AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008379.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008379.01.T01 MTR_2g041260 95.000 120 5 1 1 119 1 120 2.88e-58 176
MsG0280008379.01.T01 MTR_4g125600 65.000 120 36 3 1 118 1 116 2.22e-43 138
MsG0280008379.01.T01 MTR_3g104950 56.557 122 46 4 1 118 1 119 1.81e-35 118
MsG0280008379.01.T01 MTR_1g015290 56.800 125 46 4 1 118 1 124 1.99e-35 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008379.01.T01 AT2G06520 55.645 124 40 4 1 118 1 115 6.47e-29 101

Find 41 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAATCCACTGTCTCTT+TGG 0.238913 2:+30320508 MsG0280008379.01.T01:CDS
TCGCTTGACGGGATCAAAAT+TGG 0.246839 2:-30320757 None:intergenic
CGATACAACTTTCTCCTTCA+TGG 0.258500 2:-30320586 None:intergenic
GTTGTGGTTGTAGTTATTAT+TGG 0.278706 2:+30320717 MsG0280008379.01.T01:CDS
AGCTGTTCACCACCCTCTTC+TGG 0.315003 2:-30320476 None:intergenic
GCAATGGAAGATTTCGAGTT+AGG 0.322324 2:+30320559 MsG0280008379.01.T01:CDS
TTCTTGCTCAGCATTGCAGC+TGG 0.329837 2:+30320690 MsG0280008379.01.T01:CDS
CAAAGATCCCGTAGCCTTTG+AGG 0.371295 2:-30320532 None:intergenic
CAATGGAAGATTTCGAGTTA+GGG 0.404333 2:+30320560 MsG0280008379.01.T01:CDS
TTCTGGCTGGTGTAAGTTAG+TGG 0.412688 2:-30320459 None:intergenic
ATTTATCAGCGTCGCTTGAC+GGG 0.428873 2:-30320768 None:intergenic
AATTTATCAGCGTCGCTTGA+CGG 0.443913 2:-30320769 None:intergenic
CTTGCTCAGCATTGCAGCTG+GGG 0.446840 2:+30320692 MsG0280008379.01.T01:CDS
GTTCACCACCCTCTTCTGGC+TGG 0.465649 2:-30320472 None:intergenic
TCTTGCTCAGCATTGCAGCT+GGG 0.491257 2:+30320691 MsG0280008379.01.T01:CDS
TCTTTGGTCCTCAAAGGCTA+CGG 0.494360 2:+30320524 MsG0280008379.01.T01:CDS
CTCCACTTCTGCAGTTTCAG+TGG 0.496724 2:+30320431 MsG0280008379.01.T01:CDS
TTCAATGGTGGTTCCTGATG+TGG 0.502456 2:+30320635 MsG0280008379.01.T01:CDS
GGCAGCATTGACAGCTTCAA+TGG 0.502656 2:+30320620 MsG0280008379.01.T01:CDS
TGCCACTGAAACTGCAGAAG+TGG 0.518999 2:-30320433 None:intergenic
CATGAAGGAGAAAGTTGTAT+CGG 0.528319 2:+30320587 MsG0280008379.01.T01:CDS
TCGAGTTAGGGCTTCCATGA+AGG 0.531531 2:+30320572 MsG0280008379.01.T01:CDS
CTTTGGTCCTCAAAGGCTAC+GGG 0.540705 2:+30320525 MsG0280008379.01.T01:CDS
TTGCTCAGCATTGCAGCTGG+GGG 0.544179 2:+30320693 MsG0280008379.01.T01:CDS
AGCAAGAAGTTCTTGAGAGA+AGG 0.544406 2:-30320675 None:intergenic
ACGGCCGCTTCAGCCACATC+AGG 0.547544 2:-30320648 None:intergenic
ATGAAGGAGAAAGTTGTATC+GGG 0.551364 2:+30320588 MsG0280008379.01.T01:CDS
ACTGTCTCTTTGGTCCTCAA+AGG 0.567613 2:+30320518 MsG0280008379.01.T01:CDS
CACTGAAACTGCAGAAGTGG+AGG 0.579775 2:-30320430 None:intergenic
