AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene39421


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39421.t1 MTR_4g089080 96.891 193 6 0 1 193 1 193 4.05e-133 371
MS.gene39421.t1 MTR_3g026020 40.000 175 92 4 25 191 18 187 8.25e-21 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene39421.t1 AT1G74730 64.062 192 67 2 3 193 8 198 2.27e-65 199
MS.gene39421.t1 AT5G08050 40.000 125 67 2 72 193 39 158 6.15e-15 69.3

Find 47 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTGGTGTTGGGGCTGTT+GGG 0.261115 4.2:+21888548 MS.gene39421:CDS
TGCGGTCTCTCCTTCCGTTT+TGG 0.280512 4.2:+21888423 MS.gene39421:CDS
GCTCTTGGTGTTGGGGCTGT+TGG 0.285475 4.2:+21888547 MS.gene39421:CDS
TTGCTGCTGCTCTTGGTGTT+GGG 0.287275 4.2:+21888539 MS.gene39421:CDS
TCTGTCACTTCCGACGCTTT+TGG 0.320526 4.2:+21888450 MS.gene39421:CDS
AAAGACGGTGAAAGGTTTAT+TGG 0.327341 4.2:-21888046 None:intergenic
GTTGCTGCTGCTCTTGGTGT+TGG 0.359407 4.2:+21888538 MS.gene39421:CDS
TGAACAAGTACAACGATAAC+TGG 0.365581 4.2:+21888176 MS.gene39421:CDS
GCTGTTGTTGCTGCTGCTCT+TGG 0.375504 4.2:+21888532 MS.gene39421:CDS
GGGGCTGTTGGGTTGTTCGC+TGG 0.385149 4.2:+21888559 MS.gene39421:CDS
TTATCCAAAGCTGAAGAATT+CGG 0.394470 4.2:+21888316 MS.gene39421:CDS
GACGCCGAATTCTTCAGCTT+TGG 0.394886 4.2:-21888320 None:intergenic
TTCGCTGGATCTGTTGTTCT+CGG 0.420848 4.2:+21888574 MS.gene39421:CDS
CTGGATCTGTTGTTCTCGGC+GGG 0.425729 4.2:+21888578 MS.gene39421:CDS
TGCTGCTGCTCTTGGTGTTG+GGG 0.441410 4.2:+21888540 MS.gene39421:CDS
AAAGGTTTATTGGAGGAAGT+AGG 0.473704 4.2:-21888036 None:intergenic
AAGGTTTATTGGAGGAAGTA+GGG 0.490170 4.2:-21888035 None:intergenic
AACTGGAAGAAACAATGGTT+CGG 0.517807 4.2:+21888193 MS.gene39421:CDS
ACCTTTCACCGTCTTTGCCA+TGG 0.518610 4.2:+21888053 MS.gene39421:CDS
TACCGCTCTTGTTGTAGTAC+AGG 0.524783 4.2:+21888510 MS.gene39421:CDS
AAGAAACAATGGTTCGGAGC+AGG 0.525520 4.2:+21888199 MS.gene39421:CDS
TGGAGAGCTGAAGTTGGAGC+AGG 0.527987 4.2:-21887961 None:intergenic
ACAAGAGCGGTAGTATCATC+AGG 0.531633 4.2:-21888499 None:intergenic
AGCGATAGTGACGGAGGGTG+CGG 0.535126 4.2:-21888008 None:intergenic
TTTGGGAGCCATGGCAAAGA+CGG 0.549608 4.2:-21888061 None:intergenic
GACGGTGAAAGGTTTATTGG+AGG 0.550094 4.2:-21888043 None:intergenic
TGAAGATGGAGAGCTGAAGT+TGG 0.551350 4.2:-21887967 None:intergenic
AAGTGACAGAGAAGCCAAAA+CGG 0.551577 4.2:-21888437 None:intergenic
GCCATGGCAAAGACGGTGAA+AGG 0.551635 4.2:-21888054 None:intergenic
AGGAAGCGAAGAAGTAGAAG+TGG 0.553396 4.2:+21888234 MS.gene39421:CDS
CTCTCTTCAATCGAGAAACT+AGG 0.554038 4.2:+21888346 MS.gene39421:CDS
GACAGAGAAGCCAAAACGGA+AGG 0.555736 4.2:-21888433 None:intergenic
GGAGGGTGCGGTGGTTAGTG+TGG 0.562291 4.2:-21887996 None:intergenic
TTTGGCTGCTGTTGCGACGG+TGG 0.563007 4.2:+21888468 MS.gene39421:CDS
TAGTGTGGATGTGTTGAAGA+TGG 0.568146 4.2:-21887981 None:intergenic
TGTTCTCGGCGGGTTGCAAG+AGG 0.570576 4.2:+21888588 MS.gene39421:CDS
AGTTGGAGCAGGAACAGCCA+TGG 0.616976 4.2:-21887950 None:intergenic
