AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75231


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75231.t1 MTR_5g042420 97.080 137 3 1 1 137 1 136 1.01e-92 267
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75231.t1 AT5G43750 48.413 126 63 1 12 137 13 136 2.42e-35 122

Find 36 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAATTTCAGGAACATTT+TGG 0.124367 1.4:-25307820 MS.gene75231:intron
TTCCCACCAGCCATTGCTGC+TGG 0.238546 1.4:-25307338 MS.gene75231:CDS
CTCATGCAGGGCTTATTTCA+TGG 0.312679 1.4:-25307364 MS.gene75231:intron
GAATGCCATTAGTGCGAAAT+TGG 0.318148 1.4:+25308615 None:intergenic
CAATTAACATTTAGAATTAC+CGG 0.341227 1.4:+25307299 None:intergenic
GTTGACCAATTTCGCACTAA+TGG 0.350257 1.4:-25308620 MS.gene75231:CDS
GGTCAACAGGATCTTCATTA+AGG 0.351255 1.4:+25308636 None:intergenic
TTTGAGTATGGAATATTTGA+TGG 0.360719 1.4:-25308479 MS.gene75231:CDS
CCAACACCACTCAACTTTCA+TGG 0.373078 1.4:-25308734 MS.gene75231:CDS
ACAAAATTCTTGTTGAATGC+TGG 0.374097 1.4:-25308680 MS.gene75231:CDS
ATATAGGAAGAATTTGAGTA+TGG 0.403141 1.4:-25308491 MS.gene75231:intron
TGGGGTATTCTTCGATGTTC+CGG 0.405466 1.4:-25307318 MS.gene75231:CDS
TCCCACCAGCCATTGCTGCT+GGG 0.411279 1.4:-25307337 MS.gene75231:CDS
TGTTGAGTTATTGTTACTCT+TGG 0.429120 1.4:+25308700 None:intergenic
TTAGTGCGAAATTGGTCAAC+AGG 0.452985 1.4:+25308623 None:intergenic
AGTTTGGATGGAGTGATGAT+AGG 0.472201 1.4:+25308779 None:intergenic
CCCACCAGCCATTGCTGCTG+GGG 0.474226 1.4:-25307336 MS.gene75231:CDS
GGTCATCATACTTTCAATGA+AGG 0.504424 1.4:-25308458 MS.gene75231:CDS
TTGGATGGAGTGATGATAGG+AGG 0.525418 1.4:+25308782 None:intergenic
CCATGAAAGTTGAGTGGTGT+TGG 0.531670 1.4:+25308734 None:intergenic
AATGAAGGAGAAGAAAAGAA+AGG 0.531960 1.4:-25308443 MS.gene75231:intron
ACAGGATCTTCATTAAGGTC+AGG 0.545810 1.4:+25308641 None:intergenic
GAGTGGTGTTGGAGATGAGA+AGG 0.548650 1.4:+25308745 None:intergenic
TGGATGGAGTGATGATAGGA+GGG 0.548771 1.4:+25308783 None:intergenic
AACATACCTTGAAATCCAGT+GGG 0.550957 1.4:+25307761 None:intergenic
AATACCCCAGCAGCAATGGC+TGG 0.556419 1.4:+25307332 None:intergenic
GAAGAATACCCCAGCAGCAA+TGG 0.556648 1.4:+25307328 None:intergenic
CCCCAGCAGCAATGGCTGGT+GGG 0.565163 1.4:+25307336 None:intergenic
ACCCCAGCAGCAATGGCTGG+TGG 0.569943 1.4:+25307335 None:intergenic
ATGGATGATGTAATAGCAGT+AGG 0.572923 1.4:-25307788 MS.gene75231:CDS
