AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067060.t1 MTR_3g086230 99.492 197 0 1 1 196 1 197 3.13e-138 384
MS.gene067060.t1 MTR_8g089610 65.196 204 58 9 1 194 1 201 1.02e-62 193
MS.gene067060.t1 MTR_2g046810 51.634 153 43 10 8 151 1 131 3.60e-24 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067060.t1 AT1G67740 52.105 190 73 5 12 195 10 187 2.09e-44 146

Find 49 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGTGGACTTGCAGCTTC+AGG 0.300451 3.4:-80386836 MS.gene067060:CDS
GTTGGGCTTGGACTTGGACT+TGG 0.325974 3.4:-80386854 MS.gene067060:CDS
GTGATTGTTGGGCTTGGACT+TGG 0.338557 3.4:-80386860 MS.gene067060:CDS
TGACACAAAGTTGGTGTTCT+TGG 0.338685 3.4:+80387090 None:intergenic
TCACCTTCACTTGCAATTGC+AGG 0.361555 3.4:-80387070 MS.gene067060:CDS
CTGATCTCATCCTGTTGATC+TGG 0.394420 3.4:+80386672 None:intergenic
CGTTACGCATACGGTTGATT+TGG 0.394692 3.4:+80386891 None:intergenic
TTTGTTGTTGCACCAGCTAT+AGG 0.399173 3.4:-80386950 MS.gene067060:CDS
GAAGCATCTGCTAGTGAGAT+TGG 0.403127 3.4:-80386803 MS.gene067060:CDS
ACTTGGGTAGGCCTGTGTTT+AGG 0.404849 3.4:+80387197 None:intergenic
ACGCAAAAGGTGTGATTGTT+GGG 0.409047 3.4:-80386871 MS.gene067060:CDS
CTGCTCTGAACCAGATCAAC+AGG 0.409433 3.4:-80386682 MS.gene067060:CDS
ATGCTGCTGGAAGTGACAAC+AGG 0.409635 3.4:-80386769 MS.gene067060:CDS
AACCGTATGCGTAACGCAAA+AGG 0.427923 3.4:-80386884 MS.gene067060:CDS
TTGTTGCACCAGCTATCCTT+TGG 0.431369 3.4:-80386727 MS.gene067060:CDS
GGGCTTGGACTTGGACTTGG+TGG 0.436653 3.4:-80386851 MS.gene067060:CDS
TGTTGCACCAGCTATCCTTT+GGG 0.438183 3.4:-80386726 MS.gene067060:CDS
AACGCAAAAGGTGTGATTGT+TGG 0.441975 3.4:-80386872 MS.gene067060:CDS
GGTTTGATTGCTGATGCTGC+TGG 0.461729 3.4:-80386782 MS.gene067060:CDS
TTGTTGCACCAGCTATAGGT+TGG 0.462026 3.4:-80386946 MS.gene067060:CDS
TGTTGCACCAGCTATAGGTT+GGG 0.466375 3.4:-80386945 MS.gene067060:CDS
AAAGGTGTGATTGTTGGGCT+TGG 0.466916 3.4:-80386866 MS.gene067060:CDS
CGGTTGATTTGGTTCAAAGC+TGG 0.475315 3.4:+80386902 None:intergenic
CTGTTGATCTGGTTCAGAGC+AGG 0.484163 3.4:+80386683 None:intergenic
TTGGTTGAGACAAGACCCTT+TGG 0.496099 3.4:+80387109 None:intergenic
TACAGCACCCAAAGGATAGC+TGG 0.516105 3.4:+80386719 None:intergenic
TGGTAGGTTCTGCATGGTGG+TGG 0.518556 3.4:+80387129 None:intergenic
AAGCCGCGTCGGAGGTTGCA+AGG 0.534226 3.4:+80387032 None:intergenic
TGGACTTGCAGCTTCAGGTA+TGG 0.535553 3.4:-80386831 MS.gene067060:CDS
AGCTGCGAAAGCCGCGTCGG+AGG 0.541223 3.4:+80387024 None:intergenic
ACCCTTTGGTAGGTTCTGCA+TGG 0.542276 3.4:+80387123 None:intergenic
AACAAAACCCAACCTATAGC+TGG 0.551151 3.4:+80386938 None:intergenic
AGCCGCGTCGGAGGTTGCAA+GGG 0.564916 3.4:+80387033 None:intergenic
TTCATCAATGGCGGCAACAA+TGG 0.573337 3.4:-80387242 None:intergenic
ACCATGCAGAACCTACCAAA+GGG 0.580172 3.4:-80387124 MS.gene067060:CDS
TGCTGCTGGAAGTGACAACA+GGG 0.593134 3.4:-80386768 MS.gene067060:CDS
TTGAGACAAGACCCTTTGGT+AGG 0.600634 3.4:+80387113 None:intergenic
CAAATAGCAGAAATAGCAGA+AGG 0.607721 3.4:-80386998 MS.gene067060:CDS
TGTTGATGTTAGCTTAGTAG+TGG 0.612701 3.4:+80387159 None:intergenic
CTTTGGTAGGTTCTGCATGG+TGG 0.614099 3.4:+80387126 None:intergenic
TTGAGCTGCGAAAGCCGCGT+CGG 0.615667 3.4:+80387021 None:intergenic
CACCATGCAGAACCTACCAA+AGG 0.623574 3.4:-80387125 MS.gene067060:CDS
AACACAAAGTGCCTAAACAC+AGG 0.637457 3.4:-80387208 MS.gene067060:CDS
ATCCCTTGCAACCTCCGACG+CGG 0.643167 3.4:-80387035 MS.gene067060:CDS
AAATATTGTACAGCACCCAA+AGG 0.643861 3.4:+80386711 None:intergenic
AAGTGAAGGTGACACAAAGT+TGG 0.674791 3.4:+80387081 None:intergenic
ATAGCAGAAGGTGATAACAG+AGG 0.682957 3.4:-80386986 MS.gene067060:CDS
GCTCCTGCAATTGCAAGTGA+AGG 0.683304 3.4:+80387067 None:intergenic
GCTGCTGGAAGTGACAACAG+GGG 0.839610 3.4:-80386767 MS.gene067060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAATATTGTACAGCACCCAA+AGG + chr3.4:80387194-80387213 None:intergenic 35.0%
CAAATAGCAGAAATAGCAGA+AGG - chr3.4:80386904-80386923 MS.gene067060:CDS 35.0%
! GTGCTAAGATTTTGCAACTT+GGG + chr3.4:80386724-80386743 None:intergenic 35.0%
! TAAGATTTTGCAACTTGGGT+AGG + chr3.4:80386720-80386739 None:intergenic 35.0%