ACGATAACTGGAAGAAACAA+TGG 0.622411 4.2:+21888188 MS.gene39421:CDS
GATAGTGACGGAGGGTGCGG+TGG 0.630396 4.2:-21888005 None:intergenic
TAGGGAATGAGCGATAGTGA+CGG 0.635156 4.2:-21888017 None:intergenic
GCAACAGCAGCCAAAAGCGT+CGG 0.640980 4.2:-21888460 None:intergenic
GGAATGAGCGATAGTGACGG+AGG 0.658654 4.2:-21888014 None:intergenic
GCTGGATCTGTTGTTCTCGG+CGG 0.659281 4.2:+21888577 MS.gene39421:CDS
GAATGAGCGATAGTGACGGA+GGG 0.663461 4.2:-21888013 None:intergenic
GGAGCAGGAATATTCTACGA+AGG 0.698664 4.2:+21888214 MS.gene39421:CDS
AGCCTGTACTACAACAAGAG+CGG 0.708420 4.2:-21888512 None:intergenic
CTTATTAGAGAAACTCGCTG+CGG 0.756406 4.2:+21888405 MS.gene39421:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTGAATTTATGTTAA+TGG + chr4.2:21888143-21888162 MS.gene39421:intron 10.0%
!! AAATAATTACGAAAATGATG+AGG - chr4.2:21888109-21888128 None:intergenic 20.0%
!!! TGAATTTATGTTAATGGATA+TGG + chr4.2:21888149-21888168 MS.gene39421:intron 20.0%
!! TTTATGTTAATGGATATGGA+AGG + chr4.2:21888153-21888172 MS.gene39421:intron 25.0%
TTATCCAAAGCTGAAGAATT+CGG + chr4.2:21888316-21888335 MS.gene39421:CDS 30.0%
! TGTGTATGTACCTTCTTTTT+GGG - chr4.2:21888081-21888100 None:intergenic 30.0%
! TTGTGTATGTACCTTCTTTT+TGG - chr4.2:21888082-21888101 None:intergenic 30.0%
!!! GAAACTAGGACTTTTTTCTA+AGG + chr4.2:21888360-21888379 MS.gene39421:CDS 30.0%
AAAGACGGTGAAAGGTTTAT+TGG - chr4.2:21888049-21888068 None:intergenic 35.0%
AAAGGTTTATTGGAGGAAGT+AGG - chr4.2:21888039-21888058 None:intergenic 35.0%
AACTGGAAGAAACAATGGTT+CGG + chr4.2:21888193-21888212 MS.gene39421:CDS 35.0%
AAGGTTTATTGGAGGAAGTA+GGG - chr4.2:21888038-21888057 None:intergenic 35.0%
ACGATAACTGGAAGAAACAA+TGG + chr4.2:21888188-21888207 MS.gene39421:CDS 35.0%
TGAACAAGTACAACGATAAC+TGG + chr4.2:21888176-21888195 MS.gene39421:CDS 35.0%
TTAAGCAACGTTGAAAAAGC+AGG + chr4.2:21888289-21888308 MS.gene39421:CDS 35.0%
!!! ACTAGGACTTTTTTCTAAGG+CGG + chr4.2:21888363-21888382 MS.gene39421:CDS 35.0%
AAGTGACAGAGAAGCCAAAA+CGG - chr4.2:21888440-21888459 None:intergenic 40.0%
CTCTCTTCAATCGAGAAACT+AGG + chr4.2:21888346-21888365 MS.gene39421:CDS 40.0%
CTTATTAGAGAAACTCGCTG+CGG + chr4.2:21888405-21888424 MS.gene39421:CDS 40.0%
TAGTGTGGATGTGTTGAAGA+TGG - chr4.2:21887984-21888003 None:intergenic 40.0%
!!! TTTTCTAAGGCGGAAGAACT+TGG + chr4.2:21888373-21888392 MS.gene39421:CDS 40.0%
ACAAGAGCGGTAGTATCATC+AGG - chr4.2:21888502-21888521 None:intergenic 45.0%
AGCCTGTACTACAACAAGAG+CGG - chr4.2:21888515-21888534 None:intergenic 45.0%
AGGAAGCGAAGAAGTAGAAG+TGG + chr4.2:21888234-21888253 MS.gene39421:CDS 45.0%
GACGGTGAAAGGTTTATTGG+AGG - chr4.2:21888046-21888065 None:intergenic 45.0%
GGAGCAGGAATATTCTACGA+AGG + chr4.2:21888214-21888233 MS.gene39421:CDS 45.0%
TAGGGAATGAGCGATAGTGA+CGG - chr4.2:21888020-21888039 None:intergenic 45.0%
TGAAGATGGAGAGCTGAAGT+TGG - chr4.2:21887970-21887989 None:intergenic 45.0%