AGGAGGGACAAGTGAGAGTG+AGG 0.582918 1.4:+25308799 None:intergenic
AAACATACCTTGAAATCCAG+TGG 0.629247 1.4:+25307760 None:intergenic
CATACCTTGAAATCCAGTGG+GGG 0.685937 1.4:+25307763 None:intergenic
ATACCTTGAAATCCAGTGGG+GGG 0.705939 1.4:+25307764 None:intergenic
ACATACCTTGAAATCCAGTG+GGG 0.718019 1.4:+25307762 None:intergenic
AATATGCCATGAAAGTTGAG+TGG 0.727294 1.4:+25308728 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTAAAATATAAATAA+AGG + chr1.4:25307994-25308013 None:intergenic 0.0%
!! AAAATTAAAATATAAATAAA+GGG + chr1.4:25307993-25308012 None:intergenic 0.0%
!!! TATTTATATTTTAATTTTTA+AGG - chr1.4:25307995-25308014 MS.gene75231:intron 0.0%
!! AGTTAGAAAATTATATATAT+TGG + chr1.4:25308384-25308403 None:intergenic 10.0%
!! TAAATATGAAAAAAAAATAC+AGG + chr1.4:25307523-25307542 None:intergenic 10.0%
!!! ATATTTTTATTTTGAAAAAC+TGG - chr1.4:25307799-25307818 MS.gene75231:CDS 10.0%
!!! ATTTTTAAGGAATTATTACT+AGG - chr1.4:25308008-25308027 MS.gene75231:intron 15.0%
!! ATATGAAAAAAAAATACAGG+TGG + chr1.4:25307520-25307539 None:intergenic 20.0%
!! TTCATAATGTTAGAATGATT+AGG - chr1.4:25308075-25308094 MS.gene75231:intron 20.0%
!!! CAATTAACATTTAGAATTAC+CGG + chr1.4:25308814-25308833 None:intergenic 20.0%
!!! CTTTGTTTTTTAATGGAAAT+TGG - chr1.4:25307749-25307768 MS.gene75231:intron 20.0%
!!! TCAATCACTTTGTTTTTTAA+TGG - chr1.4:25307742-25307761 MS.gene75231:intron 20.0%
!!! TTGTTTTGTGTTGATTATAT+AGG - chr1.4:25307603-25307622 MS.gene75231:intron 20.0%
! AAAGTTTGAATTTCGATTGA+AGG - chr1.4:25308169-25308188 MS.gene75231:intron 25.0%
! AATATAAATAAAGGGGAAAC+AGG + chr1.4:25307985-25308004 None:intergenic 25.0%
! AATGATTACAGTCAAAATTG+TGG + chr1.4:25308694-25308713 None:intergenic 25.0%
! ACTAATGAATAAATAAGTCG+TGG - chr1.4:25308499-25308518 MS.gene75231:intron 25.0%
! ATATAAATAAAGGGGAAACA+GGG + chr1.4:25307984-25308003 None:intergenic 25.0%
! ATATAGGAAGAATTTGAGTA+TGG - chr1.4:25307619-25307638 MS.gene75231:intron 25.0%
! TTGCAACAGTAAAATTTGAT+GGG - chr1.4:25308041-25308060 MS.gene75231:intron 25.0%
! TTTGAGTATGGAATATTTGA+TGG - chr1.4:25307631-25307650 MS.gene75231:intron 25.0%
!! AATTTTCATTGTATTGTCGA+AGG - chr1.4:25307854-25307873 MS.gene75231:intron 25.0%
!! GTTAATTTCAGGAACATTTT+GGG - chr1.4:25308291-25308310 MS.gene75231:intron 25.0%
!! TGTTAATTTCAGGAACATTT+TGG - chr1.4:25308290-25308309 MS.gene75231:intron 25.0%
