0182680:CDS
CAGACTCTAATAAGAAAGTC+TGG 0.448790 1_4:-71469814 None:intergenic
TGGTAACCAAATCCATATAG+AGG 0.522436 1_4:-71469794 None:intergenic
TTTCTTATGCAAATAAACAA+TGG 0.564955 1_4:-71471171 None:intergenic
TTCAGACATAACAAATGCAG+AGG 0.605231 1_4:+71471004 Msa0182680:CDS
GGGAACAAAACCAGAACCCA+AGG 0.621328 1_4:+71469841 Msa0182680:CDS
TTATTGCTAGAACAAAGTAG+TGG 0.679896 1_4:-71471205 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAATTAATTTCAACA+TGG - chr1_4:71470934-71470953 None:intergenic 10.0%
!! AATAAAAATTAATTTCAACA+TGG - chr1_4:71470934-71470953 None:intergenic 10.0%
!!! CTAATAGACTTTTAGATTAT+TGG - chr1_4:71470065-71470084 None:intergenic 20.0%
!! TTGGAATATAGTTTCTTAAA+TGG - chr1_4:71470121-71470140 None:intergenic 20.0%
!!! TCATATTTTTGCCTTTTATT+TGG + chr1_4:71470429-71470448 Msa0182680:intron 20.0%
!! TTTCTTATGCAAATAAACAA+TGG - chr1_4:71471174-71471193 None:intergenic 20.0%
!! TAGCAGTATCAAAAAATTAA+TGG - chr1_4:71471237-71471256 None:intergenic 20.0%
!!! CTAATAGACTTTTAGATTAT+TGG - chr1_4:71470065-71470084 None:intergenic 20.0%
!! TTGGAATATAGTTTCTTAAA+TGG - chr1_4:71470121-71470140 None:intergenic 20.0%
!!! TCATATTTTTGCCTTTTATT+TGG + chr1_4:71470429-71470448 Msa0182680:intron 20.0%
!! TTTCTTATGCAAATAAACAA+TGG - chr1_4:71471174-71471193 None:intergenic 20.0%
!! TAGCAGTATCAAAAAATTAA+TGG - chr1_4:71471237-71471256 None:intergenic 20.0%
!! ATATATGTGAGTGACTATTT+TGG + chr1_4:71470038-71470057 Msa0182680:intron 25.0%
! TTTCTACATTATGCATACTA+TGG - chr1_4:71470089-71470108 None:intergenic 25.0%
! GAACAAGTATGTGTTTATAA+AGG + chr1_4:71470193-71470212 Msa0182680:intron 25.0%
! AGAAAAAATCGCCAAATAAA+AGG - chr1_4:71470443-71470462 None:intergenic 25.0%
! AGTTATGGGTATAAAAAAGA+AGG + chr1_4:71470604-71470623 Msa0182680:intron 25.0%
! TCTACCTGTATAATTTGAAT+TGG - chr1_4:71470743-71470762 None:intergenic 25.0%
!! ATATATGTGAGTGACTATTT+TGG + chr1_4:71470038-71470057 Msa0182680:intron 25.0%
! TTTCTACATTATGCATACTA+TGG - chr1_4:71470089-71470108 None:intergenic 25.0%
! GAACAAGTATGTGTTTATAA+AGG + chr1_4:71470193-71470212 Msa0182680:intron 25.0%
! AGAAAAAATCGCCAAATAAA+AGG - chr1_4:71470443-71470462 None:intergenic 25.0%
! AGTTATGGGTATAAAAAAGA+AGG + chr1_4:71470604-71470623 Msa0182680:intron 25.0%
! TCTACCTGTATAATTTGAAT+TGG - chr1_4:71470743-71470762 None:intergenic 25.0%
TTTCTTATTAGAGTCTGCTT+TGG + chr1_4:71469820-71469839 Msa0182680:CDS 30.0%
TTCTTATTAGAGTCTGCTTT+GGG + chr1_4:71469821-71469840 Msa0182680:CDS 30.0%
TATATATACCTGGTTTAGCT+TGG - chr1_4:71469906-71469925 None:intergenic 30.0%
GAAGTGAACATATATATACC+TGG - chr1_4:71469916-71469935 None:intergenic 30.0%
CAAAACCAATGTTAAAGTAG+TGG - chr1_4:71469958-71469977 None:intergenic 30.0%
!!! CACTACTTTAACATTGGTTT+TGG + chr1_4:71469956-71469975 Msa0182680:intron 30.0%
