AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364370 MtrunA17_Chr7g0217561 99.078 217 2 0 1 217 1 217 3.95e-159 438
Msa1364370 MtrunA17_Chr6g0453281 63.713 237 66 4 1 217 1 237 1.21e-99 288
Msa1364370 MtrunA17_Chr2g0287451 42.574 101 46 2 120 217 103 194 9.01e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364370 AT3G01170.1 51.339 224 93 5 1 217 1 215 7.15e-67 205
Msa1364370 AT5G15260.2 51.261 238 91 8 1 217 1 234 9.30e-67 205
Msa1364370 AT5G15260.1 51.261 238 91 8 1 217 1 234 9.30e-67 205
Msa1364370 AT5G19025.1 54.082 98 43 1 120 217 150 245 9.54e-26 100
Msa1364370 AT5G19025.2 53.608 97 43 1 120 216 150 244 4.25e-25 99.4
Msa1364370 AT3G06180.1 53.061 98 44 1 120 217 132 227 7.28e-25 98.2
Msa1364370 AT3G01170.2 39.216 102 56 2 1 97 1 101 2.16e-11 60.1

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGCTGTTGTTATTGCTTT+AGG 0.138895 tig0018118:+30458 Msa1364370:CDS
CAAACTTTGTGGTTTGTTTA+TGG 0.153027 tig0018118:+30526 Msa1364370:CDS
TTGATCTTTCTATTCTTCTT+TGG 0.201359 tig0018118:-30920 None:intergenic
TGTTATTGCTTTAGGGTTCT+TGG 0.201503 tig0018118:+30466 Msa1364370:CDS
TAGCTGTTGTTATTGCTTTA+GGG 0.217546 tig0018118:+30459 Msa1364370:CDS
TTCTTCAATCCCTTACAATT+AGG 0.224249 tig0018118:-30671 None:intergenic
GAATAGAAAGATCAAGAAAT+AGG 0.263550 tig0018118:+30928 Msa1364370:CDS
CTCATCACAAATTGAATATA+TGG 0.264029 tig0018118:-30494 None:intergenic
TCTTCAATCCCTTACAATTA+GGG 0.277107 tig0018118:-30670 None:intergenic
TGTTGGTAGGTTAGAAGTTC+CGG 0.283392 tig0018118:+30895 Msa1364370:CDS
TGTGAAGAATTCGCCGTCTT+TGG 0.288388 tig0018118:+30733 Msa1364370:CDS
AAGAAGATGGCTCCTGTTAA+TGG 0.295596 tig0018118:+30833 Msa1364370:CDS
AGATGGTGGTGGAATTAAAA+AGG 0.303114 tig0018118:+30760 Msa1364370:CDS
TTGTGGTTTGTTTATGGATT+TGG 0.314324 tig0018118:+30532 Msa1364370:CDS
CATGTGCTGTTGTTGCTACA+TGG 0.391115 tig0018118:+30612 Msa1364370:CDS
GTTGGTAGGTTAGAAGTTCC+GGG 0.396075 tig0018118:+30896 Msa1364370:CDS
AGTGAAAGTTTCAAACTTTG+TGG 0.397466 tig0018118:+30515 Msa1364370:CDS
CCTGTGATTTATGTATCTCT+TGG 0.405703 tig0018118:+30578 Msa1364370:CDS
AGAACAGCTCTTCCATTAAC+AGG 0.409782 tig0018118:-30845 None:intergenic
GTGAAGAATTCGCCGTCTTT+GGG 0.417873 tig0018118:+30734 Msa1364370:CDS
ATGTGGGAACCCTAATTGTA+AGG 0.424650 tig0018118:+30661 Msa1364370:CDS
AGAGCTGTTCTAGTTCTTAG+AGG 0.448409 tig0018118:+30857 Msa1364370:CDS
