Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0237260 | AES64280.1 | 92.248 | 129 | 9 | 1 | 1 | 128 | 1 | 129 | 3.68e-77 | 234 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0237260 | G7IFK5 | 92.248 | 129 | 9 | 1 | 1 | 128 | 1 | 129 | 1.76e-77 | 234 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003890 | Msa0237260 | 0.803900 | 2.795664e-49 | -8.615850e-47 |
| Msa0011960 | Msa0237260 | 0.804223 | 2.394261e-49 | -8.615850e-47 |
| Msa0012080 | Msa0237260 | 0.801204 | 1.005456e-48 | -8.615850e-47 |
| Msa0017510 | Msa0237260 | 0.826789 | 2.237006e-54 | -8.615850e-47 |
| Msa0025730 | Msa0237260 | 0.814158 | 1.776774e-51 | -8.615850e-47 |
| Msa0032580 | Msa0237260 | 0.817192 | 3.745740e-52 | -8.615850e-47 |
| Msa0037700 | Msa0237260 | 0.827818 | 1.267832e-54 | -8.615850e-47 |
| Msa0040690 | Msa0237260 | 0.817535 | 3.136040e-52 | -8.615850e-47 |
| Msa0044540 | Msa0237260 | -0.816008 | 6.900420e-52 | -8.615850e-47 |
| Msa0052800 | Msa0237260 | 0.816572 | 5.161368e-52 | -8.615850e-47 |
| Msa0054440 | Msa0237260 | 0.801120 | 1.046061e-48 | -8.615850e-47 |
| Msa0054480 | Msa0237260 | 0.827261 | 1.724698e-54 | -8.615850e-47 |
| Msa0074240 | Msa0237260 | 0.810171 | 1.316449e-50 | -8.615850e-47 |
| Msa0080200 | Msa0237260 | 0.801575 | 8.438623e-49 | -8.615850e-47 |
| Msa0081360 | Msa0237260 | 0.825080 | 5.695387e-54 | -8.615850e-47 |
| Msa0081480 | Msa0237260 | 0.826067 | 3.323706e-54 | -8.615850e-47 |
| Msa0081930 | Msa0237260 | 0.801037 | 1.087805e-48 | -8.615850e-47 |
| Msa0086650 | Msa0237260 | 0.804815 | 1.802446e-49 | -8.615850e-47 |
| Msa0091210 | Msa0237260 | -0.813490 | 2.493635e-51 | -8.615850e-47 |
| Msa0094470 | Msa0237260 | 0.811438 | 7.002099e-51 | -8.615850e-47 |
| Msa0101710 | Msa0237260 | 0.800569 | 1.355487e-48 | -8.615850e-47 |
| Msa0112750 | Msa0237260 | 0.805378 | 1.374034e-49 | -8.615850e-47 |
| Msa0123100 | Msa0237260 | 0.809392 | 1.935232e-50 | -8.615850e-47 |
| Msa0127050 | Msa0237260 | 0.806696 | 7.257819e-50 | -8.615850e-47 |
| Msa0133130 | Msa0237260 | 0.826213 | 3.068584e-54 | -8.615850e-47 |
| Msa0136070 | Msa0237260 | 0.823406 | 1.409165e-53 | -8.615850e-47 |
| Msa0136100 | Msa0237260 | 0.805812 | 1.114059e-49 | -8.615850e-47 |
| Msa0142660 | Msa0237260 | 0.971249 | 7.243528e-133 | -8.615850e-47 |
| Msa0150820 | Msa0237260 | 0.828043 | 1.119484e-54 | -8.615850e-47 |
| Msa0157290 | Msa0237260 | 0.827321 | 1.669131e-54 | -8.615850e-47 |
| Msa0160550 | Msa0237260 | 0.809587 | 1.758111e-50 | -8.615850e-47 |
| Msa0165660 | Msa0237260 | 0.800500 | 1.399882e-48 | -8.615850e-47 |
| Msa0171410 | Msa0237260 | 0.806856 | 6.711654e-50 | -8.615850e-47 |
| Msa0172970 | Msa0237260 | 0.810106 | 1.359464e-50 | -8.615850e-47 |
