AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0195460


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0195460 MtrunA17_Chr2g0286621 92.248 129 9 1 1 128 1 129 3.38e-81 234
Msa0195460 MtrunA17_Chr2g0286631 80.435 138 17 3 1 128 1 138 1.77e-57 174
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATGGTTAATGTTAATT+TGG 0.155770 2_1:-12300526 Msa0195460:CDS
GATTCTGAAGGTGACCTTAT+TGG 0.270756 2_1:-12300780 Msa0195460:CDS
AAGGAAGATGAGAATGATAA+AGG 0.271877 2_1:-12300654 Msa0195460:CDS
TCATAAAGAAATGAATGATA+TGG 0.276443 2_1:-12300844 None:intergenic
ATGTTGATGATAAACTATTT+TGG 0.278652 2_1:-12300500 Msa0195460:CDS
TACCCTGCTGCTAATTACTT+TGG 0.359178 2_1:-12300744 Msa0195460:CDS
TTCATCTTTGTTGCTTATTG+AGG 0.383420 2_1:-12300811 Msa0195460:CDS
ATAGAGTAAAGAAGCCAATA+AGG 0.396823 2_1:+12300766 None:intergenic
ACCCTGCTGCTAATTACTTT+GGG 0.397321 2_1:-12300743 Msa0195460:CDS
TGGGAGATATGTATGGAAGT+AGG 0.434821 2_1:-12300480 Msa0195460:CDS
GGGAGATATGTATGGAAGTA+GGG 0.453186 2_1:-12300479 Msa0195460:CDS
TGGTATGTGTAGCTTAGTTA+AGG 0.466313 2_1:-12300673 Msa0195460:CDS
AGGAAGATGAGAATGATAAA+GGG 0.474639 2_1:-12300653 Msa0195460:CDS
GATGAGAATGATAAAGGGTC+AGG 0.478591 2_1:-12300648 Msa0195460:CDS
TGCTAATTACTTTGGGCACA+AGG 0.479009 2_1:-12300736 Msa0195460:CDS
TGCCCAAAGTAATTAGCAGC+AGG 0.488758 2_1:+12300741 None:intergenic
TCCTCATCATCAACAAGTAA+AGG 0.503410 2_1:+12300555 None:intergenic
ACCTTTACTTGTTGATGATG+AGG 0.506285 2_1:-12300556 Msa0195460:CDS
ACCATCATTGTCACCCTCAC+AGG 0.527345 2_1:+12300692 None:intergenic
AGAAGAGCATTCTATTTCCA+TGG 0.535708 2_1:+12300581 None:intergenic
TGATGAGGAGGAAATAAGAA+TGG 0.564678 2_1:-12300541 Msa0195460:CDS
TTTACTTGTTGATGATGAGG+AGG 0.584354 2_1:-12300553 Msa0195460:CDS
GTGGTTATCAGATGCAACCA+TGG 0.627194 2_1:-12300598 Msa0195460:CDS
ACCTGTGAGGGTGACAATGA+TGG 0.639274 2_1:-12300693 Msa0195460:CDS
GAGGACAGTGCAGATTCTGA+AGG 0.642136 2_1:-12300792 Msa0195460:CDS
GCCCAAAGTAATTAGCAGCA+GGG 0.657577 2_1:+12300742 None:intergenic
GCTGAGTCATGTACCTGTGA+GGG 0.671916 2_1:-12300705 Msa0195460:CDS
TGCTGAGTCATGTACCTGTG+AGG 0.698564 2_1:-12300706 Msa0195460:CDS
GGTTGCATCTGATAACCACA+TGG 0.727761 2_1:+12300602 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAATGGTTAATGTTAATT+TGG - chr2_1:12300778-12300797 Msa0195460:CDS 20.0%
!!! ATGTTGATGATAAACTATTT+TGG - chr2_1:12300804-12300823 Msa0195460:CDS 20.0%
!!! TGTTGATGATAAACTATTTT+GGG - chr2_1:12300805-12300824 Msa0195460:CDS 20.0%
! TTCATCTTTGTTGCTTATTG+AGG - chr2_1:12300493-12300512 Msa0195460:CDS 30.0%
ATAGAGTAAAGAAGCCAATA+AGG + chr2_1:12300541-12300560 None:intergenic 30.0%
AAGGAAGATGAGAATGATAA+AGG - chr2_1:12300650-12300669 Msa0195460:CDS 30.0%
AGGAAGATGAGAATGATAAA+GGG - chr2_1:12300651-12300670 Msa0195460:CDS 30.0%
