AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1084800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1084800 MtrunA17_Chr7g0252501 83.721 86 14 0 1 86 24 109 5.06e-49 154
Msa1084800 MtrunA17_Chr3g0107871 73.256 86 21 1 3 86 139 224 1.11e-38 131
Msa1084800 MtrunA17_Chr7g0252361 55.294 85 37 1 3 86 160 244 6.08e-24 92.8
Msa1084800 MtrunA17_Chr6g0455071 48.052 77 38 1 12 86 45 121 2.33e-20 79.0
Msa1084800 MtrunA17_Chr3g0144871 50.000 74 35 1 12 83 171 244 1.84e-18 78.2
Msa1084800 MtrunA17_Chr8g0372671 46.512 86 40 1 11 90 164 249 1.29e-17 75.9
Msa1084800 MtrunA17_Chr4g0008111 50.000 76 32 1 15 84 168 243 1.71e-17 75.5
Msa1084800 MtrunA17_Chr6g0475241 48.193 83 37 1 10 86 164 246 3.25e-17 74.7
Msa1084800 MtrunA17_Chr6g0475221 45.882 85 39 2 4 86 158 237 8.23e-17 73.6
Msa1084800 MtrunA17_Chr1g0155481 39.796 98 52 2 2 92 8 105 1.17e-16 70.1
Msa1084800 MtrunA17_Chr1g0155451 38.542 96 47 2 3 86 9 104 6.50e-16 69.3
Msa1084800 MtrunA17_Chr5g0404631 43.333 90 40 2 3 86 172 256 2.39e-15 69.3
Msa1084800 MtrunA17_Chr8g0372821 44.737 76 36 1 15 84 168 243 1.99e-14 67.0
Msa1084800 MtrunA17_Chr3g0107591 43.750 80 39 1 11 84 163 242 6.80e-14 65.5
Msa1084800 MtrunA17_Chr8g0343971 42.353 85 42 1 15 92 168 252 9.52e-14 65.1
Msa1084800 MtrunA17_Chr5g0419851 42.857 77 38 1 14 90 233 303 4.51e-13 63.2
Msa1084800 MtrunA17_Chr8g0343981 43.529 85 41 1 15 92 168 252 5.32e-13 62.8
Msa1084800 MtrunA17_Chr8g0372801 41.791 67 39 0 10 76 104 170 1.01e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1084800 AT5G66430.1 49.231 65 28 1 3 67 149 208 1.76e-15 70.1
Msa1084800 AT3G21950.2 39.130 92 45 2 3 88 156 242 2.21e-14 67.0
Msa1084800 AT5G04370.3 48.438 64 28 1 4 67 196 254 2.98e-14 66.6
Msa1084800 AT5G04370.1 47.692 65 29 1 3 67 190 249 5.54e-14 65.9
Msa1084800 AT3G11480.1 47.761 67 33 1 3 67 174 240 5.96e-14 65.9
Msa1084800 AT5G04370.2 47.692 65 29 1 3 67 209 268 6.81e-14 65.9
Msa1084800 AT3G21950.1 47.692 65 29 1 3 67 156 215 6.97e-14 65.9
Msa1084800 AT5G38020.2 50.943 53 26 0 15 67 124 176 2.94e-13 63.9
Msa1084800 AT5G38020.1 46.154 65 30 1 3 67 156 215 4.62e-13 63.5
Msa1084800 AT5G04380.2 45.312 64 30 1 4 67 116 174 2.76e-12 61.2
Msa1084800 AT5G04380.1 45.312 64 30 1 4 67 183 241 3.15e-12 61.2
Msa1084800 AT5G04380.3 45.312 64 30 1 4 67 187 245 4.66e-12 60.5
Msa1084800 AT1G19640.1 45.763 59 32 0 15 73 186 244 2.16e-11 58.5