| Msa0173660 | Msa0237260 | 0.801987 | 6.946229e-49 | -8.615850e-47 |
| Msa0173990 | Msa0237260 | 0.840729 | 7.296654e-58 | -8.615850e-47 |
| Msa0178590 | Msa0237260 | 0.800721 | 1.261814e-48 | -8.615850e-47 |
| Msa0187120 | Msa0237260 | 0.821319 | 4.301283e-53 | -8.615850e-47 |
| Msa0195460 | Msa0237260 | 0.963460 | 4.114316e-122 | -8.615850e-47 |
| Msa0197660 | Msa0237260 | 0.822606 | 2.164947e-53 | -8.615850e-47 |
| Msa0212310 | Msa0237260 | 0.801027 | 1.092622e-48 | -8.615850e-47 |
| Msa0220720 | Msa0237260 | 0.831393 | 1.711941e-55 | -8.615850e-47 |
| Msa0222620 | Msa0237260 | 0.818990 | 1.468872e-52 | -8.615850e-47 |
| Msa0222890 | Msa0237260 | 0.811867 | 5.648632e-51 | -8.615850e-47 |
| Msa0228070 | Msa0237260 | 0.811844 | 5.713688e-51 | -8.615850e-47 |
| Msa0229380 | Msa0237260 | 0.813763 | 2.170582e-51 | -8.615850e-47 |
| Msa0237260 | Msa0239800 | 0.821413 | 4.091301e-53 | -8.615850e-47 |
| Msa0237260 | Msa0243100 | 0.807258 | 5.517500e-50 | -8.615850e-47 |
| Msa0237260 | Msa0255080 | 0.810271 | 1.252459e-50 | -8.615850e-47 |
| Msa0237260 | Msa0259370 | 0.805764 | 1.140403e-49 | -8.615850e-47 |
| Msa0237260 | Msa0260680 | 0.810636 | 1.044474e-50 | -8.615850e-47 |
| Msa0237260 | Msa0262310 | 0.818425 | 1.973820e-52 | -8.615850e-47 |
| Msa0237260 | Msa0264150 | 0.823022 | 1.731978e-53 | -8.615850e-47 |
| Msa0237260 | Msa0264930 | 0.802922 | 4.457874e-49 | -8.615850e-47 |
| Msa0237260 | Msa0271530 | 0.818727 | 1.685231e-52 | -8.615850e-47 |
| Msa0237260 | Msa0279790 | 0.808634 | 2.812692e-50 | -8.615850e-47 |
| Msa0237260 | Msa0280090 | 0.828057 | 1.110448e-54 | -8.615850e-47 |
| Msa0237260 | Msa0299870 | 0.823039 | 1.716663e-53 | -8.615850e-47 |
| Msa0237260 | Msa0309750 | 0.801034 | 1.089334e-48 | -8.615850e-47 |
| Msa0237260 | Msa0309770 | 0.804336 | 2.268663e-49 | -8.615850e-47 |
| Msa0237260 | Msa0317510 | 0.826112 | 3.244253e-54 | -8.615850e-47 |
| Msa0237260 | Msa0319170 | 0.807149 | 5.819872e-50 | -8.615850e-47 |
| Msa0237260 | Msa0336420 | 0.800373 | 1.485654e-48 | -8.615850e-47 |
| Msa0237260 | Msa0337240 | 0.802111 | 6.550081e-49 | -8.615850e-47 |
| Msa0237260 | Msa0337620 | 0.816162 | 6.374455e-52 | -8.615850e-47 |
| Msa0237260 | Msa0339250 | 0.801453 | 8.938757e-49 | -8.615850e-47 |
| Msa0237260 | Msa0340450 | 0.809151 | 2.180382e-50 | -8.615850e-47 |
| Msa0237260 | Msa0385580 | 0.827693 | 1.358686e-54 | -8.615850e-47 |
| Msa0237260 | Msa0398950 | 0.803107 | 4.082761e-49 | -8.615850e-47 |
| Msa0237260 | Msa0419820 | 0.804962 | 1.679123e-49 | -8.615850e-47 |
| Msa0237260 | Msa0428080 | 0.820722 | 5.903856e-53 | -8.615850e-47 |
| Msa0237260 | Msa0437560 | 0.811658 | 6.269995e-51 | -8.615850e-47 |
