AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1114590


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114590 MtrunA17_Chr7g0257271 100.000 313 0 0 1 313 1 313 0.0 649
Msa1114590 MtrunA17_Chr3g0091551 92.652 313 23 0 1 313 1 313 0.0 608
Msa1114590 MtrunA17_Chr3g0092431 81.935 310 51 1 1 310 1 305 0.0 528
Msa1114590 MtrunA17_Chr3g0092491 74.760 313 36 1 1 313 1 270 2.82e-164 457
Msa1114590 MtrunA17_Chr1g0173141 81.865 193 34 1 121 313 4 195 5.61e-115 330
Msa1114590 MtrunA17_Chr1g0173151 79.661 118 24 0 1 118 1 118 8.19e-64 197
Msa1114590 MtrunA17_Chr1g0156011 67.416 89 22 2 210 291 28 116 1.36e-29 109
Msa1114590 MtrunA17_Chr5g0403861 34.184 196 121 4 108 301 134 323 1.55e-21 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1114590 AT3G10330.1 92.652 313 22 1 1 313 1 312 0.0 603
Msa1114590 AT2G41630.1 84.984 313 46 1 1 313 1 312 0.0 558
Msa1114590 AT3G29380.1 42.767 318 171 5 1 307 1 318 1.72e-83 255
Msa1114590 AT3G57370.1 33.333 369 170 10 6 313 9 362 2.97e-47 162
Msa1114590 AT4G10680.1 34.694 196 102 5 3 195 4 176 2.16e-23 95.1
Msa1114590 AT4G36650.1 34.694 196 120 4 108 301 133 322 8.35e-22 95.9
Msa1114590 AT4G36650.2 35.057 174 105 4 108 279 133 300 6.90e-18 83.6

Find 117 sgRNAs with CRISPR-Local

Find 2 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTCATGCTGGGGACTTTA+TGG 0.146027 7_3:-61439497 Msa1114590:intron
TGCGCGGCCAATTTCCTTCT+TGG 0.168298 7_3:+61439582 None:intergenic
GCCTCCTCATTCAAATATTT+AGG 0.192290 7_3:-61437310 Msa1114590:three_prime_UTR
CCTCCTCATTCAAATATTTA+GGG 0.259960 7_3:-61437309 Msa1114590:three_prime_UTR
AACTGCTGCAGCAATTGATA+TGG 0.274816 7_3:+61438468 None:intergenic
ACGAAATACACATACCTTAA+TGG 0.290265 7_3:+61441090 None:intergenic
ATCCTTGCGTTCAAAACTAT+TGG 0.312072 7_3:-61441339 Msa1114590:CDS
ATCGGTAAGGAGAGGGTTAC+TGG 0.314300 7_3:+61441470 None:intergenic
GAAAGTTAAAATTGTTGTAT+TGG 0.321478 7_3:-61437408 Msa1114590:three_prime_UTR
AAGAAATCAGGACGCATTAT+TGG 0.327460 7_3:-61439779 Msa1114590:CDS
ATCATAGGATGTACCCTTTA+CGG 0.330716 7_3:+61439711 None:intergenic
GGGACTGGTTCCGACCATTA+AGG 0.332605 7_3:-61441104 Msa1114590:intron
GTTCAAAACTATTGGTACTA+TGG 0.338830 7_3:-61441331 Msa1114590:CDS
TTCCTTCTTGGTGGCTCCAT+TGG 0.342202 7_3:+61439594 None:intergenic
TGAAACGGAGGATAAATTAA+TGG 0.342541 7_3:+61441838 None:intergenic
TGGCGGTCAATCTGTTGAAA+TGG 0.354243 7_3:-61439524 Msa1114590:CDS
AATCAGAAGAATTTGATATA+AGG 0.356784 7_3:-61439278 Msa1114590:intron