! TGTTGATGTTAGCTTAGTAG+TGG + chr3.4:80386746-80386765 None:intergenic 35.0%
AACAAAACCCAACCTATAGC+TGG + chr3.4:80386967-80386986 None:intergenic 40.0%
AAGTGAAGGTGACACAAAGT+TGG + chr3.4:80386824-80386843 None:intergenic 40.0%
ATAGCAGAAGGTGATAACAG+AGG - chr3.4:80386916-80386935 MS.gene067060:CDS 40.0%
! AACACAAAGTGCCTAAACAC+AGG - chr3.4:80386694-80386713 MS.gene067060:CDS 40.0%
! AACGCAAAAGGTGTGATTGT+TGG - chr3.4:80387030-80387049 MS.gene067060:CDS 40.0%
! ACGCAAAAGGTGTGATTGTT+GGG - chr3.4:80387031-80387050 MS.gene067060:CDS 40.0%
! GGTGCTAAGATTTTGCAACT+TGG + chr3.4:80386725-80386744 None:intergenic 40.0%
!! TGACACAAAGTTGGTGTTCT+TGG + chr3.4:80386815-80386834 None:intergenic 40.0%
!! TTTGTTGTTGCACCAGCTAT+AGG - chr3.4:80386952-80386971 MS.gene067060:CDS 40.0%
AACCGTATGCGTAACGCAAA+AGG - chr3.4:80387018-80387037 MS.gene067060:CDS 45.0%
ACCATGCAGAACCTACCAAA+GGG - chr3.4:80386778-80386797 MS.gene067060:CDS 45.0%
CGTTACGCATACGGTTGATT+TGG + chr3.4:80387014-80387033 None:intergenic 45.0%
CTGATCTCATCCTGTTGATC+TGG + chr3.4:80387233-80387252 None:intergenic 45.0%
TCACCTTCACTTGCAATTGC+AGG - chr3.4:80386832-80386851 MS.gene067060:CDS 45.0%
TGTTGCACCAGCTATAGGTT+GGG - chr3.4:80386957-80386976 MS.gene067060:CDS 45.0%
TGTTGCACCAGCTATCCTTT+GGG - chr3.4:80387176-80387195 MS.gene067060:CDS 45.0%
TTGAGACAAGACCCTTTGGT+AGG + chr3.4:80386792-80386811 None:intergenic 45.0%
TTGGTTGAGACAAGACCCTT+TGG + chr3.4:80386796-80386815 None:intergenic 45.0%
TTGTTGCACCAGCTATAGGT+TGG - chr3.4:80386956-80386975 MS.gene067060:CDS 45.0%
TTGTTGCACCAGCTATCCTT+TGG - chr3.4:80387175-80387194 MS.gene067060:CDS 45.0%
! AAAGGTGTGATTGTTGGGCT+TGG - chr3.4:80387036-80387055 MS.gene067060:CDS 45.0%
! CACCTTTTGCGTTACGCATA+CGG + chr3.4:80387023-80387042 None:intergenic 45.0%
! GAAGCATCTGCTAGTGAGAT+TGG - chr3.4:80387099-80387118 MS.gene067060:CDS 45.0%
!! CGGTTGATTTGGTTCAAAGC+TGG + chr3.4:80387003-80387022 None:intergenic 45.0%
ACCCTTTGGTAGGTTCTGCA+TGG + chr3.4:80386782-80386801 None:intergenic 50.0%
ACTTGGGTAGGCCTGTGTTT+AGG + chr3.4:80386708-80386727 None:intergenic 50.0%
ATGCTGCTGGAAGTGACAAC+AGG - chr3.4:80387133-80387152 MS.gene067060:CDS 50.0%
CACCATGCAGAACCTACCAA+AGG - chr3.4:80386777-80386796 MS.gene067060:CDS 50.0%
CTTTGGTAGGTTCTGCATGG+TGG + chr3.4:80386779-80386798 None:intergenic 50.0%
GCTCCTGCAATTGCAAGTGA+AGG + chr3.4:80386838-80386857 None:intergenic 50.0%
GGTTTGATTGCTGATGCTGC+TGG - chr3.4:80387120-80387139 MS.gene067060:CDS 50.0%
GTGATTGTTGGGCTTGGACT+TGG - chr3.4:80387042-80387061 MS.gene067060:CDS 50.0%
TACAGCACCCAAAGGATAGC+TGG + chr3.4:80387186-80387205 None:intergenic 50.0%
TGCTGCTGGAAGTGACAACA+GGG - chr3.4:80387134-80387153 MS.gene067060:CDS 50.0%
TGGACTTGCAGCTTCAGGTA+TGG - chr3.4:80387071-80387090 MS.gene067060:CDS 50.0%
! CTGCTCTGAACCAGATCAAC+AGG - chr3.4:80387220-80387239 MS.gene067060:CDS 50.0%
! CTGTTGATCTGGTTCAGAGC+AGG + chr3.4:80387222-80387241 None:intergenic 50.0%
GCTGCTGGAAGTGACAACAG+GGG - chr3.4:80387135-80387154 MS.gene067060:CDS 55.0%
GTTGGGCTTGGACTTGGACT+TGG - chr3.4:80387048-80387067 MS.gene067060:CDS 55.0%
TGGTAGGTTCTGCATGGTGG+TGG + chr3.4:80386776-80386795 None:intergenic 55.0%
!! CTTGGTGGACTTGCAGCTTC+AGG - chr3.4:80387066-80387085 MS.gene067060:CDS 55.0%
ATCCCTTGCAACCTCCGACG+CGG - chr3.4:80386867-80386886 MS.gene067060:CDS 60.0%
GGGCTTGGACTTGGACTTGG+TGG - chr3.4:80387051-80387070 MS.gene067060:CDS 60.0%
TTGAGCTGCGAAAGCCGCGT+CGG + chr3.4:80386884-80386903 None:intergenic 60.0%
AAGCCGCGTCGGAGGTTGCA+AGG + chr3.4:80386873-80386892 None:intergenic 65.0%
AGCCGCGTCGGAGGTTGCAA+GGG + chr3.4:80386872-80386891 None:intergenic 65.0%
AGCTGCGAAAGCCGCGTCGG+AGG + chr3.4:80386881-80386900 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 80386667 80387257 80386667 ID=MS.gene067060
chr3.4 mRNA 80386667 80387257 80386667 ID=MS.gene067060.t1;Parent=MS.gene067060
chr3.4 exon 80386667 80387257 80386667 ID=MS.gene067060.t1.exon1;Parent=MS.gene067060.t1
chr3.4 CDS 80386667 80387257 80386667 ID=cds.MS.gene067060.t1;Parent=MS.gene067060.t1
Gene Sequence