| Msa0237260 | Msa0479660 | 0.817345 | 3.460204e-52 | -8.615850e-47 |
| Msa0237260 | Msa0481530 | 0.818415 | 1.984230e-52 | -8.615850e-47 |
| Msa0237260 | Msa0483860 | 0.804827 | 1.791776e-49 | -8.615850e-47 |
| Msa0237260 | Msa0503580 | 0.813044 | 3.123966e-51 | -8.615850e-47 |
| Msa0237260 | Msa0512750 | 0.802104 | 6.571401e-49 | -8.615850e-47 |
| Msa0237260 | Msa0512900 | 0.801237 | 9.899491e-49 | -8.615850e-47 |
| Msa0237260 | Msa0529890 | 0.820482 | 6.699973e-53 | -8.615850e-47 |
| Msa0237260 | Msa0547720 | 0.806468 | 8.107357e-50 | -8.615850e-47 |
| Msa0237260 | Msa0555450 | 0.801868 | 7.348888e-49 | -8.615850e-47 |
| Msa0237260 | Msa0577500 | 0.800926 | 1.145850e-48 | -8.615850e-47 |
| Msa0237260 | Msa0585560 | 0.818461 | 1.936654e-52 | -8.615850e-47 |
| Msa0237260 | Msa0607050 | 0.816019 | 6.860467e-52 | -8.615850e-47 |
| Msa0237260 | Msa0610430 | 0.831580 | 1.539700e-55 | -8.615850e-47 |
| Msa0237260 | Msa0615440 | 0.804325 | 2.280261e-49 | -8.615850e-47 |
| Msa0237260 | Msa0623080 | 0.814228 | 1.713984e-51 | -8.615850e-47 |
| Msa0237260 | Msa0625720 | 0.818304 | 2.101720e-52 | -8.615850e-47 |
| Msa0237260 | Msa0657970 | 0.811055 | 8.477628e-51 | -8.615850e-47 |
| Msa0237260 | Msa0663390 | 0.821955 | 3.066185e-53 | -8.615850e-47 |
| Msa0237260 | Msa0673460 | 0.803402 | 3.545684e-49 | -8.615850e-47 |
| Msa0237260 | Msa0682900 | 0.803926 | 2.760480e-49 | -8.615850e-47 |
| Msa0237260 | Msa0684500 | 0.821987 | 3.014647e-53 | -8.615850e-47 |
| Msa0237260 | Msa0689260 | 0.816090 | 6.614480e-52 | -8.615850e-47 |
| Msa0237260 | Msa0692830 | 0.804177 | 2.448003e-49 | -8.615850e-47 |
| Msa0237260 | Msa0698490 | 0.809694 | 1.667046e-50 | -8.615850e-47 |
| Msa0237260 | Msa0698660 | 0.808873 | 2.500655e-50 | -8.615850e-47 |
| Msa0237260 | Msa0709500 | 0.819608 | 1.062242e-52 | -8.615850e-47 |
| Msa0237260 | Msa0711780 | 0.805889 | 1.073209e-49 | -8.615850e-47 |
| Msa0237260 | Msa0715700 | 0.838985 | 2.076545e-57 | -8.615850e-47 |
| Msa0237260 | Msa0727710 | 0.816704 | 4.822164e-52 | -8.615850e-47 |
| Msa0237260 | Msa0743720 | 0.835079 | 2.067750e-56 | -8.615850e-47 |
| Msa0237260 | Msa0745790 | 0.809351 | 1.975050e-50 | -8.615850e-47 |
| Msa0237260 | Msa0750880 | 0.815262 | 1.011447e-51 | -8.615850e-47 |
| Msa0237260 | Msa0790660 | -0.843985 | 1.000587e-58 | -8.615850e-47 |
| Msa0237260 | Msa0790850 | 0.817353 | 3.446718e-52 | -8.615850e-47 |
| Msa0237260 | Msa0793800 | 0.835284 | 1.834797e-56 | -8.615850e-47 |
| Msa0237260 | Msa0794670 | 0.810500 | 1.117821e-50 | -8.615850e-47 |
| Msa0237260 | Msa0796530 | 0.819140 | 1.357705e-52 | -8.615850e-47 |
| Msa0237260 | Msa0808080 | 0.807686 | 4.476684e-50 | -8.615850e-47 |
| Msa0237260 | Msa0808270 | 0.809332 | 1.993723e-50 | -8.615850e-47 |