!! TTAATTTCAGGAACATTTTG+GGG - chr1.4:25308292-25308311 MS.gene75231:intron 25.0%
!!! CATGTTTTTAAAAACTTGAC+GGG - chr1.4:25308415-25308434 MS.gene75231:intron 25.0%
!!! GCATGTTTTTAAAAACTTGA+CGG - chr1.4:25308414-25308433 MS.gene75231:intron 25.0%
!!! TTTTGAAAAACTGGAGTATA+AGG - chr1.4:25307808-25307827 MS.gene75231:CDS 25.0%
AATAAGATTACCTACAAAGG+AGG - chr1.4:25308192-25308211 MS.gene75231:intron 30.0%
AATGAAGGAGAAGAAAAGAA+AGG - chr1.4:25307667-25307686 MS.gene75231:intron 30.0%
ACAAAATTCTTGTTGAATGC+TGG - chr1.4:25307430-25307449 MS.gene75231:intron 30.0%
AGGAATAAGATTACCTACAA+AGG - chr1.4:25308189-25308208 MS.gene75231:intron 30.0%
ATAAGATTACCTACAAAGGA+GGG - chr1.4:25308193-25308212 MS.gene75231:intron 30.0%
CTTGCAACAGTAAAATTTGA+TGG - chr1.4:25308040-25308059 MS.gene75231:intron 30.0%
TATAAATAAAGGGGAAACAG+GGG + chr1.4:25307983-25308002 None:intergenic 30.0%
TCTAATTTGATTCTCATGCA+GGG - chr1.4:25308734-25308753 MS.gene75231:CDS 30.0%
TGTTGAGTTATTGTTACTCT+TGG + chr1.4:25307413-25307432 None:intergenic 30.0%
TTCTAATTTGATTCTCATGC+AGG - chr1.4:25308733-25308752 MS.gene75231:CDS 30.0%
TTTCCTAACAAAGTTCATGA+TGG - chr1.4:25308266-25308285 MS.gene75231:intron 30.0%
!! TCATGATGGATGTTAATTTC+AGG - chr1.4:25308280-25308299 MS.gene75231:intron 30.0%
!!! TTTCATGTTGCTGATTTTTG+TGG + chr1.4:25308646-25308665 None:intergenic 30.0%
AAACAGATACAGACTTAGTC+AGG + chr1.4:25308248-25308267 None:intergenic 35.0%
AAACATACCTTGAAATCCAG+TGG + chr1.4:25308353-25308372 None:intergenic 35.0%
AACATACCTTGAAATCCAGT+GGG + chr1.4:25308352-25308371 None:intergenic 35.0%
AATATGCCATGAAAGTTGAG+TGG + chr1.4:25307385-25307404 None:intergenic 35.0%
ACAGTCAAAATTGTGGTCAA+GGG + chr1.4:25308687-25308706 None:intergenic 35.0%
ACGAGATATTAAGAGAGTGA+TGG - chr1.4:25307953-25307972 MS.gene75231:intron 35.0%
ATGGATGATGTAATAGCAGT+AGG - chr1.4:25308322-25308341 MS.gene75231:intron 35.0%
CATCCATCATGAACTTTGTT+AGG + chr1.4:25308272-25308291 None:intergenic 35.0%
GGTCATCATACTTTCAATGA+AGG - chr1.4:25307652-25307671 MS.gene75231:intron 35.0%
TAATGGAAATTGGAGCATGT+TGG - chr1.4:25307759-25307778 MS.gene75231:intron 35.0%
TACAGTCAAAATTGTGGTCA+AGG + chr1.4:25308688-25308707 None:intergenic 35.0%
TATCGCTGATATTGTGATCA+AGG + chr1.4:25308568-25308587 None:intergenic 35.0%
! ACCACAATTTGGAGGTTTTA+CGG + chr1.4:25308450-25308469 None:intergenic 35.0%