CAACAGATGAGATCAAACTA+TGG 0.452999 tig0018118:-30307 None:intergenic
AGTGTGGTTGTTCTGTTGGT+AGG 0.472839 tig0018118:+30882 Msa1364370:CDS
GGAAAGTGTGGTTGTTCTGT+TGG 0.477218 tig0018118:+30878 Msa1364370:CDS
GAAGAGAATTGAGTGTCTTA+CGG 0.499754 tig0018118:-30382 None:intergenic
TCGATCCGCGGTTGTTGATG+TGG 0.500037 tig0018118:+30427 Msa1364370:CDS
CTTATACATCAAGGAAATGT+GGG 0.514031 tig0018118:+30645 Msa1364370:CDS
TTTCTATTCTTCTTTGGACC+CGG 0.518411 tig0018118:-30914 None:intergenic
CGAAGCCGAGCTTAAGAAGA+TGG 0.524432 tig0018118:+30820 Msa1364370:CDS
AGGAGCCATCTTCTTAAGCT+CGG 0.536872 tig0018118:-30825 None:intergenic
GCTTATACATCAAGGAAATG+TGG 0.544775 tig0018118:+30644 Msa1364370:CDS
TGTGGGAACCCTAATTGTAA+GGG 0.554639 tig0018118:+30662 Msa1364370:CDS
TCGCCATGGATGATGATGAT+TGG 0.560084 tig0018118:-30331 None:intergenic
TGCTGAGTTTGATATTCAGT+TGG 0.575183 tig0018118:+30697 Msa1364370:CDS
CTAGTTCTTAGAGGAAAGTG+TGG 0.579987 tig0018118:+30866 Msa1364370:CDS
TCCTCGATTTAGCTAAATCG+AGG 0.583685 tig0018118:-30358 None:intergenic
CCGTCTTTGGGTAAAGATGG+TGG 0.586722 tig0018118:+30746 Msa1364370:CDS
CCAAGAGATACATAAATCAC+AGG 0.588766 tig0018118:-30578 None:intergenic
CCCTCGATTTAGCTAAATCG+AGG 0.592975 tig0018118:+30357 Msa1364370:CDS
TGACCAATCATCATCATCCA+TGG 0.601187 tig0018118:+30328 Msa1364370:CDS
CCTCGATTTAGCTAAATCGA+GGG 0.621163 tig0018118:-30357 None:intergenic
CCACCATCTTTACCCAAAGA+CGG 0.627811 tig0018118:-30746 None:intergenic
GATCGAGATCGCTCGCACTG+CGG 0.651752 tig0018118:-30410 None:intergenic
AGTTCGCGATGATGATCACA+AGG 0.663527 tig0018118:-30797 None:intergenic
TCTTTGGGTAAAGATGGTGG+TGG 0.667680 tig0018118:+30749 Msa1364370:CDS
TAAATCGAGGGAATTCGCCA+TGG 0.679775 tig0018118:-30345 None:intergenic
TATAACCACATCAACAACCG+CGG 0.698235 tig0018118:-30432 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATAGCTTATACATCA+AGG + tig0018118:30636-30655 Msa1364370:CDS 20.0%
! CTCATCACAAATTGAATATA+TGG - tig0018118:30497-30516 None:intergenic 25.0%
! TTGATCTTTCTATTCTTCTT+TGG - tig0018118:30923-30942 None:intergenic 25.0%
! ATAGCTGTTGTTATTGCTTT+AGG + tig0018118:30458-30477 Msa1364370:CDS 30.0%
! TAGCTGTTGTTATTGCTTTA+GGG + tig0018118:30459-30478 Msa1364370:CDS 30.0%
AGTGAAAGTTTCAAACTTTG+TGG + tig0018118:30515-30534 Msa1364370:CDS 30.0%