TAGTCACTCACATATATAGA+GGG - chr1_4:71470035-71470054 None:intergenic 30.0%
ATAGTCACTCACATATATAG+AGG - chr1_4:71470036-71470055 None:intergenic 30.0%
AAGATGGTGTAGTATTAAGT+TGG - chr1_4:71470140-71470159 None:intergenic 30.0%
AACTTAATACTACACCATCT+TGG + chr1_4:71470139-71470158 Msa0182680:intron 30.0%
AGTATGTGTTTATAAAGGTC+TGG + chr1_4:71470198-71470217 Msa0182680:intron 30.0%
!!! CTCTGTTTTGATGTTGTTAT+TGG + chr1_4:71470461-71470480 Msa0182680:intron 30.0%
ATATGAGATTTCAACATGTG+TGG - chr1_4:71470493-71470512 None:intergenic 30.0%
! TTCAATGCATCTGAAGTTAT+GGG + chr1_4:71470590-71470609 Msa0182680:intron 30.0%
ATGGGTATAAAAAAGAAGGA+TGG + chr1_4:71470608-71470627 Msa0182680:intron 30.0%
ATAATTTGAATTGGCATGGT+GGG - chr1_4:71470734-71470753 None:intergenic 30.0%
TATAATTTGAATTGGCATGG+TGG - chr1_4:71470735-71470754 None:intergenic 30.0%
CTGTATAATTTGAATTGGCA+TGG - chr1_4:71470738-71470757 None:intergenic 30.0%
CATGCCAATTCAAATTATAC+AGG + chr1_4:71470736-71470755 Msa0182680:intron 30.0%
! CAGAATGTATTGTTTTCACA+TGG + chr1_4:71470794-71470813 Msa0182680:intron 30.0%
TTGATTGGAAGAAGACATTT+GGG + chr1_4:71471054-71471073 Msa0182680:CDS 30.0%
TTATTGCTAGAACAAAGTAG+TGG - chr1_4:71471208-71471227 None:intergenic 30.0%
TTTCTTATTAGAGTCTGCTT+TGG + chr1_4:71469820-71469839 Msa0182680:CDS 30.0%
TTCTTATTAGAGTCTGCTTT+GGG + chr1_4:71469821-71469840 Msa0182680:CDS 30.0%
TATATATACCTGGTTTAGCT+TGG - chr1_4:71469906-71469925 None:intergenic 30.0%
GAAGTGAACATATATATACC+TGG - chr1_4:71469916-71469935 None:intergenic 30.0%
CAAAACCAATGTTAAAGTAG+TGG - chr1_4:71469958-71469977 None:intergenic 30.0%
!!! CACTACTTTAACATTGGTTT+TGG + chr1_4:71469956-71469975 Msa0182680:intron 30.0%
TAGTCACTCACATATATAGA+GGG - chr1_4:71470035-71470054 None:intergenic 30.0%
ATAGTCACTCACATATATAG+AGG - chr1_4:71470036-71470055 None:intergenic 30.0%
AAGATGGTGTAGTATTAAGT+TGG - chr1_4:71470140-71470159 None:intergenic 30.0%
AACTTAATACTACACCATCT+TGG + chr1_4:71470139-71470158 Msa0182680:intron 30.0%
AGTATGTGTTTATAAAGGTC+TGG + chr1_4:71470198-71470217 Msa0182680:intron 30.0%
!!! CTCTGTTTTGATGTTGTTAT+TGG + chr1_4:71470461-71470480 Msa0182680:intron 30.0%
ATATGAGATTTCAACATGTG+TGG - chr1_4:71470493-71470512 None:intergenic 30.0%
! TTCAATGCATCTGAAGTTAT+GGG + chr1_4:71470590-71470609 Msa0182680:intron 30.0%
ATGGGTATAAAAAAGAAGGA+TGG + chr1_4:71470608-71470627 Msa0182680:intron 30.0%
ATAATTTGAATTGGCATGGT+GGG - chr1_4:71470734-71470753 None:intergenic 30.0%
TATAATTTGAATTGGCATGG+TGG - chr1_4:71470735-71470754 None:intergenic 30.0%
CTGTATAATTTGAATTGGCA+TGG - chr1_4:71470738-71470757 None:intergenic 30.0%
CATGCCAATTCAAATTATAC+AGG + chr1_4:71470736-71470755 Msa0182680:intron 30.0%