ACGGGATCTTTGAAACGCAA+TGG 0.585660 2:+30320543 MsG0280008379.01.T01:CDS
GGTTCCTGATGTGGCTGAAG+CGG 0.586157 2:+30320644 MsG0280008379.01.T01:CDS
GTTCTTGAGAGAAGGTGAAA+CGG 0.587992 2:-30320667 None:intergenic
AACTTACACCAGCCAGAAGA+GGG 0.588425 2:+30320464 MsG0280008379.01.T01:CDS
TAACTTACACCAGCCAGAAG+AGG 0.600752 2:+30320463 MsG0280008379.01.T01:CDS
CAGTGGATTGAAGAATGCCT+CGG 0.611133 2:-30320499 None:intergenic
AGCATTGACAGCTTCAATGG+TGG 0.628440 2:+30320623 MsG0280008379.01.T01:CDS
TTTGAGGACCAAAGAGACAG+TGG 0.646236 2:-30320516 None:intergenic
GGTTGTAGTTATTATTGGTG+CGG 0.673405 2:+30320722 MsG0280008379.01.T01:CDS
AGTTGTATCGGGACTCACAG+CGG 0.708081 2:+30320599 MsG0280008379.01.T01:CDS
TTACACCAGCCAGAAGAGGG+TGG 0.718271 2:+30320467 MsG0280008379.01.T01:CDS
GAGGGTGGTGAACAGCTCCG+AGG 0.735900 2:+30320482 MsG0280008379.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTTGTGGTTGTAGTTATTAT+TGG + Chr2:30320717-30320736 MsG0280008379.01.T01:CDS 30.0%
ATGAAGGAGAAAGTTGTATC+GGG + Chr2:30320588-30320607 MsG0280008379.01.T01:CDS 35.0%
CATGAAGGAGAAAGTTGTAT+CGG + Chr2:30320587-30320606 MsG0280008379.01.T01:CDS 35.0%
GGTTGTAGTTATTATTGGTG+CGG + Chr2:30320722-30320741 MsG0280008379.01.T01:CDS 35.0%
!! CAATGGAAGATTTCGAGTTA+GGG + Chr2:30320560-30320579 MsG0280008379.01.T01:CDS 35.0%
AGCAAGAAGTTCTTGAGAGA+AGG - Chr2:30320678-30320697 None:intergenic 40.0%
CGATACAACTTTCTCCTTCA+TGG - Chr2:30320589-30320608 None:intergenic 40.0%
GTTCTTGAGAGAAGGTGAAA+CGG - Chr2:30320670-30320689 None:intergenic 40.0%
TCTTCAATCCACTGTCTCTT+TGG + Chr2:30320508-30320527 MsG0280008379.01.T01:CDS 40.0%
!! GCAATGGAAGATTTCGAGTT+AGG + Chr2:30320559-30320578 MsG0280008379.01.T01:CDS 40.0%
AACTTACACCAGCCAGAAGA+GGG + Chr2:30320464-30320483 MsG0280008379.01.T01:CDS 45.0%
ACTGTCTCTTTGGTCCTCAA+AGG + Chr2:30320518-30320537 MsG0280008379.01.T01:CDS 45.0%
TAACTTACACCAGCCAGAAG+AGG + Chr2:30320463-30320482 MsG0280008379.01.T01:CDS 45.0%
TCGCTTGACGGGATCAAAAT+TGG - Chr2:30320760-30320779 None:intergenic 45.0%
TCTTTGGTCCTCAAAGGCTA+CGG + Chr2:30320524-30320543 MsG0280008379.01.T01:CDS 45.0%
TTCAATGGTGGTTCCTGATG+TGG + Chr2:30320635-30320654 MsG0280008379.01.T01:CDS 45.0%
TTTGAGGACCAAAGAGACAG+TGG - Chr2:30320519-30320538 None:intergenic 45.0%
! TTCTGGCTGGTGTAAGTTAG+TGG - Chr2:30320462-30320481 None:intergenic 45.0%
!! ACGGGATCTTTGAAACGCAA+TGG + Chr2:30320543-30320562 MsG0280008379.01.T01:CDS 45.0%
!! AGCATTGACAGCTTCAATGG+TGG + Chr2:30320623-30320642 MsG0280008379.01.T01:CDS 45.0%
!! CAGTGGATTGAAGAATGCCT+CGG - Chr2:30320502-30320521 None:intergenic 45.0%