| Msa0237260 | Msa0813720 | 0.807692 | 4.464952e-50 | -8.615850e-47 |
| Msa0237260 | Msa0813740 | 0.840969 | 6.312584e-58 | -8.615850e-47 |
| Msa0237260 | Msa0824680 | 0.801224 | 9.961133e-49 | -8.615850e-47 |
| Msa0237260 | Msa0827040 | 0.803047 | 4.199970e-49 | -8.615850e-47 |
| Msa0237260 | Msa0871270 | 0.806626 | 7.508668e-50 | -8.615850e-47 |
| Msa0237260 | Msa0880140 | 0.825126 | 5.557064e-54 | -8.615850e-47 |
| Msa0237260 | Msa0902760 | 0.838681 | 2.489182e-57 | -8.615850e-47 |
| Msa0237260 | Msa0936340 | 0.811240 | 7.729861e-51 | -8.615850e-47 |
| Msa0237260 | Msa0938220 | 0.819579 | 1.078579e-52 | -8.615850e-47 |
| Msa0237260 | Msa0947660 | 0.811073 | 8.402005e-51 | -8.615850e-47 |
| Msa0237260 | Msa0959360 | 0.814413 | 1.560568e-51 | -8.615850e-47 |
| Msa0237260 | Msa0968590 | 0.801488 | 8.794210e-49 | -8.615850e-47 |
| Msa0237260 | Msa0970030 | 0.823223 | 1.555000e-53 | -8.615850e-47 |
| Msa0237260 | Msa0970420 | 0.828850 | 7.146485e-55 | -8.615850e-47 |
| Msa0237260 | Msa0974070 | 0.812704 | 3.708072e-51 | -8.615850e-47 |
| Msa0237260 | Msa0993290 | 0.807102 | 5.954755e-50 | -8.615850e-47 |
| Msa0237260 | Msa0993330 | 0.804048 | 2.604370e-49 | -8.615850e-47 |
| Msa0237260 | Msa0993600 | 0.805372 | 1.378255e-49 | -8.615850e-47 |
| Msa0237260 | Msa0999970 | 0.800303 | 1.535397e-48 | -8.615850e-47 |
| Msa0237260 | Msa1003030 | 0.827811 | 1.272920e-54 | -8.615850e-47 |
| Msa0237260 | Msa1003750 | 0.811316 | 7.440214e-51 | -8.615850e-47 |
| Msa0237260 | Msa1006840 | 0.829865 | 4.053199e-55 | -8.615850e-47 |
| Msa0237260 | Msa1013400 | 0.837721 | 4.397099e-57 | -8.615850e-47 |
| Msa0237260 | Msa1031930 | 0.813690 | 2.252872e-51 | -8.615850e-47 |
| Msa0237260 | Msa1033410 | -0.802975 | 4.346528e-49 | -8.615850e-47 |
| Msa0237260 | Msa1040400 | 0.803908 | 2.785441e-49 | -8.615850e-47 |
| Msa0237260 | Msa1051200 | 0.819586 | 1.074287e-52 | -8.615850e-47 |
| Msa0237260 | Msa1066490 | 0.808891 | 2.478454e-50 | -8.615850e-47 |
| Msa0237260 | Msa1073070 | -0.811374 | 7.228952e-51 | -8.615850e-47 |
| Msa0237260 | Msa1075540 | 0.808688 | 2.738322e-50 | -8.615850e-47 |
| Msa0237260 | Msa1081150 | 0.815815 | 7.619701e-52 | -8.615850e-47 |
| Msa0237260 | Msa1082500 | 0.801964 | 7.022965e-49 | -8.615850e-47 |
| Msa0237260 | Msa1093530 | 0.839404 | 1.617322e-57 | -8.615850e-47 |
| Msa0237260 | Msa1104140 | 0.815960 | 7.072206e-52 | -8.615850e-47 |
| Msa0237260 | Msa1108280 | 0.800031 | 1.744068e-48 | -8.615850e-47 |
| Msa0237260 | Msa1123380 | 0.805610 | 1.228366e-49 | -8.615850e-47 |
| Msa0237260 | Msa1125760 | 0.811285 | 7.559057e-51 | -8.615850e-47 |
| Msa0237260 | Msa1131220 | 0.800489 | 1.406950e-48 | -8.615850e-47 |
