AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07440.t1 MTR_1g076920 98.788 165 2 0 1 165 226 390 8.12e-117 336
MS.gene07440.t1 MTR_5g056200 33.813 139 87 2 17 155 197 330 6.21e-24 95.9
MS.gene07440.t1 MTR_8g077185 36.620 142 80 4 28 165 230 365 4.13e-17 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07440.t1 AT5G38520 73.333 165 44 0 1 165 207 371 7.78e-79 239
MS.gene07440.t1 AT5G38520 73.333 165 44 0 1 165 195 359 8.77e-79 238
MS.gene07440.t1 AT5G19850 32.374 139 89 2 17 155 216 349 6.46e-21 87.8
MS.gene07440.t1 AT4G36530 34.043 141 81 4 28 163 173 306 1.67e-15 72.8
MS.gene07440.t1 AT4G36530 34.043 141 81 4 28 163 230 363 3.35e-15 72.0

Find 43 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCCCATAAAAGTAAAAT+AGG 0.274171 1.2:-63546125 None:intergenic
GCATTTGTATCAATTGTAAC+AGG 0.286676 1.2:+63546056 MS.gene07440:CDS
AATTGATACAAATGCATCTA+AGG 0.330008 1.2:-63546048 None:intergenic
TTGCAGAAGCAATTCCCTTT+TGG 0.333173 1.2:-63545644 None:intergenic
GAGTTCAGTGTATGGAAATA+AGG 0.338288 1.2:+63545786 MS.gene07440:CDS
ACAAAGCAATTGTTGATGAT+TGG 0.341853 1.2:+63545571 MS.gene07440:CDS
AGTTACTCCTTCCCTTGCTT+TGG 0.349148 1.2:+63545601 MS.gene07440:CDS
GATCCCTTTACTCCAATTGA+TGG 0.363586 1.2:+63546152 MS.gene07440:CDS
GGCACCAGTTGCACCGGATT+AGG 0.382985 1.2:-63546089 None:intergenic
TTTGGTTCCTCAGATCATTA+GGG 0.394374 1.2:+63546004 MS.gene07440:intron
GGTCCATCAATTGGAGTAAA+GGG 0.415428 1.2:-63546155 None:intergenic
GTTCAACTGTTCATGCTTGA+AGG 0.416044 1.2:+63546221 MS.gene07440:CDS
AGGGCCGCCGGGGCCTAATC+CGG 0.428980 1.2:+63546076 MS.gene07440:CDS
TCCTCAGATCATTAGGGAAC+CGG 0.429301 1.2:+63546010 MS.gene07440:intron
TTTCCAACAGGTCCATCAAT+TGG 0.431388 1.2:-63546164 None:intergenic
GGGGCCTAATCCGGTGCAAC+TGG 0.436807 1.2:+63546085 MS.gene07440:CDS
TTAAATGCAGAGAGAATTTG+AGG 0.438567 1.2:+63545755 MS.gene07440:intron
GAAGAAAAGTACTTTCCAAC+AGG 0.449114 1.2:-63546176 None:intergenic
AGGTCCATCAATTGGAGTAA+AGG 0.483632 1.2:-63546156 None:intergenic
GACATTGTCCCCATGATGAC+AGG 0.489475 1.2:+63546249 MS.gene07440:CDS
TCAATTGTAACAGGGCCGCC+GGG 0.490938 1.2:+63546065 MS.gene07440:CDS
ACTCCAATTGATGGACCTGT+TGG 0.491390 1.2:+63546161 MS.gene07440:CDS
TTCAACTGTTCATGCTTGAA+GGG 0.491418 1.2:+63546222 MS.gene07440:CDS
ATCAATTGTAACAGGGCCGC+CGG 0.498322 1.2:+63546064 MS.gene07440:CDS
CTAATTCAGGCCTGTCATCA+TGG 0.500280 1.2:-63546259 None:intergenic
CTGTTCATGCTTGAAGGGGT+TGG 0.503540 1.2:+63546227 MS.gene07440:CDS
AACATCTTGAGTTCAGTGTA+TGG 0.513396 1.2:+63545778 MS.gene07440:CDS
TAATTCAGGCCTGTCATCAT+GGG 0.516079 1.2:-63546258 None:intergenic
AAAATCAATCAGCCAAAGCA+AGG 0.543664 1.2:-63545613 None:intergenic
AAGGCTCCTTCAGCATTCGC+CGG 0.569196 1.2:-63546029 None:intergenic