TTCGCTGGATCTGTTGTTCT+CGG + chr4.2:21888574-21888593 MS.gene39421:CDS 45.0%
! AAGAAACAATGGTTCGGAGC+AGG + chr4.2:21888199-21888218 MS.gene39421:CDS 45.0%
! TACCGCTCTTGTTGTAGTAC+AGG + chr4.2:21888510-21888529 MS.gene39421:CDS 45.0%
!! TACCTTCTTTTTGGGAGCCA+TGG - chr4.2:21888073-21888092 None:intergenic 45.0%
ACCTTTCACCGTCTTTGCCA+TGG + chr4.2:21888053-21888072 MS.gene39421:CDS 50.0%
GACAGAGAAGCCAAAACGGA+AGG - chr4.2:21888436-21888455 None:intergenic 50.0%
GACGCCGAATTCTTCAGCTT+TGG - chr4.2:21888323-21888342 None:intergenic 50.0%
TGCCATGGCTCCCAAAAAGA+AGG + chr4.2:21888068-21888087 MS.gene39421:CDS 50.0%
TTTGGGAGCCATGGCAAAGA+CGG - chr4.2:21888064-21888083 None:intergenic 50.0%
! GAATGAGCGATAGTGACGGA+GGG - chr4.2:21888016-21888035 None:intergenic 50.0%
!! TCTGTCACTTCCGACGCTTT+TGG + chr4.2:21888450-21888469 MS.gene39421:CDS 50.0%
!! TTGCTGCTGCTCTTGGTGTT+GGG + chr4.2:21888539-21888558 MS.gene39421:CDS 50.0%
CTGGATCTGTTGTTCTCGGC+GGG + chr4.2:21888578-21888597 MS.gene39421:CDS 55.0%
GCCATGGCAAAGACGGTGAA+AGG - chr4.2:21888057-21888076 None:intergenic 55.0%
GCTGGATCTGTTGTTCTCGG+CGG + chr4.2:21888577-21888596 MS.gene39421:CDS 55.0%
GCTGTTGTTGCTGCTGCTCT+TGG + chr4.2:21888532-21888551 MS.gene39421:CDS 55.0%
GGAATGAGCGATAGTGACGG+AGG - chr4.2:21888017-21888036 None:intergenic 55.0%
TGGAGAGCTGAAGTTGGAGC+AGG - chr4.2:21887964-21887983 None:intergenic 55.0%
! GCAACAGCAGCCAAAAGCGT+CGG - chr4.2:21888463-21888482 None:intergenic 55.0%
! GTTGCTGCTGCTCTTGGTGT+TGG + chr4.2:21888538-21888557 MS.gene39421:CDS 55.0%
! TGCGGTCTCTCCTTCCGTTT+TGG + chr4.2:21888423-21888442 MS.gene39421:CDS 55.0%
!! CTCTTGGTGTTGGGGCTGTT+GGG + chr4.2:21888548-21888567 MS.gene39421:CDS 55.0%
!! GCTTTTGGCTGCTGTTGCGA+CGG + chr4.2:21888465-21888484 MS.gene39421:CDS 55.0%
!! TGCTGCTGCTCTTGGTGTTG+GGG + chr4.2:21888540-21888559 MS.gene39421:CDS 55.0%
TTTGGCTGCTGTTGCGACGG+TGG + chr4.2:21888468-21888487 MS.gene39421:CDS 60.0%
! AGCGATAGTGACGGAGGGTG+CGG - chr4.2:21888011-21888030 None:intergenic 60.0%
!! GCTCTTGGTGTTGGGGCTGT+TGG + chr4.2:21888547-21888566 MS.gene39421:CDS 60.0%
!! TGTTCTCGGCGGGTTGCAAG+AGG + chr4.2:21888588-21888607 MS.gene39421:CDS 60.0%
! GATAGTGACGGAGGGTGCGG+TGG - chr4.2:21888008-21888027 None:intergenic 65.0%
! GGAGGGTGCGGTGGTTAGTG+TGG - chr4.2:21887999-21888018 None:intergenic 65.0%
! GGGGCTGTTGGGTTGTTCGC+TGG + chr4.2:21888559-21888578 MS.gene39421:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 21887952 21888618 21887952 ID=MS.gene39421
chr4.2 mRNA 21887952 21888618 21887952 ID=MS.gene39421.t1;Parent=MS.gene39421
chr4.2 exon 21887952 21888089 21887952 ID=MS.gene39421.t1.exon1;Parent=MS.gene39421.t1
chr4.2 CDS 21887952 21888089 21887952 ID=cds.MS.gene39421.t1;Parent=MS.gene39421.t1
chr4.2 exon 21888175 21888618 21888175 ID=MS.gene39421.t1.exon2;Parent=MS.gene39421.t1
chr4.2 CDS 21888175 21888618 21888175 ID=cds.MS.gene39421.t1;Parent=MS.gene39421.t1
Gene Sequence