| Msa0237260 | Msa1137700 | 0.801565 | 8.481908e-49 | -8.615850e-47 |
| Msa0237260 | Msa1147900 | 0.824770 | 6.739929e-54 | -8.615850e-47 |
| Msa0237260 | Msa1171360 | 0.821425 | 4.066516e-53 | -8.615850e-47 |
| Msa0237260 | Msa1173170 | 0.816452 | 5.489778e-52 | -8.615850e-47 |
| Msa0237260 | Msa1179460 | 0.804694 | 1.910204e-49 | -8.615850e-47 |
| Msa0237260 | Msa1193290 | 0.821764 | 3.395001e-53 | -8.615850e-47 |
| Msa0237260 | Msa1199220 | 0.815936 | 7.161343e-52 | -8.615850e-47 |
| Msa0237260 | Msa1210020 | 0.816073 | 6.674017e-52 | -8.615850e-47 |
| Msa0237260 | Msa1214890 | 0.805570 | 1.252175e-49 | -8.615850e-47 |
| Msa0237260 | Msa1218600 | 0.832339 | 1.000111e-55 | -8.615850e-47 |
| Msa0237260 | Msa1237940 | 0.803166 | 3.969344e-49 | -8.615850e-47 |
| Msa0237260 | Msa1255040 | -0.804360 | 2.242779e-49 | -8.615850e-47 |
| Msa0237260 | Msa1259490 | -0.811002 | 8.705049e-51 | -8.615850e-47 |
| Msa0237260 | Msa1295490 | -0.800834 | 1.196577e-48 | -8.615850e-47 |
| Msa0237260 | Msa1298510 | 0.811878 | 5.617236e-51 | -8.615850e-47 |
| Msa0237260 | Msa1299180 | -0.815475 | 9.071026e-52 | -8.615850e-47 |
| Msa0237260 | Msa1303250 | 0.800329 | 1.516933e-48 | -8.615850e-47 |
| Msa0237260 | Msa1305340 | 0.802651 | 5.070866e-49 | -8.615850e-47 |
| Msa0237260 | Msa1331910 | 0.800426 | 1.449248e-48 | -8.615850e-47 |
| Msa0237260 | Msa1332040 | -0.811141 | 8.119536e-51 | -8.615850e-47 |
| Msa0237260 | Msa1344130 | 0.818118 | 2.315983e-52 | -8.615850e-47 |
| Msa0237260 | Msa1353060 | 0.814615 | 1.407656e-51 | -8.615850e-47 |
| Msa0237260 | Msa1356440 | 0.819886 | 9.174564e-53 | -8.615850e-47 |
| Msa0237260 | Msa1358250 | 0.817116 | 3.895959e-52 | -8.615850e-47 |
| Msa0237260 | Msa1374120 | 0.801634 | 8.207794e-49 | -8.615850e-47 |
| Msa0237260 | Msa1385460 | 0.831903 | 1.281813e-55 | -8.615850e-47 |
| Msa0237260 | Msa1385670 | 0.811080 | 8.371530e-51 | -8.615850e-47 |
| Msa0237260 | Msa1392780 | 0.808420 | 3.125108e-50 | -8.615850e-47 |
| Msa0237260 | Msa1394290 | 0.824660 | 7.154698e-54 | -8.615850e-47 |
| Msa0237260 | Msa1423350 | 0.800322 | 1.521567e-48 | -8.615850e-47 |
| Msa0237260 | Msa1424750 | 0.842614 | 2.321545e-58 | -8.615850e-47 |
| Msa0237260 | Msa1444310 | 0.814167 | 1.768400e-51 | -8.615850e-47 |
| Msa0237260 | Msa1451160 | 0.804830 | 1.789489e-49 | -8.615850e-47 |
| Msa0237260 | Msa1457920 | 0.821264 | 4.429492e-53 | -8.615850e-47 |
| Msa0237260 | Msa1466540 | 0.824387 | 8.295573e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0237260 | MtrunA17_Chr2g0286621 | 92.248 | 129 | 9 | 1 | 1 | 128 | 1 | 129 | 3.38e-81 | 234 |
| Msa0237260 | MtrunA17_Chr2g0286631 | 80.435 | 138 | 17 | 3 | 1 | 128 | 1 | 138 | 1.77e-57 | 174 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAATGGTTAATGTTAATT+TGG | 0.155770 | 2_2:-10336802 | Msa0237260:CDS |
