Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012780.01.T01 | XP_003599258.1 | 93.333 | 105 | 7 | 0 | 1 | 105 | 1 | 105 | 3.04E-53 | 172 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0380012780.01 | 0.858620 | 7.269576e-63 | 1.866703e-59 |
| MsG0080047945.01 | MsG0380012780.01 | 0.902318 | 1.095366e-78 | 1.542122e-74 |
| MsG0080047955.01 | MsG0380012780.01 | 0.929884 | 3.658994e-93 | 2.094685e-88 |
| MsG0080047956.01 | MsG0380012780.01 | 0.934756 | 2.466197e-96 | 1.905648e-91 |
| MsG0080047989.01 | MsG0380012780.01 | 0.800239 | 1.582445e-48 | 7.330976e-46 |
| MsG0080048059.01 | MsG0380012780.01 | 0.860609 | 1.833600e-63 | 5.041685e-60 |
| MsG0080048068.01 | MsG0380012780.01 | 0.814241 | 1.702772e-51 | 1.136482e-48 |
| MsG0080048528.01 | MsG0380012780.01 | 0.810670 | 1.026895e-50 | 6.228683e-48 |
| MsG0080048611.01 | MsG0380012780.01 | 0.856485 | 3.116922e-62 | 7.452054e-59 |
| MsG0080048776.01 | MsG0380012780.01 | 0.809980 | 1.446688e-50 | 8.616756e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012780.01.T01 | MTR_3g030860 | 93.333 | 105 | 7 | 0 | 1 | 105 | 1 | 105 | 3.68e-57 | 172 |
| MsG0380012780.01.T01 | MTR_3g030850 | 80.000 | 105 | 20 | 1 | 1 | 104 | 1 | 105 | 1.08e-51 | 158 |
| MsG0380012780.01.T01 | MTR_4g037505 | 67.593 | 108 | 32 | 3 | 1 | 105 | 1 | 108 | 7.68e-39 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012780.01.T01 | AT1G51400 | 54.206 | 107 | 44 | 3 | 1 | 104 | 1 | 105 | 1.27e-27 | 97.4 |
| MsG0380012780.01.T01 | AT3G21055 | 53.271 | 107 | 44 | 4 | 1 | 105 | 1 | 103 | 1.59e-23 | 87.0 |
| MsG0380012780.01.T01 | AT3G21055 | 53.271 | 107 | 44 | 4 | 1 | 105 | 41 | 143 | 2.71e-23 | 87.8 |
Find 32 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGACAAGGAGGGTAGCAA+TGG | 0.572603 | 3:-24443175 | MsG0380012780.01.T01:CDS |
| ACAAGGAGGGTAGCAATGGA+AGG | 0.594273 | 3:-24443171 | MsG0380012780.01.T01:CDS |
| ACCAACTATTGAGGATGTCA+TGG | 0.574051 | 3:+24443297 | None:intergenic |
| ACCAAGAAGAGGCACCCCAG+AGG | 0.700077 | 3:-24443077 | MsG0380012780.01.T01:CDS |
| ACCATGACATCCTCAATAGT+TGG | 0.463538 | 3:-24443298 | MsG0380012780.01.T01:CDS |
| AGCAGTTGAAGGGGAAAAGA+CGG | 0.619574 | 3:-24443209 | MsG0380012780.01.T01:CDS |
| AGCGACAAATCCTAGCAGTT+GGG | 0.546386 | 3:+24443020 | None:intergenic |
| AGTGCTGTAAGAGCAGTTGA+AGG | 0.554404 | 3:-24443220 | MsG0380012780.01.T01:CDS |
| AGTTGTACCAATGACAAGGA+GGG | 0.697533 | 3:-24443184 | MsG0380012780.01.T01:CDS |
| ATGACCGGTTGGTTATGGCA+GGG | 0.676181 | 3:+24443272 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCAACTATTGAGGATGTCA+TGG | + | Chr3:24443031-24443050 | None:intergenic | 40.0% | |
| ACCATGACATCCTCAATAGT+TGG | - | Chr3:24443027-24443046 | MsG0380012780.01.T01:CDS | 40.0% | |
| AGTTGTACCAATGACAAGGA+GGG | - | Chr3:24443141-24443160 | MsG0380012780.01.T01:CDS | 40.0% | |
| AATGACAAGGAGGGTAGCAA+TGG | - | Chr3:24443150-24443169 | MsG0380012780.01.T01:CDS | 45.0% | |
| AGCAGTTGAAGGGGAAAAGA+CGG | - | Chr3:24443116-24443135 | MsG0380012780.01.T01:CDS | 45.0% | |
| AGCGACAAATCCTAGCAGTT+GGG | + | Chr3:24443308-24443327 | None:intergenic | 45.0% | |
| AGTGCTGTAAGAGCAGTTGA+AGG | - | Chr3:24443105-24443124 | MsG0380012780.01.T01:CDS | 45.0% | |
| ATTGCTACCCTCCTTGTCAT+TGG | + | Chr3:24443151-24443170 | None:intergenic | 45.0% | |
| CAGTTGTACCAATGACAAGG+AGG | - | Chr3:24443140-24443159 | MsG0380012780.01.T01:CDS | 45.0% | |
| CCAGAGGCCAAGAAAAAGTA+TGG | - | Chr3:24443264-24443283 | MsG0380012780.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 24443015 | 24443332 | 24443015 | ID=MsG0380012780.01;Name=MsG0380012780.01 |
| Chr3 | mRNA | 24443015 | 24443332 | 24443015 | ID=MsG0380012780.01.T01;Parent=MsG0380012780.01;Name=MsG0380012780.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|105 |
| Chr3 | exon | 24443015 | 24443332 | 24443015 | ID=MsG0380012780.01.T01:exon:8842;Parent=MsG0380012780.01.T01 |
| Chr3 | CDS | 24443015 | 24443332 | 24443015 | ID=MsG0380012780.01.T01:cds;Parent=MsG0380012780.01.T01 |
| Gene Sequence |
>MsG0380012780.01.T01 ATGACATCAATCACCATGACATCCTCAATAGTTGGTTTCCCTGCCATAACCAACCGGTCATCAGTAGCAACACAGCGAAGATTTGTTGTCAGTGCTGTAAGAGCAGTTGAAGGGGAAAAGACGGTCAGTTGTACCAATGACAAGGAGGGTAGCAATGGAAGGAGAGATTTGATGTTTGCTGCAGCAGCAGCTGCTGTTTGCTCTGTTGCTGGGGTTGCAATGGCAGATGATGAACCAAGAAGAGGCACCCCAGAGGCCAAGAAAAAGTATGGCCCTGTTTGTGTCACTAACCCAACTGCTAGGATTTGTCGCTATTGA |
| Protein sequence |
>MsG0380012780.01.T01 MTSITMTSSIVGFPAITNRSSVATQRRFVVSAVRAVEGEKTVSCTNDKEGSNGRRDLMFAAAAAAVCSVAGVAMADDEPRRGTPEAKKKYGPVCVTNPTARICRY* |