!! CAAACTTTGTGGTTTGTTTA+TGG + tig0018118:30526-30545 Msa1364370:CDS 30.0%
!! TTGTGGTTTGTTTATGGATT+TGG + tig0018118:30532-30551 Msa1364370:CDS 30.0%
CTTATACATCAAGGAAATGT+GGG + tig0018118:30645-30664 Msa1364370:CDS 30.0%
TCTTCAATCCCTTACAATTA+GGG - tig0018118:30673-30692 None:intergenic 30.0%
TTCTTCAATCCCTTACAATT+AGG - tig0018118:30674-30693 None:intergenic 30.0%
CAACAGATGAGATCAAACTA+TGG - tig0018118:30310-30329 None:intergenic 35.0%
GAAGAGAATTGAGTGTCTTA+CGG - tig0018118:30385-30404 None:intergenic 35.0%
!! TGTTATTGCTTTAGGGTTCT+TGG + tig0018118:30466-30485 Msa1364370:CDS 35.0%
CCAAGAGATACATAAATCAC+AGG - tig0018118:30581-30600 None:intergenic 35.0%
CCTGTGATTTATGTATCTCT+TGG + tig0018118:30578-30597 Msa1364370:CDS 35.0%
GCTTATACATCAAGGAAATG+TGG + tig0018118:30644-30663 Msa1364370:CDS 35.0%
TGCTGAGTTTGATATTCAGT+TGG + tig0018118:30697-30716 Msa1364370:CDS 35.0%
!! AGATGGTGGTGGAATTAAAA+AGG + tig0018118:30760-30779 Msa1364370:CDS 35.0%
!! GATGGTGGTGGAATTAAAAA+GGG + tig0018118:30761-30780 Msa1364370:CDS 35.0%
TTTCTATTCTTCTTTGGACC+CGG - tig0018118:30917-30936 None:intergenic 35.0%
TGACCAATCATCATCATCCA+TGG + tig0018118:30328-30347 Msa1364370:CDS 40.0%
CCTCGATTTAGCTAAATCGA+GGG - tig0018118:30358-30377 None:intergenic 40.0%
TCCTCGATTTAGCTAAATCG+AGG - tig0018118:30361-30380 None:intergenic 40.0%
TATAACCACATCAACAACCG+CGG - tig0018118:30435-30454 None:intergenic 40.0%
ATGTGGGAACCCTAATTGTA+AGG + tig0018118:30661-30680 Msa1364370:CDS 40.0%
TGTGGGAACCCTAATTGTAA+GGG + tig0018118:30662-30681 Msa1364370:CDS 40.0%
AAGAAGATGGCTCCTGTTAA+TGG + tig0018118:30833-30852 Msa1364370:CDS 40.0%
AGAACAGCTCTTCCATTAAC+AGG - tig0018118:30848-30867 None:intergenic 40.0%
!! AGAGCTGTTCTAGTTCTTAG+AGG + tig0018118:30857-30876 Msa1364370:CDS 40.0%
! CTAGTTCTTAGAGGAAAGTG+TGG + tig0018118:30866-30885 Msa1364370:CDS 40.0%
! TGTTGGTAGGTTAGAAGTTC+CGG + tig0018118:30895-30914 Msa1364370:CDS 40.0%
! TCGCCATGGATGATGATGAT+TGG - tig0018118:30334-30353 None:intergenic 45.0%
TAAATCGAGGGAATTCGCCA+TGG - tig0018118:30348-30367 None:intergenic 45.0%
CCCTCGATTTAGCTAAATCG+AGG + tig0018118:30357-30376 Msa1364370:CDS 45.0%
! CATGTGCTGTTGTTGCTACA+TGG + tig0018118:30612-30631 Msa1364370:CDS 45.0%
TGTGAAGAATTCGCCGTCTT+TGG + tig0018118:30733-30752 Msa1364370:CDS 45.0%
GTGAAGAATTCGCCGTCTTT+GGG + tig0018118:30734-30753 Msa1364370:CDS 45.0%