!! ACTATTTTGGGAGATATGTA+TGG - chr2_1:12300817-12300836 Msa0195460:CDS 30.0%
TGGTATGTGTAGCTTAGTTA+AGG - chr2_1:12300631-12300650 Msa0195460:CDS 35.0%
!! AGAAGAGCATTCTATTTCCA+TGG + chr2_1:12300726-12300745 None:intergenic 35.0%
!! ACCTTTACTTGTTGATGATG+AGG - chr2_1:12300748-12300767 Msa0195460:CDS 35.0%
TCCTCATCATCAACAAGTAA+AGG + chr2_1:12300752-12300771 None:intergenic 35.0%
!! TTTACTTGTTGATGATGAGG+AGG - chr2_1:12300751-12300770 Msa0195460:CDS 35.0%
TGATGAGGAGGAAATAAGAA+TGG - chr2_1:12300763-12300782 Msa0195460:CDS 35.0%
! GATTCTGAAGGTGACCTTAT+TGG - chr2_1:12300524-12300543 Msa0195460:CDS 40.0%
TACCCTGCTGCTAATTACTT+TGG - chr2_1:12300560-12300579 Msa0195460:CDS 40.0%
ACCCTGCTGCTAATTACTTT+GGG - chr2_1:12300561-12300580 Msa0195460:CDS 40.0%
TGCTAATTACTTTGGGCACA+AGG - chr2_1:12300568-12300587 Msa0195460:CDS 40.0%
GATGAGAATGATAAAGGGTC+AGG - chr2_1:12300656-12300675 Msa0195460:CDS 40.0%
TGGGAGATATGTATGGAAGT+AGG - chr2_1:12300824-12300843 Msa0195460:CDS 40.0%
GGGAGATATGTATGGAAGTA+GGG - chr2_1:12300825-12300844 Msa0195460:CDS 40.0%
GCCCAAAGTAATTAGCAGCA+GGG + chr2_1:12300565-12300584 None:intergenic 45.0%
TGCCCAAAGTAATTAGCAGC+AGG + chr2_1:12300566-12300585 None:intergenic 45.0%
!!! GCTGTTTTAGCTCTACCATG+TGG - chr2_1:12300687-12300706 Msa0195460:CDS 45.0%
GGTTGCATCTGATAACCACA+TGG + chr2_1:12300705-12300724 None:intergenic 45.0%
GTGGTTATCAGATGCAACCA+TGG - chr2_1:12300706-12300725 Msa0195460:CDS 45.0%
GAGGACAGTGCAGATTCTGA+AGG - chr2_1:12300512-12300531 Msa0195460:CDS 50.0%
TGCTGAGTCATGTACCTGTG+AGG - chr2_1:12300598-12300617 Msa0195460:CDS 50.0%
GCTGAGTCATGTACCTGTGA+GGG - chr2_1:12300599-12300618 Msa0195460:CDS 50.0%
ACCTGTGAGGGTGACAATGA+TGG - chr2_1:12300611-12300630 Msa0195460:CDS 50.0%
ACCATCATTGTCACCCTCAC+AGG + chr2_1:12300615-12300634 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 12300470 12300856 12300470 ID=Msa0195460;Name=Msa0195460
chr2_1 mRNA 12300470 12300856 12300470 ID=Msa0195460-mRNA-1;Parent=Msa0195460;Name=Msa0195460-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|128
chr2_1 exon 12300470 12300856 12300470 ID=Msa0195460-mRNA-1:exon:5393;Parent=Msa0195460-mRNA-1
chr2_1 CDS 12300470 12300856 12300470 ID=Msa0195460-mRNA-1:cds;Parent=Msa0195460-mRNA-1
Gene Sequence

>Msa0195460

ATGAATGATATGGATGTATCATGTTCATCTTTGTTGCTTATTGAGGACAGTGCAGATTCTGAAGGTGACCTTATTGGCTTCTTTACTCTATACCCTGCTGCTAATTACTTTGGGCACAAGGAAGATGATGCTGAGTCATGTACCTGTGAGGGTGACAATGATGGTATGTGTAGCTTAGTTAAGGAAGATGAGAATGATAAAGGGTCAGGTGAATTTTGCTGTTTTAGCTCTACCATGTGGTTATCAGATGCAACCATGGAAATAGAATGCTCTTCTTCACCTTTACTTGTTGATGATGAGGAGGAAATAAGAATGGTTAATGTTAATTTGGATGATGTTGATGATAAACTATTTTGGGAGATATGTATGGAAGTAGGGTACCCTTGA

Protein sequence

>Msa0195460

MNDMDVSCSSLLLIEDSADSEGDLIGFFTLYPAANYFGHKEDDAESCTCEGDNDGMCSLVKEDENDKGSGEFCCFSSTMWLSDATMEIECSSSPLLVDDEEEIRMVNVNLDDVDDKLFWEICMEVGYP*