GGAGCCACCAAGAAGGAAAT+TGG 0.363081 7_3:-61439589 Msa1114590:CDS
TTTAACTTTCAATGACATCT+AGG 0.392497 7_3:+61437421 None:intergenic
GTCTAGCAGGGGAAGAAATC+AGG 0.396384 7_3:-61439791 Msa1114590:CDS
CATAGTGAAACAACTGGGTT+TGG 0.405085 7_3:-61439551 Msa1114590:CDS
TAAAGCTGATGAAAATCATT+GGG 0.416989 7_3:-61437209 Msa1114590:three_prime_UTR
GACAAGCCACGCACCGTAAA+GGG 0.419868 7_3:-61439724 Msa1114590:intron
TCTTCCTTCGCATACCAGTT+TGG 0.420592 7_3:+61437501 None:intergenic
AGAATACATAGTGAAACAAC+TGG 0.423190 7_3:-61439557 Msa1114590:CDS
GTTTCCGAGTTTCTCTCTCT+AGG 0.426134 7_3:+61441678 None:intergenic
TTGATTGAAGATGTGTTTGA+TGG 0.427255 7_3:+61441866 None:intergenic
CCCTTGAAACAAAACAGTTC+CGG 0.430987 7_3:-61437457 Msa1114590:exon
CGAACCTTCGCTAACGAATC+CGG 0.431290 7_3:-61441522 Msa1114590:CDS
CTGGGACCACCGACACGAAC+AGG 0.432295 7_3:+61441489 None:intergenic
TTCGGAGTGTGGTCTCGTCT+TGG 0.434035 7_3:-61441577 Msa1114590:CDS
TTCAAATATTTAGGGGAGAC+TGG 0.434287 7_3:-61437301 Msa1114590:three_prime_UTR
TTTCAAAGATAATACCAAAC+TGG 0.453182 7_3:-61437515 Msa1114590:CDS
CGCCGGCGATGCAGTCTGTT+CGG 0.458749 7_3:-61441595 Msa1114590:CDS
ATCCTTGTATGAGTTCCTAA+TGG 0.459175 7_3:+61437551 None:intergenic
GGCGGTCAATCTGTTGAAAT+GGG 0.469059 7_3:-61439523 Msa1114590:CDS
AAACAACTGGGTTTGGAGAA+TGG 0.469627 7_3:-61439544 Msa1114590:CDS
TCAAGCTGTTAAAGCTGCTC+AGG 0.470264 7_3:-61439313 Msa1114590:CDS
TCTCGTCTTCTCTCGGTCGT+TGG 0.473284 7_3:-61441395 Msa1114590:CDS
GTAAAGCTGATGAAAATCAT+TGG 0.475566 7_3:-61437210 Msa1114590:three_prime_UTR
AACGATCCTGTTCGTGTCGG+TGG 0.478254 7_3:-61441495 Msa1114590:CDS
AAACACAATTAACACATCAA+TGG 0.482079 7_3:-61441782 Msa1114590:five_prime_UTR
CTCCGAACAGACTGCATCGC+CGG 0.488164 7_3:+61441593 None:intergenic
GCGGCCAATTTCCTTCTTGG+TGG 0.490289 7_3:+61439585 None:intergenic
TGAGAGCGTTTGAAATGAAA+CGG 0.497457 7_3:+61441823 None:intergenic
ACTGCTGCAGCAATTGATAT+GGG 0.502657 7_3:+61438469 None:intergenic
AAATCACCGGACGCTCCATT+CGG 0.505266 7_3:+61441420 None:intergenic
AGACAAGCCACGCACCGTAA+AGG 0.506939 7_3:-61439725 Msa1114590:CDS
TTTCCGAGTTTCTCTCTCTA+GGG 0.508097 7_3:+61441679 None:intergenic
TTGAAGATCAGAAGTCTAGC+AGG 0.514009 7_3:-61439804 Msa1114590:CDS
TGGTATTATCTTTGAAACAT+GGG 0.517194 7_3:+61437521 None:intergenic
CGACTGCAAGCGAGCGACGG+AGG 0.519185 7_3:-61441634 Msa1114590:CDS
GCAGATATATCAGTTGCTAC+AGG 0.531635 7_3:-61437588 Msa1114590:intron
CAGCGACTGCAAGCGAGCGA+CGG 0.532018 7_3:-61441637 Msa1114590:CDS
GAAATGGGAACAATTCATGC+TGG 0.537995 7_3:-61439508 Msa1114590:CDS