!!! AACATTTTGGGGTGCAATTA+TGG - chr1.4:25308303-25308322 MS.gene75231:intron 35.0%
!!! GAAGGATTTTGAAGTGAGTT+TGG + chr1.4:25307350-25307369 None:intergenic 35.0%
!!! GATTTTGAAGTGAGTTTGGA+TGG + chr1.4:25307346-25307365 None:intergenic 35.0%
ACAGGATCTTCATTAAGGTC+AGG + chr1.4:25307472-25307491 None:intergenic 40.0%
ACATACCTTGAAATCCAGTG+GGG + chr1.4:25308351-25308370 None:intergenic 40.0%
AGAAAAATGCACCTACCAAC+AGG - chr1.4:25308101-25308120 MS.gene75231:intron 40.0%
AGTTTGGATGGAGTGATGAT+AGG + chr1.4:25307334-25307353 None:intergenic 40.0%
ATCTCGTTTGTTTGTCCATG+AGG + chr1.4:25307940-25307959 None:intergenic 40.0%
CTAACTGAACCACAATTTGG+AGG + chr1.4:25308458-25308477 None:intergenic 40.0%
GAAAAATGCACCTACCAACA+GGG - chr1.4:25308102-25308121 MS.gene75231:intron 40.0%
GAATGCCATTAGTGCGAAAT+TGG + chr1.4:25307498-25307517 None:intergenic 40.0%
GAGCTAACTGAACCACAATT+TGG + chr1.4:25308461-25308480 None:intergenic 40.0%
GCAATGGCAAGAGTTTGAAA+TGG + chr1.4:25307904-25307923 None:intergenic 40.0%
GGTCAACAGGATCTTCATTA+AGG + chr1.4:25307477-25307496 None:intergenic 40.0%
GTTGACCAATTTCGCACTAA+TGG - chr1.4:25307490-25307509 MS.gene75231:intron 40.0%
TACAAAGGAGGGAATCTATG+AGG - chr1.4:25308204-25308223 MS.gene75231:intron 40.0%
TACAGACTTAGTCAGGAATC+AGG + chr1.4:25308241-25308260 None:intergenic 40.0%
TCATAGATTCCCTCCTTTGT+AGG + chr1.4:25308205-25308224 None:intergenic 40.0%
TGTGGTTCAGTTAGCTCAAA+CGG - chr1.4:25308464-25308483 MS.gene75231:CDS 40.0%
TTAGTGCGAAATTGGTCAAC+AGG + chr1.4:25307490-25307509 None:intergenic 40.0%
! CCACAATTTGGAGGTTTTAC+GGG + chr1.4:25308449-25308468 None:intergenic 40.0%
ACAGGGTTGAATCGAACGAA+AGG - chr1.4:25308119-25308138 MS.gene75231:intron 45.0%
AGGGTTGAATCGAACGAAAG+GGG - chr1.4:25308121-25308140 MS.gene75231:intron 45.0%
AGGTGTGACAACAATGGCAA+TGG + chr1.4:25307920-25307939 None:intergenic 45.0%
ATACCTTGAAATCCAGTGGG+GGG + chr1.4:25308349-25308368 None:intergenic 45.0%
CAGGGTTGAATCGAACGAAA+GGG - chr1.4:25308120-25308139 MS.gene75231:intron 45.0%
CATACCTTGAAATCCAGTGG+GGG + chr1.4:25308350-25308369 None:intergenic 45.0%
CCAACACCACTCAACTTTCA+TGG - chr1.4:25307376-25307395 MS.gene75231:intron 45.0%
CCATGAAAGTTGAGTGGTGT+TGG + chr1.4:25307379-25307398 None:intergenic 45.0%
CCCGTAAAACCTCCAAATTG+TGG - chr1.4:25308446-25308465 MS.gene75231:CDS 45.0%
CTCATGCAGGGCTTATTTCA+TGG - chr1.4:25308746-25308765 MS.gene75231:CDS 45.0%