AGGGAACCGGCGAATGCTGA+AGG 0.582575 1.2:+63546023 MS.gene07440:CDS
GTATGGAAATAAGGAGGCTG+TGG 0.584738 1.2:+63545795 MS.gene07440:CDS
AAATCAATCAGCCAAAGCAA+GGG 0.595342 1.2:-63545612 None:intergenic
AGTTGCACCGGATTAGGCCC+CGG 0.614113 1.2:-63546083 None:intergenic
CAATCAGCCAAAGCAAGGGA+AGG 0.620564 1.2:-63545608 None:intergenic
CATTTGTATCAATTGTAACA+GGG 0.626940 1.2:+63546057 MS.gene07440:CDS
GGCTGTGGATGATGAACTCG+TGG 0.631002 1.2:+63545810 MS.gene07440:CDS
TTCAGTGTATGGAAATAAGG+AGG 0.648155 1.2:+63545789 MS.gene07440:CDS
CAATTGTAACAGGGCCGCCG+GGG 0.653302 1.2:+63546066 MS.gene07440:CDS
TCAACTGTTCATGCTTGAAG+GGG 0.658262 1.2:+63546223 MS.gene07440:CDS
AATTCAGGCCTGTCATCATG+GGG 0.687756 1.2:-63546257 None:intergenic
TGTGGATGATGAACTCGTGG+AGG 0.755163 1.2:+63545813 MS.gene07440:CDS
TGCACCGGATTAGGCCCCGG+CGG 0.755765 1.2:-63546080 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTATAAGAATTCATATTT+TGG + chr1.2:63545986-63546005 MS.gene07440:intron 15.0%
!! AAAAAAATTAATACTCCTTC+CGG - chr1.2:63545949-63545968 None:intergenic 20.0%
!! CAAAAAATTTAGGGAAATTA+TGG - chr1.2:63545711-63545730 None:intergenic 20.0%
!! GTTTCCTATCAAAAAATTTA+GGG - chr1.2:63545720-63545739 None:intergenic 20.0%
!! TGTTTCCTATCAAAAAATTT+AGG - chr1.2:63545721-63545740 None:intergenic 20.0%
!!! AAAATAGGCAATGATATTTT+TGG - chr1.2:63546113-63546132 None:intergenic 20.0%
!!! ATTGCCTATTTTACTTTTAT+GGG + chr1.2:63546121-63546140 MS.gene07440:CDS 20.0%
!!! ATTTCCCTAAATTTTTTGAT+AGG + chr1.2:63545713-63545732 MS.gene07440:intron 20.0%
!!! GATTTTTTATTGAACCAAAA+GGG + chr1.2:63545630-63545649 MS.gene07440:CDS 20.0%
!!! TGATTTTTTATTGAACCAAA+AGG + chr1.2:63545629-63545648 MS.gene07440:CDS 20.0%
! AAAGGTAATCTCTTTAGATT+AGG + chr1.2:63545685-63545704 MS.gene07440:intron 25.0%
! AATTGATACAAATGCATCTA+AGG - chr1.2:63546051-63546070 None:intergenic 25.0%
! CATTTGTATCAATTGTAACA+GGG + chr1.2:63546057-63546076 MS.gene07440:CDS 25.0%
!! ATTTTTGTCTACAAAACCAA+CGG - chr1.2:63545888-63545907 None:intergenic 25.0%
!!! AATTTTTCATGGACTAATTC+AGG - chr1.2:63546275-63546294 None:intergenic 25.0%
!!! CATTGCCTATTTTACTTTTA+TGG + chr1.2:63546120-63546139 MS.gene07440:CDS 25.0%
!!! TTGCCTATTTTACTTTTATG+GGG + chr1.2:63546122-63546141 MS.gene07440:CDS 25.0%
GCATTTGTATCAATTGTAAC+AGG + chr1.2:63546056-63546075 MS.gene07440:CDS 30.0%
TACAAAACCAACGGTTAAAA+AGG - chr1.2:63545879-63545898 None:intergenic 30.0%
TCTCCCCATAAAAGTAAAAT+AGG - chr1.2:63546128-63546147 None:intergenic 30.0%
TTAAATGCAGAGAGAATTTG+AGG + chr1.2:63545755-63545774 MS.gene07440:intron 30.0%
! TGAACATTTTCTTGTTGAGA+AGG - chr1.2:63546206-63546225 None:intergenic 30.0%
!! ACAAAGCAATTGTTGATGAT+TGG + chr1.2:63545571-63545590 MS.gene07440:CDS 30.0%