CGCAAGGATCAATCCACGAT+CGG 0.538169 7_3:+61441353 None:intergenic
CTGCAAGCGAGCGACGGAGG+TGG 0.539765 7_3:-61441631 Msa1114590:CDS
GCAAGGATCAATCCACGATC+GGG 0.539890 7_3:+61441354 None:intergenic
GAAATTTGCTCGATTGCCAA+TGG 0.542448 7_3:-61439610 Msa1114590:CDS
AAATGGGAACAATTCATGCT+GGG 0.542849 7_3:-61439507 Msa1114590:CDS
TCTAGGTTGTTTAATATCAC+CGG 0.543333 7_3:+61437438 None:intergenic
CCACTCGGAGGTTTCATCGA+TGG 0.547579 7_3:+61441545 None:intergenic
GTTAGCGAAGGTTCGCCACT+CGG 0.548954 7_3:+61441530 None:intergenic
TTGGTATTATCTTTGAAACA+TGG 0.552826 7_3:+61437520 None:intergenic
AGCTAATGAGATATATAAGC+GGG 0.555073 7_3:-61439827 Msa1114590:CDS
GCTAAGCCGAATGGAGCGTC+CGG 0.558353 7_3:-61441426 Msa1114590:CDS
ACTGTCATTGCTAAGCCGAA+TGG 0.565002 7_3:-61441435 Msa1114590:CDS
AACCATTAGGAACTCATACA+AGG 0.569754 7_3:-61437553 Msa1114590:CDS
TGGAGAGACCACCATCGGTA+AGG 0.571293 7_3:+61441457 None:intergenic
GCTGCAAAACGCATCGGACA+TGG 0.572012 7_3:+61441656 None:intergenic
CCCTAAATATTTGAATGAGG+AGG 0.572120 7_3:+61437309 None:intergenic
GGTATTATCTTTGAAACATG+GGG 0.573323 7_3:+61437522 None:intergenic
CTCCCCTAAATATTTGAATG+AGG 0.575253 7_3:+61437306 None:intergenic
AACCCTCTCCTTACCGATGG+TGG 0.575511 7_3:-61441465 Msa1114590:CDS
GAGTTGCAGAAGGAACCATT+AGG 0.581627 7_3:-61437566 Msa1114590:CDS
GATGTACCCTTTACGGTGCG+TGG 0.585661 7_3:+61439718 None:intergenic
TGCCAATGGAGCCACCAAGA+AGG 0.588006 7_3:-61439596 Msa1114590:CDS
AATACACATACCTTAATGGT+CGG 0.591568 7_3:+61441094 None:intergenic
TCGGTAAGGAGAGGGTTACT+GGG 0.602364 7_3:+61441471 None:intergenic
CTCCTCATTCAAATATTTAG+GGG 0.603075 7_3:-61437308 Msa1114590:three_prime_UTR
GACCACCATCGGTAAGGAGA+GGG 0.603996 7_3:+61441463 None:intergenic
GATGCAGTCTGTTCGGAGTG+TGG 0.606400 7_3:-61441588 Msa1114590:CDS
GCAGTCGCTGCAAAACGCAT+CGG 0.606677 7_3:+61441650 None:intergenic
CTCGGTCGTTGGCAGAACCG+TGG 0.609956 7_3:-61441384 Msa1114590:CDS
ATCGCCGGATTCGTTAGCGA+AGG 0.611270 7_3:+61441518 None:intergenic
GCTTATATATCTCATTAGCT+CGG 0.615889 7_3:+61439830 None:intergenic
AGAAGACGAGAGAAAATCAC+CGG 0.618673 7_3:+61441407 None:intergenic
AGTAACCCTCTCCTTACCGA+TGG 0.620939 7_3:-61441468 Msa1114590:CDS
AATACCAAACTGGTATGCGA+AGG 0.621663 7_3:-61437505 Msa1114590:CDS
TGAAGATCAGAAGTCTAGCA+GGG 0.622654 7_3:-61439803 Msa1114590:CDS
GTGGTGTTCGACCACTCCGC+CGG 0.623423 7_3:-61441612 Msa1114590:CDS
AGCGAAGGTTCGCCACTCGG+AGG 0.626549 7_3:+61441533 None:intergenic
AGACTGCATCGCCGGCGGAG+TGG 0.627397 7_3:+61441601 None:intergenic