TCAAAATTGTGGTCAAGGGC+CGG + chr1.4:25308683-25308702 None:intergenic 45.0%
TCCATGAGGTGTGACAACAA+TGG + chr1.4:25307926-25307945 None:intergenic 45.0%
TCGTGGTTGTGTTGCAATTG+CGG - chr1.4:25308516-25308535 MS.gene75231:intron 45.0%
TGGATGGAGTGATGATAGGA+GGG + chr1.4:25307330-25307349 None:intergenic 45.0%
TTCGATTCAACCCTGTTGGT+AGG + chr1.4:25308115-25308134 None:intergenic 45.0%
TTCGTTCGATTCAACCCTGT+TGG + chr1.4:25308119-25308138 None:intergenic 45.0%
TTGGAGCATGTTGGTACTCA+TGG - chr1.4:25307768-25307787 MS.gene75231:CDS 45.0%
TTGGATGGAGTGATGATAGG+AGG + chr1.4:25307331-25307350 None:intergenic 45.0%
! TGGGGTATTCTTCGATGTTC+CGG - chr1.4:25308792-25308811 MS.gene75231:CDS 45.0%
!! AAATTAAAATATAAATAAAG+GGG + chr1.4:25307992-25308011 None:intergenic 5.0%
GAAGAATACCCCAGCAGCAA+TGG + chr1.4:25308785-25308804 None:intergenic 50.0%
GCCATTGTTGTCACACCTCA+TGG - chr1.4:25307922-25307941 MS.gene75231:intron 50.0%
GGTCGATACTGCCTCAATCT+CGG + chr1.4:25308625-25308644 None:intergenic 50.0%
! GAGTGGTGTTGGAGATGAGA+AGG + chr1.4:25307368-25307387 None:intergenic 50.0%
! TGAAAGCTTGCCACTTGAGC+CGG - chr1.4:25308661-25308680 MS.gene75231:CDS 50.0%
AATACCCCAGCAGCAATGGC+TGG + chr1.4:25308781-25308800 None:intergenic 55.0%
AGGAGGGACAAGTGAGAGTG+AGG + chr1.4:25307314-25307333 None:intergenic 55.0%
CTGCACTGCAACCGAGATTG+AGG - chr1.4:25308611-25308630 MS.gene75231:CDS 55.0%
TCTCGGTTGCAGTGCAGTTG+TGG + chr1.4:25308608-25308627 None:intergenic 55.0%
ATAGCAGTAGGACCCCCCAC+TGG - chr1.4:25308334-25308353 MS.gene75231:intron 60.0%
GGACCCCCCACTGGATTTCA+AGG - chr1.4:25308343-25308362 MS.gene75231:intron 60.0%
TCCCACCAGCCATTGCTGCT+GGG - chr1.4:25308773-25308792 MS.gene75231:CDS 60.0%
TTCCCACCAGCCATTGCTGC+TGG - chr1.4:25308772-25308791 MS.gene75231:CDS 60.0%
ACCCCAGCAGCAATGGCTGG+TGG + chr1.4:25308778-25308797 None:intergenic 65.0%
CCCACCAGCCATTGCTGCTG+GGG - chr1.4:25308774-25308793 MS.gene75231:CDS 65.0%
CCCCAGCAGCAATGGCTGGT+GGG + chr1.4:25308777-25308796 None:intergenic 65.0%
! TGGTCAAGGGCCGGCTCAAG+TGG + chr1.4:25308674-25308693 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 25307298 25308834 25307298 ID=MS.gene75231
chr1.4 mRNA 25307298 25308834 25307298 ID=MS.gene75231.t1;Parent=MS.gene75231
chr1.4 exon 25308610 25308834 25308610 ID=MS.gene75231.t1.exon1;Parent=MS.gene75231.t1