CCACCATCTTTACCCAAAGA+CGG - tig0018118:30749-30768 None:intergenic 45.0%
! TCTTTGGGTAAAGATGGTGG+TGG + tig0018118:30749-30768 Msa1364370:CDS 45.0%
AGTTCGCGATGATGATCACA+AGG - tig0018118:30800-30819 None:intergenic 45.0%
AGGAGCCATCTTCTTAAGCT+CGG - tig0018118:30828-30847 None:intergenic 45.0%
! GGAAAGTGTGGTTGTTCTGT+TGG + tig0018118:30878-30897 Msa1364370:CDS 45.0%
AGTGTGGTTGTTCTGTTGGT+AGG + tig0018118:30882-30901 Msa1364370:CDS 45.0%
! GTTGGTAGGTTAGAAGTTCC+GGG + tig0018118:30896-30915 Msa1364370:CDS 45.0%
TCGCCGTCTTTGGGTAAAGA+TGG + tig0018118:30743-30762 Msa1364370:CDS 50.0%
CCGTCTTTGGGTAAAGATGG+TGG + tig0018118:30746-30765 Msa1364370:CDS 50.0%
CGAAGCCGAGCTTAAGAAGA+TGG + tig0018118:30820-30839 Msa1364370:CDS 50.0%
TCGATCCGCGGTTGTTGATG+TGG + tig0018118:30427-30446 Msa1364370:CDS 55.0%
GATCGAGATCGCTCGCACTG+CGG - tig0018118:30413-30432 None:intergenic 60.0%
GTGCGAGCGATCTCGATCCG+CGG + tig0018118:30415-30434 Msa1364370:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0018118 gene 30296 30949 30296 ID=Msa1364370;Name=Msa1364370
tig0018118 mRNA 30296 30949 30296 ID=Msa1364370-mRNA-1;Parent=Msa1364370;Name=Msa1364370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|217
tig0018118 exon 30296 30949 30296 ID=Msa1364370-mRNA-1:exon:13966;Parent=Msa1364370-mRNA-1
tig0018118 CDS 30296 30949 30296 ID=Msa1364370-mRNA-1:cds;Parent=Msa1364370-mRNA-1
Gene Sequence

>Msa1364370

ATGGTAAATTTCCATAGTTTGATCTCATCTGTTGACCAATCATCATCATCCATGGCGAATTCCCTCGATTTAGCTAAATCGAGGAACCGTAAGACACTCAATTCTCTTCAGATTCCGCAGTGCGAGCGATCTCGATCCGCGGTTGTTGATGTGGTTATATTTATAGCTGTTGTTATTGCTTTAGGGTTCTTGGTTTTTCCATATATTCAATTTGTGATGAGTGAAAGTTTCAAACTTTGTGGTTTGTTTATGGATTTGGTGAAAGAAGAAGTTGCTGTTGCTCCTGTGATTTATGTATCTCTTGGAGTTAGTGTTTCATGTGCTGTTGTTGCTACATGGTTTTTTATAGCTTATACATCAAGGAAATGTGGGAACCCTAATTGTAAGGGATTGAAGAATGCTGCTGAGTTTGATATTCAGTTGGAAACTGAAGATTGTGTGAAGAATTCGCCGTCTTTGGGTAAAGATGGTGGTGGAATTAAAAAGGGTCTTTTTAAGATTCCTTGTGATCATCATCGCGAACTCGAAGCCGAGCTTAAGAAGATGGCTCCTGTTAATGGAAGAGCTGTTCTAGTTCTTAGAGGAAAGTGTGGTTGTTCTGTTGGTAGGTTAGAAGTTCCGGGTCCAAAGAAGAATAGAAAGATCAAGAAATAG

Protein sequence

>Msa1364370

MVNFHSLISSVDQSSSSMANSLDLAKSRNRKTLNSLQIPQCERSRSAVVDVVIFIAVVIALGFLVFPYIQFVMSESFKLCGLFMDLVKEEVAVAPVIYVSLGVSVSCAVVATWFFIAYTSRKCGNPNCKGLKNAAEFDIQLETEDCVKNSPSLGKDGGGIKKGLFKIPCDHHRELEAELKKMAPVNGRAVLVLRGKCGCSVGRLEVPGPKKNRKIKK*