AACTGTCACAGACCTTTGAG+AGG 0.627588 7_3:+61438409 None:intergenic
AAACCCTAGAGAGAGAAACT+CGG 0.633229 7_3:-61441682 Msa1114590:five_prime_UTR
AAACTGGTATGCGAAGGAAG+AGG 0.641876 7_3:-61437499 Msa1114590:CDS
CTTGTCGACAAGCAATGTAG+AGG 0.643119 7_3:+61439749 None:intergenic
GTTGCTACAGGAGTTGCAGA+AGG 0.648786 7_3:-61437576 Msa1114590:CDS
GACAGTGGAGAGACCACCAT+CGG 0.651740 7_3:+61441452 None:intergenic
CGTGGTTCTAATCCCGATCG+TGG 0.653015 7_3:-61441366 Msa1114590:CDS
TTAAATGAAATGGACAACAA+AGG 0.653926 7_3:+61437183 None:intergenic
CGAACAGACTGCATCGCCGG+CGG 0.655902 7_3:+61441596 None:intergenic
ACGAACAGGATCGTTATCGC+CGG 0.656747 7_3:+61441503 None:intergenic
CTATTGGTACTATGGCTGAG+AGG 0.657231 7_3:-61441323 Msa1114590:intron
AGACCACCATCGGTAAGGAG+AGG 0.659672 7_3:+61441462 None:intergenic
GAAGATCAGAAGTCTAGCAG+GGG 0.664166 7_3:-61439802 Msa1114590:CDS
AATGGGAACAATTCATGCTG+GGG 0.664649 7_3:-61439506 Msa1114590:CDS
GATAACGATCCTGTTCGTGT+CGG 0.671320 7_3:-61441498 Msa1114590:CDS
CACGATCGGGATTAGAACCA+CGG 0.671797 7_3:+61441367 None:intergenic
ATATTTAGGGGAGACTGGAG+AGG 0.678070 7_3:-61437296 Msa1114590:three_prime_UTR
GAGCTAATGAGATATATAAG+CGG 0.694381 7_3:-61439828 Msa1114590:CDS
AAAGCTGATGAAAATCATTG+GGG 0.698878 7_3:-61437208 Msa1114590:three_prime_UTR
ATTCGGCTTAGCAATGACAG+TGG 0.701850 7_3:+61441437 None:intergenic
GAATACATAGTGAAACAACT+GGG 0.707930 7_3:-61439556 Msa1114590:CDS
GAGCGTTTGAAATGAAACGG+AGG 0.735680 7_3:+61441826 None:intergenic
CCATCGATGAAACCTCCGAG+TGG 0.757869 7_3:-61441545 Msa1114590:CDS
CAACTGGGTTTGGAGAATGG+CGG 0.759270 7_3:-61439541 Msa1114590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TGAAACGGAGGATAAATTAA+TGG + chr7_3:61437222-61437241 None:intergenic 30.0%
! GAGCGTTTGAAATGAAACGG+AGG + chr7_3:61437234-61437253 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 61437193 61441886 61437193 ID=Msa1114590;Name=Msa1114590
chr7_3 mRNA 61437193 61441886 61437193 ID=Msa1114590-mRNA-1;Parent=Msa1114590;Name=Msa1114590-mRNA-1;_AED=0.06;_eAED=0.06;_QI=210|1|1|1|1|1|7|280|313
chr7_3 exon 61437193 61437606 61437193 ID=Msa1114590-mRNA-1:exon:13984;Parent=Msa1114590-mRNA-1
chr7_3 exon 61438422 61438498 61438422 ID=Msa1114590-mRNA-1:exon:13983;Parent=Msa1114590-mRNA-1
chr7_3 exon 61439279 61439367 61439279 ID=Msa1114590-mRNA-1:exon:13982;Parent=Msa1114590-mRNA-1
chr7_3 exon 61439498 61439631 61439498 ID=Msa1114590-mRNA-1:exon:13981;Parent=Msa1114590-mRNA-1