! CAGAATGTATTGTTTTCACA+TGG + chr1_4:71470794-71470813 Msa0182680:intron 30.0%
TTGATTGGAAGAAGACATTT+GGG + chr1_4:71471054-71471073 Msa0182680:CDS 30.0%
TTATTGCTAGAACAAAGTAG+TGG - chr1_4:71471208-71471227 None:intergenic 30.0%
TGGTAACCAAATCCATATAG+AGG - chr1_4:71469797-71469816 None:intergenic 35.0%
CAGACTCTAATAAGAAAGTC+TGG - chr1_4:71469817-71469836 None:intergenic 35.0%
TTCCATATTCTAGCCAATGT+GGG - chr1_4:71470176-71470195 None:intergenic 35.0%
TGTGAAGAAGCTATGAATTC+TGG + chr1_4:71470258-71470277 Msa0182680:intron 35.0%
GTTAAAACAACGACAGCTTT+AGG - chr1_4:71470301-71470320 None:intergenic 35.0%
GGGTTACATCATTTGTTTGA+CGG - chr1_4:71470539-71470558 None:intergenic 35.0%
AAGCAATTGTTCTGTCATCA+GGG - chr1_4:71470559-71470578 None:intergenic 35.0%
AAAGCAATTGTTCTGTCATC+AGG - chr1_4:71470560-71470579 None:intergenic 35.0%
! CTTCAATGCATCTGAAGTTA+TGG + chr1_4:71470589-71470608 Msa0182680:intron 35.0%
TTTCTCTCTAGACAACTCTT+AGG - chr1_4:71470656-71470675 None:intergenic 35.0%
CAAATTATACAGGTAGAGCA+AGG + chr1_4:71470746-71470765 Msa0182680:intron 35.0%
TGTCAAGATACACTAGCAAT+TGG + chr1_4:71470818-71470837 Msa0182680:intron 35.0%
!!! TCTTTTTTGGATGTGTCAGA+AGG + chr1_4:71470953-71470972 Msa0182680:intron 35.0%
TTCAGACATAACAAATGCAG+AGG + chr1_4:71471004-71471023 Msa0182680:CDS 35.0%
!! TCAACGTGACTTGTTGAAAA+TGG - chr1_4:71471038-71471057 None:intergenic 35.0%
TTTCAACAAGTCACGTTGAT+TGG + chr1_4:71471039-71471058 Msa0182680:CDS 35.0%
GTTGATTGGAAGAAGACATT+TGG + chr1_4:71471053-71471072 Msa0182680:CDS 35.0%
TGGTAACCAAATCCATATAG+AGG - chr1_4:71469797-71469816 None:intergenic 35.0%
CAGACTCTAATAAGAAAGTC+TGG - chr1_4:71469817-71469836 None:intergenic 35.0%
TTCCATATTCTAGCCAATGT+GGG - chr1_4:71470176-71470195 None:intergenic 35.0%
TGTGAAGAAGCTATGAATTC+TGG + chr1_4:71470258-71470277 Msa0182680:intron 35.0%
GTTAAAACAACGACAGCTTT+AGG - chr1_4:71470301-71470320 None:intergenic 35.0%
GGGTTACATCATTTGTTTGA+CGG - chr1_4:71470539-71470558 None:intergenic 35.0%
AAGCAATTGTTCTGTCATCA+GGG - chr1_4:71470559-71470578 None:intergenic 35.0%
AAAGCAATTGTTCTGTCATC+AGG - chr1_4:71470560-71470579 None:intergenic 35.0%
! CTTCAATGCATCTGAAGTTA+TGG + chr1_4:71470589-71470608 Msa0182680:intron 35.0%
TTTCTCTCTAGACAACTCTT+AGG - chr1_4:71470656-71470675 None:intergenic 35.0%
CAAATTATACAGGTAGAGCA+AGG + chr1_4:71470746-71470765 Msa0182680:intron 35.0%
TGTCAAGATACACTAGCAAT+TGG + chr1_4:71470818-71470837 Msa0182680:intron 35.0%
!!! TCTTTTTTGGATGTGTCAGA+AGG + chr1_4:71470953-71470972 Msa0182680:intron 35.0%
TTCAGACATAACAAATGCAG+AGG + chr1_4:71471004-71471023 Msa0182680:CDS 35.0%
!! TCAACGTGACTTGTTGAAAA+TGG - chr1_4:71471038-71471057 None:intergenic 35.0%
TTTCAACAAGTCACGTTGAT+TGG + chr1_4:71471039-71471058 Msa0182680:CDS 35.0%