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCTTGGCCAAACCTTTC+GGG 0.134603 7_2:+85185239 None:intergenic
GTTTAGTGGTTCTCCATTCT+TGG 0.202312 7_2:+85185225 None:intergenic
CCATTCTTGGCCAAACCTTT+CGG 0.227156 7_2:+85185238 None:intergenic
TAAATATTTCCCTTGTTTAG+TGG 0.357448 7_2:+85185211 None:intergenic
CAAATCTCATCATTACCTCT+TGG 0.415227 7_2:+85184996 None:intergenic
CATTTAGGCCCCGAAAGGTT+TGG 0.417248 7_2:-85185248 Msa1084800:intron
TTAAAATATGCTTCATAGAC+TGG 0.421359 7_2:+85185166 None:intergenic
CCGAAAGGTTTGGCCAAGAA+TGG 0.461973 7_2:-85185238 Msa1084800:CDS
TTCGAGGAATTAACAACTGA+TGG 0.468183 7_2:-85185103 Msa1084800:CDS
ATGATGGCATTAACATTTGT+TGG 0.472815 7_2:-85185079 Msa1084800:CDS
ATTAACAACTGATGGGATGA+TGG 0.518595 7_2:-85185095 Msa1084800:CDS
TTCTCAAGGGGTAATTGCCA+TGG 0.534080 7_2:-85185035 Msa1084800:CDS
TATGCTTCATAGACTGGTGG+AGG 0.535027 7_2:+85185172 None:intergenic
ATTCTTGGCCAAACCTTTCG+GGG 0.560520 7_2:+85185240 None:intergenic
AACTACCACTTCTTCTCAAG+GGG 0.577750 7_2:-85185047 Msa1084800:CDS
TGCCATGGTACTCAATGAGA+TGG 0.578688 7_2:-85185020 Msa1084800:CDS
ACAACTACCACTTCTTCTCA+AGG 0.579517 7_2:-85185049 Msa1084800:CDS
CAACTACCACTTCTTCTCAA+GGG 0.581594 7_2:-85185048 Msa1084800:CDS
TCGAGGAATTAACAACTGAT+GGG 0.582747 7_2:-85185102 Msa1084800:CDS
GAATGGAGAACCACTAAACA+AGG 0.590677 7_2:-85185221 Msa1084800:CDS
AATTACCCCTTGAGAAGAAG+TGG 0.590678 7_2:+85185042 None:intergenic
AAATATGCTTCATAGACTGG+TGG 0.602942 7_2:+85185169 None:intergenic
TGTGACATTTAGGCCCCGAA+AGG 0.611420 7_2:-85185253 Msa1084800:intron
ATGCTTCATAGACTGGTGGA+GGG 0.673189 7_2:+85185173 None:intergenic
GACCATCTCATTGAGTACCA+TGG 0.681630 7_2:+85185018 None:intergenic
ACTCAATGAGATGGTCCAAG+AGG 0.686877 7_2:-85185011 Msa1084800:CDS
AATGGAGAACCACTAAACAA+GGG 0.694091 7_2:-85185220 Msa1084800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTTCTTTGTGACATTT+AGG - chr7_2:85185175-85185194 Msa1084800:CDS 20.0%
!! TAAATATTTCCCTTGTTTAG+TGG + chr7_2:85185230-85185249 None:intergenic 25.0%
! TTAAAATATGCTTCATAGAC+TGG + chr7_2:85185275-85185294 None:intergenic 25.0%
ATGATGGCATTAACATTTGT+TGG - chr7_2:85185359-85185378 Msa1084800:intron 30.0%
AATGGAGAACCACTAAACAA+GGG - chr7_2:85185218-85185237 Msa1084800:CDS 35.0%
AAATATGCTTCATAGACTGG+TGG + chr7_2:85185272-85185291 None:intergenic 35.0%
!!! TTTTCTAAAGTCACGTTTCG+AGG - chr7_2:85185319-85185338 Msa1084800:intron 35.0%
TTCGAGGAATTAACAACTGA+TGG - chr7_2:85185335-85185354 Msa1084800:intron 35.0%
TCGAGGAATTAACAACTGAT+GGG - chr7_2:85185336-85185355 Msa1084800:intron 35.0%
ATTAACAACTGATGGGATGA+TGG - chr7_2:85185343-85185362 Msa1084800:intron 35.0%
CAAATCTCATCATTACCTCT+TGG + chr7_2:85185445-85185464 None:intergenic 35.0%