>MS.gene067060

ATGGCGGCAACAATGGCAATGATGATCAACACAAAGTGCCTAAACACAGGCCTACCCAAGTTGCAAAATCTTAGCACCACTACTAAGCTAACATCAACATGCAAAACCACCACCATGCAGAACCTACCAAAGGGTCTTGTCTCAACCAAGAACACCAACTTTGTGTCACCTTCACTTGCAATTGCAGGAGCAATATTTTCATCCCTTGCAACCTCCGACGCGGCTTTCGCAGCTCAACAAATAGCAGAAATAGCAGAAGGTGATAACAGAGGAACACTTCTGCTGTTTGTTGTTGCACCAGCTATAGGTTGGGTTTTGTTCAACATATTGCAGCCAGCTTTGAACCAAATCAACCGTATGCGTAACGCAAAAGGTGTGATTGTTGGGCTTGGACTTGGACTTGGTGGACTTGCAGCTTCAGGTATGGTGTCAGAAGCATCTGCTAGTGAGATTGGTTTGATTGCTGATGCTGCTGGAAGTGACAACAGGGGAACACTTCTGCTGTTTGTTGTTGCACCAGCTATCCTTTGGGTGCTGTACAATATTTTGCAGCCTGCTCTGAACCAGATCAACAGGATGAGATCAGATTAA

Protein sequence

>MS.gene067060.t1

MAATMAMMINTKCLNTGLPKLQNLSTTTKLTSTCKTTTMQNLPKGLVSTKNTNFVSPSLAIAGAIFSSLATSDAAFAAQQIAEIAEGDNRGTLLLFVVAPAIGWVLFNILQPALNQINRMRNAKGVIVGLGLGLGGLAASGMVSEASASEIGLIADAAGSDNRGTLLLFVVAPAILWVLYNILQPALNQINRMRSD