GTTGATTGGAAGAAGACATT+TGG + chr1_4:71471053-71471072 Msa0182680:CDS 35.0%
TTAGGGCCTCTATATGGATT+TGG + chr1_4:71469788-71469807 Msa0182680:CDS 40.0%
!! GTTTAGCTTGGAGTAACATC+AGG - chr1_4:71469894-71469913 None:intergenic 40.0%
ATGTTACTCCAAGCTAAACC+AGG + chr1_4:71469895-71469914 Msa0182680:intron 40.0%
GCGTTCCACTACTTTAACAT+TGG + chr1_4:71469950-71469969 Msa0182680:intron 40.0%
GTTCCATATTCTAGCCAATG+TGG - chr1_4:71470177-71470196 None:intergenic 40.0%
TTTATAAAGGTCTGGCATGC+TGG + chr1_4:71470206-71470225 Msa0182680:intron 40.0%
AAATGCACGCACAAATTCCA+GGG - chr1_4:71470364-71470383 None:intergenic 40.0%
GATGGTGTATCCAGAAGAAA+AGG + chr1_4:71470626-71470645 Msa0182680:intron 40.0%
! CTTAGGTCTACCTTTTCTTC+TGG - chr1_4:71470639-71470658 None:intergenic 40.0%
GAGCAAGGTAGAACTTATAC+AGG + chr1_4:71470761-71470780 Msa0182680:intron 40.0%
TTAGGGCCTCTATATGGATT+TGG + chr1_4:71469788-71469807 Msa0182680:CDS 40.0%
!! GTTTAGCTTGGAGTAACATC+AGG - chr1_4:71469894-71469913 None:intergenic 40.0%
ATGTTACTCCAAGCTAAACC+AGG + chr1_4:71469895-71469914 Msa0182680:intron 40.0%
GCGTTCCACTACTTTAACAT+TGG + chr1_4:71469950-71469969 Msa0182680:intron 40.0%
GTTCCATATTCTAGCCAATG+TGG - chr1_4:71470177-71470196 None:intergenic 40.0%
TTTATAAAGGTCTGGCATGC+TGG + chr1_4:71470206-71470225 Msa0182680:intron 40.0%
AAATGCACGCACAAATTCCA+GGG - chr1_4:71470364-71470383 None:intergenic 40.0%
GATGGTGTATCCAGAAGAAA+AGG + chr1_4:71470626-71470645 Msa0182680:intron 40.0%
! CTTAGGTCTACCTTTTCTTC+TGG - chr1_4:71470639-71470658 None:intergenic 40.0%
GAGCAAGGTAGAACTTATAC+AGG + chr1_4:71470761-71470780 Msa0182680:intron 40.0%
! GTCCCACATTGGCTAGAATA+TGG + chr1_4:71470171-71470190 Msa0182680:intron 45.0%
TGATCACGTCCTTGCTAACT+TGG + chr1_4:71470334-71470353 Msa0182680:intron 45.0%
GATCACGTCCTTGCTAACTT+GGG + chr1_4:71470335-71470354 Msa0182680:intron 45.0%
CAAATGCACGCACAAATTCC+AGG - chr1_4:71470365-71470384 None:intergenic 45.0%
! GTCCCACATTGGCTAGAATA+TGG + chr1_4:71470171-71470190 Msa0182680:intron 45.0%
TGATCACGTCCTTGCTAACT+TGG + chr1_4:71470334-71470353 Msa0182680:intron 45.0%
GATCACGTCCTTGCTAACTT+GGG + chr1_4:71470335-71470354 Msa0182680:intron 45.0%
CAAATGCACGCACAAATTCC+AGG - chr1_4:71470365-71470384 None:intergenic 45.0%
!!! AATTAATTTTTATTCTTTTT+TGG + chr1_4:71470940-71470959 Msa0182680:intron 5.0%
!!! AATTAATTTTTATTCTTTTT+TGG + chr1_4:71470940-71470959 Msa0182680:intron 5.0%
!! GCTGCTTTAGGGCCTCTATA+TGG + chr1_4:71469782-71469801 Msa0182680:CDS 50.0%
GGGAACAAAACCAGAACCCA+AGG + chr1_4:71469841-71469860 Msa0182680:CDS 50.0%
! CTTGCTACTGCTGCTTCCTT+GGG - chr1_4:71469860-71469879 None:intergenic 50.0%
! ACTTGCTACTGCTGCTTCCT+TGG - chr1_4:71469861-71469880 None:intergenic 50.0%
GGGACTTCATGGATCCAAGA+TGG - chr1_4:71470156-71470175 None:intergenic 50.0%