>MS.gene39421

ATGGCTGTTCCTGCTCCAACTTCAGCTCTCCATCTTCAACACATCCACACTAACCACCGCACCCTCCGTCACTATCGCTCATTCCCTACTTCCTCCAATAAACCTTTCACCGTCTTTGCCATGGCTCCCAAAAAGAAGGTACATACACAAAAATCCTCATCATTTTCGTAATTATTTTTTAATTTATACAATTTATTTGAATTTATGTTAATGGATATGGAAGGTGAACAAGTACAACGATAACTGGAAGAAACAATGGTTCGGAGCAGGAATATTCTACGAAGGAAGCGAAGAAGTAGAAGTGGACGTATTCAAGAAACTCGAGAAACGCAAAGTTTTAAGCAACGTTGAAAAAGCAGGATTATTATCCAAAGCTGAAGAATTCGGCGTCACGCTCTCTTCAATCGAGAAACTAGGACTTTTTTCTAAGGCGGAAGAACTTGGCTTACTCAGCTTATTAGAGAAACTCGCTGCGGTCTCTCCTTCCGTTTTGGCTTCTCTGTCACTTCCGACGCTTTTGGCTGCTGTTGCGACGGTGGTTCTTGTTCCTGATGATACTACCGCTCTTGTTGTAGTACAGGCTGTTGTTGCTGCTGCTCTTGGTGTTGGGGCTGTTGGGTTGTTCGCTGGATCTGTTGTTCTCGGCGGGTTGCAAGAGGCTGATTGA

Protein sequence

>MS.gene39421.t1

MAVPAPTSALHLQHIHTNHRTLRHYRSFPTSSNKPFTVFAMAPKKKVNKYNDNWKKQWFGAGIFYEGSEEVEVDVFKKLEKRKVLSNVEKAGLLSKAEEFGVTLSSIEKLGLFSKAEELGLLSLLEKLAAVSPSVLASLSLPTLLAAVATVVLVPDDTTALVVVQAVVAAALGVGAVGLFAGSVVLGGLQEAD