| GATTCTGAAGGTGACCTTAT+TGG | 0.270756 | 2_2:-10337056 | Msa0237260:CDS |
| AAGGAAGATGAGAATGATAA+AGG | 0.271877 | 2_2:-10336930 | Msa0237260:CDS |
| TCATAAAGAAATGAATGATA+TGG | 0.276443 | 2_2:-10337120 | None:intergenic |
| ATGTTGATGATAAACTATTT+TGG | 0.278652 | 2_2:-10336776 | Msa0237260:CDS |
| TACCCTGCTGCTAATTACTT+TGG | 0.359178 | 2_2:-10337020 | Msa0237260:CDS |
| TTCATCTTTGTTGCTTATTG+AGG | 0.383420 | 2_2:-10337087 | Msa0237260:CDS |
| ATAGAGTAAAGAAGCCAATA+AGG | 0.396823 | 2_2:+10337042 | None:intergenic |
| ACCCTGCTGCTAATTACTTT+GGG | 0.397321 | 2_2:-10337019 | Msa0237260:CDS |
| TGGGAGATATGTATGGAAGT+AGG | 0.434821 | 2_2:-10336756 | Msa0237260:CDS |
| GGGAGATATGTATGGAAGTA+GGG | 0.453186 | 2_2:-10336755 | Msa0237260:CDS |
| TGGTATGTGTAGCTTAGTTA+AGG | 0.466313 | 2_2:-10336949 | Msa0237260:CDS |
| AGGAAGATGAGAATGATAAA+GGG | 0.474639 | 2_2:-10336929 | Msa0237260:CDS |
| GATGAGAATGATAAAGGGTC+AGG | 0.478591 | 2_2:-10336924 | Msa0237260:CDS |
| TGCTAATTACTTTGGGCACA+AGG | 0.479009 | 2_2:-10337012 | Msa0237260:CDS |
| TGCCCAAAGTAATTAGCAGC+AGG | 0.488758 | 2_2:+10337017 | None:intergenic |
| TCCTCATCATCAACAAGTAA+AGG | 0.503410 | 2_2:+10336831 | None:intergenic |
| ACCTTTACTTGTTGATGATG+AGG | 0.506285 | 2_2:-10336832 | Msa0237260:CDS |
| ACCATCATTGTCACCCTCAC+AGG | 0.527345 | 2_2:+10336968 | None:intergenic |
| AGAAGAGCATTCTATTTCCA+TGG | 0.535708 | 2_2:+10336857 | None:intergenic |
| TGATGAGGAGGAAATAAGAA+TGG | 0.564678 | 2_2:-10336817 | Msa0237260:CDS |
| TTTACTTGTTGATGATGAGG+AGG | 0.584354 | 2_2:-10336829 | Msa0237260:CDS |
| GTGGTTATCAGATGCAACCA+TGG | 0.627194 | 2_2:-10336874 | Msa0237260:CDS |
| ACCTGTGAGGGTGACAATGA+TGG | 0.639274 | 2_2:-10336969 | Msa0237260:CDS |
| GAGGACAGTGCAGATTCTGA+AGG | 0.642136 | 2_2:-10337068 | Msa0237260:CDS |
| GCCCAAAGTAATTAGCAGCA+GGG | 0.657577 | 2_2:+10337018 | None:intergenic |
| GCTGAGTCATGTACCTGTGA+GGG | 0.671916 | 2_2:-10336981 | Msa0237260:CDS |
| TGCTGAGTCATGTACCTGTG+AGG | 0.698564 | 2_2:-10336982 | Msa0237260:CDS |
| GGTTGCATCTGATAACCACA+TGG | 0.727761 | 2_2:+10336878 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAATGGTTAATGTTAATT+TGG | - | chr2_2:10337054-10337073 | Msa0237260:CDS | 20.0% |
| !!! | ATGTTGATGATAAACTATTT+TGG | - | chr2_2:10337080-10337099 | Msa0237260:CDS | 20.0% |
| !!! | TGTTGATGATAAACTATTTT+GGG | - | chr2_2:10337081-10337100 | Msa0237260:CDS | 20.0% |
| ! | TTCATCTTTGTTGCTTATTG+AGG | - | chr2_2:10336769-10336788 | Msa0237260:CDS | 30.0% |
| ATAGAGTAAAGAAGCCAATA+AGG | + | chr2_2:10336817-10336836 | None:intergenic | 30.0% | |
| AAGGAAGATGAGAATGATAA+AGG | - | chr2_2:10336926-10336945 | Msa0237260:CDS | 30.0% | |