GGATCCATGAAGTCCCACAT+TGG + chr1_4:71470160-71470179 Msa0182680:intron 50.0%
CTAGCCAATGTGGGACTTCA+TGG - chr1_4:71470167-71470186 None:intergenic 50.0%
CAAGCTAGCATTACCCAGCT+CGG - chr1_4:71470240-71470259 None:intergenic 50.0%
!! GCTGCTTTAGGGCCTCTATA+TGG + chr1_4:71469782-71469801 Msa0182680:CDS 50.0%
GGGAACAAAACCAGAACCCA+AGG + chr1_4:71469841-71469860 Msa0182680:CDS 50.0%
! CTTGCTACTGCTGCTTCCTT+GGG - chr1_4:71469860-71469879 None:intergenic 50.0%
! ACTTGCTACTGCTGCTTCCT+TGG - chr1_4:71469861-71469880 None:intergenic 50.0%
GGGACTTCATGGATCCAAGA+TGG - chr1_4:71470156-71470175 None:intergenic 50.0%
GGATCCATGAAGTCCCACAT+TGG + chr1_4:71470160-71470179 Msa0182680:intron 50.0%
CTAGCCAATGTGGGACTTCA+TGG - chr1_4:71470167-71470186 None:intergenic 50.0%
CAAGCTAGCATTACCCAGCT+CGG - chr1_4:71470240-71470259 None:intergenic 50.0%
! ACTGCTGCTTCCTTGGGTTC+TGG - chr1_4:71469854-71469873 None:intergenic 55.0%
TGCTGGTATGTTCTCCGAGC+TGG + chr1_4:71470223-71470242 Msa0182680:intron 55.0%
GCTGGTATGTTCTCCGAGCT+GGG + chr1_4:71470224-71470243 Msa0182680:intron 55.0%
CAGGGATGCCCAAGTTAGCA+AGG - chr1_4:71470346-71470365 None:intergenic 55.0%
CTTGCTAACTTGGGCATCCC+TGG + chr1_4:71470344-71470363 Msa0182680:intron 55.0%
! ACTGCTGCTTCCTTGGGTTC+TGG - chr1_4:71469854-71469873 None:intergenic 55.0%
TGCTGGTATGTTCTCCGAGC+TGG + chr1_4:71470223-71470242 Msa0182680:intron 55.0%
GCTGGTATGTTCTCCGAGCT+GGG + chr1_4:71470224-71470243 Msa0182680:intron 55.0%
CAGGGATGCCCAAGTTAGCA+AGG - chr1_4:71470346-71470365 None:intergenic 55.0%
CTTGCTAACTTGGGCATCCC+TGG + chr1_4:71470344-71470363 Msa0182680:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 71469779 71471280 71469779 ID=Msa0182680;Name=Msa0182680
chr1_4 mRNA 71469779 71471280 71469779 ID=Msa0182680-mRNA-1;Parent=Msa0182680;Name=Msa0182680-mRNA-1;_AED=0.39;_eAED=0.55;_QI=0|0|0.5|1|1|1|2|195|73
chr1_4 exon 71469779 71469886 71469779 ID=Msa0182680-mRNA-1:exon:23915;Parent=Msa0182680-mRNA-1
chr1_4 exon 71470972 71471280 71470972 ID=Msa0182680-mRNA-1:exon:23916;Parent=Msa0182680-mRNA-1
chr1_4 CDS 71469779 71469886 71469779 ID=Msa0182680-mRNA-1:cds;Parent=Msa0182680-mRNA-1
chr1_4 CDS 71470972 71471085 71470972 ID=Msa0182680-mRNA-1:cds;Parent=Msa0182680-mRNA-1
chr1_4 three_prime_UTR 71471086 71471280 71471086 ID=Msa0182680-mRNA-1:three_prime_utr;Parent=Msa0182680-mRNA-1
Gene Sequence

>Msa0182680

ATGGCTGCTTTAGGGCCTCTATATGGATTTGGTTACCAGACTTTCTTATTAGAGTCTGCTTTGGGAACAAAACCAGAACCCAAGGAAGCAGCAGTAGCAAGTTATAATAAGGAAGATATTCAAGTTTGTCAAAATGTAATTTCAGACATAACAAATGCAGAGGTTGAGACACCATTTTCAACAAGTCACGTTGATTGGAAGAAGACATTTGGGTGCAACTAA

Protein sequence

>Msa0182680

MAALGPLYGFGYQTFLLESALGTKPEPKEAAVASYNKEDIQVCQNVISDITNAEVETPFSTSHVDWKKTFGCN*