AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0695100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTGTCCTGTTCTCATTT+AGG 0.314248 4_4:-64534772 None:intergenic
CCAAGTTTCCAACACTCATT+TGG 0.337322 4_4:+64535160 Msa0695100:CDS
CCAAATGAGTGTTGGAAACT+TGG 0.351640 4_4:-64535160 None:intergenic
CAATAGTGCTGTCCTGTGTT+AGG 0.431189 4_4:+64535183 Msa0695100:CDS
CAGGACAATCAACAGTGAAC+AGG 0.475162 4_4:+64534784 Msa0695100:CDS
TGAGAGGCCTAAATGAGAAC+AGG 0.496589 4_4:+64534765 Msa0695100:CDS
CACTATTGCCAAATGAGTGT+TGG 0.524522 4_4:-64535168 None:intergenic
AGGAAAAGCATGAGCTTGAG+AGG 0.547191 4_4:+64534749 Msa0695100:CDS
TGTCTTTCGTATAAGATGAC+TGG 0.564549 4_4:+64535070 Msa0695100:intron
ACTTGGAATGTATAGACTTG+AGG 0.619589 4_4:-64535143 None:intergenic
GACATTTGCCATCCTAACAC+AGG 0.640305 4_4:-64535195 None:intergenic
AACCAAAATGACGAATGATG+AGG 0.641924 4_4:+64534729 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAACATATATATTGAAA+AGG - chr4_4:64534895-64534914 None:intergenic 15.0%
! TACATAGTTAAACTTAAGAG+TGG - chr4_4:64534981-64535000 None:intergenic 25.0%
TGAACAGGTTATTGTAAACT+TGG + chr4_4:64534799-64534818 Msa0695100:intron 30.0%
TCTTGTCAGATAATTACTTC+AGG - chr4_4:64534919-64534938 None:intergenic 30.0%
TGATTGTCCTGTTCTCATTT+AGG - chr4_4:64534775-64534794 None:intergenic 35.0%
!! TACAAAAACTGTTGGTGACT+TGG - chr4_4:64535046-64535065 None:intergenic 35.0%
GACAGTTCTACAAAAACTGT+TGG - chr4_4:64535054-64535073 None:intergenic 35.0%
TGTCTTTCGTATAAGATGAC+TGG + chr4_4:64535070-64535089 Msa0695100:intron 35.0%
ACTTGGAATGTATAGACTTG+AGG - chr4_4:64535146-64535165 None:intergenic 35.0%
GTTAAACTTAAGAGTGGCGT+GGG - chr4_4:64534975-64534994 None:intergenic 40.0%
AGTTAAACTTAAGAGTGGCG+TGG - chr4_4:64534976-64534995 None:intergenic 40.0%
!! CCAAATGAGTGTTGGAAACT+TGG - chr4_4:64535163-64535182 None:intergenic 40.0%
CCAAGTTTCCAACACTCATT+TGG + chr4_4:64535160-64535179 Msa0695100:CDS 40.0%
CACTATTGCCAAATGAGTGT+TGG - chr4_4:64535171-64535190 None:intergenic 40.0%
! AGGAAAAGCATGAGCTTGAG+AGG + chr4_4:64534749-64534768 Msa0695100:CDS 45.0%
TGAGAGGCCTAAATGAGAAC+AGG + chr4_4:64534765-64534784 Msa0695100:CDS 45.0%
CAGGACAATCAACAGTGAAC+AGG + chr4_4:64534784-64534803 Msa0695100:CDS 45.0%
ACTTAAGAGTGGCGTGGGTA+GGG - chr4_4:64534970-64534989 None:intergenic 50.0%
AACTTAAGAGTGGCGTGGGT+AGG - chr4_4:64534971-64534990 None:intergenic 50.0%
CTTAAGAGTGGCGTGGGTAG+GGG - chr4_4:64534969-64534988 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 64534743 64535204 64534743 ID=Msa0695100;Name=Msa0695100
chr4_4 mRNA 64534743 64535204 64534743 ID=Msa0695100-mRNA-1;Parent=Msa0695100;Name=Msa0695100-mRNA-1;_AED=0.81;_eAED=1.00;_QI=0|0|0|0.5|1|1|2|0|60
chr4_4 exon 64534743 64534805 64534743 ID=Msa0695100-mRNA-1:exon:18498;Parent=Msa0695100-mRNA-1
chr4_4 exon 64535085 64535204 64535085 ID=Msa0695100-mRNA-1:exon:18499;Parent=Msa0695100-mRNA-1
chr4_4 CDS 64534743 64534805 64534743 ID=Msa0695100-mRNA-1:cds;Parent=Msa0695100-mRNA-1
chr4_4 CDS 64535085 64535204 64535085 ID=Msa0695100-mRNA-1:cds;Parent=Msa0695100-mRNA-1
Gene Sequence

>Msa0695100

ATGATGAGGAAAAGCATGAGCTTGAGAGGCCTAAATGAGAACAGGACAATCAACAGTGAACAGATGACTGGTGTTGCAGAGACAAGTAAGTCTATTCAGCTCAAAACTCACAAAGTACTGTCCTCAAGTCTATACATTCCAAGTTTCCAACACTCATTTGGCAATAGTGCTGTCCTGTGTTAG

Protein sequence

>Msa0695100

MMRKSMSLRGLNENRTINSEQMTGVAETSKSIQLKTHKVLSSSLYIPSFQHSFGNSAVLC*