!! TTTTTGAGCGTGTTAAACAA+AGG + chr1.2:63545667-63545686 MS.gene07440:CDS 30.0%
!!! TTGTCGTTTTCAAAGTTCAA+TGG + chr1.2:63545918-63545937 MS.gene07440:intron 30.0%
AAAATCAATCAGCCAAAGCA+AGG - chr1.2:63545616-63545635 None:intergenic 35.0%
AAATCAATCAGCCAAAGCAA+GGG - chr1.2:63545615-63545634 None:intergenic 35.0%
AACATCTTGAGTTCAGTGTA+TGG + chr1.2:63545778-63545797 MS.gene07440:CDS 35.0%
GAGTTCAGTGTATGGAAATA+AGG + chr1.2:63545786-63545805 MS.gene07440:CDS 35.0%
TCAAAGTTCAATGGAATCAC+CGG + chr1.2:63545927-63545946 MS.gene07440:intron 35.0%
TCCATGAAAAATTGCTTCCA+TGG + chr1.2:63546282-63546301 MS.gene07440:CDS 35.0%
TGAAAAATTGCTTCCATGGT+TGG + chr1.2:63546286-63546305 MS.gene07440:CDS 35.0%
TTCAACTGTTCATGCTTGAA+GGG + chr1.2:63546222-63546241 MS.gene07440:CDS 35.0%
TTCAGTGTATGGAAATAAGG+AGG + chr1.2:63545789-63545808 MS.gene07440:CDS 35.0%
! ACCATGGAAGCAATTTTTCA+TGG - chr1.2:63546286-63546305 None:intergenic 35.0%
! TTTGGTTCCTCAGATCATTA+GGG + chr1.2:63546004-63546023 MS.gene07440:intron 35.0%
!! GAAGAAAAGTACTTTCCAAC+AGG - chr1.2:63546179-63546198 None:intergenic 35.0%
!!! TTTTGGTTCCTCAGATCATT+AGG + chr1.2:63546003-63546022 MS.gene07440:intron 35.0%
AGGTCCATCAATTGGAGTAA+AGG - chr1.2:63546159-63546178 None:intergenic 40.0%
GAACTCGTGGAGGTAAATTA+AGG + chr1.2:63545823-63545842 MS.gene07440:intron 40.0%
GATCCCTTTACTCCAATTGA+TGG + chr1.2:63546152-63546171 MS.gene07440:CDS 40.0%
GGTCCATCAATTGGAGTAAA+GGG - chr1.2:63546158-63546177 None:intergenic 40.0%
GTTCAACTGTTCATGCTTGA+AGG + chr1.2:63546221-63546240 MS.gene07440:CDS 40.0%
TAATTCAGGCCTGTCATCAT+GGG - chr1.2:63546261-63546280 None:intergenic 40.0%
TCAACTGTTCATGCTTGAAG+GGG + chr1.2:63546223-63546242 MS.gene07440:CDS 40.0%
TTTCCAACAGGTCCATCAAT+TGG - chr1.2:63546167-63546186 None:intergenic 40.0%
! CTTCGTTCCTTTTTAACCGT+TGG + chr1.2:63545869-63545888 MS.gene07440:intron 40.0%
! TTGCAGAAGCAATTCCCTTT+TGG - chr1.2:63545647-63545666 None:intergenic 40.0%
AATTCAGGCCTGTCATCATG+GGG - chr1.2:63546260-63546279 None:intergenic 45.0%
AGTTACTCCTTCCCTTGCTT+TGG + chr1.2:63545601-63545620 MS.gene07440:CDS 45.0%
AGTTCAATGGAATCACCGGA+AGG + chr1.2:63545931-63545950 MS.gene07440:intron 45.0%
CTAATTCAGGCCTGTCATCA+TGG - chr1.2:63546262-63546281 None:intergenic 45.0%
GTATGGAAATAAGGAGGCTG+TGG + chr1.2:63545795-63545814 MS.gene07440:CDS 45.0%
TCCTCAGATCATTAGGGAAC+CGG + chr1.2:63546010-63546029 MS.gene07440:intron 45.0%
! ACTCCAATTGATGGACCTGT+TGG + chr1.2:63546161-63546180 MS.gene07440:CDS 45.0%
!!! ATTTTTGGCACCAGTTGCAC+CGG - chr1.2:63546098-63546117 None:intergenic 45.0%
ATCAATTGTAACAGGGCCGC+CGG + chr1.2:63546064-63546083 MS.gene07440:CDS 50.0%
CAATCAGCCAAAGCAAGGGA+AGG - chr1.2:63545611-63545630 None:intergenic 50.0%
CTGTTCATGCTTGAAGGGGT+TGG + chr1.2:63546227-63546246 MS.gene07440:CDS 50.0%
GACATTGTCCCCATGATGAC+AGG + chr1.2:63546249-63546268 MS.gene07440:CDS 50.0%
TGTGGATGATGAACTCGTGG+AGG + chr1.2:63545813-63545832 MS.gene07440:CDS 50.0%
AAGGCTCCTTCAGCATTCGC+CGG - chr1.2:63546032-63546051 None:intergenic 55.0%
GGCTGTGGATGATGAACTCG+TGG + chr1.2:63545810-63545829 MS.gene07440:CDS 55.0%
TCAATTGTAACAGGGCCGCC+GGG + chr1.2:63546065-63546084 MS.gene07440:CDS 55.0%
! GCCGGTTCCCTAATGATCTG+AGG - chr1.2:63546014-63546033 None:intergenic 55.0%
AGGGAACCGGCGAATGCTGA+AGG + chr1.2:63546023-63546042 MS.gene07440:CDS 60.0%
CAATTGTAACAGGGCCGCCG+GGG + chr1.2:63546066-63546085 MS.gene07440:CDS 60.0%
GGCACCAGTTGCACCGGATT+AGG - chr1.2:63546092-63546111 None:intergenic 60.0%
! AGTTGCACCGGATTAGGCCC+CGG - chr1.2:63546086-63546105 None:intergenic 60.0%
GGGGCCTAATCCGGTGCAAC+TGG + chr1.2:63546085-63546104 MS.gene07440:CDS 65.0%
! TGCACCGGATTAGGCCCCGG+CGG - chr1.2:63546083-63546102 None:intergenic 70.0%
AGGGCCGCCGGGGCCTAATC+CGG + chr1.2:63546076-63546095 MS.gene07440:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 63545564 63546319 63545564 ID=MS.gene07440
chr1.2 mRNA 63545564 63546319 63545564 ID=MS.gene07440.t1;Parent=MS.gene07440
chr1.2 exon 63545564 63545688 63545564 ID=MS.gene07440.t1.exon1;Parent=MS.gene07440.t1
chr1.2 CDS 63545564 63545688 63545564 ID=cds.MS.gene07440.t1;Parent=MS.gene07440.t1
chr1.2 exon 63545765 63545834 63545765 ID=MS.gene07440.t1.exon2;Parent=MS.gene07440.t1
chr1.2 CDS 63545765 63545834 63545765 ID=cds.MS.gene07440.t1;Parent=MS.gene07440.t1
chr1.2 exon 63546017 63546319 63546017 ID=MS.gene07440.t1.exon3;Parent=MS.gene07440.t1
chr1.2 CDS 63546017 63546319 63546017 ID=cds.MS.gene07440.t1;Parent=MS.gene07440.t1
Gene Sequence

>MS.gene07440

ATGAACAACAAAGCAATTGTTGATGATTGGAGAATCAAGTTACTCCTTCCCTTGCTTTGGCTGATTGATTTTTTATTGAACCAAAAGGGAATTGCTTCTGCAATTTTTGAGCGTGTTAAACAAAGGTAATCTCTTTAGATTAGGCCATAATTTCCCTAAATTTTTTGATAGGAAACATAAAACTGTATCGATTAAATGCAGAGAGAATTTGAGGAACATCTTGAGTTCAGTGTATGGAAATAAGGAGGCTGTGGATGATGAACTCGTGGAGGTAAATTAAGGCTATGATTGCTGTTTACTACTCTCTTCGTTCCTTTTTAACCGTTGGTTTTGTAGACAAAAATTGTTATTTATTTGTCGTTTTCAAAGTTCAATGGAATCACCGGAAGGAGTATTAATTTTTTTCTTCTTAAACAACATTTACTTATAAGAATTCATATTTTGGTTCCTCAGATCATTAGGGAACCGGCGAATGCTGAAGGAGCCTTAGATGCATTTGTATCAATTGTAACAGGGCCGCCGGGGCCTAATCCGGTGCAACTGGTGCCAAAAATATCATTGCCTATTTTACTTTTATGGGGAGATGAAGATCCCTTTACTCCAATTGATGGACCTGTTGGAAAGTACTTTTCTTCATTGCCTTCTCAACAAGAAAATGTTCAACTGTTCATGCTTGAAGGGGTTGGACATTGTCCCCATGATGACAGGCCTGAATTAGTCCATGAAAAATTGCTTCCATGGTTGGCAACTCTTTAA

Protein sequence

>MS.gene07440.t1

MNNKAIVDDWRIKLLLPLLWLIDFLLNQKGIASAIFERVKQRENLRNILSSVYGNKEAVDDELVEIIREPANAEGALDAFVSIVTGPPGPNPVQLVPKISLPILLLWGDEDPFTPIDGPVGKYFSSLPSQQENVQLFMLEGVGHCPHDDRPELVHEKLLPWLATL