AGTTGTATCGGGACTCACAG+CGG + Chr2:30320599-30320618 MsG0280008379.01.T01:CDS 50.0%
CAAAGATCCCGTAGCCTTTG+AGG - Chr2:30320535-30320554 None:intergenic 50.0%
CACTGAAACTGCAGAAGTGG+AGG - Chr2:30320433-30320452 None:intergenic 50.0%
CTCCACTTCTGCAGTTTCAG+TGG + Chr2:30320431-30320450 MsG0280008379.01.T01:CDS 50.0%
CTTTGGTCCTCAAAGGCTAC+GGG + Chr2:30320525-30320544 MsG0280008379.01.T01:CDS 50.0%
TCGAGTTAGGGCTTCCATGA+AGG + Chr2:30320572-30320591 MsG0280008379.01.T01:CDS 50.0%
TCTTGCTCAGCATTGCAGCT+GGG + Chr2:30320691-30320710 MsG0280008379.01.T01:CDS 50.0%
TGCCACTGAAACTGCAGAAG+TGG - Chr2:30320436-30320455 None:intergenic 50.0%
TTCTTGCTCAGCATTGCAGC+TGG + Chr2:30320690-30320709 MsG0280008379.01.T01:CDS 50.0%
!! GGCAGCATTGACAGCTTCAA+TGG + Chr2:30320620-30320639 MsG0280008379.01.T01:CDS 50.0%
AGCTGTTCACCACCCTCTTC+TGG - Chr2:30320479-30320498 None:intergenic 55.0%
CTTGCTCAGCATTGCAGCTG+GGG + Chr2:30320692-30320711 MsG0280008379.01.T01:CDS 55.0%
GGTTCCTGATGTGGCTGAAG+CGG + Chr2:30320644-30320663 MsG0280008379.01.T01:CDS 55.0%
TTACACCAGCCAGAAGAGGG+TGG + Chr2:30320467-30320486 MsG0280008379.01.T01:CDS 55.0%
TTGCTCAGCATTGCAGCTGG+GGG + Chr2:30320693-30320712 MsG0280008379.01.T01:CDS 55.0%
CATTGCAGCTGGGGGAGTTG+TGG + Chr2:30320701-30320720 MsG0280008379.01.T01:CDS 60.0%
GTTCACCACCCTCTTCTGGC+TGG - Chr2:30320475-30320494 None:intergenic 60.0%
ACGGCCGCTTCAGCCACATC+AGG - Chr2:30320651-30320670 None:intergenic 65.0%
GAGGGTGGTGAACAGCTCCG+AGG + Chr2:30320482-30320501 MsG0280008379.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 30320426 30320785 30320426 ID=MsG0280008379.01;Name=MsG0280008379.01
Chr2 mRNA 30320426 30320785 30320426 ID=MsG0280008379.01.T01;Parent=MsG0280008379.01;Name=MsG0280008379.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|119
Chr2 exon 30320426 30320785 30320426 ID=MsG0280008379.01.T01:exon:5762;Parent=MsG0280008379.01.T01
Chr2 CDS 30320426 30320785 30320426 ID=MsG0280008379.01.T01:cds;Parent=MsG0280008379.01.T01
Gene Sequence

>MsG0280008379.01.T01

ATGGCCTCCACTTCTGCAGTTTCAGTGGCAATGCCACTAACTTACACCAGCCAGAAGAGGGTGGTGAACAGCTCCGAGGCATTCTTCAATCCACTGTCTCTTTGGTCCTCAAAGGCTACGGGATCTTTGAAACGCAATGGAAGATTTCGAGTTAGGGCTTCCATGAAGGAGAAAGTTGTATCGGGACTCACAGCGGCAGCATTGACAGCTTCAATGGTGGTTCCTGATGTGGCTGAAGCGGCCGTTTCACCTTCTCTCAAGAACTTCTTGCTCAGCATTGCAGCTGGGGGAGTTGTGGTTGTAGTTATTATTGGTGCGGTGATCGCTGTATCCAATTTTGATCCCGTCAAGCGACGCTGA

Protein sequence

>MsG0280008379.01.T01

MASTSAVSVAMPLTYTSQKRVVNSSEAFFNPLSLWSSKATGSLKRNGRFRVRASMKEKVVSGLTAAALTASMVVPDVAEAAVSPSLKNFLLSIAAGGVVVVVIIGAVIAVSNFDPVKRR*