| AGGAAGATGAGAATGATAAA+GGG | - | chr2_2:10336927-10336946 | Msa0237260:CDS | 30.0% | |
| !! | ACTATTTTGGGAGATATGTA+TGG | - | chr2_2:10337093-10337112 | Msa0237260:CDS | 30.0% |
| TGGTATGTGTAGCTTAGTTA+AGG | - | chr2_2:10336907-10336926 | Msa0237260:CDS | 35.0% | |
| !! | AGAAGAGCATTCTATTTCCA+TGG | + | chr2_2:10337002-10337021 | None:intergenic | 35.0% |
| !! | ACCTTTACTTGTTGATGATG+AGG | - | chr2_2:10337024-10337043 | Msa0237260:CDS | 35.0% |
| TCCTCATCATCAACAAGTAA+AGG | + | chr2_2:10337028-10337047 | None:intergenic | 35.0% | |
| !! | TTTACTTGTTGATGATGAGG+AGG | - | chr2_2:10337027-10337046 | Msa0237260:CDS | 35.0% |
| TGATGAGGAGGAAATAAGAA+TGG | - | chr2_2:10337039-10337058 | Msa0237260:CDS | 35.0% | |
| ! | GATTCTGAAGGTGACCTTAT+TGG | - | chr2_2:10336800-10336819 | Msa0237260:CDS | 40.0% |
| TACCCTGCTGCTAATTACTT+TGG | - | chr2_2:10336836-10336855 | Msa0237260:CDS | 40.0% | |
| ACCCTGCTGCTAATTACTTT+GGG | - | chr2_2:10336837-10336856 | Msa0237260:CDS | 40.0% | |
| TGCTAATTACTTTGGGCACA+AGG | - | chr2_2:10336844-10336863 | Msa0237260:CDS | 40.0% | |
| GATGAGAATGATAAAGGGTC+AGG | - | chr2_2:10336932-10336951 | Msa0237260:CDS | 40.0% | |
| TGGGAGATATGTATGGAAGT+AGG | - | chr2_2:10337100-10337119 | Msa0237260:CDS | 40.0% | |
| GGGAGATATGTATGGAAGTA+GGG | - | chr2_2:10337101-10337120 | Msa0237260:CDS | 40.0% | |
| GCCCAAAGTAATTAGCAGCA+GGG | + | chr2_2:10336841-10336860 | None:intergenic | 45.0% | |
| TGCCCAAAGTAATTAGCAGC+AGG | + | chr2_2:10336842-10336861 | None:intergenic | 45.0% | |
| !!! | GCTGTTTTAGCTCTACCATG+TGG | - | chr2_2:10336963-10336982 | Msa0237260:CDS | 45.0% |
| GGTTGCATCTGATAACCACA+TGG | + | chr2_2:10336981-10337000 | None:intergenic | 45.0% | |
| GTGGTTATCAGATGCAACCA+TGG | - | chr2_2:10336982-10337001 | Msa0237260:CDS | 45.0% | |
| GAGGACAGTGCAGATTCTGA+AGG | - | chr2_2:10336788-10336807 | Msa0237260:CDS | 50.0% | |
| TGCTGAGTCATGTACCTGTG+AGG | - | chr2_2:10336874-10336893 | Msa0237260:CDS | 50.0% | |
| GCTGAGTCATGTACCTGTGA+GGG | - | chr2_2:10336875-10336894 | Msa0237260:CDS | 50.0% | |
| ACCTGTGAGGGTGACAATGA+TGG | - | chr2_2:10336887-10336906 | Msa0237260:CDS | 50.0% | |
| ACCATCATTGTCACCCTCAC+AGG | + | chr2_2:10336891-10336910 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 10336746 | 10337132 | 10336746 | ID=Msa0237260;Name=Msa0237260 |
| chr2_2 | mRNA | 10336746 | 10337132 | 10336746 | ID=Msa0237260-mRNA-1;Parent=Msa0237260;Name=Msa0237260-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|128 |
| chr2_2 | exon | 10336746 | 10337132 | 10336746 | ID=Msa0237260-mRNA-1:exon:4477;Parent=Msa0237260-mRNA-1 |
| chr2_2 | CDS | 10336746 | 10337132 | 10336746 | ID=Msa0237260-mRNA-1:cds;Parent=Msa0237260-mRNA-1 |
| Gene Sequence |
| Protein sequence |
