Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034419.01.T01 | QMS43324.1 | 99.415 | 171 | 1 | 0 | 1 | 171 | 1 | 171 | 6.42E-111 | 342 |
| MsG0680034419.01.T02 | PNY13156.1 | 98.089 | 157 | 3 | 0 | 1 | 157 | 113 | 269 | 4.72E-102 | 310 |
| MsG0680034419.01.T03 | QMS43324.1 | 95.918 | 147 | 6 | 0 | 3 | 149 | 220 | 366 | 5.76E-93 | 293 |
| MsG0680034419.01.T04 | QMS43324.1 | 98.551 | 138 | 2 | 0 | 23 | 160 | 549 | 686 | 2.69E-91 | 290 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034419.01.T01 | Q8RXN0 | 75.568 | 176 | 34 | 3 | 1 | 170 | 1 | 173 | 4.44E-82 | 259 |
| MsG0680034419.01.T02 | Q8RXN0 | 90.541 | 148 | 14 | 0 | 1 | 148 | 395 | 542 | 1.30E-90 | 281 |
| MsG0680034419.01.T03 | Q8RXN0 | 84.354 | 147 | 23 | 0 | 3 | 149 | 223 | 369 | 1.63E-83 | 261 |
| MsG0680034419.01.T04 | Q8RXN0 | 79.412 | 136 | 27 | 1 | 25 | 160 | 554 | 688 | 4.60E-61 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034419.01.T01 | A0A072UC92 | 99.415 | 171 | 1 | 0 | 1 | 171 | 1 | 171 | 3.07e-111 | 342 |
| MsG0680034419.01.T02 | A0A2K3PCY4 | 98.089 | 157 | 3 | 0 | 1 | 157 | 113 | 269 | 2.26e-102 | 310 |
| MsG0680034419.01.T03 | A0A7D7KGP8 | 95.918 | 147 | 6 | 0 | 3 | 149 | 220 | 366 | 2.75e-93 | 293 |
| MsG0680034419.01.T04 | A0A7D7KGP8 | 98.551 | 138 | 2 | 0 | 23 | 160 | 549 | 686 | 1.29e-91 | 290 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0680034419.01 | 0.878230 | 3.321369e-69 | 1.720926e-65 |
| MsG0080047945.01 | MsG0680034419.01 | 0.853936 | 1.718863e-61 | 3.775859e-58 |
| MsG0080047955.01 | MsG0680034419.01 | 0.838436 | 2.878864e-57 | 3.855891e-54 |
| MsG0080047956.01 | MsG0680034419.01 | 0.850372 | 1.771535e-60 | 3.462543e-57 |
| MsG0080048059.01 | MsG0680034419.01 | 0.896187 | 4.692080e-76 | 5.020785e-72 |
| MsG0080048068.01 | MsG0680034419.01 | 0.821317 | 4.306614e-53 | 3.491803e-50 |
| MsG0080048198.01 | MsG0680034419.01 | 0.831330 | 1.774487e-55 | 1.919421e-52 |
| MsG0080048528.01 | MsG0680034419.01 | 0.851240 | 1.009390e-60 | 2.030090e-57 |
| MsG0080048611.01 | MsG0680034419.01 | 0.818881 | 1.555205e-52 | 1.178495e-49 |
| MsG0080048776.01 | MsG0680034419.01 | 0.841477 | 4.641397e-58 | 6.828726e-55 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034419.01.T01 | MTR_6g066240 | 99.415 | 171 | 1 | 0 | 1 | 171 | 1 | 171 | 7.78e-115 | 342 |
| MsG0680034419.01.T01 | MTR_4g094010 | 57.143 | 126 | 51 | 1 | 47 | 169 | 28 | 153 | 1.09e-39 | 143 |
| MsG0680034419.01.T01 | MTR_4g076970 | 54.762 | 126 | 56 | 1 | 46 | 170 | 30 | 155 | 2.27e-39 | 142 |
| MsG0680034419.01.T01 | MTR_4g094060 | 50.694 | 144 | 65 | 3 | 28 | 169 | 37 | 176 | 4.24e-39 | 141 |
| MsG0680034419.01.T01 | MTR_4g094050 | 50.694 | 144 | 65 | 3 | 28 | 169 | 37 | 176 | 1.97e-38 | 139 |
| MsG0680034419.01.T01 | MTR_4g076940 | 53.600 | 125 | 57 | 1 | 47 | 170 | 73 | 197 | 3.02e-37 | 136 |
| MsG0680034419.01.T01 | MTR_4g076940 | 53.600 | 125 | 57 | 1 | 47 | 170 | 33 | 157 | 3.07e-37 | 136 |
| MsG0680034419.01.T01 | MTR_4g076900 | 51.200 | 125 | 60 | 1 | 47 | 170 | 9 | 133 | 1.19e-35 | 132 |
| MsG0680034419.01.T01 | MTR_4g094090 | 51.613 | 124 | 57 | 2 | 47 | 169 | 13 | 134 | 1.05e-34 | 129 |
| MsG0680034419.01.T01 | MTR_4g093845 | 51.667 | 120 | 55 | 2 | 44 | 162 | 38 | 155 | 3.25e-33 | 124 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034419.01.T01 | AT1G17840 | 75.568 | 176 | 34 | 3 | 1 | 170 | 1 | 173 | 4.53e-83 | 259 |
| MsG0680034419.01.T01 | AT3G21090 | 57.480 | 127 | 53 | 1 | 45 | 170 | 23 | 149 | 4.42e-41 | 147 |
| MsG0680034419.01.T01 | AT1G51500 | 52.143 | 140 | 65 | 2 | 32 | 170 | 10 | 148 | 9.91e-39 | 140 |
| MsG0680034419.01.T01 | AT1G51460 | 54.839 | 124 | 55 | 1 | 47 | 169 | 10 | 133 | 2.57e-38 | 139 |
| MsG0680034419.01.T01 | AT2G28070 | 43.307 | 127 | 70 | 1 | 45 | 171 | 112 | 236 | 2.40e-25 | 102 |
| MsG0680034419.01.T01 | AT2G28070 | 43.307 | 127 | 70 | 1 | 45 | 171 | 112 | 236 | 2.40e-25 | 102 |
| MsG0680034419.01.T01 | AT1G53390 | 42.029 | 138 | 74 | 4 | 47 | 182 | 507 | 640 | 1.54e-23 | 97.4 |
| MsG0680034419.01.T01 | AT5G60740 | 37.600 | 125 | 72 | 3 | 47 | 169 | 499 | 619 | 3.48e-20 | 87.8 |
| MsG0680034419.01.T01 | AT3G55100 | 41.481 | 135 | 66 | 3 | 47 | 169 | 22 | 155 | 3.74e-20 | 87.8 |
| MsG0680034419.01.T01 | AT3G55100 | 41.481 | 135 | 66 | 3 | 47 | 169 | 22 | 155 | 3.74e-20 | 87.8 |
Find 40 sgRNAs with CRISPR-Local
Find 504 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACGTAATGAGAAAATCC+GGG | 0.596961 | 6:+87324873 | None:intergenic |
| AAACACTACGTGCATTGGCA+AGG | 0.548865 | 6:-87328163 | MsG0680034419.01.T01:three_prime_UTR |
| AAAGTGATTAATATGAGGAA+TGG | 0.552183 | 6:+87324936 | None:intergenic |
| AACACTACGTGCATTGGCAA+GGG | 0.583782 | 6:-87328162 | MsG0680034419.01.T01:three_prime_UTR |
| AACTGTGACACTTGCATAAA+GGG | 0.357856 | 6:+87324839 | None:intergenic |
| AATGAGAAAATCCGGGATGT+AGG | 0.547951 | 6:+87324880 | None:intergenic |
| AGAGAAAAGTGATTAATATG+AGG | 0.523389 | 6:+87324931 | None:intergenic |
| AGCTCAAACACTTCACTGCT+AGG | 0.538912 | 6:+87328107 | None:intergenic |
| AGTCAAAGTGGACAATACTT+TGG | 0.496882 | 6:-87324549 | MsG0680034419.01.T01:intron |
| AGTTGTTGAAAGCTTAATGA+TGG | 0.487154 | 6:-87324820 | MsG0680034419.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACGTAATGAGAAAATCC+GGG | + | Chr6:87330407-87330426 | None:intergenic | 30.0% | |
| AAAGCAAAACCAGATATCAT+TGG | - | Chr6:87323740-87323759 | MsG0680034419.01.T01:intron | 30.0% | |
| AAATAACATACTTGTAGGGT+TGG | - | Chr6:87326428-87326447 | MsG0680034419.01.T01:intron | 30.0% | |
| AAGAAGAGTTAGTATTGCAT+TGG | - | Chr6:87325548-87325567 | MsG0680034419.01.T01:intron | 30.0% | |
| AAGACTTGAATTGAAAATCC+TGG | - | Chr6:87326791-87326810 | MsG0680034419.01.T01:intron | 30.0% | |
| AAGTCTTCATTCAAACTTAG+AGG | + | Chr6:87332059-87332078 | None:intergenic | 30.0% | |
| AATGATACATCTATGAAGCA+CGG | - | Chr6:87328016-87328035 | MsG0680034419.01.T01:intron | 30.0% | |
| ACAACATTAAAACTCATGTC+TGG | + | Chr6:87324731-87324750 | None:intergenic | 30.0% | |
| ACACAAGATGACAATTTGAT+TGG | - | Chr6:87325349-87325368 | MsG0680034419.01.T01:intron | 30.0% | |
| ACTTCATTTCCTAATGAACT+TGG | + | Chr6:87323668-87323687 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 87323096 | 87332203 | 87323096 | ID=MsG0680034419.01;Name=MsG0680034419.01 |
| Chr6 | mRNA | 87323096 | 87332203 | 87323096 | ID=MsG0680034419.01.T01;Parent=MsG0680034419.01;Name=MsG0680034419.01.T01;_AED=0.33;_eAED=0.33;_QI=545|1|1|1|0.66|0.8|10|1841|189 |
| Chr6 | exon | 87324762 | 87325033 | 87324762 | ID=MsG0680034419.01.T01:exon:7439;Parent=MsG0680034419.01.T01 |
| Chr6 | five_prime_UTR | 87331659 | 87332203 | 87331659 | ID=MsG0680034419.01.T01:five_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | CDS | 87331284 | 87331658 | 87331284 | ID=MsG0680034419.01.T01:cds;Parent=MsG0680034419.01.T01 |
| Chr6 | CDS | 87329765 | 87329959 | 87329765 | ID=MsG0680034419.01.T01:cds;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87329671 | 87329764 | 87329671 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87328037 | 87328208 | 87328037 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87327571 | 87327693 | 87327571 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87326811 | 87326939 | 87326811 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87325846 | 87326039 | 87325846 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87325467 | 87325553 | 87325467 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87324762 | 87325033 | 87324762 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87323922 | 87324029 | 87323922 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | three_prime_UTR | 87323096 | 87323757 | 87323096 | ID=MsG0680034419.01.T01:three_prime_utr;Parent=MsG0680034419.01.T01 |
| Chr6 | mRNA | 87324698 | 87332203 | 87324698 | ID=MsG0680034419.01.T02;Parent=MsG0680034419.01;Name=MsG0680034419.01.T02;_AED=0.37;_eAED=0.37;_QI=1716|1|0.87|1|0.71|0.75|8|0|177 |
| Chr6 | exon | 87324698 | 87325033 | 87324698 | ID=MsG0680034419.01.T02:exon:7442;Parent=MsG0680034419.01.T02 |
| Chr6 | five_prime_UTR | 87331284 | 87332203 | 87331284 | ID=MsG0680034419.01.T02:five_prime_utr;Parent=MsG0680034419.01.T02 |
| Chr6 | five_prime_UTR | 87329671 | 87329959 | 87329671 | ID=MsG0680034419.01.T02:five_prime_utr;Parent=MsG0680034419.01.T02 |
| Chr6 | five_prime_UTR | 87328037 | 87328208 | 87328037 | ID=MsG0680034419.01.T02:five_prime_utr;Parent=MsG0680034419.01.T02 |
| Chr6 | five_prime_UTR | 87327571 | 87327693 | 87327571 | ID=MsG0680034419.01.T02:five_prime_utr;Parent=MsG0680034419.01.T02 |
| Chr6 | five_prime_UTR | 87326811 | 87326939 | 87326811 | ID=MsG0680034419.01.T02:five_prime_utr;Parent=MsG0680034419.01.T02 |
| Chr6 | five_prime_UTR | 87325957 | 87326039 | 87325957 | ID=MsG0680034419.01.T02:five_prime_utr;Parent=MsG0680034419.01.T02 |
| Chr6 | CDS | 87325846 | 87325956 | 87325846 | ID=MsG0680034419.01.T02:cds;Parent=MsG0680034419.01.T02 |
| Chr6 | CDS | 87325467 | 87325553 | 87325467 | ID=MsG0680034419.01.T02:cds;Parent=MsG0680034419.01.T02 |
| Chr6 | CDS | 87324698 | 87325033 | 87324698 | ID=MsG0680034419.01.T02:cds;Parent=MsG0680034419.01.T02 |
| Chr6 | mRNA | 87326790 | 87328222 | 87326790 | ID=MsG0680034419.01.T03;Parent=MsG0680034419.01;Name=MsG0680034419.01.T03;_AED=0.47;_eAED=0.49;_QI=0|1|0.33|1|1|1|3|0|152 |
| Chr6 | exon | 87328037 | 87328222 | 87328037 | ID=MsG0680034419.01.T03:exon:7444;Parent=MsG0680034419.01.T03 |
| Chr6 | exon | 87326790 | 87326939 | 87326790 | ID=MsG0680034419.01.T03:exon:7443;Parent=MsG0680034419.01.T03 |
| Chr6 | CDS | 87328037 | 87328222 | 87328037 | ID=MsG0680034419.01.T03:cds;Parent=MsG0680034419.01.T03 |
| Chr6 | CDS | 87327571 | 87327693 | 87327571 | ID=MsG0680034419.01.T03:cds;Parent=MsG0680034419.01.T03 |
| Chr6 | CDS | 87326790 | 87326939 | 87326790 | ID=MsG0680034419.01.T03:cds;Parent=MsG0680034419.01.T03 |
| Chr6 | mRNA | 87323096 | 87324615 | 87323096 | ID=MsG0680034419.01.T04;Parent=MsG0680034419.01;Name=MsG0680034419.01.T04;_AED=0.50;_eAED=0.50;_QI=0|0.5|0.66|0.66|1|0.66|3|353|160 |
| Chr6 | exon | 87324550 | 87324615 | 87324550 | ID=MsG0680034419.01.T04:exon:7445;Parent=MsG0680034419.01.T04 |
| Chr6 | CDS | 87324550 | 87324615 | 87324550 | ID=MsG0680034419.01.T04:cds;Parent=MsG0680034419.01.T04 |
| Chr6 | CDS | 87323922 | 87324029 | 87323922 | ID=MsG0680034419.01.T04:cds;Parent=MsG0680034419.01.T04 |
| Chr6 | CDS | 87323449 | 87323757 | 87323449 | ID=MsG0680034419.01.T04:cds;Parent=MsG0680034419.01.T04 |
| Chr6 | three_prime_UTR | 87323096 | 87323448 | 87323096 | ID=MsG0680034419.01.T04:three_prime_utr;Parent=MsG0680034419.01.T04 |
| Gene Sequence |
>MsG0680034419.01.T01 ATGGAGATAGAACAAAGTTACAAACCAAGTTCATTAGGAAATGAAGTACCATCAAATTTACCATCTTTGAGTCCATTGAGTGAAACCCTATGGAGAGAAAAAGCAAAACCAGATATCATTGGTGATGTTTCAGCAAGATTAACATGGAAAGATTTAACTGTTATGGTAACCCTAAGTAATGGTGAAATACAAAATGTTCTTGAGAATTTAACTGGTTATGCTGAACCAGGTTATTTCACTGCTCTTATGGGACCTTCTGGTTCTGGAAAATCAACTCTTCTTGATGCACTTTCTAGTCGTTTAGCTTCTAATGCTTTTCTTTCTGGTACTATTCTTCTCAATGGTCGCAAAGAAAAGTTATCTTTTGGAACTGCTGCCTATGTGACACAAGATGACAATTTGATTGGCACCTTAACAGTAAGAGAAACAATATGGTACTCAGCTAGGTTAAGATTACCAGATAAAATGTCAAGATCAGATAAAAGAGCATTAGTTGAAAGCACAATTGTTGCATGGGTCTTCAAGATTGTGCCGACACAGTTATTGGTAATTGGCATTTACGTGGGATAA >MsG0680034419.01.T03 ATGATTTTATGCAGTGCTTCAGCTTTCTTTGTGACTCAAACACTACGTGCATTGGCAAGGGATGGAAGAACAGTGATAGCTTCTATTCATCAACCTAGCAGTGAAGTGTTTGAGCTATTTGACCAATTGTATTTGCTTTCTGGTGGCAAAACTGTTTATTTTGGCCAAGCCTCAGATGCATATGAGTTTTTTGCACAAGCTGGATTTCCATGCCCTGCTTTGAGGAACCCTTCTGATCATTTTCTTAGATGCATCAATTCTGACTTTGATAAGGTCCAAGCCACTCTTAAAGGGTCCATGAAATTGAGGTTTGAGGCAAGTGATGACCCTCTAGACAAGATCTCTACTGCTGAAGCAATCAGAACACTTATTGAACACTACCGGACTTCTCAACACTCTTATGCTGCAAGACAAAAAGTTGATGACATATCCAAAGTTGTAAGTACTTTTCAATTATAA >MsG0680034419.01.T04 ATGAGGCGTCTTTTACAAAAGACAAACCGCGAGGCTTACACAAAAGTCAAAGTGGACAATACTTTGGGTATCTTCATGTTGGTCTCTGGCTACTTTAGGCTCCCACATGATATCCCAAAGCCTGTTTGGCGTTATCCAATGTCATACATCAGTTTCCACTATTGGGCATTACAGGGCCAATACCAGAACGATCTTAGGGGTTTGTTATTCGACGGTGAAACACCTGATGCGCCAAAGATAGCTGGCGAATACATCTTGGAGTACGCATTCCAAATCACTGTGACCAGATCAAAATGGATAGATTTATCGGTCATCTTAAGCATGATTATCATATATCGCATTATCTTCTTCGTCATGATCAAGGTCAATGAGGATGTTACTCCATGGGTTAGAGGGTATCTTGCTAGGAGAAGAATCCAACAAAAGAGTGGAGCTCAGAATACAACTATTGCACCTGATGTTCTTACCCAGTCTCCATCTTGA >MsG0680034419.01.T02 ATGTCAAGGGACTTTGGTTACTATTGGCTTAGGCTTGTAATCTACATTGTTGTCACTATCTGCATTGGAACCATTTACTTGAATGTTGGAACTGGCTACAACTCAATTCTGGCTAGGGGTTCATGTGCATCTTTTGTGTTTGGATTTGTCACCTTCATGTCAATTGGTGGATTCCCTTCGTTTGTTGAAGATATGAAGGTTTTTCAAAGGGAGAGGCTAAATGGACACTATGGTGTGACTGCATTTGTTATTAGCAATACATTATCTGCAACACCATTCCTCATATTAATCACTTTTCTCTCTGGAACAATTTGTTACTTCATGGTTAACCTACATCCCGGATTTTCTCATTACGTTTTCTTTGTGCTGGCCCTTTATGCAAGTGTCACAGTTGTTGAAAGCTTAATGATGGCAATTGCTAGTATTGTTCCTAACTTCCTCATGGGCATTATCATTGGAGCAGGAATCAAGTATGCACCTAACTTTAATCAGCTTACAAATTTCCATATTTTGTTTAAATATATAAATTTCTAG |
| Protein sequence |
>MsG0680034419.01.T01 MEIEQSYKPSSLGNEVPSNLPSLSPLSETLWREKAKPDIIGDVSARLTWKDLTVMVTLSNGEIQNVLENLTGYAEPGYFTALMGPSGSGKSTLLDALSSRLASNAFLSGTILLNGRKEKLSFGTAAYVTQDDNLIGTLTVRETIWYSARLRLPDKMSRSDKRALVESTIVAWVFKIVPTQLLVIGIYVG* >MsG0680034419.01.T03 MILCSASAFFVTQTLRALARDGRTVIASIHQPSSEVFELFDQLYLLSGGKTVYFGQASDAYEFFAQAGFPCPALRNPSDHFLRCINSDFDKVQATLKGSMKLRFEASDDPLDKISTAEAIRTLIEHYRTSQHSYAARQKVDDISKVVSTFQL* >MsG0680034419.01.T04 MRRLLQKTNREAYTKVKVDNTLGIFMLVSGYFRLPHDIPKPVWRYPMSYISFHYWALQGQYQNDLRGLLFDGETPDAPKIAGEYILEYAFQITVTRSKWIDLSVILSMIIIYRIIFFVMIKVNEDVTPWVRGYLARRRIQQKSGAQNTTIAPDVLTQSPS* >MsG0680034419.01.T02 MSRDFGYYWLRLVIYIVVTICIGTIYLNVGTGYNSILARGSCASFVFGFVTFMSIGGFPSFVEDMKVFQRERLNGHYGVTAFVISNTLSATPFLILITFLSGTICYFMVNLHPGFSHYVFFVLALYASVTVVESLMMAIASIVPNFLMGIIIGAGIKYAPNFNQLTNFHILFKYINF* |