GTTTAGTGGTTCTCCATTCT+TGG + chr7_2:85185216-85185235 None:intergenic 40.0%
GAATGGAGAACCACTAAACA+AGG - chr7_2:85185217-85185236 Msa1084800:CDS 40.0%
ACAACTACCACTTCTTCTCA+AGG - chr7_2:85185389-85185408 Msa1084800:intron 40.0%
CAACTACCACTTCTTCTCAA+GGG - chr7_2:85185390-85185409 Msa1084800:intron 40.0%
AACTACCACTTCTTCTCAAG+GGG - chr7_2:85185391-85185410 Msa1084800:intron 40.0%
AATTACCCCTTGAGAAGAAG+TGG + chr7_2:85185399-85185418 None:intergenic 40.0%
ATTCTTGGCCAAACCTTTCG+GGG + chr7_2:85185201-85185220 None:intergenic 45.0%
CATTCTTGGCCAAACCTTTC+GGG + chr7_2:85185202-85185221 None:intergenic 45.0%
CCATTCTTGGCCAAACCTTT+CGG + chr7_2:85185203-85185222 None:intergenic 45.0%
ATGCTTCATAGACTGGTGGA+GGG + chr7_2:85185268-85185287 None:intergenic 45.0%
TATGCTTCATAGACTGGTGG+AGG + chr7_2:85185269-85185288 None:intergenic 45.0%
TTCTCAAGGGGTAATTGCCA+TGG - chr7_2:85185403-85185422 Msa1084800:intron 45.0%
TGCCATGGTACTCAATGAGA+TGG - chr7_2:85185418-85185437 Msa1084800:intron 45.0%
GACCATCTCATTGAGTACCA+TGG + chr7_2:85185423-85185442 None:intergenic 45.0%
ACTCAATGAGATGGTCCAAG+AGG - chr7_2:85185427-85185446 Msa1084800:intron 45.0%
TGTCAGCAACATAGCAGAGC+TGG - chr7_2:85185011-85185030 Msa1084800:CDS 50.0%
GTCAGCAACATAGCAGAGCT+GGG - chr7_2:85185012-85185031 Msa1084800:CDS 50.0%
TGTGACATTTAGGCCCCGAA+AGG - chr7_2:85185185-85185204 Msa1084800:CDS 50.0%
CATTTAGGCCCCGAAAGGTT+TGG - chr7_2:85185190-85185209 Msa1084800:CDS 50.0%
CCGAAAGGTTTGGCCAAGAA+TGG - chr7_2:85185200-85185219 Msa1084800:CDS 50.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 85184985 85185475 85184985 ID=Msa1084800;Name=Msa1084800
chr7_2 mRNA 85184985 85185475 85184985 ID=Msa1084800-mRNA-1;Parent=Msa1084800;Name=Msa1084800-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0|0|0|0.5|1|1|2|0|94
chr7_2 exon 85185470 85185475 85185470 ID=Msa1084800-mRNA-1:exon:23022;Parent=Msa1084800-mRNA-1
chr7_2 exon 85184985 85185263 85184985 ID=Msa1084800-mRNA-1:exon:23021;Parent=Msa1084800-mRNA-1
chr7_2 CDS 85185470 85185475 85185470 ID=Msa1084800-mRNA-1:cds;Parent=Msa1084800-mRNA-1
chr7_2 CDS 85184985 85185263 85184985 ID=Msa1084800-mRNA-1:cds;Parent=Msa1084800-mRNA-1
Gene Sequence

>Msa1084800

ATGAATGCCCCGAAAGGTTTGGCCAAGAATGGAGAACCACTAAACAAGGGAAATATTTACATATCACGTACATGCCCTCCACCAGTCTATGAAGCATATTTTAAGCAATTTGAAAGAGATTTCAAGTCTTTTCTAAAGTCACGTTTCGAGGAATTAACAACTGATGGGATGATGGCATTAACATTTGTTGGCAGAGAAACAACTACCACTTCTTCTCAAGGGGTAATTGCCATGGTACTCAATGAGATGGTCCAAGAGGTAATGATGAGATTTGATAATAAATGA

Protein sequence

>Msa1084800

MNAPKGLAKNGEPLNKGNIYISRTCPPPVYEAYFKQFERDFKSFLKSRFEELTTDGMMALTFVGRETTTTSSQGVIAMVLNEMVQEVMMRFDNK*