chr7_3 exon 61439725 61439854 61439725 ID=Msa1114590-mRNA-1:exon:13980;Parent=Msa1114590-mRNA-1
chr7_3 exon 61441105 61441129 61441105 ID=Msa1114590-mRNA-1:exon:13979;Parent=Msa1114590-mRNA-1
chr7_3 exon 61441324 61441886 61441324 ID=Msa1114590-mRNA-1:exon:13978;Parent=Msa1114590-mRNA-1
chr7_3 five_prime_UTR 61441677 61441886 61441677 ID=Msa1114590-mRNA-1:five_prime_utr;Parent=Msa1114590-mRNA-1
chr7_3 CDS 61441324 61441676 61441324 ID=Msa1114590-mRNA-1:cds;Parent=Msa1114590-mRNA-1
chr7_3 CDS 61441105 61441129 61441105 ID=Msa1114590-mRNA-1:cds;Parent=Msa1114590-mRNA-1
chr7_3 CDS 61439725 61439854 61439725 ID=Msa1114590-mRNA-1:cds;Parent=Msa1114590-mRNA-1
chr7_3 CDS 61439498 61439631 61439498 ID=Msa1114590-mRNA-1:cds;Parent=Msa1114590-mRNA-1
chr7_3 CDS 61439279 61439367 61439279 ID=Msa1114590-mRNA-1:cds;Parent=Msa1114590-mRNA-1
chr7_3 CDS 61438422 61438498 61438422 ID=Msa1114590-mRNA-1:cds;Parent=Msa1114590-mRNA-1
chr7_3 CDS 61437473 61437606 61437473 ID=Msa1114590-mRNA-1:cds;Parent=Msa1114590-mRNA-1
chr7_3 three_prime_UTR 61437193 61437472 61437193 ID=Msa1114590-mRNA-1:three_prime_utr;Parent=Msa1114590-mRNA-1
Gene Sequence

>Msa1114590

ATGTCCGATGCGTTTTGCAGCGACTGCAAGCGAGCGACGGAGGTGGTGTTCGACCACTCCGCCGGCGATGCAGTCTGTTCGGAGTGTGGTCTCGTCTTGGAATCTCACTCCATCGATGAAACCTCCGAGTGGCGAACCTTCGCTAACGAATCCGGCGATAACGATCCTGTTCGTGTCGGTGGTCCCAGTAACCCTCTCCTTACCGATGGTGGTCTCTCCACTGTCATTGCTAAGCCGAATGGAGCGTCCGGTGATTTTCTCTCGTCTTCTCTCGGTCGTTGGCAGAACCGTGGTTCTAATCCCGATCGTGGATTGATCCTTGCGTTCAAAACTATTGGTACTATGGCTGAGAGGTTGGGACTGGTTCCGACCATTAAGGACCGAGCTAATGAGATATATAAGCGGGTTGAAGATCAGAAGTCTAGCAGGGGAAGAAATCAGGACGCATTATTGGCTGCTTGCCTCTACATTGCTTGTCGACAAGAAGACAAGCCACGCACCGTAAAGGAAATTTGCTCGATTGCCAATGGAGCCACCAAGAAGGAAATTGGCCGCGCAAAAGAATACATAGTGAAACAACTGGGTTTGGAGAATGGCGGTCAATCTGTTGAAATGGGAACAATTCATGCTGGGGACTTTATGAGACGATTTTGTTCTAATCTTGGTATGAATCATCAAGCTGTTAAAGCTGCTCAGGAATCTGTTCAGAAATCAGAAGAATTTGATATAAGGAGAAGTCCCATATCAATTGCTGCAGCAGTTATATACATCATTACTCAGCTTTCAGATGATAAAAAACCTCTCAAAGATATATCAGTTGCTACAGGAGTTGCAGAAGGAACCATTAGGAACTCATACAAGGATCTCTATCCCCATGTTTCAAAGATAATACCAAACTGGTATGCGAAGGAAGAGGATCTGAAGAACCTTTGCAGCCCTTGA

Protein sequence

>Msa1114590

MSDAFCSDCKRATEVVFDHSAGDAVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPSNPLLTDGGLSTVIAKPNGASGDFLSSSLGRWQNRGSNPDRGLILAFKTIGTMAERLGLVPTIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSIANGATKKEIGRAKEYIVKQLGLENGGQSVEMGTIHAGDFMRRFCSNLGMNHQAVKAAQESVQKSEEFDIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPNWYAKEEDLKNLCSP*