AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46129


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46129.t1 MTR_7g062970 90.526 95 8 1 4 98 5 98 1.06e-58 175
MS.gene46129.t1 MTR_7g062970 90.526 95 8 1 4 98 5 98 1.07e-58 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46129.t1 AT5G02120 68.966 58 18 0 41 98 31 88 2.44e-22 84.0

Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAATTGAGGCTGTACTTT+TGG 0.250391 7.2:+40826310 None:intergenic
GATGAATGATGATGGTAATT+TGG 0.273708 7.2:+40826598 None:intergenic
CAATTTAAACCACCATTTCT+TGG 0.280965 7.2:-40826295 MS.gene46129:CDS
TTACCATCATCATTCATCTT+AGG 0.293810 7.2:-40826593 MS.gene46129:CDS
CTGCTGGTCTGTTCAAGTTA+TGG 0.307312 7.2:+40826522 None:intergenic
TTACAAAGACAGCTGAAATA+TGG 0.347950 7.2:-40826270 MS.gene46129:CDS
CTCTTCCTGTGAGGTGCTGC+TGG 0.363766 7.2:+40826506 None:intergenic
TTTGAAGGAACAAAAGTTGA+TGG 0.364124 7.2:+40826546 None:intergenic
AATTTAAACCACCATTTCTT+GGG 0.388545 7.2:-40826294 MS.gene46129:CDS
AACTCTAGAGCTTGCATGAT+AGG 0.431455 7.2:-40826247 MS.gene46129:CDS
GTCTTTGTAAACCCAAGAAA+TGG 0.476667 7.2:+40826283 None:intergenic
AGAAATGGTGGTTTAAATTG+AGG 0.476737 7.2:+40826298 None:intergenic
CAAGCAGTAAAACCTCCTTC+TGG 0.490122 7.2:-40826467 MS.gene46129:CDS
GCTTGCATGATAGGACTCAT+TGG 0.494425 7.2:-40826238 MS.gene46129:CDS
ACTCACAAGCTCTACAATGA+AGG 0.558455 7.2:+40826213 None:intergenic
TATAAAGTGCTTACACCAGA+AGG 0.567321 7.2:+40826452 None:intergenic
GTTCCTAAGATGAATGATGA+TGG 0.568966 7.2:+40826590 None:intergenic
TTTGTAAACCCAAGAAATGG+TGG 0.572116 7.2:+40826286 None:intergenic
TTTGGTTGATGAGGTAGCCA+TGG 0.573745 7.2:+40826616 None:intergenic
ACAGACCAGCAGCACCTCAC+AGG 0.606120 7.2:-40826511 MS.gene46129:CDS
GTTTGTAGAGTGAAGTGAAG+GGG 0.621170 7.2:+40826568 None:intergenic
TGATGGTAATTTGGTTGATG+AGG 0.628797 7.2:+40826607 None:intergenic
TGGTTTGTAGAGTGAAGTGA+AGG 0.633356 7.2:+40826566 None:intergenic
AAAGTGCTTACACCAGAAGG+AGG 0.671074 7.2:+40826455 None:intergenic
AAGGAAACTCTCTTCCTGTG+AGG 0.718755 7.2:+40826497 None:intergenic
GGTTTGTAGAGTGAAGTGAA+GGG 0.731952 7.2:+40826567 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTGCATTTTATATGTAAT+AGG - chr7.2:40826589-40826608 MS.gene46129:CDS 15.0%
!!! TAAGTTATTTATGAAGTTTT+TGG - chr7.2:40826387-40826406 MS.gene46129:intron 15.0%
!!! TTTGCATTTTATATGTAATA+GGG - chr7.2:40826590-40826609 MS.gene46129:CDS 15.0%
!!! ATGAAGTTTTTGGATTATTT+GGG - chr7.2:40826397-40826416 MS.gene46129:intron 20.0%
!!! TATGAAGTTTTTGGATTATT+TGG - chr7.2:40826396-40826415 MS.gene46129:intron 20.0%
!!! TTGCATTTTATATGTAATAG+GGG - chr7.2:40826591-40826610 MS.gene46129:CDS 20.0%
! AATTTAAACCACCATTTCTT+GGG - chr7.2:40826461-40826480 MS.gene46129:intron 25.0%
AGAAATGGTGGTTTAAATTG+AGG + chr7.2:40826460-40826479 None:intergenic 30.0%
CAATTTAAACCACCATTTCT+TGG - chr7.2:40826460-40826479 MS.gene46129:intron 30.0%
TTACAAAGACAGCTGAAATA+TGG - chr7.2:40826485-40826504 MS.gene46129:CDS 30.0%
TTACCATCATCATTCATCTT+AGG - chr7.2:40826162-40826181 MS.gene46129:intron 30.0%
TTTGAAGGAACAAAAGTTGA+TGG + chr7.2:40826212-40826231 None:intergenic 30.0%
! TAAATTGAGGCTGTACTTTT+GGG + chr7.2:40826447-40826466 None:intergenic 30.0%
! TTAAATTGAGGCTGTACTTT+TGG + chr7.2:40826448-40826467 None:intergenic 30.0%
! TTTTACTGCTTGAACAGTTA+AGG + chr7.2:40826280-40826299 None:intergenic 30.0%
!! GATGAATGATGATGGTAATT+TGG + chr7.2:40826160-40826179 None:intergenic 30.0%
!!! TGTTCAAGTTATGGTTTTGA+AGG + chr7.2:40826227-40826246 None:intergenic 30.0%
GTCTTTGTAAACCCAAGAAA+TGG + chr7.2:40826475-40826494 None:intergenic 35.0%
GTTCCTAAGATGAATGATGA+TGG + chr7.2:40826168-40826187 None:intergenic 35.0%
TTTGTAAACCCAAGAAATGG+TGG + chr7.2:40826472-40826491 None:intergenic 35.0%
! TATAAAGTGCTTACACCAGA+AGG + chr7.2:40826306-40826325 None:intergenic 35.0%
! TGATGGTAATTTGGTTGATG+AGG + chr7.2:40826151-40826170 None:intergenic 35.0%
!!! TTTGGGTTTGTGCTATTTTC+AGG - chr7.2:40826414-40826433 MS.gene46129:intron 35.0%
ACTCACAAGCTCTACAATGA+AGG + chr7.2:40826545-40826564 None:intergenic 40.0%
GGTTTGTAGAGTGAAGTGAA+GGG + chr7.2:40826191-40826210 None:intergenic 40.0%
GTTTGTAGAGTGAAGTGAAG+GGG + chr7.2:40826190-40826209 None:intergenic 40.0%
! TGGTTTGTAGAGTGAAGTGA+AGG + chr7.2:40826192-40826211 None:intergenic 40.0%
!! AACTCTAGAGCTTGCATGAT+AGG - chr7.2:40826508-40826527 MS.gene46129:CDS 40.0%
AAGGAAACTCTCTTCCTGTG+AGG + chr7.2:40826261-40826280 None:intergenic 45.0%
CAAGCAGTAAAACCTCCTTC+TGG - chr7.2:40826288-40826307 MS.gene46129:CDS 45.0%
GCTTGCATGATAGGACTCAT+TGG - chr7.2:40826517-40826536 MS.gene46129:CDS 45.0%
! AAAGTGCTTACACCAGAAGG+AGG + chr7.2:40826303-40826322 None:intergenic 45.0%
! CTGCTGGTCTGTTCAAGTTA+TGG + chr7.2:40826236-40826255 None:intergenic 45.0%
ACAGACCAGCAGCACCTCAC+AGG - chr7.2:40826244-40826263 MS.gene46129:CDS 60.0%
!! CTCTTCCTGTGAGGTGCTGC+TGG + chr7.2:40826252-40826271 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 40826141 40826636 40826141 ID=MS.gene46129
chr7.2 mRNA 40826141 40826636 40826141 ID=MS.gene46129.t1;Parent=MS.gene46129
chr7.2 exon 40826466 40826636 40826466 ID=MS.gene46129.t1.exon1;Parent=MS.gene46129.t1
chr7.2 CDS 40826466 40826636 40826466 ID=cds.MS.gene46129.t1;Parent=MS.gene46129.t1
chr7.2 exon 40826219 40826341 40826219 ID=MS.gene46129.t1.exon2;Parent=MS.gene46129.t1
chr7.2 CDS 40826219 40826341 40826219 ID=cds.MS.gene46129.t1;Parent=MS.gene46129.t1
chr7.2 exon 40826141 40826143 40826141 ID=MS.gene46129.t1.exon3;Parent=MS.gene46129.t1
chr7.2 CDS 40826141 40826143 40826141 ID=cds.MS.gene46129.t1;Parent=MS.gene46129.t1
Gene Sequence

>MS.gene46129

ATGGCTACCTCATCAACCAAATTACCATCATCATTCATCTTAGGAACCCCTTCACTTCACTCTACAAACCATCAACTTTTGTTCCTTCAAAACCATAACTTGAACAGACCAGCAGCACCTCACAGGAAGAGAGTTTCCTTAACTGTTCAAGCAGTAAAACCTCCTTCTGGTGTAAGCACTTTATATTTTTTAGTTTTTATGATATAGCATAGATATTAATATTTTCATTTTTGTTAATTTTTTTAATAAGTTATTTATGAAGTTTTTGGATTATTTGGGTTTGTGCTATTTTCAGGTTGAATTCCCAAAAGTACAGCCTCAATTTAAACCACCATTTCTTGGGTTTACAAAGACAGCTGAAATATGGAACTCTAGAGCTTGCATGATAGGACTCATTGGAACCTTCATTGTAGAGCTTGTGAGTATCTTCTATCTTCATTTTTAATCAATTTGCATTTTATATGTAATAGGGGTTTTTATGTTATTGAATTAGTGA

Protein sequence

>MS.gene46129.t1

MATSSTKLPSSFILGTPSLHSTNHQLLFLQNHNLNRPAAPHRKRVSLTVQAVKPPSGVEFPKVQPQFKPPFLGFTKTAEIWNSRACMIGLIGTFIVEL