chr1.4 CDS 25308610 25308834 25308610 ID=cds.MS.gene75231.t1;Parent=MS.gene75231.t1
chr1.4 exon 25308444 25308507 25308444 ID=MS.gene75231.t1.exon2;Parent=MS.gene75231.t1
chr1.4 CDS 25308444 25308507 25308444 ID=cds.MS.gene75231.t1;Parent=MS.gene75231.t1
chr1.4 exon 25307768 25307830 25307768 ID=MS.gene75231.t1.exon3;Parent=MS.gene75231.t1
chr1.4 CDS 25307768 25307830 25307768 ID=cds.MS.gene75231.t1;Parent=MS.gene75231.t1
chr1.4 exon 25307298 25307377 25307298 ID=MS.gene75231.t1.exon4;Parent=MS.gene75231.t1
chr1.4 CDS 25307298 25307377 25307298 ID=cds.MS.gene75231.t1;Parent=MS.gene75231.t1
Gene Sequence

>MS.gene75231

ATGGCGCTATGTTCCTCACTCTCACTTGTCCCTCCTATCATCACTCCATCCAAACTCACTTCAAAATCCTTCTCATCTCCAACACCACTCAACTTTCATGGCATATTCACTACCAAGAGTAACAATAACTCAACAAAATTCTTGTTGAATGCTGGTTTCAATCTTATTGAGCCTGACCTTAATGAAGATCCTGTTGACCAATTTCGCACTAATGGCATTCCACCTGTATTTTTTTTTCATATTTATACTTCATTTTGTTTAATTACTTTTTTTTACTATAGTTTTGTTTTTGTGATGAGATTTGTTTGTTTTGTGTTGATTATATAGGAAGAATTTGAGTATGGAATATTTGATGGTCATCATACTTTCAATGAAGGAGAAGAAAAGAAAGGTATTTGTTTTTTCATTGCTTACTTTCTTAGTTTTACAGTATCTTTTCATTTTTCAATCACTTTGTTTTTTAATGGAAATTGGAGCATGTTGGTACTCATGGTAATTGAGATATTTTTATTTTGAAAAACTGGAGTATAAGGAAATTGTTATTAATTAAAGAGACAATTTTCATTGTATTGTCGAAGGATTTGAACTCGATGTCTTATGTTACCATTTCAAACTCTTGCCATTGCCATTGTTGTCACACCTCATGGACAAACAAACGAGATATTAAGAGAGTGATGGATTTCCCCTGTTTCCCCTTTATTTATATTTTAATTTTTAAGGAATTATTACTAGGTGTTTGTTTCTTGCAACAGTAAAATTTGATGGGAATTATTCAAATTCATAATGTTAGAATGATTAGGAAAAGAAAAATGCACCTACCAACAGGGTTGAATCGAACGAAAGGGGTTAGACTTCTGCAAGATGATCAACAAAAGTTTGAATTTCGATTGAAGGAATAAGATTACCTACAAAGGAGGGAATCTATGAGGACAAAAATGATCCTGATTCCTGACTAAGTCTGTATCTGTTTTCCTAACAAAGTTCATGATGGATGTTAATTTCAGGAACATTTTGGGGTGCAATTATGGATGATGTAATAGCAGTAGGACCCCCCACTGGATTTCAAGGTATGTTTTACTTGCACCAATATATATAATTTTCTAACTTGTATTACAAGCATGTTTTTAAAAACTTGACGGGTGATTGAGCCCGTAAAACCTCCAAATTGTGGTTCAGTTAGCTCAAACGGTTGATCTGAATGACTAATGAATAAATAAGTCGTGGTTGTGTTGCAATTGCGGAGTTTTATAAACAGAAATGTTTGCGACCTTGATCACAATATCAGCGATATCGAAGTATTTTTGTCTCCACAACTGCACTGCAACCGAGATTGAGGCAGTATCGACCACAAAAATCAGCAACATGAAAGCTTGCCACTTGAGCCGGCCCTTGACCACAATTTTGACTGTAATCATTGATTTCGATTATTGTTTAATTCTAATTTGATTCTCATGCAGGGCTTATTTCATGGCTCTTCCCACCAGCCATTGCTGCTGGGGTATTCTTCGATGTTCCGGTAATTCTAAATGTTAATTGA

Protein sequence

>MS.gene75231.t1

MALCSSLSLVPPIITPSKLTSKSFSSPTPLNFHGIFTTKSNNNSTKFLLNAGFNLIEPDLNEDPVDQFRTNGIPPEEFEYGIFDGHHTFNEGEEKKGTFWGAIMDDVIAVGPPTGFQGLISWLFPPAIAAGVFFDVPVILNVN